scholarly journals Endophytes isolated from passion fruit plants: molecular identification, chemical characterization and antibacterial activity of secondary metabolites

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Edinardo Fagner Ferreira Matias ◽  
Erivânia Ferreira Alves ◽  
Beatriz Sousa Santos ◽  
Celestina Elba Sobral de Souza ◽  
João Victor de Alencar Ferreira ◽  
...  

Knowledge of medicinal plants is often the only therapeutic resource of many communities and ethnic groups. “Erva-baleeira”,Cordia verbenaceaDC., is one of the species of plants currently exploited for the purpose of producing a phytotherapeutic product extracted from its leaves. In Brazil, its major distribution is in the region of the Atlantic Forest and similar vegetation. The crude extract is utilized in popular cultures in the form of hydroalcoholic, decoctions and infusions, mainly as antimicrobial, antiinflammatory and analgesic agents. The aim of the present study was to establish a chemical and comparative profile of the experimental antibacterial activity and resistance modifying activity with ethnopharmacological reports. Phytochemical prospecting and HPLC analysis of the extract and fractions were in agreement with the literature with regard to the presence of secondary metabolites (tannins and flavonoids). The extract and fraction tested did not show clinically relevant antibacterial activity, but a synergistic effect was observed when combined with antibiotic, potentiating the antibacterial effect of aminoglycosides. We conclude that tests of antibacterial activity and modulating the resistance presented in this work results confirm the ethnobotanical and ethnopharmacological information, serving as a parameter in the search for new alternatives for the treatment of diseases.


2018 ◽  
Vol 13 (1) ◽  
pp. 11-21 ◽  
Author(s):  
Huibin Zhao ◽  
Chuner Cai ◽  
Xiaoyu Liu ◽  
Binghua Jiao ◽  
Bo Chen ◽  
...  

AbstractPolar microbial derived antibiotics have potential as alternatives to traditional antibiotics in treating fish against pathogenic bacteria. In this paper, 23 strains of polar fungi were fermented to detect bacteriostatic products on three aquatic pathogenic bacteria, subsequently the active fungus was identified. It was indicated that secondary metabolites of 23 strains weredistinct; of these, the extract of strain B-7 (belonging toBjerkanderaaccording to molecular identification) demonstrated a strong antibacterial activity toStreptococcus agalactiae,Vibrio anguillarumandAeromonas hydrophilaATCC7966 by Kirby-Bauerpaper strip method. During one fermentation cycle, the pH curve of the fermentation liquor became lowest (4.0) on the 4thday and rose back to 7.6 finally after 5 days, The residual sugar curve was decreased before stablising on the 6thday. It is presumed that a large amount of alkaline secondary metabolites might have been produced during fermentation. This study focuses on antagonism between aquatic pathogenic bacteria and fermentation metabolites from Antarctic fungi for the first time, which may provide data on research of antibiotics against aquatic pathogenic bacteria.


2021 ◽  
Vol 22 (10) ◽  
Author(s):  
Syarifah Syarifah ◽  
Elfita Elfita ◽  
HARY WIDJAJANTI ◽  
ARUM SETIAWAN ◽  
ALFIA R. KURNIAWATI

Abstract. Syarifah, Elfita, Widjajanti H, Setiawan A, Kurniawati AR. 2021. Diversity of endophytic fungi from the root bark of Syzygium zeylanicum, and the antibacterial activity of fungal extracts, and secondary metabolite. Biodiversitas 22: 4572-4582. The decoction of the root bark of Syzygium zeylanicum has been used as traditional medicine, such as for treating pathogenic bacterial infections. Endophytic fungi that live in medicinal plant tissues have a high species diversity and biological activities correlate with their host. Therefore, this study aimed to explore the diversity of endophytic fungi from the root bark of S. zeylanicum and to determine the antibacterial activity of endophytic fungi and their secondary metabolites. In this study, we isolate and identify the endophytic fungi from the root bark of S. zeylanicum, continued by screening their antibacterial activity against two Gram-negative bacteria (Escherichia coli InaCCB5 and Salmonella thypi ATCC1048 and two Gram-positive bacteria (Staphylococcus aureus InaCCB4 and Bacillus subtilis InaCCB1204) by the Kirby-Bauer method. The fungal extract with the highest antibacterial activity proceeded with the isolation and determination of the structure of their bioactive compounds. The isolates were morphologically identified. Isolates that showed strong antibacterial activity were identified by molecular identification. Isolation of bioactive compounds was carried out by chromatographic techniques and the determination of the structure of pure chemical compounds was performed by the spectroscopic analysis. In total, there were 8 isolates of endophytic fungi were obtained from the root bark of S. zeylanicum, namely SZR1 – SZR8. SZR2 isolate has the highest antibacterial activity. Molecular identification through phylogenetic analysis showed that SZR2 isolate had high similarity with Penicillium brefeldianum. Isolation of bioactive compounds from SZR2 produced compound 1 in the form of light yellow crystals which showed strong antibacterial activity against S. typhi, E. coli, and B. subtilis with MIC values of 64 g/mL. Compound 1 was identified as p-hydroxybenzaldehyde, which was also obtained in its host. In conclusion, the endophytic fungus Penicillium brefeldianum produces similar secondary metabolites and antibacterial activity as its host plant.


Molecules ◽  
2018 ◽  
Vol 23 (1) ◽  
pp. 146 ◽  
Author(s):  
Vladimir Morocho ◽  
Andrea Valle ◽  
Jessica García ◽  
Gianluca Gilardoni ◽  
Luis Cartuche ◽  
...  

2015 ◽  
Vol 8 (3) ◽  
Author(s):  
Amornrat Aung-aud-chariya ◽  
Phuwadol Bangrak ◽  
Saisamorn Lumyong ◽  
Worrapong Phupong ◽  
Nelly Siababa Aggangan ◽  
...  

Nanomaterials ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 96
Author(s):  
John Jackson ◽  
Helen Burt ◽  
Dirk Lange ◽  
In Whang ◽  
Robin Evans ◽  
...  

The prompt treatment of burn wounds is essential but can be challenging in remote parts of Africa, where burns from open fires are a constant hazard for children and suitable medical care may be far away. Consequently, there is an unmet need for an economical burn wound dressing with a sustained antimicrobial activity that might be manufactured locally at low cost. This study describes and characterizes the novel preparation of a silver nitrate-loaded/poly(vinyl alcohol) (PVA) film. Using controlled heating cycles, films may be crosslinked with in situ silver nanoparticle production using only a low heat oven and little technical expertise. Our research demonstrated that heat-curing of PVA/silver nitrate films converted the silver to nanoparticles. These films swelled in water to form a robust, wound-compatible hydrogel which exhibited controlled release of the antibacterial silver nanoparticles. An optimal formulation was obtained using 5% (w/w) silver nitrate in PVA membrane films that had been heated at 140 °C for 90 min. Physical and chemical characterization of such films was complemented by in vitro studies that confirmed the effective antibacterial activity of the released silver nanoparticles against both gram positive and negative bacteria. Overall, these findings provide economical and simple methods to manufacture stable, hydrogel forming wound dressings that release antibiotic silver over prolonged periods suitable for emergency use in remote locations.


2020 ◽  
Vol 8 (2) ◽  
pp. 61
Author(s):  
Tessalonica Dajoh ◽  
Robert A Bara ◽  
Esther Angkouw ◽  
Medy Ompi ◽  
Rosita A Lintang ◽  
...  

Phyllidiella nigra is an organism that is suspected to have secondary metabolites because their ability to develop its self defense system by camouflage and using chemical compounds derived from their nature diet as deterrent against their predators. The purpose of this study was to isolate symbiotic bacterial derived from P. nigra, extracted and followed by, the antibacterial assays against Escherichia coli and Bacillus megaterium as well as the anti-UV assay. The results showed that the five isolates tested had an antibacterial activity with the highest average inhibition zone against E. coli DSM 498 bacteria, isolate 1 (14.67 mm), isolate 5 (14 mm), and against B. Megaterium DSM 32T bacteria, isolate 3 (13.33 mm). The three isolates which had the highest inhibition zone and P. nigra extract were tested for anti-UV assay using a UV-Vis Spectrophotometer. The results obtained isolate 3 has absorption of UV-A with the UV absorbtion maximum at λ 340 nm and P. nigra extract has absorption on UV-B radiation with UV absorption maximum at λ 290 nm. Key words: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV Phyllidiella nigra merupakan organisme yang diduga memiliki metabolit sekunder karena mampu mengembangkan sistem pertahanan dirinya dengan cara kamuflase dan menggunakan senyawa kimia sebagai racun yang didapat dari makanannya. Tujuan dari penelitian ini yaitu mendapatkan isolat bakteri yang bersimbiosis dengan P. nigra, mendapatkan ekstrak dari baktri simbion, dan menguji antibakteri dan anti-UV ekstrak etil aseta bakteri simbion dengan metode difusi agar terhadap bakteri Escherichia coli dan Bacillus megaterium. Hasil penelitian didapatkan kelima isolat yang diuji memiliki aktivitas antibakteri dengan rerata zona hambat tertinggi terhadap bakteri E. coli DSM 498 yaitu isolat 1 (14,67 mm), isolat 5 (14 mm), dan terhadap baktri B. megaterium DSM 32T yaitu isolat 3 (13,33 mm). Ketiga isolat yang memiliki zona hambat tertinggi dan ekstrak P. nigra diujikan anti-UV menggunakan alat UV-Vis Spektrofotometer. Hasil yang didapat isolat 3 memiliki serapan terhadap radiasi sinar UV-A dengan puncak tertinggi pada λ 340 nm dan ekstrak P. nigra memiliki serapan terhadap radiasi sinar UV-B dengan puncak tertinggi berada pada λ 290 nm. Kata kunci: Nudibranchia, Bacteria, Anti-bacteial, Anti-UV


2020 ◽  
Vol 11 (3) ◽  
pp. 4569-4577
Author(s):  
Sincy Varghese ◽  
Kanakasabapathi Devaki ◽  
Poornima Kannappan ◽  
Sri Rashmy Madathil

Quantitative and qualitative analysis of different components and antioxidant and activities of the extract of flower (CPF) were analyzed in . identification of of flower was also identified by GC-MS analysis. assess the biochemical features of CPF. solvent extraction of CPF was performed using solvents in increasing order of polarity (petroleum ether, chloroform, ethyl acetate, ethanol, and water) and solvent with maximum profile was standardized for further analysis. Quantitative analysis of selected secondary metabolites like tannin, , alkaloids, and of the flower extract was done by UV . In antioxidant assays and in efficacy of the flower extract were analyzed by respective in assays. identification of in CPF was identified by using GC-MS analysis revealed secondary metabolites in the extract, and further analysis of the extract was performed. Quantitative estimation revealed an accountable amount of secondary metabolites like (47.66mg/g acid equivalent), (24mg/g equivalent), (41mg/g equivalent), and alkaloids (1.79mg/g of extracted plant material). analysis (GC-MS) also confirmed convincing compounds in the extract. From in antioxidant and assay, the IC50 value of the extract of CPF was measured and compared with standard, and from the results, it was evident that the extract had significant in antioxidant and activity. the above results, it can be confirmed thatCPF has got significant and therapeutically active ingredients, as evident in analysis. This is further supported by considerable antioxidant and properties observed in respective assays.


Irriga ◽  
2018 ◽  
Vol 23 (4) ◽  
pp. 727-740 ◽  
Author(s):  
Lourival Ferreira Cavalcante ◽  
Clodoaldo Júnior Oliveira Santos ◽  
José Simplício de Holanda ◽  
Antonio João de Lima Neto ◽  
Antônio Gustavo de Luna Souto ◽  
...  

PRODUÇÃO DE MARACUJAZEIRO AMARELO NO SOLO COM CALCÁRIO E POTÁSSIO SOB IRRIGAÇÃO COM ÁGUA SALINA     LOURIVAL FERREIRA CAVALCANTE1; CLODOALDO JÚNIOR OLIVEIRA SANTOS1; JOSÉ SIMPLÍCIO DE HOLANDA2; ANTONIO JOÃO DE LIMA NETO3; ANTÔNIO GUSTAVO DE LUNA SOUTO4 E TONY ANDRESON GUEDES DANTAS5     1 Universidade Federal da Paraíba, Departamento de Solos e Engenharia Rural, Rodovia BR 079 - Km 12, 58.397-000, Areia, PB, Brasil. E-mail: [email protected]; [email protected]  2 Empresa de Pesquisa Agropecuária do Rio Grande do Norte, Av. Eliza Branco Pereira dos Santos, s/nº, Parque das Nações, 59.158-160, Parnamirim, RN, Brasil. E-mail: [email protected] 3 Universidade Federal do Ceará, Departamento de Fitotecnia, Campus do Pici, Av. Mister Hull, 2977, Bloco 805, 60.356-001, Fortaleza, CE, Brasil. E-mail: [email protected] 4 Universidade Federal da Paraíba, Departamento de Fitotecnia e Ciências Ambientais, Rodovia BR 079 - Km 12, 58.397-000, Areia, PB, Brasil. E-mail: [email protected] 5 Instituto Federal de Educação, Ciência e Tecnologia do Ceará, Rodovia CE-187, s/n, Aeroporto, 62.320-000, Tianguá, CE, Brasil. E-mail: [email protected]     1 RESUMO   O experimento foi conduzido no município de Coronel Ezequiel, Rio Grande do Norte, para avaliar os efeitos do calcário calcítico e doses de K2O, na forma de cloreto de potássio, nos componentes de produção do maracujazeiro amarelo e no aumento da salinidade do solo provocado pela irrigação com água salina de 3,6 dS m-1 durante o período da aridez e na lixiviação dos sais do ambiente radicular das plantas promovida pelas águas do período chuvoso. Os tratamentos foram dispostos em delineamento em blocos casualizados usando arranjo fatorial 3 × 2, referente as doses de calcário de 1,4; 2,5 e 3,6 t ha-1, 80 e 160 kg ha-1 de K2O na forma de cloreto de potássio. Pelos resultados, a irrigação com água salina (3,6 dS m-1), em comparação com dados da literatura de plantas irrigadas com água de boa qualidade, não comprometeu a capacidade produtiva do maracujazeiro amarelo. Dentre os tratamentos, a combinação de 80 kg ha-1 de K2O com 3,6 t ha-1 de calcário calcítico proporcionou os maiores valores de massa média dos frutos, produção por planta e produtividade da cultura. Apesar da alta salinidade da água de irrigação elevar o caráter salino do solo no ambiente radicular das plantas durante o período da estiagem, as águas do período chuvoso e as condições físicas do solo proporcionam a lixiviação dos sais e possibilitam o uso de água com restrições salinas na agricultura.   Palavras-chave: Passiflora edulis, calagem, lixiviação de sais     CAVALCANTE, L. F.; SANTOS, C. J. O.; HOLANDA, J. S.; LIMA NETO, A. J.; SOUTO, A. G. L.; DANTAS, T. A. G. YELLOW PASSION FRUIT PLANTS PRODUCTION ON SOIL WITH LIME AND POTASSIUM UNDER IRRIGATION WITH SALINE WATER       2 ABSTRACT   The experiment was carried out in Coronel Ezequiel county, Rio Grande do Norte, Brazil, in order to evaluate the effects of limestone and chloride potassium in production components of yellow passion fruit plants and soil salinity, caused by irrigation with saline water of 3.6 dS m-1 during the dry season, and salt leaching of the soil promoted by waters of the rainy season. Treatments were arranged in randomized blocks using factorial design of 3 × 2, referring to three levels of limestone,  1.4, 2.5 and 3.6 t ha-1 and two potassium levels,  80 and 160 kg ha-1 in potassium chloride form. According to present results, irrigation with saline water (3.6 dS m-1), in comparison with data from the literature about plants irrigated with non-saline water, did no compromise the productive capacity of yellow passion fruit. Among the treatments, the combination of 80 kg ha-1 of K2O with 3.6 t ha-1 of limestone provided the fruits production with more mean mass, yield per plant and crop yield. Although the high salinity of the irrigation water increases the soil saline character on root environment of the plants during the dry season, the rainy season waters and the soil physical conditions provide the salt leaching and allow the use of water with saline restrictions in agriculture.   Keywords: Passiflora edulis, liming, salt lixiviation


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