scholarly journals Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS

2020 ◽  
Vol 21 (4) ◽  
Author(s):  
Siti Samiyarsih ◽  
NUR FITRIANTO ◽  
ELLY PROKLAMASININGSIH ◽  
JUWARNO ◽  
JUNI SAFITRI MULJOWATI
Keyword(s):  
Antimicrobial Activity ◽  
Catharanthus Roseus ◽  
Methanol Extract ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
E Coli ◽  
Phytochemical Compounds ◽  
Dark Pink

Abstract. Samiyarsih S, Fitrianto N, Proklamasiningsih E, Juwarno, Muljowati JS. 2020. Phytochemical diversity and antimicrobial properties of methanol extract of several cultivars of Catharanthus roseus using GC-MS. Biodiversitas 21: 1332-1344. Catharanthus roseus (L.) G is an important medicinal plant to evaluate the possibility of novel pharmaceuticals since most of the bacterial pathogens are developing resistance against antibiotics. This research aimed to determine the phytochemical diversity of methanol extract of eight cultivars of C. roseus and to evaluate for possible antimicrobial (antifungal and antibacterial) activities. It is the first research to compare phytochemicals and antimicrobial potential among C. roseus cultivars. The compound obtained was screened by Gas Chromatography-Mass Spectrometry (GC-MS) method. While agar-well disc diffusion method was employed to measure antimicrobial activity against Escherichia coli, Staphylococcus aureus, Aspergillus flavus and Aspergillus niger. Based on GC-MS analysis, a total of 18 significantly different metabolite compounds. The abundances of phytochemical compounds (18 classes total) in each cultivar were Dark Pink (66%), Pink (50%), Purple Pink (44%), Pale Pink (27%), White (44%), Milky White (50%), Whitish pink (50%) and Pinkish Red (55%). The leaves extracts showed antimicrobial activity with inhibition zones ranging from 6.40-22.00 mm and 3.35-8.20 mm, respectively. The best antimicrobial activity against E. coli, S. aureus, A. flavus, and A. niger with the zone of inhibition 16.10±1.67 mm, 22.00±0.33 mm, 6.05±0.67 mm and 8.20±0.50 mm respectively by Dark Pink cultivar.

Dicentra scandens (D.Don) Walp. - A Potential Source of Antimicrobial Agent

2017 ◽  
Vol 9 (01) ◽  
Author(s):  
Koirala Pramila ◽  
Singh Bimala
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Agent ◽  
Methanol Extract ◽  
Shigella Flexneri ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Methanol Extracts ◽  
Ms Analysis

Dicentra scandens (D.Don) Walp. locally called as ‘Jogi Lahara’ belongs to the Family Fumariaceae and is used in traditional medicine in Sikkim, a North Eastern state of India. The present study investigated the antimicrobial properties of aqueous and methanol extracts of Dicentra scandens (D.Don) Walp. against some test Gram negative and Gram positive bacteria. Using agar well diffusion method, aqueous and methanol extracts of roots of Dicentra scandens were tested against Escherichia coli (MTCC 1089), Klebsiella pneumoniae (MTCC 3384), Pseudomonas aeruginosa (MTCC 1034), Proteus vulgaris (MTCC 742), Salmonella typhi (MTCC 733), Shigella flexneri (MTCC 1457), Vibrio cholerae O139(MTCC 3906), Bacillus cereus (MTCC 6840) and Staphylococcus aureus (MTCC 7443). Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also evaluated. Promising antimicrobial activity was exhibited by methanol extract of Dicentra scandens. The methanol extract was further characterized by Gas Chromatography-Mass Spectrometry (GC-MS) analysis. The GC-MS analysis revealed 29 compounds and the major compounds detected were Protopine (53.78%) and Corydine (18.20%). Thus, the alkaloids are predominant phytoconstituents of the extract and could be attributed to its antimicrobial activity. The results of the present study indicate that, Dicentra scandens can be a source of potential antimicrobial agent and can be explored further for its therapeutic use.

Antimicrobial Activity of Photoactive Cerium Doped Zinc Oxide

2020 ◽  
Vol 307 ◽  
pp. 217-222 ◽  
Author(s):  
Maishara Syazrinni Rooshde ◽  
Wan Rafizah Wan Abdullah ◽  
Amie Zaidah Amran ◽  
Noradhiha Farahin Ibrahim ◽  
Fazilah Ariffin ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Uv Light ◽  
Light Exposure ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Gram Positive ◽  
E Coli ◽  
Antifouling Agent ◽  
Doped Zno ◽  
Zno Powder

Biofouling and biofilms exist as ubiquitous, undesirable accumulation of flora and fauna upon a given substrate when being immersed into an aquatic medium. Therefore, a novel antifouling based materials with the incorporation of nanotechnology has been developed for the prevention of biofouling in its initial stage through photocatalytic treatment. This study investigated the antimicrobial properties of photoactive Cerium (Ce) doped ZnO powder and explores its potential properties for future antifouling application. ZnO nanoparticles was doped with 0.4 mol% Ce was synthesized through the combination of modified citrate gelation technique and solid state sintering. The successful preparation of Ce doped ZnO was confirmed by XRD and SEM. The antimicrobial activity of Ce doped ZnO against E. coli and S. aureus was determined through antibacterial susceptibility test by agar well diffusion method whilst its photocatalytic inactivation efficiency against selected bacteria was analysed through photodegradation testing under UV light irradiation. The findings demonstrated that the synthesized Ce doped ZnO powder exhibited antibacterial effect against Gram-positive bacteria (S. aureus) and excellent photocatalytic efficiency to inactivate both Gram-negative (E. coli) and Gram-positive (S. aureus). 2 g/L of Ce doped ZnO catalyzed the 100% disinfection of both bacteria in 180 min of UV light exposure. Thus, this proved that Ce doped ZnO powder has the potential as efficient antifouling agent.

scholarly journals Phytochemical Investigation and Antimicrobial Activity of Hexane, Ethyl Acetate and Methanol Fractions from Stem Bark of Icacina Trichantha Oliv. (Icacinaceae)

2020 ◽  
Vol 7 (1) ◽  
pp. 7-12
Author(s):  
Ojah Emmanuel Onah ◽  
Kachi Jolly Babangida
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Antimicrobial Properties ◽  
Ethyl Acetate Fraction ◽  
Hexane Fraction ◽  
Diffusion Method ◽  
Economic Losses ◽  
Phytochemical Screening ◽  
E Coli ◽  
Micro Organisms

Background: Micro-organisms are responsible for the transmission of a large number of diseases. It is hard to comprehend the amount of diseases, deaths and economic losses caused by micro-organisms alone. Plants are good sources of eco-friendly and readily available antimicrobial agents. Purpose: The aim of this study was to evaluate the chemical constituents and antimicrobial characteristics of three fractions from ethnomedicinal Icacina trichantha. Oliv. (Icacinaceae).Methods: Methanol extract from Icacina trichantha. Oliv was obtained by maceration and fractionated successively using hexane, and ethyl acetate. The antimicrobial properties of Icacina trichantha. Oliv was assessed using agar cup diffusion method on MRSA, P. aeruginosa, S. typhi, C. krusei, S. dysenteriae, S. pyrogenes, E. coli, K. pneumoniae, C. albicans, and C. tropicalis. Phytochemical screening on fractions was also evaluated using standard methods.Results: Phytochemical screening on fractions revealed the presence of saponins, alkaloids, steroids, tannins, and glycosides. Agar diffusion assay on fractions showed growth inhibitory effect on all the organisms except P. aeruginosa, S. typhi, and C. tropicalis. The MIC revealed that n-hexane fraction was active against MRSA, S. pyrogenes, E. coli, K. pneumonia, C. albicans and C. krusei at 10 mg/mL while S. dysenteriae was active at 5 mg/mL. The ethyl acetate fraction was active against all the organisms at a concentration of 5 mg/mL except P.aeruginosa, S.typhi and C.tropicalis. Methanol fraction showed activity of 5 mg/mL against MRSA, S. pyrogenes, E. coli, S. dysenteriae, C. albicans and C. krusei except for K. pneumoniae with activity at 10 mg/mL. Minimum bactericidal concentration/fungicidal concentration MBC/MFC evaluated on the n-hexane fraction revealed that MRSA, S. pyrogenes, E. coli, K. pneumoniae, S. dysenteriae, C. albicans, and C. krusei were activeat 20 mg/mL, while the ethyl acetate fraction had MBC/MFC of 10 mg/mL against all the organisms except P. aeruginosa, S. typhi, C. tropicalis. Methanol extract had MBC/MFC of 10 mg/mL against MSRA, E.coli and S. dysenteriae whereas S. pyrogenes, K. pneumoniae, C. albicans and C. krusei had MBC/MFC at 20 mg/mL.Conclusion: Icacina trichantha. Oliv. contain constituents with concentration dependent antimicrobial properties based on type of organism. The plant could be useful in the prevention and treatment of multi-resistant disease causing microorganisms.

scholarly journals Comparative Phytochemical and Antimicrobial Properties of Two Cultivars of Catharanthus roseus L. {G.} Don on Escherichia coli and Candida albicans

2021 ◽  
Vol 3 (2) ◽  
pp. 44-48
Author(s):  
David Mutisya Musyimi ◽  
Marble Namarobe Namnabah
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Bioactive Compounds ◽  
Catharanthus Roseus ◽  
Antimicrobial Properties ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Leaf Extracts ◽  
Synthetic Drugs

Medicinal plants have served as sources of medicine to treat and suppress the diseases, because many pathogens are gaining resistance to the current synthetic drugs. In addition, high cost and adverse side effects are commonly associated with popular Synthetic drugs. Therefore, there is need for continuous search for new drugs in order to overcome this emerging resistance. Plants synthesize bioactive compounds which are of great potential in agriculture, antimicrobial and anti-insect activity. The concentration of bioactive compounds in each plant species depends on the environmental conditions, age of the plant, relative humidity of harvested materials and method of extraction. Little is known on the phytochemical and antimicrobial potential of Alba and Rosea cultivars of Catharanthus roseus ethanol extracts. The leaves of Alba and Rosea cultivars were investigated for their phytochemical and antimicrobial properties. The study was conducted at Maseno University, Kenya. Plant Leaves were collected around Maseno University. Leaves of Alba and Rosea cultivars of Catharanthus roseus were air-dried in the shade, thereafter crushed into powder and ethanol extraction done using the Rotary evaporator. Antimicrobial activity of the pathogenic microorganisms was Candida albicans and Escherichia coli.  Disc diffusion method was used for antimicrobial tests. concentrations of ethanol leaf extracts consisted of 2.5, 5and 7.5 mg/mL with three replications.  Data on growth inhibition were subjected to analysis of variance (ANOVA) . Phytochemical analysis revealed the presence of tannins, flavonoids, terpenoids, saponins, alkaloids and phenols except steroids and glycosides. The ethanol leaf extracts were active against Candida albicans and and Escherichia coli. Alba leaves extracts showed higher inhibitory zones compared to Rosea leaves. The observed differences in antimicrobial activity could be due to differences in cell wall synthesis, structure and composition. The results of present study further confirm the use of these plants traditionally for the treatment of different ailments.

scholarly journals In vitro antibacterial activity and potential applications in food of sea urchin (Diadema setosum) from Cape of Palette, South Sulawesi

Food Research ◽  
2020 ◽  
Vol 4 (6) ◽  
pp. 2139-2146
Author(s):  
M. Yusuf ◽  
U.A. Fitriani Nur ◽  
A. Rifai
Keyword(s):  
Antibacterial Activity ◽  
Sea Urchin ◽  
Methanol Extract ◽  
Marine Invertebrates ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Ultrasound Assisted Extraction ◽  
E Coli ◽  
Diadema Setosum

Marine invertebrates in support of his life's defence of sea predatory use an innate immune mechanism, namely the cellular component hemocytes by secreting the dissolved antimicrobial and cytotoxic substances. It shows that marine invertebrates are potential sources and promising antimicrobial compounds. This research was aimed at determining the antibacterial activity of sea urchin (Diadema setosum) extract against some bacterial isolates and its application to foodstuffs. The gonad and shell of sea urchin extracted by methanol and ethyl acetate and then separated by ultrasound-assisted extraction. Screening of antibacterial compound use gas chromatography-mass spectrometry (GC-MS), and disc diffusion method was followed to determine the antimicrobial activity against Salmonella, Escherichia coli and Staphylococcus aureus. The results of this study showed antibacterial activity against one or more strains. The gonad of sea urchin from methanol extract exhibited significant inhibitory effect and effective against Salmonella, E. coli and S. aureus. Majority of gonad and shell of sea urchin extract showed antibacterial activity against the tested strains. However, gonad of methanol extract was found to be inhibiting microorganisms gram-negative (E. coli) and gram-positive (Salmonella and S. aureus). Gonad extract can be a good source of antibacterial agents.

scholarly journals Isolation and screening marine fungi with antimicrobial activity from samples collected in Nha Trang bay, VietNam

2018 ◽  
Vol 16 (1) ◽  
pp. 181-187
Author(s):  
Phan Thi Hoai Trinh ◽  
Phi Quyet Tien ◽  
Ngo Thi Duy Ngoc ◽  
Bui Minh Ly ◽  
Tran Thi Thanh Van
Keyword(s):  
Antimicrobial Activity ◽  
Marine Fungi ◽  
Antimicrobial Properties ◽  
Biologically Active ◽  
Microbial Pathogens ◽  
Diffusion Method ◽  
Disc Diffusion Method ◽  
E Coli ◽  
Nha Trang Bay ◽  
Complex Ecosystem

The marine environment is an extremely complex ecosystem and contains a broad spectrum of fungal diversity. Marine fungi have been shown to be tremendous sources for new and biologically active secondary metabolites. The present study aims to isolate and screen antimicrobial properties of 100 fungus strains from different marine sources including seaweeds, soft corals, sponges and sediment collected at Nha Trang Bay, Vietnam. In preliminary experiments, the crude extracts of these fungal isolates with ethyl acetate were screened for their antimicrobial activity against the human microbial pathogens including Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853, Listeria monocytogenes ATCC 19111, Streptococcus faecalis ATCC 19433 and Candida albicans ATCC 10231 by the disc diffusion method. Among the 100 isolates, 59 strains exhibited antimicrobial activity against at least two tested pathogens, that 57% against S. aureus, 50% against L. monocytogenes, 49% against B. cereus, 45% against S. faecalis, 7% against E. coli, 5% against C. albicans, and only 2% against P. aeruginosa. The present study has revealed the presence of high numbers of marine fungi from Nha Trang waters having antimicrobial properties and they need to be investigated further for natural bioactive products.

GC-MS analysis of Phytochemical compounds in different extracts of Curculigo orchiodes

2021 ◽  
pp. 4355-4360
Author(s):  
R. Sai Nandhini ◽  
R. Nirmala Nithya ◽  
Vidhya K.
Keyword(s):  
Methanol Extract ◽  
Ethanol Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Zone Of Inhibition ◽  
Study Results ◽  
Phytochemical Compounds ◽  
Ethanol Extracts ◽  
Vinyl Phenol

The present study investigates the presence of phytochemical compounds in the ethanol and methanol extract of Curculigo orchiodes, it was elucidated using Gas chromatography-Mass spectrometry. Qualitative phytochemical compounds of both extracts reveal the presence of flavonoids, tannins, phenol, protein, cardiac glycosides, terpenoids, and carbohydrate and have a higher level compounds in ethanol extracts of C.orchiodes. Fifteen bioactive phytocompounds were identified in the ethanol and methanol extract of C.orchiodes. GCMS reveal the presence of 2-Furanmethanol, Cyclopentanone, 2-Methyl, Formic acid, 2-Propenyl ester, 2,5-Furandione, 3-Methyl, phenol 3-Methoxy-, 2-Methoxy-4-vinyl phenol, 1,2-Benzenediol, 3-methoxy-, n-Hexadecanoic acid and phytol which has medicinal property such as antibacterial, antiviral, anticancer and antioxidant. C.orchiodes was assayed for antibacterial activity against the tested microorganisms such as Escherichia Coli, Klebsiella Pneumonia, Pseudomonas aeruginosa using well diffusion method. Ethanol extract show the highest zone of inhibition ranging from 3.4±0.200 to 5.28±0.350 compared with standard antibiotics. From this study, results highlight that the ethanol extract of C.orchiodes will be a valuable source for the production of herbal medicine and maintaining human health.

scholarly journals Chemical Composition and Antimicrobial Activity of Essential Oils of Genista ulicina and G. vepres

2010 ◽  
Vol 5 (5) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Takia Lograda ◽  
Adel Nadjib Chaker ◽  
Jean Claude Chalchat ◽  
Messaoud Ramdani ◽  
Hafsa Silini ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Aerial Parts ◽  
Total Oil ◽  
And Staphylococcus Aureus

The hydrodistilled oils from the aerial parts of Genista ulicina Spach. and G. vepres Pomel., which are endemic to Algeria, were analyzed by gas chromatography-mass spectrometry (GC-MS). In the oil of G. ulicina, 41 compounds were identified representing 90.8% of the total oil, and in G. vepres, 61 compounds representing 84.5% of the total oil. The analyses showed that the major constituents of the oils were lauric acid (14.3% – 8.5%), myristic acid (11.5% – 5%), linoleic acid (3.1% –11.7%) and palmitic acid (18.6% – 26.4%). Using a diffusion method, the oils showed significant antibacterial activities against Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853) and Staphylococcus aureus (ATCC 25923).

scholarly journals Antimicrobial Activity of the Essential Oil Obtained from the Seed and Oleo-Gum-Resin of Ferula assa-foetida Against Oral Pathogens

2019 ◽  
Author(s):  
Alireza Daneshkazemi ◽  
Hengameh Zandi ◽  
Abdolrahim Davari ◽  
Mahmood Vakili ◽  
Majid Emtiazi ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Oral Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Streptococcus Sobrinus ◽  
Disk Diffusion Method

Objectives: The objective of this study was to evaluate the antimicrobial activity of the essential oil obtained from oleo-gum-resin and seeds of Ferula assa-foetida. Materials and Methods: Ferula assa-foetida plants were collected from Tabas, Yazd Province, Iran, during summer 2017. Then, essential oils were obtained from its seeds and oleo-gum-resin using hydrodistillation. Gas chromatography-mass spectrometry (GC-MS) test was performed to determine the contents of the essential oils. Four different concentrations of each oil were prepared (2.5, 5, 10, and 20 μg/ml), and the antimicrobial activity of each dose against four oral bacteria (Streptococcus mutans, Streptococcus sobrinus, Streptococcus sanguis, Streptococcus salivarius, and Lactobacillus rhamnosus) was evaluated using the disk diffusion method. The data were analyzed using analysis of variance (ANOVA) and Kruskal-Wallis test in SPSS 17 software. Results: The GC-MS findings exhibited that the main compounds found in essential oils yielded from the seeds and oleo gum resin were (Z) -1-propenyl sec-butyl disulfide and (E) -1-propenyl sec-butyl disulfide. Ferula assa-foetida plant showed a significant antimicrobial effect (P<0.05). The essential oil from Ferula assa-foetida oleo-gum-resin had significantly stronger antibacterial properties compared to the essential oil from Ferula assa-foetida seeds (P<0.001). Both essential oils showed antibacterial properties similar to that of Chlorhexidine. The growth inhibition zone was significantly dependent on the essential oil concentration for all bacteria (P<0.05). Conclusion: Our study revealed that essential oils from seeds and oleo-gum-resin of Ferula assa-foetida have antimicrobial properties. More laboratory studies are required to reach a definitive conclusion.

scholarly journals Evaluation of Extracts of Leaves of Crinum jagus for Antimicrobial Properties

2020 ◽  
Vol 24 (7) ◽  
pp. 1197-1201
Author(s):  
T.T. Alawode ◽  
L. Lajide ◽  
B.J. Owolabi ◽  
M.T. Olaleye
Keyword(s):  
Inhibitory Concentration ◽  
Methanol Extract ◽  
Antimicrobial Properties ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Diffusion Method ◽  
Penicillium Notatum ◽  
E Coli ◽  
Agar Diffusion ◽  
Zones Of Inhibition

Plants have been used in ethno-medicine for ages in the treatment of various diseases. In the current study, the leaves of C. jagus are investigated  for antimicrobial activities. The leaves were dried and extracted successively with hexane, ethylacetate and methanol. The concentrated extracts  were screened for activity against Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi, Klebisidlae pneumonae, Candida albicans, Aspergillus niger, Penicillium notatum and Rhizopus stolonifer at concentrations between 6.25 and 200 mg/ml using the agar diffusion method. The Minimum Inhibitory Concentration (MIC) was also determined. The percentage yields obtained were 0.92 %, 1.20 %  and 25.2 % for the hexane, ethylacetate and methanol extracts respectively. The zones of inhibition of the organisms by the extracts generally increased with the concentrations. The methanol extract showed the best activity of the three extracts tested. The methanol extract had values ranging between 20 mm and 26 mm against the bacteria at 200 mg/ml. This extract also showed values between 18 mm and 20 mm against the  fungi at 200 mg/ml. The lowest MICs values (of 2.5 mg/ml) were obtained against S. aureus, E. coli, B. subtilis and S. typhi. The polar constituents in  the leaves of the plant are likely responsible for the antimicrobial properties observed. None of the extracts showed activity comparable to those of the standard drugs, however the methanol extract showed some potential as a source of antimicrobial compounds. Keywords: Crinum jagus, antimicrobial, Agar diffusion, MIC

Sign in / Sign up

Export Citation Format

Share Document