Rheological and functional characterization of a biopolymer obtained from Beijerinckia sp. / Caracterización reológica y funcional de un biopolímero obtenido de Beijerinckia sp

1996 ◽  
Vol 2 (6) ◽  
pp. 405-411
Author(s):  
L.P. Martínez-Padilla ◽  
N. Cabada-Celis
Keyword(s):  
Food Industry ◽  
Culture Media ◽  
Functional Characterization ◽  
Dry Weight ◽  
Rheological Parameters ◽  
Power Law Model ◽  
Condi Tions ◽  
Almost All ◽  
Potential Use

An exopolysaccharide producer, Beijerinckia sp. was isolated and identified from a sample of soil. A sample of 10.3 g (dry weight) of polysaccharide/L was obtained by fermentation on liquid Czapek culture media supplemented with yeast extract. The functionality of the biopolymer was characterized for its potential use by the food industry. It showed shear thinning behaviour (pseudoplastic). The effects of concentration, temperature and pH were evaluated; almost all of the salts had no effect on rheological parameters of the power law model. There was a linear rela tionship between the percent of remaining emulsion after centrifugation and the polysaccharide biopolymer concentration, demonstrating the stabilizing effect of biopolymer on the emulsion. In contrast, the biopolymer was not able to form a gel under various pH and temperature condi tions, or with the addition of ions.

scholarly journals Incidence and characterisation of Bacillus cereus bacteriophages from Thua Nao, a Thai fermented soybean product

2021 ◽  
Vol 12 (1) ◽  
pp. 85-93
Author(s):  
Wallapat Phongtang ◽  
Ekachai Chukeatirote
Keyword(s):  
Bacillus Cereus ◽  
Food Industry ◽  
Pathogenic Bacteria ◽  
Morphological Analysis ◽  
Food Poisoning ◽  
Fermented Soybean ◽  
Foodborne Pathogenic Bacteria ◽  
Ph Range ◽  
Potential Use

Abstract Bacillus cereus is considered to be an important food poisoning agent causing diarrhea and vomiting. In this study, the occurrence of B. cereus bacteriophages in Thai fermented soybean products (Thua Nao) was studied using five B. cereus sensu lato indicator strains (four B. cereus strains and one B. thuringiensis strain). In a total of 26 Thua Nao samples, there were only two bacteriophages namely BaceFT01 and BaceCM02 exhibiting lytic activity against B. cereus. Morphological analysis revealed that these two bacteriophages belonged to the Myoviridae. Both phages were specific to B. cereus and not able to lyse other tested bacteria including B. licheniformis and B. subtilis. The two phages were able to survive in a pH range between 5 and 12. However, both phages were inactive either by treatment of 50°C for 2 h or exposure of UV for 2 h. It should be noted that both phages were chloroform-insensitive, however. This is the first report describing the presence of bacteriophages in Thua Nao products. The characterization of these two phages is expected to be useful in the food industry for an alternative strategy including the potential use of the phages as a biocontrol candidate against foodborne pathogenic bacteria.

scholarly journals Characteristics of a Series of Three Bacteriophages Infecting Salmonella enterica Strains

2020 ◽  
Vol 21 (17) ◽  
pp. 6152 ◽  
Author(s):  
Katarzyna Kosznik-Kwaśnicka ◽  
Karolina Ciemińska ◽  
Michał Grabski ◽  
Łukasz Grabowski ◽  
Marcin Górniak ◽  
...  
Keyword(s):  
Salmonella Enterica ◽  
Phage Therapy ◽  
Functional Characterization ◽  
Bacterial Biofilm ◽  
Host Cells ◽  
Detailed Characterization ◽  
Food Protection ◽  
Adsorption Rates ◽  
Potential Use

Molecular and functional characterization of a series of three bacteriophages, vB_SenM-1, vB_SenM-2, and vB_SenS-3, infecting various Salmonella enterica serovars and strains is presented. All these phages were able to develop lytically while not forming prophages. Moreover, they were able to survive at pH 3. The phages revealed different host ranges within serovars and strains of S. enterica, different adsorption rates on host cells, and different lytic growth kinetics at various temperatures (in the range of 25 to 42 °C). They efficiently reduced the number of cells in the bacterial biofilm and decreased the biofilm mass. Whole genome sequences of these phages have been determined and analyzed, including their phylogenetic relationships. In conclusion, we have demonstrated detailed characterization of a series of three bacteriophages, vB_SenM-1, vB_SenM-2, and vB_SenS-3, which reveal favorable features in light of their potential use in phage therapy of humans and animals, as well as for food protection purposes.

scholarly journals Functional characterization of canine wild type glucocorticoid receptor and an insertional mutation in a dog

2019 ◽  
Vol 15 (1) ◽  
Author(s):  
Kosei Yamanaka ◽  
Masaru Okuda ◽  
Takuya Mizuno
Keyword(s):  
Glucocorticoid Receptor ◽  
Cushing Syndrome ◽  
Functional Characterization ◽  
Wild Type ◽  
Key Factors ◽  
Truncated Form ◽  
Glucocorticoid Sensitivity ◽  
Almost All

Abstract Background Glucocorticoids, among the most widely utilized drugs in veterinary medicine, are employed to treat a wide variety of diseases; however, their use often induces adverse events in dogs. The efficacy of glucocorticoids usually depends on dosage, although differences in sensitivity to glucocorticoids in individual animals have been reported. Glucocorticoids bind to the cytoplasmic glucocorticoid receptor (GR), which is expressed in almost all cells. These receptors are key factors in determining individual sensitivity to glucocorticoids. This study examined individual differences in glucocorticoid sensitivity in dogs, focusing on reactivity of the GR to prednisolone. Results We first molecularly cloned the GR gene from a healthy dog. We discovered a mutant GR in a dog suspected to have iatrogenic Cushing syndrome. The mutant GR had extra nucleotides between exons 6 and 7, resulting in a truncated form of GR that was 98 amino acids shorter than the wild-type dog GR. The truncated GR exhibited very low reactivity to prednisolone, irrespective of concentration. Conclusions We have identified the truncated form of canine GR in a dog with iatrogenic Cushing syndrome. This truncated form showed the very less sensitivity to glucocorticoid in vitro, unfortunately, we could not elucidate its clinical significance. However, our data is a first report about the function of canine GR, and will facilitate the analysis of canine glucocorticoid sensitivity.

scholarly journals Physicochemical and Techno-Functional Characterization of Native Corn Reintroduced in the Andean Zone of Jujuy, Argentina

Proceedings ◽  
2020 ◽  
Vol 53 (1) ◽  
pp. 7
Author(s):  
María Alejandra Giménez ◽  
Cristina Noemí Segundo ◽  
Manuel Oscar Lobo ◽  
Norma Cristina Sammán
Keyword(s):  
Principal Component Analysis ◽  
Functional Properties ◽  
Food Industry ◽  
Functional Characterization ◽  
Principal Component ◽  
Component Analysis ◽  
High Variability ◽  
Pca Analysis

The chemical and techno-functional properties of nine maize races from the Andean zone of Jujuy, Argentina, in the process of reintroduction, were determined. Principal component analysis (PCA) was applied to establish the differences between them. The breeds studied showed high variability in their chemical and techno-functional properties, which would indicate that their applications in the food industry will also be differentiated. The PCA analysis allowed us to group them into four groups, and the Capia Marron and Culli races showed unique properties, mainly in the formation of gels.

scholarly journals Antioxidant capacity and composition of pitanga seeds

2009 ◽  
Vol 39 (8) ◽  
pp. 2504-2510 ◽  
Author(s):  
Milena Bagetti ◽  
Elizete Maria Pesamosca Facco ◽  
Daniele Bobrowski Rodrigues ◽  
Márcia Vizzotto ◽  
Tatiana Emanuelli
Keyword(s):  
Antioxidant Capacity ◽  
Dietary Fiber ◽  
Food Industry ◽  
Phenolic Content ◽  
Natural Antioxidants ◽  
Insoluble Dietary Fiber ◽  
Low Protein ◽  
Potential Use ◽  
Seed Extracts

Food industry generates a significant amount of seed wastes from the juice production, frozen pulps and jams. Considering that the characterization of wastes is the first step to determine their potential use, the aim of the present study was to determine the composition and the antioxidant capacity of seeds from pitanga fruits with different flesh colors (purple, red and orange). Chemical composition results revealed that pitanga seeds are a good source of insoluble dietary fiber, with low protein and fat levels, and no relevant differences were found among pitanga seeds from different flesh colors. Pitanga seed extracts had powerful antioxidant capacity that was partially correlated to their high phenolic content and showed some variation according to the pitanga flesh colors. Accordingly, it's suggested that this low value waste of pitanga processing, could be used as a source of natural antioxidants and dietary fiber, for animal and/or human nutrition.

scholarly journals Genome-wide analysis of gibberellin-dioxygenases gene family and their responses to GA applications in maize

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0250349
Author(s):  
Jiabin Ci ◽  
Xingyang Wang ◽  
Qi Wang ◽  
Fuxing Zhao ◽  
Wei Yang ◽  
...  
Keyword(s):  
Gene Family ◽  
Plant Development ◽  
Functional Characterization ◽  
Tissue Expression ◽  
Leaf Sheath ◽  
Solid Base ◽  
Specific Expression ◽  
Genome Wide ◽  
Almost All

Gibberellin-dioxygenases genes plays important roles in the regulating plant development. However, Gibberellin-dioxygenases genes are rarely reported in maize, especially response to gibberellin (GA). In present study, 27 Gibberellin-dioxygenases genes were identified in the maize and they were classified into seven subfamilies (I-VII) based on phylogenetic analysis. This result was also further confirmed by their gene structure and conserved motif characteristics. And gibberellin-dioxygenases genes only occurred segmental duplication that occurs most frequently in plants. Furthermore, the gibberellin-dioxygenases genes showed different tissue expression pattern in different tissues and most of the gibberellin-dioxygenases genes showed tissue specific expression. Moreover, almost all the gibberellin-dioxygenases genes were significantly elevated in response to GA except for ZmGA2ox2 and ZmGA20ox10 of 15 gibberellin-dioxygenases genes normally expressed in leaves while 10 and 11 gibberellin-dioxygenases genes showed up and down regulated under GA treatment than that under normal condition in leaf sheath. In addition, we found that ZmGA2ox1, ZmGA2ox4, ZmGA20ox7, ZmGA3ox1 and ZmGA3ox3 might be potential genes for regulating balance of GAs which play essential roles in plant development. These findings will increase our understanding of Gibberellin-dioxygenases gene family in response to GA and will provide a solid base for further functional characterization of Gibberellin-dioxygenases genes in maize.

scholarly journals Systemic Identification and Functional Characterization of Common in Fungal Extracellular Membrane Proteins in Lasiodiplodia theobromae

2021 ◽  
Vol 12 ◽  
Author(s):  
Junbo Peng ◽  
Linna Wu ◽  
Wei Zhang ◽  
Qi Zhang ◽  
Qikai Xing ◽  
...  
Keyword(s):  
Nicotiana Benthamiana ◽  
Functional Characterization ◽  
Pathogenic Fungi ◽  
Opportunistic Pathogen ◽  
Plant Pathogenic Fungi ◽  
Rt Pcr ◽  
Lasiodiplodia Theobromae ◽  
Almost All ◽  
Plant Pathogen Interactions

Plant pathogenic fungi deploy secreted proteins into apoplastic space or intracellular lumen to promote successful infections during plant-pathogen interactions. In the present study, fourteen CFEM domain-containing proteins were systemically identified in Lasiodiplodia theobromae and eight of them were functionally characterized. All eight proteins were confirmed to be secreted into extracellular space by a yeast signal peptide trapping system. The transcriptional levels of most CFEM genes, except for LtCFEM2 and LtCFEM6, were significantly elevated during infection. In addition, almost all LtCFEM genes, apart from LtCFEM2, LtCFEM3, and LtCFEM6, were transcriptionally up-regulated at 35°C in contrast to that at 25°C and 30°C. As two elicitors, LtCFEM1 induced local yellowish phenotype and LtCFEM4 triggered cell death in Nicotiana benthamiana leaves. Furthermore, these proteins displayed distinct subcellular localizations when expressed transiently in N. benthamiana. Moreover, two genes, LtCFEM7 and LtCFEM8, were found to be spliced alternatively by RT-PCR and sequencing. Therefore, our data suggest that LtCFEM proteins play important roles in multiple aspects, including pathogenicity and plant immune response, which will enhance our understanding of the sophisticated pathogenic mechanisms of plant opportunistic pathogen L. theobromae.

Identification of members of the Aspergillus nidulans SREA regulon: genes involved in siderophore biosynthesis and utilization

2002 ◽  
Vol 30 (4) ◽  
pp. 781-783 ◽  
Author(s):  
H. Oberegger ◽  
I. Zadra ◽  
M. Schoeser ◽  
B. Abt ◽  
W. Parson ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Functional Characterization ◽  
Primary Control ◽  
Siderophore Biosynthesis ◽  
Iron Supply ◽  
Potential Impact ◽  
Almost All ◽  
Transcriptional Induction ◽  
Insight Into

Iron is an essential trace element for almost all organisms. However, an excess of this metal within cells can be deleterious on account of catalysis of cell-damaging hydroxyl radicals. Therefore, the concentration of iron within cells is tightly regulated and the primary control occurs by regulating its uptake. Under conditions of low iron availability, most fungi mobilize extracellular iron by excretion of low-molecular-mass ferric iron chelators, termed siderophores. Due to the potential impact of iron metabolism on fungal pathogenicity, a better insight into siderophore-mediated iron uptake is needed. In Aspergillus nidulans, siderophore biosynthesis and uptake are negatively regulated by the GATA-type transcription factor SREA. Hence, genes involved in siderophore biosynthesis and uptake are characterized by transcriptional induction under iron limitation in wild-type strain and de-repression in an sreA-deletion strain under conditions of sufficient iron supply. Such genes have been searched for using different strategies, e.g. differential mRNA display and expression analysis of candidate genes from various A. nidulans sequence databases. The identified genes presumably encode enzymes needed for siderophore biosynthesis, and transporters involved in siderophore uptake and/or excretion. The functional characterization of these genes will help to unravel the pathways involved in siderophore biosynthesis and uptake.

scholarly journals The potential use of probiotic and beneficial bacteria in the Brazilian dairy industry

2018 ◽  
Vol 85 (4) ◽  
pp. 487-496 ◽  
Author(s):  
Monique Colombo ◽  
Svetoslav Dimitrov Todorov ◽  
Monique Eller ◽  
Luís Augusto Nero
Keyword(s):  
Dairy Products ◽  
Food Industry ◽  
Dairy Industry ◽  
Beneficial Bacteria ◽  
Research And Innovation ◽  
Isolation And Characterization ◽  
Potential Source ◽  
Increasing Demand ◽  
Potential Use

Scientific studies demonstrate the importance of intestinal microbiota to human health and how probiotic microorganisms can positively affect health when administered regularly and in adequate amounts. Probiotic bacteria can be part of fermented products and their functional importance is associated mainly with their metabolism. They are thought to benefit individuals to maintain their health and also to strengthen resistance against various types of diseases. The acceptance of probiotic cultures and products by consumers increased when these bacteria were marketed as natural cultures that help in digestion and health. Considering this, the food industry has an increasing demand for new candidates as probiotic cultures, and the dairy industry has a particular interest for fermented milks and other dairy products, since these are the most common food vehicles for probiotic cultures. Therefore, the dairy industries are increasingly seeking to improve their products with these beneficial bacteria. However, the legal peculiarities and excess of control agencies in Brazil makes the registration of these products and the collection of data very complex. Prospective analysis suggests that probiotic foods have the potential to effect a considerable expansion of the dairy industry, allowing the dairy sector to grow and for these products to be increasingly sought by consumers globally. For this, not only actions on research and innovation are necessary, but also official clarifications on the claims for considerations of microbiological security and functionality of these products. This review aims to elucidate important probiotic research regarding the isolation and characterization of beneficial cultures in Brazil, and to demonstrate the relevance of the dairy chain as a potential source of novel cultures for the development of new probiotic products to expand the Brazilian dairy industry.

scholarly journals Extracellular Vesicles from Plants: Current Knowledge and Open Questions

2021 ◽  
Vol 22 (10) ◽  
pp. 5366
Author(s):  
Ornella Urzì ◽  
Stefania Raimondo ◽  
Riccardo Alessandro
Keyword(s):  
Extracellular Vesicles ◽  
Current Knowledge ◽  
Functional Characterization ◽  
Biological Properties ◽  
Controversial Issues ◽  
Animal Cells ◽  
Natural Substances ◽  
Open Questions ◽  
Potential Use

The scientific interest in the beneficial properties of natural substances has been recognized for decades, as well as the growing attention in extracellular vesicles (EVs) released by different organisms, in particular from animal cells. However, there is increasing interest in the isolation and biological and functional characterization of these lipoproteic structures in the plant kingdom. Similar to animal vesicles, these plant-derived extracellular vesicles (PDEVs) exhibit a complex content of small RNAs, proteins, lipids, and other metabolites. This sophisticated composition enables PDEVs to be therapeutically attractive. In this review, we report and discuss current knowledge on PDEVs in terms of isolation, characterization of their content, biological properties, and potential use as drug delivery systems. In conclusion, we outline controversial issues on which the scientific community shall focus the attention shortly.

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