Efficiency of four sampling methods used to detect two common nosocomial pathogens on textiles

2012 ◽  
Vol 82 (20) ◽  
pp. 2099-2105 ◽  
Author(s):  
Urška Rabuza ◽  
Sonja Šostar-Turk ◽  
Sabina Fijan
Keyword(s):  
Sampling Method ◽  
Sampling Methods ◽  
Sampling Technique ◽  
Nondestructive Method ◽  
Plate Method ◽  
Contact Plate ◽  
Elution Method ◽  
And Staphylococcus Aureus ◽  
Quick Determination

Detecting microorganisms on textiles is useful for many purposes, for example to determine the bioburden before laundering, assess the reduction in bacterial counts in connection with various laundry processes, or trace transfer routes in infection control investigations. Therefore a validated, reproducible and rational method is needed. For sampling microorganisms on textile surfaces the most commonly used method is the contact plate method using RODAC plates, first described by Hall and Hartnett followed by the swab sampling technique. Both methods can only capture microorganisms on the surface of the textiles while microorganisms that have penetrated into the deeper structure of the material will not be detected. In our research the contact plate method and the swabbing technique were compared with two wash-off methods. For the first wash-off method the destructive elution method was used, where microorganisms were eluted from the fabrics by shaking the fabrics for a certain time in an elution medium. For the fourth sampling method a nondestructive method that included a compact test device called Morapex® was used, which is based on forced desorption by pressing the microorganisms through the fabric without destroying the fabric. In our research, two types of microorganisms were included ( Klebsiella pneumoniae and Staphylococcus aureus) that cause common nosocomial infections. The aim of this study was to compare the efficiency of the four sampling methods for detecting microorganisms on textiles and to determine the lowest concentration, which can still be detected. The percentage of microorganisms that were detected by both elution methods was substantially higher than by sampling of fabrics with the contact plate method or swabbing. It can be concluded that a nondestructive method using a modified Morapex® device can be applied for quick determination of the hygienic condition of textiles.

scholarly journals Pengalaman Auditor dan Kepuasan Kerja Sebagai Pemoderasi Pengaruh TBP dan Profesionalisme Pada Kualitas Audit

2019 ◽  
pp. 651
Author(s):  
I Gusti Diah Agung Prabawati Suteja ◽  
Ni Luh Sari Widhiyani
Keyword(s):  
Job Satisfaction ◽  
Regression Analysis ◽  
Data Analysis ◽  
Sampling Method ◽  
Audit Quality ◽  
Time Budget ◽  
Sampling Technique ◽  
Analysis Technique ◽  
Time Budget Pressure

This study aims to determine the effect of time budget pressure (TBP) and professionalism on audit quality with auditor experience and job satisfaction as moderating in KAP in Bali. The population in this study were all auditors who worked in KAP in Bali, amounting to 77 auditors. Determination of samples in this study using non probability sampling method with purposive sampling technique. The sample amounted to 42 auditors. The results of this research questionnaire were measured using a Likert scale. The data analysis technique used is Moderated Regression Analysis. The results of this study indicate that time budget pressure has a negative and significant effect on audit quality; professionalism has a positive and significant effect on audit quality; auditor experience weakens the effect of time budget pressure on audit quality, and strengthens the influence of professionalism on audit quality; and job satisfaction weakens the effect of time budget pressure on audit quality, and strengthens the influence of professionalism on audit quality. Keywords: time budget pressure, professionalism, auditor experience, job satisfaction, audit quality

scholarly journals ANALISIS PENDAPATAN USAHA TERNAK BABI DI KECAMATAN TOMBULU KABUPATEN MINAHASA

ZOOTEC ◽  
2020 ◽  
Vol 40 (2) ◽  
pp. 471
Author(s):  
Yudi P Sinulingga ◽  
N M Santa ◽  
L S Kalangi ◽  
M A.V Manese
Keyword(s):  
Sampling Method ◽  
Business Models ◽  
Secondary Data ◽  
Sampling Technique ◽  
Social Costs ◽  
Primary Data ◽  
Business Income ◽  
Feed Costs ◽  
Quota Sampling

ANALYSIS OF PIG LIVESTOCK BUSINESS INCOME IN TOMBULU DISTRICT MINAHASA REGENCY. The study was conducted in the Tombulu District of Minahasa Regency for one month from January 2020 to February 2020.The data used in this study were primary data and secondary data. The puposive sampling method was applied to determine the sample location in Tombulu District with the number of breeders in Tombulu District were 30 breeders. Therefore, the determination of the sample applied Total Quota Sampling technique. Pigs livestock business models run by breeders were breeding, fattening, combination, and swine pigs. The result showed models that the dominant pig livestock business in Tombulu District was a combination of breeding and fattening. The income earned from the pig livestock business is Rp.39,207,785 / period or Rp3,920,778 / month. The amount of production, feed costs, and social costs are factors that were very influential on the business income of pig livestock.

scholarly journals THE EFFECT OF MANAGERIAL OWNERSHIP, PROFITABILITY, SOLVABILITY AND COMPANY SIZES ON PROFIT MANAGEMENT IN PROPERTY AND REAL ESTATE COMPANIES WHICH ARE IN THE INDONESIAN STOCK EXCHANGE PERIOD 2013 – 2017

2020 ◽  
Vol 18 (2) ◽  
pp. 82
Author(s):  
Rosa Dwi Agsari
Keyword(s):  
Real Estate ◽  
Sampling Method ◽  
Stock Exchange ◽  
Panel Data Analysis ◽  
Predictive Ability ◽  
Sampling Technique ◽  
Total Sample ◽  
Managerial Ownership ◽  
Company Size

This study was conducted to examine the effect of Managerial Ownership, Profitability, Solvency and Company Size variables on Profit Management partially on property and real estate companies listed on the Indonesia Stock Exchange in the period 2013-2017. In addition, to examine the feasibility of a regression model of the influence of variable ownership variables Managerial, Profitability, Solvency and Company Size Management simultaneously on property and real estate companies listed on the Indonesia Stock Exchange in the period 2013-2017. This research was conducted on property and real estate companies on the Indonesia Stock Exchange for the period 2013-2017. The determination of the sample in this study uses a non-probability sampling method with a purposive sampling technique in order to obtain a total sample of 8 companies and a total of 40 observations. The data in this study were analyzed using panel data analysis techniques. The results showed that Managerial Ownership, Solvency and Company Size had no effect on Profit Management. Another variable in this study is that profitability has a positive and significant effect on earnings management. Simultaneously Managerial Ownership, Profitability, Solvency and Company Size Towards Profit Management. The predictive ability of the four variables on Profit Management is 22.84% as shown by the amount of Adjusted R-squared of 0.2284 while the remaining 77.16% is influenced by other factors not included in the research model.

scholarly journals Evaluation of Sampling Methods for Detection of Pathogens from Steel Surfaces; Contact Plates, Foam Swabs, and Flocked Swabs

2020 ◽  
Vol 41 (S1) ◽  
pp. s230-s230
Author(s):  
Rolieria West ◽  
Laura Rose ◽  
Judith Noble-Wang
Keyword(s):  
Stainless Steel ◽  
Steel Surface ◽  
Sampling Methods ◽  
Agar Plate ◽  
Stainless Steel Surface ◽  
Plate Method ◽  
Surface Areas ◽  
Contact Plate ◽  
Composite Contact ◽  
Single Contact

Background: Contaminated healthcare surfaces can serve as reservoirs for the transmission of pathogens. Sensitive sampling methods are needed to investigate sources of pathogens for implementing effective disinfection strategies and thereby preventing environmental transmission. Conventional approaches employ swabs to sample environmental surfaces. Contact plates represent an alternative approach for sampling healthcare surfaces that does not require lab processing, though little is known about their performance. A contact plate is an agar plate that is overfilled with selective or nonselective media. It can be gently applied to the surface, then simply incubated at a temperature optimal for target organism (s), thus saving time and resources. Methods: In this study, contact plates containing trypticase soy agar with 5% sheep blood (TSAII), foam swabs, and flocked swabs were evaluated for their ability to recover 4 pathogens that persist on healthcare surfaces. Stainless-steel coupons (4 in2) were inoculated with the following pathogens (102 CFU): Acinetobacter baumannii (AB, strain type 12), carbapenemase-producing KPC+ Klebsiella pneumoniae (KP; ATCC BAA-1705); methicillin-resistant Staphylococcus aureus (MRSA; ATCC 43300); and vancomycin-resistant Enterococcus faecalis (VRE; Van A + 256). The plates were allowed to dry 1 hour. Sampling with CPs was performed in 2 ways; (1) a single contact plate was used to sample 1 stainless-steel surface and (2) a composite was collected by 3 sequential contact-plate samplings of the same stainless-steel surface. The contact plates were then incubated at 351C. Foam and flocked swabs were premoistened with phosphate-buffered saline + 0.02% polysorbate 80 (PBST) and were used to sample the stainless-steel coupons. Swabs were held for 1 hour and processed by sonication and vortexing in 5 mL of PBST, then the eluent was cultured and CFU counted. Mean percentage recoveries (%R) relative to the inoculum were calculated and compared. Results: When the %R for all 4 pathogens were pooled, the composite contact-plate sampling method yielded the highest, (P < .05) (66.0%; SD, 0.22), followed by the single contact plate method (39.7%; SD, 0.12), foam swab (32.9%; SD, 0.18), and flocked swab (20.3%; SD, 0.20). The composite contact plate method yielded the highest %R for VRE (102.1 %; SD, 0.17), and the lowest %R was observed when using flocked swabs to recover KP (6.3%; SD, 0.05). Conclusions: The contact-plate composite method may provide investigators with minimal environmental microbiology capacity an alternative method for environmental sampling and detection of organisms from surface areas (4 in 2) with low bioburden.Funding: NoneDisclosures: None

scholarly journals Pengaruh Nilai Pelanggan Dan Electronic Word Of Mouth Terhadap Kepuasan Pengunjung Dengan Keputusan Berkunjung Sebagai Variabel Intervening Pada New Marjoly Beach And Resort

2020 ◽  
Vol 4 (1) ◽  
pp. 64-76
Author(s):  
Atika Rosifa ◽  
Lia Suprihartini ◽  
Roni Kurniawan
Keyword(s):  
Data Quality ◽  
Path Analysis ◽  
Customer Value ◽  
Sampling Method ◽  
Word Of Mouth ◽  
Sampling Technique ◽  
Electronic Word Of Mouth ◽  
Analysis Method ◽  
Visitor Satisfaction

This study aims to determine the effect of customer value on visitor satisfaction through visiting decisions, the influence of electronic word of mouth on visitor satisfaction through visiting decisions, the effect of customer value and electronic word of mouth partially on visiting decisions, the influence of customer value and electronic word of mouth partial to visitor satisfaction and the effect of visiting decisions on visitor satisfaction. Determination of the sample using the incidental sampling method, with the sampling technique using the Slovin formula to obtain 96 respondents. The analysis method used in this research is descriptive test, data quality test, classic assumption test, path analysis, and hypothesis testing. The results of the analysis in this study indicate that customer value partially has a significant effect on visitor satisfaction. Partially, electronic word of mouth has a significant effect on visitor satisfaction. Customer value partially has a significant effect on visiting decisions. Partially, electronic word of mouth has no significant effect on visiting decisions. The decision to visit is not able to mediate between customer value and visitor satisfaction and the decision to visit is also unable to mediate between electronic word of mouth and visitor satisfaction. Based on the research results, it is hoped that the new marjoly beach and resort can maintain and increase visitor satisfaction by paying attention to what factors can support customer value and electronic word of mouth.

scholarly journals DETERMINATION OF IMPAIRMENT LOSSES RESERVE AND ITS IMPACT ON INTEREST EARNED AT PT BANK MANDIRI (PERSERO) TBK FROM 2014-2018

2020 ◽  
Vol 6 (11) ◽  
pp. 59-70
Author(s):  
M. Noor Salim ◽  
Andreas Latuperissa
Keyword(s):  
Cross Sections ◽  
Sampling Method ◽  
Time Series Data ◽  
Negative Relationship ◽  
Sampling Technique ◽  
Simple Random Sampling ◽  
Series Data ◽  
Credit Quality ◽  
Debt Payment

The purpose of this study is: 1) To analyze how the different ways of determining impairment losses reserve will affect the interest earned 2) To make recommendation on the best way to determine the impairment losses reserve. This research method uses a quantitative approach based on time series data and cross sections. The population in this study is PT. Bank Mandiri (Persero) Tbk. In this study the sampling technique used probability sampling method. Determination of the sample using the simple random sampling method. Data analysis method in this study uses multiple regression analysis with the help of SPSS version 24.00. The results of the study show some conclusions as follows: 1) Delay in debt payment obligations (PKPU) is proven to have an influence on allowance for impairment losses (CKPN) with a positive relationship. 2) Credit quality (KK) is proven to have an influence on allowance for impairment losses (CKPN) in a negative direction. 3) Delayed debt payment obligations (PKPU) have been proven to influence interest earned with a negative relationship. 4) Credit quality (KK) is proven to have an influence on interest earned and 5) Allowance for impairment losses (CKPN) is proven to have an influence on interest earned with a negative relationship.

scholarly journals PENGARUH PERSEPSI ASAL NEGARA (COUNTRY OF ORIGIN) TERHADAP NIAT BELI YANG DIMEDIASI CITRA MEREK

2019 ◽  
Vol 8 (8) ◽  
pp. 5240
Author(s):  
Ida Bagus Oka Keswara Putra ◽  
Ni Wayan Sri Suprapti
Keyword(s):  
Purchase Intention ◽  
Sampling Method ◽  
Sampling Methods ◽  
Brand Image ◽  
Total Sample ◽  
Purchase Intentions ◽  
National Origin ◽  
Behavioral Observations ◽  
Analysis Technique

This research was conducted in Denpasar City with a total sample of 110 samples. Determination of the sample using non-probability sampling method, namely purposive sampling. Methods of collecting data using non-behavioral observations and questionnaires. The analysis technique used is path analysis technique. Based on the results of the research conducted, it was found that perceptions of national origin had a positive and significant effect on brand image, this means that the better the perception of national origin of Oppo would improve the Oppo brand image. Perceptions of national origin have a positive and significant effect on purchase intentions. This shows consumers 'perceptions of perceptions of national origin influence consumers' buying intention towards Oppo smartphone products. Brand image has a positive and significant effect on purchase intention. This shows that the Oppo brand image has a significant influence on consumers' purchase intentions. Brand image is able to mediate the influence of perceptions of national origin on purchase intention, this means that the brand image mediates the influence of perceptions of national origin into the overall purchase intention. Keywords: perception of national origin, brand image, purchase intention    

scholarly journals Pengaruh Faktor Internal dan Eksternal Terhadap Tingkat Kesehatan Bank

2018 ◽  
Vol 23 (3) ◽  
pp. 359
Author(s):  
Liliani Wijaya, Nuryasman MN
Keyword(s):  
Gross Domestic Product ◽  
Hypothesis Testing ◽  
Commercial Banks ◽  
Sampling Method ◽  
Sampling Technique ◽  
Quantitative Approach ◽  
Domestic Product ◽  
Purposive Sampling ◽  
Insignificant Effect

This search has a purpose to provide emperical evidence about factors that affect health of banks. The examine factors internal on this research are CAMELS and the examine factor external on this research is Gross Domestic Product (GDP). The sample consist of 29 banks.Object of this study is the commercial banks operating in Indonesia in 2012-2015. Determination of sampling technique using purposive sampling method in which the sample is taken with certain criteria. Because the data is used are secondary datawith a quantitative approach, it is necessary to determine the accuracy of the model on some assumption of hypothesis testing using Stata tools. The result showed a partial variable NPL and IER are significant effect on the level of health. While variable CAR, NPM, ROA, LDR, and GDP insignificant effect of the health of the bank. During the period 2012-2015 there were more than 50% showed a stable bank or banks that are in the tittle of “Fit”. Although they are some banks that have increased and decreased to earn the tittle of “unhealthy” for 4 periods.

scholarly journals Pengaruh Relational Capital Terhadap Kesuksesan Usaha Online Shop di Indonesia

2020 ◽  
Vol 2 (1) ◽  
pp. 102
Author(s):  
Welyam Wendy Wongso ◽  
Andi Wijaya
Keyword(s):  
Regression Analysis ◽  
Data Analysis ◽  
Multiple Regression Analysis ◽  
Quantitative Method ◽  
Sampling Method ◽  
Sampling Technique ◽  
Relational Capital ◽  
Analysis Method ◽  
Probability Sampling

The purpose of the study is to analyze the influence of relationships with customers and suppliers, reputation, and location to the success of the business of Online Shop in DKI Jakarta. The method used in this study is a quantitative method with a type of causal research. The population in this study are all entrepreneurs who run an online shop business in DKI Jakarta. Determination of the number of samples is done using probability sampling method with probability sampling technique. The sample in this study was 65 respondents. The data analysis method used in this study is multiple regression analysis using the help of SPSS 24 program. The results show that partially variable relationships with customers and suppliers, reputation, and location have an influence on the success of online shop businesses in DKI Jakarta. Tujuan penelitian adalah untuk menganalisis pengaruh relationships with customers and suppliers, reputation, dan location terhadap kesuksesan usaha Online Shop di DKI Jakarta. Metode yang digunakan dalam penelitian ini adalah metode kuantitatif dengan jenis penelitian sebab akibat. Populasi dalam penelitian ini adalah seluruh wirausaha yang menjalankan usaha online shop di DKI Jakarta. Penentuan jumlah sampel dilakukan menggunakan metode probability sampling dengan teknik probability sampling. Adapun yang menjadi sampel dalam penelitian ini sebanyak 65 responden. Metode analisis data yang digunakan dalam penelitian ini adalah analisis regresi berganda dengan menggunakan bantuan program SPSS 24. Hasil penelitian menunjukkan bahwa secara parsial variabel relationships with customers and suppliers, reputation, dan location memiliki pengaruh terhadap kesuksesan usaha online shop di DKI Jakarta.

scholarly journals ANALISIS DETERMINASI KOMPETENSI PENGELOLA DAN INTENSITAS MENGGUNAKAN LABORATORIUM TERHADAP KETERAMPILAN MENGOPERASIKAN MESIN BALANCING DITINJAU DARI BAKAT MEKANIK PADA MAHASISWA TEKNIK MESIN UNDIKSHA

2019 ◽  
Vol 8 (2) ◽  
pp. 71-78
Author(s):  
Ketut Darma Yuliawan ◽  
Nyoman Dantes ◽  
Ni Ketut Widiartini
Keyword(s):  
Experimental Research ◽  
Partial Correlation ◽  
Sampling Methods ◽  
Sampling Technique ◽  
Data Retrieval ◽  
Purposive Sampling ◽  
Ex Post ◽  
Ex Post Facto ◽  
Student Skill

AbstrakTujuan dari penelitian ini adalah untuk mengetahui seberapa besar determinasi dari kompetensi pengelola laboratorium dan intensitas siswa menggunakan laboratorium ditinjau dari bakat mekanik. Populasi dan sampel dalam penelitian ini adalah mahasiswa prodi Pendidikan Teknik Mesin Universitas Pendidikan Ganesha yang berjumlah 36 orang. Teknik pengambilan sampel yang digunakan adalah purposive sampling. Metode pengambilan data dengan menggunakan tes, kuesioner dan rubrik pengamatan.Penelitian ini adalah penelitian non eksperimen (ex-post facto). Analisis yang digunakan dalam penelitian ini adalah anava satu jalur, anakova satu jalur satu dan dua kovariabel, korelasi parsial dan multiple regresi. Hasil penelitian menunjukkan bahwa : (1) terdapat perbedaan keterampilan mengoperasikan mesin balancing antara siswa yang memiliki bakat mekanik tinggi dan rendah ; (2) terdapat perbedaan keterampilan mengoperasikan mesin balancing antara siswa yang memiliki bakat mekanik tinggi dan rendah setelah dikendalikan oleh kompetensi pengelola laboratorium maupun setelah dikendalikan oleh intensitas siswa menggunakan laboratorium; (3) terdapat determinasi dari kompetensi pengelola laboratorium dan intensitas siswa menggunakan laboratorium terhadap keterampilan siswa mengoperasikan mesin balancing. Kata Kunci : bakat mekanik, kompetensi, intensitas,keterampilan. AbstractThe purpose of this study is to determine how much determination of the competence of laboratory managers and the intensity of students using the laboratory in terms of mechanical talent. Population and sample in this research is student of Mechanical Engineering Education University Ganesha Education which amounts to 36 people. The sampling technique used is purposive sampling. Methods of data retrieval using test, questionnaire and rubric observation. This research is a non experimental research (ex-post facto). Analysis used in this research is one path anava, anakova one lane one and two kovariabel, partial correlation and multiple regresi. The results showed that: (1) there were differences in the skill of operating balancing machines between students with high and low mechanical talents; (2) there is a difference in the skill of operating balancing machines between students who have high and low mechanical talents after controlled by the competence of the laboratory managers and after being controlled by the intensity of the students using the laboratory; (3) there is determination of laboratory manager competence and student intensity using laboratory to student skill to operate balancing machine. Key words : mechanical talent, competence, intensity,skills

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