scholarly journals Antimicrobial, antioxidant, anti-inflammatory and cytotoxic study of extracts of Guibourtia tessmanii (harms) J. Léonard from Gabon

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Cédric Sima Obiang ◽  
Rick Léonid Ngoua Meye Misso ◽  
Guy Roger Ndong Atome ◽  
Rany Berty Mbeng Obame ◽  
Joseph Privat Ondo ◽  
...  
Keyword(s):  
Biological Activities ◽  
Diffusion Method ◽  
Green Frog ◽  
Dpph Radical ◽  
Membrane Stabilization ◽  
Bactericidal Effects ◽  
Hemolysis Test ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Acetone Extracts

Abstract Background Gabonese flora abounds in a significant reserve of plants in medical matter. Thus, medicinal plants occupy a significant place in African pharmacopeia. Aim of this work was to evaluate the antimicrobial, antioxidant, anti-inflammatory and cytotoxic properties of extracts of Guibourtia tessmanii (Harms) J. Léonard. Methods The test for sensitivity to microorganisms was performed by the diffusion method, while the MICs and MBCs were evaluated by the microdilution technique. Antioxidant tests were performed by scavenging the DPPH and ABTS radicals. Anti-inflammatory activity was determined by protein denaturing and membrane stabilization methods. The cytotoxicity was evaluated on the tadpoles of the green frog. Results The antibacterial activity shows that the Gt F2 fraction and the water-acetone extract produced the greatest inhibitions. The water, water-ethanol and water-acetone extracts exhibited bactericidal effects on the majority of bacteria. In the case of trapping of the DPPH radical, the IC50 values varied from 6.92 ± 0.48 to 16.64 ± 0.20 μg/mL. For the decolouration of ABTS, oxidation was mainly inhibited by the water-acetetone, water-ethanol extracts and some fractions. The water and water-acetone extracts showed good inhibition of denaturation. The hemolysis test confirmed the good activities of the extracts. The lethal test showed that the LC50 drops from 171.37 ± 9.25 to 58.25 ± 7.21 μg/mL after 24 and 96 h of exposure. In tadpoles exposed to 7.81 μg / mL of extracts, the first mortalities (12.5%) were observed on the second day of exposure. From the ninth day, the mortality rate increased (25%) until the 16th day. Conclusion Our results show that Guibourtia tessmanii may be a promising product for the isolations of molecules responsible for biological activities.

scholarly journals Antibacterial, anti-inflammatory and antioxidant activities of Mahanintangtong and its constituent herbs, a formula used in Thai traditional medicine for treating pharyngitis

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Bhanuz Dechayont ◽  
Pathompong Phuaklee ◽  
Jitpisute Chunthorng-Orn ◽  
Thana Juckmeta ◽  
Onmanee Prajuabjinda ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Biological Activities ◽  
Ethanol Extract ◽  
Essential Medicines ◽  
Diffusion Method ◽  
Plant Materials ◽  
Tnf Α ◽  
Anti Inflammatory

Abstract Background Mahanintangtong is listed in the Thailand’s National List of Essential Medicines (NLEM). It is used to treat non-specific fevers and illnesses such as pharyngitis and chickenpox. In this study, we investigated the biological activities of the different medicinal plants used in the Mahanintangtong formula. Methods The plant materials were extracted by maceration and decoction. Antimicrobial activity, assessed by disc diffusion method, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) were compared with commercially available standard antibiotics. To elucidate the anti-inflammatory mechanisms, inhibition of nitric oxide (NO), tumor necrosis factor α (TNF-α), and interleukin 6 (IL-6) production was tested by Griess and ELISA techniques. Antioxidant activity was measured by ABTS and DPPH scavenging assays. Results The extracts with the best antimicrobial activities were carbonized Tectona grandis showing against Streptococcus pyogenes, Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. The ethanol extract of Dracaena loureiroi wood exhibited the highest NO and IL-6 inhibitory activity with IC50 values of 9.42 ± 1.81 and 12.02 ± 0.30 μg/mL, respectively. The ethanol extract of Pogostemon cablin had the highest TNF-α inhibitory with IC50 values of 10.68 ± 0.02 μg/mL. In anti-free radical testing, the ethanol extract of D. loureiroi displayed high antioxidant activity by both ABTS and DPPH assays. Conclusion The ethanol extracts from carbonized T. grandis and Mahanintangtong showed good antimicrobial activity, especially against S. pyogenes, and good anti-inflammatory activity. These findings are relevant to the pathogenesis of pharyngitis and justify additional studies to see if Mahanintangtong could have clinical utility.

scholarly journals DETERMINATION OF BIOLOGICAL ACTIVITIES OF THREE MARINE ALGAE COLLECTED FROM VISAKHAPATNAM COAST

2017 ◽  
Vol 10 (12) ◽  
pp. 274 ◽  
Author(s):  
Satya Lakshmi S
Keyword(s):  
Antioxidant Activity ◽  
Marine Algae ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Inflammatory Activity ◽  
Diffusion Method ◽  
Dpph Method ◽  
Anti Inflammatory

  Objective: Determination of biological activities of marine algae collected from the Visakhapatnam coastal region.Methods: Antibacterial activity of algal extracts determined by the well diffusion method, antioxidant activity was determined by reducing power (RP) method and 1, 1-diphenyl-2-picryl-hydrazil (DPPH) radical scavenging method finally anti-inflammatory activity was determined by human red blood cell stabilization method and egg albumin method.Results: Methanolic extracts of three algae have potential inhibition activity against Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus. Green alga Enteromorpha compressa has better antioxidant activity compared to the Gracilaria arcuata and Ulva fasciata when tested in RP and DPPH method. U. fasciata found good anti-inflammatory activity among the selected three algae.Conclusion: The three tested algae exhibited significant antibacterial and antioxidant activity compared to anti-inflammatory activity. These bioactive compounds containing macroalgae may find their commercial potential in medicine, food, and cosmetic industry

scholarly journals Antimicrobial and Anti-inflammatory properties of Juglans Regia Leaves

2019 ◽  
Vol 35 (6) ◽  
pp. 1756-1759
Author(s):  
Asma Eswayah ◽  
Nahla Labyad ◽  
Fadilah Aleanizy ◽  
Amal Belaid ◽  
Fulwah Alqahtani ◽  
...  
Keyword(s):  
Extraction Method ◽  
Inhibitory Concentration ◽  
Methanolic Extract ◽  
Juglans Regia ◽  
Antimicrobial Effect ◽  
Diffusion Method ◽  
Membrane Stabilization ◽  
Soxhlet Apparatus ◽  
Agar Diffusion Method ◽  
Anti Inflammatory

Antibacterial and anti-inflammatory activities of methanolic extract of Juglans regia L. leaves (Walnut) grown in Libya were investiagted. Hot extraction method by methanol using Soxhlet apparatus was utilized.The antimicrobial effect was studied using agar diffusion method. Human red blood cell membrane stabilization (HRBC) method was used to estimate the anti-inflammatory properties of the leave extract. The extract of leaves exhibited antimicrobial activity against Staphylococcus aureus, and Candida albicans with a minimum inhibitory concentration (MIC) of 25 and 50 mg/ml, respectively. In addition, the extract concentrations of 20, 40, and 100 μg/mL inhibited lysis of HRBC with values of 25.8%, 35.5%, and 70%, respectively. The results showed that the methanolic extract of Juglans regia leaves exhibited antibacterial activity against S. aureus, and antifungal activity against C. albican, and produced anti-inflammatory activity.

scholarly journals A Comparative Study on Phytochemical And Biological Activities of two Grewia Species

2016 ◽  
Vol 2 ◽  
pp. 53-60 ◽  
Author(s):  
Dharma Prasad Khanal ◽  
Bechan Raut ◽  
Manoj Kafle
Keyword(s):  
Medicinal Plants ◽  
Methanol Extract ◽  
Hexane Extract ◽  
Diffusion Method ◽  
Antibacterial Effects ◽  
Membrane Stabilization ◽  
Ground Sample ◽  
Rbc Membrane ◽  
Grewia Optiva ◽  
Anti Inflammatory

Background Medicinal plants are currently in considerable significance view due to their special attributes as a large source of therapeutic phytochemicals that may lead to the development of novel drugs. Herbs are staging a comeback and herbal ‘renaissance’ is happening all over the globe. The herbal products today symbolize safety in contrast to the synthetics that are regarded as unsafe to human and environment. It has been estimated that in developed countries such as United States, plant drugs constitute as much as 25% of the total drugs, while in fast developing countries such as China and India, the contribution is as much as 80%. Thus, the economic importance of medicinal plants is much more to countries such as Nepal than to rest of the world. Inventorisation of herbal drugs used in traditional and modern medicines for a country like Nepal, appears to be a stupendous task, where a number of well established indigenous or traditional systems, including Ayurveda, Unani, Siddha, Homoeopathy, Tibetan, Amchi, Yoga and Naturopathy are practiced along with modern medicine for the management of total health care system.Method Classic techniques for the solvent extraction of active constituents from medicinal plant matrices are based on the choice of solvent coupled with the use of heat. Soxhlet extraction is a general and well-established technique, which surpasses in performance other conventional extraction techniques. : In this method, finely ground sample of Grewia species were placed in a porous bag or “thimble” made from a strong filter paper or cellulose, which is place, is in thimble chamber of the Soxhlet apparatus. Extraction solvents was heated in the bottom flask, vaporizes into the sample thimble, condenses in the condenser and drip back. When the liquid content reaches the siphon arm, the liquid contents emptied into the bottom flask again and the process is continued. The resultant extracts were used for qualitative phytochemical analysis by color reactions, antibacterial effects by disc diffusion method and Human RBC membrane stabilization method was used to test the anti-inflammatory potential of the phytoconstituents.Findings Two species Grewia asiatica and Grewia optiva (family: Tiliaceae) from genus Grewia were studied for their phytochemical and biological properties. The result showed that the extractive value of Grewia optiva was found to be greater (2.56 % in hexane and 7.17 % in methanol) than Grewia asiatica (n-hexane 2.24% and methanol 5.04%) respectively. The test for the phytoconstituents for both species showed the presence of glycosides, steroids, tannins, flavonoids, terpenoids and saponins. No any extract of both species at dose of (0.25mg, 0.5mg and 1mg per cup) possessed a zone of inhibition against tested bacterial strains. The potency of n-hexane extract and methanol extract of both species for Human RBC membrane stabilization test were compared with standard Diclofenac potassium. HRBC stabilization and resulted a good membrane stabilization of n-hexane extract of Grewia asiatica (40.89%, 73.57%, 78.23%, 80.07%, 80.91% and 51.95%) at (50 μg/ml, 100μg/ml, 200μg/ml, 400μg/ml, 600μg/ml and 800μg/ml) than methanol extract of both species. The result of the both species possessed similar phytochemicals and showed some membrane stabilization property which supports the traditional use.Conclusion Alkaloids were absent in the Grewia species extracts. The tested extracts have no any antibacterial effects against the tested bacterial stamps. The human RBC membrane stabilization tests were compared with standard Diclofenac potassium showed the good membrane stabilization of n-hexane extract of Grewia asiatica indicating the potent anti-inflammatory effect.Journal of Manmohan Memorial Institute of Health Sciences Vol. 2 2016 p.53-60

scholarly journals Study on Biological Activity of Litsea monopetala From Panchthar District of Nepal

2020 ◽  
Vol 25 (2) ◽  
pp. 113-118
Author(s):  
Subash Khanal ◽  
Bishan Datt Bhatt
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Bioactive Substances ◽  
Ic50 Values ◽  
Acetone Extracts ◽  
Dpph Radical Scavenging

The Panchthar, a hilly district of Eastern Nepal, has several medicinal plants which are used by indegenous people for the treatments of different diseases. In the present work, phytochemical and biological activities of hexane, ethyl acetate, acetone, and methanol extracts of Litsea monopetala leaves from the Pachthar district were analyzed. The phytochemical screening demonstrated the presence of major secondary metabolites like flavonoids, alkaloids, terpenoids, polyphenols, glycosides, and proteins. The antibacterial activity was estimated by the disc diffusion method against Bacillus substilus and Escherichia coli. The result showed that ethyl acetate extract exhibit the highest zone of inhibition (ZOI) value of 18 mm/disc. Acetone extract also showed significant antibacterial activity at the concentration of 100 mg/mL. Antioxidant activity from DPPH radical scavenging assay displayed the inhibitory concentration (IC50) values of dried methanol and acetone extracts were 30.74 μg/mL and 202.86 μg/mL, respectively. The methanol extract has the highest total phenolic content of 360.14 mg GAE/g extract and the highest total flavonoid content of 27.13 mg QE/g extract. The present study revealed that Litsea monopetala plant from the Panchthar district is a source of several bioactive substances and can be used in several drugs formulation.

scholarly journals In-vitro Anti-lipoxygenase and Membrane Stabilization Activity of Crude Extracts of Euphorbia lateriflora (Schum and Thonn)

2020 ◽  
pp. 24-32
Author(s):  
Olasunkanmi Adedoyin A. ◽  
Fadahunsi Olumide Samuel ◽  
Olorunnisola Olubukola Sinbad
Keyword(s):  
Lipoxygenase Activity ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Red Cell Membrane ◽  
Crude Extracts ◽  
University Of Technology ◽  
Comparable Activity ◽  
Anti Inflammatory ◽  
Ethanol Extracts

Aims: This study was aimed at evaluating the in-vitro anti-inflammatory potential of crude extracts of Euphorbia lateriflora.  Place of Study: Department of Biochemistry, Ladoke Akintola University of Technology, Ogbomoso, Nigeria. Methodology: The in-vitro anti-inflammatory activity of aqueous and ethanol extracts of E. lateriflora were evaluated via anti-lipoxygenase and red cell membrane stabilization assays using spectrophotometric technique. Results: The aqueous and ethanol extracts demonstrated significant anti-lipoxygenase activity with the aqueous extracts (IC50: 0.34 mg/ml) and the ethanol extracts (IC50: 0.25 mg/ml), showing comparable activity with standard indomethacin (0.27 mg/ml). Contrary to what was observed in the anti-lipoxygenase study, the aqueous extract demonstrated a better membrane stabilization activity with IC50 (1.13 mg/ml) than the ethanol extracts (5.44 mg/ml). The standard diclofenac drug had an IC50 of 1.02 mg/kg. Both the aqueous and the ethanol extracts demonstrated significant anti-inflammatory activity. Conclusion: Observation from this study established the considerable anti-inflammatory potential of this plant by inhibiting lipoxygenase activity and stabilization the membrane of the red blood cells.

scholarly journals Phytochemical and Pharmacological Investigations of Uraria lagopodies DC. and Urena lobata L.

2012 ◽  
Vol 11 (1) ◽  
pp. 65-69
Author(s):  
Md Torequl Islam ◽  
Mohammed Ibrahim ◽  
Md Qamrul Ahsan ◽  
M Mohi Uddin Chowdhury ◽  
Md Aslam Hossain ◽  
...  
Keyword(s):  
Body Weight ◽  
Reducing Sugars ◽  
Ethanol Extract ◽  
Membrane Stabilization ◽  
Pharmacological Activities ◽  
Herbal Preparations ◽  
Activity Test ◽  
Anti Inflammatory ◽  
Ethanol Extracts ◽  
Dose Dependent

Easy availability and reduced side effects of plants and plant-derived herbal preparations are the gifts of nature and are useful since ancient time for the treatment of various diseases. From this view point twoBangladeshi plants, Uraria lagopodies DC. and Urena lobata L. have been evaluated for their possible pharmacological activities. Preliminary phytochemical screenings with the crude ethanol extracts demonstrated the  presence of alkaloids, glycosides, tannins, saponins, steroids and reducing sugars. The crude extract of U. lagopodies significantly (p<0.001) increased the charcoal evacuation by 375 min at 500 mg/kg body weight of mice when  compared to the standard, Loperamide by 391.8 min at 5 mg/kg b.w. In the acetic acid induced analgesic activity test,  the oral administration of ethanol extract of U. lobata produced significant (p<0.05) pain relieving activity (number  of writhing 32) at 500 mg/kg body weight as compared to standard diclofenac-Na. Both the crude extracts showed mild anti-inflammatory and membrane stabilizing activities during the heat induced anti-inflammatory test and  membrane stabilization screening. A dose dependent biological activity was observed during the study period. DOI: http://dx.doi.org/10.3329/dujps.v11i1.12490 Dhaka Univ. J. Pharm. Sci. 11(1): 65-69, 2012 (June)

scholarly journals Comparison of Two Ginkgo biloba L. Extracts on Oxidative Stress and Inflammation Markers in Human Endothelial Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Stefano Piazza ◽  
Barbara Pacchetti ◽  
Marco Fumagalli ◽  
Fabrizia Bonacina ◽  
Mario Dell’Agli ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Ginkgo Biloba ◽  
Vascular Endothelial Cells ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Beneficial Effects ◽  
Proinflammatory Mediators ◽  
Ethanol Extracts ◽  
Acetone Extracts

Atherosclerosis is characterized by interaction between immune and vascular endothelial cells which is mediated by adhesion molecules occurring on the surface of the vascular endothelium leading to massive release of proinflammatory mediators. Ginkgo biloba L. (Ginkgoaceae) standardized extracts showing beneficial effects are commonly prepared by solvent extraction, and acetone is used according to the European Pharmacopoeia recommendations; the well-known Ginkgo biloba acetone extract EGb761® is the most clinically investigated. However, in some countries, the allowed amount of solvent is limited to ethanol, thus implying that the usage of a standardized Ginkgo biloba ethanol extract may be preferred in all those cases, such as for food supplements. The present paper investigates if ethanol and acetone extracts, with comparable standardization, may be considered comparable in terms of biological activity, focusing on the radical scavenging and anti-inflammatory activities. Both the extracts showed high inhibition of TNFα-induced VCAM-1 release (41.1-43.9 μg/mL), which was partly due to the NF-κB pathway impairment. Besides ROS decrease, cAMP increase following treatment with ginkgo extracts was addressed and proposed as further molecular mechanism responsible for the inhibition of endothelial E-selectin. No statistical difference was observed between the extracts. The present study demonstrates for the first time that ethanol and acetone extracts show comparable biological activities in human endothelial cell, thus providing new insights into the usage of ethanol extracts in those countries where restrictions in amount of acetone are present.

scholarly journals Total Phenolics, Antioxidative and Antimicrobial Activities of Some Edible Ascomycota Collected from Niğde

2021 ◽  
Vol 9 (10) ◽  
pp. 1915-1920
Author(s):  
Şükrü Canpolat ◽  
Ilgaz Akata ◽  
Yasemin İşlek ◽  
Elif Canpolat ◽  
Cemil İşlek
Keyword(s):  
Antimicrobial Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Total Phenolic ◽  
Dpph Radical ◽  
Morchella Esculenta ◽  
Ethanol Extracts ◽  
Dpph Scavenging ◽  
Dpph Radical Scavenging

Mushrooms have been used as food and medicinal purposes for centuries. In this study, some edible Ascomycota were collected in 2018 and 2019 from Niğde, Turkey. Molecular identifications of the samples were performed afterward. PCR products were sent for sequence analysis after ITS fragments were amplified with suitable primers concerning DNA isolation of samples. The obtained data were compared with the Genbank database for samples’ determination. The mushroom samples were determined to be Terfezia claveryi Chatin and Morchella esculenta (L.) Pers. Samples were extracted by ethanol and methanol to determine antimicrobial activity using the disc diffusion method. Antimicrobial zones against microorganisms were measured. Then DPPH radical scavenging effects and total phenolic content of T. claveryi and M. esculenta mushroom samples were studied. The highest antimicrobial activity was observed in ethanol extracts of M. esculenta. When the DPPH radical scavenging activities are taken into account, the activity is increased depending on the concentration. The highest DPPH scavenging activity was found in the extract of M. esculenta with 40.86%. It was determined that the total phenolic substances in methanol extracts of mushroom samples varies between 533.28 ± 1,15-537.34 ±2.20 μg GAE / mg extract.

scholarly journals In-vitro Antioxidant and anti-inflammatory activities valorisation of tannin crude extract of Helianthemum helianthemoïdes (Desf.) Grosser

2020 ◽  
Vol 10 (1-s) ◽  
pp. 135-139
Author(s):  
Nouioua Wafa ◽  
Gaamoune Sofiane
Keyword(s):  
Crude Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dpph Radical ◽  
Stabilization Method ◽  
Membrane Stabilization ◽  
Inflammatory Agent ◽  
Anti Inflammatory ◽  
Dpph Radical Scavenging

Plants have always been used by humans to relieve and cure many diseases unfortunately the majority of them still unknown. The aims of this study were to evaluate the antioxidant and anti-inflammatory properties of tannin crude extract of Helianthemum helianthemoïdes (Desf.) Grosser. The antioxidant activity was performed by DPPH radical scavenging method and the reducing power essay; however, the anti-inflammatory activity was tested with the Human Red Blood Cell (HRBC) membrane stabilization method. The obtained results indicated high antioxidant potential and a perfect anti-inflammatory agent. Keywords: Helianthemum helianthemoïdes;  DPPH; reducing power; HRBC

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