scholarly journals Soybean Seedlings Enriched with Iron and Magnesium - Impact on Germination, Growth and Antioxidant Properties

2018 ◽  
Vol 25 (4) ◽  
pp. 631-641
Author(s):  
Jagna Chmielowska-Bąk ◽  
Inga Zinicovscaia ◽  
Marina Frontasyeva ◽  
Aleksandra Milczarek ◽  
Sabrina Micheli ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Germination Rate ◽  
Antioxidant Properties ◽  
Soybean Seeds ◽  
Intellectual Performance ◽  
Mg Deficiency ◽  
Soybean Seedlings ◽  
Fortified Food ◽  
The World ◽  
Fe Uptake

Abstract Iron (Fe) and magnesium (Mg) deficiency in human diets is a widespread problem observed in various regions of the world. Insufficient Fe uptake results in the development of iron dependent anaemia and depressed physical and intellectual performance. In turn Mg deficiency is associated with alterations in neuromuscular and cardiovascular systems. An emerging alternative to traditional supplementation of these elements in the form of pills, liquids or effervescent tablets, is introduction of fortified food products. In present study we show that preincubation of soybean seeds in Fe and Mg solutions leads to elevated content of these elements in the seedlings. Importantly the pretreatment did not affect germination rate, seedlings growth or, with an exception of Fe supplementation at highest concentration, antioxidant capacity. The obtained results indicate that preincubation of seeds in Fe and Mg solutions may be a promising method of obtaining enriched soybean sprouts.

scholarly journals Exogenous melatonin reduces the inhibitory effect of osmotic stress on antioxidant properties and cell ultrastructure at germination stage of soybean

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0243537
Author(s):  
Mingcong Zhang ◽  
Songyu He ◽  
Bin Qin ◽  
Xijun Jin ◽  
Mengxue Wang ◽  
...  
Keyword(s):  
Water Deficit ◽  
Lipid Membrane ◽  
Germination Rate ◽  
Antioxidant Properties ◽  
Cell Ultrastructure ◽  
Water Deficit Stress ◽  
Soybean Seeds ◽  
Peg 6000 ◽  
Exogenous Melatonin ◽  
Germination Stage

Understanding the relationship between exogenous melatonin and water deficit stress is crucial for alleviating the effects of water deficit stress at germination stage of soybean (Glycine max (L.) Merrill) in agriculture. This study investigated the effects of exogenous melatonin on soybean antioxidant properties and cell ultrastructure under water deficit stress induced by polyethylene glycol (PEG) 6000. The drought-sensitive soybean variety Suinong 26 was used as the material to study the effects of different concentrations of melatonin (0, 300, 500 μmol·L-1) soaking soybean seeds under drought stress (PEG-6000: 3% and 6%). The results showed that the germination rate (GR), germination potential (GP), germination index (GI) and radicle shape of soybean were affected negatively to different degrees under PEG stress. Moreover, stress induced by different PEG concentrations overproduced the content of reactive oxygen species (H2O2, O2·−) in cells, leading to increased lipid membrane peroxidation as electrolyte leakage (EL) and malondialdehyde (MDA) content, which resulted in impaired cell integrity. However, after seeds soaking with melatonin, the lipid peroxidation of the cell membrane was reduced, and the activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) further increased to minimize the excessive generation of ROS. Similar results were obtained for soluble protein and proline, that may help in regulating the osmotic pressure and maintain cellular integrity. With the interaction of these enzymes, compared with 300 μmol·L-1 melatonin, 500 μmol·L-1 melatonin could more effective to remove the ROS and reduce cell peroxidation. Overall, 500 μmol·L-1 melatonin performed better than 300 μmol·L-1. In conclusion, the seed soaking with melatonin promoted the germination of soybean seeds under water stress.

HPLC Screening of Bioactives Compounds and Antioxidant Capacity of Different Hypericum Species

2018 ◽  
Vol 69 (2) ◽  
pp. 305-309 ◽  
Author(s):  
Daniela Gitea ◽  
Simona Vicas ◽  
Manuel Alexandru Gitea ◽  
Sebastian Nemeth ◽  
Delia Mirela Tit ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Methanol Extract ◽  
Antioxidant Properties ◽  
Polyphenolic Compounds ◽  
The Other ◽  
The North ◽  
Western Area ◽  
North Western ◽  
Hypericum Species ◽  
Biological Actions

Our study compares the content in polyphenolic compounds and hypericin, in four species of Hypericum - H. perforatum L., H. maculatum Cr., H. hirsutum L., H. tetrapterum Fr. (syn. Hypericumacutum Mnch.) harvested from spontaneous flora in the north-western area of Transylvania, Romania. These species represent an important source of such compounds with different biological actions. After making the extracts, they were subjected to HPLC-SM analysis. The presence of rutoside in the largest amount (462.82 mg %) in the H. perforatum extract was observed, this containing most of the flavonoid heterosides. For the species H. maculatum, the presence in a much higher amount of the hyperoside (976.36 mg %) is characteristic compared to the other species. Quercetol is the best represented of the flavonoid aglycons, its concentration being the highest in H. hirsutum (659.66 mg %). The hypericin content ranges from 0.2171 g % in the H. tetrapterum extract, to 0.0314 g % in the methanol extract of H. maculatum.The highest antioxidant properties measured by FRAP method were recorded in the case of H. perforatum and H. maculatum.

scholarly journals Comparative Study on Seed Characteristics, Antioxidant Activity, and Total Phenolic and Flavonoid Contents in Accessions of Sorghum bicolor (L.) Moench

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3964
Author(s):  
Bimal-Kumar Ghimire ◽  
Ji-Won Seo ◽  
Chang-Yeon Yu ◽  
Seung-Hyun Kim ◽  
Ill-Min Chung
Keyword(s):  
Antioxidant Activity ◽  
Agronomic Traits ◽  
Germination Rate ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Extensive Study ◽  
Total Phenolic ◽  
Phytochemical Content ◽  
Seed Characteristics ◽  
Seed Extracts

Sorghum is a major cereal food worldwide, and is considered a potential source of minerals and bioactive compounds. Its wide adaptive range may cause variations in its agronomic traits, antioxidant properties, and phytochemical content. This extensive study investigated variations in seed characteristics, antioxidant properties, and total phenolic (TPC) and flavonoid contents (TFC) of sorghum collected from different ecological regions of 15 countries. The antioxidant potential of the seed extracts of various sorghum accessions was determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2′-azinobis 3-ethylbenzothiazoline-6-sulfonate (ABTS) radical scavenging assays. Significant variations in TPC were observed among the sorghum accessions. All 78 sorghum accessions used in this study exhibited significant variations in TFC, with the lowest and highest amount observed in accessions C465 and J542, respectively. DPPH scavenging potential of the seed extracts for all the accessions ranged from 11.91 ± 4.83 to 1343.90 ± 81.02 µg mL−1. The ABTS assay results were similar to those of DPPH but showed some differences in the accessions. Pearson’s correlation analysis revealed a wide variation range in the correlation between antioxidant activity and TPC, as well as TFC, among the sorghum accessions. A wide diversity range was also recorded for the seed characteristics (1000-seed weight and seed germination rate). A dendrogram generated from UPGMA clustering, based on seed traits, antioxidant activity, TPC, and TFC was highly dispersed for these accessions. Variations among the accessions may provide useful information regarding the phytoconstituents, antioxidant properties, and phytochemical contents of sorghum and aid in designing breeding programs to obtain sorghum with improved agronomic traits and bioactive properties.

scholarly journals Variation of the Polyphenolic Composition and Antioxidant Capacity of Freshly Prepared Pomegranate Leaf Infusions over One-Day Storage

Antioxidants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1187
Author(s):  
Manyou Yu ◽  
Irene Gouvinhas ◽  
Ana Barros
Keyword(s):  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Punica Granatum ◽  
Added Value ◽  
Plant Materials ◽  
Polyphenolic Composition ◽  
Punica Granatum L ◽  
Intensive Search ◽  
By Products

In recent decades, an intensive search for natural and novel types of antioxidant polyphenolics has been carried out on numerous plant materials. However, the current literature has very little information on their storage stability in the form of freshly prepared infusions. This study aims to characterize the polyphenolic composition and the antioxidant capacity of pomegranate (Punica granatum L.) leaf infusions over one-day storage (analyzed at 0, 2, 4, 6, 8, and 24 h). Spectrophotometric evaluation demonstrated that the infusion presented no significant changes in the content of total phenols (131.40–133.47 mg gallic acid g−1) and ortho-diphenols (239.91–244.25 mg gallic acid g−1). The infusion also maintained high stability (over 98% and 82%, respectively) for flavonoids (53.30–55.84 mg rutin g−1) and condensed tannins (102.15–124.20 mg epicatechin g−1), with stable (>90%) potent antioxidant capacity (1.5–2.2 mmol Trolox g−1) throughout 0–24 h storage. The main decrease was observed during 0–2 h storage of flavonoids, 8–24 h storage of tannins, and 0–4 h storage of antioxidant capacity. Chromatographic analysis further revealed that 7 decreased and 11 increased compounds were found within 0–24 h storage. The good stability of the total polyphenolics and antioxidant properties might be related to the complex conversion and activity compensation among these compounds. The findings suggest that pomegranate leaf infusion could be of great interest in the valorization of high added-value by-products and in the application of green and functional alternatives in the food-pharma and nutraceutical industries.

scholarly journals Formulation Effects in the Antioxidant Activity of Extract from the Leaves of Cymbopogon citratus (DC) Stapf

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4518
Author(s):  
Raquel Sousa ◽  
Artur Figueirinha ◽  
Maria Teresa Batista ◽  
Maria Eugénia Pina
Keyword(s):  
Antioxidant Activity ◽  
Perennial Grass ◽  
Antioxidant Properties ◽  
Dosage Forms ◽  
Oral Dosage ◽  
Dpph Assay ◽  
Cymbopogon Citratus ◽  
The World ◽  
Food And Beverage ◽  
Oral Dosage Forms

Cymbopogon citratus DC (Stapf.) is a perennial grass and it is distributed around the world. It is used as a condiment for food and beverage flavouring in the form of infusions and decoctions of its dried leaves. Our previous studies have shown antioxidant, anti-inflammatory and gastroprotective activities for the infusion and its phenolic fractions. The aim of the present work was to develop oral dosage forms from a Cymbopogon citratus extract to be used as a functional food with antioxidant properties. Initially, an essential oil-free infusion was prepared, lyophilized and characterized by HPLC-PDA. Total phenols were quantified with the Folin–Ciocalteu method and the antioxidant activity was assessed by DPPH assay. Gelatine capsules containing the extract with different excipients, selected after DSC and IR trials, were prepared. A formulation exhibiting better antioxidant behaviour in a gastric environment was attained. These results suggest that the proposed formulation for this extract could be a valuable antioxidant product and, consequently, make an important contribution to “preventing” and minimizing diseases related to oxidative stress conditions.

scholarly journals Isolation and structural elucidation of dimeric epigallocatechin-3-gallate autoxidation products and their antioxidant capacity

2021 ◽  
Author(s):  
Julian Alfke ◽  
Uta Kampermann ◽  
Svetlana Kalinina ◽  
Melanie Esselen
Keyword(s):  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Cd Spectroscopy ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Published Data ◽  
Dietary Polyphenols ◽  
Cellular Effects ◽  
The Impact

AbstractDietary polyphenols like epigallocatechin-3-gallate (EGCG)—which represents the most abundant flavan-3-ol in green tea—are subject of several studies regarding their bioactivity and health-related properties. On many occasions, cell culture or in vitro experiments form the basis of published data. Although the stability of these compounds is observed to be low, many reported effects are directly related to the parent compounds whereas the impact of EGCG degradation and autoxidation products is not yet understood and merely studied. EGCG autoxidation products like its dimers theasinensin A and D, “P2” and oolongtheanin are yet to be characterized in the same extent as their parental polyphenol. However, to investigate the bioactivity of autoxidation products—which would minimize the discrepancy between in vitro and in vivo data—isolation and structure elucidation techniques are urgently needed. In this study, a new protocol to acquire the dimers theasinensin A and D as well as oolongtheanin is depicted, including a variety of spectroscopic and quadrupole time-of-flight high-resolution mass spectrometric (qTOF-HRMS) data to characterize and assign these isolates. Through nuclear magnetic resonance (NMR) spectroscopy, polarimetry, and especially circular dichroism (CD) spectroscopy after enzymatic hydrolysis the complementary atropisomeric stereochemistry of the isolated theasinensins is illuminated and elucidated. Lastly, a direct comparison between the isolated EGCG autoxidation products and the monomer itself is carried out regarding their antioxidant properties featuring Trolox equivalent antioxidant capacity (TEAC) values. These findings help to characterize these products regarding their cellular effects and—which is of special interest in the flavonoid group—their redox properties.

Antioxidant Properties and Polyphenol Screening of the Leaves of Native Hungarian Oak (Quercus) Species

2021 ◽  
Vol 17 ◽  
Author(s):  
Tamás Hofmann ◽  
Eszter Visi-Rajczi ◽  
Levente Albert
Keyword(s):  
Antioxidant Capacity ◽  
State Of The Art ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Flavonoid Glycosides ◽  
Waste Biomass ◽  
Quercus Species ◽  
Tandem Mass Spectrometric ◽  
Liquid Chromatographic ◽  
Phenolic Acid Derivatives

Background: Due to their ecological significance and timber value, Quercus species are especially important in Hungary. Nevertheless, the leaves of these species lack a dedicated utilization field and are considered a waste biomass. Materials and Methods: The present study comprehensively analyses three selected oak species (Q. petraea, Q. pubescens, Q. cerris) native to Hungary to assess their antioxidant capacity (FRAP, ABTS, DPPH) and provide information on their polyphenol pool using state-of-the-art liquid chromatographic/tandem mass spectrometric technique. To the best of our knowledge, no such investigation has yet been conducted for the assigned species. Results: According to the results, the antioxidant capacity of the three species’ leaves are roughly equal. Altogether, 109 compounds have been tentatively identified and described, including phenolic acid derivatives, tannins, flavonoid glycosides, and catechins. Compared to other oak leaf samples and other types of plant tissues, the investigated samples contained a large number (24) of acylated polyphenols. Conclusion: The recent findings on the excellent antioxidant and antibacterial properties of acylated polyphenols suggest that the investigated samples could also be beneficial to human health, requiring further analysis.

scholarly journals Qualities of Native Apple Cultivar Juices Characteristic of Central Europe

2012 ◽  
Vol 40 (1) ◽  
pp. 222 ◽  
Author(s):  
Otakar ROP ◽  
Martin POSOLDA ◽  
Jiri MLCEK ◽  
Vojtech REZNICEK ◽  
Jiri SOCHOR ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Ascorbic Acid ◽  
Hydroxyl Radical ◽  
Antioxidant Capacity ◽  
Antioxidant Properties ◽  
Landscape Management ◽  
Ascorbic Acid Content ◽  
Fruit Trees ◽  
Apple Fruit ◽  
Scavenging Effect

During the last century due to the changes in landscape management, in country people’s lives and with intensive fruit-growing the native cultivars of apple fruit trees have been gradually disappearing. The aim of the study was to compare the juices made of native apple cider cultivars. The cultivars-‘Boikovo’, ‘Jadernicka moravska’, ‘Kardinal zihany’, ‘Panenske ceske’, ‘Parmena zlata zimni’, ‘Strymka’ growing in the locality of the Litencicke Hills in the eastern part of the Czech Republic were selected. For comparison, the fruits of commercial ‘Idared’ cultivar were also included. The chemical composition, antioxidant capacity, phenols, flavonoids and ascorbic acid content and the influence of juices on scavenging activity of nitric oxide and hydroxyl radical were measured. The mutual ratio of examined properties which were found, provide information about qualities and possibilities of use of native cultivars. High antioxidant properties characterize the juice of native apple cultivars. In particular, the ‘Strymka’ cultivar contained 2,637.34 mg of AAE (ascorbic acid equivalent) per litre in case of antioxidant capacity. In the juice of this cultivar the value of 144.05 mg of ascorbic acid per litre was recorded. As regards the ‘Panenske ceske’ cultivar, antioxidant capacity was 2,548.38 mg of AAE l-1 and in relation to ascorbic acid, the value was 145.35 mg l-1. Similarly, high values were observed in both cultivars concerning the scavenging effect of apple juices on hydroxyl radical and nitric oxide (the ‘Strymka’ cultivar 16.38% and 19.26%, the ‘Panenske ceske’ cultivar 16.31% and 18.60%).

scholarly journals Physicochemical and Bioactive Characteristics of Wild Grown Bilberry (Vaccinium myrtillus L.) Genotypes from Northeastern Turkey

2018 ◽  
Vol 46 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Ferit CELIK ◽  
Mehmet Ramazan BOZHUYUK ◽  
Sezai ERCISLI ◽  
Muttalip GUNDOGDU
Keyword(s):  
Vitamin C ◽  
Antioxidant Capacity ◽  
Gallic Acid ◽  
Antioxidant Properties ◽  
Fruit Weight ◽  
Total Phenolic ◽  
Soluble Solid Content ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Antioxidant Power

In present study, fruit weight, fruit firmness, external fruit color, soluble solid content, pH, titratable acidity, vitamin C, total phenolics, total anthocyanins, and antioxidant capacity of eight wild bilberry grown in Coruh valley in northeastern Turkey were determined. The blueberry cv. ‘Bluecrop’ is also included study to make comparison with bilberry genotypes. Antioxidant activity of bilberry and blueberry fruits were comparatively assessed by FRAP (Ferric reducing antioxidant power) assay. Significantly higher content of phenolics and anthocyanins was found in bilberry fruits than in blueberry fruits. However blueberry cv. ‘Bluecrop’ exhibited higher fruit weight and vitamin C content than bilberry fruits. Total phenolic and total anthocyanin content was 327 mg gallic acid equivalent and 142 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruit in cv. ‘Bluecrop’ while it was between 492-563 mg gallic acid equaivalent and 307-342 mg of cyanidin-3-glucoside equivalent in 100 g fresh fruits of  bilberry accessions. Moreover, wild accessions approximately had 2-3 folds higher antioxidant capacity than cv. ‘Bluecrop’. Results suggest the possibility of improving the bioactive and antioxidant properties of bilberry cultivars based food products by using wild ones in cross breeding. It was also concluded that genotypes significantly affect their bioactive content and consequently the possibility of using wild bilberry fruits as a potential source of natural antioxidants in food industry.

scholarly journals Proteomic Analysis of Irradiation with Millimeter Waves on Soybean Growth under Flooding Conditions

2020 ◽  
Vol 21 (2) ◽  
pp. 486 ◽  
Author(s):  
Zhuoheng Zhong ◽  
Takashi Furuya ◽  
Kimitaka Ueno ◽  
Hisateru Yamaguchi ◽  
Keisuke Hitachi ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Proteomic Analysis ◽  
Millimeter Waves ◽  
Sugar Metabolism ◽  
Immunoblot Analysis ◽  
Glutathione Metabolism ◽  
Soybean Seeds ◽  
Soybean Seedlings ◽  
Promotive Effect ◽  
Trehalose Synthesis

Improving soybean growth and tolerance under environmental stress is crucial for sustainable development. Millimeter waves are a radio-frequency band with a wavelength range of 1–10 mm that has dynamic effects on organisms. To investigate the potential effects of millimeter-waves irradiation on soybean seedlings, morphological and proteomic analyses were performed. Millimeter-waves irradiation improved the growth of roots/hypocotyl and the tolerance of soybean to flooding stress. Proteomic analysis indicated that the irradiated soybean seedlings recovered under oxidative stress during growth, whereas proteins related to glycolysis and ascorbate/glutathione metabolism were not affected. Immunoblot analysis confirmed the promotive effect of millimeter waves to glycolysis- and redox-related pathways under flooding conditions. Sugar metabolism was suppressed under flooding in unirradiated soybean seedlings, whereas it was activated in the irradiated ones, especially trehalose synthesis. These results suggest that millimeter-waves irradiation on soybean seeds promotes the recovery of soybean seedlings under oxidative stress, which positively regulates soybean growth through the regulation of glycolysis and redox related pathways.

Sign in / Sign up

Export Citation Format

Share Document