scholarly journals PENGARUH PEMBERIAN MINYAK BUAH MERAH (Pandanus conoideus lam) TERHADAP KADAR GLUKOSA DARAH DAN MALONDIALDEHID SERUM MENCIT YANG DIINDUKSI ALOKSAN

2015 ◽  
Vol 34 (1) ◽  
pp. 79
Author(s):  
Husnil Kadri ◽  
Ezy Julianx Jarit ◽  
Erlina Rustam
Keyword(s):  
Oxidative Stress ◽  
Blood Glucose ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Reduce Blood Glucose ◽  
Fruit Oil

AbstrakTujuan penelitian ini adalah untuk menentukan efek pemberian minyak buah merah (pandanus conoideus lam) terhadap kadar glukosa darah dan malondialdehid (MDA) serum mencit yang diinduksi dengan aloksan. Induksi aloksan ini menyebabkan hiperglikemia yang dapat mencetuskan stres oksidatif. Minyak buah merah yang mengandung banyak antioksidan dapat mengendalikan stres oksidatif yang akhirnya dapat menurunkan kadar glukosa darah dan MDA serum.Penelitian eksperimental ini menggunakan 15 ekor mencit jantan yang diseleksi secara random. Hewan coba dibagi menjadi 3 kelompok masing-masing terdiri 5 ekor mencit, yaitu; kelompok kontrol negatif yang hanya diberikan makan dan minum, kelompok kontrol positif yang diinduksi aloksan, dan kelompok perlakuan (diberi minyak buah merah oral) yang diinduksi aloksan. Setelah perlakuan hari ke-14, darah mencit diambil untuk diperiksa kadar glukosa darah dan MDA serum.Hasil penelitian menunjukkan rerata kadar glukosa darah kelompok kontrol negatif adalah 141,20 ± 13,10 mg/dl. Rerata kadar glukosa darah kelompok kontrol positif adalah 266,00 ± 111,10 mg/dl. Rerata kadar glukosa darah kelompok perlakuan adalah 194,80 ± 27,00 mg/dl. Rerata kadar MDA serum kelompok kontrol negatif adalah 0,91 ± 0,10 nmol/ml. Rerata kadar MDA serum kelompok kontrol positif adalah 1,49 ± 0,34 nmol/ml. Rerata kadar MDA kelompok perlakuan adalah 0,97 ± 0,14 nmol/ml. Perbedaan bermakna kadar glukosa darah hanya antara kelompok kontrol negatif dengan kelompok perlakuan (p<0.05). Analisis statistik kadar MDA serum menunjukkan terdapat perbedaan bermakna antara kelompok kontrol positif dengan kelompok perlakuan (p< 0,05).Kesimpulan dari penelitian ini ialah minyak buah merah tidak mampu menurunkan kadar glukosa darah pada kelompok perlakuan, tetapi minyak buah merah mampu menurunkan kadar MDA serum pada kelompok perlakuan.Kata kunci: minyak buah merah, glukosa darah, MDA serum, mencit, aloksanARTIKEL PENELITIAN80AbstractThe objective of this studi was to determine the effect of red fruit oil (pandanus conoideus lam) on blood glucose and serum malondialdehyde in alloxan induced diabetic mice. Hyperglycemia in this mice could lead to oxidative stress. Red fruit oil contains high antioxidants that have capability to control oxidative stress, so blood glucose and serum MDA is reduced.This experimental study had been carried out to 15 rats (randomized group design) which divided into three group of five rats each, i.e. negative control, positive control (alloxan induced), and the group was treated by alloxan induced which followed by red fruit oil orally for 14 days. The result was analyzed by using one way Anova with confidence interval 95%.The result show that blood glucose in negative control was 141.20 ± 13.10 mg/dl. Blood glucose in positive control was 266.00 ± 111.10 mg/dl. Blood glucose in the group was treated by alloxan induced which followed by red fruit orally is 194.80 ± 27.00 mg/dl. The MDA level in negative control group is 0.91 ± 0.10 nmol/ml, positive control group is 1.49 ± 0.34 nmol/ml, and the group was treated by alloxan induced which followed by red fruit orally is 0.97 ± 0.14 nmol/ml. Blood glucose was significantly different between negative control and treated group (p<0.05). Serum MDA was significantly different between positive control group and the group was treated by alloxan induced which followed by red fruit orally (p<0.05).The conclusion is red fruit oil cannot reduce blood glucose level in the group was treated by alloxan induced which followed by red fruit orally, but it has capability to quench serum MDA level in this group.Key word: red fruit oil – blood glucose – serum MDA – mice – alloxan

scholarly journals Gambaran Glukosa Darah Setelah Latihan Fisik pada Tikus Wistar Diabetes Melitus yang Diinduksi Aloksan

2015 ◽  
Vol 4 (1) ◽  
Author(s):  
Ahmad Syukri Harahap ◽  
Rahmatina B. Herman ◽  
Eti Yerizel
Keyword(s):  
Blood Glucose ◽  
Physical Exercise ◽  
Fasting Blood Glucose ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Diabetes Melitus

AbstrakDiabetes Melitus telah dikategorikan sebagai penyakit global yang prevalensinya terus meningkat dan penyebab utama morbiditas dan mortalitas. Latihan fisik merupakan salah satu tatalaksana untuk mengontrol glukosa darah secara nonfarmakologis. Tujuan dari penelitian ini adalah untuk mengetahui gambaran kadar glukosa darah pada tikus Wistar diabetes melitus yang diinduksi aloksan setelah pemberian latihan fisik. Penelitian eksperimental ini menggunakan 18 ekor tikus Wistar dengan berat badan 150-200 gram yang dibagi menjadi tiga kelompok, yaitu 6 ekor kelompok kontrol negatif (K-), 6 ekor kelompok kontrol positif (K+) dengan induksi aloksan tanpa pemberian latihan fisik dan 6 ekor kelompok perlakuan (P) dengan induksi aloksan dan diberi latihan fisik selama 4 minggu. Pengukuran kadar glukosa darah dilakukan dengan glucometer merek Accu-Check. Hasil penelitian menunjukkan peningkatan rata-rata kadar glukosa darah puasa secara bermakna pada kelompok kontrol positif (K+) dan perlakuan (P) setelah periode induksi yaitu, pada kelompok kontrol negatif 84,83±6,88 mg/dl, kelompok kontrol positif 220,80±12,29 mg/dl, dan kelompok perlakuan 248,50±94,55 mg/dl (p<0,05). Setelah periode latihan fisik, terdapat penurunan rata-rata glukosa darah puasa secara bermakna pada kelompok kontrol positif (K+) dan Perlakuan (P), namun penurunan pada kelompok perlakuan (P) lebih besar secara bermakna dibandingkan dengan kelompok kontrol positif (K+), yaitu rata-rata glukosa darah puasa kelompok kontrol positif 192,00±12,00 mg/dl dan kelompok perlakuan 163,00±20,26 mg/dl (p<0,05). Kesimpulan hasil penelitian ini adalah terdapat penurunan kadar glukosa darah puasa setelah latihan fisik.Kata kunci: latihan fisik, glukosa darah, diabetes melitusAbstractDiabetes Melitus has been categorized as a global disease which have increasing prevalence and the mine cause of morbidity and mortality. Phyisical exercise is one of the nonpharmacological treatment to control blood glucose. The objective of this research was to determine the blood gucose levels of aloksan induced’s wistar rat after physical exercise.The research used 18 wistar rats 150-200 gram weight, divided into three groups, as six negative control group (K-), six positive control group inducing aloksan without physical exercise (K+) and six treated group inducing aloksan with physical exercise for four weeks (P). Blood glucose was measured by Accu Check’s glucometer.The result showed an increase in fasting blood glucose level significantly on positive control group (K-) and treated group (P) after induced period, which was 84,83±6,88 mg/dl on negative control group, 220,80±12,29 mg/dl on positive control group, and 248,50±94,55 mg/dl on treated group (p<0,05). After physical exercise period, there was a decrease in fasting blood significantly on K+ and P, but in P group have more greater than K+ group, 192,00±12,00 mg/dl on positive control group (K+) and 163,00±20,26 mg/dl on treated group (P).Conclusion of this research is a decrease in fasting blood glucose levels after phyisical exercise.Keywords: physical exercise, blood glucose, diabetes melitus

scholarly journals Protective Effect of Potential Probiotic Strains from Fermented Ethiopian Food against Salmonella Typhimurium DT104 in Mice

2020 ◽  
Vol 2020 ◽  
pp. 1-8 ◽  
Author(s):  
Guesh Mulaw ◽  
Diriba Muleta ◽  
Anteneh Tesfaye ◽  
Tesfaye Sisay
Keyword(s):  
Survival Rate ◽  
Growth Inhibition ◽  
Negative Control Group ◽  
Treated Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Mixed Potential ◽  
Probiotic Strains

Salmonella is one of the most harmful pathogens responsible for foodborne outbreaks, illnesses and deaths. The aim of this study was to evaluate the effect of potentially probiotic strains against Salmonella Typhimurium DT104 in mice. The compatibility test among the selected potential probiotic strains (Lactobacillus plantarum K132, Lactobacillus paracasei K114 and Lactococcus lactis E124) using the cross-streaking method showed the absence of antagonism. The anti-Salmonella activities of coculture of the isolated potential probiotics in the form of mixed or single culture showed a remarkable anti-Salmonella activity with 96.50 to 100% growth inhibition. The combination of strains, which showed the highest growth inhibition rates against Salmonella Typhimurium DT104, was used to test their effect on the colonization of mice by Salmonella Typhimurium DT104. White albino male mice were pretreated with the mixed potential probiotics for 7 days and infected with Salmonella Typhimurium DT104 for 1 day. A total of 3 treatments were applied, during which the negative control group was treated with phosphate-buffered saline (PBS); a positive control group (typ) was challenged with Salmonella Typhimurium DT104 alone. The treated group (pro-typ) was pretreated with mixed potential probiotic culture and then infected with Salmonella Typhimurium DT104. The survival rate of mice and counts of Salmonella in feces were recorded. The survival rate of mice on day 21 after the oral challenge with Salmonella Typhimurium DT104 was significantly p<0.05 higher in the experimental pro-typ group (100% survival) compared with the positive control group (20% survival). The counts (colony-forming unit per ml) of Salmonella in feces were significantly lower p<0.05 for the pro-typ group compared to the typ group. The combination of potential probiotic strains was able to protect mice against Salmonella Typhimurium DT104 infection that demonstrates their potential to be used as probiotic cultures for the production of functional fermented products.

Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

2018 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Verawaty Verawaty ◽  
Dhea Claudia Novel
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Male Mice ◽  
Glucose Levels ◽  
The Third ◽  
Blood Glucose Levels

<p>Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.</p><p><em>Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.</em></p>

scholarly journals Resistance to Fracture of Dental Roots Obturated with Different Materials

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Berkan Celikten ◽  
Ceren Feriha Uzuntas ◽  
Kamran Gulsahi
Keyword(s):  
Fracture Resistance ◽  
Testing Machine ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Root Canal Filling ◽  
Filling Materials ◽  
Group 2

The aim of this study was to compare the vertical fracture resistance of roots obturated with different root canal filling materials and sealers. Crowns of 55 extracted mandibular premolar teeth were removed to provide root lengths of 13 mm. Five roots were saved as negative control group (canals unprepared and unfilled). Fifty root canals were instrumented and then five roots were saved as positive control group (canals prepared but unfilled). The remaining 45 roots were randomly divided into three experimental groups (n=15root/group) and obturated with the following procedures: in group 1, glass ionomer-based sealer and cone (ActiV GP obturation system); in group 2, bioceramic sealer and cone (EndoSequence BC obturation system); and in group 3, roots were filled with bioceramic sealer and cone (Smartpaste bio obturation system). All specimens were tested in a universal testing machine for measuring fracture resistance. For each root, the force at the time of fracture was recorded in Newtons. The statistical analysis was performed by using Kruskal-Wallis and post hoc test. There were no significant differences between the three experimental groups. The fracture values of three experimental and negative control groups were significantly higher than the positive control group. Within the limitations of this study, all materials increased the fracture resistance of instrumented roots.

scholarly journals Biosafety devices to control the spread of potentially contaminated dispersion particles. New associated strategies for health environments

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0255533
Author(s):  
Victor Angelo Martins Montalli ◽  
Patrícia Rejane de Freitas ◽  
Milenna de Figueiredo Torres ◽  
Oscar de Figueiredo Torres Junior ◽  
Dienne Hellen Moutinho De Vilhena ◽  
...  
Keyword(s):  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
High Rate ◽  
Positive Control Group ◽  
Control Group ◽  
Healthy Environment ◽  
The Mean ◽  
Petri Dishes ◽  
Uv C

Dental procedures produce a large amount of spatter and aerosols that create concern for the transmission of airborne diseases, such as Covid-19. This study established a methodology with the objective of evaluating new associated strategies to reduce the risk of cross-transmission in a health environment by simulating spread of potentially contaminated dispersion particles (PCDP) in the environment. This crossover study, was conducted in a school clinic environment (4 clinics containing 12 dental chairs each). As a positive control group (without barriers), 12 professionals activated at the same time the turbine of dental drill, for one minute, with a bacterial solution (Lactobacillus casei Shirota, 1.5x108 CFU/mL), which had been added in the cooling reservoir of the dental equipment. In the experimental groups, the professionals made use of; a) an individual biosafety barrier in dentistry (IBBD) which consists of a metal support covered by a disposable PVC film barrier; b) a Mobile Unit of Disinfection by Ultraviolet-C, consisting of 8 UV lamps-C of 95W, of 304μW/cm2 of irradiance each, connected for 15 minutes (UV-C) and; c) the association between the two methods (IBBD + UV-C). In each clinic, 56 Petri dishes containing MRS agar were positioned on the lamps, benches and on the floor. In addition, plates were placed prior to each test (negative control group) and plates were also placed in the corridor that connects the four clinics. In the groups without barrier and IBBD + UV-C the passive air microorganisms in Petri dishes was also evaluated at times of 30, 60, 90 and 120 minutes after the end of the dental’s drill activation. The mean (standard deviation) of CFU of L. casei Shirota for the positive control group was 3905 (1521), while in the experimental groups the mean using the IBBD was 940 (466) CFU, establishing a reduction on average, of 75% (p<0.0001). For the UV-C group, the mean was 260 (309) CFU and the association of the use of IBBD + UV-C promoted an overall average count of 152 (257) CFU, establishing a reduction on average of 93% and 96%, respectively (p<0.0001). Considering these results and the study model used, the individual biosafety barrier associated with UV-C technology showed to be efficient strategies to reduce the dispersion of bioaerosols generated in an environment with high rate of PCDP generation and may be an alternative for the improvement of biosafety in different healthy environment.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

The Effect of Chemically Modified Tetracycline-3 on the Progression of Dental Caries in Rats

2018 ◽  
Vol 52 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Jun Xu ◽  
Congcong Miao ◽  
Zhenchuan Tian ◽  
Jinlu Li ◽  
Chunmei Zhang ◽  
...  
Keyword(s):  
Dental Caries ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Caries Prevalence ◽  
Chemically Modified ◽  
Dentin Caries ◽  
Chemically Modified Tetracycline

Objective: Matrix metalloproteinases (MMPs) exist in human saliva and dentin and play an important role in the degradation of organic matrix in teeth. Chemically modified tetracycline-3 (CMT-3) is an inhibitor of MMPs. CMT-3 has been used experimentally to treat caries since 1999, but no distinction between dental caries prevalence and dentin caries prevalence has been described. Methods: A total of 65 Sprague-Dawley rats were randomly divided into three groups. The positive control group (25 rats) was inoculated with Streptococcus mutans (ATCC700610) and fed the cariogenic feed of improved Keyes Diet 2000. The CMT-3 group (25 rats) was also inoculated with S. mutans and fed the cariogenic feed of improved Keyes Diet 2000; the surfaces of rats’ molars were daily treated with 0.02% CMT-3. The negative control group (15 rats) was only fed the standard rodent chow. At the end of the 10th week, the dental caries prevalence and dentin caries prevalence of each group were calculated, and the regions of caries were assessed. Results: No caries was found in the negative control group. The dental caries prevalence of the CMT-3 and the positive control group was 75.0 and 83.3%, respectively (p > 0.05, Table 2). The dentin caries prevalence of the CMT-3 and the positive control group was 33.3 and 70.8%, respectively (p < 0.05, Table 2). The Keyes scoring of dentin caries in the CMT-3 group was significantly lower than that in the positive control group (p < 0.05, Table 3). Conclusions: CMT-3 had no effect on the prevalence of dental caries, but could lower the prevalence and slow down the progression of dentin caries.

Protective Effect of Curcumin on Carbapenem-Resistant Escherichia coli–Induced Lung Injury in Rats

2016 ◽  
Vol 101 (7-8) ◽  
pp. 304-312 ◽  
Author(s):  
Cagla Bali ◽  
Nejat Altintas ◽  
Ozlem Ozmete ◽  
Ibrahim Gelincik ◽  
Hakan Yabanoglu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Lung Injury ◽  
Intraperitoneal Injection ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
E Coli ◽  
Carbapenem Resistant

Curcumin has remarkable anti-inflammatory and antioxidant properties. The aim of this study was to investigate the protective effects of curcumin on a rat model of carbapenem-resistant Escherichia coli–induced acute lung injury (ALI). Thirty-two rats were randomly allocated to 4 groups to induce an ALI: negative control group (rats not infected with E coli with no antibiotic treatment), positive control group (rats infected with E coli with no antibiotic treatment), imipenem group (rats infected with E coli that received intraperitoneal injection of imipenem), and the imipenem+curcumin group (rats infected with E coli that received intraperitoneal injection of imipenem and were fed on curcumin).The rats were killed, and lung tissues samples were harvested for biochemical analyses and histopathologic examination. Total antioxidant status (TAS), total oxidant status (TOS), tumor necrosis factor α (TNFα), and interleukin-6 (IL6) were measured. TOS increased in the positive control group (P &lt; 0.001) and decreased in the imipenem and imipenem+curcumin groups (P &lt; 0.001 and P &lt; 0.001, respectively). TAS decreased in the positive control group (P = 0.005). Imipenem treatment did not increase TAS, but the imipenem+curcumin group increased TAS (P = 0.014). TNFα and IL6 increased in the positive control group compared with the negative control group (P &lt; 0.001 and P = 0.010, respectively). Imipenem decreased TNFα (P &lt; 0.001), but did not decrease IL6 (P = 0.418). Imipenem+curcumin decreased TNFα (P &lt; 0.001); this decrease was more pronounced compared with the imipenem group (P = 0.008). IL6 decreased in the curcumin group compared with the positive control group (P = 0.011). Curcumin combined with imipenem can be an alternative therapeutic agent to overcome the resistance of E coli strains.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

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