PO-052 Study  on  the  Expression  of  Inflammatory  Factor,  chemotactic  factor  in  the  Repair  of  Skeletal  Muscle  Contusion  in  Mic

Objective To investigate the regulation of muscle inflammatory factors and chemotactic factors during the repair of skeletal muscle contusion in mice. Methods Forty C57 male mice were needed. Eight for control group (C, n=8) and thirty-tow for muscle contusion group (S, n=32). Subsequently, their gastrocnemius muscles were harvested at 0d, 1d, 3d, 7d, 14d after injury. Hematoxylinand eosin (HE) stain were used to assess the changes of muscle morphology. In addition, the gene expression of inflammatory factors and chemotactic factors was analyzed by real-time polymerase chain reaction. Results 1、Morphology of skeletal muscles showed signs of regeneration at 3d post injury. The maximumamount of regeneration muscle fibers appeared during one week post contusion. Two weeks post-injury morphology of myofibers nearly recovered to normal. 2、After skeletal muscle injury, macrophage markers (CD68, CD163, CD206), a variety of inflammatory factors (IL-1, IL-6, IL-10) were up-regulated. 3、chemotactic factors (CCL2, CCL3, CCL5, CCL8, CXCL9, CXCL10, CXCL12, mRNA) were up-regulated。 Conclusions After skeletal muscle contusion, the expression of a variety of chemotactic factors is up-regulated, which promotes macrophage infiltration and produces a variety of inflammatory factors. They may be involved in the inflammatory response and regeneration process after skeletal muscle contusion.

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OR-009 The expression and roles of lncRNAs in the regeneration of skeletal muscle contusion

Exercise Biochemistry Review ◽

10.14428/ebr.v1i2.8693 ◽

2018 ◽

Vol 1 (2) ◽

Author(s):

Lifang Zheng ◽

Peijie Chen ◽

Weihua Xiao

Keyword(s):

Gene Expression ◽

Skeletal Muscle ◽

Muscle Regeneration ◽

Myoblast Differentiation ◽

Control Group ◽

Myogenic Regulatory Factors ◽

Post Injury ◽

Regulatory Factors ◽

Expression Levels ◽

Skeletal Muscle Contusion

Objective In recent years, Accumulating evidence from myoblast differentiation in vitro, cardiotoxin (CTX)-mediated injury or mdx mice suggested that some lncRNAs such as Malat1, H19, linc-MD1, linc-YY1, Sirt1 AS and lnc-mg may modulate myogenesis and muscle regeneration. However, the change of lncRNAs in skeletal muscle contusion and their possible roles are still unclear. We hypothesize that the lncRNAs may be involved in the repair of skeletal muscle contusion. Methods Forty C57BL/6 male mice were randomly divided into two groups, uninjured control group (group C) and muscle contusion group (group S). The mice of group S suffered from contusion injury. All the mice were killed to harvest gastrocnemius at 3, 6, 12 and 24 days post-injury. The gene expression were detected by PCR technique. Gastrocnemius were stained with H & E to evaluate the general morphology. Data were analyzed by One-way analysis of variance, with statistical significance being set at p ≤ 0.05. Results The expression levels of linc-MD1 and Sirt1 AS were significantly higher than that of the uninjured control group at 3, 6 and 12 days post-injury (p<0.01). And Malat1 was highly expressed in the skeletal muscle of the muscle contusion group at 3 days post-injury and continuously up-regulated at 6 days (p<0.01). Moreover, linc-YY1 and H19 were all elevated significantly at 6 days (all p<0.01), but their gene expression levels did not change significantly at 3, 12 and 24 days post-injury, as compared to the uninjured control group. Furthermore, lnc-mg mRNA level did not change significantly in the whole process of regeneration after muscle contusion except the time point of 12 days post-injury which decreased significantly (p<0.01). The expression of myogenic regulatory factors (MyoD, myogenin, myf5, myf6) were studied, they were all elevated significantly at 3 and 6 days (all p<0.01; except myogenin ), and returned to normal at 24 days post-injury, as compared to the uninjured control group. Meanwhile, Pearson correlations showed that there was an correlation between lincRNAs and myogenic regulatory factors mentioned above. Conclusions The expression of myogenic regulatory factors increased significantly after muscle contusion. Meanwhile, varieties of lncRNAs (Malat1, H19, lnc-mg, linc-MD1, linc-YY1, Sirt1 AS) were also up-regulated. Moreover, there was correlation between lncRNAs and myogenic regulatory factors for skeletal muscle regeneration. These results suggest that lncRNAs may play important roles in the regeneration of skeletal muscle contusion.

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Role of the motor nerve in activity-induced enzymatic adaptation in skeletal muscle

AJP Cell Physiology ◽

10.1152/ajpcell.1982.242.5.c272 ◽

1982 ◽

Vol 242 (5) ◽

pp. C272-C277 ◽

Cited By ~ 83

Author(s):

J. Henriksson ◽

H. Galbo ◽

E. Blomstrand

Keyword(s):

Skeletal Muscle ◽

Gastrocnemius Muscle ◽

Tricarboxylic Acid Cycle ◽

Motor Nerve ◽

Oxidative Capacity ◽

Control Group ◽

Marker Enzyme ◽

Muscle Weight ◽

Enzymatic Adaptation ◽

Gastrocnemius Muscles

The sciatic nerve was cut on one side in 11 male cats, and a piece of the nerve was removed. The cats were then divided at random into two groups, a stimulation group (S) of five cats and a control group (C) of six cats. Bilateral electrical stimulation (2 Hz) of the gastrocnemius muscle (directly or via the motor nerve) was carried out in the S cats 4 h/day, 3 days/wk for 4 wk. The voltage delivered was adjusted in each cat so that both gastrocnemius muscles lifted identical loads the same distance. The activity of the tricarboxylic acid cycle marker enzyme succinate dehydrogenase (SDH) per unit of muscle weight more than doubled in response to stimulation both in the intact and the denervated gastrocnemius muscle. Stimulation did not affect the activity of the glycolytic marker enzyme 6-phosphofructokinase (PFK) or muscle capillarization. Denervation resulted in pronounced (approx 50%) fiber atrophy, which was not prevented by the stimulation. It is concluded that the presence of the motor nerve per se is not necessary for an activity-induced adaptation of the oxidative capacity of skeletal muscle.

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Efficacy of Periodontal Tissue Regeneration Combined with Orthodontic Therapy on Periodontitis and Its Influences on Inflammatory Factors in Patients

Journal of Biomaterials and Tissue Engineering ◽

10.1166/jbt.2020.2266 ◽

2020 ◽

Vol 10 (5) ◽

pp. 737-742

Author(s):

Jianxing Han ◽

Junping Dong ◽

Hua Zhao ◽

Yuan Ma ◽

Shuoran Yang ◽

...

Keyword(s):

Postoperative Complications ◽

Tissue Regeneration ◽

Orthodontic Treatment ◽

Inflammatory Factors ◽

Control Group ◽

Inflammatory Factor ◽

Observation Group ◽

Periodontal Tissue ◽

Clinical Attachment Loss ◽

Periodontal Tissue Regeneration

Objective: To assess the effect of periodontal tissue regeneration combined with orthodontic treatment on periodontitis and inflammatory factors. Methods : 100 patients with periodontitis were randomly separated into observation group and control group. Patients were treated with periodontal tissue regeneration in control group and received orthodontic treatment in observation group. The periodontal indexes, X-ray cephalometric indexes, serum inflammatory factor levels, tooth mobility, the postoperative complications, efficacy and life quality were measured. Results: After treatment, levels of clinical attachment loss (CAL), probing depth (PD), sulcus bleeding index (SBI), gingival index (GI), plaque index (PLI), SNB angle, SNA angle, IL-8, IL-5, TNF-α and hs-CRP of patients in observation group were significantly decreased, while ANB angle was significantly elevated (p < 0 05). Meanwhile, the treatment effective rate and quality of life score was significantly improved after treatment in observation group (p < 0 05). Conclusion: Periodontal tissue regeneration combined with orthodontic treatment can significantly improve periodontitis symptoms, promote the recovery of tooth function, reduce inflammation and postoperative complications, and improve the uniformity and appearance of teeth in patients with periodontitis.

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Skeletal muscle stem cell self-renewal and differentiation kinetics revealed by EdU lineage tracing during regeneration

10.1101/627851 ◽

2019 ◽

Author(s):

Bradley Pawlikowski ◽

Nicole Dalla Betta ◽

Tiffany Antwine ◽

Bradley B. Olwin

Keyword(s):

Skeletal Muscle ◽

Stem Cells ◽

Stem Cell ◽

Young Adult ◽

Muscle Injury ◽

Lineage Tracing ◽

Stem Cell Population ◽

Post Injury ◽

Self Renewal ◽

Muscle Stem Cell

SummarySkeletal muscle maintenance and repair is dependent on the resident adult muscle stem cell (MuSC). During injury, and in diseased muscle, stem cells are engaged to replace or repair damaged muscle, which requires the stem cells to exit quiescence and expand, followed by differentiation to regenerate myofibers and self-renewal to replenish the stem cell population. Following an injury, little is known regarding the timing of MuSC (skeletal muscle stem cell) self-renewal, myoblast expansion or myoblast differentiation. To determine the timing and kinetics of these cell fate decisions, we employed DNA-based lineage tracing to label newly replicated cells and followed cell fates during skeletal muscle regeneration. MuSCs activate and expand as myoblasts rapidly following injury, where the majority differentiate into myonuclei, establishing the centrally located myonuclear pool. Re-establishing the majority MuSC pool by self-renewal occurs after 5 days post-muscle injury, accompanied by low levels of myonuclear accretion that generate only peripheral myonuclei. In aged mice, possessing ∼1/2 the number of MuSCs present in young adult mice, the timing of post injury MuSC self-renewal is delayed, and although MuSCs expansion as myoblasts in aged muscle is impaired, the number of MuSC unexpectedly recovers to young adult levels during regeneration. Following an induced muscle injury, we found that myonuclei are generated within the first four days post injury derived from myoblasts expanding from activated MuSCs. Only later during regeneration, from 5 d to 14 d post injury, is the MuSC pool replenished by self-renewal, accompanied by generation of peripheral myonuclei.

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The impact of skeletal muscle injury on the expression of Laminin and its role in regeneration

Baghdad Journal of Biochemistry and Applied Biological Sciences ◽

10.47419/bjbabs.v1i01.29 ◽

2020 ◽

Vol 1 (01) ◽

pp. 26-38

Author(s):

Taghreed Abdul Rasool Ali ◽

Laith Hekmat Zaki ◽

May Fadhil Al- Habib

Keyword(s):

Skeletal Muscle ◽

Muscle Injury ◽

Morphological Changes ◽

P Value ◽

Post Injury ◽

Skeletal Muscle Injury ◽

Dynamic Type ◽

Injury Group ◽

Group B ◽

The Impact

Background:Laminins are high-molecular-weight proteins in the extracellular matrix; it is a major component of the basal lamina, influencing cell differentiation, migration, and adhesion. Laminin affects cell growth, besides effects in wound healing and embryonic development.Objective:The present study aims to assess the histological changes taking place during skeletal muscle healing.Methods:The Extensor digitorum longus muscle of 45 male rabbits was set as a skeletal muscle injury model and examined 3&6 weeks after initiation of injury. These animals were divided into three groups control (A) group with no injury, group (B) at 3rdpost-injury week, group (C) at 6th post-injury week. The muscle tissues were prepared and examined histologically using H&E and immunohistochemically using Laminin antibodies. Aperio image scope software is used to analyze immunohistochemical reactivity quantitatively.The degeneration and regeneration process were overlapping with each other both in time and cellular morphological changes. Early myoblast like cell appearance and new myotube formation was recorded during the 3rd week. By the end of the6th-week postoperatively, the muscle histological maturation and muscle fascicles were noticed.Results:Immunohistochemical reactivity of Laminin antibody showed an intense reactivity in the 3rd-week group while a less intense reactivity in the control and 6th-week groups’.A quantitative assessment of Laminin using Aperio soft wear showed that the 3rd-week group has an intensity of 0.724 ± 0.03 pixel, while the 6th week’s group was 0. 321 ± 0.02 pixel and the control groups was 0.293 ± 0.02 pixel. The differences were statistically significant, P-value 0.0001.Conclusion: The process of regeneration is a dynamic type where degeneration andregeneration superimposed each other.

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Postoperative Effects of Dexmedetomidine on Serum Inflammatory Factors and Cognitive Malfunctioning in Patients with General Anesthesia

Journal of Healthcare Engineering ◽

10.1155/2021/7161901 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Zitan Zhang ◽

Wei Li ◽

Huiqun Jia

Keyword(s):

Elderly Patients ◽

General Anesthesia ◽

Postoperative Cognitive Dysfunction ◽

Inflammatory Factors ◽

Control Group ◽

Inflammatory Factor ◽

Tnf Α ◽

Random Number Table ◽

Factor Α ◽

Necrosis Factor

Objective. To investigate the effects of dexmedetomidine intervention on serum inflammatory factor concentration and postoperative cognitive malfunction in elderly patients with general anesthesia. Methodology. 174 patients with general anesthesia were selected, who were categorized into a control group (HC) and a dexmedetomidine group (HS) using the random number table method, with 87 patients in individual groups. The dexmedetomidine group was pumped intravenously with dexmedetomidine at a loading dose of 1 μg/kg before induction of anesthesia for 15 min, followed by continuous intravenous pumping at a rate of 0.4 μg/kg/h, and the dosing was stopped at 30 min before concluding the surgery. The control group was administered the identical dose of saline in the same manner. Interleukin 6 (IL-6) and tumor necrosis factor α (TNF-α) levels and MMES scores were tested at 1 h before and 24 h after anesthesia. Results. Comparing to HC group, patients in the HS group had lower TNF-α and IL-6 levels at both scheduled points ( P < 0.05). Conclusion. Dexmedetomidine reduced the expression of inflammatory factors in elderly patients with general anesthesia and effectively reduced the incidence of postoperative cognitive dysfunction after general anesthesia surgery.

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PL-003 BMSCs transplantation aggravate inflammation, oxidative stress, fibrosis and impair skeletal muscle regeneration

Exercise Biochemistry Review ◽

10.14428/ebr.v1i1.8073 ◽

2018 ◽

Vol 1 (1) ◽

Author(s):

Xiaoguang Liu ◽

Weihua Xiao ◽

Lifang Zhen ◽

Yongzhan Zhou ◽

Jian Shou

Keyword(s):

Oxidative Stress ◽

Skeletal Muscle ◽

Matrix Metalloproteinases ◽

Inflammatory Cytokines ◽

Muscle Regeneration ◽

Muscle Injury ◽

Treated Group ◽

Skeletal Muscle Regeneration ◽

Skeletal Muscle Contusion ◽

And Oxidative Stress

Objective Skeletal muscle contusion is one of the most common muscle injury in sports medicine and traumatology. Bone marrow mesenchymal stem cells (BMSCs) transplantation is a promising strategy for muscle regeneration. However, the roles of BMSCs, especially the mechanisms involved, in the regeneration of contused skeletal muscle are still not fully recognized. The aim of the study is to evaluate the potential of BMSCs transplantation for muscle regeneration and mechanisms involved after contusion. Methods Ninety-nine C57BL/6J mice were divided into three groups: control group (n=11), muscle contusion and BMSCs treated group (n=44), muscle contusion and sham treated group (n=44). BMSCs were immediately transplanted into gastrocnemius muscles (GMs) following direct contusion. At different time points (3, 6, 12 and 24 days) post-injury, the animals were killed and then GMs were harvested. Morphological and gene expression analyses were used to elevate the effect of BMSCs transplantation and mechanisms involved. Results The results indicate that BMSCs transplantation impairs muscle regeneration, as well as more fibrotic scar formation after skeletal muscle contusion. Furthermore, macrophages, inflammatory cytokines, chemokines, matrix metalloproteinases and oxidative stress related enzymes were significantly increased after BMSCs transplantation. These results suggest that BMSCs transplantation impairs skeletal muscle regeneration and that macrophages, inflammatory cytokines, chemokines, matrix metalloproteinases and oxidative stress related enzymes may be involved in the process. Conclusions BMSCs transplantation aggravates inflammation, oxidative stress and fibrosis, and impairs skeletal muscle regeneration, which shed new light on the role of BMSCs in regenerative medicine and cautions the application of BMSCs for muscle injury.

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The role of inflammatory factors in skeletal muscle injury

Biotarget ◽

10.21037/biotarget.2018.04.01 ◽

2018 ◽

Vol 2 ◽

pp. 7-7 ◽

Cited By ~ 5

Author(s):

Wenjing Ma ◽

Tongtong Xu ◽

Ye Wang ◽

Changyue Wu ◽

Lingbin Wang ◽

...

Keyword(s):

Skeletal Muscle ◽

Muscle Injury ◽

Inflammatory Factors ◽

Skeletal Muscle Injury

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The Correlation Analysis Between the Blood Lipids, Glycosylated Protein Level and Inflammatory Factors of Pregnant Women with GDM During Pregnancy and the Weight of Newborn

10.21203/rs.3.rs-896430/v1 ◽

2021 ◽

Author(s):

Changhua Hu ◽

Lirong Wu ◽

Juan Fang

Keyword(s):

Blood Glucose ◽

Pregnant Women ◽

Research Group ◽

Blood Lipids ◽

Fasting Blood Glucose ◽

Blood Lipid ◽

Adverse Outcomes ◽

Inflammatory Factors ◽

Control Group ◽

Inflammatory Factor

Abstract The study was carried to explore the correlation between blood lipids, blood glucose levels, inflammatory factor and weight of newborn, to provide reference for control of blood glucose in pregnant women with gestational diabetes mellitus (GDM) and early screening of macrosomia. Fifty pregnant women (give birth to newborn) with GDM were selected as research group, and 55 normal pregnant women (give birth to newborn) as control group. Blood lipid levels of TC, TG, HDL-C, LDL-C, the fasting blood glucose (FPG), HbAlc, glycosylated albumin (GA), and expression of inflammatory factor TLR4 of pregnant women in the two groups were monitored. The levels of TG, FPG, HbA1c and GA in pregnant women, the levels of TLR4 in cord blood of newborn, the relative expression of TLR4 protein and TLR4mRNA in the placenta were higher than in control group, and level of HDL-C was lower than the control group. The levels of TC and LDL-C of pregnant women were higher than in control group. Weight of newborn was positively correlated with these all except HDL-C levels (negatively correlated) and no correlation was found with TC and LDL-C. The weight of newborn and incidence of macrosomia in research group were higher compared to control group, and scores of newborns at 1 min and 5 min were lower compared to control group. The results revealed that strengthening the detection of blood lipid and blood glucose during pregnancy can prevent adverse outcomes such as giant babies and improve the quality of birth.

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Protection of Ang1-7 througth MKK/P38MAPKs inflammatory signal pathway on TNF-α stimulated mouse HL-1 cells

10.21203/rs.3.rs-1064422/v1 ◽

2021 ◽

Author(s):

Xuewen Wang ◽

Yuan Cao ◽

Pradeep depark ◽

Deepark Sharma ◽

Guangping Li ◽

...

Keyword(s):

Protein Expression ◽

Signal Pathway ◽

Inflammatory Factors ◽

Control Group ◽

Inflammatory Factor ◽

Tnf Α ◽

Significant Difference ◽

Atrial Myocyte ◽

Group B ◽

Group Control Group

Abstract Background to explore the effect of Ang1-7 througth MKK/P38MAPKs inflammatory signaling pathway on TNF-α-stimulated mouse HL-1 cells. Methods Using TNF-α (100 µg/ml) to establish an inflammatory atrial fibrillation model in HL-1 cell, which derived from mouse atrial myocyte. treated HL-1 cells with different concentrations of Ang 1-7 (0.1, 1 and 10 mmol/L) and divided into 5 groups, namely A group(control group), B group(TNF ), C group(TNF + Ang 1-7 0.1 mmol/L), D group(TNF + Ang 1-7 1 mmol/L ) and E group(TNF + Ang 1-7 10 mmol/L ). Firstly, different concentrations of Ang 1-7 (0.1 mmol/L, 1 mmol/L and 10 mmol/L) were used to stimulate for half an hour, and then TNF-α (100 µg/ml) was added to stimulate for four hours. Both the cells and supernatant were collected. Cells were collected for Western Blotting to detect the protein expression of MKK3, MKK4, MKK6, PMMK4 and PP38. The supernatant was subjected to flow cytometry for detecting multi-inflammatory factors. Results Compared with the A group, the protein expression of MKK3, MKK4, MKK6, PMMK4 and PP38 was statistically significant increased after stimulation with inflammatory factors (TNF-α) (P < 0.05). After intervention with Ang 1-7, the protein expression of MKK3, MKK4, MKK6, PMMK4 and PP38 was statistically significant lower than that of B group (P < 0.05). There is no significant difference of the protein expression of P38 after stimulation with inflammatory factor (TNF-α). Compared with the A group, there was no significant difference in the protein expression of MAS after the stimulation of inflammatory factor (TNF-α). After the intervention of Ang 1-7, the protein expression of MAS was higher than that of the A group and B group, but there was no significant difference (P > 0.05). The expression of MAS protein had an increasing trend, but there was no significant difference (P > 0.05). TGF-β, TNF-α was significantly increased after stimulating factor (TNF-α) was given, but was decreased after the intervention of Ang 1-7, both there were statistically significant (P < 0.05). IL-6 also had the same trend, but there was no significant difference. Conclusion Ang1-7 througth MKK/P38MAPKs inflammatory signal pathway protected on TNF-α stimulated mouse HL-1 cells

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