scholarly journals 3D skin models in domestic animals

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Laurent Souci ◽  
Caroline Denesvre
Keyword(s):  
Cell Biology ◽  
Animal Experiments ◽  
Mouse Skin ◽  
3D Models ◽  
Domestic Animals ◽  
The Body ◽  
Societal Pressure ◽  
Potential Applications ◽  
Active Barrier ◽  
Human Skin Models

AbstractThe skin is a passive and active barrier which protects the body from the environment. Its health is essential for the accomplishment of this role. Since several decades, the skin has aroused a strong interest in various fields (for e.g. cell biology, medicine, toxicology, cosmetology, and pharmacology). In contrast to other organs, 3D models were mostly and directly elaborated in humans due to its architectural simplicity and easy accessibility. The development of these models benefited from the societal pressure to reduce animal experiments. In this review, we first describe human and mouse skin structure and the major differences with other mammals and birds. Next, we describe the different 3D human skin models and their main applications. Finally, we review the available models for domestic animals and discuss the current and potential applications.

scholarly journals Perilla frutescens Leaf Extract and Fractions: Polyphenol Composition, Antioxidant, Enzymes (α-Glucosidase, Acetylcholinesterase, and Tyrosinase) Inhibitory, Anticancer, and Antidiabetic Activities

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 315
Author(s):  
Zhenxing Wang ◽  
Zongcai Tu ◽  
Xing Xie ◽  
Hao Cui ◽  
Kin Weng Kong ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Animal Experiments ◽  
Ethyl Acetate Fraction ◽  
The Body ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Glycogen Accumulation ◽  
Inhibitory Ability

This study aims to evaluate the bioactive components, in vitro bioactivities, and in vivo hypoglycemic effect of P. frutescens leaf, which is a traditional medicine-food homology plant. P. frutescens methanol crude extract and its fractions (petroleum ether, chloroform, ethyl acetate, n-butanol fractions, and aqueous phase residue) were prepared by ultrasound-enzyme assisted extraction and liquid–liquid extraction. Among the samples, the ethyl acetate fraction possessed the high total phenolic (440.48 μg GAE/mg DE) and flavonoid content (455.22 μg RE/mg DE), the best antioxidant activity (the DPPH radical, ABTS radical, and superoxide anion scavenging activity, and ferric reducing antioxidant power were 1.71, 1.14, 2.40, 1.29, and 2.4 times higher than that of control Vc, respectively), the most powerful α-glucosidase inhibitory ability with the IC50 value of 190.03 μg/mL which was 2.2-folds higher than control acarbose, the strongest proliferative inhibitory ability against MCF-7 and HepG2 cell with the IC50 values of 37.92 and 13.43 μg/mL, which were considerable with control cisplatin, as well as certain inhibition abilities on acetylcholinesterase and tyrosinase. HPLC analysis showed that the luteolin, rosmarinic acid, rutin, and catechin were the dominant components of the ethyl acetate fraction. Animal experiments further demonstrated that the ethyl acetate fraction could significantly decrease the serum glucose level, food, and water intake of streptozotocin-induced diabetic SD rats, increase the body weight, modulate their serum levels of TC, TG, HDL-C, and LDL-C, improve the histopathology and glycogen accumulation in liver and intestinal tissue. Taken together, P. frutescens leaf exhibits excellent hypoglycemic activity in vitro and in vivo, and could be exploited as a source of natural antidiabetic agent.

scholarly journals Digital 3D models of theropods for approaching body-mass distribution and volume

2021 ◽  
Author(s):  
Matías Reolid ◽  
Francisco J. Cardenal ◽  
Jesús Reolid
Keyword(s):  
Body Length ◽  
Heat Dissipation ◽  
Volume Ratio ◽  
3D Models ◽  
The Body ◽  
3D Analysis ◽  
Skull Length ◽  
Knowing That ◽  
Fossil Bones ◽  
Primitive Forms

AbstractThe aim of this work is to obtain diverse morphometric data from digitized 3D models of scientifically accurate palaeoreconstructions of theropods from eight representative families. The analysed polyvinyl chloride (PVC) models belong to the genera Coelophysis, Dilophosaurus, Ceratosaurus, Allosaurus, Baryonyx, Carnotaurus, Giganotosaurus, and Tyrannosaurus. The scanned 3D models were scaled considering different body-size estimations of the literature. The 3D analysis of these genera provides information on the skull length and body length that allows for recognition of major evolutionary trends. The skull length/body length in the studied genera increases according with the size of the body from the smallest Coelophysis with a ratio of 0.093 to ratios of 0.119–0.120 for Tyrannosaurus and Giganotosaurus, the largest study theropods. The study of photogrammetric 3D models also provides morphometric information that cannot be obtained from the study of bones alone, but knowing that all reconstructions begin from the fossil bones, such as the surface/volume ratio (S/V). For the studied theropod genera surface/volume ratio ranges from 35.21 for Coelophysis to 5.55 for Tyrannosaurus. This parameter, closely related to the heat dissipation, help in the characterization of the metabolism of extinct taxa. Accordingly, slender primitive forms of the Early Jurassic (i.e. Coelophysis and Dilophosaurus) had relatively smaller skulls and higher mass-specific metabolic rates than the robust large theropods of the Cretaceous (i.e. Giganotosaurus and Tyrannosaurus). This work presents a technique that, when applied to proper dinosaur models, provides extent and accurate data that may help in diverse study areas within the dinosaur palaeontology and palaeobiology.

scholarly journals Nanocomposite scaffolds for accelerating chronic wound healing by enhancing angiogenesis

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hamed Nosrati ◽  
Reza Aramideh Khouy ◽  
Ali Nosrati ◽  
Mohammad Khodaei ◽  
Mehdi Banitalebi-Dehkordi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Tissue Regeneration ◽  
Molecular Mechanisms ◽  
Healing Process ◽  
The Body ◽  
Key Factors ◽  
Potential Applications ◽  
Nanocomposite Scaffolds

AbstractSkin is the body’s first barrier against external pathogens that maintains the homeostasis of the body. Any serious damage to the skin could have an impact on human health and quality of life. Tissue engineering aims to improve the quality of damaged tissue regeneration. One of the most effective treatments for skin tissue regeneration is to improve angiogenesis during the healing period. Over the last decade, there has been an impressive growth of new potential applications for nanobiomaterials in tissue engineering. Various approaches have been developed to improve the rate and quality of the healing process using angiogenic nanomaterials. In this review, we focused on molecular mechanisms and key factors in angiogenesis, the role of nanobiomaterials in angiogenesis, and scaffold-based tissue engineering approaches for accelerated wound healing based on improved angiogenesis.

scholarly journals Virtual Archaeology of Death and Burial: A Procedure for Integrating 3D Visualization and Analysis in Archaeothanatology

2021 ◽  
Vol 7 (1) ◽  
pp. 540-555
Author(s):  
Hayley L. Mickleburgh ◽  
Liv Nilsson Stutz ◽  
Harry Fokkens
Keyword(s):  
Spatial Information ◽  
3D Visualization ◽  
Rapid Development ◽  
Three Dimensional ◽  
3D Models ◽  
The Body ◽  
Test Case ◽  
Archaeology Of Death ◽  
Human Burials ◽  
3D Information

Abstract The reconstruction of past mortuary rituals and practices increasingly incorporates analysis of the taphonomic history of the grave and buried body, using the framework provided by archaeothanatology. Archaeothanatological analysis relies on interpretation of the three-dimensional (3D) relationship of bones within the grave and traditionally depends on elaborate written descriptions and two-dimensional (2D) images of the remains during excavation to capture this spatial information. With the rapid development of inexpensive 3D tools, digital replicas (3D models) are now commonly available to preserve 3D information on human burials during excavation. A procedure developed using a test case to enhance archaeothanatological analysis and improve post-excavation analysis of human burials is described. Beyond preservation of static spatial information, 3D visualization techniques can be used in archaeothanatology to reconstruct the spatial displacement of bones over time, from deposition of the body to excavation of the skeletonized remains. The purpose of the procedure is to produce 3D simulations to visualize and test archaeothanatological hypotheses, thereby augmenting traditional archaeothanatological analysis. We illustrate our approach with the reconstruction of mortuary practices and burial taphonomy of a Bell Beaker burial from the site of Oostwoud-Tuithoorn, West-Frisia, the Netherlands. This case study was selected as the test case because of its relatively complete context information. The test case shows the potential for application of the procedure to older 2D field documentation, even when the amount and detail of documentation is less than ideal.

Cellular and immunological basis of the host-parasite relationship during infection withNeospora caninum

Parasitology ◽  
2006 ◽  
Vol 133 (3) ◽  
pp. 261-278 ◽  
Author(s):  
A. HEMPHILL ◽  
N. VONLAUFEN ◽  
A. NAGULESWARAN
Keyword(s):  
Host Cell ◽  
Cell Biology ◽  
Neospora Caninum ◽  
Host Cells ◽  
Term Survival ◽  
Prevention Of Infection ◽  
Parasite Relationship ◽  
Potential Applications

Neospora caninumis an apicomplexan parasite that is closely related toToxoplasma gondii, the causative agent of toxoplasmosis in humans and domestic animals. However, in contrast toT. gondii, N. caninumrepresents a major cause of abortion in cattle, pointing towards distinct differences in the biology of these two species. There are 3 distinct key features that represent potential targets for prevention of infection or intervention against disease caused byN. caninum. Firstly, tachyzoites are capable of infecting a large variety of host cellsin vitroandin vivo. Secondly, the parasite exploits its ability to respond to alterations in living conditions by converting into another stage (tachyzoite-to-bradyzoite orvice versa). Thirdly, by analogy withT. gondii, this parasite has evolved mechanisms that modulate its host cells according to its own requirements, and these must, especially in the case of the bradyzoite stage, involve mechanisms that ensure long-term survival of not only the parasite but also of the host cell. In order to elucidate the molecular and cellular bases of these important features ofN. caninum, cell culture-based approaches and laboratory animal models are being exploited. In this review, we will summarize the current achievements related to host cell and parasite cell biology, and will discuss potential applications for prevention of infection and/or disease by reviewing corresponding work performed in murine laboratory infection models and in cattle.

scholarly journals Method for auxiliary use of thermography in diagnosing inflammation in the coxofemoral joint in dogs

2018 ◽  
Vol 39 (4) ◽  
pp. 1565
Author(s):  
Fernanda Lúcia Passos Fukahori ◽  
Daniela Maria Bastos de Souza ◽  
Eduardo Alberto Tudury ◽  
George Chaves Jimenez ◽  
José Ferreira da Silva Neto ◽  
...  
Keyword(s):  
Thermal Emission ◽  
Low Cost ◽  
Joint Inflammation ◽  
Domestic Animals ◽  
The Body ◽  
Joint Diseases ◽  
Infrared Images ◽  
Infrared Thermometry ◽  
Non Invasive ◽  
Sensitivity Specificity

Joint diseases are relatively common in domestic animals, such as dogs. The involved inflammation produces thermal emission, which can be imaged using specific sensors that allow capturing of infrared images. Given that there have been few reports on the use of thermography in the diagnosis of inflammation associated with diseases of the hip joint in dogs, we here propose a method for identification of inflammatory foci in dogs by using infrared thermometry. The present study aimed to find non-invasive and low-cost resources that couldfacilitate a clinical diagnosis in cases withinflammation in the coxofemoral joint of dogs.To this end, we developed a system in whichthe Flir Systems TG165 thermograph is coupled to a black PVC cannula with a 30-cm focus-to-animal distance.External effects of the environment on the temperature of the animalswere compared with the body temperature as measured by a conventional thermometer.Thirty-one dogs with and without inflammation in the coxofemoral joint underwent clinical evaluation.We verified that the temperature registered by the thermograph inthe animals with joint inflammation was significantlydifferentfrom that incontrol animals without inflammation, in the lateral projection.The method showed a sensitivity of 80%, specificity of 87.5%, and accuracy of 83.87%. This standardized method of diagnosis of inflammatory foci in the coxofemoral articulation of dogs by way of thermography showed sensitivity, specificity, and satisfactory accuracy.

scholarly journals On the hygienic rating of silicon compounds in drinking water (literature review)

2021 ◽  
Vol 100 (10) ◽  
pp. 1077-1083
Author(s):  
Yurii A. Rakhmanin ◽  
Natalija A. Egorova ◽  
Rufina I. Mikhailova ◽  
Irina N. Ryzhova ◽  
Marina G. Kochetkova
Keyword(s):  
Drinking Water ◽  
Adult Population ◽  
Animal Experiments ◽  
Age Groups ◽  
Incidence Rates ◽  
The Body ◽  
Unique Element ◽  
Silicon Compounds ◽  
History Of ◽  
Biogeochemical Provinces

Introduction. Silicon is a unique element in its physicochemical properties, almost ubiquitous in water supply sources but is not normalized in drinking water in the world practice of ensuring the harmlessness of water use of the population. In our country, the half-century history of the development of hygienic silicon standards in the water began with the justification of the MPC of silicon-containing reagents used in water treatment. However, over time, doubts have arisen about the suitability of manufactured silicon standards for controlling natural silicon in drinking water. Proponents of the harmlessness of natural soluble forms of silicon advocated the elimination of silicon standards in water. In contrast, researchers of the action of silicon in silicon biogeochemical provinces have constantly revealed its adverse effects on the health of the population living in these regions, confirming this with animal experiments. methods. Literature search methods on Scopus, CyberLeninka, PubMed databases: selective, analytical-synthetic, typological. the main part. The review provides information on soluble forms of silicon, their stability and bioavailability, examines the retrospective and current state of hygienic rationing of silicon in water, discusses the shortcomings of the currently existing MPC of silicon, sodium and potassium silicates in drinking water. A detailed review of studies carried out in the silicon biogeochemical provinces of Chuvashia is given, where the inhabitants of the ecological disaster zone, who consumed drinking water with a profound imbalance of macro-and microelements and a high silicon content relative to the optimum area, observed various metabolic disorders (mineral, lipid, carbohydrate, peroxide), changes in microbiocenosis of the large intestine and immune status, an increase in the incidence rate of the adult population with chronic non-infectious diseases by 2-3 times against the national average, the highest incidence rates in children of all age groups. Conclusions. The review draws attention to the need for expanded studies of the effect on the body of natural silicon in drinking water with the usual balance of trace elements to resolve issues about the standards for natural silicon at the federal and regional levels and to establish differentiated standards for silicon-containing reagents in drinking water.

Biocompatible Cardiac Sensor with Continuous Arrhythmia Monitoring for Human Wellness

2016 ◽  
Vol 835 ◽  
pp. 71-77
Author(s):  
Jong Ha Lee
Keyword(s):  
Atrial Fibrillation ◽  
Power Transmission ◽  
Sampling Rate ◽  
Animal Experiments ◽  
Small Animal ◽  
The Body ◽  
Major Surgery ◽  
Data Sampling ◽  
Integrated Sensor ◽  
Sensor Module

Prolonged monitoring is more likely to result in an accurate diagnosis of atrial fibrillation patients than intermittent or short-term monitoring. In this study, we present an implantable ECG sensor to monitor atrial fibrillation patients in real time. The developed implantable sensor is composed of a micro controller unit, analog to digital converter, signal transmitter, antenna, and two electrodes. The sensor detects ECG signals from the two electrodes and transmits these signals to the external receiver that is carried by the patient. The sensor continuously transmits signals, so its battery consumption rate is extremely high. To overcome this problem, we consider using a wireless power transmission module in the sensor module. This module helps the sensor charge power wirelessly without holding the battery in the body. The size of the integrated sensor is approximately 0.12 inch x 1.18 inch x 0.19 inch. This sensor size is appropriate enough for cardiologists to insert the sensor into patients without the need for a major surgery. The data sampling rate was 300 samples/sec, and the frequency was 430 Hz for signal and power transmission. To verify the validation of the developed sensor, the small animal experiments were conducted.

scholarly journals Vascular Endothelial Cell Biology: An Update

2019 ◽  
Vol 20 (18) ◽  
pp. 4411 ◽  
Author(s):  
Krüger-Genge ◽  
Blocki ◽  
Franke ◽  
Jung
Keyword(s):  
Endothelial Cells ◽  
Cell Biology ◽  
Current Knowledge ◽  
Regional Blood Flow ◽  
Vascular Endothelial Cell ◽  
Inflammatory Cells ◽  
The Body ◽  
Arterial Tree ◽  
Foreign Materials ◽  
And Function

The vascular endothelium, a monolayer of endothelial cells (EC), constitutes the inner cellular lining of arteries, veins and capillaries and therefore is in direct contact with the components and cells of blood. The endothelium is not only a mere barrier between blood and tissues but also an endocrine organ. It actively controls the degree of vascular relaxation and constriction, and the extravasation of solutes, fluid, macromolecules and hormones, as well as that of platelets and blood cells. Through control of vascular tone, EC regulate the regional blood flow. They also direct inflammatory cells to foreign materials, areas in need of repair or defense against infections. In addition, EC are important in controlling blood fluidity, platelet adhesion and aggregation, leukocyte activation, adhesion, and transmigration. They also tightly keep the balance between coagulation and fibrinolysis and play a major role in the regulation of immune responses, inflammation and angiogenesis. To fulfill these different tasks, EC are heterogeneous and perform distinctly in the various organs and along the vascular tree. Important morphological, physiological and phenotypic differences between EC in the different parts of the arterial tree as well as between arteries and veins optimally support their specified functions in these vascular areas. This review updates the current knowledge about the morphology and function of endothelial cells, particularly their differences in different localizations around the body paying attention specifically to their different responses to physical, biochemical and environmental stimuli considering the different origins of the EC.

Bioethical Considerations on Cloning and Twinning

1997 ◽  
Vol 46 (3) ◽  
pp. 135-137 ◽  
Author(s):  
M. Milani-Comparetti
Keyword(s):  
Public Opinion ◽  
Scientific Community ◽  
The Body ◽  
Single Individual ◽  
Biological Processes ◽  
Deep Impact ◽  
Potential Applications ◽  
The Media ◽  
The Subject ◽  
Immortality Of The Soul

The subject of cloning has had a deep impact on both public opinion and the scientific community, asking themselves about its meaning, its possible extension to humans, its potential applications and implications.Cloning was often presented by the media as a technique that would allow perpetuating oneself.The resulting impact of cloning on public opinion might be interpreted, in part at least, as making real the dream of reincarnation.In the Christian faith cloning, as a hypothesis of reincarnation, has no place, since the soul is already immortal, while the body dies (excepting its reunion with its soul on the resurrection of the last day).Thus a person's immortality is a dogma of faith for the believer, but only as immortality of the soul, that will rejoin its body only at the end of earthly time, while in our “earthly time” the body is-mortal.The body's mortality is part of natural biological processes. Only in primitive organisms, such as bacteria, and in organisms reproducing through scions or similar processes (as farmers and florists well know) it is harder to set a definite moment for the birth or death of a single individual. But in sexually reproducing higher organisms, such as we are, the cycle of individual life is clearly encompassed and expressed by the well-known sequence whereby each individual “is born, grows, reproduces and dies”.If we consider the individual in all its manifestations – what we geneticists call the “phenotype”, resulting from the interactions between genotype and environment – each subject is undoubtedly endowed with his individuality.The repetition of the very same genotype does not mean repetition of the same individual, as clearly evidenced by the observation of identical twins (monozygotic, i.e., both derived from the same fertilized egg, the zygote) who, much as so closely resembling each other, are each endowed with his or her unique individuality.

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