scholarly journals Evaluation of the Modulatory Effect of Annona muricata Extracts on the Activity of Some Selected Antibiotics against Biofilm-Forming MRSA

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
David Neglo ◽  
Clement Okraku Tettey ◽  
Edward Ken Essuman ◽  
Justice Dziedzorm Amenu ◽  
Felix Charles Mills-Robertson ◽  
...  
Keyword(s):  
Methicillin Resistant Staphylococcus Aureus ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Hot Water ◽  
Root Extract ◽  
Annona Muricata ◽  
Plant Parts ◽  
Study Support ◽  
Aqueous Root Extract

The study investigated the influence of Annona muricata extracts on the action of selected antibiotics against biofilm-forming MRSA. The various parts of the plant were processed into powder and extracted with ethanol or hot water and then screened for the presence of phytochemicals. The modulatory effect of the Annona muricata extract was also tested on some antibiotics against Methicillin-resistant Staphylococcus aureus (MRSA). The findings from this study revealed that the various parts of the Annona muricata extract (ethanolic and aqueous) contained different proportions of secondary metabolites. Varied antimicrobial activities were observed when the extract of the A. muricata was exposed to MRSA strain at a concentration of 100 mg/mL. The stem recorded the highest (17.00 and 18.00 mm) inhibitory activity against MRSA for both the aqueous and the ethanolic extract, respectively, and this was not different from the control, tetracycline. Again, the results on the modulatory action indicated that out of the 10 extracts of A. muricata, 4 of them antagonized the activity of ampicillin against the tested MRSA by a factor of 0.5 folds and the rest potentiated the drug within 1–4 folds, respectively. On the other hand, the various test extracts significantly potentiated the efficacy of streptomycin and tetracycline against the MRSA by a range of 1–32 folds with the aqueous root extract recording the highest synergistic effect and ethanol seed extract with the least effect. The findings of this study support the antibacterial activities of the A. muricata plant parts.

scholarly journals In-vitro Antimicrobial Activities and Phytochemical Screening of Selected Plant Extracts against Some Medically and Agriculturally Important Pathogens

2020 ◽  
pp. 167-189
Author(s):  
Yirgashewa Asfere ◽  
Ameha Kebede ◽  
Dejene Zinabu
Keyword(s):  
Stem Bark ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Plant Parts ◽  
Antibacterial And Antifungal Activities ◽  
Ruta Chalepensis ◽  
Crude Extracts ◽  
Growth Inhibitory ◽  
Scientific Justification

In Ethiopia, many plants are used for medicinal drive-by old-style naturopaths without any scientific justification for their therapeutic values. The principal aim of this study were to evaluate the in vitro antibacterial and antifungal activities of the leaf, root and stem bark extracts of Rhamnus prinioides (gesho), Justicia schimperiana (sensel) and Ruta chalepensis (Tena adam) against some common pathogenic species of bacteria and fungi. The results of this study shown that the crude extracts of ethanol, methanol, hexane and water crude extracts had antimicrobial activities on most bacterial and fungi species of some solvent extracts. Ethanol and methanol crude extracts had the highest growth inhibitory effects as compared with those of the aqueous and hexane crude extracts. But, the four solvent crude extracts had fewer antimicrobial activities than commercially available drug(chloramphenicol and clotrimazole). Campylobacter jejuni and Staphylococcus aureus were found to be the most vulnerable microbes to the crude ethanol (99.5%). The growth-inhibitory events of the crude extracts were found to be significantly dissimilar for both concentrations (30 and 60 mg/ml) in all plant parts (p < 0.05). In general, this study did not only indicate that the antibacterial activities of R. prinioides (gesho), J. schimperiana (sensel) and R. chalepensis (Tena adam). It also accesses a scientific justification for its old-style use against some diseases.

scholarly journals PHYTOCHEMICALS ANALYSIS AND ANTIMICROBIAL ACTIVITIES OF ECHINOPS ECHINATUS FROM CHOLISTAN DESERT, PAKISTAN

2021 ◽  
Vol 5 ◽  
pp. 21-27
Author(s):  
Muhammad Rafay
Keyword(s):  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Klebsiella Pneumonia ◽  
Root Extract ◽  
Inhibition Activity ◽  
E Coli ◽  
Maximum Inhibition ◽  
Ic50 Value ◽  
Urease Inhibitory

Plants have been used as a source of food as well as medicines by the man throughout history. Echinops echinatus was evaluated for phytochemicals and antibacterial activities against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumonia and Anti-urease inhibition assay activities were tested during the study. The laminar flow cabin was cleaned with 70% ethanol. α-glucosidase inhibition activity was performed according to the standard method with slightly modification. Carbohydrates, phenols and tannins were found positive in leaves, stems and roots. Saponins and glycosides were found in flowers, leaves, roots and stems. While roots and stems revealed the presence of steroids. Terpenoids were found in flowers and leaves. The leaves showed the maximum inhibition zone 10mm against K. pneumonia while against E. coli, S. aureus and P. aeruginosa was found inactive. K. pneumoniae, E. coli and P. aeruginosa were resistant to flowers extract. The root extract showed maximum zone of inhibition 18 mm against S. aureus while against P. aeruginosa and K. pneumonia inhibition zone was 15mm and 14mm respectively. The extracts of leaves, flowers, roots and stem showed positive results for α-glucosidase. The roots extract showed maximum inhibition with 75.3±1.5 with IC50 value of 207.3±1.3 for α-glucosidase. Urease inhibitory activity of stem extracts showed maximum inhibition activity 93.71±0.86 with IC50 value 15.63±0.42. While leaves and roots showed almost same activity 92.45±0.63 and 92.63±0.76, respectively. Echinops echinatus could be considered for further studies in the treatment of various ailments as a natural remedy.

scholarly journals HPLC Analysis and Antimicrobial Screening of Methanol Extract/Fractions of the Root of Millettia aboensis (Hook.f.) Baker against Streptococcus mutans

2020 ◽  
pp. 1-11
Author(s):  
Adeniran J. Ikuesan ◽  
Eze E. Ajaegbu ◽  
Ukachukwu C. Ezeh ◽  
Adaobi J. Dieke ◽  
Adaora L. Onuora ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Methanol Extract ◽  
Extraction Methods ◽  
Antimicrobial Activities ◽  
Root Extract ◽  
Plant Materials ◽  
Aqueous Fraction ◽  
Plant Parts ◽  
Percentage Yield ◽  
Phytochemical Compounds

Millettia aboensis (Hook.f.) Baker belongs to the Leguminosae family, known locally as nduezi in Igbo, erurumesi in Edo, and Òdúdū in Efik. The plant parts have proven to be active against some diseases. The plant was studied for its antimicrobial activity and the phytochemicals present. The plant materials were macerated and fractionated using different extraction methods. The compounds present in the fractions of the stem were detected with the aid of HPLC-DAD. The efficacy of the crude methanol and fractions from the root part was evaluated against Streptococcus mutans. The results showed that the aqueous fraction extract had the highest percentage yield (51.11%), followed by the ethyl acetate (24.10%), butanol (6.63%), and hexane (5.56%), while the methanol extract had a yield of 2.48%. Nine phytochemical compounds were detected from the fractions of the root extract: bis-oxazolidinone derivative – 1, circumdatin F – 2, enniatin B – 3, septicine – 4, orobol 8-C-glucoside – 5, rocaglamide – 6, genistein 6-C-glucoside – 7, 3-phenyl chromen-4-one – 8, and corynesidone D – 9. The methanol extract/fractions of M. aboensis showed no antimicrobial activities on clinical isolates at different concentrations (6.25-50 mg/ml). Based on the poor activity against Strep. mutans, but with promising phytoconstituents present, the extract/fractions need further investigation as regards its utilization against other pathogens.

scholarly journals Annona muricata: Comparative Assessment of the Antibacterial Activities of the Leaf and Stem Extracts against Multiple Antibiotic Resistant Clinical Isolates

2020 ◽  
pp. 12-21
Author(s):  
O. O. Agunloye ◽  
A. K. Onifade
Keyword(s):  
Antibacterial Effect ◽  
Cold Water ◽  
Water Extract ◽  
Antibacterial Activities ◽  
Hot Water ◽  
Annona Muricata ◽  
Antibiotic Resistant ◽  
Crude Extracts ◽  
Hot Water Extract ◽  
Zones Of Inhibition

This study was conducted to determine the antibacterial effect of crude extracts of Annona muricata, comparing the leaf and stem bark extracts using the same extraction solvents. The bacteria isolates were obtained from the hospital. The isolates obtained are Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Proteus mirabilis and Streptococcus pyogene; they were confirmed using standard techniques. Multiple antibiotic-resistant was confirmed in the isolates after antibiotic susceptibility testing. Extracts were prepared from the leaf and stem of A. muricata using ethanol, chloroform, cold water and hot water. The antibacterial activities of the crude extracts were assayed using the agar well diffusion method. The phytochemical screening revealed the presence of saponins, Tannins and flavonoid especially in the ethanol extracts of both leaf and stem part of the plant. All the extract showed varying degrees of antibacterial activities. Chloroform and Hot water rated best for antibacterial activities in this study. A. muricata stem hot water extract has zones of inhibition that ranges from 16.003 ± 0.009 mm to 1.000± 0.006 mm. A. muricata leaf hot water extract has antibacterial effect with zones ranging from 14.500± 0.009 mm to 1.000± 0.006 mm across the isolates. Cold water extracts recorded the lowest zones of inhibition from this study for the stem and Bark antibacterial activities. The stem of A. muricata has higher inhibitory effect on the test isolates compared to the leaf of      A. muricata.

scholarly journals Examination of Phytochemicals, Antioxidant and Antibacterial Activity of Hemigraphisalternate

2019 ◽  
Vol 9 (1S6) ◽  
pp. 98-102
Keyword(s):  
Antimicrobial Agents ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Maximum Activity ◽  
Dependent Manner ◽  
Bacteria And Fungi ◽  
Dose Dependent

Plants as the source of medicine plays avital role in the health service around world. Hemigraphis alternata (Acanthaceae), exotic plants adapt to India, is versatile tropical lower-creeping perennial herbs that reachheight of 15 - 30 centimeters. The matured leaf of Hemigraphis alternata was collected from Kurunthankode , Kanyakumari district. Phytochemical screening of the H.alternata revealed the presence of some phytoconstituents such as carbohydrate, protein, alkaloid, flavonoid, saponin, terpenoid and tannin. Previous report shows that H.coloratacontains similar compounds like saponins, flavonoids and terpenoids. The antioxidant property of H.alternata extract were compared with standard ascorbic acid. The ethanolic extract of H.alternata leaf exhibit good scavenging activity with dose dependent manner. Antimicrobial activities of H.alternata leaf extract show the ethanol extract has the maximum activity against bacteria and fungi. This study revealed the presence of different Phytochemical, antioxidant and antibacterial activities of plant H.alternataand can be suggested that the bioactive components are promising natural antimicrobial agents.

scholarly journals Invitro Antimicrobial Activities of Mitracarpus scaber Against Some Common Bacteria of Aquatic Origin

2021 ◽  
Vol 32 (3) ◽  
pp. 119
Author(s):  
Abdullateef Abiodun Ajadi ◽  
Benjamin Emikpe ◽  
Ahmed Akeem
Keyword(s):  
Antimicrobial Properties ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Ethanolic Extracts ◽  
Zones Of Inhibition ◽  
Mitracarpus Scaber

Some plants have been reported to be of medicinal values and reserve some antimicrobial properties. One of such plants is Mitracarpus scaber and its effect on bacterial growth is evaluated. The study aimed at evaluating the phytochemical analyses and antimicrobial potentials of Mitracarpus scaber against aquatic bacteria including Aeromonas, Bacillus, Vibrio and Staphylococcus Spp. Leaves of Mitracarpus scaber were collected, washed and air dried and phytochemical analysis and antimicrobial investigation of ethanolic and aqueous extracts of the leaves were carried out against a panel of bacteria isolated from diseased catfish from various farms. The phytoconstituents detected include saponins, tannins, flavonoids, tarpenoids, steroids, anthraquinones and alkaloids in both aqueous and ethanolic extracts. Both aqueous and ethanolic extracts of M. scaber showed varying degree of antibacterial activities but ethanolic extract showed a higher activity against the pathogens tested. The ethanolic extract had zones of inhibition similar to that of standard antibiotics (enrofloxacin) across all tested microbes.  The lowest minimum inhibitory concentration of ethanolic extract of M. scaber was against Bacillus sp with 10mg/ml while the highest was 85mg/ml against Staphylococcus species. The results of the assays showed promising evidences that M. scaber is a potential antibacterial agent against aquatic microbes.  However, further studies are recommended to fractionate its constituents and determine the in vitro and in vivo anti-microbial activities and the exact mechanism of action of the constituents.

scholarly journals Chemovariation and antibacterial activity of extracts and isolated compounds from species of Ixora and Greenea (Ixoroideae, Rubiaceae)

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6893 ◽  
Author(s):  
Raveevatoo Buathong ◽  
Voradol Chamchumroon ◽  
Johann Schinnerl ◽  
Markus Bacher ◽  
Wichai Santimaleeworagun ◽  
...  
Keyword(s):  
Stem Bark ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Root Bark ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Methicillin Resistant ◽  
Plant Parts ◽  
Pure Compounds ◽  
Chemical Profiles

Background A large number of secondary metabolites can be obtained from plants used for traditional medicine in two related genera (Ixora and Greenea) in the subfamily Ixoroideae (Rubiaceae), but there are only a few detailed studies on their bioactivities. Therefore, the main goals of this study were to determine the antibacterial activities of lipophilic extracts from plants of some Ixora and Greenea species native to Thailand, and to isolate some pure compounds from those extracts. Moreover, we compared the occurrence of compounds in different plant parts of samples from different habitats to better understand their variation. Methods A total of 56 lipophilic extracts were obtained from the leaves, stem bark, and root bark of eight Ixora and two Greenea species collected at various locations in Thailand. Isolated compounds were identified using nuclear magnetic resonance. Antimicrobial activities were evaluated against four Gram-positive and nine Gram-negative human pathogenic bacterial strains. Results Extracts from I. javanica, I. nigricans, I. brunonis, and G. montana, along with isolated scopoletin, exhibited antibacterial activities against Gram-positive methicillin-resistant Staphylococcus aureus ATCC 43300, with minimum inhibitory concentration values ranging from 64 to 256 µg/mL. The occurrence of scopoletin, isofraxidin, and geniposidic acid in lipophilic extracts showed some variation among different plant parts and species. Conclusions Lipophilic extracts of Ixora and Greenea species have the potential to be developed as anti-Gram-positive agents, in particular to counter infections of methicillin-resistant S. aureus strains. The chemical profiles showed differences between floristic regions but similarity within the same plant parts.

scholarly journals Evaluation of antihyperlipidemic and antioxidant activity of Rubia Cordifolia Linn.

2019 ◽  
Vol 9 (1) ◽  
pp. 211-215
Author(s):  
Priyanka Kumari ◽  
Satish Kumar Sharma ◽  
Snigdha Tiwari
Keyword(s):  
Aqueous Extract ◽  
High Fat Diet ◽  
Low Dose ◽  
Ethanolic Extract ◽  
High Dose ◽  
Root Extract ◽  
High Fat ◽  
Rubia Cordifolia ◽  
Group I ◽  
Aqueous Root Extract

Hyperlipidemia is major problem among population those, who have sedentary life style. The effects of ethanolic and aqueous root extract of Rubiacordifoliain experimentally induced hyperlipidemic rats were investigated. 42 wistar rats of both sexes were used for the study. The animals were completely randomized into seven groups comprising 6 animals each. The groups were treated as follows: Group I: normal diet (ND); Group II: HFD ( Vanaspati ghee + coconut oil mixture in ratio of 3:2 at 10 ml/kg/day); Group III: HFD + Atorvastatin (10 mg/kg/day); Group IV: HFD+ ethanolic extract of roots of Rubia cordifolia low dose(200 mg/kg/day); Group V: HFD + ethanolic extract of roots of Rubia cordifolia high dose (400 mg/kg/day); Group VI: HFD + aqueous extract of roots of Rubia cordifolia low dose (200 mg/kg/day); Group VII: HFD + aqueous extract of roots of Rubia cordifolia high dose (400 mg/kg/day). Hypercholesterolemia was induced by feeding the animals with high fat diet for 21 days before administration of the extract. After 21thday of feeding, administration of extract lasted for 14 days. Preliminary phytochemical screening revealed that the ethanolic and aqueous root extract of Rubia cordifolia contains glycosides, triterpenoids, saponins, alkaloids and flavonoids. Overall, findings from the present study suggest that the antihyperlipidemic activity observed with Atorvastatin (10mg/kg oral) and the ethanolic extracts of Rubia cordifolia (400mg/kg) showed better activity thanaqueous extracts of Rubia cordifolia (200mg/kg). Keywords: Rubia cordifolia, body weight, high fat diet, serum lipid, anti-hyperlipidemic activity.

scholarly journals Phytochemical analysis, proximate composition and antibacterial activities of Ziziphus Species (Z. jujube and Z. spina christi)

2019 ◽  
Vol 4 (1) ◽  
pp. 42
Author(s):  
Kamalu Abdullahi Alhassan ◽  
Aisha Shuaibu Indabawa ◽  
M. Manjur Shah
Keyword(s):  
Aqueous Extract ◽  
Proximate Composition ◽  
Research Work ◽  
Ethanolic Extract ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Phytochemical Analysis ◽  
Bacterial Extract ◽  
E Coli ◽  
Ziziphus Jujube

Despite tremendous progress in human medicines infectious diseases caused by bacteria, fungi, viruses and parasites are still a major threat to public health. Their impact is particularly large in developing countries due to relative unavailability of medicines and emergence of widespread drugs resistance (Zampini 2009).The aim and objectives of this research work was designed to carried out the Phytochemical analysis, proximate composition and evaluate the antimicrobial activities of the Ziziphus jujube and Ziziphus spina christi leaves against clinical bacterial isolates (Escherichia coli, Staphloccoccus  aureus and Klebsiella  pneumoniae) as they were found out that since ancient times to date, they are used in treating various antimicrobial, ailmentand disorders etc. The results of the research shows that, the effect of antibacterial activities of both aqueous and ethanolic extracts of Ziziphus jujube (e extract conc. aqueous; F=119.37, Bacterial extract conc. aqueous; F=1.00 and extract conc. ethanol; F=15.74, Bacterial extract conc. ethanol; F=0.59) are reciprocal proportional to their counterpart, Ziziphus spina-christi (extract conc. aqueous; F=54.96, Bacterial extract conc. aqueous; F=0.94 and extract conc. ethanol; F=81.11, Bacterial extract conc. ethanol; F=1.37). In sum, the minimum inhibitory concentration of Ziziphus jujube shows that, the aqueous extract has M.I.C at range of 11.7 to 8.7mg/ml on all tested bacteria but the ethanolic extract has M.I.C of 14.8 to 8.2mg/ml range on E.coli, Klepsiella spp and S. aureus. While, the minimum inhibitory concentraton of Ziziphus spina-christi shows that the M.I.C of aqueous extract range of 12.8 to 8.3mg/ml on E. coli, Klepsiella spp and S.aureus. But, Ziziphus spina-christi M.I.C of ethanolic extract is 13.5 to 8.8mg/ml on all the tested bacteria. In sum, Zizuphus spina-christi has lower nutritional content and low MIC ethanolic extract than that of Ziziphus jujube.

Antimicrobial Activity of the Extract and Isolated Compounds from Baccharis dracunculifolia D. C. (Asteraceae)

2008 ◽  
Vol 63 (1-2) ◽  
pp. 40-46 ◽  
Author(s):  
Ademar A. da Silva Filho ◽  
João P. B. de Sousa ◽  
Sandra Soares ◽  
Niege A. J. C. Furtado ◽  
Márcio L. Andrade e Silva ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Ursolic Acid ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Phytochemical Study ◽  
Propolis Extract ◽  
Acid Lactone ◽  
First Time ◽  
Mic Value

Baccharis dracunculifolia D.C. (Asteraceae) is the most important plant source of the Brazilian green propolis. Since propolis is known for its antimicrobial activity, the aim of this work was to evaluate the antimicrobial activities of B. dracunculifolia and some of its isolated compounds. The results showed that the leaves extract of B. dracunculifolia (BdE) presents antifungal and antibacterial activities, especially against Candida krusei and Cryptococcus neoformans, for which the BdE showed IC50 values of 65 μg mL −1 and 40 μg mL−1, respectively. In comparison to the BdE, it was observed that the green propolis extract (GPE) showed better antimicrobial activity, displaying an IC50 value of 9 μg mL−1 against C. krusei. Also, a phytochemical study of the BdE was carried out, affording the isolation of ursolic acid (1), 2α-hydroxy-ursolic acid (2), isosakuranetin (3), aromadendrin-4′-methylether (4), baccharin (5), viscidone (6), hautriwaic acid lactone (7), and the clerodane diterpene 8. This is the first time that the presence of compounds 1, 2, and 8 in B. dracunculifolia has been reported. Among the isolated compounds, 1 and 2 showed antibacterial activity against methicillin- resistant Staphylococcus aureus, displaying IC50 values of 5 μg mL−1 and 3 μg mL−1, respectively. 3 was active against C. neoformans, showing an IC50 value of 15 μg mL−1 and a MIC value of 40 μg mL−1, while compounds 4→8 were inactive against all tested microorganisms. The results showed that the BdE, similar to the GPE, displays antimicrobial activity, which may be related to the effect of several compounds present in the crude extract.

Sign in / Sign up

Export Citation Format

Share Document