scholarly journals GC-MS analysis of cuticular waxes and evaluation of antioxidant and antimicrobial activity of Chaenomeles cathayensis and Ch. × californica fruits

2021 ◽  
Vol 12 (4) ◽  
pp. 718-723
Author(s):  
Y. V. Lykholat ◽  
N. O. Khromykh ◽  
O. O. Didur ◽  
T. V. Sklyar ◽  
T. A. Holubieva ◽  
...  
Keyword(s):  
Octadecenoic Acid ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Cuticular Waxes ◽  
Fruit Peel ◽  
Environmental Threats ◽  
Fruit Extracts ◽  
Antimicrobial Potential ◽  
Antioxidant And Antimicrobial Activity

Fruit extracts of the Chaenomeles species are a rich source of compounds having health-promoting properties, while their distribution between the species and cultivars varies significantly depending on both genotype and environmental threats. This study aimed at discovering antioxidant and antimicrobial potential of the secondary metabolites of fruit and waxes of fruit cuticular of introduced Ch. cathayensis and Ch. × californica plants. The sum of detected polyphenols in the isopropanolic fruit extracts varied slightly between the species, while significant excesses in indices were seen for both species peel extracts as compared to pulp extracts. Antimicrobial assays carried out by disc diffusion method showed notable activity of the fruit peel and pulp extracts of both species against all tested Gram-negative and Gram-positive bacterial strains, and two Candida strains as well. Pseudomonas aeruginosa strain was the most resistant to the action of both fruit extracts, especially peel extracts of Ch. cathayensis fruits. As identified by gas chromatography-mass spectrometry (GC-MS) assays, chloroformic extracts from the fruits of cuticular waxes of Ch. cathayensis and Ch. × californica contained six prevailing fractions: aldehydes, alkanes, alcohols, esters, fatty acids and various terpenoids. The predominant compounds were tetrapentacontane (21.8% of total amount) and heptacosanal (23.1% of total), respectively in the cuticular waxes of Ch. cathayensis and Ch. × californica. Cinnamaldehyde, cis-9-hexadecenal, hexadecanoic acid, oleic acid, olean-12-ene-3,28-diol (3. beta), lupeol, diisooctyl phthalate, 9-octadecenoic acid, 1,2,3-propanetriyl ester, 1,3,12-nonadecatriene-5,14-diol and some other identified compounds are well-known for their bioactivity, indicating the feasibility of studying the antimicrobial potential of plant fruits.

scholarly journals The First Report on Chemical Composition and Antimicrobial Activity of Artemisia scoparia Waldst. et Kit. Extracts

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091503 ◽  
Author(s):  
Gordana S. Stojanović ◽  
Jovana D. Ickovski ◽  
Aleksandra S. Đorđević ◽  
Goran M. Petrović ◽  
Katarina D. Stepić ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Volatile Components ◽  
Bacterial Strains ◽  
First Report ◽  
Main Compound ◽  
Artemisia Scoparia

Volatiles of diethyl ether extract (DE), ethyl acetate extract (EE), and hexane extract (HE) of Artemisia scoparia Waldst. et Kit. were analyzed by gas chromatography with flame ionization detector and gas chromatography-mass spectrometry. In both DE and EE, the main compound was scoparone (24.0% and 86.1%, respectively) while in the HE, alkanes were dominant with nonacosane as the most represented (19.4%). Antimicrobial activity was tested against 4 bacterial strains and 1 fungal strain using disc-diffusion method. Tested samples were inactive against Gram-negative bacteria and they exhibited activity against Gram-positive bacteria and yeast Candida albicans. This is the first report on the chemical composition of volatile components and antimicrobial activity of DE, EE, and HE of A. scoparia Waldst. et Kit.

Chemical composition, antioxidant, and antimicrobial activities of two essential oils from Algerian propolis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Safia Boulechfar ◽  
Amar Zellagui ◽  
Meltem Asan-Ozusaglam ◽  
Chawki Bensouici ◽  
Ramazan Erenler ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Reducing Ability ◽  
Antioxidant And Antimicrobial Activity ◽  
Fungistatic Effect

Abstract This study aims to investigate the chemical composition, antioxidant, and antimicrobial activity of two essential oils (EOs) from Algerian propolis. The volatile constituents were analyzed by gas chromatography-mass spectrometry. Fifty components were identified from the oils. The major components were found to be: cedrol (17.0%), β-eudesmol (7.7%), and α-eudesmol (6.7%) in EO of propolis from Oum El Bouaghi (EOPO) whilst α-pinene (56.1%), cis-verbenol (6.0%), and cyclohexene,3-acetoxy-4-(1-hydroxy-1-methylethyl)-1-methyl (4.4%) in EO of propolis from Batna (EOPB). The antioxidant properties of EOPO and EOPB were determined using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) and cupric reducing antioxidant capacity (CUPRAC assays), respectively. Both EOs had more cupric ion reducing ability than scavenging ABTS•+ radicals. The antimicrobial potential of the two EOs against eight pathogens was assayed by the agar diffusion method and the mode of action was determined by microdilution assay. The results revealed that EOPB was bactericidal for all tested pathogenic bacteria and fungicidal for Candida albicans ATCC 10231, whereas, EOPO showed bacteriostatic effect against Escherichia coli O157:H7 and Pseudomonas aeruginosa ATCC27853 and fungistatic effect against C. albicans ATCC 10231. Thus, the obtained results suggest the important use of propolis EOs as preservative agents.

scholarly journals ANTIBACTERIAL CAPACITY AND IDENTIFICATION OF BIOACTIVE COMPOUNDS BY GCMS OF ALLIUM CEPA

2018 ◽  
Vol 10 (2) ◽  
pp. 116 ◽  
Author(s):  
Dushyant Sharma ◽  
Reena Rani ◽  
Monika Chaturvedi ◽  
J. P. Yadav
Keyword(s):  
Antibacterial Activity ◽  
Allium Cepa ◽  
Shigella Flexneri ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Klebsiella Pneumonia ◽  
Phytochemical Analysis ◽  
Dodecanoic Acid ◽  
Bacterial Strains ◽  
Zone Of Inhibition

Objective: Plants offer a novel source for the isolation of a wide variety of medicinal agents. Allium cepa commonly known as onion is very well known medicinal plants and we investigated the antibacterial activity of different extracts and their phytochemical analysis by gas chromatography mass spectrometry (GCMS).Methods: The extracts of A. cepa prepared in six different solvents was analyzed for antibacterial activity against nine American type cell culture (ATCC) reference bacterial strains i.e. Shigella flexneri, Enterococcus faecalis, Staphylococus aureus, Proteus mirabilis, Salmonella typhi, Serratia marcescens, Klebsiella pneumonia, Escherichia coli, Pseudomonas aeruginosa by using the agar well diffusion method. GCMS analysis also has been carried out for their phytochemical analysis.Results: The results obtained from agar well diffusion assay showed the zone of inhibition range from 10±0.76 to 26±0.76 mm for different extracts. The methanol extract was found most potent against K. pneumonia and S. marcescens with the zone of inhibition of 26±0.76 mm for both strains. Minimum inhibitory concentration (MIC) values were in the range of 1.87 to 7.5 mg/ml and the MIC values for K. pneumonia and S. marcescens was 1.87 mg/ml. A total of 43 compounds were identified by GCMS analysis. Out of them dodecanoic acid was found common in all extracts.Conclusion: It is concluded that Allium cepa have good antibacterial activity so it can be used for the treatment of various infectious diseases.

scholarly journals Evaluation of Antibacterial Activity and GCMS Analysis of Zanthoxylum Ovalifolium Fruit Extracts

2021 ◽  
pp. 7-17
Author(s):  
P. Pavani ◽  
Raja Naika
Keyword(s):  
Bioactive Compounds ◽  
Octadecenoic Acid ◽  
Ethyl Oleate ◽  
Herbal Medicines ◽  
Vital Role ◽  
Diffusion Method ◽  
Molecular Formula ◽  
Fruit Extract ◽  
E Coli ◽  
Fruit Extracts

Herbal medicines have played a vital role in the evolution of humanity and civilization. The fruits of the plant Zanthoxylum were collected from the study field, washed, dried in the shade and powdered. Soxhlation method was used to make different solvent extract of fruit. The different fruit extracts were examined against P. aeruginosa, P. syringe, S. aureus, S. typhi, K. pneumonia and E. coli by agar well diffusion method and for the detection of biochemical components present in the Zanthoxylum fruit, methanolic fruit extract was analyzed using GC-MS. Results showed that, the methanolic extract of fruit is found to be exhibit activity against P. aeruginosa, P. syringe, S. aureus, S. typhi, K. pneumonia and E. coli when compared to the hexane and ethyl acetate. The discovery of 46 bioactive compounds in methanolic fruit extract was verified by their molecular formula, molecular weight, and area peak percentage. In which 6 bioactive compounds such as 6-octadecanoic acid, Pentadecanoic acid, Ethyl oleate, 9-octadecenoic acid (Z)-, methyl ester, Glycerin and 1, 2-dimethoxy-4-(2-methoxyethenyl) benzene compounds showed highest peak area percentage of 26.14, 13.61, 8.56, 6.12 and 6.08% respectively. The compounds found in this research may be responsible for some of the medicinal action of the Z. ovalifolium fruit and may be useful in the development of new medicines.

scholarly journals ANTIMICROBIAL POTENTIAL OF HERBAL AND CHEMICAL NEONATAL EYE DROPS

2018 ◽  
Vol 11 (11) ◽  
pp. 319
Author(s):  
Nitika Thakur ◽  
Jyoti Kumari ◽  
Monu Sharma
Keyword(s):  
Bacterial Infections ◽  
Fungal Infections ◽  
Diffusion Method ◽  
Chemical Components ◽  
Dilution Method ◽  
Eye Drops ◽  
Bacterial Strains ◽  
Antimicrobial Potential ◽  
Good Antibacterial Activity ◽  
Broth Dilution

Objective: The present study focuses on exploring the antimicrobial potential of chemical (Tobastar and natamycin) and herbal eye drops (Itone) which are specifically preferred against neonatal eye infections.Methods: Well diffusion method (for testing the antimicrobial potential of selected eye drops against different bacterial and fungal strains), minimum inhibitory concentration (MIC) (broth dilution method) for detecting the MIC effective against various strains taken into consideration.Results: The results indicated that Itone and Tobastar are effective against bacterial strains, thus highlighting the importance of these two eye drops against bacterial infections, whereas natamycin is effective against fungal infection.Conclusion: It may be concluded that the herbal eye drop (Itone) and the chemical eye drop (Tobastar) are effective against bacterial infections. The components such as neem, tulsi, kapur, and honey in herbal composition possess good antibacterial activity confirming the great potential of bioactive compounds and are useful for the growth inhibition of the carcinogenic bacterium. The alkaloids, flavonoids and saponins are based on antibiotic principles and are actually the defensive mechanisms of the plant against pathogens and chemical eye drop, that is, natamycin is effective against fungal infections, which may be due to the presence of polyene antifungal agent which works against fungal infections. There is a need to optimize the blending of the different herbal and chemical components to form a single effective antimicrobial formulation (more of herbal component and less chemical components) against neonatal infections.

scholarly journals Antimicrobial Potential and Chemical Characterization of Serbian Liverwort (Porella arboris-vitae): SEM and TEM Observations

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Amit Kumar Tyagi ◽  
Danka Bukvicki ◽  
Davide Gottardi ◽  
Milan Veljic ◽  
Maria Elisabetta Guerzoni ◽  
...  
Keyword(s):  
Electron Microscope ◽  
Antimicrobial Agents ◽  
Solid Phase ◽  
Chemical Characterization ◽  
Ethanol Extract ◽  
Food Preservation ◽  
Gas Chromatography Mass Spectrometry ◽  
Dilution Method ◽  
Bacterial Strains ◽  
Antimicrobial Potential

The chemical composition ofPorella arboris-vitaeextracts was determined by solid phase microextraction, gas chromatography-mass spectrometry (SPME GC-MS), and 66 constituents were identified. The dominant compounds in methanol extract ofP.arboris-vitaewereβ-caryophyllene (14.7%),α-gurjunene (10.9%),α-selinene (10.8%),β-elemene (5.6%),γ-muurolene (4.6%), and allo-aromadendrene (4.3%) and in ethanol extract,β-caryophyllene (11.8%),α-selinene (9.6%),α-gurjunene (9.4%), isopentyl alcohol (8.8%), 2-hexanol (3.7%),β-elemene (3.7%), allo-aromadendrene (3.7%), andγ-muurolene (3.3%) were the major components. In ethyl acetate extract ofP.arboris-vitae, undecane (11.3%),β-caryophyllene (8.4%), dodecane (6.4%),α-gurjunene (6%), 2-methyldecane (5.1%), hemimellitene (4.9%), and D-limonene (3.9%) were major components. The antimicrobial activity of differentP.arboris-vitaeextracts was evaluated against selected food spoilage microorganisms using microbroth dilution method. The Minimal Inhibitory Concentration (MIC) varied from 0.5 to 1.5 mg/mL and 1.25 to 2 mg/mL for yeast and bacterial strains, respectively. Significant morphological and ultrastructural alterations due to the effect of methanolic and ethanolicP.arboris-vitaeextracts onS. Enteritidis have also been observed by scanning electron microscope and transmission electron microscope, respectively. The results provide the evidence of antimicrobial potential ofP.arboris-vitaeextracts and suggest its potential as natural antimicrobial agents for food preservation.

scholarly journals Soluble cuticular wax composition and antimicrobial activity of the fruits of Chaenomeles species and an interspecific hybrid

2021 ◽  
Vol 29 (4) ◽  
pp. 334-339
Author(s):  
Y. V. Lykholat ◽  
N. O. Khromykh ◽  
O. O. Didur ◽  
S. I. Okovytyy ◽  
T. V. Sklyar ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bioactive Compounds ◽  
Interspecific Hybrid ◽  
Steppe Zone ◽  
Biologically Active ◽  
Gas Chromatography Mass Spectrometry ◽  
Cuticular Waxes ◽  
Fruit Extracts ◽  
Gram Positive Bacteria ◽  
Health Promoting

Plants of the genus Chaenomeles Lindl. (Rosaceae) naturally grow in Southeast Asia and represent the richest resource of biologically active compounds with beneficial properties for humans. Plants of C. japonica (Thunb.) Lindl. and C. speciosa (Sweet) Nakai species, and interspecific hybrid C. × superba (Frahm) Rehder (C. japonica × C. speciosa, Superba group) have been successfully introduced in the steppe zone of Ukraine and bear fruits. In this study, we evaluated chemical composition of fruit cuticular waxes and antimicrobial activity of fruit extracts. The soluble waxes were characterized using gas chromatography-mass spectrometry (GC-MS), and 26–36 compounds, representing 91.7–96.6% of the total soluble cuticular waxes, were identified. Waxes of Chaenomeles fruits belonged to six classes, namely fatty acids, alcohols, aldehydes, esters, ethers and alkanes. Aldehydes 7-hexadecenal and heptacosanal, and alkanes hexatriacontane and tetrapentacontane were the main constituents in the soluble cuticular waxes of C. speciosa and C. × superba fruits, accounting for more than half of the total contents. However, alkane tetrapentacontane, alcohol 8,10-hexadecadien-1-ol and heptacosanal prevailed in C. japonica fruit waxes. Isopropanolic fruit extracts exhibited dose-dependent antimicrobial activity against four Gram-negative bacteria, five Gram-positive bacteria and one fungal strain in the disc diffusion assay. In general, extracts from the Chaenomeles fruits demonstrated higher activity against Gram+ bacteria than Gram- strains. The strongest inhibiting activity was shown against Staphylococcus epidermidis (by the fruit extracts of C. × superba and C. speciosa), Micrococcus lysodeikticus and Candida albicans (both by C. × superba fruit extract). Results of the study confirmed accumulation of the bioactive compounds in the fruit waxes of different Chaenomeles species and antimicrobial ability of Chaenomeles fruits as well. These findings revealed the bioactive compounds in fruit cuticular waxes and suggested health-promoting properties of introduced Chaenomeles species.

Chemical composition and anti-microbial analysis of Mentha arvensis L. and Thymus linearis Benth. essential oils of leaves

2021 ◽  
Vol 26 (5) ◽  
pp. 2893-2900
Author(s):  
SAJIDA BIBI ◽  
◽  
SHAHAB ALI ◽  
SHAHI DIN ◽  
IKRAM ULLAH ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Compositional Data ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Mentha Arvensis ◽  
Microbial Analysis ◽  
First Time

This study was performed to evaluate the chemical composition of the essential oils of Mentha arvensis and Thymus linearis and their antimicrobial activities. The complexity of the essential oils is a basic challenge for determining their reliable and accurate compositional data. Rapid advances in spectroscopic and chromatographic techniques have resolved this challenge to a large extent by examining essential oils. Essential oils were analyzed through Gas Chromatography-Mass Spectrometry (GC-MS) following extraction through steam distillation from their leaves for the first time in Miandam, District Swat, KP, Pakistan. The GC-MS analysis revealed 26 and 25 components in the essential oil of Mentha arvensis and Thymus linearis respectively. The major components were carvone (23.53%), P-Cymen-2-ol (20.35%), and caryophyllene oxide (18.81%) in Mentha arvensis but Thymus linearis has Thymol (2-isopropyl-5-methyl phenol) (40%), O-Cymene (2-Isopropyltoluene) (14.95%) and beta-bisbolene (12.54%). The essential oils of both plants showed bactericidal activities against five different bacterial strains (i.e. Escherichia coli, Staphylococcus aureus, Klesiella pneumonia, Bacillus subtilis, and Pseudomonas aeruginosa) during disc diffusion method and therefore it is suggested that they may be used as a natural antiseptic and could play important roles in food and pharmaceutical industry.

scholarly journals Antibacterial Efficiency of Finnish Spice Essential Oils against Pathogenic and Spoilage Bacteria

2004 ◽  
Vol 67 (1) ◽  
pp. 199-202 ◽  
Author(s):  
MARI NEVAS ◽  
ANNA-RIITTA KORHONEN ◽  
MIIA LINDSTRÖM ◽  
PEKKA TURKKI ◽  
HANNU KORKEALA
Keyword(s):  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antibacterial Properties ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Spectrometry Analysis ◽  
Spoilage Bacteria ◽  
Gram Negative Organisms

The antibacterial properties of 13 essential oils, derived from spices grown in Finland, were examined with an agar diffusion method against 12 bacterial strains. The organisms tested included both spoilage and pathogenic bacteria. The gram-positive bacteria appeared to be more sensitive than the gram-negative organisms, Clostridium botulinum and Clostridium perfringens being the most sensitive. Oregano, savory, and thyme showed the broadest antibacterial activity by distinctly inhibiting the growth of all the organisms tested. By gas chromatography–mass spectrometry analysis, differences were noted in the composition of oregano and thyme oils in comparison to previous reports.

scholarly journals The Synergistic Effect of Three Essential Oils against Bacteria Responsible for the Development of Lithiasis Infection: An Optimization by the Mixture Design

2021 ◽  
Vol 2021 ◽  
pp. 1-17
Author(s):  
Rabie Kachkoul ◽  
Ghita Benjelloun Touimi ◽  
Bahia Bennani ◽  
Radouane El Habbani ◽  
Ghita El Mouhri ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Essential Oils ◽  
Negative Impact ◽  
Mixture Design ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Mentha Pulegium ◽  
Minimal Inhibitory Concentrations ◽  
Tract Infections

The present study aimed to determine the chemical composition and the synergistic effect of three plants’ essential oils (EOs), Eucalyptus camaldulensis (ECEO), Mentha pulegium (MPEO), and Rosmarinus officinalis (ROEO), against three bacterial strains, Proteus mirabilis, Klebsiella pneumoniae, and Staphylococcus aureus, in order to increase the antimicrobial effectiveness by the use of a low dose of essential oils, consequently decreasing the toxicity and negative impact. For this reason, an augmented simplex-centroid mixture design was used to build polynomial models in order to highlight the synergy between the essential oils against bacterial strains. Antimicrobial effect screening was performed by the disc diffusion method and the minimal inhibitory concentrations (MIC) were also studied. The gas chromatography/mass spectrometry (GC-MS) results show the richness of these essential oils by terpenic compounds, especially 1,8-Cineole and P-Cymene for ECEO, Pulegone for MPEO, and α -Pinene and Camphene for ROEO. Moreover, a significant antibacterial effect has been demonstrated and the best values were revealed by MPEO and ECEO against P. mirabilis and K. pneumoniae, with inhibition zones (IZ) of 25 and 20 mm, respectively, and an MIC of 0.0391% (v:v) against K. pneumoniae. The optimal mixtures showed a synergistic effect of essential oils, and the lowest minimal inhibitory concentrations of the mixtures (MICm) were in the order of 29.38% of MPEO, 45.37% of ECEO, and 25.25% of ROEO against P. mirabilis and in the order of 60.61% of MPEO and 39.39% of ROEO against K. pneumoniae. These results indicate the antibacterial efficacy of the three essential oils combined and suggest their importance in the treatment of urinary tract infections caused by resistant bacterial strains.

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