Clinal variation in Pinus hartwegii populations and its application for adaptation to climate change

AbstractIn order to determine the genetic variation among populations of Pinus hartwegii along altitudinal gradients, and the possible adaptive decoupling due to climatic change, a common garden test was set up with 13 provenances collected along an altitudinal transect (3150 to 3750 masl; collection sites were every 50 m of altitudinal difference) from Pico de Tancítaro, Michoacán, west-central Mexico. The test evaluated trees aged 4.5 to 5.5 years, and contemporary and future (year 2030; six model-emission scenarios) climate variables were estimated. We found significant (P<0.005) differences among populations in total elongation, final seedling height, basal diameter, rate of elongation, dry weight of needles, branches and stem, total dry weight and first principal component. We found a significant association between population performance, expressed as first principal component values, and their climate, where the best climatic predictor was the frost free period (r2=0.90, P<0.0001). This result indicates that the patterning of genetic differentiation among populations is strongly associated with a climatic and altitudinal gradient. We propose a re-delineation of fixed climatic and altitudinal seed zoning for seed and seedling movement, based on intervals of 26 days of difference of frost free period: Zone 1, from 240 to 214 days of frost free period or 12 to 11°C or 3150 to 3350 masl; Zone 2, 214 to 188 days or 11 to 10°C or 3350 to 3550 masl; and Zone 3, 188 to 162 days or 10 to 9°C or 3550 a 3750 masl. Projecting at what altitude will occur the same frost free period values by year 2030, we suggested that populations should be shifted 400 m attitudinally upward, through a program of assisted migration, aiming to realign the populations to the climate for which they are adapted. Seedling deployment should use future (year 2030) fixed seed zones, with the same projected climatic interval values than contemporary climate, although at the following altitudinal limits: Zone 12030, from 3550 to 3750 masl; Zone 22030, 3750 to 3950 masl; and Zone 32030, 3950 to 4150 masl.

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Leaf Canopy Layers Affect Spectral Reflectance in Silver Birch

Remote Sensing ◽

10.3390/rs11242884 ◽

2019 ◽

Vol 11 (24) ◽

pp. 2884 ◽

Cited By ~ 5

Author(s):

Maya Deepak ◽

Sarita Keski-Saari ◽

Laure Fauch ◽

Lars Granlund ◽

Elina Oksanen ◽

...

Keyword(s):

Principal Component ◽

Common Garden ◽

Dry Weight ◽

Leaf Traits ◽

Tree Canopy ◽

Canopy Reflectance ◽

Canopy Layer ◽

Leaf Reflectance ◽

Individual Trees ◽

Reflectance Indices

The availability of light within the tree canopy affects various leaf traits and leaf reflectance. We determined the leaf reflectance variation from 400 nm to 2500 nm among three canopy layers and cardinal directions of three genetically identical cloned silver birches growing at the same common garden site. The variation in the canopy layer was evident in the principal component analysis (PCA), and the influential wavelengths responsible for variation were identified using the variable importance in projection (VIP) based on partial least squares discriminant analysis (PLS-DA). Leaf traits, such as chlorophyll, nitrogen, dry weight, and specific leaf area (SLA), also showed significant variation among the canopy layers. We found a shift in the red edge inflection point (REIP) for the canopy layers. The canopy layers contribute to the variability in the reflectance indices. We conclude that the largest variation was among the canopy layers, whereas the differences among individual trees to the leaf reflectance were relatively small. This implies that within-tree variation due to the canopy layer should be taken into account in the estimation of intraspecific variation in the canopy reflectance.

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Landscape patterns of phenotypic variation and population structuring in a selfing grass, Elymus glaucus (blue wildrye)

Canadian Journal of Botany ◽

10.1139/b04-141 ◽

2004 ◽

Vol 82 (12) ◽

pp. 1776-1789 ◽

Cited By ~ 30

Author(s):

Vicky J Erickson ◽

Nancy L Mandel ◽

Frank C Sorensen

Keyword(s):

Genetic Variation ◽

Principal Component ◽

Common Garden ◽

Primary Objective ◽

Landscape Patterns ◽

Clinal Variation ◽

Phenotypic Variance ◽

Seed Transfer ◽

Wide Range ◽

Elymus Glaucus

Source-related phenotypic variance was investigated in a common garden study of populations of Elymus glaucus Buckley (blue wildrye) from the Blue Mountain Ecological Province of northeastern Oregon and adjoining Washington. The primary objective of this study was to assess geographic patterns of potentially adaptive differentiation in this self-fertile allotetraploid grass, and use this information to develop a framework for guiding seed movement and preserving adaptive patterns of genetic variation in ongoing restoration work. Progeny of 188 families were grown for 3 years under two moisture treatments and measured for a wide range of traits involving growth, morphology, fecundity, and phenology. Variation among seed sources was analyzed in relation to physiographic and climatic trends, and to various spatial stratifications such as ecoregions, watersheds, edaphic classifications, etc. Principal component (PC) analysis extracted four primary PCs that together accounted for 67% of the variance in measured traits. Regression and cluster analyses revealed predominantly ecotypic or stepped-clinal distribution of genetic variation. Three distinct geographic groups of locations accounted for over 84% of the variation in PC-1 and PC-2 scores; group differences were best described by longitude and ecoregion. Clinal variation in PC-3 and PC-4 scores was present in the largest geographic group. Four geographic subdivisions were proposed for delimiting E. glaucus seed transfer in the Blue Mountains.Key words: Elymus glaucus, morphological variation, local adaptation, seed transfer, seed zones, polyploid.

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Agronomic performances of Pak Choi grown with different soil cover

Horticultura Brasileira ◽

10.1590/s0102-053620150000200020 ◽

2015 ◽

Vol 33 (2) ◽

pp. 261-266 ◽

Cited By ~ 1

Author(s):

Márcia M Echer ◽

Graciela M Dalastra ◽

Tiago L Hachmann ◽

Elcio S Klosowski ◽

Vandeir F Guimarães

Keyword(s):

Soil Cover ◽

Block Design ◽

Dry Weight ◽

Bare Soil ◽

Production Traits ◽

Pak Choi ◽

Randomized Block Design ◽

Black Plastic ◽

Growing Conditions ◽

Set Up

An important aspect in the cultivation of vegetables is the quality of the product to be marketed, free from dirt and damage and the practice of mulching could be an option, but there is scarse information. The aim of this study was to evaluate the influence of mulching on the production traits of three cultivars of Pak Choi. The experiment was set up during March to May 2013 using a split plot randomized block design, with four replications. Soil cover treatments (white agrotextile, black agrotextile, black plastic, silver plastic, tifton straw and bare soil) were arranged in the plots, and three cultivars of Pak Choi (Green Pak Choi, White Pak Choi and Chingensai Natsu Shomi) in subplots. Height and diameter of shoots, number of leaves, fresh weight of the head and petiole, base diameter, dry weight of stem, petiole, and leaf were evaluated thirty-five days after transplantation. The total dry weight and leaf area were measured, and then we estimated the yield. There was a significant effect of soil cover and cultivar. In general, the cover with synthetic materials showed higher values on production of Pak Choi. The cultivar White Pak Choi was better adapted to the growing conditions, with an average yield of 57.78 t/ha.

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Reproducibility of suppression of Pythium wilt of cucumber by compost

Agricultural and Food Science ◽

10.23986/afsci.41471 ◽

2014 ◽

Vol 23 (3) ◽

pp. 236-245 ◽

Cited By ~ 7

Author(s):

Mauritz Vilhelm Vestberg ◽

Sanna Kukkonen ◽

Päivi Parikka ◽

Dan Yu ◽

Martin Romantschuk

Keyword(s):

Raw Materials ◽

Dry Weight ◽

Shoot Height ◽

Bacterial Diseases ◽

Sphagnum Peat ◽

Shoot Dry Weight ◽

Suppressive Capacity ◽

The Difference ◽

Set Up

There is increasing global interest in using compost to suppress soil-borne fungal and bacterial diseases and nematodes. We studied the reproducibility of compost suppressive capacity (SC) against Pythium wilt of cucumber using nine composts produced by the same composting plant in 2008 and 2009. A bioassay was set up in a greenhouse using cucumber inoculated with two strains of Pythium. The composts were used as 20% mixtures (v:v) of a basic steam-sterilized light Sphagnum peat and sand (3:1, v:v). Shoot height was measured weekly during the 5-week experiment. At harvest, the SC was calculated as the % difference in shoot dry weight (DW) between non-inoculated and inoculated cucumbers. The SC was not affected by year of production (2008 or 2009), indicating reproducibility of SC when the raw materials and the composting method are not changed. Differences in shoot height were not as pronounced as those for shoot DW. The results were encouraging, but further studies are still needed for producing compost with guaranteed suppressiveness properties.

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The efficacy of different seed priming agents for promoting sorghum germination under salt stress

PLoS ONE ◽

10.1371/journal.pone.0245505 ◽

2021 ◽

Vol 16 (1) ◽

pp. e0245505

Author(s):

Xiaofei Chen ◽

Ruidong Zhang ◽

Yifan Xing ◽

Bing Jiang ◽

Bang Li ◽

...

Keyword(s):

Salt Stress ◽

Seed Germination ◽

Germination Rate ◽

Principal Component ◽

Dry Weight ◽

Seed Priming ◽

Nacl Stress ◽

Future Research ◽

Fresh Weight ◽

Positive Effects

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

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Evolutionary inference from QST-FST comparisons: disentangling local adaptation from altitudinal gradient selection in snapdragon plants

10.1101/385377 ◽

2018 ◽

Cited By ~ 1

Author(s):

Sara Marin ◽

Juliette Archambeau ◽

Vincent Bonhomme ◽

Mylène Lascoste ◽

Benoit Pujol

Keyword(s):

Local Adaptation ◽

Natural Populations ◽

Common Garden ◽

Global Scale ◽

Structured Populations ◽

Adaptive Divergence ◽

Adaptation To Climate Change ◽

Phenotypic Differentiation ◽

Multiple Populations ◽

Environmental Demand

ABSTRACTPhenotypic differentiation among natural populations can be explained by natural selection or by neutral processes such as drift. There are many examples in the literature where comparing the effects of these processes on multiple populations has allowed the detection of local adaptation. However, these studies rarely identify the agents of selection. Whether population adaptive divergence is caused by local features of the environment, or by the environmental demand emerging at a more global scale, for example along altitudinal gradients, is a question that remains poorly investigated. Here, we measured neutral genetic (FST) and quantitative genetic (QST) differentiation among 13 populations of snapdragon plants (Antirrhinum majus) in a common garden experiment. We found low but significant genetic differentiation at putatively neutral markers, which supports the hypothesis of either ongoing pervasive homogenisation via gene flow between diverged populations or reproductive isolation between disconnected populations. Our results also support the hypothesis of local adaptation involving phenological, morphological, reproductive and functional traits. They also showed that phenotypic differentiation increased with altitude for traits reflecting the reproduction and the phenology of plants, thereby confirming the role of such traits in their adaptation to environmental differences associated with altitude. Our approach allowed us to identify candidate traits for the adaptation to climate change in snapdragon plants. Our findings imply that environmental conditions changing with altitude, such as the climatic envelope, influenced the adaptation of multiple populations of snapdragon plants on the top of their adaptation to local environmental features. They also have implications for the study of adaptive evolution in structured populations because they highlight the need to disentangle the adaptation of plant populations to climate envelopes and altitude from the confounding effects of selective pressures acting specifically at the local scale of a population.

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Improving Mechanical Properties of Twin-Roll Cast AZ31 by Wire Rolling

Materials Science Forum ◽

10.4028/www.scientific.net/msf.1016.957 ◽

2021 ◽

Vol 1016 ◽

pp. 957-963

Author(s):

Marie Moses ◽

Madlen Ullmann ◽

Rudolf Kawalla ◽

Ulrich Prahl

Keyword(s):

Mechanical Properties ◽

Heat Treatment ◽

Rolling Texture ◽

Total Elongation ◽

Roll Casting ◽

Heat Treated ◽

Heat Treated Condition ◽

Set Up ◽

Twin Roll Cast ◽

Twin Roll

Since 2018, the institute of metal forming has been studying the novel twin-roll casting (TRC) of magnesium wire at the pilot research plant set up specifically for this purpose. Light microscopic and scanning electronic investigations were carried out within this work and show the unique microstructure of twin-roll cast AZ31 magnesium alloy with grain sizes of about 10 μm ± 4 μm in centre and 39 μm ± 26 μm near the surface of the sample. By means of a short heat treatment (460 °C/15 min), segregations can be dissolved and grain size changes in centre to 19 μm ± 12 μm (increase) and near the surface to 12 μm ± 7 μm (decrease). Further, the mechanical properties of the twin-roll cast and heat-treated wire were analysed by tensile testing at room temperature. By heat treatment, the total elongation could be increased by a third whereas the strength decreases slightly. In heat-treated state, no preferred orientation is evident. In addition to the twin-roll cast and the heat-treated condition, the rolled state was analysed. For this purpose, the twin-roll cast wire was hot rolled using an oval-square calibration. After hot rolling, a dynamic recrystallization and grain refinement of the twin-roll cast wire could be achieved. It can be seen, that an increase in strength as well as in total elongation occur after wire rolling. Beside this, a rolling texture is evident.

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Structure of the Chemical and Genetic Diversity of the True Lavender over Its Natural Range

Plants ◽

10.3390/plants9121640 ◽

2020 ◽

Vol 9 (12) ◽

pp. 1640

Author(s):

Yolande Despinasse ◽

Sandrine Moja ◽

Catherine Soler ◽

Frédéric Jullien ◽

Bernard Pasquier ◽

...

Keyword(s):

Genetic Diversity ◽

Environmental Variability ◽

Principal Component ◽

Common Garden ◽

Distribution Area ◽

Cultivated Plants ◽

Native Range ◽

Massif Central ◽

Multiple Datasets ◽

Natural Range

The true lavender Lavandula angustifolia Miller is a Mediterranean aromatic shrub widely cultivated for its high quality essential oil used in perfumery and phytotherapy. Despite its economic importance, the intra-specific diversity among wild, non-cultivated plants remains poorly understood. We analyzed the structure of the chemical and genetic diversity of plants from 14 sites sampled over the entire native range of the true lavender. Volatile organic compounds of inflorescences were analyzed using gas chromatography coupled to mass spectrometry. Genotyping was performed with fingerprinting genetic markers. To limit the influence of environmental variability on chemical composition, plants were grown in the same conditions in a common garden. Without prior knowledge, discriminant analysis of principal component identified unambiguously four distinct chemotypes among three genetic populations. Co-inertia analysis and supervised analysis which integrated multiple datasets indicated a strong congruency between chemical and genetic patterns. Two distinct genetic units were located at the edge of the distribution area in the south of Italy and in the northeast of Spain, and were associated with two distinct chemotypes. Our results confirmed the existence of three genetically distinct entities, suggesting speciation. All French populations and the Italian Piedmontese population were genetically homogeneous but separated in two distinct chemotypes. The dominant chemotype was present in the center of the native range in southeastern France and was at the origin of the current most cultivated French varieties. Its main compounds were linalyl acetate, linalool, and caryophyllene oxide. The second French chemotype was found in south of Massif Central and presented high abundance of valuable linalyl and lavandulyl acetates. Linalool, eucalyptol, β-caryophyllene, borneol, camphor, and cis-sabinene-hydrate were significantly associated with southern latitudes and their role would be worth exploring.

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The effect of molybdenum and boron in soil on the growth and photosynthesis of three soybean varieties

Plant Soil and Environment ◽

10.17221/3574-pse ◽

2011 ◽

Vol 51 (No. 5) ◽

pp. 197-205 ◽

Cited By ~ 12

Author(s):

P. Liu ◽

Y.S. Yang ◽

G.D. Xu ◽

Y.H. Shi ◽

Y.A. Yang ◽

...

Keyword(s):

Aboveground Biomass ◽

Genotypic Variation ◽

Dry Weight ◽

Photosynthesis Rate ◽

Growth Stages ◽

Pot Experiments ◽

Tolerant Plant ◽

Set Up ◽

System Volume

This paper presents a study on growth and photosynthesis response of soybean to Mo and/or B in soil. Pot experiments were set up to examine the effect of Mo and/or B on growth and photosynthesis of three soybean varieties with four treatments (control, +Mo, +B, +[Mo + B]) at various growth stages. The study showed that Mo and/or B increased main length, system volume and dry weight of the roots, aboveground biomass, leaves’ photosynthesis rate of soybean. The variation and interaction between Mo and B in soil was explored with regard to their impact on soybean growth and photosynthesis. There were some dissimilarity in growth and photosynthesis in the plants between the supplements of Mo and B in the soil, and the interrelation between Mo and B in plant and was co-supplementary to each other. Therefore, growth and photosynthesis of the soybean with Mo and B treatments were much more improved than those with Mo or B alone. Besides, some genotypic variation was found in three soybean varieties, in which Zhechun III was the most sensitive and 3811 the most tolerant plant to Mo and B.

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Allometric Equations for the Aboveground Biomass of Selected Common Eastern Hardwood Understory Species

Northern Journal of Applied Forestry ◽

10.1093/njaf/27.4.160 ◽

2010 ◽

Vol 27 (4) ◽

pp. 160-165 ◽

Cited By ~ 7

Author(s):

Yvette L. Dickinson ◽

Eric K. Zenner

Keyword(s):

Aboveground Biomass ◽

Dry Weight ◽

Vaccinium Corymbosum ◽

Allometric Equations ◽

Forest Understory ◽

Rosa Multiflora ◽

Basal Diameter ◽

Understory Species ◽

Elaeagnus Umbellata ◽

Fit Index

Abstract Allometric equations were formulated for predicting the aboveground biomass of six groups of forest understory species (Elaeagnus umbellata Thunb.], blueberry [Vaccinium angustifolium Aiton, Vaccinium corymbosum L., and Vaccinium pallidum Aiton], hawthorn [Crataegus spp.], honeysuckle [Lonicera spp.], multiflora rose [Rosa multiflora Thunb.], and viburnum [Viburnum acerifolium L. and Viburnum dentatum L.]) common to Eastern hardwoods using basal diameter and/or height. As measured by fit index, basal diameter or height alone explained between 51 and 93% of the variation in oven-dry weight; this increased to 75‐96% when both basal diameter and height were used as predictors. Data were collected at four sites throughout Pennsylvania, but an evaluation of the importance of site as a blocking factor found site not to be statistically significant; therefore, the equations presented here may be used in a variety of forested sites within the greater mid-Atlantic region.

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