scholarly journals Antioxidant effect of ethanolic extract of Pleurotus Ostreatus on 4-hydroxy-2-nonenal (HNE) and glutathione (GSH) level in lung rats exposed to cigarette smoke

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 1234
Author(s):  
Santun Bhekti Rahimah ◽  
Arto Yuwono Soeroto ◽  
Diah Dhianawaty Djunaedi ◽  
Tatang Bisri
Keyword(s):  
Cigarette Smoke ◽  
Pleurotus Ostreatus ◽  
Rank Correlation ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Group Iv ◽  
Male Rats ◽  
Group Iii ◽  
Endogenous Antioxidant ◽  
One Way Anova

Background: Cigarette smoke can trigger oxidative stress. An alternative to overcome the harmful effects of cigarette smoke is through antioxidants. White oyster mushrooms (Pleurotus Ostreatus) are a source of exogenous antioxidants because many contain active compounds for potential antioxidants such as phenol and flavonoid compounds. The purpose of this study was to analyze the effect of ethanolic extract of Pleurotus ostreatus on 4-hydroxy-2-nonenal (HNE) and glutathione (GSH), as well as to analyze their correlation in the lung of Wistar male rats exposed to cigarette smoke. Methods: The study was a preclinical experiment conducted on 24 rats divided into four groups. The treatment was carried out for 42 days and antioxidant effects were assessed through levels of HNE and GSH in rat lungs. Groups were divided as follows: I- normal control, II- negative control, III and IV exposed to cigarette smoke for 60 minutes/day. Group III (treatment group) was treated with ethanolic extract of Pleurotus ostreatus at 250 mg/kg BW rat/day, and group IV (comparison group) was treated with N-acetyl cysteine 600 mg /day. Data analysis used was one-way ANOVA and Kruskal Wallis test, and Spearmen rank correlation coefficient test. Results: The results showed that the group receiving ethanolic extract of Pleurotus ostreatus had HNE levels of (44,18 ± 2,09 pg/mL) and GSH (0,04 ± 0,00 pg/mL) protein. This extract significantly increased GSH levels and inhibited the increase of HNE levels. Results of GSH (p≤0.01) showed significant results using one-way ANOVA. Conclusions: The ethanolic extract of P. ostreatus can prevent lipid peroxidation and decrease endogenous antioxidant levels in lung cells exposed to cigarette smoke. Ethanolic extract of Pleurotus ostreatus has good antioxidant potential.

scholarly journals Immunomodulatory Activity of Xestospongia sp. Ethanolic Extract Towards Interferon-gamma (IFN- γ) and Tumor Necrosis Factor-alpha (TNF-α) Levels in Wistar Male Rats

2020 ◽  
Vol 6 (2) ◽  
Author(s):  
Adryan Fristiohady ◽  
Jumadil ◽  
Wahyuni ◽  
Muh. Hajrul Malaka ◽  
Wa Ode Harnita ◽  
...  
Keyword(s):  
Ethanolic Extract ◽  
Group Iv ◽  
Male Rats ◽  
Immunomodulatory Activity ◽  
Necrosis Factor Alpha ◽  
Group Iii ◽  
Tnf Α ◽  
Factor Alpha ◽  
Ifn Γ ◽  
Necrosis Factor

Xestospongia sp. is one of marine sponge belongs to demosponges class that mainly found in Southeast Sulawesi and the secondary metabolites contained in Xestospongia sp. suspected to have immunomodulatory activity. A previous study exhibited the immunomodulatory of Xestospongia sp. ethanolic extract (XEE) at dose of 300 and 400 mg/Kg BW by affecting the phagocytic activity of macrophages. Thus, this study aims to investigate the effect of XEE towards interferon-gamma (IFN-γ) and tumor necrosis factor-alpha (TNF-α) at dose of 300 and 400 mg/Kg BW. Wistar male rats are divided into 4 groups (n=6) randomly and treated for 7 days orally each as follow: group I (XEE dose of 300 mg/KgBW); group II (XEE dose of 400 mg/KgBW); group III (0.5% NaCMC); and group IV (commercial phylantii extract). On day 8, animals were infected with Staphylococcus aureus and left for 1 hour. Animals were sacrificed and the blood was drawn by cardiac puncture (3 mL), followed by analyzed under ELISA Kit for IFN-γ and TNF-α. Collected data were analyzed statistically using SPSS®. The IFN-γ levels obtained were 350.113; 392.970; 118.416; and 61.958 ρg/mL, respectively and the TNF-α were 2808; 1308; 778; and 845.5 ρg/mL, respectively. According to results obtained, both doses of XEE are affecting the IFN-γ and TNF-α levels (p<0.05) compared to group III as negative control, and group IV as positive control. As conclusion, XEE of both doses is increasing IFN-γ and TNF-α levels of animals that respond to phagocytic activity

scholarly journals Immunomodulator Activity of Effervescent Granule of Wualae Fruit (Etlingera elatior (Jack) R.M. Smith) Based on Specific Phagocytic Activity

2019 ◽  
Vol 2 (2) ◽  
pp. 35-40 ◽  
Author(s):  
Adryan Fristiohady ◽  
Wa Ode Siti Zubaydah ◽  
Wahyuni Wahyuni ◽  
Mirda Mirda ◽  
Saripuddin Saripuddin ◽  
...  
Keyword(s):  
Phagocytic Activity ◽  
Ethanolic Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Etlingera Elatior

The previous study reported that Etlingera elatior (Wualae) has activity as immunostimulant with dose at 300 mg/kg BW and 400 mg/kg BW. Formulating natural product into effervescent granule (GE) can increase practicality and interest to consume traditional medicine. This study aims to investigate the immunostimulant activity of Wualae ethanolic extract in the effervescent granule. Wualae was macerated with ethanol then formulated into effervescent granule at dose 300 mg/kgBW and 400 mg/kgBW. Formulations were evaluated. Animals were divided into 4 groups and treated with: group I (positive control); group II (negative control); group III (GE dose at 300mg/kgBW); and group IV (GE at dose 400mg/kgBW) for 7 days and at days 8, groups were infected by Staphylococcus aureus intraperitoneally. Immunostimulant activity was measured by calculating the Specific Phagocytic Activity (SPA) of macrophage. Data collected from the evaluation and measurement of the SPA then analyzed using SPSS. Results from the evaluation were considered good except the moisture content of effervescent granule. Results from SPA was found that effervescent granule at dose 300 mg/kgBW and at dose 400 mg/kgBW have 26.33% and 32.5% consecutively with significance 0.02 (p<0.05). Thus, effervescent granule has comparable phagocytosis activity with positive control.

scholarly journals PENGARUH EKSTRAK ETANOLIK BUAH PARIJOTO (Medinilla speciosa Blume) TERHADAP KADAR GULA DARAH DAN FUNGSI SEKSUAL TIKUS JANTAN GALUR WISTAR MODEL DIABETES MELLITUS KRONIK

2018 ◽  
Vol 15 (2) ◽  
pp. 1
Author(s):  
Rina Wijayanti ◽  
Asih Puji Lestari
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Sexual Function ◽  
Glucose Level ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Negative Group ◽  
One Way Anova

ABSTRACTChronic diabetes mellitus is a disease of increased blood glucose levels in a long time so that it can affect sexual function such as damage spermatogenesis and aphrodisiac function. Parijoto has flavonoid content. This study aimed to determine the effect of ethanolic extract of parijoto (Medinilla speciosa Blume) fruit in blood glucose level and the sexual function in alloxan-induced diabetic male rats. The design of the study was post test only control group design, 25 male wistar rats aged 2 months with BB 100-200 gram divided into 5 groups. Group 1 (normal control), group 2 (negative control) induced alloxan 100mg / kgBB, groups 3, 4, and 5 were alloxan induced and administered EEBP at doses of 100 mg / kgBW, 250 mg / kgBW, and 500 mg / kgBW. Alloxan-induced rats were left for 4 weeks to achieve chronic DM. Data analysis of blood glucose level and spermatozoa quality using one way anova followed by LSD post hoc. While aphrodisiac of introducing and climbing using one way anova continued by LSD and coitus using kruskal wallis. The results showed that there were significant differences between negative control with normal group, III, IV, and V. The effect of ethanolic extract of parijoto fruit on blood glucose level and sexual function in alloxan-induced diabetic male rats showed no difference between treatment group of 100 mg / kgBW with negative group, and there were significant differences between treatment group of 250 mg / kgBW and 500 mg / kgBW with negative group. Ethanolic extract of parijoto fruit at dose 250 mg / kgBB and 500 mg / kgBB decrease blood glucose level, improve and increase sexual function in alloxan-induced diabetic male rats.

Pengujian Efektivitas Penyembuhan Luka Mencit Diabetes Melitus Yang Diberikan Sediaan Krim Ekstrak Etanol Daun Bandotan

2016 ◽  
Vol 6 (1) ◽  
pp. 50
Author(s):  
Ria Afrianti ◽  
Dedi Nofiandi ◽  
Dira Dira ◽  
Widya Ulfa
Keyword(s):  
Wound Healing ◽  
Ethanolic Extract ◽  
Positive Control ◽  
Negative Control ◽  
Collagen Fibers ◽  
Group Iv ◽  
Ageratum Conyzoides ◽  
Group V ◽  
Group Iii ◽  
Group I

Bandotan plants (Ageratum conyzoides (L.) L.) commonly used as agent in the treatment of inflammatory, wound, and also used used as analgesic, stimulant and tonic. In this study we carried out a test of the effectiveness of the cream (w/o) of ethanolic extract of bandotan leaves in wound healing in streptozocin-induced hyperglycemia mice. Animals were divided into five groups: Group I (without induced, given cream base w/o), group II (induced, given cream base w/o), group III (induced, given cream containing 3% extract), group IV (induced, given cream containing 5% extract) and group V (induced, given cream containing 7% extract). Animals also divided into three subgroups based on time of decapitation on 7th, 14th and 21th days. The parameters observed were the percentage of wound healing and collagen fibers density scores. Results were analyzed by Two-Way ANOVA. The results showed that the cream in concentration of 3%, 5% and 7% could affect wound healing in hyperglycemia mice. The percentage of wound healing of the cream in concentration of 3%, 5% and 7% were significantly different (p<0,05) with positive control, and density scores of collagen fibers was also significantly different (p<0,05) from the positive control and negative control. However, the best results was obtained from cream containing 5% extract which perrcentage of wound healing reached 98.5% on 21th day and got a score of 3 for density of collagen fibers that had been formed on the 14th day. 

scholarly journals EFEK ROSELLA MERAH KERING TERHADAP KADAR MDA SERUM TIKUS STRAIN WISTAR YANG DI PAPAR ASAP ROKOK

2018 ◽  
Vol 1 (2) ◽  
Author(s):  
Novera Herdiani ◽  
Wiwik Afridah
Keyword(s):  
Free Radicals ◽  
Cigarette Smoke ◽  
Wistar Rats ◽  
Day Treatment ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Control Treatment ◽  
Serum Test ◽  
One Way Anova

Abstract: Cigarette smoke contains free radicals. Currently the number of smokers in Indonesia arestill high. This study aims to analyze the effects of red rosella of dried on the prevention of theincrease of Malondialdehyde (MDA) serum of strain wistar rats exposed to cigarette smoke. Thetotal sample of 24 male rats and divided into four groups: negative control, positive control, treatment of red rosella on dried dose 540 mg/bw, and red rosella on dried dose 810 mg/bw. Negativecontrol only given with standard feed. Positive control given by standard feed and exposed to 2cigarettes a day. Treatment group were feed by standard and red rosella of dried in the morning andexposed to 2 cigarettes after that. This study was conducted for 21 days. At the end of the study,blood serum was analyzed to determine MDA. The results of the study of MDA serum test isanalyzed with One Way ANOVA and followed by Tukey LSD test at 5% level. The giving of redrosella on dried dose 540 mg/bw and red rosella on dried dose 810 mg/bw can significantly preventthe increase of MDA serum in strain wistar rats exposed to cigarette smoke.

A Study on the Effect of Samoa Extract (Cleome droserifolia) on Sperm Quality in Male Albino Rats Exposed to Pyrethroid

2021 ◽  
pp. 39-45
Author(s):  
Nura I. Al-Zail ◽  
Salah F. Kamies
Keyword(s):  
Sperm Quality ◽  
Group Iv ◽  
Male Rats ◽  
Control Group ◽  
Albino Rats ◽  
Protective Agent ◽  
Group V ◽  
Group Iii ◽  
Group I ◽  
Induced Changes

Pyrethroid cyhalothrin (PC) is an insecticide that is used worldwide for pest control in agriculture and household use. Samoa extract (SE) is a potent antioxidant protecting cells from oxidative stress. The present study investigates the protective and therapeutic effect of SE on PC-induced changes in sperm quality in male rats. Fifty adult male albino rats were divided into five groups: group I: served as control; group II: received PC i.p. only (6.2 mg/kg b.wt.); group III: received SE only (100 mg/kg b.wt., p.o.) for eight weeks; group IV: received SE as a protective agent daily for eight weeks, then followed by the administration of PC (i.p.) three times a week for two weeks; group V: exposed to PC (i.p.) three times a week for two weeks, then treated with the SE daily for 8 weeks. Results showed that PC caused markedly impaired sperm quality (a count, viability, motility, and abnormality). Compared to PC-treated animals, SE in the protective group markedly restored the alteration of sperm indices. However, SE in the curative group was found to be less effective in restoring PC-induced alterations. In conclusion, the data of this study revealed that the SE as a protective agent is more effective than as a therapeutic agent. Keywords: Samoa; Pyrethroid; Sperm quality; Rat

scholarly journals Effect of concurrent exposure of toxic concentrations of lead and endosulfan on oxidative stress indices in rats

2021 ◽  
Vol 10 (3) ◽  
pp. 192-195
Author(s):  
Ranganathan V ◽  
◽  
Malik JK ◽  
Rao GS ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reduced Glutathione ◽  
Group Iv ◽  
Male Rats ◽  
Technical Grade ◽  
Stress Indices ◽  
Group Iii ◽  
Group I ◽  
Male Wistar Rats ◽  
Toxic Concentrations

The effect of concurrent exposure of toxic concentrations of lead and endosulfan were evaluated on oxidative stress parameters in male wistar rats. Group I served as untreated control whereas Group II received drinking water containing lead as lead acetate @1000 ppm (Pb1000). Group III was exposed to feed containing technical grade endosulfan @ 100 ppm (E100). Group IV was exposed to Pb (1000) +E (100). All the treatments were given daily for 28 days. Combination of lead and endosulfan modified the indices of oxidative stress in the parameters such as lipid peroxidation, reduced glutathione, superoxide dismutase and catalase in rats as compared to their individual compounds. The results suggest that the combination of these individual compounds may have the potential to modify oxidative stress produced by single compounds in male rats

scholarly journals PENGARUH PEMBERIAN EKSTRAK AKAR PASAK BUMI (EURYCOMA LONGIFORA JACK) TERSTANDAR TERHADAP GAMBARAN HISTOPATOLOGIK TESTIS TIKUS WISTAR

2017 ◽  
Vol 13 (3) ◽  
Author(s):  
Condro Suro Miyarso ◽  
Tri Cahyani Widiastuti ◽  
Naelaz Zukhruf Wakhidatul Kiromah
Keyword(s):  
Body Weight ◽  
Testosterone Level ◽  
Positive Control ◽  
Negative Control ◽  
Male Rats ◽  
Histopathological Changes ◽  
Group Iii ◽  
Eurycoma Longifolia ◽  
Group I ◽  
Eurycoma Longifolia Jack

The root of pasak bumi (Eurycoma longifolia, Jack) is one of plant from Indonesia known as aphrodisiac. The study about its standardized extract as aphrodisiac was show increasing libido, testosterone level, FSH and LH on male rats. This study was conducted to evaluate testosterone level and histopathological changes of the testes of male rats of standardized extract of pasak bumi root. There are 50 old male rats which ages 3-4 months old divided to five groups. Group I as negative control was administered aquadestilata. Group II as positive control was administered testosterone (Andriol®). Group III, IV and V ware given standardized extract of pasak bumi root at the dose 50, 100 and 200 mg/kg body weight Respectively. The extract was given orally twice a day for six days and forty nine days and then the testes was taken out on 7th and 50th day. The histopathological of the testes was evaluated using quantitative parameter by the presence of testes weight, number and diameter of Leydig cells. Testosterone level was taken on 7th and 50th day used ELISA method. The results were analyzed using parametric test, one way ANOVA (P<0,05), post hoc Dunnet (2-sided) (P < 0,05). The result of this study shows that the standardized extract of pasak bumi root was not effect on histopathological changes of the testes but it could increased the level of testosterone m at the dose 100 and 200 mg/kg body weight.  Key word : Eurycoma longifolia, Jack, aphrodisiac, histopathological testes testosteron

scholarly journals Comparative Evaluation of Apical Sealing Ability of Different Obturation Techniques by Confocal Microscopy: An In Vitro Study

2020 ◽  
Vol 8 (02) ◽  
pp. 55-59
Author(s):  
Reetu Arora ◽  
Yogesh Kumar ◽  
Neetu Jindal ◽  
Renu Aggarwal ◽  
Kavneet Takhar
Keyword(s):  
Root Canal ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Group Iv ◽  
Control Group ◽  
Group Vi ◽  
Group V ◽  
Group Iii ◽  
Group I

Abstract Introduction The aim of obturation in the root canal is to completely seal the canal space to eliminate all the portals of entry and exit between root canal and periodontal space. Various techniques have been developed to achieve a hermetic seal. Materials and Methods As many as 150 extracted human maxillary central incisors were taken for the study. Biomechanical preparation was done up to F5 protaper file. According to different obturation techniques, samples were divided into six groups, keeping 30 samples in experimental and 15 samples in control groups. Group I–Lateral Condensation, Group II–Thermafil, Group III–Beefill, Group IV–GuttaFlow, Group V–Positive Control group, Group VI–Negative Control group. After obturation, the samples were immersed in 2% Rhodamine-B dye for 24 hours. Each sample was longitudinally sectioned to examine under confocal laser scanning microscope. Statistical Analysis The results were evaluated with ANOVA and posthoc Tukey honest significant difference (HSD) comparison test. Results The mean values of dye penetration of different groups were Group I (Lateral Condensation) 1.51 ± 0.451, Group II (Thermafil) 0.918 ± 0.399, Group III (Beefill) 1.30 ± 0.559. Group IV (GuttaFlow) 0.655 ± 0.396, Group V (Positive Control group) 1.96 ±0.046, Group VI (Negative Control group) 0 ± 0. The lowest mean value of apical microleakage was found in GuttaFlow amongst all experimental groups. Conclusion It can be concluded that the GuttaFlow obturating material exhibited better apical sealing ability with canal walls.

scholarly journals Assessment the Genotoxic Potential of Fluoxetine and Amitriptyline at Maximum Therapeutic Doses for Four-Week Treatment in Experimental Male Rats

2021 ◽  
Vol 30 (1) ◽  
pp. 81-90
Author(s):  
Imad A. Al-Obaidi ◽  
Nada N. Al-Shawi
Keyword(s):  
Bone Marrow ◽  
Dna Damage ◽  
Adult Male ◽  
Negative Control Group ◽  
Negative Control ◽  
Male Rats ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Hydrochloride Solution

Abstract At any moment, the continuous usage of medications can accompanied by DNA damage and the accumulation of such damages can cause serious consequences. Antidepressants are long-term used drugs and the incidence of their genotoxic impacts cannot be excluded. Therefore, this work was designed to investigate the possible genotoxic effects of the commonly used antidepressants (fluoxetine and amitriptyline) in adult male rats. Detection of DNA damage in individual cells was assessed by comet and micronucleus assays in three different cell populations i.e. liver, testis and bone marrow tissues of 24 swiss albino adult male rats. The animals were randomly allocated into three groups of 8 rats each: Group I - rats orally-administered distilled water via gavage tube for four weeks as a negative control. Group II - rats orally-treated with fluoxetine hydrochloride solution (7.2mg/kg/day) via gavage tube for four weeks. Group III - rats orally-treated with amitriptyline hydrochloride solution (27mg/kg/day) via gavage tube for four weeks. The results showed that both drugs (Group II and Group III) induced the same extent of DNA damage, as evidenced by a significantly higher DNA fragmentation in liver and testis tissues with increased frequencies of micronuclei formation in bone marrow tissues as compared with the negative control (Group I). These findings indicates that both Fluoxetine and Amitriptyline have genotoxic potentials and can induce the same extent of cytogenetic damage in rats. Special precautions and medical supervision should be taken in consideration with their uses.

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