Bioactive Hybrid Composite Membrane with Enhanced Antimicrobial Properties for Biomedical Applications

2016 ◽  
Vol 66 (4) ◽  
pp. 434 ◽  
Author(s):  
Abin Davis ◽  
Balasubramanian K.
Keyword(s):  
Antimicrobial Properties ◽  
Cost Effective ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Force Microscopy ◽  
Chitosan Matrix ◽  
Effective Manner ◽  
Potential Applications ◽  
Membrane Roughness

<p class="p1"> </p><p class="p2"><span class="s1"> </span><em>Azadirachta indica </em>extract has been explored as an antibiotic in hygienic chitosan matrix system to enhance antimicrobial and medicinal property in a cost effective manner. The hygienic composite system has been successfully fabricated via solvent casting. The antibacterial activities of the hybrid system were examined by agar diffusion method against gram positive <em>S.aureus </em>and gram negative <em>K. pneumoniae. </em>From conventional antibacterial test for 24 h, the system exhibited an excellent antimicrobial activity against both bacterial strains in ranges of 1.2 cm - 1.5 cm for <em>S. aureus </em>and 1.8 cm <em>- </em>2.3 cm for <em>K. pneumoniae </em>Fourier transform infrared spectroscopy revealed successfully embedded A. indica on the chitosan substrate via weak electrostatic interaction, resulting in the easy release of the additive. Moreover, atomic force microscopy showed a membrane roughness of 0.084 nm which confirms the uniform distribution of the additive throughout the membrane. These hybrid membranes have potential applications in skin tissue engineering, wound healing and as coatings for implantable scaffold material.</p>

scholarly journals Chemotherapeutic Potential of Carthamus Oxycantha Root Extract as Antidiarrheal and In Vitro Antibacterial Activities

Antibiotics ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 226 ◽  
Author(s):  
Muhammad Ikram ◽  
Amany Magdy Beshbishy ◽  
Muhammad Kifayatullah ◽  
Adedayo Olukanni ◽  
Muhammad Zahoor ◽  
...  
Keyword(s):  
Magnesium Sulfate ◽  
Castor Oil ◽  
Research Work ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Salmonella Typhi ◽  
Diffusion Method ◽  
Root Extract ◽  
Bacterial Strains

Our research work was designed to investigate the curative and preventive effects of Carthamus oxycantha root extract against diarrhea and microorganisms. For the antibacterial experiment, the agar well diffusion method was used against standard bacteria Staphylococcus aureus, Escherichia coli, Pseudomonas aeroginosa, and Salmonella typhi, while for the assessment of antidiarrheal activity, castor oil and the magnesium sulfate-induced diarrhea method was used on albino, laboratory-bred (BALB/c) mice at a dose rate of 200 and 400 mg/kg (body weight, b.w) orally. The methanol extract of C. oxycantha significantly (p < 0.001) decreased the frequency of defecation, and wet stools in a dose depended on the manner of after receiving magnesium sulfate (2 g/kg (b.w)) and castor oil (1.0 mL/mice). Furthermore, the extract of C. oxycantha showed concentration-dependent antimicrobial properties against S. aureus followed by S. typhi, E. coli, and P. aeroginosa bacterial strains, with inhibitions ranging from 10.5–15 mm. These findings show significant results that C. oxycantha is effective as an antidiarrheal and antibacterial agent. However, further works are needed to establish its mode of action.

Characterization and thermal properties of chitosan/perlite nanocomposites

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Mehmet Doğan ◽  
Hatice Yüksel ◽  
Berna Koçer Kizilduman
Keyword(s):  
Antibacterial Activities ◽  
Diffusion Method ◽  
X Ray Diffraction ◽  
Scanning Calorimetry ◽  
Disk Diffusion Method ◽  
Force Microscopy ◽  
Chitosan Matrix ◽  
Atomic Force ◽  
Transmission Electron ◽  
Nano Size

Abstract In this study, chitosan/perlite nanocomposites were synthesized using the solvent casting method and then characterized using Fourier transform infrared spectroscopy, X-ray diffraction, optical contact angle, differential thermal analysis/thermogravimetry, differential scanning calorimetry, atomic force microscopy, transmission electron microscopy and Zetasizer NanoS devices. Perlite was determined to be dispersed in nano size and homogeneously in the chitosan matrix. Chitosan/perlite nanocomposite was generally more thermally stable compared to pure chitosan polymer. The fact that the amount of perlite in the nanocomposite increased showed that the hydrophilic properties of nanocomposites increased. In addition, antibacterial activities of the samples were investigated using the agar-disk diffusion method and hemocompatibility testing was also performed.

scholarly journals Antibacterial Activity of Silver Nanoparticles Synthesized by Bark Extract of Syzygium cumini

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Ram Prasad ◽  
Vyshnava Satyanarayana Swamy
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Cost Effective ◽  
Diffusion Method ◽  
Bark Extract ◽  
Force Microscopy ◽  
Atomic Force ◽  
Cost Effective Method ◽  
Uv Visible ◽  
Major Attention

The unique property of the silver nanoparticles having the antimicrobial activity drags the major attention towards the present nanotechnology. The environmentally nontoxic, ecofriendly, and cost-effective method that has been developed for the synthesis of silver nanoparticles using plant extracts creates the major research interest in the field of nanobiotechnology. The synthesized silver nanoparticles have been characterized by the UV-visible spectroscopy, atomic force microscopy (AFM), and scanning electron microscopy (SEM). Further, the antibacterial activity of silver nanoparticles was evaluated by well diffusion method, and it was found that the biogenic silver nanoparticles have antibacterial activity against Escherichia coli (ATCC 25922), Staphylococcus aureus (ATCC 29213), Pseudomonas aeruginosa (ATCC 27853), Azotobacter chroococcum WR 9, and Bacillus licheniformis (MTCC 9555).

scholarly journals The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

2019 ◽  
Vol 2 (1) ◽  
pp. 19
Author(s):  
Murni Halim
Keyword(s):  
Leaf Extract ◽  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Leaf Extracts ◽  
Natural Drugs ◽  
Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

scholarly journals Antibacterial Potential of Aloe weloensis (Aloeacea) Leaf Latex against Gram-Positive and Gram-Negative Bacteria Strains

2019 ◽  
Vol 2019 ◽  
pp. 1-4
Author(s):  
Yohannes Kelifa Emiru ◽  
Ebrahim Abdela Siraj ◽  
Tekleab Teka Teklehaimanot ◽  
Gedefaw Getnet Amare
Keyword(s):  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Maximum Activity ◽  
New Drugs ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative

Objective. To evaluate the antibacterial effects of the leaf latex of Aloe weloensis against infectious bacterial strains. Methods. The leaf latex of A. weloensis at different concentrations (400, 500, and 600 mg/ml) was evaluated for antibacterial activities using the disc diffusion method against some Gram-negative species such as Escherichia coli (ATCC 14700) and Pseudomonas aeruginosa (ATCC 35619) and Gram-positive such as Staphylococcus aureus (ATCC 50080) and Enterococcus fecalis (ATCC 4623). Results. The tested concentrations of the latex ranging between 400 and 600 mg·mL−1 showed significant antibacterial activity against bacterial strain. The highest dose (600 mg/ml) of A. weloensis leaf latex revealed the maximum activity (25.93 ± 0.066 inhibition zone) followed by the dose 500 mg/ml against S. aureus. The lowest antibacterial activity was observed by the concentration 400 mg/ml (5.03 ± 0.03) against E. coli. Conclusion. The results of the present investigation suggest that the leaf latex of A. weloensis can be used as potential leads to discover new drugs to control some bacterial infections.

scholarly journals Development of Castor Oil Based Poly(urethane-esteramide)/TiO2Nanocomposites as Anticorrosive and Antimicrobial Coatings

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Mohammed Rafi Shaik ◽  
Manawwer Alam ◽  
Naser M. Alandis
Keyword(s):  
Castor Oil ◽  
Tap Water ◽  
Antimicrobial Properties ◽  
Gel Permeation Chromatography ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Condensation Polymerization ◽  
Agar Disc ◽  
Bacteria And Fungi

Castor oil based polyesteramide (CPEA) resin has been successfully synthesized by the condensation polymerization of N-N-bis (2-hydroxyethyl) castor oil fatty amide (HECA) with terephthalic acid and further modified with different percentages of 7, 9, 11, and 13 wt.% of toluene-2,4-diisocyanate (TDI) to obtain poly(urethane-esteramide) (UCPEA), via addition polymerization. TiO2(0.1, 0.2, 0.3, 0.4, and 0.5 wt%) nanoparticles were dispersed in UCPEA resin. The structural elucidation of HECA, CPEA, and UCPEA has been carried out using FT-IR,1H-NMR, and13C-NMR spectroscopic techniques while physicochemical and physicomechanical properties were investigated by standard methods. Thermal stability and molecular weight of UCPEA have been assessed by thermogravimetric analysis (TGA) and gel permeation chromatography (GPC), respectively. Furthermore, the corrosion behavior of UCPEA coatings on mild steel has been investigated by potentiodynamic polarization measurements in different corrosive environments (3.5 wt% HCl, 5 wt% NaCl, 3.5 wt% NaOH, and tap water) at room temperature and surface analysis by scanning electron microscope (SEM) and energy dispersive X-ray (EDX). The antibacterial activities of the UCPEA were tested against bacteria and fungi by agar disc diffusion method. The results of this study have revealed that UCPEA nanocomposite coatings exhibit good physicomechanical, anticorrosion and antimicrobial properties, which can be safely used up to 200°C.

scholarly journals ANTIBACTERIAL ACTIVITY OF SOAP MADE FROM ESSENTIAL OILS AND COMMERCIAL SOAP SOLD IN THE LEBANESE MARKET

2021 ◽  
pp. e304
Author(s):  
Candy GERGES ◽  
Mohamad HADLA ◽  
Michele ELIALI ◽  
Martine RICHA ◽  
Nour Mammari ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Effective Measure ◽  
E Coli ◽  
Different Types ◽  
Commercial Soap ◽  
Zones Of Inhibition

Background: Thousands of people are dying as a result of infections caused by bacteria. Among the main routes of germ transmission are the hands, making hand hygiene very important in preventing the spread of pathogens and bacterial infection. Hand washing with soap and water is considered to be a simple and effective measure. Old soap manufacturers have long had traditional uses in the Lebanese community. Methods: The purpose of this study is to compare the antibacterial activity of oil-based soaps with commercial soap sold in the Lebanese market. Different types of herbal soap and antiseptics have been used in this study. Four bacterial strains were used: Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa, Escherichia coli and Enterobacter Spp. The antibacterial activities of these soaps were determined by the diffusion method of disks in agar medium. Results and Discussion: Statistical analysis of zones of inhibition showed that S. epidermidis, P. aeruginosa and Enterobacter Spp. were sensitive only to traditional oil-based soaps, which are Sage, Rose Mary and Cedar. In addition, S. aureus showed sensitivity to soaps comprised of essential oils as well as antibacterial synthetic soap, Dettol and Lifebuoy. On the other hand, E. coli showed resistance to all soaps. Soaps comprised of natural essential oils have shown antibacterial activity superior to so-called “Antibacterial” soaps. Conclusion: Based on this study, we can say that the use of soaps with essential oils might be the best option due to their organic origin as well as their antibacterial proved activities.

scholarly journals Exploration of Antioxidant, Antibacterial Activities of Green Synthesized Hesperidin Loaded PLGA Nanoparticles

2021 ◽  
Vol 11 (6) ◽  
pp. 14520-14528
Keyword(s):  
Enterobacter Aerogenes ◽  
Cost Effective ◽  
Antibacterial Activities ◽  
Plga Nanoparticles ◽  
Bacterial Strains ◽  
Antioxidants Activity ◽  
Good Ability ◽  
Potential Benefits ◽  
Greener Synthesis ◽  
Dose Dependent

To elaborate on the potential benefits of hesperidin loaded PLGA nanoparticles (HES-PLGA NPs), present investigation reports about the antioxidant, antimicrobial, and cytotoxic potential of hesperidin loaded PLGA nanoparticles (HES-PLGA-NPs). The antioxidant potential of Hesperidin and HES-PLGA-NPs was demonstrated by ATBS, DPPH, Hydroxy, Hydrogen peroxide, and superoxide radical showing the dose-dependent antioxidants activity. Both the test gained better potential with HES loaded PLGA nanoparticles than free HES. HES-PLGA-NPs also proved its antibacterial activity against tested bacterial strains, including Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, and Pseudomonas aeruginosa. HES-PLGA-NPs had a good ability to minimize biofilms' formation and a good to weak ability to degrade pre-formed biofilms. Thus, the bionanotherapy, which has been attempted using HES-PLGA-NPs would provide a promising scope as a potent bio compound that can be replaced for medical ailments in a cost-effective, greener synthesis.

scholarly journals Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Siti Samiyarsih ◽  
NUR FITRIANTO ◽  
ELLY PROKLAMASININGSIH ◽  
JUWARNO ◽  
JUNI SAFITRI MULJOWATI
Keyword(s):  
Antimicrobial Activity ◽  
Catharanthus Roseus ◽  
Methanol Extract ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
E Coli ◽  
Phytochemical Compounds ◽  
Dark Pink

Abstract. Samiyarsih S, Fitrianto N, Proklamasiningsih E, Juwarno, Muljowati JS. 2020. Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS. Biodiversitas 21: 1332-1344. Catharanthus roseus (L.) G is an important medicinal plant to evaluate the possibility of novel pharmaceuticals since most of the bacterial pathogens are developing resistance against antibiotics. This research aimed to determine the phytochemical diversity of methanol extract of eight cultivars of C. roseus and to evaluate for possible antimicrobial (antifungal and antibacterial) activities. It is the first research to compare phytochemicals and antimicrobial potential among C. roseus cultivars. The compound obtained was screened by Gas Chromatography-Mass Spectrometry (GC-MS) method. While agar-well disc diffusion method was employed to measure antimicrobial activity against Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Aspergillus niger. Based on GC-MS analysis, a total of 18 significantly different metabolite compounds. The abundances of phytochemical compounds (18 classes total) in each cultivar were Dark Pink (66%), Pink (50%), Purple Pink (44%), Pale Pink (27%), White (44%), Milky White (50%), Whitish pink (50%) and Pinkish Red (55%). The leaves extracts showed antimicrobial activity with inhibition zones ranging from 6.40-22.00 mm and 3.35-8.20 mm, respectively. The best antimicrobial activity against E. coli, S. aureus, A. flavus, and A. niger with the zone of inhibition 16.10±1.67 mm, 22.00±0.33 mm, 6.05±0.67 mm and 8.20±0.50 mm respectively by Dark Pink cultivar.

scholarly journals Extraction and Isolation of Compound from Roots of Kniphofia pumila, and Its Antibacterial Activities in Combination with Zinc Chloride

2020 ◽  
Vol 17 (7) ◽  
pp. 631-638
Author(s):  
Dele ABDISSA ◽  
Gezahegn FAYE ◽  
Melkamu FAYERA ◽  
Shiferaw DEMISSIE
Keyword(s):  
Antibacterial Activity ◽  
Crude Extract ◽  
Pathogenic Bacteria ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Spectroscopic Techniques ◽  
Bacterial Strains ◽  
Pure Compound ◽  
Standard Drug ◽  
Isolated Compound

Kniphofia pumila has been traditionally used for the treatment of different diseases in Oromiya regional state, western part of Ethiopia. Thus, the main objective of this study was to extract bioactive natural products from the roots of K. pumila and perform in vitro antibacterial activity tests against selected pathogens. Extraction of compound from the roots of K. pumila was carried out by maceration method at room temperature and its antibacterial activity was done using agar disc diffusion method. The crude extracts alone and along with ZnCl2 were tested against pathogenic bacteria Escherichia coli (ATCC 25722), Klebsiella pneumoniae subsp. pneumoniae (DSM 19613), Staphylococcus aureus (ATCC 25925) and Salmonella Typhimurium (ATCC 13311). Following its promising activity, the acetone crude extract that have shown better antibacterial activity was subjected to column chromatography for isolation of pure compound. Hence, one pure compound (GZ-1) was obtained from acetone crude extract. Meanwhile, the antibacterial activities of the isolated compound alone and in combination with ZnCl2 were also performed against all aforementioned bacterial strains. Then, isolated compound was characterized by using NMR spectroscopic techniques such as 1H-NMR, 13C-NMR and, 2D NMR, so as to establish its structure as 3’-acetyl-2’,6’-dihydroxy-4-methoxyphenyl-1,8-dihydroxy-3-methylanthraquinone, trivial name knipholone. Finally, GZ-1 was isolated from the roots of K. pumila and it was noted that the antibacterial activities of ZnCl2 in combination with crude extract as well as with isolated compound against E. coli (ATCC 25722), S. aureus (ATCC 25925) and S. Typhimurium (ATCC 13311) bacterial strains showed remarkable results which were greater than the antibacterial activity of the positive standard drug (gentamycin). To sum up, from the chromatographic isolation of roots acetone extract of K. pumila one pure compound (knipholone) was obtained. Its antibacterial activity was also evaluated in combination with ZnCl2 salt and alone.

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