Chemical Composition and Antimicrobial Activity of Wild Garlic Allium ursinum of Bulgarian Origin

The chemical composition of fresh flowers from Allium ursinum (ramsons, bear's garlic, wild garlic) growing in Bulgaria has been studied. Thymidine (1), adenosine (2), astragalin (kaempferol-3-O-β-D-glucopyranoside (3), kaempferol-3-O-D-glucopyranosyl-7-O-D-glucopyranoside (4), kaempferol-3-O-D-neohesperoside (5), and kaempferol-3-O-D-neohesperoside-7-O-D-glucopyranoside (6) were isolated from the n-butanol extract and identified by different spectroscopic and spectrometric methods. Thymine (7), uridine (8), uracil (9) and 5-chloro-uridine (10) were detected in the same extract by GC-MS. This is the first report of the occurrence of 1, 2, 4, 7 – 10 in the flowers of A. ursinum. GC-MS of the volatile components of fresh flowers and leaves from the same plant revealed a high content of sulfur compounds, some of which are reported for the first time for A. ursinum. The antimicrobial activities of extracts from fresh flowers and leaves of A. ursinum have been tested; some extracts exhibited moderate antifungal properties.

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The First Report on Chemical Composition and Antimicrobial Activity of Artemisia scoparia Waldst. et Kit. Extracts

Natural Product Communications ◽

10.1177/1934578x20915034 ◽

2020 ◽

Vol 15 (3) ◽

pp. 1934578X2091503 ◽

Cited By ~ 1

Author(s):

Gordana S. Stojanović ◽

Jovana D. Ickovski ◽

Aleksandra S. Đorđević ◽

Goran M. Petrović ◽

Katarina D. Stepić ◽

...

Keyword(s):

Gas Chromatography ◽

Antimicrobial Activity ◽

Chemical Composition ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Volatile Components ◽

Bacterial Strains ◽

First Report ◽

Main Compound ◽

Artemisia Scoparia

Volatiles of diethyl ether extract (DE), ethyl acetate extract (EE), and hexane extract (HE) of Artemisia scoparia Waldst. et Kit. were analyzed by gas chromatography with flame ionization detector and gas chromatography-mass spectrometry. In both DE and EE, the main compound was scoparone (24.0% and 86.1%, respectively) while in the HE, alkanes were dominant with nonacosane as the most represented (19.4%). Antimicrobial activity was tested against 4 bacterial strains and 1 fungal strain using disc-diffusion method. Tested samples were inactive against Gram-negative bacteria and they exhibited activity against Gram-positive bacteria and yeast Candida albicans. This is the first report on the chemical composition of volatile components and antimicrobial activity of DE, EE, and HE of A. scoparia Waldst. et Kit.

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In Vitro Antimicrobial Activity of Essential Oil Extracted from Leaves of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le in Vietnam

Plants ◽

10.3390/plants9040453 ◽

2020 ◽

Vol 9 (4) ◽

pp. 453 ◽

Cited By ~ 8

Author(s):

Nhan Trong Le ◽

Duc Viet Ho ◽

Tuan Quoc Doan ◽

Anh Tuan Le ◽

Ain Raal ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oil ◽

Inhibitory Concentration ◽

Antimicrobial Activities ◽

Antiviral Effects ◽

Scientific Report ◽

Main Components ◽

Hiv 1

The present study aimed to determine the antimicrobial activity and chemical composition of leaves-extracted essential oil of Leoheo domatiophorus Chaowasku, D.T. Ngo and H.T. Le (L. domatiophorus), including antibacterial, antimycotic, antitrichomonas and antiviral effects. The essential oil was obtained using hydrodistillation, with an average yield of 0.34 ± 0.01% (v/w, dry leaves). There were 52 constituents as identified by GC/MS with available authentic standards, representing 96.74% of the entire leaves oil. The essential oil was comprised of three main components, namely viridiflorene (16.47%), (-)-δ-cadinene (15.58%) and γ-muurolene (8.00%). The oil showed good antimicrobial activities against several species: Gram-positive strains: Staphylococcus aureus (two strains) and Enterococcus faecalis, with Minimum Inhibitory Concentration (MIC) and Minimum Lethal Concentration (MLC) values from 0.25 to 1% (v/v); Gram-negative strains such as Escherichia coli (two strains), Pseudomonas aeruginosa (two strains) and Klebsiella pneumoniae, with MIC and MLC values between 2% and 8% (v/v); and finally Candida species, having MIC and MLC between 0.12 and 4% (v/v).Antitrichomonas activity of the oil was also undertaken, showing IC50, IC90 and MLC values of 0.008%, 0.016% and 0.03% (v/v), respectively, after 48h of incubation. The essential oil resultedin being completely ineffective against tested viruses, ssRNA+ (HIV-1, YFV, BVDV, Sb-1, CV-B4), ssRNA- (hRSVA2, VSV), dsRNA (Reo-1), and dsDNA (HSV-1, VV) viruses with EC50 values over 100 µg/mL. This is the first, yet comprehensive, scientific report about the chemical composition and pharmacological properties of the essential oil in L. domatiophorus.

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Comparative Study of Chemical Composition and Biological Activity of Yellow, Green, Brown, and Red Brazilian Propolis

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2016/6057650 ◽

2016 ◽

Vol 2016 ◽

pp. 1-11 ◽

Cited By ~ 27

Author(s):

Christiane Schineider Machado ◽

João Benhur Mokochinski ◽

Tatiana Onofre de Lira ◽

Fátima de Cassia Evangelista de Oliveira ◽

Magda Vieira Cardoso ◽

...

Keyword(s):

Biological Activity ◽

Chemical Composition ◽

Quality Standard ◽

Antimicrobial Activities ◽

Mato Grosso ◽

Gram Positive Bacteria ◽

H Nmr ◽

Chemical Profiles ◽

Brazilian Propolis ◽

First Time

The chemical composition and biological activity of a sample of yellow propolis from Mato Grosso do Sul, Brazil (EEP-Y MS), were investigated for the first time and compared with green, brown, and red types of Brazilian propolis and with a sample of yellow propolis from Cuba. Overall, EEP-Y MS had different qualitative chemical profiles, as well as different cytotoxic and antimicrobial activities when compared to the other types of propolis assessed in this study and it is a different chemotype of Brazilian propolis. Absence of phenolic compounds and the presence of mixtures of aliphatic compounds in yellow propolis were determined by analysing1H-NMR spectra and fifteen terpenes were identified by GC-MS. EEP-Y MS showed cytotoxic activity against human tumour strain OVCAR-8 but was not active against Gram-negative or Gram-positive bacteria. Our results confirm the difficulty of establishing a uniform quality standard for propolis from diverse geographical origins. The most appropriate pharmacological applications of yellow types of propolis must be further investigated.

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Antimicrobial Activity and Chemical Composition of Essential Oil of Eupatorium Glutinosum (Lam.)

Natural Product Communications ◽

10.1177/1934578x0600100811 ◽

2006 ◽

Vol 1 (8) ◽

pp. 1934578X0600100

Author(s):

Hesham R. El-Seedi

Keyword(s):

Chemical Composition ◽

Essential Oil ◽

Steam Distillation ◽

Antimicrobial Activities ◽

Plant Residue ◽

Volatile Fraction ◽

Positive Bacterium ◽

Medicinal Uses ◽

Study Support ◽

First Time

The essential oil obtained by steam distillation from the leaves and twigs of Eupatorium glutinosum Lam. (Asteraceae) showed antimicrobial activities against a Gram-positive bacterium, three Gram–negative bacteria and three fungi. The results from this study support the vernacular medicinal uses of the plant in folkloric medicine. The chemical composition of the essential oil was analysed by GC and GC-MS, resulting in the identification of 24 constituents accounting for 92.6% of the total mass. The major active component, carvacrol, was isolated using MPLC and characterized by NMR and MS analysis. β-Glucosidase-treatment of the aqueous plant residue yielded a volatile fraction that did not show antimicrobial activitiy, in which the major components were octen-3-ol and (Z)-hex-2-en-1-ol. The essential oil from the species is reported for the first time.

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Screening of antimicrobial activity of essential oil and methanol extract of Satureja hortensis on foodborne bacteria and fungi

Czech Journal of Food Sciences ◽

10.17221/753-cjfs ◽

2008 ◽

Vol 25 (No. 2) ◽

pp. 81-89 ◽

Cited By ~ 43

Author(s):

A. Adiguzel ◽

H. Ozer ◽

H. Kilic ◽

B. Cetin

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Minimum Inhibitory Concentration ◽

Essential Oil ◽

Inhibitory Concentration ◽

Methanol Extract ◽

Antimicrobial Activities ◽

Satureja Hortensis ◽

Bacteria And Fungi

The present work reports the in vitro antimicrobial activities of the essential oil and methanol extract from Satureja hortensis as well as the content of its essential oil. The chemical composition of hydrodistilled essential oil of Satureja hortensis was analysed by means of GC-MS. Thirty constituents were identified. The main constituents of the oil were thymol (40.54%), γ-terpinene (18.56%), carvacrol (13.98%), and p-cymene (8.97). The essential oil of Satureja hortensis exhibited the activity against 25 bacteria, 8 fungi, and a yeast, C. albicans; exerting the Minimum Inhibitory Concentration values (MIC) ranging from 15.62 to 250 µl/ml. Similarly, methanol extract of the plant also showed antimicrobial activity.

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Chemical composition, antioxidant and antimicrobial activities of essential oil of Thymus algeriensis wild-growing in Libya

Open Life Sciences ◽

10.2478/s11535-013-0150-0 ◽

2013 ◽

Vol 8 (5) ◽

pp. 504-511 ◽

Cited By ~ 8

Author(s):

Abdulhmid Giweli ◽

Ana Džamić ◽

Marina Soković ◽

Mihailo Ristić ◽

Petar Marin

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Chemical Composition ◽

Essential Oil ◽

Antimicrobial Activities ◽

Practical Application ◽

Dpph Assay ◽

High Antimicrobial Activity ◽

Thymus Algeriensis ◽

Antioxidant And Antimicrobial Activities

AbstractThe composition of essential oil isolated from Thymus algeriensis growing wild in Libya was analyzed by GC and GC-MS. The essential oil was characterized with thymol (38.50%) as the major component. The oil was screened for antioxidant activity using DPPH assay, and compared to thymol and carvacrol. Antioxidant activity was high, with the IC50 of 0.299 mg/ml, compared to 0.403 and 0.105 mg/ml for thymol and carvacrol, and 0.0717 mg/ml for BHA. In addition, antimicrobial activity was tested against eight bacteria and eight fungi. T. algeriensis oil showed inhibitory activity against tested bacteria at 0.001–0.05 mg/ml, while bactericidal activity (MBC) was achieved at 0.0025–0.05 mg/ml. For antifungal activity MICs ranged 0.0005–0.025 mg/ml and MFC 0.001–0.05 mg/ml. High antimicrobial activity against the fungi in particular suggests that the essential oil of Thymus algeriensis could have a useful practical application.

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Effect of the Distillation Time on the Chemical Composition, Antioxidant Potential and Antimicrobial Activity of Essential Oils from Different Cannabis sativa L. Cultivars

Molecules ◽

10.3390/molecules26164770 ◽

2021 ◽

Vol 26 (16) ◽

pp. 4770

Author(s):

Sara Palmieri ◽

Francesca Maggio ◽

Marika Pellegrini ◽

Antonella Ricci ◽

Annalisa Serio ◽

...

Keyword(s):

Antimicrobial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Cannabis Sativa ◽

Central Italy ◽

Antioxidant Potential ◽

Volatile Fraction ◽

Food Borne ◽

Spoilage Bacteria ◽

First Time

Within the unavoidable variability of various origins in the characteristics of essential oils, the aim of this study was to evaluate the effect of the distillation time on the chemical composition and biological activity of Cannabis sativa essential oils (EOs). The dry inflorescences came from Carmagnola, Kompolti, Futura 75, Gran Sasso Kush and Carmagnola Lemon varieties from Abruzzo region (Central Italy), the last two being new cultivar here described for the first time. EOs were collected at 2 h and 4 h of distillation; GC/MS technique was applied to characterize their volatile fraction. The EOs were evaluated for total polyphenol content (TPC), antioxidant capacity (AOC) and antimicrobial activity against food-borne pathogens and spoilage bacteria. The time of distillation particularly influenced EOs chemical composition, extracting more or less terpenic components, but generally enriching with minor sesquiterpenes and cannabidiol. A logical response in ratio of time was observed for antioxidant potential, being the essential oils at 4 h of distillation more active than those distilled for 2 h, and particularly Futura 75. Conversely, except for Futura 75, the effect of time on the antimicrobial activity was variable and requires further investigations; nevertheless, the inhibitory activity of all EOs against Pseudomonas fluorescens P34 was an interesting result.

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An Analysis of Volatile Components of the Liverworts Dumortiera hirsuta subsp. hirsuta and Dumortiera hirsuta subsp. nepalensis (Dumortieraceae) from Panama and Taxonomic Observations on the Species

Natural Product Communications ◽

10.1177/1934578x1801300827 ◽

2018 ◽

Vol 13 (8) ◽

pp. 1934578X1801300 ◽

Cited By ~ 1

Author(s):

Armando A. Durant-Archibold ◽

Noris Salazar Allen ◽

Anette Garrido ◽

Jose Gudiño Ledezma ◽

Mahabir P. Gupta

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Chemical Composition ◽

Solid Phase Microextraction ◽

Solid Phase ◽

Gas Chromatography Mass Spectrometry ◽

Volatile Components ◽

Chemical Markers ◽

Headspace Solid Phase Microextraction ◽

First Time

We report for the first time the chemical composition of volatile components (VOCs) of two subspecies, D. hirsuta subsp. hirsuta and D. hirsuta subsp. nepalensis, of the liverwort Dumortiera hirsuta from Panama by using headspace-solid phase microextraction-gas chromatography-mass spectrometry in order to assess distinguishing markers between the two species. Forty VOCs were identified in total for both subspecies. Of these, 34 are reported for the first time in D. hirsuta. Furthermore, both subspecies showed clear differences in the type and amount of VOCs. The major compounds in D. hirsuta subsp. hirsuta were α-gurjunene, β-selinene, α-guaiene, α-humulene and β-caryophyllene; while in D. hirsuta subsp. nepalensis were ledene, α-gurjunene, β-caryophyllene and α-guaiene, respectively. Two oxygenated sesquiterpenes, globulol and nerolidol, could be considered as possible distinguishing chemical markers between these two subspecies. We conclude that both morphotypes of D. hirsuta are chemically different.

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Chemical Composition and Antimicrobial Activities of the Essential Oils from Ocimum Selloi and Hesperozygis myrtoides

Natural Product Communications ◽

10.1177/1934578x1100600726 ◽

2011 ◽

Vol 6 (7) ◽

pp. 1934578X1100600 ◽

Cited By ~ 2

Author(s):

Márcia G. Martini ◽

Humberto R. Bizzo ◽

Davyson de L. Moreira ◽

Paulo M. Neufeld ◽

Simone N. Miranda ◽

...

Keyword(s):

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Minimum Inhibitory Concentration ◽

Essential Oils ◽

Inhibitory Concentration ◽

Antimicrobial Effect ◽

Antimicrobial Activities ◽

Open Air Markets ◽

Janeiro State

Ocimum selloi, a traditional medicinal plant from Brazil, is sold in open-air markets at Rio de Janeiro State. Hesperozygis myrtoides is a very aromatic small bush found in the State of Minas Gerais, Brazil, growing at an altitude of 1800m. The chemical composition of both essential oils was analyzed as well as their antimicrobial activity against fungi and bacteria. For all specimens of Ocimum selloi obtained at open-air markets, methylchavicol was major compound found (93.6% to 97.6%) in their essential oils. The major compounds identified in the oil of H. myrtoides were pulegone (44.4%), isomenthone (32.7%), and limonene (3.5%). Both oils displayed antimicrobial activity against all tested microorganisms but Candida albicans was the most susceptible one. Combinations of the two oils in different proportions were tested to verify their antimicrobial effect against C. albicans, which, however, was not modified in any of the concentrations tested. The minimum inhibitory concentration (MIC) was determined to confirm the antimicrobial activity against C. albicans as well as other clinical isolates ( C. glabrata, C. krusei, C. parapsilosis and C. tropicalis).

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Nutritional Value and Antimicrobial Activity of Pittosporum angustifolium (Gumby Gumby), an Australian Indigenous Plant

Foods ◽

10.3390/foods9070887 ◽

2020 ◽

Vol 9 (7) ◽

pp. 887

Author(s):

Anh Dao Thi Phan ◽

Mridusmita Chaliha ◽

Hung Trieu Hong ◽

Ujang Tinggi ◽

Michael E. Netzel ◽

...

Keyword(s):

Antimicrobial Activity ◽

Bioactive Compounds ◽

Nutritional Value ◽

Geographic Location ◽

Dry Weight ◽

Antimicrobial Activities ◽

Endemic Plant ◽

Indigenous Plant ◽

Macro And Micronutrients ◽

First Time

The indigenous endemic plant P. angustifolium has received attention for nutraceutical and therapeutic applications in Australia. This study investigates for the first time the nutritional value (macro- and micronutrients, minerals, trace elements, polyphenols, carotenoids, saponins and antioxidant capacity) and antimicrobial activity of different botanical parts of P. angustifolium, either collected from the wild or cultivated. Different botanical tissues, geographic location and growing condition (wild vs. cultivated) showed significant (p < 0.05) effects on the tested bioactive compounds, with the leaves having significantly (p < 0.05) higher levels than the stems. Saponins and polyphenols could be identified as the main bioactive compounds in the leaves with up to 4% per dry weight. The extracts of P. angustifolium leaves and stems showed strong antioxidant and antimicrobial activities, especially against Candida albicans. These activities correlated (R2 = 0.64–0.92; p < 0.05) with the levels of polyphenols and saponins, indicating their biologic potential. Findings from this study may provide information for future applications of P. angustifolium in the functional ingredient or nutraceutical industry.

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