Red dragon fruit juice in reducing ros levels and insulin resistance In rats with type 2 diabetes mellitus model

2021 ◽  
Vol 10 (1) ◽  
pp. 6-14
Author(s):  
Mahendri Deayu Putri ◽  
Budiyanti Wiboworini ◽  
Paramasari Dirgahayu
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Fruit Juice ◽  
Intervention Group ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Positive Group ◽  
Dragon Fruit

Background: The peel of red dragon fruit (Hylocereus polyrhizus) had been proven to have a total polyphenol content and total flavonoids 2 to 3 times more than its flesh. These components could reduce oxidative stress and maintain the function of pancreatic beta cells, which could affect blood sugar levels.Objectives: This study aimed to test the red dragon fruit juice using peel and flesh to reduce oxidative stress and insulin resistance in T2DM model rats.Materials and Methods: This study was a true experimental study with a randomized controlled trial, with a Matching Pretest Post-test Control Group Design. We used 21 white male rats (Rattus norvegicus) Wistar strain which was divided into three groups: (P1) negative control group (induced Streptozotocin + Nicotinamide induction), (P2) positive control group (given Streptozotocin + Nicotinamide and given Metformin HCl induction 0,9 mg/kg BW, and (P3) Red Dragon fruit group (induced Streptozotocin + Nicotinamide and given Red Dragon Fruit juice 3.6 ml / 200 g BW / day given for 14 days. The data were analyzed using a one-way ANOVA test, paired t-test, and Post Hoc.Results: After 14 days of intervention, the average HOMA-IR levels were as follows: negative control group (Mean=8.32; SD=0.26), positive group (Mean 4.89; SD=0.29), and the Red Dragon Fruit intervention group (Mean=4.65; SD=0.30). The average MDA levels were as follows: control group (Mean = 9.08; SD = 0.68), positive group (Mean=3.34;SD=0.22), and the red dragon fruit intervention group (Mean = 3.05; SD = 0.47). Both the Metformin group and the Red Dragon Fruit group had low HOMA-IR and MDA levels compared to the negative control group.Conclusions: When administered alone, red dragon fruit and metformin effectively reduced HOMA-IR and MDA levels in rats with type 2 DM. Red dragon fruit can be used as an alternative to metformin because of its effectiveness in reducing plasma HOMA-IR and MDA.

Effect of a Polyphenol-Rich Extract from Aloe vera Gel on Experimentally Induced Insulin Resistance in Mice

2007 ◽  
Vol 35 (06) ◽  
pp. 1037-1046 ◽  
Author(s):  
Yolanda Y. Pérez ◽  
Enrique Jiménez-Ferrer ◽  
Alejandro Zamilpa ◽  
Marcelino Hernández-Valencia ◽  
Francisco J. Alarcón-Aguilar ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Aloe Vera ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Insulin Tolerance ◽  
Aloe Vera Gel ◽  
Experimentally Induced

Insulin resistance, which precedes type 2 diabetes mellitus (T2DM), is a widespread pathology associated with the metabolic syndrome, myocardial ischemia, and hypertension. Finding an adequate treatment for this pathology is an important goal in medicine. The purpose of the present research was to investigate the effect of an extract from Aloe vera gel containing a high concentration of polyphenols on experimentally induced insulin resistance in mice. A polyphenol-rich Aloe vera extract (350 mg/kg) with known concentrations of aloin (181.7 mg/g) and aloe-emodin (3.6 mg/g) was administered orally for a period of 4 weeks to insulin resistant ICR mice. Pioglitazone (50 mg/kg) and bi-distilled water were used as positive and negative controls respectively. Body weight, food intake, and plasma concentrations of insulin and glucose were measured and insulin tolerance tests were performed. The insulin resistance value was calculated using the homeostasis model assessment for insulin resistance (HOMA-IR) formula. Results showed that the polyphenol-rich extract from Aloe vera was able to decrease significantly both body weight ( p < 0.008) and blood glucose levels ( p < 0.005) and to protect animals against unfavorable results on HOMA-IR, which was observed in the negative control group. The highest glucose levels during the insulin tolerance curve test were in the negative control group when compared to the Aloe vera extract and pioglitazone treated mice ( p < 0.05). In conclusion, Aloe vera gel could be effective for the control of insulin resistance.

scholarly journals Protective Effect of Combination Commercial Black Seed Oil (Nigella sativa) and Honey Against Cisplatin-Induced Hepatotoxicity in Rats

2020 ◽  
Vol 1 (2) ◽  
pp. 43
Author(s):  
Andri Muhrim Siddiq ◽  
Muhammad In'am Ilmiawan ◽  
Mitra Handini
Keyword(s):  
Oxidative Stress ◽  
Protective Effect ◽  
Rat Liver ◽  
Liver Tissue ◽  
Seed Oil ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Black Seed ◽  
Black Seed Oil

Background: The chemotherapeutic use of cisplatin (CP) is restricted because of its hepatotoxicity induced by oxidative stress. Malondialdehyde (MDA) is a secondary product of lipid peroxidation as a biomarker of oxidative stress. Individual administration of black seed oil (BSO) or honey (H) demonstrated hepatoprotective effect in rats. Interaction of both substances when administrated as combination can be evaluated using combination index (CI) to quantitatively depict synergism (CI<1), additive (CI=1) and antagonism effect (CI>1). Objective: to know the combination effect of BSO and honey on rat liver tissue given CP exposure. Methods: This study used 30 rats were divided into 10 groups. Normal group (N); Negative control group (NC); P1-P4 groups were administerated BSO (1 and 2 mL/kg) and honey (3.7 and 7.4 mL/kg); P5-P8 groups were combination of BSO and H. P1-P8 groups were given BSO and honey orally for 21 days. On the 18th day, NC and P1-P8 groups were given CP 8 mg/kg intraperitoneally, while the N group was given NaCl 0.9% 1 mL/kg intraperitoneally. Result: Malondialdehyde (MDA) levels were found to be lower in P1-P8 groups compared to negative control group and P6 and P7 groups have levels equivalent to MDA levels of normal control group (p > 0.05). Conclusion: Combination of BSO and honey provides a protective effect on cisplatin-induced rat liver tissue damage indicated by reduced MDA levels, but all combination group showed antagonism effect.

scholarly journals Beneficial Effects of Poplar Buds on Hyperglycemia, Dyslipidemia, Oxidative Stress, and Inflammation in Streptozotocin-Induced Type-2 Diabetes

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Shiqin Peng ◽  
Ping Wei ◽  
Qun Lu ◽  
Rui Liu ◽  
Yue Ding ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Blood Glucose ◽  
Crude Extract ◽  
Liver Homogenate ◽  
Control Group ◽  
Diabetic Mice ◽  
Model Control

The effects of propolis on blood glucose regulation and the alleviation of various complications caused by diabetes have been widely studied. The main source of propolis in the northern temperate zone is poplar buds. However, there is limited research on the antidiabetic activity of poplar buds. In order to evaluate the effect of poplar buds on type-2 diabetes, crude extract and 50% fraction of poplar buds were used to feed streptozotocin-induced type-2 diabetic mice. The results showed that 50% fraction could increase insulin sensitivity and reduce insulin resistance, as well as decrease the levels of fasting blood glucose, glycated hemoglobin, and glycosylated serum proteins in diabetic mice. Compared with the model control group, the 50% fraction-treated group showed significant decreases of malondialdehyde (MDA) and increases of superoxide dismutase (SOD) in serum and liver homogenate. Moreover, 50% fraction could significantly decrease total cholesterol (TC), alleviate abnormal lipid metabolism, and enhance antioxidant capacity in the serum. For inflammatory factors, feeding of 50% fraction could also reduce the levels of interleukin 6 (IL-6), tumor necrosis factorα(TNF-α), monocyte chemotactic protein 1 (MCP-1), and cyclooxygenase-2 (COX-2) in liver homogenate. Taken together, our results suggest that crude extract and 50% fraction of poplar buds, particularly the latter, can decrease blood glucose levels and insulin resistance, and 50% fraction can significantly relieve dyslipidemia, oxidative stress, and inflammation caused by type-2 diabetes.

scholarly journals Effects of Metformin in Combination with a Herbal Capsule (Glucoblock) on Insulin Resistance and Oxidative Stress Index in Type 2 Diabetic Rats

2021 ◽  
pp. 18-25
Author(s):  
Ojoye N. Briggs ◽  
Kemzi N. Elechi-amadi ◽  
Justice C. Ohaka ◽  
Edna O. Nwachuku ◽  
Bartimaeus S. Ebirien-agana
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Stress Index ◽  
Control Group ◽  
Oxidative Stress Index ◽  
Treatment Groups ◽  
And Oxidative Stress

Aim: This study evaluated the effects of metformin in combination with a herbal capsule (glucoblock) on insulin resistance and oxidative stress index in type 2 diabetic rats. Methodology: A total of 35 male Wistar albino rats weighing between 120-220 g were used for this study. The rats were placed on high fat diet, and diabetes was induced by a single intraperitoneal injection of freshly prepared streptozotocin (STZ) (45 mg/kg body wt). Fasting plasma glucose (FPG) was determined using the glucose oxidase method. Fasting plasma insulin (FPI), total oxidant status (TOS), total antioxidant status (TAS) and superoxide dismutase (SOD) levels were quantitatively determined by a rat-specific sandwich-enzyme linked immunosorbent assay (ELISA) method. Insulin resistance (IR) was determined using the homeostatic model assessment for insulin resistance (HOMA-IR) method. Oxidative stress index (OSI) was determined by the ratio of TOS to TAS. Phytochemical analysis on the herbal capsule was done using classical methods. Results: The results revealed the presence of alkaloids (100.31μg/mg), flavonoids (131.45μg/mg), cardiac glycosides (55.93μg/mg) and saponins (61.47μg/mg) in the herbal drug glucoblock. The results showed significantly lower FPG levels in the treatment groups when compared to the diabetic control. Group 3 administered metformin had significantly higher FPG levels compared to the negative control. Group 4 administered the herbal drug glucoblock and group 5 administered a combination of metformin and glucoblock, showed no significant differences in FPG levels when compared to the negative control. The diabetic control had significantly higher FPI levels compared to the negative control and treatment groups. The treatment groups showed no significant differences in FPI levels when compared to the negative control. HOMA-IR was significantly higher in the diabetic control compared to the negative control and treatment groups. Also, HOMA-IR values in the treatment groups showed no significant difference compared to the negative control except for group 3 (metformin), that was significantly higher than the negative control. SOD was significantly lower in the diabetic control, compared to the negative control and treatment groups. There were no significant differences in SOD levels in the treatment groups compared to the negative control. TOS levels in the negative control group and treatment groups were significantly lower, compared to the diabetic control. TAS was significantly lower in the diabetic control and treatment groups compared to the negative control. OSI in the diabetic control was significantly higher, compared to the negative control and treatment groups. Also, the treatment groups had significantly higher OSI compared to the negative control. Conclusion: High fat diet and streptozotocin induction produced significant insulin resistance and oxidative stress in the diabetic rats. Glucoblock was more effective in reducing insulin resistance compared to metformin. The combination showed synergistic drug-herb reaction as glucoblock potentiated the actions of metformin. Both showed antioxidant potential but were not effective in lowering oxidative stress to normal levels. There is need to incorporate antioxidant therapy in the treatment protocol for diabetes mellitus.

scholarly journals Chlorogenic acid prevents alcohol-induced brain damage in neonatal rat

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Zikang Guo ◽  
Jiang Li
Keyword(s):  
Oxidative Stress ◽  
Cognitive Function ◽  
Chlorogenic Acid ◽  
Inflammatory Mediators ◽  
Caspase 3 ◽  
Negative Control Group ◽  
Treated Group ◽  
Negative Control ◽  
Neonatal Rat ◽  
Control Group

AbstractThe present investigation evaluates the neuroprotective effect of chlorogenic acid (CA) in alcohol-induced brain damage in neonatal rats. Ethanol (12 % v/v, 5 g/kg) was administered orally in the wistar rat pups on postnatal days (PD) 7-9. Chlorogenic acid (100 and 200 mg/kg, p.o.) was administered continuously from PD 6 to 28. Cognitive function was estimated by Morris water maze (MWM) test. However, activity of acetylcholinesterase, inflammatory mediators, parameters of oxidative stress and activity of caspase-3 enzyme was estimated in the tissue homogenate of cerebral cortex and hippocampus of ethanol-exposed pups. It has been observed that treatment with CA attenuates the altered cognitive function in ethanol-exposed pups. There was a significant decrease in the activity of acetylcholinesterase in the CA treated group compared to the negative control group. However, treatment with CA significantly ameliorates the increased oxidative stress and concentration of inflammatory mediators in the brain tissues of ethanol-exposed pups. Activity of caspase-3 enzyme was also found significantly decreased in the CA treated group compared to the negative control group. The present study concludes that CA attenuates the neuronal damage induced in alcohol exposed neonatal rat by decreasing the apoptosis of neuronal cells.

scholarly journals Efek Protektif Jus Buah Nanas (Ananas Comosus (l.) Merr.) Terhadap Kadar Enzim SGOT dan SGPT Mencit (Mus Musculus L) Jantan Yang Dipapari Tuak Lokal Lombok

2020 ◽  
Vol 13 (1) ◽  
pp. 38-45
Author(s):  
Ari Khusuma
Keyword(s):  
Oxidative Stress ◽  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Palm Wine ◽  
White Rats ◽  
Alcoholic Drinks

Traditional alcoholic drinks are made traditionally or conventionally and simply packed. One of the traditional alcoholic drinks in Indonesia and commonly consumed is Tuak (Palm wine). Several indigenous communities in Lombok have fermented drinks popularly known as Tuak or Arak. Palm sap which is the basic ingredient in making palm wine containing alcohol. The most common liver diseases due to alcohol abuse include fatty liver, alcoholic hepatitis and liver cirrhosis. Ethanol metabolism in liver cells can cause an increase in the production of free radicals with various mechanisms so that oxidative stress will damage liver tissue. The supply of pineapple can prevent and repair liver damage due to oxidative stress processes, especially those caused by alcohol. Markers of liver damage are SGOT and SGPT enzymes. This research is a true experiment. The total number of experimental animals used was 21 individuals, negative control rats were only given standard feed, positive control rats were given palm wine without pineapple extract and treatment group were given palm wine and pineapple extract. Palm wine and pineapple were given together with standard feed for 14 days and on day 15th, the blood of the rats was taken to check the levels of SGOT and blood SGPT. The results showed the average SGOT enzyme levels in the negative control group 117.85 U / L, in the positive control group 126.14 U / L, and the treatment group 110.71 U / L. ANOVA test shows SGOT levels of white rats have significant differences in the experiments conducted (p <0.05). Examination of SGPT enzyme levels in the negative control group obtained an average yield of 25.42 U / L, positive control 30.42 U / L, and treatment 29.14 U / L. The ANOVA test results of SGPT levels in white rats show significant differences in the experiments conducted (p <0.05).  

scholarly journals Kebar Grass Extract (Biophitum Petersianum) Increases Diameter Of The Seminiferous Tubules Of Diabetic Mice

2021 ◽  
Vol 17 (1) ◽  
pp. 41-48
Author(s):  
Faraida Arvilla
Keyword(s):  
Oxidative Stress ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Seminiferous Tubules ◽  
Control Group ◽  
P Value ◽  
Diabetic Mice ◽  
Significant Difference ◽  
Stress Oxidative

Diabetes is a metabolic disease that can affect the male reproductive system. The condition of hyperglycemia will increase the production of ROS, causing oxidative stress. Oxidative stress causes microangiopathy, triggers cell apoptosis, interferes with spermatogenesis, thereby affecting the diameter of the seminiferous tubules. Kebar grass contains of flavonoid and vitamin E which can be used to reduce ROS reactions and inhibit oxidative stress. This research was to prove that kebar grass extract can increase diameter of the seminiferous tubules of diabetic mice. Total of 30 samples were divided into 5 groups: negative control group (K-) was given CMC-Na, positive control group (K+) was given metformin 2mg/kg, groups P1, P2 and P3 received 2mg/kg metformin and kebar grass extract with different dosages (67.5mg/kg, 135mg/kg and 270mg/kg). The treatment lasted for 35 days. On the 36th day the mice were sacrificed and the testes were taken, then histological observations were made with HE staining to measure the diameter of tubules. The result showed that there was a significant difference in the diameter of seminiferous tubules with p value=0,000. Kebar grass extract can increase the diameter of seminiferous tubules of diabetic mice.

scholarly journals The Effect Of Giving Red Dragon Fruit (Hylocereus polyrhizus) Extract On Spermatozo Motility In White Rats (Rattus norvegicus) Wistar Line With High Fat Induction

2020 ◽  
Vol 6 (3) ◽  
pp. 51-62
Author(s):  
Kirani Nuranisa ◽  
Cut Fauziah ◽  
Yuli Suciati
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
University Education ◽  
Control Group ◽  
High Fat ◽  
Fruit Extract ◽  
White Rats ◽  
Group I ◽  
Dragon Fruit ◽  
Hylocereus Polyrhizus

Hyperlipidemia is a condition when high level of blood lipids and causes morphologicalchanges in spermatozoa. Red dragon fruit (Hylocereus Polyrhizus) is one of the plantsas a source of antioxidants and has unsaturated fat content that is needed in thespermatozoa maturation process. The aims of this the study is to determine the benefitsof red dragon fruit extract on the motility of white rat spermatozoa (Rattus norvegius)induced by high-fat diet. The study is design by true experimental research andresearch only testing group design. This research conducted in the Pharmacology andTherapy Laboratory, Padjajaran University Education Hospital, in January 2020 usingwhite rats, male . ± 8 weeks wistar strains weighing ± 200-250. The study sample wasdivided into several groups such group I as a control, group II as a negative control,group III as positive group with simvastatin dose 0.72 mg / day, group IV as researchgroup 1 using red dragon fruit extract dose of 60 mg / 200 grBB / day for 52 days, andgroup V as group 2 therapy using dragon fruit extract dose of 60 mg / 200grBB / dayfor 104 days. The results showed that the mean percentage of motility of ratspermatozoa increased in the relief group with the supplement of red dragon fruitextract by dosing of 60 mg / 200 grBB / day for 104 days, or 51, 2%. This studyconclude that red dragon fruit has a superior effectivity better than simvastatin inincreasing the motility of spermatozoa.

Evaluation and correlation of oxidative stress and haemato-biochemical observations in horses with natural patent and latent trypanosomosis in Punjab state of India

2018 ◽  
Vol 63 (4) ◽  
pp. 733-743 ◽  
Author(s):  
Rahul Parashar ◽  
Lachhman Das Singla ◽  
Mayank Gupta ◽  
Suresh Kumar Sharma
Keyword(s):  
Oxidative Stress ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Group Ii ◽  
Latent Group ◽  
Biochemical Indices ◽  
And Oxidative Stress

Abstract The haemato-biochemical indices and oxidative stress markers in horses naturally infected with Trypanosoma evansi were evaluated by analyzing the level of these parameters between T. evansi infected (microscopically positive patent group and PCR positive latent group) and infection free horses. To compare the hemato-biochemical indices and oxidative stress indicators, horses were divided into three categories based on diagnostic test employed and positive results obtained. These included Romanowsky stained slide positive group (Group I; n = 6), PCR positive group (group II; n = 28) and negative control group (group III, n = 30), revealing parasitologically positive patent, molecular positive latent and disease free status of horses. A significant reductions in total erythrocytes count (TEC, P = 0.01), haemoglobin (Hb, P = 0.01) and packed cell volume (PCV, P = 0.04) was noticed both in group I and group II while significant neutrophilia and lymphocytopenia was observed in group I when compared to negative control group. Substantial increase in creatinine (CRTN, P = 0.032) and gamma glutamyl transferase (GGT, P = 0.012) in group I while significant decrease in glucose (GLU, P = 0.04) and iron (Fe, P = 0.01) were noticed in both group I and group II in comparison to group III. A significant difference in lipid peroxides (LPO, P = 0.01) with highest level in patent group I (15.33 ± 0.53) followed by PCR positive latent group (14.09 ± 1.66) indicates higher lipid peroxidation in erythrocytes and oxidative stress in decreasing order when compared with infection free control horses (9.83 ± 0.97). Catalase (CAT, P = 0.01) was significantly lower in parasitological (0.82 ± 0.14) and molecular positive cases (1.27 ± 0.35) in comparison to control group (3.43 ± 0.96). The levels of superoxide dismutase (SOD, P = 0.01), reduced glutathione (GSH, P = 0.01) and ferric reducing antioxidant power (FRAP, P = 0.01) were significantly lower in parasito-molecular positive cases as compared to infection free control horses. An inverse correlation of RBC count with LPO and GSH and a direct correlation with catalase, SOD and FRAP was revealed. Overall, the observed substantial decreases in the oxidative parameters like catalase CAT, SOD, GSH and FRAP activities with remarkably elevated levels of LPO indicate high exposure of erythrocytes to oxidative damage in T.evansi infected horses.

scholarly journals PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus lam) TERHADAP KADAR GLUKOSA DARAH DAN MALONDIALDEHID SERUM MENCIT YANG DIINDUKSI ALOKSAN

2015 ◽  
Vol 34 (1) ◽  
pp. 79
Author(s):  
Husnil Kadri ◽  
Ezy Julianx Jarit ◽  
Erlina Rustam
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Reduce Blood Glucose ◽  
Fruit Oil

AbstrakTujuan penelitian ini adalah untuk menentukan efek pemberian minyak buah merah (pandanus conoideus lam) terhadap kadar glukosa darah dan malondialdehid (MDA) serum mencit yang diinduksi dengan aloksan. Induksi aloksan ini menyebabkan hiperglikemia yang dapat mencetuskan stres oksidatif. Minyak buah merah yang mengandung banyak antioksidan dapat mengendalikan stres oksidatif yang akhirnya dapat menurunkan kadar glukosa darah dan MDA serum.Penelitian eksperimental ini menggunakan 15 ekor mencit jantan yang diseleksi secara random. Hewan coba dibagi menjadi 3 kelompok masing-masing terdiri 5 ekor mencit, yaitu; kelompok kontrol negatif yang hanya diberikan makan dan minum, kelompok kontrol positif yang diinduksi aloksan, dan kelompok perlakuan (diberi minyak buah merah oral) yang diinduksi aloksan. Setelah perlakuan hari ke-14, darah mencit diambil untuk diperiksa kadar glukosa darah dan MDA serum.Hasil penelitian menunjukkan rerata kadar glukosa darah kelompok kontrol negatif adalah 141,20 ± 13,10 mg/dl. Rerata kadar glukosa darah kelompok kontrol positif adalah 266,00 ± 111,10 mg/dl. Rerata kadar glukosa darah kelompok perlakuan adalah 194,80 ± 27,00 mg/dl. Rerata kadar MDA serum kelompok kontrol negatif adalah 0,91 ± 0,10 nmol/ml. Rerata kadar MDA serum kelompok kontrol positif adalah 1,49 ± 0,34 nmol/ml. Rerata kadar MDA kelompok perlakuan adalah 0,97 ± 0,14 nmol/ml. Perbedaan bermakna kadar glukosa darah hanya antara kelompok kontrol negatif dengan kelompok perlakuan (p<0.05). Analisis statistik kadar MDA serum menunjukkan terdapat perbedaan bermakna antara kelompok kontrol positif dengan kelompok perlakuan (p< 0,05).Kesimpulan dari penelitian ini ialah minyak buah merah tidak mampu menurunkan kadar glukosa darah pada kelompok perlakuan, tetapi minyak buah merah mampu menurunkan kadar MDA serum pada kelompok perlakuan.Kata kunci: minyak buah merah, glukosa darah, MDA serum, mencit, aloksanARTIKEL PENELITIAN80AbstractThe objective of this studi was to determine the effect of red fruit oil (pandanus conoideus lam) on blood glucose and serum malondialdehyde in alloxan induced diabetic mice. Hyperglycemia in this mice could lead to oxidative stress. Red fruit oil contains high antioxidants that have capability to control oxidative stress, so blood glucose and serum MDA is reduced.This experimental study had been carried out to 15 rats (randomized group design) which divided into three group of five rats each, i.e. negative control, positive control (alloxan induced), and the group was treated by alloxan induced which followed by red fruit oil orally for 14 days. The result was analyzed by using one way Anova with confidence interval 95%.The result show that blood glucose in negative control was 141.20 ± 13.10 mg/dl. Blood glucose in positive control was 266.00 ± 111.10 mg/dl. Blood glucose in the group was treated by alloxan induced which followed by red fruit orally is 194.80 ± 27.00 mg/dl. The MDA level in negative control group is 0.91 ± 0.10 nmol/ml, positive control group is 1.49 ± 0.34 nmol/ml, and the group was treated by alloxan induced which followed by red fruit orally is 0.97 ± 0.14 nmol/ml. Blood glucose was significantly different between negative control and treated group (p<0.05). Serum MDA was significantly different between positive control group and the group was treated by alloxan induced which followed by red fruit orally (p<0.05).The conclusion is red fruit oil cannot reduce blood glucose level in the group was treated by alloxan induced which followed by red fruit orally, but it has capability to quench serum MDA level in this group.Key word: red fruit oil – blood glucose – serum MDA – mice – alloxan

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