scholarly journals Prospection of Effects of Guava Leaves Infusion: Antigenotoxic Action and Enzymatic Inhibition

2017 ◽  
Vol 12 (6) ◽  
pp. 1934578X1701200
Author(s):  
Pedro H S Cesar ◽  
Marcus V C Trento ◽  
Daniela A Oliveira ◽  
Anderson A Simão ◽  
Larissa F A Vieira ◽  
...  
Keyword(s):  
Protective Action ◽  
Genetic Material ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Animal Cells ◽  
Enzymatic Inhibition ◽  
Bothrops Alternatus ◽  
Guava Leaves ◽  
Guava Leaf

Several forms of Psidium guajava L. (guava) leaf preparations have been widely used for prevention and treatment of illnesses. However, researches about the protective action of guava's natural products on the genetic material of animal cells is scarce. Accordingly, the aim of this study was to evaluate the antigenotoxic potential of infusions of guava leaves from three cultivars (Pedro Sato, Paluma and Roxa) against DNA damage induced by Doxorubicin (DXR) in human leukocytes, and their effects on enzymatic inhibition. To assess the antigenotoxic potential of these infusions, a comet assay (single cell electrophoresis) was conducted. In addition, their inhibitory potential on phospholipase and hemolytic activities was assessed. The evaluated infusion volumes (25, 50 and 300μL) presented inhibitions around 75% of the DXR-induced damage frequencies. Their protective effect was demonstrated by arbitrary unit calculations, with values between 52.51 and 66.60 for all treatments, 250.51 for positive control (DXR), and 45.49 for phosphate buffered saline (negative control). The infusions significantly inhibited phospholipase and hemolytic activities induced by Bothrops alternatus and B. moojeni venoms, with inhibitions ≥ 50% for both activities induced by B. alternatus. The infusions of P. guajava from the cultivars analyzed present antigenotoxic potential, but also demonstrate potential of enzymatic inhibition on A2 phospholipases and proteases.

scholarly journals UJI EFEK EKSTRAK ETANOL DAUN JAMBU BIJI (Psidium guajava L) TERHADAP KADAR KOLESTEROL TOTAL TIKUS WISTAR (Rattus norvegicus)

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Irianto Girik Allo ◽  
Pemsi Mona Wowor ◽  
Henoch Awaloei
Keyword(s):  
Total Cholesterol ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Cholesterol Levels ◽  
Guava Leaves ◽  
Guava Leaf

Abstract: This research aims to determine the effect of ethanol extract of guava leaves on levels of total cholesterol of rat that is induced propylthiouracil. This was an experimental research. The sample used was 15 rats were divided into 5 groups (1 group is consist of 3 rats), namely: 1) positive control, 2) extract dose of 200 mg/kgBB, 3) extract dose of 400 mg/kgBB, 4) extract dose of 800 mg/kgBB and 5) negative control. Group 1 to 4 are given propylthiouracil given for 14 days, then given treatment appropiate with the group. The result show that: 1) the average of total cholesterol levels increased after administration propylthiouracil from 156.34 mg/dL to 247.84 mg/dL, 2) at a dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg/kgBB showed a decline in total cholesterol levels by 32.73%, 58.73% and 47.26%, 3) the positive control decreased total cholesterol levels by 39.46% and 4) total cholesterol levels on negative is unstable (there are up and some are down). The result of this research concluse that: guava leaf extract at dose of 200 mg/kgBB, 400 mg/kgBB and 800 mg kgBB have an effect in lowering total cholesterol of rat had been induced prophylthiouracil. Keywords: guava leaves, total cholesterol.     Abstrak: Penelitian ini bertujuan untuk mengetahui efek dari ekstrak etanol daun jambu biji terhadap kadar kolesterol total tikus wistar yang diinduksi propiltiourasil. Penelitian ini merupakan penelitian eksperimental. Sampel yang digunakan berjumlah 15 tikus wistar yang dibagi menjadi 5 kelompok (1 kelompok terdiri dari 3 tikus wistar), yaitu: 1) kontrol positif, 2) ekstrak dosis 200 mg/kgBB, 3) ekstrak dosis 400 mg/kgBB, 4) ekstrak dosis 800 mg/kgBB dan 5) kontrol negatif. Kelompok 1 sampai 4 diberikan propiltiourasil selama 14 hari, kemudian diberikan perlakuan sesuai kelompoknya. Hasil penelitian yang diperoleh: 1) rata-rata kadar kolesterol total meningkat setelah pemberian propiltiourasil yaitu dari 156,34 mg/dL menjadi 247,84 mg/dL, 2) pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB menunjukkan terjadi penurunan kadar kolesterol total sebesar 32,73%, 58,73% dan 47,26%, 3) pada kontrol positif terjadi penurunan kadar kolesterol total sebesar 39,46% dan 4) kadar kolesterol total pada kelompok negatif tidak stabil (ada yang naik dan ada yang turun). Hasil penelitian ini menyimpulkan bahwa ekstrak daun jambu biji pada dosis 200 mg/kgBB, 400 mg/kgBB dan 800 mg/kgBB mempunyai efek dalam menurunkan kadar kolesterol total tikus wistar yang telah diinduksi propiltiourasil. Kata kunci: daun jambu biji, kadar kolesterol total.

scholarly journals Efektifitas Lumatan Daun Jambu Biji (Psidium guajava linn) Terhadap Penyembuhan Luka Bakar (Vulnus combustion) Pada Kelinci

2019 ◽  
Vol 1 (2) ◽  
pp. 5-7
Author(s):  
Wahyudi Wahyudi Wahyudi ◽  
Dian Ika Perbina
Keyword(s):  
Digestive System ◽  
Comparison Group ◽  
Chemical Compounds ◽  
Negative Control Group ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Guava Leaf ◽  
And Control

Guava (Guava guajava linn) or guava contains high pectin so it can lower cholesterol, contain tannins that serves to improve the digestive system. Chemical compounds contained in the fruit guava echoes its shape is quersetin i.e. the type of flavonoid compounds a flavonol, flavones, and flavol, which helps them were to treat the fragility of capillaries in the human. The purpose of this research is to know the effectiveness of guava leaf lumatan (Guava guajava linn) in burn healing in rabbits. The methods used in this study was an experimental method using a control group, the Group and the comparison group test (perlakuaan). The results showed that of the negative control group provides the longest healing impact i.e. on the 13th, the positive control healing burns on day 8, and control using lumatan guava leaf healing burns on day 9 control group comparison, bioplacenton indicates days of healing Burns most rapidly healed.

scholarly journals UJI AKTIVITAS ANTIINFLAMASI KOMBINASI DEKOKTA AKAR BELUNTAS (Pluchea indica L.) DAN INFUSA DAUN JAMBU BIJI (Psidium guajava L.) TERHADAP TIKUS PUTIH (Rattus norvegicus) YANG DIINDUKSI KARAGENAN

2015 ◽  
Vol 1 (2) ◽  
pp. 121-125
Author(s):  
Ersamukti Rahmatullah Achmad ◽  
Yuliet Yuliet ◽  
Lutfiana Kusumawati
Keyword(s):  
Rattus Norvegicus ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Percent Inhibition ◽  
Anti Inflammatory ◽  
Guava Leaves ◽  
One Way Anova ◽  
Better Than ◽  
Inflammatory Effect

Marsh fleabane roots (Pluchea indica L.) and guava leaves (Psidium guajava L.) are traditionally used as an anti-inflammatory. The research has been conducted with the aim of knowing the anti-inflammatory effect of the combination of decoction of Marsh fleabane  roots (Pluchea indica L.) and infusion of guava leaves (Psidium guajava L.), and also determining  the effective concentration of such combination. The research used artificial edema method in white rat's leg ( Rattus norvegicus) with the observation for 6 hours on the change of leg volume in white rat.  The measurement of white rat's leg volume used a pletismometer. The type of treatment was devided into 5 groups: negative control (Aquadest), positive control (Na Diclofenac), combination 1 (MFR 10% : IGL 8%), combination 2 (MFR 5% : IGL 5%), and combination 3 (MFR 8% : IGL 10%).  The data obtained were processed using One-Way ANOVA method with the result seen on the percent inhibition of inflammation resulting concentration of MFR 5%: IGL 5% amounting to 23.47%, and subsequently combined with a concentration of MFR 10% : IGL 8% and concentrations MFR 8% : IGL 10% respectively by the percent inhibition of inflammation by 20.70% and 13.75%.  The data obtained show the combination with a concentration of 5% : 5% have anti- inflammatory effect are better than the other combinations as well as comparable to the positive  control

scholarly journals Psidium Guajava L. Extract Against Minimum Inhibitory Concentration of Staphylococcus Aureus

2021 ◽  
Vol 2 (3) ◽  
pp. 22-26
Author(s):  
Lounh Theo Phong ◽  
Nha Thoy Lum
Keyword(s):  
Staphylococcus Aureus ◽  
Bacterial Cell ◽  
Leaf Extract ◽  
Psidium Guajava ◽  
Negative Control ◽  
Essential Components ◽  
Bacterial Cell Membrane ◽  
Guava Leaves ◽  
Guava Leaf

The aim of this research was to evaluate the effects on the growth of Staphylococcus Aureus bacteria in vitro of antibacterial activity of guayava leaf extract (Psidium guajava L.) and guavava leaf extract impact on the growth of staphylococcus Aureus bacteria at different laboratory concentrations. In this experiment, S. Aureus bacteria are being treated to guava leaf extract to identify the lowest inhibitory power for well techniques, and the results have been recorded. The Guava leaf extract (Psidium Guajava L) against Staphylococcus aureus was found to have an inhibitory zone diameter of mm in the absence of tetracycline, and DMSO as a negative control. Findings were observed using the disk diffusion technique or paper disc with concentrations of 1 percent, 3 percent, 5 percent, and 7 percent. Saponins, saponins, alkaloids, and flavonoids found in guava leaves are responsible for their antibacterial effects. Saponins have the potential to disturb the integrity of bacterial cell membranes. This results in bacterial cell membrane destruction, releasing essential components from the cell

scholarly journals UJI EFEKTIVITAS SEDIAAN GEL FRAKSI ETIL ASETAT DAUN JAMBU BIJI (Psidium guajava Linn) TERHADAP PENYEMBUHAN LUKA TERBUKA PADA MENCIT (Mus musculus)

2016 ◽  
Vol 16 (2) ◽  
pp. 23-32
Author(s):  
Lydia Septa Desiyana ◽  
Muhammad Ali Husni ◽  
Seila Zhafira
Keyword(s):  
Wound Healing ◽  
Ethyl Acetate ◽  
Mus Musculus ◽  
Healing Process ◽  
Ethyl Acetate Fraction ◽  
Psidium Guajava ◽  
Positive Control ◽  
Negative Control ◽  
Wound Healing Process ◽  
Guava Leaf

Guava leaf has various benefits, one of them is accelerating the healing process of a wound. This study aims to formulate the ethyl acetate fraction into a gel form and test the effectiveness of gel formulation toward open wounds in 16 male mices (Mus musculus) which made excision 1 cm circular full thickness wound on the back of mice parallel with Os. vetebra. The ethyl acetate fraction of guava leaf has been made into concentrations of 5% and 7% with base gel used as negative control and Bioplacenton as positive control. The evaluation towards ethyl acetate fraction gels showed the gels have green-yellow color, guava odor, homogenous, relevant pH to the skin 6,31-6,51, and spread over 2,8-3 cm (stiff gel). The gels were applied onto the wounds twice a day for about 21 days. The observation was conducted every day towards the scab formation day, scab chipped day, and wound healing day. The data were analyzed statistically using one way ANOVA then continued with Tukey test. The average day for scab formation, scab chipped, and wound healing process, successively are in the negative control i.e. 6,50 ; 13,50 ; 20,25, then positive control of  3,75 ; 11,00 ; 17,25, for gel with 5% concentration,  3,75 ; 9,75 ; 16,50, and gel with 7% concentration,  4,50 ; 12,00 ; 18,25. The statistical result showed that the ethyl acetats fraction  could accelerate the scab formation (p0,05) and the open-wound healing (p0,05) in mice than the control negative (p0,05), but not in the scab exfoliation (p0,05). Gel concentration 5% accelerated faster in forming the scab, flaking off the scab, and  healing the wound compared with the gel concentration of 7%. 

scholarly journals Antibacterial activity of GUAVA, Psidium guajava Linnaeus, leaf extracts on diarrhea-causing enteric bacteria isolated from Seabob shrimp, Xiphopenaeus kroyeri (Heller)

2008 ◽  
Vol 50 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Flávia A. Gonçalves ◽  
Manoel Andrade Neto ◽  
José N. S. Bezerra ◽  
Andrew Macrae ◽  
Oscarina Viana de Sousa ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Essential Oil ◽  
Antimicrobial Effect ◽  
Enteric Bacteria ◽  
Psidium Guajava ◽  
Salmonella Spp ◽  
Leaf Extracts ◽  
Xiphopenaeus Kroyeri ◽  
Guava Leaves ◽  
Guava Leaf

Guava leaf tea of Psidium guajava Linnaeus is commonly used as a medicine against gastroenteritis and child diarrhea by those who cannot afford or do not have access to antibiotics. This study screened the antimicrobial effect of essential oils and methanol, hexane, ethyl acetate extracts from guava leaves. The extracts were tested against diarrhea-causing bacteria: Staphylococcus aureus, Salmonella spp. and Escherichia coli. Strains that were screened included isolates from seabob shrimp, Xiphopenaeus kroyeri (Heller) and laboratory-type strains. Of the bacteria tested, Staphylococcus aureus strains were most inhibited by the extracts. The methanol extract showed greatest bacterial inhibition. No statistically significant differences were observed between the tested extract concentrations and their effect. The essential oil extract showed inhibitory activity against S. aureus and Salmonella spp. The strains isolated from the shrimp showed some resistance to commercially available antibiotics. These data support the use of guava leaf-made medicines in diarrhea cases where access to commercial antibiotics is restricted. In conclusion, guava leaf extracts and essential oil are very active against S. aureus, thus making up important potential sources of new antimicrobial compounds.

scholarly journals The Potential of Guava Leaf Extract as A Natural Preservation for Fish

2020 ◽  
Vol 2 (1) ◽  
pp. 62-69
Author(s):  
Riyanto
Keyword(s):  
Leaf Extract ◽  
Methanolic Extract ◽  
Psidium Guajava ◽  
Control Treatment ◽  
Fish Quality ◽  
High Protein Content ◽  
Fish Samples ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Good Medium

Fish is kinds of food that easy to spoil. High protein content in fish makes the food is a good medium for microorganisms. People commonly buy fish for consumption, however, not all fish are consumed at the same time. Fishes were stored in freezer for several days or preserved with additional chemical preservation. The use of organic preservatives have no harm. This paper discussed guava (Psidium guajava L) leave as natural fish preservation. The objectives were to analyze the potency of the leaves as a natural preservation for mackerel fish (Rastrelliger sp.). Fime parameters used to determine the fish quality as follows : gills, eye, texture, odour and mucilage. The treatments were leaf methanolic extract with doses 0, 20, 40, 60 and 80%. Data were taken on from 1 to 5 days after storage. The results showed that (i) Fish quality was decline and start to depraved at 2 day storage, especially at control treatment (0% extract). Treatment at doses 60 and 80%, the fish quality were still relatively good over 3 days storage. Even though the fish samples have been starting spoilage, those fishes were still available to be consumed. At the same time, with the other treatment, the fish samples have been spoiled and no more available to be consumed. However, at 5th day of storage, all samples at all treatments were spoiled. Overall results, it can be concluded that extract of guava leaves can be has as fish preservativedosage

scholarly journals Preliminary Phitochemical Analysis Of Guava Leaves (Psidium guajava L.) Extract As Antidiarrheal In Calves

2019 ◽  
Vol 3 (2) ◽  
pp. 21
Author(s):  
I Wayan Sudira ◽  
I Made Merdana ◽  
Suci Nur Qurani
Keyword(s):  
Leaf Extract ◽  
Ethanol Extract ◽  
Psidium Guajava ◽  
Phytochemical Analysis ◽  
Pharmacological Effects ◽  
The West ◽  
Active Compounds ◽  
Central Java ◽  
Guava Leaves ◽  
Guava Leaf

Guava plants (Psidium guajava L) are fruit plants originating from South America and can thrive in the territory of Indonesia. Now the biggest center for guava cultivation is spread in DKI Jakarta, West Java, Central Java, East Java, Yogyakarta, Bali, West Nusa Tenggara, Sumatra and Kalimantan. In addition to taking the fruit, guava leaves are also used as a traditional medicine for the treatment of diarrhea in human and animals. This study aims to identify active compounds in guava leaves. That is a secondary  metabolites with pharmacological effects as anti diarrhea such as flavonoid, alkaloid, tannins and essential oils. The leaves sample used in this study were taken from farmers in the West Denpasar area, Bali. Extraction of guava leaf leaves using 96% ethanol, and phytochemical analysis was carried out to detect the presence of active compounds. The data obtained is presented and analyzed descriptively qualitatively. The results showed that the ethanol extract of guava leaves contained active compound inclaude steroid/triterpenoid, phenolics, alkaloids, flavonoids, saponins and tannins. It can be concluded that guava leaf extract contains are potential antidiarrheal

scholarly journals A Study on Bioadsorption of Synthetic Dye Using Guava (Psidium guajava) Leaves

2020 ◽  
Vol 5 (9) ◽  
pp. 215-218
Author(s):  
Manasee Vichare ◽  
Prashant Masali
Keyword(s):  
Psidium Guajava ◽  
Water Bodies ◽  
Brilliant Blue ◽  
Tropical Region ◽  
Coomassie Brilliant Blue ◽  
Leaf Powder ◽  
Blue Solution ◽  
Guava Leaves ◽  
Guava Leaf ◽  
Coomassie Brilliant

Guava (Psidium guajava, white Indian) is common fruit bearing plant found throughout the Indian peninsula. It is said to be native to Mexico and Southern America and now can be found in many of the Asian countries. It is grown in tropical and sub-tropical region around the world. Guava leaves have shown many properties like antibacterial, bioadsorbent, etc. The bioadsorbent property can be exploited to remove coloured residues from water bodies. Problems like water pollution have arisen due to rapid industrialization and urbanization. Various harmful dyes like Coomassie brilliant blue, used in both textile industries and laboratories, are expelled into water bodies. To reduce the effects of such dye on ecology the research was carried out using guava leaves as bioadsorbent. That being the case the Coomassie brilliant blue solution at different pH was treated with guava leaf powder and the decrease in absorbance was noted at 625 nm. The Guava leaves were obtained from Mumbai suburban. The leaves were dried and powdered for further testing as bioadsorbent. All the chemicals have been used were of analytical grade. The study showed high absorption rate in acidic medium as the colour changed from dark blue to light blue. The optimum pH for bio-adsorption was found to be 3. As the amount of guava leaf powder increased, the rate of adsorption also increased. Guava leaf powder is effective as a bioadsorption as rate of adsorption increases with increase in guava leaf powder. With further optimization it can become potential source of adsorbent.

scholarly journals Chimeric synthetic reference standards enable cross-validation of positive and negative controls in SARS-CoV-2 molecular tests

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bindu Swapna Madala ◽  
Andre L. M. Reis ◽  
Ira W. Deveson ◽  
William Rawlinson ◽  
Tim R. Mercer
Keyword(s):  
Cross Validation ◽  
Diagnostic Testing ◽  
Genetic Material ◽  
Positive Control ◽  
Negative Control ◽  
Reference Standards ◽  
Rt Pcr ◽  
Standard Design ◽  
Negative Controls

AbstractDNA synthesis in vitro has enabled the rapid production of reference standards. These are used as controls, and allow measurement and improvement of the accuracy and quality of diagnostic tests. Current reference standards typically represent target genetic material, and act only as positive controls to assess test sensitivity. However, negative controls are also required to evaluate test specificity. Using a pair of chimeric A/B RNA standards, this allowed incorporation of positive and negative controls into diagnostic testing for the Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2). The chimeric standards constituted target regions for RT-PCR primer/probe sets that are joined in tandem across two separate synthetic molecules. Accordingly, a target region that is present in standard A provides a positive control, whilst being absent in standard B, thereby providing a negative control. This design enables cross-validation of positive and negative controls between the paired standards in the same reaction, with identical conditions. This enables control and test failures to be distinguished, increasing confidence in the accuracy of results. The chimeric A/B standards were assessed using the US Centres for Disease Control real-time RT-PCR protocol, and showed results congruent with other commercial controls in detecting SARS-CoV-2 in patient samples. This chimeric reference standard design approach offers extensive flexibility, allowing representation of diverse genetic features and distantly related sequences, even from different organisms.

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