Characterisation and chemometric evaluation of 17 elements in ten seaweed species from Greenland

Several Greenland seaweed species have potential as foods or food ingredients, both for local consumption and export. However, knowledge regarding their content of beneficial and deleterious elements on a species specific and geographical basis is lacking. This study investigated the content of 17 elements (As, Ca, Cd, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Ni, P, Pb, Se and Zn) in 77 samples of ten species (Agarum clathratum, Alaria esculenta, Ascophyllum nodosum, Fucus distichus, Fucus vesiculosus, Hedophyllum nigripes, Laminaria solidungula, Palmaria palmata, Saccharina latissima and Saccharina longicruris). Element profiles differed between species but showed similar patterns within the same family. For five species, different thallus parts were investigated separately, and showed different element profiles. A geographic origin comparison of Fucus species indicated regional differences. The seaweeds investigated were especially good sources of macrominerals (K > Na > Ca > Mg) and trace minerals, such as Fe. Iodine contents were high, especially in macroalgae of the family Laminariaceae. None of the samples exceeded the EU maximum levels for Cd, Hg or Pb, but some exceeded the stricter French regulations, especially for Cd and I. In conclusion, these ten species are promising food items.

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Characterisation and chemometric evaluation of 17 elements in ten seaweed species from Greenland

10.1101/2020.11.27.400861 ◽

2020 ◽

Author(s):

Katharina Johanna Kreissig ◽

Lisbeth Truelstrup Hansen ◽

Pernille Erland Jensen ◽

Susse Wegeberg ◽

Ole Geertz-Hansen ◽

...

Keyword(s):

Geographic Origin ◽

Saccharina Latissima ◽

Palmaria Palmata ◽

Food Ingredients ◽

Seaweed Species ◽

Fucus Distichus ◽

Alaria Esculenta ◽

The Family ◽

Species Specific ◽

The Eu

AbstractSeveral Greenland seaweed species have potential as foods or food ingredients, both for local consumption and export. However, knowledge regarding their content of beneficial and deleterious elements on a species specific and geographical basis is lacking. This study investigated the content of 17 elements (As, Ca, Cd, Cr, Cu, Fe, Hg, I, K, Mg, Mn, Na, Ni, P, Pb, Se and Zn) in 77 samples of ten species (Agarum clathratum, Alaria esculenta, Ascophyllum nodosum, Fucus distichus, Fucus vesiculosus, Hedophyllum nigripes, Laminaria solidungula, Palmaria palmata, Saccharina latissima and Saccharina longicruris). Element profiles differed between species but showed similar patterns within the same family. For five species, different thallus parts were investigated separately, and showed different element profiles. A geographic origin comparison of Fucus species indicated regional differences. The seaweeds investigated were especially good sources of macrominerals (K > Na > Ca > Mg) and trace minerals, such as Fe. Iodine contents were high, especially in macroalgae of the family Laminariaceae. None of the samples exceeded the EU maximum levels for Cd, Hg or Pb, but some exceeded the stricter French regulations, especially for Cd and I. In conclusion, these ten species are promising food items.

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A Warmer Arctic Compromises Winter Survival of Habitat-Forming Seaweeds

Frontiers in Marine Science ◽

10.3389/fmars.2021.750209 ◽

2022 ◽

Vol 8 ◽

Author(s):

Francisco J. L. Gordillo ◽

Raquel Carmona ◽

Carlos Jiménez

Keyword(s):

Dark Period ◽

Early Spring ◽

The Arctic ◽

Saccharina Latissima ◽

Photosynthetic Parameters ◽

Total N ◽

Alaria Esculenta ◽

Biomass Loss ◽

Photosynthetic Potential ◽

Species Specific

Continuous winter darkness at a latitude of 79°N was simulated in cultures of four species of Arctic seaweeds at 3 and 8°C. The laminarians Saccharina latissima and Alaria esculenta, and the rhodophytes Phycodrys rubens and Ptilota gunneri were monitored for 4 months in total darkness and after 1 week following light return in early spring, under controlled laboratory conditions. Biomass loss during darkness was enhanced by the high temperature in all species. At 8°C, the two laminarians were unable to resume growth upon re-illumination. Alaria esculenta showed new blade production by the end of the dark period, but only at 3°C. In all species, the photosynthetic ability was sustained, not suspended, during the whole dark period. P. rubens exhibited lower photosynthetic potential at 8°C than at 3°C during the darkness period, but it was able to recover its O2 evolving potential upon re-illumination, as P. gunneri and S. latissima did, but the latter only at 3°C. The reactivation of photosynthesis seemed to involve photosystem II over photosystem I, as 7 d of photoperiod after the prolonged darkness was not enough to fully recover the PAM-related photosynthetic parameters. Only small changes were recorded in the internal chemical composition (total C, total N, carbohydrates, proteins, and lipids), but species-specific differences were observed. Unlike subarctic areas with an operating photoperiod along the year, a warmer polar night might pose a limit to the ability of multi-year seaweeds to occupy the new ice-free illuminated areas of the Arctic coasts, so that newcomers will potentially be restricted to the spring-summer season.

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Identification of fatty acids in fractionated lipid extracts from Palmaria palmata, Alaria esculenta and Saccharina latissima by off-line SPE GC-MS

Journal of Applied Phycology ◽

10.1007/s10811-020-02193-2 ◽

2020 ◽

Vol 32 (6) ◽

pp. 4251-4262

Author(s):

Lena Foseid ◽

Ingeborg Natvik ◽

Hanne Devle ◽

Dag Ekeberg

Keyword(s):

Fatty Acids ◽

Neutral Lipids ◽

Essential Fatty Acids ◽

Saccharina Latissima ◽

Palmaria Palmata ◽

Omega 3 ◽

Alpha Linolenic Acid ◽

Alaria Esculenta ◽

Lipid Fractions ◽

Omega 6

AbstractThe fatty acid profiles of the three lipid fractions, neutral lipids (NL), free fatty acids and polar lipids (PL), from the macroalgae Palmaria palmata (wild), Alaria esculenta (cultivated) and Saccharina latissima (cultivated) were studied in light of dietary important fatty acids. Blade and stipe from the macroalgae were collected at the end of the growth season (May/June) at Frøya (Trøndelag, Norway). A total of 51 fatty acids were identified in the algae, including several fatty acids novel to one or more of the algae. The NL and PL fractions of P. palmata were the most promising from a health perspective, due to a high content of eicosapentaenoic acid (10.1 ± 0.5% and 6.6 ± 0.1%, respectively) and no trans-fatty acids. In addition, these fractions had very low omega-6/omega-3 ratios (< 0.1) and can therefore be beneficial for balancing the omega-6/omega-3 ratio in the diet. The NL fraction of A. esculenta had the highest content of monounsaturated- and polyunsaturated fatty acids (20.9 ± 1.4 and 21.8 ± 1.9% of alga, respectively), as well as the highest content of the two essential fatty acids, linoleic acid (5.3 ± 0.4% of alga) and alpha-linolenic acid (2.4 ± 0.2% of alga). Indices related to risk factors for coronary heart disease were most favourable in the NL fraction of S. latissima and the NL and PL fractions of A. esculenta.

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Variability and Potential of Seaweeds as Ingredients of Ruminant Diets: An In Vitro Study

Animals ◽

10.3390/ani9100851 ◽

2019 ◽

Vol 9 (10) ◽

pp. 851 ◽

Cited By ~ 4

Author(s):

Ana de la Moneda ◽

Maria Dolores Carro ◽

Martin R. Weisbjerg ◽

Michael Y. Roleda ◽

Vibeke Lind ◽

...

Keyword(s):

Control Diet ◽

In Vitro Study ◽

Saccharina Latissima ◽

Palmaria Palmata ◽

Ruminal Fermentation ◽

Laminaria Digitata ◽

Alaria Esculenta ◽

Ruminant Diets ◽

Mastocarpus Stellatus

This study was designed to analyze the chemical composition and in vitro rumen fermentation of eight seaweed species (Brown: Alaria esculenta, Laminaria digitata, Pelvetia canaliculata, Saccharina latissima; Red: Mastocarpus stellatus, Palmaria palmata and Porphyra sp.; Green: Cladophora rupestris) collected in Norway during spring and autumn. Moreover, the in vitro ruminal fermentation of seventeen diets composed of 1:1 oat hay:concentrate, without (control diet) or including seaweeds was studied. The ash and N contents were greater (p < 0.001) in seaweeds collected during spring than in autumn, but autumn-seaweeds had greater total extractable polyphenols. Nitrogen in red and green seaweeds was greater than 2.20 and in brown seaweeds, it was lower than 1.92 g/kg DM. Degradability after 24 h of fermentation was greater in spring seaweeds than in autumn, with Palmaria palmata showing the greatest value and Pelvetia canaliculata the lowest. Seaweeds differed in their fermentation pattern, and autumn Alaria esculenta, Laminaria digitata, Saccharina latissima and Palmaria palmata were similar to high-starch feeds. The inclusion of seaweeds in the concentrate of a diet up to 200 g/kg concentrate produced only subtle effects on in vitro ruminal fermentation.

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Species-diagnostic and species-specific DNA sequences evenly distributed throughout pine and spruce chromosomes

Genome ◽

10.1139/g10-065 ◽

2010 ◽

Vol 53 (10) ◽

pp. 769-777 ◽

Cited By ~ 1

Author(s):

Melanie Mehes-Smith ◽

Paul Michael ◽

Kabwe Nkongolo

Keyword(s):

Dna Sequences ◽

Random Amplified Polymorphic Dna ◽

Inter Simple Sequence Repeat ◽

Issr Markers ◽

Pinus Monticola ◽

The Family ◽

Species Specific ◽

Rapd And Issr ◽

Specific Sequences

Genome organization in the family Pinaceae is complex and largely unknown. The main purpose of the present study was to develop and physically map species-diagnostic and species-specific molecular markers in pine and spruce. Five RAPD (random amplified polymorphic DNA) and one ISSR (inter-simple sequence repeat) species-diagnostic or species-specific markers for Picea mariana , Picea rubens , Pinus strobus , or Pinus monticola were identified, cloned, and sequenced. In situ hybridization of these sequences to spruce and pine chromosomes showed the sequences to be present in high copy number and evenly distributed throughout the genome. The analysis of centromeric and telomeric regions revealed the absence of significant clustering of species-diagnostic and species-specific sequences in all the chromosomes of the four species studied. Both RAPD and ISSR markers showed similar patterns.

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Comparative Cytogenetics in Four Leptodactylus Species (Amphibia, Anura, Leptodactylidae): Evidence of Inner Chromosomal Diversification in Highly Conserved Karyotypes

Cytogenetic and Genome Research ◽

10.1159/000515831 ◽

2021 ◽

pp. 1-11

Author(s):

David S. da Silva ◽

Heriberto F. da Silva Filho ◽

Marcelo B. Cioffi ◽

Edivaldo H.C. de Oliveira ◽

Anderson J.B. Gomes

Keyword(s):

18S Rdna ◽

Repetitive Sequences ◽

Molecular Cytogenetics ◽

Comparative Cytogenetics ◽

The Family ◽

Agnor Staining ◽

Dna Elements ◽

Conventional Cytogenetics ◽

Species Specific ◽

Repetitive Dna Elements

With 82 species currently described, the genus Leptodactylus is the most diverse and representative one in the family Leptodactylidae. Concerning chromosomal organization, this genus represents an interesting and underexplored group since data from molecular cytogenetics are incipient, and little is known about the organization and distribution of repetitive DNA elements in the karyotypes. In this sense, this study aimed at providing a comparative analysis in 4 Leptodactylus species (L. macrosternum, L. pentadactylus, L. fuscus, and Leptodactylus cf. podicipinus), combining conventional cytogenetics (Giemsa staining, C-banding, and AgNOR staining) and mapping of molecular markers (18S rDNA, telomeric and microsatellite probes), to investigate mechanisms underlying their karyotype differentiation process. The results showed that all species had karyotypes with 2n = 22 and FN = 44, except for Leptodactylus cf. podicipinus which presented FN = 36. The 18S rDNA was observed in pair 8 of all analyzed species (corresponding to pair 4 in L. pentadactylus), coinciding with the secondary constrictions and AgNOR staining. FISH with microsatellite DNA probes demonstrated species-specific patterns, as well as an association of these repetitive sequences with constitutive heterochromatin blocks and ribosomal DNA clusters, revealing the dynamics of microsatellites in the genome of the analyzed species. In summary, our data demonstrate an ongoing process of genomic divergence inside species with almost similar karyotype, driven most likely by a series of pericentric inversions, followed by differential accumulation of repetitive sequences.

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Conditioning Family-life at the Intersection of Migration and Welfare: The Implications for ‘Brexit Families’

Journal of Social Policy ◽

10.1017/s004727941700037x ◽

2017 ◽

Vol 46 (4) ◽

pp. 797-814 ◽

Cited By ~ 17

Author(s):

MAJELLA KILKEY

Keyword(s):

Policy Analysis ◽

Family Life ◽

Freedom Of Movement ◽

The Family ◽

Welfare Systems ◽

Gender Divisions ◽

And Gender ◽

The Uk ◽

Over Time ◽

The Eu

AbstractEuropean Freedom of Movement (EFM) was central to the referendum on the UK's membership of the EU. Under a ‘hard’ Brexit scenario, it is expected that EFM between the UK and the EU will cease, raising uncertainties about the rights of existing EU citizens in the UK and those of any future EU migrants. This article is concerned with the prospects for family rights linked to EFM which, I argue, impinge on a range of families – so-called ‘Brexit families’ (Kofman, 2017) – beyond those who are EU-national families living in the UK. The article draws on policy analysis of developments in the conditionality attached to the family rights of non-EU migrants, EU migrants and UK citizens at the intersection of migration and welfare systems since 2010, to identify the potential trajectory of rights post-Brexit. While the findings highlight stratification in family rights between and within those three groups, the pattern is one in which class and gender divisions are prominent and have become more so over time as a result of the particular types of conditionality introduced. I conclude by arguing that, with the cessation of EFM, those axes will also be central in the re-ordering of the rights of ‘Brexit families’.

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Durable Relationship and Family Members “by Analogy” in the European Union

Review of European Studies ◽

10.5539/res.v10n4p175 ◽

2018 ◽

Vol 10 (4) ◽

pp. 175

Author(s):

Tanel Feldman ◽

Marco Mazzeschi

Keyword(s):

Member State ◽

Family Member ◽

Family Members ◽

The European Union ◽

Member States ◽

Registered Partnership ◽

The Family ◽

Definition Of ◽

Union Citizen ◽

The Eu

Rights of residence derived from a durable relationship with an EU citizen, are left to a relatively wide discretion of the Member States. Pursuant to Article 2.2 (b) Directive 2004/38/EC (“Directive”), “the partner with whom the Union citizen has contracted a registered partnership, on the basis of the legislation of a Member State, if the legislation of the host Member State treats registered partnerships as equivalent to marriage and in accordance with the conditions laid down in the relevant legislation of the host Member State” qualifies as family member. Provided that they have a durable relationship (duly attested) with an EU citizen, pursuant to Article 3.2(b), unregistered partners are as well beneficiaries of the Directive. The durable relationship was expressly excluded from the scope of Article 2(2)(b): “Unlike the amended proposal, it does not cover de facto durable relationships” (EU Commission, Document 52003SC1293). Article 3 (2)(a) covers “other family members” (no restrictions as to the degree of relatedness) if material support is provided by the EU citizen or by his partner or where serious health grounds strictly require the personal care of the family member by the Union citizen. Pursuant to Article 3.2, “other family members” and unregistered partners can attest a durable relationship, must be facilitated entry and residence, in accordance to the host Member State’s national legislation. In the light of Preamble 6 Directive, the situation of the persons who are not included in the definition of family members, must be considered “in order to maintain the unity of the family in a broader sense”. The questions discussed in this paper are the following: (i) are Member States genuinely considering the concept of durable relationship in view of maintaining the unity of the family in a broader sense? and (ii) how to overcome legal uncertainty and which criteria, both at EU and at international level, can be taken into account in order to assess whether a durable relationship is genuine and should be granted the rights set forth by the Directive?

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Revisiting Orthotospovirus Phylogeny Using Whole Genomic Data and a Hypothesis for their Geographic Origin and Diversification

10.1101/2020.04.07.031120 ◽

2020 ◽

Author(s):

Anamarija Butković ◽

Rubén González ◽

Santiago F. Elena

Keyword(s):

Phylogenetic Relationships ◽

Geographic Origin ◽

Genome Shuffling ◽

Genomic Data ◽

Genomic Sequences ◽

Genome Database ◽

Evolutionary Forces ◽

The Family ◽

Plant Families ◽

Negative Sense

ABSTRACTThe family Tospoviridae, a member of the Bunyavirales order, is constituted of tri-segmented negative-sense single-stranded RNA viruses that infect plants and are also able of replicating in their insect vectors in a persistent manner. The family is composed of a single genus, the Orthotospovirus, whose type species is Tomato spotted wilt virus (TSWV). Previous studies assessing the phylogenetic relationships within this genus were based upon partial genomic sequences, thus resulting in unresolved clades and a poor assessment of the roles of recombination and genome shuffling during mixed infections. Complete genomic data for most Orthotospovirus species are now available at NCBI genome database. In this study we have used 62 complete genomes from 20 species. Our study confirms the existence of four phylogroups (A to D), grouped in two major clades (A-B and C-D), within the genus. We have estimated the split between the two major clades ∼3,100 years ago shortly followed by the split between the A and B phylogroups ∼2,860 years ago. The split between the C and D phylogroups happened more recently, ∼1,465 years ago. Segment reassortment has been shown to be important in the generation of novel viruses. Likewise, within-segment recombination events have been involved in the origin of new viral species. Finally, phylogeographic analyses of representative viruses suggests the Australasian ecozone as the possible origin of the genus, followed by complex patterns of migration, with rapid global spread and numerous reintroduction events.IMPORTANCEMembers of the Orthotospovirus genus infect a large number of plant families, including food crops and ornamentals, resulting in multimillionaire economical losses. Despite this importance, phylogenetic relationships within the genus were established years ago based in partial genomic sequences. A peculiarity of orthotospoviruses is their tri-segmented negative sense genomes, which makes segment reassortment and within-segment recombination, two forms of viral sex, potential evolutionary forces. Using full genomes from all described orthotospovirus species, we revisited their phylogeny and confirmed the existence of four major phylogroups with uneven geographic distribution. We have also shown a pervasive role of sex in the origin of new viral species. Finally, using Bayesian phylogeographic methods, we assessed the possible geographic origin and historical dispersal of representative viruses from the different phylogroups.

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Emotion Understanding Correlates With Parental Emotional Expressivity in Chinese Youths With Hearing Loss and Typical Hearing

Frontiers in Psychology ◽

10.3389/fpsyg.2021.662356 ◽

2021 ◽

Vol 12 ◽

Author(s):

Yousong Hu ◽

Shuyang Dong ◽

Fang Guan ◽

Outong Chen ◽

Jun Chen ◽

...

Keyword(s):

Socioeconomic Status ◽

Hearing Loss ◽

Language Comprehension ◽

Emotion Understanding ◽

The Self ◽

Regression Coefficients ◽

Emotional Expressivity ◽

Regression Analyses ◽

The Family ◽

The Eu

This study aimed at examining the differences between Chinese youths with hearing loss (HL) and with typical hearing (TH) in emotion understanding (EU), parental emotional expressivity, and the associations between EU and parental emotional expressivity. The participants were 282 youths with HL (14.58 ± 3.42 years old) and 350 youths with TH (11.69 ± 2.49 years old). EU was measured by four visual-mode tasks, of which two involve language comprehension while the others do not. Parents reported positive and negative emotional expressivity on the Self-Expressiveness in the Family Questionnaire. Covariates were controlled for including socioeconomic status, parent gender, youth gender, age, intelligence, and teacher-reported comprehension difficulties. Results showed that the four EU tasks were more challenging for the youths with HL than for the youths with TH. The interaction effect of the two groups × 4 tasks was not significant, suggesting that the differences between the two groups of youths in EU were generally similar across the four tasks. The parents of the youths with HL did not differ from the parents of the youths with TH in how often they displayed positive and negative emotional expressivity. Multigroup regression analyses revealed that negative emotional expressivity was negatively related to EU in the youths with HL but not in the youths with TH. However, these two regression coefficients were not significantly different. Positive emotional expressivity was not related to EU in either group. In conclusion, this study extends the knowledge about the EU of Chinese youths with HL and emotion-related socialization of the parents of these youths.

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