Effect of chronic neurogenic pain on blood levels of hormones in patients with melanoma depending on their gender.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e22097-e22097
Author(s):  
Viktoria V. Pozdnyakova ◽  
Elena M. Frantsiyants ◽  
Valeria A. Bandovkina ◽  
Ludmila Ya. Rozenko ◽  
Maria G. Ilchenko ◽  
...  
Keyword(s):  
Cutaneous Melanoma ◽  
Specific Effect ◽  
Control Group ◽  
Blood Levels ◽  
Disease Course ◽  
Comorbid Disease ◽  
Neurogenic Pain ◽  
Male And Female ◽  
Melanoma Patients ◽  
Disease Stages

e22097 Background: Chronic neurogenic pain (CNP) as a comorbid disease is quite frequent, but its effect on malignant diseases is poorly studied. The risk of cutaneous melanoma in women is higher, but the disease course in men is more severe. The purpose of the study was to reveal the effect of CNP on the levels of sex hormones in the blood of patients with melanoma depending on their gender. Methods: Blood levels of estradiol (E2), estrone (E1), progesterone (P4), testosterone (T) and prolactin (PRL) were measured by ELISA in patients with Т3-4NxM0 melanoma (M): 21 women with CNP (pelvic pain - 7, osteochondrosis – 14), mean age 67.2±2.7 years; 17 men with CNP (osteochondrosis), mean age 65.6±3.1 years. The control group included patients with melanoma similar in age, gender and disease stages without CNP. Results: In women with M+CNP, E2 and P4 were decreased by 1.8 times, while E1 was 1.4 times, T – 2 times, PRL – 1.5 times higher, compared to controls. In men with M+CNP, blood levels of estrogens were unchanged, PRL was 2.5 times lower and P4 and T – 1.3 times higher, compared to the corresponding control levels. Conclusions: CNP upregulated blood levels of androgens in both male and female melanoma patients, and caused the inversion of estrogens only in women with the prevalence of E1 over E2. Gender differences in the CNP influence included the elevation of progesterone and a decrease of prolactin in the blood of men, and P4 deficiency with increased prolactin in women with M+CNP. The specific effect of CNP on the hormonal profile in patients with cutaneous melanoma should be considered in choosing antitumor treatment.

Chronic neurogenic pain activates growth factors in the blood of patients with cutaneous melanoma.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e22082-e22082
Author(s):  
Valeria A. Bandovkina ◽  
Elena M. Frantsiyants ◽  
Ludmila Ya. Rozenko ◽  
Viktoria V. Pozdnyakova ◽  
Natalia D. Cheryarina ◽  
...  
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factor ◽  
Cutaneous Melanoma ◽  
Control Group ◽  
Blood Levels ◽  
Vascular Endothelial ◽  
Neurogenic Pain ◽  
Mesenchymal Transition ◽  
Endothelial Growth Factor ◽  
Tgf Β1

e22082 Background: Clusters of tumor cells in blood vessels secrete VEGF family factors necessary for the formation of premetastatic niches. TGF-β activates the epidermal-mesenchymal transition (EMT). The purpose of the study was to analyze levels of angiogenesis and EMT factors in the blood of patients with cutaneous melanoma (M) and chronic neurogenic pain (CNP). Methods: Blood levels of VEGF-A, VEGF-C, VEGFR-1, VEGFR-3, TGF-β1 and TGF-βR2 were measured by ELISA in patients with Т3-4NxM0 melanoma: 21 women with CNP (pelvic pain - 7, osteochondrosis – 14), mean age 67.2±2.7 years; 17 men with CNP (osteochondrosis), mean age 65.6±3.1 years. The control group included patients with melanoma similar in age, gender and disease stages without CNP. Results: VEGF-A in women and men with M+CNP was higher than in controls by 2.7 and 24.9 times respectively; VEGFR-1 was decreased in women by 1.8 times and increased in men by 1.8 times. VEGF-С was unchanged in women and 1.5 times higher in men, and VEGFR-3 was increased by 2.2 times in women and unchanged in men. TGF-β1 was elevated in women and men with M+CNP by 1.4 and 1.8 times, compared to controls, TGF-βR2 – by 1.9 and 2 times respectively. Conclusions: In the blood of women with M+CNP, CNP activates the blood vascular endothelial growth factor VEGF-A and the factor of epidermal-mesenchymal transition TGF-β, while in the blood of men with M+CNP it activates the blood vascular endothelial growth factor VEGF-A, the lymphatic vascular endothelial growth factor VEGF-C and the factor of epidermal-mesenchymal transition TGF-β. VEGF mediates vascular permeability and is associated with the mobilization of endothelial progenitor cells from the bone marrow into premetastatic niches. Activation of TGF-β signaling promotes the induction of the epidermal-mesenchymal transition and supports the properties of cancer stem cells.

Relationship of chronic neurogenic pain and fibrinolysis in the blood of patients with melanoma.

2020 ◽  
Vol 38 (15_suppl) ◽  
pp. e22085-e22085
Author(s):  
Ludmila Ya. Rozenko ◽  
Elena M. Frantsiyants ◽  
Valeria A. Bandovkina ◽  
Yulia A. Pogorelova ◽  
Natalia D. Cheryarina ◽  
...  
Keyword(s):  
Cutaneous Melanoma ◽  
Special Treatment ◽  
Regulation System ◽  
Control Group ◽  
Blood Levels ◽  
Neurogenic Pain ◽  
Additional Information ◽  
Plasminogen Activation System ◽  
Relationship Of ◽  
Pai 1

e22085 Background: The plasminogen regulation system is critical in triggering proteolysis to cleave the extracellular matrix and basement membrane. The “uPA-uPAR” system is involved in extravascular fibrinolysis and is responsible for the formation of plasmin associated with invasion and metastasis. The purpose of the study was to analyze levels of factors of the plasminogen regulation system in the blood of patients with cutaneous melanoma (M) and chronic neurogenic pain (CNP). Methods: Blood levels of PAP, uPA, uPAR and PAI-1 were measured by ELISA in patients with Т3-4NxM0 melanoma: 21 women with CNP (pelvic pain - 7, osteochondrosis – 14), mean age 67.2±2.7 years; 17 men with CNP (osteochondrosis), mean age 65.6±3.1 years. The control group included patients with melanoma similar in age, gender and disease stages without CNP. Results: Blood levels of PAP in women with M+CNP were twice higher than in patients with M without CNP, the levels in men with M+CNP did not change; uPA content increased in both women and men with M without CNP by 1.4 and 1.5 times, uPA activity was elevated only in women by 2.4 times, in men it was reduced by 2 times; uPAR was increased by 1.5 times in women and men compared to the values in both female and male patients with M without CNP. PAI-1 content in women and men with M+CNP was similar to control values, and its activity was increased in women by 1.5 times and decreased in men by 2.5 times. Conclusions: The study gives significant additional information on the effect of CNP on relationships in the plasminogen activation system in a combination of cutaneous melanoma and CNB showing marked gender differences. The results should be taken into account in the special treatment of patients with melanoma.

scholarly journals Serum level of vitamin D3 in cutaneous melanoma

2014 ◽  
Vol 12 (4) ◽  
pp. 473-476 ◽  
Author(s):  
Renato Santos de Oliveira Filho ◽  
Daniel Arcuschin de Oliveira ◽  
Vitor Augusto Melão Martinho ◽  
Célia Beatriz Gianotti Antoneli ◽  
Ludmilla Altino de Lima Marcussi ◽  
...  
Keyword(s):  
Vitamin D ◽  
Reference Values ◽  
Vitamin D3 ◽  
Cutaneous Melanoma ◽  
Reference Level ◽  
Control Group ◽  
Similar Distribution ◽  
General Group ◽  
Melanoma Patients ◽  
Active Disease

Objective To compare the level of vitamin D3 in cutaneous melanoma patients, with or without disease activity, with reference values and with patients from a general hospital.Methods The serum levels of vitamin D3 were measured in cutaneous melanoma patients, aged 20 to 88 years, both genders, from January 2010 to December 2013. The samples from the general group were processed at Hospital Israelita Albert Einstein (control group). Data analysis was performed using the Statistics software.Results A total of 100 patients were studied, 54 of them men, with mean age of 54.67 years, and 95 Caucasian. Out of these 100 patients, 17 had active disease. The average levels of vitamin D3 in the melanoma patients were lower than the level considered sufficient, but above the average of the control group. Both groups (with or without active disease) of patients showed a similar distribution of vitamin D3 deficiency.Conclusion Vitamin D3 levels in melanoma patients were higher than those of general patients and lower than the reference level. If the reference values are appropriate, a large part of the population had insufficient levels of vitamin D, including those with melanoma, or else, this standard needs to be reevaluated. No difference in vitamin D3 levels was found among melanoma patients with or without active disease. More comprehensive research is needed to assess the relation between vitamin D and melanoma.

scholarly journals A Training Proposal to Improve Multidirectional Running Technique in Male and Female Handball Players: A Pilot Study

2021 ◽  
Vol 18 (4) ◽  
pp. 2056
Author(s):  
Carmen Ferragut ◽  
Román Pedreira ◽  
José Julio Espina ◽  
Helena Vila
Keyword(s):  
Team Sports ◽  
T Test ◽  
Control Group ◽  
Sprint Training ◽  
Countermovement Jump ◽  
Squat Jump ◽  
Male And Female ◽  
Intervention Protocol ◽  
Post Test ◽  
Experimental Group

Multidirectional running has been described as an important factor in team sports performance. The aim of the present study was to determine changes in T-test, 505 time, 10 m sprint, squat jump (SJ), countermovement jump (CMJ), countermovement jump right leg (CMJRL), and countermovement jump left leg (CMJLL) following exposure to 12 sessions over 4 weeks of a multidirectional running sprint training intervention in male and female handball players. A total of 31 handball players (15 male and 16 female) were recruited for this study and then randomly assigned to an experimental group (EG) or control group (CG). Male EG players showed improvements in 505 Preferred Side (PS) (p ≤ 0.05), 505 Non-Preferred Side (NPS) (p ≤ 0.05), and 10 m sprint (p ≤ 0.05), while female EG players presented statistically significant improvements between pre- and post-test for the T-test (p ≤ 0.05), 505 PS (p ≤ 0.05), 505 NPS (p ≤ 0.05), and 10 m sprint (p ≤ 0.05). No statistically significant pre- and post-test differences were observed in CG (all p ≥ 0.05) or between male and female players. We found an improvement in handball players’ agility and speed of movement following the intervention protocol, suggesting the need to introduce this program into our training sessions. It may also be necessary to select and develop more specific tests in order to evaluate multidirectional work in handball players.

scholarly journals Study protocol: randomised controlled trial evaluating exercise therapy as a supplemental treatment strategy in early multiple sclerosis: the Early Multiple Sclerosis Exercise Study (EMSES)

BMJ Open ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. e043699
Author(s):  
Morten Riemenschneider ◽  
Lars G Hvid ◽  
Steffen Ringgaard ◽  
Mikkel K E Nygaard ◽  
Simon F Eskildsen ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Randomised Controlled Trial ◽  
Usual Care ◽  
Treatment Strategy ◽  
Controlled Trial ◽  
Treatment Strategies ◽  
Control Group ◽  
Disease Course ◽  
Disability Progression ◽  
Randomised Controlled

IntroductionIn the relapsing remitting type of multiple sclerosis (MS) reducing relapses and neurodegeneration is crucial in halting the long-term impact of the disease. Medical disease-modifying treatments have proven effective, especially when introduced early in the disease course. However, patients still experience disease activity and disability progression, and therefore, supplemental early treatment strategies are warranted. Exercise appear to be one of the most promising supplemental treatment strategies, but a somewhat overlooked ‘window of opportunity’ exist early in the disease course. The objective of this study is to investigate exercise as a supplementary treatment strategy early in the disease course of MS.Methods and analysisThe presented Early Multiple Sclerosis Exercise Study is a 48-week (plus 1-year follow-up) national multicentre single-blinded parallel group randomised controlled trial comparing two groups receiving usual care plus supervised high-intense exercise or plus health education (active control). Additionally, data will be compared with a population-based control group receiving usual care only obtained from the Danish MS Registry. The primary outcomes are annual relapse rate and MRI derived global brain atrophy. The secondary outcomes are disability progression, physical and cognitive function, MS-related symptoms, and exploratory MRI outcomes. All analyses will be performed as intention to treat.Ethics and disseminationThe study is approved by The Central Denmark Region Committees on Health Research Ethics (1-10-72-388-17) and registered at the Danish Data Protection Agency (2016-051-000001 (706)). All study findings will be published in scientific peer-reviewed journals and presented at relevant scientific conferences.Trial registration numberNCT03322761.

scholarly journals Local and systemic inflammation after implantation of a novel iron based porous degradable bone replacement material in sheep model

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Bernd Wegener ◽  
Maik Behnke ◽  
Stefan Milz ◽  
Volkmar Jansson ◽  
Christian Redlich ◽  
...  
Keyword(s):  
Mechanical Stability ◽  
Bone Substitutes ◽  
Histological Evaluation ◽  
Control Group ◽  
Blood Levels ◽  
Porous Iron ◽  
Sheep Model ◽  
Bone Replacement ◽  
Alloy Particles ◽  
Iron Based

AbstractDespite the high potential of healthy bone to regenerate, the reconstruction of large bone defects remains a challenge. Due to the lack of mechanical stability of existing bone substitutes, recently developed degradable metallic alloys are an interesting alternative providing higher load-bearing capabilities. Degradable iron-based alloys therefore might be an attractive innovation. To test the suitability of a newly-designed iron-based alloy for such applications, an animal experiment was performed. Porous iron-based degradable implants with two different densities and a control group were tested. The implants were positioned in the proximal tibia of Merino sheep. Over a period of 6 and 12 months, blood and histological parameters were monitored for signs of inflammation and degradation. In the histological evaluation of the implants` environment we found degraded alloy particles, but no inflammatory reaction. Iron particles were also found within the popliteal lymph nodes on both sides. The serum blood levels of phosphorus, iron and ferritin in the long term groups were elevated. Other parameters did not show any changes. Iron-based degradable porous bone replacement implants showed a good biocompatibility in this experiment. For a clinical application, however, the rate of degradation would have to be significantly increased. Biocompatibility would then have to be re-evaluated.

Increased S100B Levels in Cannabis Use Disorder

2015 ◽  
Vol 22 (4) ◽  
pp. 177-180 ◽  
Author(s):  
Huseyin Bayazit ◽  
Erdinc Cicek ◽  
Salih Selek ◽  
Nurten Aksoy ◽  
I. Fatih Karababa ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Neuronal Damage ◽  
Cannabis Use ◽  
S100b Protein ◽  
Control Group ◽  
Blood Levels ◽  
Cannabis Use Disorder ◽  
Protein Levels ◽  
S100 Calcium Binding Protein ◽  
The Mean

Background: It has been determined that cannabis has adverse effects on brain tissue, and that increased S100 calcium binding protein B (S100B) blood levels are markers of neuronal damage. Therefore, the aim of this study was to evaluate the S100B levels in cannabis use disorder. Method: Thirty-two patients with cannabis use disorder and 31 matched healthy controls were enrolled in this study. Appropriate blood samples were taken from the enrolled subjects, and the serum S100B protein levels were measured with an electrochemiluminescence immunoassay for the quantification of the protein. Findings: We found significantly increased S100B protein levels in patients with cannabis use disorder. The mean serum concentration of S100B was 0.081 ± 0.018 μg/l in patients with cannabis use disorder, and 0.069 ± 0.018 μg/l in the control group (p = 0.008). Interpretation: Our data suggest that elevated S100B protein levels might indicate neuronal damage in the brains of people with cannabis use disorder.

scholarly journals Kidney Protection by Hypothermic Total Liquid Ventilation after Cardiac Arrest in Rabbits

2014 ◽  
Vol 120 (4) ◽  
pp. 861-869 ◽  
Author(s):  
Renaud Tissier ◽  
Sebastien Giraud ◽  
Nathalie Quellard ◽  
Béatrice Fernandez ◽  
Fanny Lidouren ◽  
...  
Keyword(s):  
Cardiac Arrest ◽  
Life Support ◽  
Kidney Injury ◽  
Interferon Γ ◽  
Control Group ◽  
Blood Levels ◽  
Toll Like Receptor ◽  
Liquid Ventilation ◽  
Monocyte Chemoattractant Protein 1 ◽  
Group 2

Abstract Background: Total liquid ventilation (TLV) with perfluorocarbons has been shown to induce rapid protective cooling in animal models of myocardial ischemia and cardiac arrest, with improved neurological and cardiovascular outcomes after resuscitation. In this study, the authors hypothesized that hypothermic TLV can also limit kidney injury after cardiac arrest. Methods: Anesthetized rabbits were submitted to 15 min of untreated ventricular fibrillation. After resuscitation, three groups of eight rabbits each were studied such as (1) life support plus hypothermia (32°–33°C) induced by cold TLV (TLV group), (2) life support without hypothermia (control group), and (3) Sham group (no cardiac arrest). Life support was continued for 6 h before euthanasia and kidney removal. Results: Time to target esophageal temperature was less than 5 min in the TLV group. Hypothermia was accompanied by preserved renal function in the TLV group as compared with control group regarding numerous markers including creatinine blood levels (12 ± 1 vs. 16 ± 2 mg/l, respectively; mean ± SEM), urinary N-acetyl-β-(d)-glucosaminidase (1.70 ± 0.11 vs. 3.07 ± 0.10 U/mol of creatinine), γ-glutamyltransferase (8.36 ± 0.29 vs. 12.96 ± 0.44 U/mol of creatinine), or β2-microglobulin (0.44 ± 0.01 vs. 1.12 ± 0.04 U/mol of creatinine). Kidney lesions evaluated by electron microscopy and conventional histology were also attenuated in TLV versus control groups. The renal-protective effect of TLV was not related to differences in delayed inflammatory or immune renal responses because transcriptions of, for example, interferon-γ, tumor necrosis factor-α, interleukin-1β, monocyte chemoattractant protein-1, toll-like receptor-2, toll-like receptor-4, and vascular endothelial growth factor were similarly altered in TLV and control versus Sham. Conclusion: Ultrafast cooling with TLV is renal protective after cardiac arrest and resuscitation, which could increase kidney availability for organ donation.

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