scholarly journals The concentration of androgen receptor and protein kinase A in male chicken following the administration of a combination of the epididymis and testicular extracts

2020 ◽  
Vol 13 (8) ◽  
pp. 1594-1598
Author(s):  
Muslim Akmal ◽  
Gholib Gholib ◽  
Mustafa Kamal Nasution ◽  
Sri Wahyuni ◽  
Rinidar Rinidar ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Protein Kinase A ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Commercial Chicken ◽  
Male Chicken ◽  
Completely Randomized Design ◽  
Kinase A

Background and Aim: Testis (T) and epididymis (E) are waste from the abattoir that is rarely used. In fact, both organs contain important chemicals needed for spermatogenesis (e.g., hormones, proteins, and other molecules). Therefore, administration of a combination of testis and epididymis (CTE) extracts may activate androgen receptors (AR) and protein kinase A (PKA) molecules that play a prominent role in spermatogenesis. We, therefore, aimed at investigating the influence of the CTE extracts on the concentration of AR and PKA in male chicken. Materials and Methods: This study used a completely randomized design with four treatment groups (K0, K1, K2, and K3) and five replications per group. K0 is a control group that received 1 mL normal saline, whereas K1, K2, and K3 are the test groups that received 1, 2, and 3 mL of CET extracts, respectively. Twenty male chickens (strain: broiler Mb 89), 3 weeks of age, weighing 500-700 g were used. We administered the injections in a 13-day period and on the 14th day; we collected and processed blood samples as serum to measure the AR and PKA concentrations using commercial chicken AR and PKA enzyme-linked immunosorbent assay kits, respectively. We performed analyses by analysis of variance using SPSS 20.0. Results: The AR concentrations in K1, K2, and K3 groups increased by 4.26%, 10.97%, and 28.04%, respectively, compared to the K0 (control group). However, this increase was not significantly different between the groups (p>0.05). Moreover, the PKA concentrations increased by 2.97%, 2.60%, and 4.08% in K1, K2, and K3 groups, respectively, compared to the control group. However, this increase was not significantly different between the groups as well (p>0.05). Conclusion: The CTE extracts tended to increase the AR and PKA concentrations even though it is not significant. Therefore, it needs further study when using the CTE extracts for spermatogenesis in male chicken.

scholarly journals THE CAPABILITY OF SEDATIVE EFFECT FROM CELERY (Apium graveolens L.) FRACTION TO MALE MICE

2018 ◽  
Vol 4 (2) ◽  
Author(s):  
Shinta Kusuma ◽  
Arum Setiawan ◽  
Salni Salni
Keyword(s):  
Negative Control Group ◽  
Negative Control ◽  
Apium Graveolens ◽  
Control Group ◽  
Water Fraction ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Traction Test ◽  
Effect Test

Celery fraction research (Apium graveolens L.) was carried out to determine the ability of the sedation effect of celery fraction compared to celery extracts which have been known to have the ability to effect the previous sedation. This study aims to find out which fraction has the best sedation effect. This study was an experimental study with a Completely Randomized Design (CRD) consisting of 5 treatments and 5 replications. Test animals divided into 5 treatment groups namely negative control group (CMC Na 1%), celery extract group 200mg /kg and 3 treatment groups n-hexane fraction, ethyl acetate, and methanol water fraction with a dose of 200 mg /kg . The sedation effect test was carried out using the Traction Test and Fireplace Testmethods. Quantitative data observed were the length of time the mice fell and the length of time the mice went out of the heated tube/glass. The results of the analysis showed that the celery fraction had a better sedation effect than the extract, and the methanol water fraction 200 mg/kg was the most effective fraction in causing sedation effects.

scholarly journals Blood profile and gut microbial load of broilers fed siam weed (Chromolaena odorata) leaf meal in their diets

Agricultura ◽  
2017 ◽  
Vol 14 (1-2) ◽  
pp. 17-24 ◽  
Author(s):  
Peter-Damian Chukwunomso Jiwuba ◽  
Ifeanyi Princewill Ogbuewu ◽  
Elisha Dauda ◽  
Caritas Chibuike Azubuike
Keyword(s):  
Immune Status ◽  
Microbial Load ◽  
Control Group ◽  
Pathogenic Microorganisms ◽  
Chromolaena Odorata ◽  
Blood Profile ◽  
Treatment Groups ◽  
Leaf Meal ◽  
Randomized Design ◽  
Completely Randomized Design

AbstractThe responses of 96 day old broilers fed Chromolaena odorata leaf meal (COLM) on blood profile and gut microbial load were studied for 49 days. The birds were randomly assigned to four treatment groups and replicated three times in a completely randomized design. The birds were fed four experimental diets formulated at 0%, 2%, 4% and 6% for the starter phase and 0%, 4%, 8% and 12% of COLM at the finisher phase for T1, T2, T3 and T4 respectively. All the haematological parameters were similar (P>0.05) across the treatment groups except MCH and WBC which were significantly (P< 0.05) higher and better in the treatment groups than the control group. The results on serum chemistry showed significant (P<0.05) difference for total protein, albumin, creatinine and cholesterol across the treatment groups. Cholesterol was significantly (P<0.05) reduced with increasing levels of COLM. Results showed that coliforms and E.coli were consistently higher (P<0.05) among the T1 birds than those fed T2, T3 and T4. It was concluded that, COLM enhanced adequate haematocrit and immune status, hypoglycaemic ability, suppresses the growth of gut pathogenic microorganisms and enhanced the growth of beneficial microorganism in broilers.

scholarly journals Pengaruh Laserpunktur dan Penambahan Probiotik dalam Laju Pertumbuhan Sapi Bali (Bos sondaicus) Jantan

2021 ◽  
Vol 9 (2) ◽  
pp. 79
Author(s):  
Febriyono Anggela Prakoso ◽  
Raden Tatang Santanu Adikara ◽  
Kusnoto Kusnoto ◽  
Sri Hidanah ◽  
Yeni Dhamayanti ◽  
...  
Keyword(s):  
Growth Rate ◽  
Weight Gain ◽  
Research Methods ◽  
Control Group ◽  
Treatment Groups ◽  
Gain Rate ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Bali Cattle

The purpose of this study was to determine the effect of laserpuncture shoot towards the growth rate of male Bali cattle. This reasearch was done in July until August 2018 in Cendono Village, Pasuruan. This research uses 18 samples with completely randomized design as a research methods. This research used 18 male Bali cattle aged 2 – 2.5 year. This research divided by 3 groups are the control group (P0, which is without laserpuncture shooting) and two treatment groups (P1, which is laserpuncture shooting with 0.5 joule of doses and P2, which is laserpuncture shooting with 0.5 joule of doses and bioplus probiotic). Laserpuncture shooting already done for 6 weeks with 6 days of interval. The result is weight of cuttle is increasing and it is known from digital weight scales. The data is analyzed by using ANOVA test and followed by Duncan. The result is laserpuncture shooting at the growth point can be increasing the weight of male Bali cattle. Stastical comparison amon P0, P1 and P2 showed there were significant difference on growth rate (p<0.05). The result showed that induction of laserpuncture and bioplus probiotic has effect for growth gain rate of male Bali cattle. The optimal rate of weight gain occured in the second week with laserpuncture induction at dose of 0.5 joule and the six week with laserpuncture induction at dose of 0.5 joule and bioplus probiotic.

scholarly journals Effect of feed grade acidifiers on total tract mineral retention, intestinal pH and phytate utilization by broiler finisher chickens

2020 ◽  
Vol 44 (1) ◽  
pp. 282-289
Author(s):  
C. U. Idachaba ◽  
I. Abdullahi ◽  
D. A. Kurtong
Keyword(s):  
Broiler Chickens ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Control Treatment ◽  
Control Group ◽  
Available Phosphorus ◽  
Treatment Groups ◽  
Randomized Design ◽  
Mineral Utilization ◽  
Completely Randomized Design

Mineral chelators such as organic acids have been reported to compete favourably with phytate by forming soluble complexes with feed minerals. Effect of feed grade acidifiers on total tract mineral retention and intestinal pH of broiler finisher chickens was investigated. A total of 300-broiler finisher chickens were assigned to four treatment groups. Each treatment was replicated thrice with twenty chickens per replicate in a completely randomized design. The control treatment (T1 ) was fed the control diet adequate in available phosphorus at 0.5% while other treatments (T2 -T4 ) were fed sub-optimal available phosphorus diets at 0.4%. Three blends of acidifiers; Fysal®, Orgacid® and Acidomix® were supplemented in the diets of chickens in treatments 2, 3 and 4 at 0.1% (1kg per ton/feed). Chickens fed diet supplemented  with Fysal® showed the best (P<0.05) results in total tract mineral retention 56.5+/-5.75 compared to the control and other treatments. The poor mineral retention i.e. 35 and 46% for calcium and phosphorus respectively by chickens fed the control diet was attributed to the insoluble phytate-mineral complex that limits mineral bioavailability. Intestinal segments of chickens fed acidified diets showed significant reduction (P<0.05) in pH in the range of 5.26-7.16 across duodenum to ileum compared to the control group 6.83-8.76. It is concluded that feed acidification at 0.1% improves mineral utilization in broiler chickens as it competes favourably with phytate in the lumen of the gut leading to better nutrient utilization.

scholarly journals Effect of Leaves Infuse (Foeniculum vulgare Mill.) Intake on Reproductive Organs Morphometry of Female Rats (Rattus Sp.) After Parturition

2017 ◽  
Vol 1 ◽  
pp. 27-30
Author(s):  
Najda Rifqiyati ◽  
Jumailatus Sholihah ◽  
Laelatul Soimah
Keyword(s):  
Reproductive Organs ◽  
Female Rats ◽  
Control Group ◽  
Foeniculum Vulgare ◽  
Treatment Groups ◽  
Randomized Design ◽  
Length Width ◽  
Completely Randomized Design ◽  
Layer Chromatography ◽  
Female Reproductive Organs

Fennel (Foeniculum vulgare Mill.) traditionally used by people as antifertility agent for female. Fennel leaves (Foeniculum vulgare Mill.) contain phytoestrogen that has role as antiestrogen or synthesized become antiestrogen. Coumarin is the kind phytoestrogen that can be antiestrogen to female reproductive organs such as ovary and utery. The aim of this research is to find out the influence of fennel leaves infuse (Foeniculum vulgare Mill.) on morphometric of ovary and utery in female rats (Rattus sp.) administered after parturition, to find out the effective dose  affecting the morphometric of ovary and utery and to find out the total coumarin consisted in fennel leaves (Foeniculum vulgare Mill.). This research was carried out by using CRD (Completely Randomized Design) consisted of 2 groups, those are control group (aquades) and treatment groups (doses of fennel leaves infuse 20 g/300 ml, 40 g/300 ml and 60 g/300 ml). The treatment was administered in the morning and afternoon for 15 days. After 15 days of treatment, the rats were sacrificed and dissected to measure the morphometry of reproductive organs (ovary and utery). The data obtained was analyzed by Analysis of Varian (ANOVA) and continued by LSD (Least Significance Different) test with significance of 5%. The measurement of coumarin level was performed by TLC (Thin Layer Chromatography) densitometry method. The result of this research showed that the administration of infused fennel leaves (Foeniculum vulgare Mill.) at dose of 20 g/300 ml, 40 g/300 ml and 60 g/300 ml significantly decreased ovarian weigh in female rats (Rattus sp.) after parturition (p<0,05), but not decreased the  length, width, thick of ovary, length and diameter of utery (p>0,05). No difference was observed between doses 20 g/300 ml, 40 g/300 ml and 60 g/300 ml, and it showed that the dose 20 g/300 ml of fennel leaves infuse was able to be used to decrease the weigth of ovary. Coumarin level observed in leaves is 0,09%, and it is higher than the coumarin content in fruit/seed.

scholarly journals The concentration of testosterone, pituitary adenylate cyclase-activating polypeptide, and protamine 1 in the serum of male chicken following administration of epididymis and testicular extracts and their combination

2019 ◽  
Vol 12 (7) ◽  
pp. 1101-1107 ◽  
Author(s):  
Gholib Gholib ◽  
Rinidar Rinidar ◽  
Fitriani Fitriani ◽  
T. Zahrial Helmi ◽  
Sugito Sugito ◽  
...  
Keyword(s):  
Adenylate Cyclase ◽  
Reproductive Organs ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Treatment Groups ◽  
Significant Difference ◽  
Pituitary Adenylate Cyclase ◽  
Male Chicken ◽  
And Control ◽  
Protamine 1

Background and Aim: Testis and epididymis are male reproductive organs that play an important role in spermatogenesis. These two organs are rich in the content of hormones and other molecules needed in the process of spermatogenesis which affect the quality of the spermatozoa. The objective of this study was to examine the effect of the administration of epididymis and testicular extracts and their combination on testosterone, pituitary adenylate cyclase-activating polypeptide (PACAP), and protamine 1 (PRM1) concentrations in the serum of male chicken. Materials and Methods: Twenty male chickens (broiler strain Cp707), aged 3 weeks and weighing 800-1000 g, were randomly divided into four different groups including a control group (T0) = injected with 1 ml normal saline and treatment groups: T1 = injected with 1 ml epididymis extract, T2 = injected with 1 ml testicular extract, and T3 = injected with a combination of 1 ml epididymis + 1 ml testicular extract. The experiment was conducted for 13 days and at the end of the study (day 14), the chickens were sacrificed to obtain the serum. Furthermore, the concentrations of testosterone, PACAP, and PRM1 were then measured by using an enzyme-linked immunosorbent assay technique. Results: The concentrations of PACAP and PRM1 did not show a significant difference between treatment groups (T1, T2, and T3) and control group (T0) (p>0.05). However, the concentration of testosterone showed a significantly higher difference in a group injected with a combination of 1 ml epididymis and 1 ml testicular extracts (T3) compared to the control group (T0) (p<0.05). Conclusion: The administration of epididymis and testicular extracts and their combination did not affect the increase of PACAP and PRM1 concentration. However, a combination of these extracts significantly affects the increase of testosterone concentration in the serum of male chicken.

Prediction of Survival in Patients with Non-Hodgkin’s Lymphoma: Role of Tumor Necrosis Factor Ligand-Receptor and Extracellular Protein Kinase A.

Blood ◽  
2005 ◽  
Vol 106 (11) ◽  
pp. 4737-4737
Author(s):  
Chul Won Choi ◽  
Soo-Young Yoon ◽  
Hwa Jung Sung ◽  
In Keun Choi ◽  
Seok Jin Kim ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Tumor Necrosis Factor ◽  
Protein Kinase A ◽  
Patient Group ◽  
Tumor Necrosis ◽  
Extracellular Protein ◽  
Control Group ◽  
Necrosis Factor ◽  
Kinase A

Abstract Backgrounds: The International Prognostic Index (IPI) is the most commonly used survival parameter for patients with Non-Hodgkin’s lymphoma (NHL). To investigate an another factor to predict survival, we studied the role of tumor necrosis factor receptor with molecular weight of 75 kd (p75-R-TNF) and extracellular protein kinase A (ECPKA). TNF has a central role in inflammatory processes, and its receptor is constitutively found in the circulation and is elevated in a variety diseases. The cAMP-dependent protein kinase (PKA) is critically involved in the regulation of metabolism, cell growth and differentiation, and gene expression. PKA is a predominantly intracellular enzyme, but it has been shown that cancer cells of various cell types excrete PKA into the conditioned medium. This extracellular form i.e., ECPKA is known to be upregulated in the serum of cancer patients as compared with normal serum. The aim of this prospective study was to evaluate p75-R-TNF and ECPKA as feasible prognostic factors for patients with NHL. Methods: From October 2003 to May 2005, chemotherapy-naive patients with NHL who were planned to receive CEOP-B or R-CHOP chemotherapy at Guro Hospital, Korea University were enrolled. Blood sampling for p75-R-TNF and ECPKA was done before chemotherapy initiation and was stored at −70°C until the assay. The level of p75-R-TNF was measured using ELISA kit. ECPKA was measured by RIA method. A total of 20 serum samples from normal people were used as control. Results: A total of 45 patients were enrolled. The male to female ratio was 22:23, and the median age was 58 years old (range: 29–87). Indolent histologic type and aggressive type were 4 and 41 patients, respectively. Twenty-two patients were limited stage (stage I, II) and 23 patients were advanced stage (stage III, IV). The level (mean±SD) of p75-R-TNF was 1066.6±1174.9 pg/ml for patient group and that of control group was 678.2±312.4 pg/ml. ECPKA activity of patient group was 87.6±21.1 mU/ml as compared with 40.6±21.5 mU/ml in control group. During the median follow-up period of 8.5 months, 12 patients died and 33 patients were alive. As a result of univariate analysis, serum albumin (p=0.005), hemoglobin (p=0.054), the IPI score [0–2 vs 3–5] (p=0.006), occurrence of febrile neutropenia (p=0.01), and level of p75-R-TNF (p=0.003) were significantly associated with survival. By logistic regression testing, p75-R-TNF level was identified as an independent predictive factor for survival (p=0.037). Median survival of patients with elevated p75-R-TNF (cutoff: 678.2 pg/ml) was 10.5 months. For patients with p75-R-TNF < 678.2 pg/ml, median value was not reached yet. Conclusions: This study indicates that in addition to the IPI, high baseline levels of p75-R-TNF can predict the survival of patients with NHL. The levels of ECPKA were elevated in patient group than those of normal controls, but its level could not predict the prognosis.

scholarly journals Effect of Nano herbal Haramounting (Rhodomyrtus tomentosa) in Histology of Heart Mus musculus

2019 ◽  
Vol 1 (2) ◽  
pp. 101-106
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Research Use ◽  
Treatment Groups ◽  
High Energy Milling ◽  
Randomized Design ◽  
Significant Difference ◽  
Completely Randomized Design ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the effect of histology of heart Mus musculus  after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the  Completely Randomized Design (CRD). Treatment consists of 6 groups, namely:  Control: CMC 0.5%; T1:100mg/20gBW; T2: 141,42mg/20gBW; T3:200mg/20gBW; T4: 282,82mg/20gBW;  T5:400mg/20gBW. The results of histology hearts that there were significant difference (P<0,05) between  control group and treatment groups but there was not significantly different in weight of hearts (P>0,05).

scholarly journals PENGARUH AIR REBUSAN DAUN HARENDONG BULU (Clidemia hirta) TERHADAP KADAR GULA DARAH MENCIT (Mus musculus)

2020 ◽  
Vol 3 (1) ◽  
pp. 7-12
Author(s):  
Okta Malia ◽  
Dian Samitra ◽  
Eka Lokaria
Keyword(s):  
Blood Sugar ◽  
Mus Musculus ◽  
Design Research ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Sugar Levels ◽  
Boiled Water ◽  
One Way Anova

This study aims to determine the effect of the water boiled leaves of Clidemia hirta on blood sugar levels in Swiss Webster mice (Mus musculus). This research using a completely randomized design research design (CRD) with 5 treatment groups namely; Aquades control group (P0), 3 g sucrose treatment (P1), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.13 ml/KgBB (P2), 3 g sucrose treatment + boiled bristle leaves boiled water at a dose of 0.26 ml/KgBB (P3), 3 g treatment of sucrose + boiled bristle leaves boiled water at a dose of 0.39 ml/KgBB (P4). Before administration of the extract, mice in the treatment groups P1, P2, P3 & P4 were induced using 3 g of sucrose for 5 days. Then after 5 days for groups P2, P3, and P4 followed by giving boiled water leaves for 7 days. The analysis result of One Way Anova Test shows that Fcount > Ftable (18.86 > 2.51). This shows the boiled water affects the blood sugar levels of male Swiss Webster mice. The concentration of extract at P4 (0.39 ml/KgBB) was more effective in reducing blood sugar levels.

scholarly journals Description of Kidney Histology of Mus Musculus After Giving Nano Hebal Rhodomyrtus tomentosa (Haramounting)

2019 ◽  
Vol 1 (1) ◽  
pp. 26-33
Author(s):  
Syafruddin Ilyas
Keyword(s):  
Mus Musculus ◽  
High Energy ◽  
Control Group ◽  
Treatment Groups ◽  
Randomized Design ◽  
Significant Difference ◽  
Proximal Tubular ◽  
Completely Randomized Design ◽  
Paraffin Method ◽  
Rhodomyrtus Tomentosa

The objective of this study was to determine the description of kidney histology of Mus musculus after giving Nano Herbal Rhodomyrtus tomentosa (Haramonting). R.tomentosa (Haramonting) is made on a nanoscale using High Energy Milling (HEM). This research use the Completely Randomized Design (CRD), which consists of six groups of CMC 0.5% and Nano- R.tomentosa (Haramonting) at dosage of (100; 141,42; 200; 282,82; 400)mg/20g body weight. The kidneys were made into preparations by using the paraffin method and Hematoxylin Erlich-Eosin staining (HE). The results of histological kidneys showed that there is a significant difference (P<0,05) between control group and treatment groups with damaged cells and increased proximal tubular narrowing in kidneys, but there was not significantly different in weight of kidneys (P>0,05).

Sign in / Sign up

Export Citation Format

Share Document