Protective effect of Elateriospermum tapos in maternal obesity-induced deficit cognitive function of the offspring

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Azrina Zainal Abidin ◽  
Nurain Syahirah Rosli ◽  
Santhra Segaran ◽  
Azmiza Syawani Jasni ◽  
Hasnah Bahari
Keyword(s):  
Maternal Obesity ◽  
Memory Function ◽  
Positive Control ◽  
Negative Control ◽  
Diet Group ◽  
Normal Diet ◽  
Cafeteria Diet ◽  
Calorie Intake ◽  
Control Group ◽  
Sprague Dawley

Abstract Objectives Pre-pregnancy obesity is a serious epidemics concern that negatively affects the neurodevelopmental of the offspring. Elateriospermum tapos (E. tapos) commonly used to enhance weight loss in obesity treatment. This study was aimed to investigate the influence of E. tapos supplement in obese maternal prior pregnancy on the offspring’s bodyweight, lipid metabolism and memory function. Methods Thirty female Sprague Dawley rats were used. Six rats were assigned to the normal diet group (DND). The remaining rats were fed with a high-fat and cafeteria diet (HFCD) to generate obesity for 5 weeks. Obese rats were further divided into four groups: Negative Control (DNC; HFCD), Positive Control (DPC; Orlistat 200 mg/kg), treatment 1 (DTX1; E. tapos seed 200 mg/kg) and treatment 2 (DTX2, E. tapos shell 200 mg/kg) were given daily, for 6 weeks prior to mating. At weaning, offspring of both genders were designated into six groups according to their dam’s group (n=6/group). All groups were fed with a cafeteria diet except for the control group. Memory tasks for object and place recognition were conducted on offspring at eighth weeks of age. The offspring were cull at the 12th week of age for their blood sample. Results Both offspring genders from DTX2 significantly reduce bodyweight, calorie intake and triglyceride level. In memory tasks, offspring from DTX2 showed a significant increase in exploration rate in place test as compared to offspring from the DNC group. Conclusions This study highlights E. tapos shell as an anti-obesity agent and protecting memory deficit in obese dam’s offspring.

Ranitidine as an alcohol dehydrogenase inhibitor in acute methanol toxicity in rats

2009 ◽  
Vol 29 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Amal A El-Bakary ◽  
Sahar A El-Dakrory ◽  
Sohayla M Attalla ◽  
Nawal A Hasanein ◽  
Hala A Malek
Keyword(s):  
Alcohol Dehydrogenase ◽  
High Performance ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Blood Samples ◽  
Methanol Poisoning ◽  
Sprague Dawley Rats

Methanol poisoning is a hazardous intoxication characterized by visual impairment and formic acidemia. The therapy for methanol poisoning is alcohol dehydrogenase (ADH) inhibitors to prevent formate accumulation. Ranitidine has been considered to be an inhibitor of both gastric alcohol and hepatic aldehyde dehydrogenase enzymes. This study aimed at testing ranitidine as an antidote for methanol acute toxicity and comparing it with ethanol and 4-methyl pyrazole (4-MP). This study was conducted on 48 Sprague-Dawley rats, divided into 6 groups, with 8 rats in each group (one negative control group [C1], two positive control groups [C2, C3] and three test groups [1, 2 and 3]). C2, C3 and all test groups were exposed to nitrous oxide by inhalation, then, C3 group was given methanol (3 g/kg orally). The three test groups 1, 2 and 3 were given ethanol (0.5 g/kg orally), 4-MP (15 mg/kg intraperitoneally) and ranitidine (30 mg/kg intraperitoneally), respectively, 4 hours after giving methanol. Rats were sacrificed and heparinized, cardiac blood samples were collected for blood pH and bicarbonate. Non-heparinized blood samples were collected for formate levels by high performance liquid chromatography. Eye balls were enucleated for histological examination of the retina. Ranitidine corrected metabolic acidosis (p = .025), decreased formate levels (p = .014) and improved the histological findings in the retina induced by acute methanol toxicity.

scholarly journals Multi Floral Honey Has a Protective Effect against Mammary Cancer Induced by 7,12-Dimethylbenz(a)anthracene in Sprague Dawley Rats

2017 ◽  
Vol 9 (2) ◽  
pp. 196 ◽  
Author(s):  
Hamed R. Takruri ◽  
Maha S. Shomaf ◽  
Saida F. Shnaigat
Keyword(s):  
Protective Effect ◽  
Mammary Cancer ◽  
Histopathological Examination ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Light Cycle ◽  
Sprague Dawley ◽  
Dark Light

This research was conducted to study the protective effect of bee honey on the 7,12-dimethylbenz(a)anthracene (DMBA)- induced breast cancer in rat model. The study consisted of three groups: honey group, positive control group (PC), and negative control group (NC) to which the carcinogen was not administered. All rats were fed the diet recommended by the American Institute of Nutrition for growing rats (AIN-93G), with addition of honey (50 g/kg diet) to the honey group. All Rats were fed their diets ad libitum on 12 hours dark/light cycle. At the age of 50 days all rats in the honey and PC groups were gavaged once by the carcinogen DMBA with a dose of 80 mg/kg body Wt. After three weeks of carcinogen administration, rats were palpated weekly to detect any tumor growth. After 18 weeks, all rats were sacrificed. The palpable structures and the mammary glands along with associated lymph nodes were removed and fixed in saline formalin and prepared for histopathological examination. The results revealed that the honey group diet significantly (p < 0.05) reduced the incidence rate of mammary cancer, palpable tumor multiplicity, tumor size and weight compared to the PC group. In conclusion, multi floral honey has a protective effect against DMBA- induced mammary cancer in the initiation, promotion, and progression stages of DMBA-induced mammary carcinogenesis. However, further research is needed to reveal the mechanisms that might have contributed to the preventive effect of honey against mammary cancer.

Buprenorphine alleviation of pain does not compromise the rat monoarthritic pain model

2017 ◽  
Vol 16 (1) ◽  
pp. 167-167
Author(s):  
M.S. Berke ◽  
Klas S.P. Abelson
Keyword(s):  
Rat Model ◽  
Joint Stiffness ◽  
Positive Control ◽  
Negative Control ◽  
Pain Tolerance ◽  
Control Group ◽  
Mechanical Stimuli ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
A Minor

Abstract Aims This study investigated the effects of buprenorphine treatment on pain and welfare parameters and model specific parameters in a rat model of monoarthritis to eliminate unnecessary pain from this model. Methods 32 male Sprague Dawley rats were divided into four groups: (1) A negative control without arthritis receiving no analgesia. (2) A positive monoarthritic control group receiving no analgesia, but subcutaneous saline injections twice a day. (3) A positive control with monoarthritis receiving subcutaneous carprofen once a day and saline once a day. (4) A group with monoarthritis receiving subcutaneous buprenorphine twice a day. Monoarthritis was induced with an injection of 0.02 ml Complete Freund’s Adjuvant intra-articularly in the left tibiotarsal joint. Treatment with analgesia was initiated at day 15 and the rats were euthanized at day 23. Results The induced monoarthritis elicited a pronounced acute inflammation. Several parameters such as bodyweight, mobility, stance, joint-stiffness and lameness scores were affected. A marked mechanical hyperalgesia in the tarsal area was observed by Electronic Von Frey testing, but no severe compromise of the animal welfare was seen at any time. Signs of chronic development began to appear from day 10 after the monoarthritic induction. No significant change in serum cytokines and faecal corticosterone measurements was found after administration of buprenorphine. A minor decrease in body weight was seen, and a higher pain tolerance to mechanical stimuli was observed, indicating pain alleviation. The histological examination confirmed monoarthritic development in all monoarthritic rats and revealed periarticular lesions suggesting diffusion of adjuvant from intra-articular injection site to the periphery. Conclusions The study demonstrated that buprenorphine has an analgesic effect in the adjuvant induced monoarthritic rat model, without obvious interference with the development of arthritis.

scholarly journals Antioxidant and Hepatoprotective Activity of a New Tablets Formulation fromTamarindus indicaL.

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Jesús Rafael Rodriguez Amado ◽  
Ariadna Lafourcade Prada ◽  
Julio Cesar Escalona Arranz ◽  
Renato Pérez Rosés ◽  
Humberto Morris Quevedo ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Serum Levels ◽  
Hepatoprotective Activity ◽  
Negative Control ◽  
Diet Group ◽  
Control Group ◽  
Standard Diet ◽  
Tamarindus Indica ◽  
Sprague Dawley ◽  
Group I

Hepatotoxic chemicals damage liver cells primarily by producing reactive oxygen species. The decoction of the leaves ofTamarindus indicaL. is used for liver disorders. In this work we evaluated the hepatoprotective activity of a tablet formulation of this plant. Thirty-five Sprague Dawley rats were randomly divided into five groups (n=7). First group (I) is control group, fed with standard diet. Groups II to V (hepatotoxic groups) were subjected to a subcutaneous injection of CCl4(0.5 mL/kg). Group II was negative control, fed with standard diet; group III was subjected to administration of Silymarin 150 mg/kg and groups IV and V were treated with tablets in dose of 100 mg/kg and 200 mg/kg, respectively. Lipid peroxidation and the activity of superoxide dismutase, catalase, and reduced glutathione were evaluated. Serum levels of alanine aminotransferase, aspartate aminotransferase, gamma-glutamine transferase, alkaline phosphatase, and a lipid profile were evaluated too. The tablets inhibit lipid peroxidation. The redox balance (SOD-CAT-GSH) remains normal in the experimental groups treated with tablets. The liver function using dose of 200 mg/kg of tablets was better than the other experimental groups. These results justify, scientifically, the ethnobotanical use of the leaves ofTamarindus indicaL.

scholarly journals The Effect of Tempeh Gembus Variations to Serum Levels of High Sensitivity C-Reactive Protein (hsCRP) and Serum Levels of Fibrinogen of Sprague Dawley Rats with Aterogenic Diet

2018 ◽  
Vol 25 (1) ◽  
pp. 91-97 ◽  
Author(s):  
Partika Kharunia Dewi ◽  
Diana Nur Afifah ◽  
Ninik Rustanti ◽  
Mohammad Sulchan ◽  
Gemala Anjani
Keyword(s):  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Atherogenic Diet ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Standard Diet ◽  
Sprague Dawley ◽  
Significant Difference

Abstract Background and aims: Cardiovascular diseases are widespread and causes many deaths in the world. The concentration of acute phase protein: C-reactive protein (CRP) and fibrinogen will rise dramatically when inflammation happens, which that can be used as an early marker of cardiovascular disease risk. Tempeh gembus contains fiber, unsaturated fatty acids and isoflavones are believed to reduce the inflammatory reaction. The aim of the study was to determinate the effect of tempeh gembus variations to levels of hcCRP and levels of fibrinogen of Sprague Dawley rats with atherogenic diet. Material and methods: This study was quasi-experimental with posttest only randomized control group design using 35 Sprague Dawley mice. The rats were randomized into 5 groups: negative control group given the standard diet, the positive control group given standard diet and atherogenic diet, and three treatment groups were given the standard diet, atherogenic diet and variation of tempeh gembus (tempeh gembus, heated tempeh gembus and tempeh gembus with bromelain enzyme) for 28 days. Serum levels of hsCRP and fibrinogen examined using ELISA (Enzyme-linked Immunosorbent Assay). Results and conclusions: The administration of tempeh gembus with bromelain enzyme is the most effective treatment for hsCRP serum level indicated a significant difference (p=0.028) between the negative control group, positive control group and first group with the third group. Fibrinogen serum levels showed significant differences in all treatment groups (p =0.042), administration of tempeh gembus with bromelain enzyme is the most effective treatment is shown by a significant difference between the negative control group and the positive control group with third group. The administration of tempeh gembus with bromelain enzyme for 28 days can reduce the serum levels of hsCRP and fibrinogen on rats significantly.

scholarly journals Hepatoprotective Activity of Ethanol Extract of Figs Leaves (Ficus carica L.) with SGOT & SGPT Parameters in Sprague Dawley Female Rats Induced by Paracetamol

2021 ◽  
Vol 7 (2) ◽  
pp. 110-119
Author(s):  
Muhammad Fariez Kurniawan ◽  
Herdita Nugraheny Kusuma Wardany
Keyword(s):  
Ethanol Extract ◽  
Test Dose ◽  
Hepatoprotective Activity ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Female Rats ◽  
Control Group ◽  
Sprague Dawley ◽  
Serum Glutamic Oxaloacetic Transaminase

Background: Long-term usage of paracetamol damages liver cells characterized by the increasing levels of Serum Glutamic Pyruvic Transaminase (SGPT) and Serum Glutamic Oxaloacetic Transaminase (SGOT). Figs (Ficus carica L.) leaves contain high flavonoid compounds that able to act as hepatoprotector agents which inhibited the process of liver damage. Objectives: This study aims to determine the dose characteristics and hepatoprotective activity of figs (Ficus carica L.) leaves extract. Material and Methods: The hepatoprotective activity of figs extract (Ficus carica L.) was determined by measuring SGPT & SGOT levels in rat blood. 42 rats were prepared, divided into 7 groups: normal control group, negative control given paracetamol with a dose 40 mg/200 g BW, positive control given hepamax®, base control given Na CMC, and 3 test dose groups given figs leaves extract with a dose of 40 mg/200 g BW, 80 mg BW/200 g BW and 160 mg/200 g BW. The treatment was carried out for 14 consecutive days. Paracetamol was given for 14 days, while positive control, 3 test dose groups and base control were given on day 7 to day 14.  Rats blood samples were taken through the orbital sinuses on day 1, day 5, day 10 and day 14 after treatment began. SGPT & SGOT levels were determined using spectrophotometry with analytical methods using specific SGPT & SGOT reagent kits.  Results: The results showed that the leaves extract of figs (Ficus carica L.) proved to be able to reduce the levels of SGPT & SGOT with the most potent dosage was 40 mg/200 g BW. However, the reduction of SGPT & SGOT levels were not significantly different from other treatments. Conclusions: Figs (Ficus carica L.) leaves extract showed activity as a hepatoprotective agent, based on the reduction of SGPT & SGOT levels in rat after 14 days of treatment.

scholarly journals EFFICACY OF A STANDARDIZED ETHANOL EXTRACT OF ROSELLE CALYX IN THE TREATMENT OF ORAL MUCOSA ULCERATION (IN VIVO)

2017 ◽  
Vol 10 (17) ◽  
pp. 183
Author(s):  
Reni Jayantini ◽  
Dewi Fatma Suniarti ◽  
Agoeng T Sarwono
Keyword(s):  
Topical Application ◽  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Ethanol Extracts ◽  
Inflammation Score

 Objectives: To analyze the effectiveness of the topical application of a standardized ethanol extract of roselle calyx in the treatment of oral mucous ulceration.Methods: Twelve Sprague Dawley rats were divided into two groups, with oral mucous ulcer being induced in all the rats. The ulcer diameter, reddish color, and the swollen membrane around the ulcer, as well as the inflammation score of the ulcer, were recorded for all animals. The negative control group was treated with aquades, while the positive control group was treated with 0.1% triamcinolone acetonide. The treatment group received the topical application of 7.5% and 15% standardized ethanol extract of roselle calyx twice a day for 3 or 7 days. On the 4th day, six rats were sacrificed, while the remaining six rats continued to receive treatment as before. On the 8th day, all the rats were sacrificed. Specimens were fixed, and histology slides were made. Further, microscopic slides were observed and scored.Result: Both the 7.5% and 15% standardized ethanol extracts of roselle calyx were found to reduce the ulcer diameter and inflammation score. No swelling or redness was observed.Conclusions: The application of 7.5% standardized ethanol extract of roselle calyx for 7 days is equally as effective as the application of 0.1% triamcinolone acetonides, except in terms of the decrease in the inflammation score.

scholarly journals Oral Administration of Tualang and Manuka Honeys Modulates Breast Cancer Progression in Sprague-Dawley Rats Model

2017 ◽  
Vol 2017 ◽  
pp. 1-15 ◽  
Author(s):  
Sarfraz Ahmed ◽  
Siti Amrah Sulaiman ◽  
Nor Hayati Othman
Keyword(s):  
Breast Cancer ◽  
Cancer Progression ◽  
Estrogenic Activity ◽  
Tumour Size ◽  
Virgin Female ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Therapeutic Effects ◽  
Sprague Dawley

Breast cancer has been recognized as the leading cause of death in women worldwide. Research has shown the importance of complementary and alternative therapies in cancer. In this study, we investigated the antitumoural therapeutic effects of Malaysian Tualang honey (TH) and Australian/New Zealand Manuka honey (MH) against breast cancer in rats. Thirty syngeneic virgin female Sprague-Dawley (SD) rats were induced by the carcinogen 1-methyl-1-nitrosourea (MNU) 80 mg/kg. The treatment started when first palpable tumour reached 10–12 mm in size by dividing rats into following groups: Group 0 (negative control); Group 1 (positive control); and Groups 2 and 3 which received 1.0 g/kg body weight/day of TH and MH, respectively, for 120 days. The data demonstrate that cancer masses in TH and MH treated groups showed a lower median tumour size, weight, and multiplicity compared with the nontreated positive control (p<0.05). Treatment also showed a dramatic slower growth rate (up to 70.82%) compared with the nontreated control (0%) (p<0.05). The antitumoural effect was mediated through modulation of tumour growth, tumour grading, estrogenic activity, and haematological parameters. Our findings demonstrate that systemic administration of TH and MH increases the susceptibility of expression of proapoptotic proteins (Apaf-1, Caspase-9, IFN-γ, IFNGR1, and p53) and decreases the expression of antiapoptotic proteins (TNF-α, COX-2, and Bcl-xL 1) in its mechanism of action. This highlights a potential novel role for TH and MH in alleviating breast cancer.

scholarly journals Is traditional herbal mix composition effective on wound healing?

2021 ◽  
Vol 8 (1) ◽  
pp. 29-38
Author(s):  
Serap Gokce Eskin ◽  
Yucel Basimoglu Koca ◽  
Serdal Ogut
Keyword(s):  
Wound Healing ◽  
Negative Control Group ◽  
Grape Seed ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Wound Model ◽  
Experimental Group

Objective: Wound healing is a dynamic process that includes biochemical and physiological phenomena. Studies confirm that plants are used in wound healing to a great extent.  Antique oil (HBX 2371) has been produced as a traditional herbal mixed. This herbal mixture contains sesame oil, thyme oil, olive leaf, fig seed, grape seed, turmeric, and cinnamon. We evaluated traditional plant mixed oil ( Antique oil) for its wound-healing activity using an excision wound model in rats. Methods: The study used a preclinical, in vivo experimental, and analytical design. In the study, a total of 21 Sprague-Dawley rats, including 7 as experimental group, 7 as negative-control group, and 7 as positive-control group, were used. After the ischemic wound was created in experimental animals, the research data relating to the histochemical changes and biochemical parameters of the wound healing parameters were collected Results: A to research in experimental group fibroblast count, collagen density, fat cells, epithelization scores higher than the other groups and inflammatory cell density lower than the others. According to the results of the study, the highest TAC value (3.94 ± 0.21) was determined in the experimental group and results showed that antique oil administration decreased the TOC value. Conclusion: In conclusion, it was observed that antique oil prevented the wound changes induced by the incision, increased the repair of the epidermal and dermal structure in a short time, increased the antioxidant level, and decreased the oxidation level. Clinical studies are recommended.

scholarly journals THE ROLE OF VITAMIN E (α-TOCOPHEROL) ON TESTOSTERONE LEVEL IN SPRAGUE DAWLEY RATS FOLLOWING CISPLATIN TREATMENT

2019 ◽  
Vol 26 (1) ◽  
Author(s):  
Dian Kartika Rezia ◽  
Lukman Hakim ◽  
Wahjoe Djatisoesanto
Keyword(s):  
Vitamin E ◽  
Adult Male ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Sprague Dawley ◽  
Testosterone Levels ◽  
White Rats ◽  
Sprague Dawley Rats

Objective: To observe the difference of testosterone levels in adult male Sprague Dawley rats treated with combination of cisplatin and vitamin E compared to those treated with cisplatin only. Material & Methods: We used 24 adult male Sprague Dawley rats weight 200–300 grams and randomly assigned into 4 groups (n=6). Rats in negative control group (NC) were given intraperitoneal normal saline injection, while the positive control (PC) group were injected with cisplatin 5 mg/kgBW at the end of the 3rd week. Two other groups, P1 and P2, were injected with cisplatin 5 mg/kgBW and given vitamin E orally 50 mg/kgBW and 200 mg/kgBW, respectively. Cardiac blood was aspirated at the end of the 7th week and processed for analysis of testosterone levels. Results: We recorded a significantly lower testosterone levels in rats treated only with cisplatin 5 mg/kgBW (CP) compared to those in CN group (p=0.006), and those receiving combination of cisplatin and vitamin E 50 mg/kgBW (p=0.003) and 200 mg/kgBW (p=0.001). Though not significant, testosterone levels were higher in P2 group than in P1 group (p=0.702). Conclusion: Exposure to cisplatin can lower testosterone levels in white rats, and the administration of vitamin E gives protection against such effect. 

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