scholarly journals Extract of Nanas Bongsai (Ananas comosus var. microstachys) on The Histology of The Limph and Uterus of White Rat (Rattus norvegicus)

2018 ◽  
Vol 10 (1) ◽  
pp. 223-228
Author(s):  
Fitmawati Fitmawati ◽  
Agus Saputra ◽  
Yohanes Yohanes ◽  
Hilwan Yuda Teruna ◽  
Dimas Pramita Nugraha ◽  
...  
Keyword(s):  
Side Effects ◽  
Experimental Research ◽  
Rattus Norvegicus ◽  
Ananas Comosus ◽  
White Pulp ◽  
Thin Endometrium ◽  
White Rats ◽  
Histology Result ◽  
Histopathologic Changes ◽  
Different Doses

Nanas Bongsai (Ananas comosus var. microstachys L.) is an amazing herb which is used in traditional medicine by local people of Muara Lembu district as a potion to reduce pain while menstruation period for a women. The present work was designed to investigate its probable side effects on the histopathologic changes in limph and uterus tissues after treated with A. comosus var. microstachys L extract in female white rats. This is an experimental research consisted of five treatments and three repetitions. Treatment composed of two controls (P0 and P+) and given Nanas Bongsai extract with three different dosages. Histology result of lymph show that all treatments given with Nanas Bongsai with three different dosages have different diameter average of white pulp and there is no significant changes with normal control. While observation towards histology structure of endometrium thickness with dosage P1, dosage P2, dosage P3 is 206.333±33.486; 215.667±33.486; 197.667±60.871 respectively. The most thin endometrium layer found in treatment with dosage P3 compared to control P0. From this study showed that there is no toxic effect on uterus and lymph function of of Nanas Bonsai (A. comosus var. microstachys L.) at different doses, so the plant is secure for consumption by the community.

scholarly journals Comparison of Antibacterial Effectiveness and Length of Healing Between Honey and MEBO in Degree-II Burns at the White Rat (Rattus norvegicus)

2020 ◽  
Vol 13 (1) ◽  
pp. 123-131
Author(s):  
Charles Yapiter ◽  
I Nyoman Ehrich Lister ◽  
Edy Fachrial
Keyword(s):  
Wound Healing ◽  
Experimental Research ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Antibacterial Drug ◽  
White Rats ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Second Degree

Healing Second degree burns can be affected by the presence of bacteria. This research reveals the role of honey and MEBO in removing bacterial effects on healing. Pure experimental research method with a completely randomized design (CRD). There were 4 treatments, which were positive, negative, honey and MEBO treatment, each of which was given to rats with second degree burns. Wound healing and other parameters were observed on 7 th, 14th and 21st days. The result was that the administration of honey as an antibacterial drug was very effective and gave healing on the 21st day to white rats. Giving MEBO as an antibacterial drug is very effective and provides healing on the 21st day to white rats. Giving honey is more effective than MEBO as an antibacterial drug and healing burns on 21st day against white rats.

scholarly journals EFEK PAPARAN DEKOK BIJI PINANG (Areca catechu) TERHADAP MOTILITAS SPERMATOZOA TIKUS (Rattus norvegicus): UPAYA MENEMUKAN KANDIDAT ANTIFERTILITAS PRIA (The Effect of Betel Nut Extract (Areca catechu) on Mice Spermatozoa Motility (Rattus norvegicus: Effort to Find Out Man Antifertility Candidate)

2016 ◽  
Vol 2 (2) ◽  
Author(s):  
Muslim Akmal
Keyword(s):  
Body Weight ◽  
Sperm Motility ◽  
Rattus Norvegicus ◽  
Control Group ◽  
Betel Nut ◽  
Spermatozoa Motility ◽  
White Rats ◽  
Areca Catechu ◽  
Different Doses

This research was conducted to prove the ability of betel nut extract (Areca catechu) in causingdegradation of sperm motility on rats (Rattus norvegicus) strain Wistar. This research used 2-3 monthsage, 200-250 g body weight of male white rats Rattus norvegicus strain Wistar. The rats were dividedinto 5 groups in equal number, 3 rats each group. They were a control group without treatment and 4treatment groups which were given different doses of betel nut extract for 7 days, i.e., 1, 2, 3, and 4 g/200g body weight respectively. The result showed that increasing doses of betel nut extract can result indegradation of sperm motility significantly (P0.05).Keywords: Areca catechu, Rattus norvegicus, sperm motility

scholarly journals Comparison of Antibacterial Effectiveness and Length of Healing between Honey and MEBO in Degree-II Burns at the White Rat (Rattus norvegicus)

2020 ◽  
Vol 13 (1) ◽  
pp. 164-172
Author(s):  
Charles Yapiter ◽  
I Nyoman Ehrich Lister ◽  
Edy Fachrial
Keyword(s):  
Wound Healing ◽  
Experimental Research ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Antibacterial Drug ◽  
White Rats ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Second Degree

Healing Second degree burns can be affected by the presence of bacteria. This research reveals the role of honey and MEBO in removing bacterial effects on healing. Pure experimental research method with a completely randomized design (CRD). There were 4 treatments, which were positive, negative, honey and MEBO treatment, each of which was given to rats with second degree burns. Wound healing and other parameters were observed on 7 th, 14th and 21st days. The result was that the administration of honey as an antibacterial drug was very effective and gave healing on the 21st day to white rats. Giving MEBO as an antibacterial drug is very effective and provides healing on the 21st day to white rats. Giving honey is more effective than MEBO as an antibacterial drug and healing burns on 21st day against white rats.

A new approach to treatment of stomatitis in experiments on white rats (experimental research)

2018 ◽  
pp. 168-173
Author(s):  
Е.Б. Ганина ◽  
Ю.В. Червинец ◽  
Н.В. Грудинин ◽  
В.Г. Шестакова ◽  
В.М. Червинец ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Oral Cavity ◽  
Experimental Research ◽  
Quantitative Composition ◽  
Oral Diseases ◽  
New Approach ◽  
Highly Active ◽  
White Rats ◽  
Before And After ◽  
Intact Rats

Цель исследования - охарактеризовать микробиологическую картину стоматита до и после его лечения высокоактивными культурами лактобацилл, дать оценку эффективности их применения при экспериментальном стоматите. Методика. Опыты проводились на 32 самках беспородных белых крыс массой 230 г. Моделирование стоматита включало 2 этапа: моделирование травматического стоматита и воспроизведение бактериального стоматита на базе травматического. У крыс контрольной и опытных серий на всех этапах эксперимента брали мазки с поверхности десен для характеристики микробиоценоза ротовой полости. Результаты. После обработки слизистой оболочки полости рта (СОПР) культурой патогенного штамма Staphylococcus aureus существенно снизились частота встречаемости и количество условно-патогенных микроорганизмов. Лечение стоматита у крыс культурами лактобацилл ( Lactobacillus 11 зв., Lactobacillus 2 п.рта, Lactobacillus 24 д.ст.) и их комбинацией приводило к снижению распространенности S. aureus вплоть до его исчезновения, а также к нормализации количества условно-патогенной микробиоты. Заключение. Исследования показали эффективность использования отдельных высокоактивных штаммов лактобацилл или их комбинации при лечении заболеваний СОПР, в частности бактериального стоматита. Aim. To characterize the microbiological picture of stomatitis in intact rats before and after the treatment with highly active cultured lactobacilli and to evaluate the effectiveness of this treatment in experiments on white rats. Methods. Experiments were carried out on 32 mongrel female white rats weighing 230 g. Smears were taken from the gum surface of control and experimental rats at all stages of the experiment to characterize the oral cavity microbiocenosis. Results. After treatment of the oral cavity with cultured Staphylococcus aureus , the occurrence and quantity of opportunistic microorganisms significantly decreased. The treatment of stomatitis in rats with cultured lactobacillus ( Lactobacillus 11 zv., Lactobacillus 2 p.r., Lactobacillus 24 d.st. and their combination) led to a decrease in S. aureus prevalence up to its extinction and to normalization of the quantitative composition of opportunistic microbiota. Conclusions. The study showed the effieacy of highly active lactobacillus strains individually or their combinations in the treatment of oral diseases, in particular, bacterial stomatitis.

Synergistic action of propolis with levodopa in the management of Parkinsonism in Drosophila melanogaster

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Emmanuel Tiyo Ayikobua ◽  
Josephine Kasolo ◽  
Keneth Iceland Kasozi ◽  
Ejike Daniel Eze ◽  
Abass Safiriyu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Drosophila Melanogaster ◽  
Side Effects ◽  
Motor Activity ◽  
Ethanolic Extract ◽  
Synergistic Action ◽  
Content Type ◽  
Treatment Groups ◽  
Ethanolic Extract Of Propolis ◽  
Different Doses

AbstractBackgroundThe Phosphatase and tensin-induced putative kinase 1 (PINK1B9) mutant for Drosophila melanogaster is a key tool that has been used in assessing the pathology of Parkinsonism and its possible remedy. This research was targeted toward determining the effects of ethanolic extract of propolis, with levodopa therapy in the management of Parkinsonism.MethodThe PINK1B9 flies were divided into groups and fed with the different treatment doses of ethanoic extract of propolis. The treatment groups were subjected to 21 days of administration of propolis and the levodopa at different doses after which percentage climbing index, antioxidant activity and lifespan studies were done.ResultsPropolis alone improved motor activity, antioxidant and lifespan in Drosophila melanogaster than in PINK1 flies. Propolis in combination with levodopa significantly (P<0.05) improved physiological parameters at higher than lower concentrations in Parkinsonism Drosophila melanogaster demonstrating its importance in managing side effects associated with levodopa.ConclusionPropolis is a novel candidate as an alternative and integrative medicinal option to use in the management of Parkinsonism in both animals and humans at higher concentrations.

The Effect of a Single Dose of Quazepam (Sch-16134) on the Sleep of Chronic Insomniacs

1979 ◽  
Vol 7 (6) ◽  
pp. 583-587 ◽  
Author(s):  
Thomas Roth ◽  
Elizabeth I Tietz ◽  
Milton Kramer ◽  
Mark Kaffeman
Keyword(s):  
Single Dose ◽  
Side Effects ◽  
Chronic Administration ◽  
Sleep Efficiency ◽  
Objective Measures ◽  
Subjective Measures ◽  
Sleep Maintenance ◽  
Eeg Changes ◽  
Different Doses ◽  
Polysomnographic Recording

The present study evaluated the efficacy of 25 mg of quazepam, a new benzodiazepine hypnotic, in a population of chronic insomniacs. The results indicate that a single dose (25 mg) administered for one night was efficacious when measured both objectively by polysomnographic recording and subjectively by questionnaire with no reported side-effects. The change in the objective measures paralleled the direction of change in subjective measures. Sleep efficiency and sleep maintenance were improved without EEG changes in Stages 2, 3-4, and REM. Further study is needed to evaluate the effects of chronic administration of different doses of quazepam in chronic insomniacs.

scholarly journals Morphometric and Histopathologic Analysis of Lymphoid Depletion in Murine Spleens Following Infection with Brucella abortus strains 2308 or RB51 or an htrA Deletion Mutant

1996 ◽  
Vol 33 (3) ◽  
pp. 282-289 ◽  
Author(s):  
M. V. Palmer ◽  
N. F. Cheville ◽  
F. M. Tatum
Keyword(s):  
Morphometric Analysis ◽  
Brucella Abortus ◽  
Marginal Zone ◽  
White Pulp ◽  
Splenic White Pulp ◽  
Lymphoid Depletion ◽  
Histopathologic Changes ◽  
Histopathologic Analysis ◽  
Marginal Zones

BALB/C mice were inoculated intraperitoneally with suspensions of Brucella abortus strains 2308 or RB51 or an htrA mutant. Spleens were examined on postinoculation day (PID) 2, 4, 7, 10, 15, 21, 30, and 60. Brucellae were cultured in high numbers from the spleens of mice infected with strains 2308 or htrA through PID 60; however, mice infected with strain RB51 cleared the infection between PID 30 and PID 60. Histopathologic changes in spleens from 2308-infected mice were characterized by marked accumulations of macrophages, which expanded marginal zones beginning as early as PID 7 and persisting through PID 60. Morphometric analysis showed a decrease in splenic white pulp in 2308-infected mice at PID 10, which correlated with the peak of bacterial infection. Although this decrease was significant ( P < 0.05) when compared with values at the previous (PID 7) and the following (PID 15) time periods, it was not significantly different from white pulp values noted at PID 2 or PID 4 or the values for control spleens. Spleens from RB51-infected mice showed only mild to moderate accumulations of macrophages in marginal zone areas during the peak of RB51 infection (PID 7-10). Morphometric analysis of RB51-infected spleens showed a decrease in white pulp area, which coincided with peak bacterial numbers. However, this decrease was not significant ( P > 0.05). Spleens from mice infected with the htrA mutant showed moderate to marked accumulations of macrophages in marginal zone areas, which persisted through PID 60. Multifocal necrosis in lymphoid follicles as early as PID 4 was seen in both htrA and 2308 infection. Morphometric analysis of htrA-infected spleens revealed no significant decrease in white pulp and no obvious correlation with bacterial numbers in the spleen. These results suggest that virulent B. abortus does not induce lymphoid depletion significantly below those values seen in noninfected mice; thus, the possible role of lymphoid depletion in the pathogenesis of brucellosis remains questionable.

scholarly journals THE EFFECT OF NONI (Morinda citrifolia) EXTRACT TO HEPAR HISTOPATHOLOGY AND TRIGLYCERIDE LEVEL IN RATS (Rattus Norvegicus) INDUCED BY DISLIPIDEMIA

2019 ◽  
Vol 20 (2) ◽  
pp. 76-81
Author(s):  
Jhouharotul Faradisah ◽  
Diah Purwaningsari
Keyword(s):  
Lipid Metabolism ◽  
Triglyceride Level ◽  
Rattus Norvegicus ◽  
Morinda Citrifolia ◽  
Control Group ◽  
High Cholesterol Diet ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
White Rats ◽  
Abnormal Lipid Metabolism

Dyslipidemia is an abnormal lipid metabolism which may cause fat degeneration on hepatocytes cells and elevated triglyceride serum level. Dyslipidemia can be prevented by the consumption of high antioxidant food. Noni(Morinda citrifolia) contains many antioxidant such as flavanoid, kuersetin, tannin, and saponin, which are able to prohibit the elevation of ROS.This research is aimed to find out the effect of noni(Morinda citrifolia) extract in reducing the number of hepatocyte’s cells with fat degeneration and decreasing the triglyceride level which is elevated due to high cholesterol diet induction.In this study white rats divided randomly into 4 groups, control group (K-), high cholesterol diet induced group (K+), high cholesterol diet induced with 100 mg/Kg BW noni extract group (P1), high cholesterol diet induced with 200 mg/Kg BW noni extract group (P2). The result shows that noni  extract with dose 100 mg/Kg BW and  200 mg/Kg BWcan reduce the number of hepatocytes cells with fat degeneration (p= 0,026 and p=0,027) and decrease the level of triglyceride serum (p=0,036 and p=0,010).The conclusion is noni extract with dose 100 mg/KgBW reduces effectively  the number of hepatocyte’s cells with fat degeneration and decreases the level of triglyceride serum which increase because of high cholesterol diet. 

scholarly journals PENGARUH PEMBERIAN INFUSA DAUN LABU SIAM (Sechium edule) TERHADAP PENURUNAN KADAR KOLETEROL DARAH TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 151
Author(s):  
Ausich Singal ◽  
Edwin De Queljoe ◽  
Paulina Yamlean
Keyword(s):  
Rattus Norvegicus ◽  
Dose Group ◽  
T Test ◽  
Blood Cholesterol ◽  
Control Group ◽  
Cholesterol Levels ◽  
White Rats ◽  
Sechium Edule ◽  
One Way Anova ◽  
Paired T Test

ABSTRACT This study aims to determine the effect of infusion of conjoined pumpkin leaves (Sechium edule) on reducing total blood cholesterol of male white rats (Rattus norvegicus). The subjects of this study were 15 male white rats with an average body weight of 200 grams which were divided into 5 groups, each group consisted of 3 mice. The method used is a laboratory experiment with a completely randomized design. The results were obtained from 2 measurements of blood cholesterol levels, namely measurements before and after treatment. The treatment begins with the provision of high-fat foods for 48 days. On the 49th day a blood cholesterol level was measured before treatment. Furthermore, treatment was given to each group, namely aquades in the negative control group, simvastatin in the positive control group, and squash leaves infusion with their respective doses in the dose group I (40%), the dose group II (20%), and the dose group III (10%). Measurement of cholesterol levels after treatment was carried out on day 54. Data were analyzed by Paired t-test and One Way ANOVA. The analysis showed that there were no significant differences between treatment groups. Judging from the change in average and percentage, 40% infusion dose of siamese pumpkin leaves gives the best reduction in cholesterol levels. Keywords: Cholesterol, pumpkin leaves, male white mouse infusion. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian infusa daun labu siam (Sechium edule ) terhadap penuruan kolesterol darah total tikus putih jantan (Rattus norvegicus). Subjek penelitian ini berupa tikus putih jantan berjumlah 15 ekor dengan berat badan rata-rata 200 gram yang dibagi dalam 5 kelompok, masing-masing kelompok sebanyak 3 ekor. Metode yang digunakan yaitu eksperimen laboratorium dengan rancangan acak lengkap. Hasil penelitian diperoleh dari 2 kali pengukuran kadar koleterol darah yaitu pengukuran sebelum dan sesudah perlakuan. Perlakuan dimulai dengan pemberian makanan tinggi lemak selama 48 hari. Pada hari ke49 dilakukan pengukuran kadar kolesterol darah sebelum perlakuan. Selanjutnya diberikan perlakuan pada tiap kelompok yaitu aquades pada kelompok kontrol negatif, simvastatin pada kelompok kontrol positif, dan infusa daun labu siam dengan dosis masing-masing pada kelompok dosis I (40 %), kelompok dosis II (20 %), dan kelompok dosis III (10%). Pengukuran kadar kolesterol sesudah perlakuan dilakukan pada hari 54. Data diananlisis dengan Paired t-test dan One Way ANOVA. Hasil analisa menunjukan tidak terdapat perbedaan yang bermakna antar kelompok perlakuan. Dilihat dari perubahan rerataan dan presentase, dosis infusa  40% daun labu siam memberikan penurunan kadar koleterol terbaik. Kata kunci : Infusa daun Labu siam, kolesterol, tikus putih jantan.

scholarly journals UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 160
Author(s):  
Adinda Fransisca Pongoh ◽  
Edwin De Queljoe ◽  
Henki Rotinsulu
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Carica Papaya ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Control Group ◽  
Homogeneity Test ◽  
Glucose Levels ◽  
White Rats ◽  
Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan.  ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.)  dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

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