Eco friendly synthesis and characterization of zinc oxide nanoparticles from Aegle marmelos and its cytotoxicity effects on MCF-7 cell lines

2021 ◽  
Vol 6 (1) ◽  
pp. 44-51
Author(s):  
Manikandan Dhayalan ◽  
Malathi Selvaraj ◽  
Kumar B Karthick ◽  
Riyaz S.U. Mohammed ◽  
Mika Sillanpää
Keyword(s):  
Zinc Oxide ◽  
Cell Lines ◽  
Absorption Peak ◽  
Aegle Marmelos ◽  
Zno Nps ◽  
Pure Zinc ◽  
Characteristic Absorption ◽  
Characteristic Absorption Peak ◽  
Vis Spectroscopy ◽  
Mcf 7

Abstract An attempt was made to synthesize zinc oxide gum white nanoparticles (ZnO-GWNPs) by the greenway approach using Aegle marmelos (Bael fruit) juice extract as a capping and reducing agent. Synthesis of ZnO-GWNPs by greener approach is safer, more economical, more energy-efficient, eco-friendlier, and less toxic than chemically synthesized counterparts. The optical properties of the ZnO-GWNPs were ascertained through UV-Vis spectroscopy, Fourier Transform-Infrared (FT-IR), X-ray diffraction (XRD), High-resolution transmittance electron microscopy (HRTEM). A characteristic absorption peak at 385nm confirmed the presence of ZnO-GWNP using UV-Vis spectroscopy. FTIR spectrum revealed that the characteristic absorption peak of the Zn-O bond was observed at 467 cm-1. The XRD result for the ZnO showed the tendency of the three most intense diffraction peaks. The average crystallite size ZnO NPs at scattering angle (2θ) 22.89 and 32.15 was 39.14 and 26.08 nm and it showed the presence of miller indices of (100), (002), (101), (102) respectively. The EDX spectrum gave strong signals for zinc and oxygen indicating the occurrence of the nanoparticles in their oxide form rather than the pure zinc form. The SEM image showed the surface morphology of ZnO-GW NPs and the HR-TEM image showed the crystalline nature of ZnO-GW NPs. Cytotoxicity study of ZnO-GW NPs was determined against MCF-7 cell lines and the IC50 values were found to be 40 µg/mL and 60 µg/mL at 24 h and 48 h respectively.

scholarly journals Structural characterization, antioxidant and cytotoxic effects of iron nanoparticles synthesized using Asphodelus aestivus Brot. aqueous extract

2020 ◽  
Vol 9 (1) ◽  
pp. 153-163 ◽  
Author(s):  
Burcu Sumer Tuzun ◽  
Tugce Fafal ◽  
Pelin Tastan ◽  
Bijen Kivcak ◽  
Besra Ozmen Yelken ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Receptor Gene ◽  
Control Cell ◽  
Antioxidant Properties ◽  
Gene Expressions ◽  
Vis Spectroscopy ◽  
Mcf 7

AbstractASP was used to synthesize FeNPA. They were characterized by UV-vis spectroscopy, FT-IR, TEM, SEM, XRD and ZP. The aim of this study was to evaluate in vitro cytotoxic activity and antioxidant acitivities of FeNPA and ASP. The antioxidant properties were evaluated using DPPH, ABTS+ and H2O2 assays. FeNPA had higher antioxidant activity comparing to ASP according to DPPH (IC50: 3.48 μg/mL) and ABTS+ (60.52%) assays. Anti-cancer activities of FeNPA and ASP were investigated in breast cancer, melanoma and control cell lines. FeNPA was more cytotoxic than ASP in MCF-7, MeWo, CHL-1, and HEL 299 cells. FeNPA had shown that mitochondria induce apoptosis through stress in MDA-MB-231, and cells MeWo. ASP also induced apoptosis 2.23-fold in MCF-7 cells. Progesterone receptor gene expression showed a 10-fold increase in a hormone-dependent MCF-7 cell line in ASP, and FeNPA treatment. Expressions of BCL6, CXCL12, DNAJC15, RB1 and TPM1 in melanoma cancer cell lines were significantly increased in ASP and FeNPA administration. It had been shown that FeNPA regulates gene expressions that may be considered important in terms of prognosis in breast cancer and melanoma cell lines and it is suggested that gene expressions regulated by FeNPA are also evaluated in animal models in vivo.

scholarly journals Crotalaria verrucosa Leaf Extract Mediated Synthesis of Zinc Oxide Nanoparticles: Assessment of Antimicrobial and Anticancer Activity

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 4896
Author(s):  
Siva Sankar Sana ◽  
Divya Vishambhar Kumbhakar ◽  
Akbar Pasha ◽  
Smita C. Pawar ◽  
Andrews Nirmala Grace ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Cell Migration ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Leaf Extract ◽  
Positive Control ◽  
Ftir Spectra ◽  
Total Phenolic ◽  
Oxide Nanoparticles ◽  
Zno Nps

In this work, we present an ecofriendly, non-hazardous, green synthesis of zinc oxide nanoparticles (ZnO NPs) by leaf extract of Crotalaria verrucosa (C. verrucosa). Total phenolic content, total flavonoid and total protein contents of C. verrucosa were determined. Further, synthesized ZnO NPs was characterized by UV–visible spectroscopy (UV-vis), X-ray diffractometer (XRD), Fourier transform infra-red (FTIR) Spectra, transmission electron microscope (TEM), and Dynamic light scattering (DLS) analysis. UV-vis shows peak at 375 nm which is unique to ZnO NPs. XRD analysis demonstrates the hexagonal phase structures of ZnO NPs. FTIR spectra demonstrates the molecules and bondings associated with the synthesized ZnO NPs and assures the role of phytochemical compounds of C. verrucosa in reduction and capping of ZnO NPs. TEM image exhibits that the prepared ZnO NPs is hexagonal shaped and in size ranged between 16 to 38 nm which is confirmed by DLS. Thermo-gravimetric analysis (TGA) was performed to determine the thermal stability of biosynthesized nanoparticles during calcination. The prepared ZnO NPs showed significant antibacterial potentiality against Gram-positive (S. aureus) and Gram-negative (Proteus vulgaris, Klebsiella pneumoniae, and Escherichia coli) pathogenic bacteria and SEM image shows the generalized mechanism of action in bacterial cell after NPs internalization. In addition, NPs are also found to be effective against the studied cancer cell lines for which cytotoxicity was assessed using MTT assay and results demonstrate highest growth of inhibition at the concentration of 100 µg/mL with IC50 value at 7.07 µg/mL for HeLa and 6.30 µg/mL for DU145 cell lines, in contrast to positive control (C. verrucosa leaf extract) with IC50 of 22.30 µg/mL on HeLa cells and 15.72 µg/mL on DU145 cells. Also, DAPI staining was performed in order to determine the effect on nuclear material due to ZnO NPs treatment in the studied cell lines taking leaf extract as positive control and untreated negative control for comparison. Cell migration assay was evaluated to determine the direct influence of NPs on metastasis that is potential suppression capacity of NPs to tumor cell migration. Outcome of the synthesized ZnO NPs using C. verrucosa shows antimicrobial activity against studied microbes, also cytotoxicity, apoptotic mediated DNA damage and antiproliferative potentiality in the studied carcinoma cells and hence, can be further used in biomedical, pharmaceutical and food processing industries as an effective antimicrobial and anti-cancerous agent.

Research on Performance of Vetier (Vetiveria zizanioides) Cellulose Micro/Nano Fibrils Isolated by High Intensity Ultrasonication

2011 ◽  
Vol 393-395 ◽  
pp. 1405-1408
Author(s):  
Xin Wang ◽  
Yan Wu ◽  
Jin Tian Huang
Keyword(s):  
High Intensity ◽  
Absorption Peak ◽  
Wave Number ◽  
Vetiveria Zizanioides ◽  
Relative Crystallinity ◽  
Characteristic Absorption ◽  
Basic Properties ◽  
Characteristic Absorption Peak

The cellulose micro/nano fibrils of Vetier (Vetiveria zizanioides) were isolated by high intensity ultrasonication in this paper. The basic properties of micro/nano fibrils were evaluated by WAXD and FTIR. The results showed that the relative crystallinity decreased when treated by high intensity ultrasonication. The characteristic absorption peak moved to higher wave number and the type of group increased with increasing the vibration of ultrasonication. The characteristic absorption peak moved to higher wave number with increasing treated time, however, the effect was insignificant.

scholarly journals Optimization and green synthesis of zinc oxide nanoparticle using Garcinia cambogia leaf and evaluation of their antioxidant and anticancer property in kidney cancer (A498) cell lines

Biomedicine ◽  
2021 ◽  
Vol 41 (2) ◽  
pp. 206-222
Author(s):  
Varsha Jayakar ◽  
Vinayak Lokapur ◽  
B. R. Nityasree ◽  
Raju Krishna Chalannavar ◽  
Lyned D. Lasrado ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Green Synthesis ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Zno Nps ◽  
Xtt Assay ◽  
Garcinia Cambogia ◽  
Hek 293 ◽  
A498 Cell ◽  
Hek 293 Cell Line

Introduction and Aim: In 21stcentury, nanomedicine has turned out to be an emergent modulus operation for the diagnosis and treatment of cancer. The current study includes the optimization and green synthesis of zinc oxide nanoparticles from the leaves of Garcinia cambogia and interpretation of its antioxidant and anticancer activity.   Materials and Methods: Synthesized ZnO NPs were investigated by UV–vis, FTIR, XRD, SEM, EDX, HR-TEM, SAED, particle size distribution and DLS analysis. Furthermore, different antioxidant assays were conducted for evaluating the antioxidant activity of (ZnO NPs) and its anticancer activity using (A498) cell lines along with non-cancerous (HEK-293) cell line using MTT assay and XTT assay.   Results: The UV–visible spectrum showed an absorption peak at 277 nm that reflects surface plasmon resonance (SPR) ZnONPs. The presence of various functional groups was confirmed by FTIR analysis. XRD patterns indicated that the ZnO NPs had a single-phase nature with the wurtzite structure. SEM, TEM pictures confirmed the size and shape of synthesized Nanoparticles ranges of 11–32 nm and mostly hexagonal forms. The Zeta potential value of 57.9 mV revealed the surface charge of green synthesized ZnONPs. The cytotoxicity of the ZnO NPs with various concentrations (6.25 to 200 µg/mL) was measured in (A498) cell lines along with non-cancerous (HEK-293) cell line. After the exposure, to MTT and XTT assay, cellular morphology studies showed that the cancer cell viability decreased with increasing ZnO NP concentration.   Conclusion: The synthesized ZnONPs via a green synthetic route could be developed as anti-cancer agent against cancer cells.

Sunflower Assisted Bio-derived ZnO-NPs as an Efficient Nanocatalyst for Synthesis of Novel Quinazolines with Highly Antioxidant Activities

2021 ◽  
Author(s):  
Balaji GL ◽  
S. Mahesh ◽  
Boya Palajonnala Narasaiah ◽  
Sadegh Rostamnia ◽  
Harihara Padhy ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Zinc Oxide Nanoparticles ◽  
Antioxidant Activities ◽  
Present Report ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Oxide Nanoparticles ◽  
Zno Nps ◽  
Green Method ◽  
Vis Spectroscopy

Abstract The present report an environmental benign route for the fabrication of Zinc Oxide nanoparticles (ZnO-NPs) using sunflower leaf aqueous extract at room temperature. This was an green method is a rapid biogenic and offers few advantages over the chemical and physical procedures, as it is an easy and fast, eco-friendly and does not involve any costly chemicals as well as hazardous chemicals, harmful solvent. The synthesized ZnO-NPs were characterized using different technique such as UV-Visible spectroscopy. The formation of ZnO-NPs was confirmed by Surface Plasmon Resonance (SRP) at 344 nm using UV-Vis spectroscopy. The leaf extract act as a source of phyto-chemicals and are the primarily responsible for the reduction and then formation of stable Zinc Oxide nanoparticles by the characteristic functional groups of extract and synthesized ZnO-NPs were identified by FTIR spectroscopy. Crystalline nature of ZnO-NPs was confirmed by powder –XRD. Size and morphology were measured by HR-TEM analysis. Stability of the nanoparticles is represented by DLS and TGA analysis. The synthesized ZnO-NPs has been found good and efficient catalyst for the synthesis of novel 1,2-dihydroquinazoline derivatives under green method via one pot reaction of 2-amino benzophenone, 1,3-diphenyl-1 H -pyrazole carbaldehydes and ammonium acetate. The synthesized compounds (4a-o) were characterized by 1 HNMR, 13 CNMR and HRMS spectra and further screened for radical scavenging activity. Among all the compounds showed good percentage of inhibition against standard ascorbic acid. The synthesized ZnO nanoparticles showed good antioxidant activity and also act as a good promising material for catalytic agent.

scholarly journals Characterization and biological activities of synthesized zinc oxide nanoparticles using the extract of Acantholimon serotinum

2020 ◽  
Vol 9 (1) ◽  
pp. 722-733
Author(s):  
Mohammad Reza Karimzadeh ◽  
Sara Soltanian ◽  
Mahboubeh Sheikhbahaei ◽  
Neda Mohamadi
Keyword(s):  
Cell Lines ◽  
Biological Activities ◽  
Fold Increase ◽  
Diffusion Method ◽  
Ros Generation ◽  
Zno Nps ◽  
Human Colon Cancer ◽  
Hek 293 ◽  
Vis Spectroscopy ◽  
Apoptosis Gene

AbstractThe present study reports the synthesis of ZnO-NPs using Acantholimon serotinum extracts followed by characterization and evaluation of biological activities. Field emission scanning electron microscope revealed irregular spherical morphology with a size in the range of 20–80 nm. The X-ray diffraction analysis confirmed the synthesis of highly pure ZnO NPs with a hexagonal shape and a crystalline size of 16.3 nm. The UV-Vis spectroscopy indicates the synthesis of ZnO-NPs. FT-IR confirmed the presence of phytocomponents in the plant extract, which was responsible for nanoparticle synthesis. According to MTT results, the biosynthesized ZnO-NPs showed cytotoxic effects on human colon cancer Caco-2 (IC50: 61 µg/mL), neuroblastoma SH-SY5Y (IC50: 42 µg/mL), breast cancer MDA-MB-231 (IC50: 24 µg/mL), and embryonic kidney HEK-293 (IC50: 60 µg/mL) cell lines. Significant reactive oxygen species (ROS) generation was measured by the DCFH-DA assay after 24 h incubation with ZnO-NPs (200 µg/mL). ZnO-NPs caused apoptotic and necrotic effects on cells, which was confirmed by Annexin V-PE/7-AAD staining and 6.8-fold increase in pro-apoptosis gene Bax and 178-fold decrease in anti-apoptosis gene Bcl-2. The well diffusion method did not show effective growth inhibition activities of the ZnO-NPs against bacteria. In conclusion, the ZnO-NPs induce cytotoxicity in cell lines through ROS generation and oxidative stress.

scholarly journals Greener Synthesis of Zinc Oxide Nanoparticles: Characterization and Multifaceted Applications

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4198
Author(s):  
Ali Aldalbahi ◽  
Seham Alterary ◽  
Ruba Ali Abdullrahman Almoghim ◽  
Manal A. Awad ◽  
Noura S. Aldosari ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Zinc Oxide ◽  
Cell Line ◽  
Zinc Oxide Nanoparticles ◽  
Diffusion Method ◽  
Oxide Nanoparticles ◽  
Eosin Y ◽  
Zno Nps ◽  
Vitro Assay ◽  
Vis Spectroscopy

Nanoparticles (NPs) have unique properties compared to their bulk counterparts, and they have potentials for various applications in many fields of life science. Green-synthesized NPs have garnered considerable interest due to their inherent features such as rapidity, eco-friendliness and cost-effectiveness. Zinc oxide nanoparticles (ZnO NPs) were synthesized using an aqueous extract of Kalanchoe blossfeldiana as a reducing agent. The resulting nanoparticles were characterized via X-ray diffraction (XRD), dynamic light scattering (DLS), UV-Vis spectroscopy, photoluminescence (PL), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive spectroscopy (EDS). The antimicrobial potential of the synthesized ZnO NPs against bacterial and fungal strains was examined by the disk diffusion method, and they showed a promising antibacterial and antifungal potential. The catalytic activity of the synthesized ZnO NPs in reducing methylene blue (MB) and eosin was studied via UV-Vis spectroscopy. The decolorization percentages of the MB and Eosin Y dyes were 84% and 94%, respectively, which indicate an efficient degradation of the ZnO NPs. In addition, the cytotoxic activity of the ZnO NPs on the HeLa cell line was evaluated via in vitro assay. The MTT assay results demonstrate a potent cytotoxic effect of the ZnO NPs against the HeLa cancer cell line.

The Effect of pH on Zinc Oxide Nanoparticles Characteristics Synthesized from Banana Peel Extract

2019 ◽  
Vol 797 ◽  
pp. 271-279 ◽  
Author(s):  
R.A. Abdol Aziz ◽  
Siti Fatma Abd Karim ◽  
Nor Asikin Rosli
Keyword(s):  
Zinc Oxide ◽  
Crystallite Size ◽  
Zno Nanoparticles ◽  
Absorption Peak ◽  
Raw Material ◽  
Banana Peel ◽  
Zno Nps ◽  
Ability To Act ◽  
Bet Analysis ◽  
Optimum Ph

In this study, Zinc Oxide (ZnO) Nanoparticles (NPs) were synthesized from banana peels (Jackfruit banana) extract (BPE) at different pH condition. The samples were then characterized to identify the optimum pH condition for producing ZnO NPs and at the same time determine the crystallite and particles size of ZnO. This paper covered a section of green chemistry since green application has become an attention nowadays. Slo-gel method is the method used to synthesize the ZnO NPS because the advantages in terms of eco-friendly, less time consumption, cost effective and easy to apply. BPE is one of raw material that has the ability to act as stabilizer and reducing agent. The samples were characterized using Fourier Transform Infrared Red (FTIR) Spectroscopy, UV-visible spectrometer (UV-Vis), X-ray diffraction (XRD) and Brunaner-Emmett-Teller (BET). It was found that the presence of ZnO were recorded from FTIR spectra at wavenumber 350-390 cm-1for all samples which indicating the presence of ZnO bond. The UV-Vis spectrometer was recorded to observe the absorption peak, the highest absorption peak at 367 nm and the band gap was 3.38 Ev at pH 12. XRD analysis showed the ZnO nanoparticles formed to have hexagonal wurtzite structure and the crystallite size between 16 to 23 nm and the smallest crystallite size was smallest at pH 12. BET analysis showed that the surface area of ZnO NPs between 15 to 53 m2/g and the average particles size of ZnO NPs between 20 to 66 nm. As a conclusion, ZnO NPs can be produced from BPE at optimum pH of 12.

scholarly journals A POTENT CYTOTOXICITY AND ANTIMICROBIAL ACTIVITY OF ZINC OXIDE NANOPARTICLES SYNTHESIZED BY LEAF OF IPOMOEA PES-CAPRAE (L.) R. BR.

2019 ◽  
pp. 111-117
Author(s):  
RAMESH A ◽  
SUNDARRAJ P ◽  
BALAMANI J
Keyword(s):  
Antimicrobial Activity ◽  
Zinc Oxide ◽  
Cell Lines ◽  
Zinc Oxide Nanoparticles ◽  
Assay Method ◽  
Oxide Nanoparticles ◽  
Visible Spectroscopy ◽  
Zno Nps ◽  
Cytotoxicity Activity ◽  
Uv Visible

Objective: The present study was conducted to investigate the cytotoxicity and antimicrobial activity of zinc oxide nanoparticles (ZnO NPs) synthesized as eco-friendly technique from the leaf extract of Ipomoea pes-caprae (L.) R. Br. against human lung adenocarcinoma (A549), brain tumor (U87) cells, and human pathogens Salmonella typhi, Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, and Bacillus subtilis. Materials and Methods: The work was carried out with varying precursor (plant extract) volume to optimize the synthesis of ZnO NPs and it was confirmed by ultraviolet (UV)-visible spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) analysis, scanning electron microscopy, and atomic force microscope (AFM) characterization techniques and evaluate its cytotoxicity activity by 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl--tetrazolium bromide assay method, antimicrobial activity by disk diffusion method. Results: A peak at 320 nm with maximum intensity was observed at temperature of 80°C with pH of 8.0 in UV-visible spectroscopy confirmed the formation of ZnO NPs and we calculate the size of ZnO NPs from XRD data found as 15.8 nm. The FTIR analysis evaluated that the presence of different functional groups is carboxyl, amine, and phenolic compounds of leaves extract which are involved in the reduction of zinc ions and acts as capping the ZnO NPs. AFM microgram confirms that ZnO NPs were in nanorange and spherical in nature. The cytotoxicity activity of A549 and U87 cell lines treated with various concentrations of ZnO NPs showed a dose-dependent increase in cell inhibition and the half maximal inhibitory concentration value was calculated to be 7.8 μg/ml. The antibacterial activity of selected pathogens shows higher zone of inhibition. Conclusion: The present study reveals that synthesized ZnO NPs capping with various bioactive compounds present in the leaf of I. pes-caprae show promising activity of cancer cell lines and antimicrobial agents; hence, further detailed study may lead to develop at a novel phytomedicine for the anticancer and antimicrobial drugs.

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