scholarly journals Comparison of Antibacterial Effectiveness and Length of Healing between Honey and MEBO in Degree-II Burns at the White Rat (Rattus norvegicus)

2020 ◽  
Vol 13 (1) ◽  
pp. 164-172
Author(s):  
Charles Yapiter ◽  
I Nyoman Ehrich Lister ◽  
Edy Fachrial
Keyword(s):  
Wound Healing ◽  
Experimental Research ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Antibacterial Drug ◽  
White Rats ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Second Degree

Healing Second degree burns can be affected by the presence of bacteria. This research reveals the role of honey and MEBO in removing bacterial effects on healing. Pure experimental research method with a completely randomized design (CRD). There were 4 treatments, which were positive, negative, honey and MEBO treatment, each of which was given to rats with second degree burns. Wound healing and other parameters were observed on 7 th, 14th and 21st days. The result was that the administration of honey as an antibacterial drug was very effective and gave healing on the 21st day to white rats. Giving MEBO as an antibacterial drug is very effective and provides healing on the 21st day to white rats. Giving honey is more effective than MEBO as an antibacterial drug and healing burns on 21st day against white rats.

scholarly journals Comparison of Antibacterial Effectiveness and Length of Healing Between Honey and MEBO in Degree-II Burns at the White Rat (Rattus norvegicus)

2020 ◽  
Vol 13 (1) ◽  
pp. 123-131
Author(s):  
Charles Yapiter ◽  
I Nyoman Ehrich Lister ◽  
Edy Fachrial
Keyword(s):  
Wound Healing ◽  
Experimental Research ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Antibacterial Drug ◽  
White Rats ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Second Degree

Healing Second degree burns can be affected by the presence of bacteria. This research reveals the role of honey and MEBO in removing bacterial effects on healing. Pure experimental research method with a completely randomized design (CRD). There were 4 treatments, which were positive, negative, honey and MEBO treatment, each of which was given to rats with second degree burns. Wound healing and other parameters were observed on 7 th, 14th and 21st days. The result was that the administration of honey as an antibacterial drug was very effective and gave healing on the 21st day to white rats. Giving MEBO as an antibacterial drug is very effective and provides healing on the 21st day to white rats. Giving honey is more effective than MEBO as an antibacterial drug and healing burns on 21st day against white rats.

scholarly journals Effect of Iles-Iles (Amorphophallus oncophyllus) Synbiotic Effervescent Tablet to Decrease in Blood Sugar Levels in Hyperglycemic White Rat (Rattus norvegicus)

2020 ◽  
Vol 2 ◽  
pp. 00004
Author(s):  
Ngatirah Ngatirah ◽  
Meidi Syaflan ◽  
Satria A. N
Keyword(s):  
Blood Sugar ◽  
Rattus Norvegicus ◽  
Normal Dose ◽  
White Rats ◽  
Randomized Design ◽  
Treatment Dose ◽  
Completely Randomized Design ◽  
Sugar Levels ◽  
And Control ◽  
Effervescent Tablet

This study aims to determine the effect of giving Iles-iles synbiotic effervescent tablets to decrease blood sugar levels of hyperglycemic white rats and find out the lowest dose of effervescent tablets in decreasing blood sugar levels. This study used the Completely Randomized Design (CRD) with single factor (dose of Iles-iles synbiotic effervescent tablet) with 4 levels (dose 0.045 gram/day (½ normal dose), dose 0.09 gram/day (normal dose), dose 0.18 gram/day (2X normal dose) and Control (without treatment dose). The analysis carried out was blood sugar levels and rat body weight. The administration of Iles-iles synbiotic effervescent tablets can reduce blood sugar levels in white mice but does not affect changes in the weight of white mice. The biggest decrease in blood sugar levels in white rats for synbiotic month was obtained from the administration of a dose of 0.18 grams/day (normal 2X dose) which was as much as 49.16% The administration of effervescent tablets in the fourth week with a normal dose of 0.09 grams/200 grams of weight has been able to reduce blood sugar levels in white rats to normal levels. 

scholarly journals Pengaruh penambahan media limbah jamur merang dalam pupuk organik cair terhadap konsentrasi kaslium

2020 ◽  
Vol 1 (1) ◽  
pp. 28-36
Author(s):  
Yuli Anggraini Putri ◽  
Hulyadi Hulyadi ◽  
Dahlia Rosma Indah
Keyword(s):  
Organic Matter ◽  
Experimental Research ◽  
Research Method ◽  
Organic Fertilizer ◽  
Potassium Content ◽  
Randomized Design ◽  
Completely Randomized Design

Pupuk organic cair merupakan pupuk yang berupa larutan dari hasil pembusukan bahan-bahan organik. Kombinasi whey tahu dan limbah jamur merang dengan penambahan starter mikroorganisme lokal (MOL) dapat diolah menjadi pupuk organik cair mengandung makro nutrient Kalium (K-total). Tujuan penelitian ini untuk mengetahui kandungan kalium optimum pada pupuk organik cair whey tahu dengan penambahan limbah jamur merang dan mikroorganisme lokal (MOL). Metode penelitian ini menggunakan penelitian eksperimen di laboratorium dengan menggunakan metode Rancangan Acak Lengkap (RAL) dengan dua: faktor 1 massa limbah jamur merang (Limbah jamur merang lama 600 gr dan limbah jamur merang baru 600 gr) dan faktor 2 volume pupuk organik cair (masing-masing 1L). Hasil penelitian menunjukkan bahwa kandungan K-total tinggi terdapat pada perlakuan POC + LB yaitu 1,05%.The effect of the addition of mushroom waste media in liquid organic fertilizer to the concentration of cassava AbstractLiquid organic fertilizer is a fertilizer in the form of a solution from the decay of organic matter. The combination of whey tofu and mushroom waste with the addition of local microorganism starter (MOL) can be processed into liquid organic fertilizer containing macro-nutrient potassium (K-total). The purpose of this study was to determine the optimum potassium content in the liquid organic fertilizer of whey tofu with the addition of mushroom waste and local microorganisms (MOL). This research method used experimental research in the laboratory using the Completely Randomized Design (CRD) method with two: a factor of 1 mass of mushroom waste (600 gr old mushroom waste and 600 gr new mushroom waste) and a factor of 2 volume of liquid organic fertilizer (each -Each 1L). The results showed that the high total K content found in the POC + LB treatment was 1.05%.

scholarly journals Pengaruh Pemberian Teh Kombucha Konsentrasi 50% dengan Waktu Fermentasi yang Berbeda pada Struktur Histologi Hepar Tikus Wistar (Rattus norvegicus) Jantan

2021 ◽  
Vol 23 (1) ◽  
pp. 51-56
Author(s):  
Sri Isdadiyanto ◽  
Silvana Tana
Keyword(s):  
Data Analysis ◽  
Rattus Norvegicus ◽  
Cell Damage ◽  
Control Treatment ◽  
Histological Structure ◽  
White Rats ◽  
Randomized Design ◽  
Kombucha Tea ◽  
Significant Difference ◽  
Completely Randomized Design

This study was conducted to determine the effect of 6 kombucha fermented tea (P1), 9 (P2) and 12 (P3) fermentation days at a concentration of 50% on the liver histological structure and hepatocyte diameter. This study used 16 male white rats consisting of 4 treatments and 4 replications. The treatment of this study consisted of P0: control treatment; P1, P2 and P3 respectively were given 6, 9 and 12 days fermented kombucha tea at a concentration of 50%. This research uses a completely randomized design. The variable measured in this study was the diameter of hepatocytes in test animals. The data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level and if there were real differences, followed by Duncan's test. The results of data analysis showed that kombucha tea fermented 6, 9 and 12 days with a 50% concentration did not have a significant difference in hepatocyte diameter variables. The histological structure of the liver looks normal and shows no sign of cell damage. It can be concluded that kombucha tea at a concentration of 50% is safe for consumption by mammals because it does not cause liver damage.

scholarly journals UJI AKTIVITAS ANTIHIPERURISEMIA KOMBINASI EKSTRAK ETANOL DAUN SALAM (Syzygium polyanthum (Wight.) Walp) dan TUMBUHAN SURUHAN (Peperomia pellucida (L.) Kunth) PADA TIKUS PUTIH JANTAN (Rattus Norvegicus)

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 581
Author(s):  
Chintia M. Manopo ◽  
Widdhi Bodhi ◽  
Elly J. Suoth
Keyword(s):  
Rattus Norvegicus ◽  
Design Method ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
White Rats ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Ethanol Extracts ◽  
Existing Data

ABSTRACTBay leaves (Syzygium polyanthum (Wight.) Walp) and herbs (Peperomia pellucida (L.) Kunth) are known to contain flavonoids, where flavonoids have been known to have the ability to inhibit the ksantin oxidase, which causes uric acid levels in the blood to drop. This study used a completely randomized design method. This study aims to determine whether the combined ethanol extract of bay leaves (Syzygium polyanthum (Wight.) Walp) and suruhan (Peperomia pellucida (L.) Kunth) has antihyperuricemic activity. There were 15 rats used and divided into 5 groups, namely negative control (CMC 1%), positive control (Allopurinol), and ethanol extracts of bay leaves and herbs with a dose of 50 m, 100 mg, 200 mg. Existing data were analyzed using the ANOVA test and LSD test. The analysis showed that the combined ethanol extract of bay leaves (Syzygium polyanthum (Wight.) Walp) and suruhan (Peperomia pellucida (L.) Kunth) had antihyperuricemic activity against male white rats. Keywords: Syzygium polyanthum (Wight.) Walp) and Peperomia pellucida (L.) Kunth, Antihyperuricemia, Rattus Norvegicus.ABSTRAKDaun salam (Syzygium polyanthum (Wight.) Walp) dan tumbuhan suruhan (Peperomia pellucida (L.) Kunth) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan dalam menghambat ksantin oksidase, yang menyebabkan kadar asam urat di dalam darah turun. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol kombinasi dari daun salam (Syzygium polyanthum (Wight.) Walp ) dan tumbuhan suruhan (Peperomia pellucida (L.) Kunth) memiliki aktivitas antihiperurisemia. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (CMC 1%), kontrol positif (Allopurinol), dan ekstrak etanol daun salam dan tumbuhan suruhan dengan dosis 50 mg, 100 mg, 200 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menunjukkan bahwa ekstrak etanol kombinasi daun salam (Syzygium polyanthum (Wight.) Walp) dan tumbuhan suruhan (Peperomia pellucida (L.) Kunth ) memiliki aktivitas antihiperurisemia terhadap tikus putih jantan.Kata kunci: Syzygium polyanthum (Wight.) Walp) dan  Peperomia pellucida (L.) Kunth, Antihiperurisemia, Rattus Norvegicus

Pengaruh Pemberian Moringa oleifera Terhadap Kuantitas dan Kualitas Semen Sapi Peranakan Ongole

2020 ◽  
Vol 7 (2) ◽  
pp. 119
Author(s):  
Lukman Affandhy ◽  
Muchamad Luthfi ◽  
Dian Ratnawati ◽  
Frediansyah Firdaus
Keyword(s):  
Data Analysis ◽  
Analysis Of Variance ◽  
Research Method ◽  
Moringa Oleifera ◽  
The Third ◽  
Randomized Design ◽  
Leaf Powder ◽  
Completely Randomized Design ◽  
Second Period

ABSTRAK  Penelitian bertujuan untuk mengetahui pengaruh pemberian bubuk daun Moringa oleifera (MO) terhadap kuantitas dan kualitas semen sapi peranakan ongole (PO). Metode penelitian menggunakan percobaan lapang dengan rancangan acak lengkap dengan tiga perlakuan pemberian MO sebesar 0 kg/ekor/hari (P1); 0,05 kg/ekor/hari (P2) dan 0,1 kg/ekor/hari (P3). Analsis data menggunakan one way analysis of variance. Masing-masing perlakuan terdiri atas empat ekor dengan tiga periode pengamatan sebagai ulangan, yaitu periode pertama dan ketiga tanpa MO, dan periode kedua diberikan MO pada P2 dan P3. Hasil penelitian menunjukan bahwa volume dan pH semen perlakuan P1, P2 dan P3 tidak berbeda semua periode pengamatan. Motilitas massa spermatozoa P2 dan P3 periode kedua dan ketiga menunjukan nilai +++ berbeda nyata (P<0,05) dibandingkan dengan P1 (++); motilitas individu  perlakuan P1 menunjukkan nilai terendah 60,56±2,94 % (P<0,05) dibandingkan P2 dan P3 pada periode kedua dan ketiga (75,50±2,29 dan 72,50±2,34%). Konsentrasi spermatozoa P2 dan P3 periode kedua dan ketiga menunjukkan rata-rata  >1.350 juta/ml berbeda nyata (P<0,05) daripada P1 (876±152juta/ml), sedangkan total spermatozoa motil menunjukkan nilai tertinggi 5.647±829 juta/ml pada P3 periode kedua.Viabilitas spermatozoa P2 dan P3 menunjukkan nilai >88-89 % beda nyata (P<0,05) daripada P1 (<84 %) pada periode kedua dan ketiga, sedangkan nilai abnormalitas spermatozoa yang terbaik adalah P2 sebesar 4,30% pada periode kedua dan P3 sebesar 5,33% pada periode ketiga. Disimpulkan pemberian bubuk daun MO dengan dosis 0,1 kg/ekor/hari dapat memperbaiki kuantitas dan kualitas semen (viabilitas dan total spermatozoa motil) sapi pejantan PO.Kata kunci:  kuantitas dan kualitas semen, Moringa oleifera, sapi pejantan ABSTRACTThe research aimed to determine the effect of Moringa oleifera (MO) leaf powder on the quantity and quality of semen of ongole crossbreed. The research method used was a field experiment with a completely randomized design with three treatments giving MO of 0 kg/head/day (P1); 0.05 kg/head/day (P2) and 0.1 kg/head/day (P3). Each treatment consisted of four heads with three periods of observation as replications, namely the first and third periods without MO, and the second period was given MO on P2 and P3. Data analysis used a one-way analysis of variance. The results showed that the volume and pH of semen treated P1, P2, and P3 didn’t different in all observation periods. The second and third period spermatozoa mass motility of P2 and P3 showed that the value of +++ was significantly different (P<0.05) compared to P1 (++); Individual motility of P1 treatment showed the lowest value 60,56±2,94 % (P<0.05) compared to P2 and P3 in the second and third periods (75,50±2,29 and 72,50±2,34%). The second and third periods of P2 and P3 spermatozoa concentrations showed an average of >1,350 million/cc significantly different (P<0.05) than P1 (876±152 million/cc), while the total motile spermatozoa showed the highest value of 5,647±829 million/cc in the second period P3. The spermatozoa viability of P2 and P3 showed values >88-89% significantly different (P<0.05) than P1 (<84%) in the second and third periods, while the best spermatozoa abnormalities were P2 at 4.30% in the second period and P3 at 5.33% in the third period. It was concluded that the giving of MO leaf powder at a dose of 0,1 kg/head/day could improve the quantity and quality of semen (viability and total motile spermatozoa) of Ongole Crossbreed bulls. Keywords: bulls, Moringa oleifera, quantity and quality of semen

Analysis of Fibronectin and TGF-β1 Immunoexpression to Determination of Wound Vitality in Wistar Rats (Rattus norvegicus)

2015 ◽  
Vol 1 (1) ◽  
Author(s):  
Nita Novita ◽  
Hasrayati Agustina ◽  
Bethy S. Hernowo ◽  
Abdul H. Hassan
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Age Determination ◽  
Scientific Field ◽  
Wound Age ◽  
Forensic Practice ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Tgf Β1

Wound examination is indispensable in forensic practice. The scientific field of wound age determination has advanced progressively during recent years.The purpose of this study was to determine the differences of fibronectin and TGF-β1 expression in both antemortem and postmortem wounds. This study was an experimental with completely randomized design.  The skin wounds (vital and postmortem) were taken from fourty Wistar rats and divided into 10 groups of rats. Immunohistochemical staining was performed to determine the differences between antemortem and postmortem wounds. The result showed that in 30 minutes after antemortem wound infliction, all of samples showed weak reactivity for fibronectin and TGF-β1 (100%).  In first hour after wound infliction, 3 samples (75%) showed weakly positive and 1 sample (25%) strongly positive for fibronectin and TGF-β1.  In 2 hour after wound infliction, 1 sample (25%) showed weakly positive and 3 sample (75%) strongly positive for fibronectin and TGF-β1.  In 3 and 4 hour after wound infliction, all of samples strongly positive for fibronectin and TGF-β1.  In postmortem wound, all of samples showed negativity for fibronectin and TGF-β1. In conclusion, fibronectin and TGF-β1 may be useful in the determination of wound vitality. Keywords: wound, fibronectin, TGF-β1, vitality

scholarly journals Microplastic Depuration on Asaphis Detlorata

2020 ◽  
Vol 1 (2) ◽  
pp. 37-46
Author(s):  
Dian Fatriani Indah Saputri ◽  
Anwar Daud ◽  
Rachman Syah ◽  
Agus Bintara Birawida ◽  
Hasnawati Amqam ◽  
...  
Keyword(s):  
Research Design ◽  
Analysis Of Variance ◽  
Experimental Animal ◽  
Experimental Research ◽  
River Estuary ◽  
Circulation System ◽  
Randomized Design ◽  
Water Circulation System ◽  
Completely Randomized Design ◽  
Post Hoc

Depuration is an effort to reduce/eliminate contamination including microplastics, which one is using a water circulation system. This study aims to determine the effective depuration time to reduce the microplastic content in Asaphis detlorata This study used a quantitative approach with experimental research design with a completely randomized design. There are 450 shells used as an experimental animal where is the treatment consisted of four depuration times, namely 1;2;3; and 4 days with 3 repetitions of each treatment, while the control shells were without depuration. Analysis of variance (ANOVA) was used to see the effect of depuration treatment on the microplastic content. If the effect of the treatment was significantly different, then the post hoc test was continued to determine the differences between treatments. The results showed that Asaphis detlorata obtained from the mouth of the Lakatong river estuary were contaminated with microplastics ranging from 0.6 to 8.1 MPs/shellfish and an average of 3.96 MPs/shellfish. Depuration time significantly affected the microplastic content in shellfish depuration effectiveness. There is a tendency that the longer depuration time is decreased microplastic content in shellfish. The effective depuration time to reduce the microplastic content in Asaphis detlorata was 3 and 4 days. Further research is needed for a more effective depuration for cleaning microplastics in shellfish.

scholarly journals The Role of MycoSilvi, Lime and Compost on The Growth of Balsa (Ochroma bicolor Rowlee.) Seedling in Post Silica Sand Mine Media

2020 ◽  
Vol 3 (01) ◽  
pp. 28-39
Author(s):  
Sri Wilarso ◽  
Putri Maharani ◽  
Andi Sukendro ◽  
Cahyo Wibowo
Keyword(s):  
Glomus Mosseae ◽  
Silica Sand ◽  
Lime Treatment ◽  
Mycorrhizal Dependency ◽  
Randomized Design ◽  
Total Growth ◽  
Completely Randomized Design ◽  
Plot Design

Problems arising from silica sand mining are decreasing soil fertility, as well as the presence of high heavy metals such as Fe and Al, which inhibits the growth of revegetation plants. The purpose of this study was to analyze the response of the growth of balsa seedlings (Ochroma bicolor Rowlee.) on silica sand post-mining media treated with MycoSilvi, compost, and lime and determine the most optimal combination of MycoSilvi, compost, and lime treatment. This study uses a completely randomized design (CRD) with a split-plot design with 3 treatment factors, namely the addition of MycoSilvi, compost, and lime. Each treatment consisted of five replications. The results showed that the planting media that were not treated (control) was not able to support the growth of balsa seedlings. The interaction of MycoSilvi, compost, and lime significantly affected the height, diameter, biomass, and percentage of mycorrhizal colonization parameters, and significantly affected the plant chlorophyll content. The combination of MycoSilvi type 1 treatment, namely Glomus mosseae and lime (C0K1M1) type, resulted in the highest total growth rate, diameter, biomass, and colonization percentage compared to other treatments. The addition of lime and compost to the growth medium can reduce the degree of mycorrhizal dependency of balsa seedling.

scholarly journals UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa
Keyword(s):  
Rattus Norvegicus ◽  
Design Method ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Randomized Design ◽  
Decrease Blood Glucose ◽  
Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

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