Saccharum officinarum juice alters reproductive functions in male Wistar rats

2020 ◽  
Vol 31 (4) ◽  
Author(s):  
Eunice Ogunwole ◽  
Olufadekemi T. Kunle-Alabi ◽  
Opeyemi O. Akindele ◽  
Yinusa Raji
Keyword(s):  
Wistar Rats ◽  
Dietary Habits ◽  
Sperm Quality ◽  
Sperm Count ◽  
Fasting Blood Glucose ◽  
Male Partner ◽  
Saccharum Officinarum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Water Control ◽  
Male Wistar Rats

AbstractInfertility is a problem across almost all cultures and societies. Problems in the male partner, especially as a result of unhealthy dietary habits, are the commonest single group of course. Many populations, therefore, tend more toward the use of natural dietary substitutes opined to proffer less risk to reproductive functions and more health benefits. Saccharum officinarum juice (SOJ) is a widely consumed, energy-rich, nutritious substance that has many minerals and enzymes. Saccharum officinarum plant was reported to have anti-thrombosis, anti-inflammatory and immune-stimulatory activities. This study evaluated the reproductive effects of S. officinarum juice in male Wistar rats. A sugarcane press juicer was used to extract S. officinarum juice. Twenty male Wistar rats (100–120 g) grouped into four (n = 5) received 1.0 mL/kg/day distilled water (control), and 1.0, 3.2 and 10.0 mL/kg/day of fresh S. officinarum juice once daily for 8 weeks via gavage. Sperm analysis, histology of testes and epididymides were evaluated by microscopy. Enzyme-linked immunosorbent assay (ELISA) was used in assessing the serum levels of luteinizing hormone, follicle-stimulating hormone and testosterone. Data were analyzed using the analysis of variance at a significance of p < 0.05. SOJ increased fasting blood glucose levels in 3.2 and 10.0 mL/kg groups. The 10.0 mL/kg juice caused a significant increase in testosterone level and sperm count, and it also increased the percentage of aberrant sperm and decreased sperm viability. Saccharum officinarum juice impaired the histological integrity of the testes and epididymides. Thus, S. officinarum juice adversely altered the reproductive functions of male Wistar rats by reducing sperm quality and disrupting testicular architecture.

scholarly journals Effect of an Aqueous Extract of Alchornea Cordifolia (Euphorbiacea) Leaves on Sperm Parameters and Reproductive Function of Male Wistar Rats

2021 ◽  
Vol 17 (17) ◽  
Author(s):  
Kone Allassane ◽  
Gnahoue Goueh ◽  
Diabate Daouda ◽  
Kouakou Koffi
Keyword(s):  
Traditional Medicine ◽  
Reproductive System ◽  
Wistar Rats ◽  
Sperm Quality ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Reproductive Function ◽  
Positive Effects ◽  
Male Wistar Rats ◽  
Reproductive Male

Alchornea cordifolia (euphorbiaceae) is a tree whose leaves are used in traditional medicine to cure several health problems. Little information, however, exist on the effects of this herb on the male reproductive system. In order to assess the effect of Alchornea cordifolia on reproductive male parameters and testosterone production, twenty-four male wistar rats divided into four groups of six rats were used. The aqualus extract of Alchornea cordifolia was administered at a dose of 100 mg/kg of bw, 200 mg/kg of bw, and 400 mg/kg of bw per day, orally for 60 days. A significant increase in androgen-dependent organs, testosterone quantity, mobility, and sperm concentration was observed at doses of 200 mg/kg of bw and 400 mg/kg of bw. Only the unwinger sperm count significantly increased at the 100 mg/kg of bw dose. These results revealed that the aqualius extract of the leaves of Alchornea cordifolia has the potential to improve sperm quality and have positive effects on the reproductive system.

scholarly journals The Effect of Soy Milk on Mounting Latency, Mounting Frequency, and Reproductive Development in Male Wistar Rats (Rattus Norvegicus)

2021 ◽  
Vol 9 (B) ◽  
pp. 670-678
Author(s):  
Nurdiana Nurdiana ◽  
Pradnyawati Chania ◽  
Rifzi Nurvitasari ◽  
Azmiatun Nisa ◽  
Styan Wahyu Diana ◽  
...  
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Reproductive Development ◽  
Normal Diet ◽  
Male Rats ◽  
Male Rat ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Soy Milk ◽  
Male Wistar Rats

AIM: This research aims to examine the effects of soy milk on mounting latency (ML), mounting frequency (MF), estrogen levels, androgen-binding protein (ABP) expression, and spermatogenesis in male rats (Rattus norvegicus). METHODS: Twenty-four male wistar rats (Rattus norvegicus) aged 4 weeks were divided into four groups. Control group (given a normal diet), P1; P2; P3 (given the normal diet and soy milk powder at doses of 7.1; 14.2; 21.3 g/KgBW/day, respectively) for 6 weeks. Observation of ML and MF were performed at 9 weeks 5 days of age, and rat surgery was performed at 10 weeks of age. Analysis of estrogen hormone levels was conducted by enzyme-linked immunosorbent assay (ELISA), ABP staining was using immunohistochemistry method, testicular spermatogenesis was observed using histopathological methods, and observation of spermatozoa was performed under the microscope.  RESULTS: The results showed no significant reduction of ML and MF, estrogen levels, and ABP expression (p ≤ 0.256; 0.865; 0.959, respectively) in male rat, but there was a significant decrease in the number, morphology, motility of spermatozoa, and testicular histophatology, (p ≤ 0.000, 0.003, 0.008, 0.000, respectively). CONCLUSION: The administrassion of soy milk in various doses (7.1;14.2;21.3 g/KgBW/day) in male Wistar rats (Rattus norvegicus) had showed significantly difference on histopathological evaluation using Johnson’s scoring system, sperm quantity and quality, while on mounting latency and frequency, estrogen levels, and ABP expressions did not show significantly difference between groups. That describe of isoflavone in soy milk can affect several aspects related to male endocrine and reproductive development.

scholarly journals PENGARUH JAMU DENGAN Tribulus terrestris TERHADAP KUALITAS SPERMA TIKUS WISTAR JANTAN (Rattus norvegicus)

2015 ◽  
Vol 3 (2) ◽  
Author(s):  
Delano Pelealu ◽  
Lydia Tendean ◽  
Benny Wantouw
Keyword(s):  
Experimental Study ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Sperm Morphology ◽  
Sperm Quality ◽  
Control Group ◽  
Tribulus Terrestris ◽  
Normal Morphology ◽  
Normal Sperm ◽  
Male Wistar Rats

Abstract: It is known that Tribulus terrestris can improve sperm quality. One of the Indonesian traditional medicine, jamu, contains this Tribulus terrestris. This study aimed to investigate the effect of jamu containing Tribulus terrestris on sperm quality which consisted of concentration, motility, and morphology of spermatozoa of male wistar rats (Rattus norvegicus). This was an experimental study. Nine male wistar rats were randomly divided into 3 groups of 3 rats each: Group P0 (control), group P1 (given 5 mg of Tribulus terrestris), and group P2 (given 10 mg of Tribulus terrestris). This study was conducted for 52 days. The results showed that the spermatozoa concentration of P1 was 49x106 sperm/ml suspension and of P2 53x106 sperm/ml suspension; both were lower than of P0 (59x106 sperm/ml suspension). The sperm motility category (A+B) of P1 was 33% and of P2 (37%); both were lower than of P0 (45%). The motility category A was not found in P1 and P2. The percentage of spermatozoa with normal morphology of P1 was 45% and the abnormal ones was 55%, meanwhile in P2 there was 49% of normal sperm morphology and 51% of abnormal morphology. Conclusion: Jamu containing Tribulus terrestris had no effect in improvement of the sperm quality.Keywords: jamu, Tribulus terrestris, sperm qualityAbstrak: Tribulus terrestris dikenal sebagai bahan yang dapat memperbaiki kualitas sperma. Salah satu jenis jamu yang diproduksi di Indonesia mengandung Tribulus terrestris Penelitian ini bertujuan untuk mengetahui pengaruh jamu dengan Tribulus terrestris terhadap konsentrasi, motilitas, dan morfologi spermatozoa tikus wistar jantan (Rattus norvegicus). Penelitian ini menggunakan metode eksperimental. Sampel 9 ekor tikus wistar jantan (Rattus norvegicus) dibagi menjadi 3 kelompok yakni, kelompok P0 (kontrol); kelompok P1 (pemberian Tribulus terrestris 5 mg); dan kelompok P2 (pemberian Tribulus terrestris 10 mg). Penelitian dilakukan selama 52 hari. Hasil penelitian menunjukkan bahwa konsentrasi spermatozoa P1 49x106 sperma/ml suspensi dan P2 53x106 sperma/ml suspensi lebih rendah dibandingkan P0 59x106 sperma/ml suspensi. Motilitas spermatozoa kategori A+B pada P1 (33%) dan P2 (37%) lebih rendah dibandingkan pada P0 (45%). Tidak ditemukan motilitas kategori A pada P1 dan P2. Morfologi spermatozoa normal pada P1 sebesar 45% dan abnormal 55%. P2 memiliki morfologi spermatozoa normal sebesar 49% dan abnormal 51%. Simpulan: Pemberian jamu dengan Tribulus terrestris tidak meningkatkan kualitas sperma yang mencakup konsentrasi, motilitas, dan morfologi spermatozoa.Kata kunci: jamu, Tribulus terrestris, kualitas sperma

scholarly journals The supplementation effect of Red Dragon fruit’s skin extract on the fasting blood glucose and lipid profiles in male Wistar rats with diabetes mellitus and dyslipidemia

2020 ◽  
Vol 3 (3) ◽  
pp. 87-92
Author(s):  
Stefani Marietta ◽  
AAG Budhiarta ◽  
I Wayan Weta
Keyword(s):  
Blood Glucose ◽  
Lipid Profile ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Mean Difference ◽  
Control Group ◽  
Skin Extract ◽  
Before And After ◽  
Male Wistar Rats ◽  
Mean Differences

Background: Flavonoids, saponins, tannins, phenols, and vitamin-C contained in the Red Dragon fruit’s skin have a positive impact on glycemic control and lipid oxidation. This study aimed to determine the effect of Red Dragon fruit’s skin extract on reducing the fasting blood glucose (FBG) and improving the lipid profile of Wistar rats with diabetes and dyslipidemia. Methods: A randomized pre-test post-test control group experimental study was done on 22 male Wistar rats, aged 2-3 months that suffered from diabetes and dyslipidemia. Subjects were divided into the control group (given 2cc distilled water + 9 mg metformin) and the treatment group (given 160 mg red dragon fruit’s skin extract + 9 mg metformin) for 14 days. FBG and lipid profile measurements were done before and after the treatment. Data were analyzed using the compare mean test. Results: There was no significant mean difference of GDP between groups before (p=0.414) and after treatment (p=0.125), total cholesterol between groups before (p = 0.572) and after treatment (p=0.361), triglycerides between groups before (p=0.073) and after treatment (p=0.111). There was a significant mean difference of HDL between groups before (p=0.003) and after treatment (p=0.047), LDL between groups before (p=0.006) and after treatment (p=0.043). Although there were significant mean differences in HDL and LDL between groups before and after treatment, the pre-post treatment of HDL and LDL mean differences showed no significant mean difference (p=0.328 and p=0.704 consecutively). Conclusion: Red Dragon fruit’s skin extract treatment did not significantly reduce the mean FBG and lipid profile levels.

scholarly journals Gastro-protective Effects of Honey in Male Wistar Rats

2021 ◽  
pp. 4114-4125
Author(s):  
Owoyele B.V. ◽  
Ayinla M.T. ◽  
Esan A.A. ◽  
Bayo-Olugbami A.
Keyword(s):  
Gastric Acidity ◽  
Wistar Rats ◽  
Control Group ◽  
Therapeutic Effects ◽  
Protective Effects ◽  
Water Control ◽  
Significant Difference ◽  
Male Wistar Rats ◽  
Pre Treatment ◽  
Laboratory Models

Honey is consumed as food and also used in the treatments of ailment. However, honey of various types exhibit varying properties. Their therapeutic effects are determined by whether the honey is multifloral or monofloral, and also on the variety of nectars the honey is derived from. Manuka and Tualang types of honey were observed to possess anti-ulcer effects. However, no such report is available for University of Ilorin honey. This study therefore aimed to determine the gastroprotective effects of University of Ilorin honey in Wistar rats using two laboratory models for inducing ulcer (HCl/Ethanol and Indomethacin). Twenty rats each were assigned to four groups for each model of ulcer. The rats were treated for 12 days with the administration of distilled water (control), cimetidine (100 mg/kg b.w.) and University of Ilorin honey (250 mg/kg b.w. in one group and 1000 mg/kg b.w in another group). The rats were fasted for 24 hours after the last treatment. Then, the ulcerogens were given orally. After four hours, the rats were sacrificed, gastric juice was collected and the stomachs were examined for ulceration. The results revealed that pre-treatment with University of Ilorin honey reduced gastric index from 6.80±0.20 (1000 mg/kg b.w. to 2.40±0.24 (control) P<0.05 and 7.20±0.37 (1000 mg/kg b.w.) to 3.20±0.37 (control) P<0.05 in HCl/Ethanol and indomethacin induced ulcer models respectively. There was no significant difference in ulcer index between the cimetidine administered rats and the honey administered group (1000 mg/kg bw.), but the honey group (250 mg/kg bw.) was less effective than the cimetidine group in the models used. Also, there were no changes in gastric acidity in all the groups compared with the control group. In conclusion, the results showed that University of Ilorin honey has protective effect on the gastrointestinal tract which is not due to alteration of gastric acidity.

scholarly journals ANNONA MURICATA LINN. ETHANOLIC LEAF EXTRACT AMELIORATES REPRODUCTIVE COMPLICATIONS IN STREPTOZOTOCIN-INDUCED DIABETIC WISTAR RATS

2020 ◽  
Vol 18 (1) ◽  
pp. 166-175
Author(s):  
O. E. ADELEYE ◽  
N. A. ABOAJAH ◽  
A. I. ADELEYE ◽  
E. A.O. SOGEBI ◽  
F. M. MSHELBWALA ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Sperm Count ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Male Rats ◽  
Annona Muricata ◽  
Major Health Problem ◽  
Ethanolic Leaf Extract

Diabetes mellitus is an endocrine and metabolic disorder of humans and animals characterized by hyperglycemia and low blood insulin levels or insensitivity of target organs to insulin and it’s a major health problem affecting patient’s quality of life due to its many complications. Infertility is one of the major secondary complications in diabetes. Although numerous drugs have been used for intervention studies on diabetes-induced infertility worldwide, there are currently no treatments for diabetes associated infertility in humans. This study was performed to investigate the effects of Annona muricata ethanolic leaf extract (AMELE) on fertility of male diabetic rats and levels of blood glucose. Twenty male Wistar rats (150-200g) were randomly distributed into 4 groups (n=5) treated thus: CTRL (control), DNT, DT1 and DT2 (diabetic, single intraperitoneal injection, streptozotocin, 60 mg/kg). Group DT1 and DT2 received AMELE orally at 100 mg/kg and 200 mg/kg respectively daily for fourteen days. Data were analysed using ANOVA at α0.05. The animals were sacrificed after 2 weeks via thiopental injection and testicular weights were recorded. Fasting blood glucose was determined using a digital glucometer. Sperm count, motility, viability and morphology were assessed microscopically. Testes were histologically evaluated. The results showed that oral administration of AMELE at 100 mg/kg and 200 mg/kg to diabetic male rats for fourteen days significantly decreased blood glucose level and also ameliorated diabetes-induced decreases in sperm functions in streptozotocin-induced diabetic male rats.    

scholarly journals Analysing the Hypolipidemic Activities of the Tea Extracts of Moringa oleifera, Glycyrrhiza glabra and their Blend at Different Concentrations, Orally Induced on Adult Male Wistar Rats.

2016 ◽  
Vol 3 ◽  
pp. 76-83
Author(s):  
Olanrewaju Roland Akinseye
Keyword(s):  
Wistar Rats ◽  
Moringa Oleifera ◽  
Glycyrrhiza Glabra ◽  
Atherogenic Index ◽  
Simultaneous Increase ◽  
Hypolipidemic Effect ◽  
Aqueous Extracts ◽  
Water Control ◽  
Beneficial Effects ◽  
Male Wistar Rats

Herbal teas ofMoringa oleifera(Moringa) andGlycyrrhiza glabra(Licorice)were known to have many beneficial effects, their possible hypolipidemic effects were investigated in this paper. Albino wistar rats were fed with aqueous extracts ofM. oleifera,G. glabraand their blend teas(10, 30 and 50 mg/kg.BW/ml). The teas were found to lower the serum cholesterol, triglycerides, VLDL, LDL, and atherogenic index at their higher concentration, but were found to slightly increase the HDL as compared to the corresponding normal healthy rats fed with clean water (control). The phytochemicals screening of the teas aqueous extract were also investigated, the chemicals (tannins, saponins, flavonoids, terpenoids and glycosides) which were known to exhibit cholesterol lowering effect by inhibiting its absorption and simultaneous increase its excretion were found to be present in the teas extract. Thus, the study demonstrates and validates thatM.oleifera,G. glabraand their blend possess hypolipidemic effect at higher concentration.

scholarly journals Glycaemic, Lipidaemic and Antioxidant Profiles of Alloxan-Induced Diabetic Wistar Rats Treated with Glibenclamide and Aqueous Extract of Gongronema latifolium (Benth)

2016 ◽  
Vol 8 (4) ◽  
pp. 408-413
Author(s):  
Patrick Emeka ABA
Keyword(s):  
Wistar Rats ◽  
Combined Therapy ◽  
Low Density Lipoprotein ◽  
Fasting Blood Glucose ◽  
Density Lipoprotein ◽  
Study Groups ◽  
Oral Route ◽  
Diabetic Rats ◽  
Glucose Levels ◽  
Male Wistar Rats

The current study investigated the ameliorative effects of combined therapy of glibenclamide and G. latifolium (GL) on several biochemical parameters of alloxaized Wistar rats. Thirty adult male Wistar rats assigned into 5 groups of 6 rats each were used for the study. Groups 2-5 were intraperitoneally injected with 160 mg/kg of alloxan monohydrate and upon establishment of diabetes (Fasting Blood Glucose (FBG) ≥ 126 mg/dl) were treated with 10 ml/kg distilled water (DW), 2 mg/kg glibenclamide, 200 mg/kg GL and 2 mg/kg glibenclamide and 200 mg/kg GL respectively. Rats in group 1 were not made diabetic and served as normal control. All the treatments were realized through daily oral route using gastric tube, for 21 days. Results indicated that the treatment of diabetic rats with a combination of glibenclamide and GL significantly reduced the elevated glucose levels, cholesterol, triacylglycerol, low density lipoprotein and malondialdehyde levels, along with increases in the high density lipoprotein, glutathione values and catalase activities, when compared to diabetic untreated group. It was concluded that the combined therapy of glibenclamide and GL showed superior antihyperglycemic, hypolipidaemic and antioxidant effects compared to either of them used alone.

The effect of Achillea millefolium extract on spermatogenesis of male Wistar rats

2010 ◽  
Vol 30 (4) ◽  
pp. 328-334 ◽  
Author(s):  
Nasrin Takzare ◽  
Mir-Jamal Hosseini ◽  
Seyedeh Hamideh Mortazavi ◽  
Sahar Safaie ◽  
Rayhaneh Moradi
Keyword(s):  
Adult Male ◽  
Seminiferous Tubule ◽  
Wistar Rats ◽  
Sperm Count ◽  
Basal Membrane ◽  
Achillea Millefolium ◽  
Seminiferous Tubules ◽  
Native Plant ◽  
Cell Accumulation ◽  
Male Wistar Rats

Achillea millefolium or yarrow, a native plant in many countries, has been recognized in historical medicine, mainly because of its astringent effects. However, some aspects of the toxicity of yarrow such as possible effects on male reproductive system are not well established. In this investigation, the effects of A. millefolium L. extract on spermatogenesis in adult male wistar rats were studied. Eighty-five male Wistar rats were divided into nine experimental groups (10 in each group except the ninth group). Extract was administered at the dose of 200,400 and 800 mg/kg/day by intraperitoneal (IP) injection or through gavage for 22 days, on every other day. Three groups were determined as sham and control groups. Five rats from each group were killed and the rest of the rats were kept for 40 days later, but with no injection, to assess the reversibility of extract effect on spermatogenesis. The results of the study showed scattered immature cells on basal membrane in seminiferous tubules at the dose of 400 mg/kg/day IP. Moreover, a significant decrease in cell accumulation and vacuolization in seminiferous tubule was seen. At the dose of 800 mg/kg, IP, thickened seminiferous tubules on basal membrane, decrease in cell accumulation in seminiferous tubule, severe disarrangement, degenerative cells and severe decrease in sperm count were seen. At the dose of 800 mg/kg/day, orally, basal membrane was thickened and the disarrangement in cells was demonstrated. As a conclusion, our results suggest that the total extract of A. millefolium L. exhibit temporary antifertile activity in adult male animals.

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