Characteristics of reproductive parameters and quality of sexual products of female carp (Cyprinus carpio (Linnaeus, 1758)) when feeding them with hulless oat during prespawning period

Purpose. To investigate the effect of feeding carp with hulless oat during pre-spawning period on the reproductive functions of female carp as well as on qualitative characteristics of the obtained eggs. Methodology. The objects of the study were females of the Nyvky intra-breed type of the Ukrainian scaly carp breed, which were kept during the pre-spawning period in pond conditions of the State Enterprise “Nyvka” of the Institute of Fisheries NAAS of Ukraine. Females were divided into 3 groups. The first group (Experiment I) was fed with 30% hulless oat, the second (Experiment II) with 100% hulless oat. The control group of fish (Control) was fed with a grain mixture. Experiments were conducted in accordance with current methods used in aquaculture. The mass fraction of protein was determined by the Kjeldahl method, the fat content was determined by S.V. Rushkovsky method. Determination of total lipids was performed by the Folch method. Sampling of water for chemical analysis and its processing in the laboratory was carried out according to O.A. Alokin method according to the Standard of the Ministry of Agrarian Policy of Ukraine. Analytical processing of the material was performed using MS Excel. The criteria for the analysis of studied parameters were their mean values (M) and standard deviations (m). Results. According to studies, the use of 30% hulless oat in the feed of female carp during the pre-spawning has a positive effect on their production of eggs, as evidenced by an increase in relative fertility by 15.6%, fertilization rate by 4.0%, and survival of the obtained 3-day-old larvae by 5.0%. According to biochemical parameters of eggs, there was an increase in the amount of essential amino acids by 3.04 and 2.01 g/100 g of protein, and fractions of lipids responsible for energy storage, in particular triglycerides, by 11.56 and 5.62%, phospholipids by 3.65 and 2.37%, and fatty acids by 5.33 and 3.11%, respectively, according to the experimental groups. Instead, cholesterol levels decreased by 13.4 and 25.2%. Temperature, oxygen, and hydrochemical regimes in the hatchery were within aquaculture standards, except for permanganate oxidation and total iron and chlorine ions. Originality. The prospects of using hulless oat in feeding brood carp were studied in Ukraine for the first time. The analysis of reproductive parameters of female carp as a result of experimental feeding and evaluation of the qualitative characteristics of eggs and offspring obtained by the method of factory reproduction was conducted. Practical value. The results of a comprehensive assessment of aquaculture and biological parameters will provide recommendations for the use of hulless oat in feeding female carp during pre-spawning period. Key words: carp, feeding, hulless oats, reproductive parameters, survival, amino acid and lipid composition of eggs.

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Characteristics of biochemical indicators of sperm production of carp males while using naked oats in the nutrition process during pre-sprawning period

Animal Science and Food Technology ◽

10.31548/animal2021.02.011 ◽

2021 ◽

Vol 12 (2) ◽

Author(s):

N Syrovatka ◽

◽

I Hrytsyniak ◽

D Syrovatka ◽

I Yaremchuk ◽

...

Keyword(s):

State Enterprise ◽

Control Group ◽

Rectilinear Motion ◽

Sperm Production ◽

Spawning Period ◽

Experimental Fish ◽

Fish Sperm ◽

Activity Of Enzymes ◽

Naked Oats

In the research there have been used males of the Nyvka intra-breed type of the Ukrainian scaly carp breed, which were kept during the pre-spawning period in the pond conditions of the State Enterprise “Experimental fish farm Nyvka” within the Institute of Fisheries of the National Academy of Agrarian Sciences of Ukraine (IFNAASU). The carps were divided into 3 groups. The first group (Experiment I) was fed 100 % naked oats, the second (Experiment II) – 30 %, the control group of fish (Control) was fed a grain mixture. The quality of sexual products of male carp was assessed by determining the effect of adding naked oats to their diet during the pre-spawning period. Indicators of sperm motility and fertilizing ability, content of γ-, β- and α-globulins, albumin, prealbumin, and physiological characteristics of ejaculates of respiratory and regenerative capacity, activity of enzymes of respiratory chain of mitochondria (CHO and SDG) were studied. Given the increased productivity of fish sperm, in particular the increase in the number of sperm with rectilinear motion, decrease in the number of inanimate sperm and changes in the protein formula of germ cells, it is advisable to add naked oats to the main diet in the amount of 30 %.

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Protective Effect of Moringa Oleifera Leaves Against TramadolInduced Nephrotoxicity in Mice

INTERNATIONAL JOURNAL OF TOXICOLOGICAL AND PHARMACOLOGICAL RESEARCH ◽

10.25258/ijtpr.v9i02.9053 ◽

2017 ◽

Vol 9 (02) ◽

Author(s):

Ashraf Albrakati

Keyword(s):

Oral Dose ◽

Moringa Oleifera ◽

Treated Group ◽

Control Group ◽

Serum Urea ◽

Kidney Function Tests ◽

Mean Values ◽

Intraperitoneal Dose ◽

Moringa Oleifera Leaves ◽

The Mean

Tramadol, a broadly in recent years, is an effective analgesic agent for the treatment of moderate to acute pain. Its metabolites are excreted by the kidney which may cause nephrotoxicity. Moringa oleifera leaves are commonly used to provide herbal and plant-derived medicinal products especially in developing nations. The present study was carried out to determine the biochemical and histopathological changes in the kidney of tramadol-treated albino mice and to evaluate the possible protective role of Moringa oleifera leaves against tramadol-induced nephrotoxicity. Twenty adult albino mice were divided into four groups. Control group (group i) received daily intraperitoneal injection of normal saline only, group ii received oral dose of Moringa oleifera leaves extract (20 mg/kg/bw) for three weeks, group iii received daily intraperitoneal dose of tramadol (0.3 mg/kg/bw) for the same period, group iv, received daily oral dose of Moringa oleifera leaves extract, (20 mg/kg/bw) three hours before injecting intraperitoneal dose of tramadol (0.3 mg/kg/bw), for the same period. Blood samples were withdrawn at the end of the experiment for kidney function tests and specimens from the kidney were processed for histological study. No significant differences in the mean values of the kidney function tests were noticed between Moringa oleifera group and control group. However, there was highly significant increase in the mean values of serum, urea and creatinine in tramadol-treated group as compared to the control group. Although tramadol + Moringa oleifera group revealed significant difference in the mean values of urea and creatinine when compared with tramadol-treated group. So, Moringa oleifera leaves extract have been shown to attenuate the renal dysfunction, improve the renal architecture, with nearly normalization of serum urea and creatinine levels which indicate improvement of renal function. In conclusion, in the light of biochemical results and histological findings, co-administration of Moringa oleifera leaves lessened the negative effects of tramadol-induced nephrotoxicity; possibly by its antioxidant action. Further investigation of these promising protective effects of Moringa oleifera leaves against tramadol-induced renal injury may have considerable impact on developing an adjunct therapy aiming to improve the therapeutic index of some nephrotoxic drugs.

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Changes in the Composition and Function of Lipoproteins after Bariatric Surgery in Patients with Severe Obesity

Journal of Clinical Medicine ◽

10.3390/jcm10081716 ◽

2021 ◽

Vol 10 (8) ◽

pp. 1716

Author(s):

Idoia Genua ◽

Núria Puig ◽

Inka Miñambres ◽

Sonia Benítez ◽

Pedro Gil ◽

...

Keyword(s):

Bariatric Surgery ◽

Lipid Profile ◽

Healthy Subjects ◽

Control Group ◽

Morbidly Obese ◽

Qualitative Properties ◽

Ldl Particles ◽

Obese Subjects ◽

Lipoprotein Composition ◽

Qualitative Characteristics

The effect of bariatric surgery on lipid profile and the qualitative characteristics of lipoproteins was analyzed in morbidly obese subjects. Thirteen obese patients underwent bariatric surgery. Plasma samples were obtained before surgery and at 6 and 12 months after the intervention. Thirteen healthy subjects comprised the control group. Lipid profile, hsCRP, and the composition and functional characteristics of VLDL, LDL, and HDL were assessed. At baseline, plasma from subjects with obesity had more triglycerides, VLDLc, and hsCRP, and less HDLc than the control group. These levels progressively normalized after surgery, although triglyceride and hsCRP levels remained higher than those in the controls. The main differences in lipoprotein composition between the obese subjects and the controls were increased apoE in VLDL, and decreased cholesterol and apoJ and increased apoC-III content in HDL. The pro-/anti-atherogenic properties of LDL and HDL were altered in the subjects with obesity at baseline compared with the controls, presenting smaller LDL particles that are more susceptible to modification and smaller HDL particles with decreased antioxidant capacity. Bariatric surgery normalized the composition of lipoproteins and improved the qualitative characteristics of LDL and HDL. In summary, patients with obesity present multiple alterations in the qualitative properties of lipoproteins compared with healthy subjects. Bariatric surgery reverted most of these alterations.

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Effect of Medialization of the Trochlear Groove and Lateralization of the Tibial Tubercle on TT-TG Distance: A Cross-sectional Study of Dysplastic and Nondysplastic Knees

The American Journal of Sports Medicine ◽

10.1177/0363546520987819 ◽

2021 ◽

pp. 036354652098781

Author(s):

Mathias Paiva ◽

Lars Blønd ◽

Per Hölmich ◽

Kristoffer Weisskirchner Barfod

Keyword(s):

Trochlear Dysplasia ◽

Cross Sectional Study ◽

Magnetic Resonance Images ◽

Tibial Tubercle ◽

Control Group ◽

Trochlear Groove ◽

Sectional Study ◽

Cross Sectional ◽

Mean Values ◽

The Mean

Background: Tibial tubercle–trochlear groove (TT-TG) distance is often used as a measure of lateralization of the TT and is important for surgical planning. Purpose: To investigate if increased TT-TG distance measured on axial magnetic resonance images is due to lateralization of the TT or medialization of the TG. Study Design: Cross-sectional study; Level of evidence, 3. Methods: A total of 84 knees (28 normal [NK], 28 with trochlear dysplasia [TD], and 28 with patellar dislocation without TD [PD]) were examined. The medial border of the posterior cruciate ligament (PCL) was chosen as the central anatomic landmark. The distance from the TT to PCL (TT-PCL) was measured to examine the lateralization of the TT. The distance from the TG to the PCL (TG-PCL) was measured to examine the medialization of the TG. Between-group differences were investigated by use of 1-way analysis of variance. Results: The mean values for TT-TG distance were 8.7 ± 3.6 mm for NK, 12.1 ± 6.0 mm for PD, and 16.7 ± 4.3 mm in the TD group ( P < .01). The mean values for TT-PCL distance were 18.5 ± 3.6 mm for NK, 18.5 ± 4.5 mm for PD, and 21.2 ± 4.2 mm in the TD group ( P = .03). The mean values for TG-PCL distance were 9.6 ± 3.0 mm for NK, 7.1 ± 3.4 mm for PD, and 5.1 ± 3.3 mm in the dysplastic group ( P < .01). Conclusion: The present results indicate that increased TT-TG distance is due to medialization of the TG and not lateralization of the TT. Knees with TD had increased TT-TG distance compared with the knees of the control group and the knees with PD. The TT-PCL distance did not differ significantly between groups, whereas the TG-PCL distance declined with increased TT-TG.

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Effect of Microfluidic Sperm Separation vs. Standard Sperm Washing Processes on Laboratory Outcomes and Clinical Pregnancy Rates in an Unselected Patient Population

Reproductive Medicine ◽

10.3390/reprodmed2030013 ◽

2021 ◽

Vol 2 (3) ◽

pp. 125-130

Author(s):

Chelsey A. Leisinger ◽

Glen Adaniya ◽

Melanie R. Freeman ◽

Erica J. Behnke ◽

Martha Aguirre ◽

...

Keyword(s):

Patient Population ◽

Fertilization Rate ◽

Single Embryo Transfer ◽

Clinical Pregnancy ◽

Control Group ◽

Repair Capacity ◽

Separation Device ◽

Unselected Patient ◽

And Control ◽

Sperm Separation

A prospective, multicenter, randomized, sibling oocyte study was conducted with 86 couples to evaluate if a microfluidic sperm separation device improved ICSI sperm selection and subsequent cycle outcomes of fertilization, blastocyst utilization, ploidy, and clinical pregnancy rate when applied to a general patient population. Patients with at least 10 metaphase II oocytes were enrolled in the study and sibling oocyte groups were split in half. One half of the oocytes underwent ICSI with the control processed sperm and the other half were injected with sperm sorted by the ZyMōt microfluidic sperm separation device. Fertilization rate was recorded and resulting blastocysts were biopsied and evaluated for ploidy status with NGS. Euploid, non-mosaic embryos were randomly selected for single embryo transfer. A total of 787 oocytes were evaluated in the ZyMōt group and 777 in the control group. No statistical differences were observed between ZyMōt and control processing methods in any of the study outcomes evaluated. It is possible that the selection of normal, progressive sperm for ICSI, and the repair capacity of oocytes are sufficient to promote normal embryonic development in the general infertility population.

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Association Between Osteoarticular Scores and Acute Phase Reactant Levels in Rheumatoid Arthritis

Journal of Medical Biochemistry ◽

10.2478/v10011-008-0031-2 ◽

2009 ◽

Vol 28 (2) ◽

pp. 116-121 ◽

Cited By ~ 1

Author(s):

Irena Kafeđiska ◽

Dejan Spasovski ◽

Todor Gruev ◽

Mane Grličkov ◽

Kočo Cakalaroski ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Acute Phase ◽

Acute Phase Reactant ◽

Control Group ◽

Time Intervals ◽

Acute Phase Reactants ◽

Mean Values ◽

Cell Counts ◽

Phase Reactant ◽

The Mean

Association Between Osteoarticular Scores and Acute Phase Reactant Levels in Rheumatoid Arthritis The aim of this prospective control study was a quantitative evaluation of the activity of rheumatoid arthritis (RA) in certain time intervals, using articular indexes (set of 28 sensitive and 28 swollen joints), laboratory parameters (Hb, Hct, Er, Le and Plt) and acute phase reactants (ESR, RF, CRP); to determine which of the acute phase reactants is the most useful biochemical marker for the evaluation of disease activity in RA; to quantify the therapeutical and laboratory differences in certain time intervals in the group with and without immunomodulatory therapy with Methotrexate. Sixty patients with RA were included, 27 of who were treated with non-steroid antiinflammatory drugs (NSAIDs) and Methotrexate (MTX). The control group consisted of 33 patients treated only with NSAIDs because of irregular controls. In the first group of patients the disease activity was estimated at four time intervals, and in the control group of patients at three time intervals following the scores of the articular indexes, blood cell counts, ESR and CRP in every patient. In the first group of patients decreased activity of RA was found upon every following control with a consecutive decrease in mean values of the scores of articular indexes with statistically significant differences at the four time intervals. Considering laboratory parameters, there were statistically significant differences in the mean values of Hb, Er, Plt, ESR, (p=0.0462, p=0.0076, p= 0.0058, p= 0.0003). Mean values of CRP did not show statistically significant differences, but the number of patients who were CRP negative increased (there were great standard deviations). In the group of patients treated only with NSAIDs, there were statistically significant differences in the mean values of the scores of articular indexes with an increse at every following control (in favour of progression of the disease). There were no statistically significant differences considering blood cell counts, ESR and CRP (in favour of permanently active disease). In conclusion, CRP is the most useful marker for the prospective follow-up of patients with RA.

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Assessment of Occupational Exposure to BTEX in a Petrochemical Plant via Urinary Biomarkers

Sustainability ◽

10.3390/su13137178 ◽

2021 ◽

Vol 13 (13) ◽

pp. 7178

Author(s):

Višnja Mihajlović ◽

Nenad Grba ◽

Jan Suđi ◽

Diane Eichert ◽

Smilja Krajinović ◽

...

Keyword(s):

Occupational Exposure ◽

Urinary Biomarkers ◽

Working Environment ◽

Passive Samplers ◽

Control Group ◽

Petrochemical Plant ◽

Exposure Limits ◽

Multivariate Statistical ◽

Mean Values ◽

Relative Standard

This work presents the results of the first Serbian monitoring campaign performed to assess the occupational exposure of petrochemical industry workers to benzene (B), toluene (T), ethylbenzene (E), and xylene (X), known collectively as BTEX. The following urinary biomarkers were investigated: phenol, hippuric acid, o-Cresol, p-Cresol, and creatinine. BTEX compounds were collected in 2014 using Casella passive samplers. Multivariate statistical analysis was performed to put in evidence the correlation between the BTEX measured in air and the concentration of urinary biomarkers. While the results indicate an elevated presence of benzene in the air in the working environment studied that surpasses the national and European Occupational Exposure Limits (OEL), the levels of the remaining (TEX) parameters measured were below the OEL. The high relative standard deviations (RSD) for the concentrations of each BTEX compound (68–161 mg m−3) point toward an intensive occupational exposure to BTEX. This was confirmed by relevant urine biomarkers, particularly by the mean values of phenol, which were ten and fourteen times higher than the ones found in the control group (14–12 mg g−1 of creatinine). On average, workers are at a higher risk of developing cancer (6.1 × 10−3), with risk levels exceeding the US EPA limits. Benzene levels should therefore be maintained under tight controls and monitored via proper urinary biomarkers.

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CORRELATION OF SERUM MAGNESIUM LEVELS WITH FASTING PLASMA GLUCOSE IN TYPE 2 DIABETES MELLITUS

10.36106/ijsr/4718559 ◽

2021 ◽

pp. 16-18

Author(s):

Nishanth Kumar ◽

Malathi R D ◽

Ramadevi M

Keyword(s):

Diabetes Mellitus ◽

Plasma Glucose ◽

Fasting Plasma Glucose ◽

Serum Magnesium ◽

Fasting Blood Glucose ◽

Mean Value ◽

Control Group ◽

Mean Values ◽

Fasting Plasma ◽

Increased Risk

Background: Diabetes mellitus is a metabolic disorder of multifactorial origin characterized by hyperglycemia and disturbances of glucose , fat and protein metabolism. Hypomagnesemia is been associated with chronic and uncontrolled diabetes mellitus. Magnesium deciency in diabetes is known to be associated with increased risk of microvascular and macrovascular complications. The aim of this study is to estimate fasting plasma glucose and serum magnesium levels and to assess the correlation of hypomagnesemia with abnormal fasting plasma glucose values. Material and Methods: The study was done at Government Medical College, Nizamabad. 80 subjects were recruited out of whom 40 apparently normal persons were taken as control group and the second group of 40 patients with known history of diabetes. The fasting plasma glucose(FPG) was estimated by GOD-POD method and serum Magnesium(Mg) levels were estimated using the Chemchek Mg kit which is based on Xylidyl Blue with ACTS method. Results :The mean values of fasting plasma glucose was 87.1 mg/dL in non diabetics when compared to 159.4 mg/dL in diabetics while Serum magnesium levels in control subjects had a mean value of 2.19mg/dLand 1.8mg/dLin diabetics. The data was analysed and found to be statistically signicant with a negative correlation between plasma magnesium and fasting blood glucose. Conclusion: There is signicant hypomagnesaemia which correlates increased fasting plasma glucose values in diabetics when compared to non diabetics and therefore assessing the serum magnesium levels may help in reducing risk of complications.

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PHYSIOLOGIC HYPERBILIRUBINEMIA AND HEMOLYSIS OF ERYTHROCYTES BY HYDROGEN PEROXIDE SOLUTION IN THE NEWBORN: INVESTIGATION OF A POSSIBLE RELATIONSHIP

PEDIATRICS ◽

10.1542/peds.20.1.92 ◽

1957 ◽

Vol 20 (1) ◽

pp. 92-97

Author(s):

Jo-Anne E. Richards ◽

Richard B. Goldbloom ◽

Ronald L. Denton

Keyword(s):

Hydrogen Peroxide ◽

Statistical Analysis ◽

Vitamin E ◽

Clinical Significance ◽

Newborn Infant ◽

Newborn Infants ◽

Control Group ◽

Term Infants ◽

Mean Values ◽

Relative Deficiency

Forty-three full-term infants have been studied with respect to hemolysis of erythrocytes in solutions of hydrogen peroxide and concentrations of bilirubin in the serum. Mean values for concentration of bilirubin in the serum and percentage of hemolysis followed similar patterns in the first few days of life. However, statistical analysis of the data in individual cases showed no significant correlation between the degree of hemolysis in solutions of hydrogen peroxide and the concentrations of bilirubin in the serum. Administration of vitamin E prevented an increase in hemolysis of erythrocytes in solutions of hydrogen peroxide but failed to produce any significant change in concentrations of bilirubin as compared with the control group. The evidence suggests that the relative deficiency of vitamin E which exists in most newborn infants does not play a part in the causation or maintenance of physiologic hyperbilirubinemia. The clinical significance of increased hemolysis of the erythrocytes of the newborn infant in solutions of hydrogen peroxide remains a mystery. Possible approaches to the clarification of this problem are suggested.

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Does diffusion-weighted magnetic resonance imaging have a place in the differential diagnosis of brucella sacroiliitis and seronegative spondyloarthropathy?

Acta Radiologica ◽

10.1177/0284185120938370 ◽

2020 ◽

pp. 028418512093837

Author(s):

Sunay Sibel Karayol ◽

Kudret Cem Karayol

Keyword(s):

Magnetic Resonance Imaging ◽

Bone Marrow ◽

Differential Diagnosis ◽

Magnetic Resonance ◽

Control Group ◽

Resonance Imaging ◽

Visual Evaluation ◽

Mean Values ◽

Diffusion Weighted ◽

The Mean

Background The aim of this study is to investigate the role of diffusion-weighted imaging (DWI) in the differential diagnosis of sacroiliitis. Purpose To compare the sacroiliac magnetic resonance imaging (MRI) examinations of patients with suspected active sacroiliitis with patients with acute SpA MR findings and the DWI examinations of patients with acute brucella sacroiliitis, and thereby determine whether DWI can contribute to the differential diagnosis. Material and Methods A total of 84 patients were included in the study and were separated into three groups: group 1 (13 women, 6 men) comprised cases with brucella positive for sacroiliitis; group 2 (17 women, 19 men) comprised cases negative for brucella but with sacroiliitis; and group 3 (16 women, 13 men) comprised cases negative for brucella and sacroiliitis. Results The mean bone marrow apparent diffusion coefficient (ADC) values independently of edema were determined as 0.71 × 10−3 in sacroiliitis and brucella-positive patients, as 0.53 × 10−3 in brucella-negative and sacroiliitis-positive patients, and as 0.43 × 10−3 in the control group of brucella-negative sacroiliitis-negative patients. In the ADC measurements taken from areas of evident edema in patients with sacroiliitis, the mean values were 0.13 × 10−3 in the brucella-positive group and 0.12 × 10−3 in the brucella-negative group. Conclusion By adding DWI, which is a rapid MR sequence, to sacroiliac joint MR examination, normal bone marrow and bone marrow with sacroiliitis can be objectively differentiated with ADC measurements in addition to visual evaluation.

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