scholarly journals Phytochemical Screening and Antidiabetic Activity of N-Hexane, Ethyl Acetate and Water Extract from Durian Leaves (Durio Zibethinus L.)

2019 ◽  
Vol 35 (1) ◽  
pp. 487-490
Author(s):  
Dyna Grace Romatua Aruan ◽  
Tonel Barus ◽  
Ginda Haro ◽  
Rikson Siburian ◽  
Partomuan Simanjuntak
Keyword(s):  
Ethyl Acetate ◽  
Water Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Durio Zibethinus

scholarly journals Identifikasi Senyawa Metabolit Sekunder pada Durian (Durio zibethinus Murr)

2019 ◽  
Vol 1 (1) ◽  
pp. 1-6
Author(s):  
Aji Suteja ◽  
Emmy Harso Kardhinata ◽  
Rosliana Lubis
Keyword(s):  
Thin Layer ◽  
Ethyl Acetate ◽  
Experimental Method ◽  
Thin Layer Chromatography ◽  
Secondary Metabolite ◽  
Phytochemical Screening ◽  
Screening Analysis ◽  
Durio Zibethinus ◽  
Rf Values ◽  
Layer Chromatography

This study aims to determine how to identify the types of secondary metabolite compounds contained in durian leaves. The study was conducted by an experimental method by identifying secondary metabolite compounds on durian leaves using phytochemical screening. The sample criteria used were the leaf buds and all the leaf strands (except the leaf bones). The results of the study showed that of the three types of durian leaves namely copper, baskets and Sp A. There are several types of secondary metabolite compounds including alkaloids, steroids, and terpenoids. Phytochemical screening analysis was carried out using thin layer chromatography by showing RF values on three types of durian leaves using methanol and ethyl acetate solvents in a ratio of 3: 1. RF value on copper durian is 0.97, bakul durian is 0.95 and Sp durian. A 0.94.

scholarly journals A STUDY ON PHYTOCHEMICAL SCREENING, ANTIOXIDANT, ANTIMICROBIAL AND α-ΑMYLASE INHIBITORY ACTIVITIES OF CRUDE EXTRACTS OF THE STEM BARK OF PISONIA GRANDIS, R.BR

2017 ◽  
Vol 10 (11) ◽  
pp. 129
Author(s):  
Vishu Mohan ◽  
Mohan Kumar Ramasamy ◽  
Srikalyani Vemuri ◽  
Ilango Kaliappan
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Stem Bark ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Phytochemical Screening ◽  
Flavonoid Content ◽  
Phenolic And Flavonoid

  Objective: The aim of the present study is to determine the phytochemical screening, antioxidant activity and α-amylase inhibitory activity of the crude hexane, ethyl acetate and ethanolic stem bark extract of Pisonia grandis.Methods: The evaluation of antioxidant and antimicrobial activity, total phenolic, and flavonoid content were assessed using 2,2-diphenyl-1- picrylhydrazyl, Folin–Ciocalteu’s reagent, and aluminum chloride assay, respectively. The antidiabetic activity was assessed for porcine pancreatic α-amylase for the stem bark of P. grandis. Results: Phytochemical screening confirmed the presence of phenolic, flavonoids, tannins, saponins, terpenoids, and steroids in all the three extracts. The antioxidant activity showed 148.2 μg/ml, total phenolic content (gallic acid equivalent), 0.0665±0.0002 mg/g, flavonoid content (quercetin equivalent), 0.6061±0.1817 mg/g, and inhibitory concentration 50% values were found to be 40.42 μg/ml and showed better in ethyl acetate extract. The antidiabetic activity exhibited mimic action with insulin due to the presence of pinnatol in the stem bark and leaves of P. grandis. Conclusion: P. grandis stem bark crude ethyl acetate extract showed strong antioxidant activity, high phenolic, and flavonoid content. The antimicrobial activity was studied in both Gram-positive and Gram-negative strains against ampicillin and rifampicin as reference drugs. Antidiabetic activity shows effective result by α-amylase inhibitory activity.

scholarly journals The Antidiabetic and Anti-microbial Activity Analyses in The Extract of Methanol, Ethyl Acetate, and Hexane from Flesh of Matoa (Pometia pinnata J. R. Forst. & G. Forst)

2021 ◽  
Vol 18 (22) ◽  
pp. 485
Author(s):  
Henny Rochaeni ◽  
Candra Irawan ◽  
Hanafi Hanafi ◽  
Poppy Sri Lestari ◽  
Lilis Sulistiawaty ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Inhibition Zone ◽  
Test Method ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Phytochemical Screening ◽  
Microbial Activities ◽  
Hexane Extracts ◽  
Inhibitory Power ◽  
Ethyl Acetate Extracts

The current research was designed to examine the antidiabetic and anti-microbial activities in the extracts of methanol, ethyl acetate and hexane from Flesh of Matoa (Pometia pinnata J. R. Forst. & G. Forst) to find out the best solvent to produce antidiabetic and anti-microbial active compounds.  In the phytochemical screening, hexane extracted alkaloids and terpenoids, while ethyl acetate extracted saponin and methanol extracted tannin, phenol and terpenoids. In the antidiabetic activity by a-glucosidase inhibition methods, ethyl acetate showed the highest percentage of inhibition and the smallest value of IC50, followed by hexane and methanol compared to the Quercetin as the control group. The maximum percentage inhibition was 36.74 ± 0.07 for 100 µg/mL of concentration. The minimum value of IC50 was observed at 159.74 ± 0.65 µg/ml compared to the control group 6.04 ± 0.14 µg/ml. The smaller the IC50 value, the stronger the antidiabetic activity. Anti-microbial inhibition activities assessed using anti-microbial diffusion test method. The hexane and ethyl acetate extracts provide an inhibition of fungi and bacteria compared to Ketoconazole and Amoxicillin as the standard. The maximum fungi inhibition zone was observed at 4 mm for A. flavus and 9 mm for A. niger by Hexane extracts compared to the standard 14 and 24 mm. The maximum bacteria inhibition zone was observed at 7 mm for B. cereus by both of hexane and ethyl acetate extracts compared to the standard 5 mm. Compared to the anti-microbe inhibitory power categories, hexane and ethyl acetate was on the range of weak and moderate. The methanol extract could not inhibit all types of microbe. This study concluded that various extract of flesh of Matoa showed antidiabetic and anti-microbial activities at different value with varying potencies. The ethyl acetate extract was found to be more potent among all, followed by hexane and methanol. HIGHLIGHTS Phytochemical screening of Flesh of Matoa (Pometia pinnata R. Forst. & G. Forst) produced alkaloids, terpenoids, saponin, tannin, phenol and terpenoids In the antidiabetic activity by a-glucosidase inhibition methods, ethyl acetate showed the highest percentage of inhibition and the smallest value of IC50, followed by hexane and methanol The hexane and ethyl acetate extracts provide an inhibition of fungi and bacteria compared to Ketoconazole and Amoxicillin as the standard GRAPHICAL ABSTRACT

scholarly journals Solvent Extraction and Phytochemical Screening of Seeds, Coats, Pods and Leaves of Moringa Plant

2021 ◽  
Vol 6 (2) ◽  
Keyword(s):  
Ethyl Acetate ◽  
Water Extract ◽  
Mesh Size ◽  
Ethanol Extract ◽  
Volatile Oil ◽  
Chloroform Extract ◽  
Reducing Sugar ◽  
Phytochemical Screening ◽  
Plant Samples ◽  
Bioactive Ingredients

The potency of six different solvents in extracting phytochemicals from the seeds, coats, pods and leaves of moringa plant was investigated. The seeds, coats, pods and leaves of the plant were cut into smaller pieces, air-dried, ground into powdery sample, sieved with 40 mm mesh size and properly labelled. Each sample was individually extracted using six different solvents (methanol, ethanol, chloroform, ethyl acetate, water and acetone) at ratio 1: 10 for 72 h. Each solvent extract was screened for twelve phytochemicals (alkaloid, flavonoid, saponin, cardiac glycoside, reducing sugar, tannin, quinone, volatile oil, phenol, terpenoid, phlobatannin and steroid). It was observed that the seeds and leaves of moringa plant were richest in phytochemicals followed by moringa pods and the least was in moringa coat. In all the six solvents used, thirty-four bioactive ingredients were detected in seeds and leaves of moringa plant while twenty-eight phytochemicals were obtained in moringa pods and twenty-one bioactive ingredients were gotten from moringa coats. In all the plant samples, twenty-three bioactive ingredient were detected in ethanol extract; twenty-one were obtained in each of acetone, ethyl acetate and methanol extracts; water extract had sixteen phytochemicals and chloroform extract had fifteen bioactive ingredients. Among the solvents used for extraction for all the plant samples, ethanol ranked first while acetone, ethyl acetate and methanol ranked second, water ranked third and chloroform was the least in ranking.

scholarly journals Potential of medang reso (Cinnamomum parthenoxylon) as raw material source for antidiabetic drugs

2021 ◽  
Vol 914 (1) ◽  
pp. 012074
Author(s):  
Asmaliyah ◽  
E E W Hadi ◽  
E Novriyanti
Keyword(s):  
Herbal Medicine ◽  
Herbal Medicines ◽  
Water Extract ◽  
Raw Material ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Indigenous Plants ◽  
Material Source

Abstract Diabetics is known as a dangerous comorbid disease in covid 19 patience. Many herbal medicines are used to prevent diabetes. Recently, the use of herbal medicine increased rapidly. In Indonesia, many indigenous plants have been used as source of raw material of herbal medicine including Cinnamomum parthenoxylon. Previous study reported that in vitro administration of C. parthenoxylon extract could reduce the sugar level and increased the plasma insulin level. Data on phytochemistry and bio-activity of this species are unavailable yet. Therefore, this study examined the phytochemical constituents and antidiabetic activity of their leaves. The leaves of C. pathenoxylon were collected from Bangka Belitung and South Sumatra for phytochemical screening and in vitro study. The phytochemical screening showed that constituents of flavonoids, steroids, tannins, alkaloids, and saponins were contained in the water extract of C. pathenoxylon leaves. The study indicated that extract leaves of C. pathenoxylon contains antidiabetic activity. In conclusion, C. pathenoxylon has the potential to be utilized as a source of raw material for diabetes medication. However, further in vivo and human administration studies are essentially required.

scholarly journals PHYTOCHEMICAL SCREENING AND ANTIDIABETIC ACTIVITY OF DIFFERENT LEAF EXTRACTS FROM LOTUS (NELUMBO NUCIFERA GAERTN.) IN STREPTOZOTOCIN INDUCED MICE

2017 ◽  
Vol 10 (12) ◽  
pp. 190
Author(s):  
Ruth Mayana Rumanti ◽  
Marline Nainggolan ◽  
Urip Harahap
Keyword(s):  
Ethyl Acetate ◽  
Leaf Extract ◽  
Ethyl Acetate Extract ◽  
Screening Method ◽  
Nelumbo Nucifera ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Leaf Extracts ◽  
Lotus Leaf ◽  
Leaf N

  Objective: The present study is to evaluate the antidiabetic activity of various extract of Lotus (Nelumbo nucifera Gaertn.) leaf using hexane, ethyl acetate, and ethanol organic solvent.Methods: Standard phytochemical screening method and streptozotocin induced diabetic mice.Result: The phytochemical screening showed only ethyl acetate of lotus leaf extract contains the flavonoids. The ethyl acetate lotus leaf extract (dose level 150 mg/kg) showed a significant reduction of the blood glucose.Conclusion: The ethyl acetate extract of lotus leaf (N. nucifera Gaertn.) had the hypoglycemic effect on diabetic induced mice.

scholarly journals Effect of Extraction Time and Solvent Power on Phytochemical Screening and Antioxidant Activity of Momordica charantia L. Fruit Extracts

2019 ◽  
Vol 31 (3) ◽  
pp. 647-650
Author(s):  
Rayees Ahmad Malik ◽  
Mabroukah Massuod Saad ◽  
Surabhi Tiwari
Keyword(s):  
Nitric Oxide ◽  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Water Extract ◽  
Ethanolic Extract ◽  
Momordica Charantia ◽  
Phytochemical Screening ◽  
Time Duration ◽  
Fruit Extracts ◽  
Medium Time

This work reports the analysis of phytochemical screening and antioxidant activity of Momordica charantia L. fruit extracts at different extraction conditions. The standard procedures are achieved by ferric reducing antioxidant, nitric oxide scavenging assay methods. Extraction under different maceration periods viz. 6, 12, 24 and 48 h organic solvent especially ethanol was found more effective followed by water extract, acetone and ethyl acetate. The maximum number of phytochemicals were observed at 12 h followed by 6 h. Hence, the medium time duration influences the extraction of phytochemicals at 12, 6 h rather than 24 and 48 h. The ferric reducing antioxidant activity was observed maximum at 12 h (0.156 ± 0.012, 79.31 %) followed by 6 h (0.149 ± 0.022, 71.26 %), 24 h (0.143 ± 0.032, 64.36 %) and 48 h (0.139 ± 0.023, 59.77 %) in ethanolic extract. Similarly the nitric oxide scavenging activity was observed maximum at 12 h (0.093 ± 0.023, 76.53 %) followed by 6 h (0.113 ± 0.043, 71.17 %), 24 h (0.134 ± 0.031, 65.81 %) and 48 h (0.161 ± 0.053, 58.92 %) in ethanolic extract followed by aqueous, acetone, ethyl acetate, petroleum ether and hexane extracts at 12 h with respect to standard (ascorbic acid). The results show that the ethanol is more effective for extraction of phytochemicals followed by water extract. The maximum number of phytochemicals are obtained at medium time duration (12 h) in ethanolic extract. Hence demonstrates that fruit extracts are important sources of natural antioxidant agents.

Phytochemical screening for identification of bioactive compounds of Lantana camara Linn. (Verbenaceae) responsible for larvicidal action against Aedes triseriatus Say, 1823 (Diptera : Culicidae)

ENTOMON ◽  
2019 ◽  
Vol 44 (2) ◽  
pp. 117-126
Author(s):  
Rhitayu Chakraborti ◽  
Probir Kumar Bandyopadhyay
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Active Ingredient ◽  
Crude Extract ◽  
Ethyl Acetate Extract ◽  
Lantana Camara ◽  
Mosquito Larvae ◽  
Aedes Triseriatus ◽  
Phytochemical Screening ◽  
Lc50 Value

Study to assess the larvicidal property of Lantana camara leaves against Aedes triseriatus larvae found that the ethyl acetate extract had profound larvicidal action with the crude extract having a LC50 value of 409.831ppm. GC-MS analysis of the ethyl acetate extract confirmed the presence of twenty-one compounds out of which beta-caryophyllene covered the highest percentage of the chromatogram area. Further tests with beta-caryophyllene against the mosquito larvae proved it to be the active ingredient of L. Camara with a LC50 value of 104.243ppm.

scholarly journals A study on Antioxidant Properties of Different Extracts from Kitaibelia balansae

Proceedings ◽  
2019 ◽  
Vol 40 (1) ◽  
pp. 23
Author(s):  
Zengin ◽  
Aygun ◽  
Aktumsek
Keyword(s):  
Ethyl Acetate ◽  
Human Body ◽  
Antioxidant Properties ◽  
Water Extract ◽  
Antioxidant Ability ◽  
Chemical Methods ◽  
Potential Source ◽  
First Time ◽  
Antioxidant Effects ◽  
Ethyl Acetate Extracts

Nowadays, knowledge of ancient botanical medicinal practices and application of modern phytochemical techniques have provided the excellent tools for the purification and structural elucidation of various phyto-compounds, which, in turn, has given insights into their mode of action on the human body. This study has been designed to investigate for the first time the antioxidant effects of the ethyl acetate, methanolic, and water extracts of Kitaibelia balansae. Different chemical methods were performed and the observed abilities depend on the solvent used. The best antioxidant ability was noted in water extract, followed by methanol and ethyl acetate extracts. The highest level of phenolic was also detected in water extract. The present findings suggest that K. balansae can be considered as a potential source of bioactive compounds for novel phytopharmaceuticals development

scholarly journals Identification of Phytochemicals from the Water Extract of Eurycoma longifolia Roots using Solid-Liquid and Liquid-Liquid Extraction Based Fractionation Techniques

2021 ◽  
Vol 68 (4) ◽  
pp. 765-772
Author(s):  
Lee Suan Chua ◽  
Abirame Segaran ◽  
Hoi Jin Wong
Keyword(s):  
Ethyl Acetate ◽  
Solid Phase ◽  
Water Extract ◽  
Reversed Phase ◽  
Liquid Extraction ◽  
Acetone Precipitation ◽  
Eurycoma Longifolia ◽  
Liquid Liquid Extraction ◽  
Saponin Content ◽  
Solid Liquid

Phytochemicals in the water extract of Eurycoma longofolia roots were identified using both solid-liquid and liquid-liquid extraction based fractionation techniques. A reversed phase C18 solid phase extraction (SPE) was used as solid-liquid extraction, whereas solvent partition was applied as liquid-liquid extraction. Total saponin was increased after fractionation. A few known quassinoids; eurycomanone, 13α(21)-epoxyeurycomanone, pasakbumin D, 13β,18-dihydroeurycomanol and 13β,21-dihydroxyeurycomanol were identified from the 40% and 60% methanol fractions of SPE. Solvent partition extract using ethyl acetate was found to have the highest saponin content compared to butanol and chloroform fractions. Subsequent acetone precipitation of the organic fractions recovered a formylated hexose trimer and other saccharide-containing compounds. Ethyl acetate effectively recovered saponins from E. longofolia water extract using liquid-liquid extraction followed by acetone precipitation.

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