scholarly journals Evaluation of the antimicrobial and anti-Varroa destructor L. activity of the essential oil of clove (Syzygium aromaticum L. Myrtaceae)

2021 ◽  
Vol 13 (4) ◽  
pp. 404-408
Author(s):  
K. Mekademi ◽  
D. Kebour ◽  
N.A. Ouchene-Khelifi ◽  
N. Ouchene
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Essential Oil ◽  
Inhibitory Activity ◽  
Varroa Destructor ◽  
Inhibition Zone ◽  
Syzygium Aromaticum ◽  
Penicillium Sp ◽  
Natural Treatment

Abstract. Antimicrobial and anti-Varroa destructor L. activity of the essential oil (EO) of clove (Syzygium aromaticum L. Myrtaceae) was evaluated in this study. Antimicrobial activity concerned 9 bacterial strains (Bacillus cereus, Escherichia coli ATCC 25911, Staphylococcus aureus 29213 ATCC, Proteus mirabilis, Serratia marcescens, Enterobacter sakazakii, Pseudomonas aeruginosa ATCC, Escherichia coli and Acinetobacter sp.), a fungal strain (Penicillium sp.) and a yeast species (Candida albicans). The extraction of the EO was carried out by the method of hydrodistillation. Results showed that EO has no toxicity on bees Apis mellifera. EO demonstrated effective and stable anti-V. destructor activity, indicating the absence of possible resistance, in contrast to what was observed for Amitraz. The inhibitory activity of EO revealed an inhibition zone of diameter varying between 20 mm and 42 mm for bacteria. However, no inhibition zones were observed for Pseudomonas aeruginosa ATCC. The inhibitory activity of EO on Penicillium sp and Candida albicans revealed an inhibition zone of 39 mm in diameter. This study shows that the EO of cloves constitutes a simple and natural treatment, without inconveniences, with a high activity antimicrobial and anti-Varroa destructor which merits it to be proposed as a means to fight against varroosis and the tested pathogens.

scholarly journals Antimicrobial effect of raw garlic (allium sativum L.) extracts, garlic powder and oil and commercial antibiotics on pathogen microorganisms

2004 ◽  
Vol 58 (3) ◽  
pp. 109-113 ◽  
Author(s):  
Vesna Nikolic ◽  
Mihajlo Stankovic ◽  
Ljubisa Nikolic ◽  
Dragan Cvetkovic ◽  
Dejan Skala
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Essential Oil ◽  
Antimicrobial Effect ◽  
Aqueous Extracts ◽  
Commercial Preparation ◽  
Garlic Powder

Garlic powder, essential oil and aqueous extracts from raw garlic and garlic powder prepared in accordance with the corresponding procedures were tested for antimicrobial activity on Staphylococcus aureus ATCC 6538, Escherichia coli ATCC 25922, Bacillus subtilis 6633, Sarcina lutea ATCC 4391, Pseudomonas aeruginosa ATCC 9027, Salmonella enteritidis, Klebsiel la pneumoniae, Proteus vulgaris, Candida albicans ATCC 10231 and Aspergillus niger ATCC 16404; and, three pathogen microorganisms isolated from human material were also used: Staphylococcus aureus, Escherichia coli and Candida albicans. The antimicrobial activities of the preparations were compared with that of commercial antibiotics and four commercial preparation based on garlic powder and essential oil. Aqueous extracts from raw garlic, from freshly prepared powder and powder stored for 18 months at +4?had the greatest antibacterial and antimycotic activities on all the microorganisms tested. The essential oil obtained by hydro distillation and commercial preparations based on garlic essential oil showed no antimicrobial activity on the microorganisms tested. Thermal oils showed a bacteriostatic or very weak bacteriostatic effect on all the microorganisms except Pseudomonas aeruginosa. The garlic powder and commercial preparations based on the powder have a strong antimicrobial effect on all the microbes except on Pseudomonas aeruginosa.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Antimicrobial activity of Syzygium aromaticum L. essential oil on extended-spectrum beta-lactamases-producing Escherichia coli

2020 ◽  
Vol 44 (1) ◽  
Author(s):  
E. L. Mejía-Argueta ◽  
J. G. Santillán-Benítez ◽  
M. M. Canales-Martinez ◽  
A. Mendoza-Medellín
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Genetic Material ◽  
Gastrointestinal Diseases ◽  
Syzygium Aromaticum ◽  
Antibiotic Resistant ◽  
Plant Essential Oils ◽  
E Coli ◽  
Extended Spectrum ◽  
Tract Infections

Abstract Background To test the antimicrobial potential of clove essential oil that has been less investigated on antimicrobial-resistant organisms (extended-spectrum β-lactamase-ESBL-producing Escherichia coli), we collected 135 ESBL-producing Escherichia coli strains given that E. coli is the major organism increasingly isolated as a cause of complicated urinary and gastrointestinal tract infections, which remains an important cause of therapy failure with antibiotics for the medical sector. Then, in this study, we evaluated the relationship between the antibacterial potential activity of Syzygium aromaticum essential oil (EOSA) and the expression of antibiotic-resistant genes (SHV-2, TEM-20) in plasmidic DNA on ESBL-producing E. coli using RT-PCR technique. Results EOSA was obtained by hydrodistillation. Using Kirby-Baüer method, we found that EOSA presented a smaller media (mean = 15.59 mm) in comparison with chloramphenicol (mean = 17.73 mm). Thus, there were significant differences (p < 0.0001). Furthermore, EOSA had an antibacterial activity, particularly on ECB132 (MIC: 10.0 mg/mL and MBC: 80.0 mg/mL), and a bacteriostatic effect by bactericidal kinetic. We found that the expression of antibiotic-resistant gene blaTEM-20 was 23.52% (4/17 strains) and no expression of blaSHV-2. EOSA presented such as majority compounds (eugenol, caryophyllene) using the GC–MS technique. Conclusions Plant essential oils and their active ingredients have potentially high bioactivity against a different target (membranes, cytoplasm, genetic material). In this research, EOSA might become an important adjuvant against urinary and gastrointestinal diseases caused by ESBL-producing E. coli.

scholarly journals СИНТЕЗ ТА ДОСЛІДЖЕННЯ АНТИМІКРОБНОЇ АКТИВНОСТІ ДЕЯКИХ ПІРАЗОЛЗАМІЩЕНИХ 7H-[1,2,4]ТРИАЗОЛО[3,4-B][1,3,4]ТІАДІАЗИНІВ

2021 ◽  
pp. 5-13
Author(s):  
I. I. Myrko ◽  
T. I. Chaban ◽  
V. V. Ogurtsov ◽  
V. S. Matiychuk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Drug Discovery ◽  
Klebsiella Pneumoniae ◽  
Acinetobacter Baumannii ◽  
Cryptococcus Neoformans ◽  
Antimicrobial Drug

Мета роботи. Здійснити синтез деяких нових піразолзаміщених 7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазинів та провести дослідження антимікробних властивостей синтезованих сполук. Матеріали і методи. Органічний синтез, ЯМР-спектроскопія, елементний аналіз, фармакологічний скринінг. Результати й обговорення. У результаті взаємодії eтил (2Z)-хлоро(фенілгідразоно)ацетатів з ацетилацетоном було отримано етил 4-ацетил-5-метил-1-феніл-1H-піразол-3-карбоксилати. Зазначені сполуки піддали бромуванню, що дозволило одержати цільові бромкетони. Синтезовані на даній стадії етил 1-арил-4-(бромацетил)-5-метил-1Н-піразол-3-карбоксилати було введено у взаємодію з 4-аміно-5-арил(гетарил)-2,4-дигідро-3Н-1,2,4-триазол-3-тіонами з подальшим формуванням 1,3,4-тіадіазольного циклу та отриманням відповідних етил 1-арил-4-{3-арил(гетарил)-7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазин-6-іл)}-5-метил-1H-піразол-3-карбоксилатів. Структура синтезованих сполук підтверджена даними елементного аналізу та ЯМР спектроскопією. В рамках міжнародного проекту "The Community for Antimicrobial Drug Discovery" (CO-ADD) за підтримки Wellcome Trust (Великобританія) і університету Квінсленда (Австралія) для синтезованих сполук здійснено скринінг антимікробної активності. Як тестові мікроорганізми використовували п'ять штамів бактерій: Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 700603, Acinetobacter baumannii ATCC 19606, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 43300 та двох штамів грибків: Candida albicans ATCC 90028 і Cryptococcus neoformans ATCC 208821. Встановлено, що досліджувані сполуки виявляють різноманітну дію, від практично повної її відсутності до виразного антимікробного ефекту. Висновки. Здійснено синтез 12 нових етил 1-арил-4-{3-арил(гетарил)-7H-[1,2,4]триазоло[3,4-b][1,3,4]тіадіазин-6-іл)}-5-метил-1H-піразол-3-карбоксилатів. Зазначені речовини отримані шляхом взаємодії відповідних етил 1-арил-4-(бромацетил)-5-метил-1Н-піразол-3-карбоксилатів з 4-аміно-5-арил(гетарил)-2,4-дигідро-3Н-1,2,4-триазол-3-тіонами. Дослідження антимікробної активності синтезованих сполук демонструють потенціал пошуку антимікробних агентів серед зазначеного класу сполук.

scholarly journals UTILIZAÇÃO DE GÁS OZÔNIO NA DESINFECÇÃO DE RESÍDUOS DE SERVIÇOS DE SAÚDE

2018 ◽  
Vol 7 (2) ◽  
pp. 125-139
Author(s):  
Thais Nogueira Gonzaga ◽  
Dora Inés Kozusny-Andreani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Health Care Services ◽  
Pathogenic Bacteria ◽  
Ozone Treatment ◽  
Microbiological Analysis ◽  
Before And After ◽  
Staphylococcus Spp

Nesta pesquisa objetivou-se avaliar a viabilidade técnica da aplicação de ozônio como bactericida e fungicida em amostras de resíduos de serviços de saúde potencialmente infectantes. Foram determinados os     micro-organismos presentes nos resíduos gerados em um hospital particular. Para realização das análises microbiológicas e o tratamento com ozônio o material foi particulado e homogeneizado. As análises microbiológicas foram realizadas antes e após a ozonização.Para os testes de desinfecção foram retirados 10,0g de amostra que foi submetida à ozonização por 5, 10, 15, 20 e 25 minutos com doses de 140,0; 280,0; 420,0; 560,0 e 700,0mg L-1 de ozônio, respectivamente. Verificou-se presença de mesófilos totais, coliformes totais e termotolerantes, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans e Rhizopus spp. O ozônio foi eficiente para eliminação de todos os micro-organismos em 20 minutos; nos primeiros cinco minutos de exposição ao gás verificou-se redução superior a 98%.Palavras-chave: Bactérias patogênicas. Fungos. Ozonização. USING OZONE GAS FOR DISINFECTION OF SOLID WASTE FROM HEALTH CARE SERVICES ABSTRACT: The aim of this research was to evaluate the technical viability of the application of ozone as bactericide and fungicide in samples of potentially infectious health services residues. The microorganisms present in the waste generated in a private hospital were determined. The material was particulated and homogenized to perform the microbiological analysis and to undergo ozone treatment. Microbiological analysis was performed before and after ozonization. For the disinfection tests, 10.0g of sample were removed and submitted to ozonization for 5, 10, 15, 20 and 25 minutes with 140,0; 280,0; 420,0; 560,0 and 700,0mg doses of L-1 of ozone, respectively. It was verified the presence of total mesophiles, total and thermotolerant coliforms, Escherichia coli, Pseudomonas aeruginosa, Proteus spp., Staphylococcus aureus, Staphylococcus spp, Candida albicans and Rhizopus spp. Ozone was efficient while eliminating all microorganisms in 20 minutes; in the first five minutes of gas exposure, the reduction was greater than 98%.Keywords: Pathogenic bacteria. Fungi. Ozonization.

scholarly journals Determinação da microbiota presente na cloaca e orofaringe de avestruzes (Struthio camelus) clinicamente sadios

2004 ◽  
Vol 34 (6) ◽  
pp. 1871-1876 ◽  
Author(s):  
Priscilla Anne Melville ◽  
Bruno Cogliati ◽  
Maria Bárbara Baptista Cepellos Daruiz Mangiaterra ◽  
Monica Ruz Peres ◽  
Sílvio Carlos Alves Moura ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Struthio Camelus ◽  
E Coli ◽  
Bacillus Spp

O conhecimento da microbiota que compõe as diferentes áreas do organismo tem importância reconhecida para a compreensão de doenças infecciosas que podem acometer os avestruzes, embora se disponha de dados limitados acerca deste assunto na literatura. Foi objetivo deste estudo determinar as espécies de microrganismos (bactérias aeróbias e fungos) que compõem a microbiota normal de avestruzes. Para tanto, foram coletadas amostras de cloaca (N=50) e orofaringe (N=50) de avestruzes hígidos de um criadouro. Das amostras de cloaca, foram isolados Escherichia coli (76% das amostras positivas), Bacillus spp. (60%), Streptococcus spp. (18%), Staphylococcus coagulase-negativo (16%), Pseudomonas aeruginosa (8%), Rhodotorula spp. (8%), dentre outros microrganismos isolados em cultura pura ou em associação com outras bactérias e/ou fungos. Das amostras de orofaringe, foram isolados E. coli (74% das amostras positivas), Candida albicans (44%), Bacillus spp. (38%), Staphylococcus coagulase-negativo (32%), Klebsiella pneumoniae (32%), Rhodotorula spp. (8%), Criptococcus spp. (4%), dentre outros microrganismos isolados em cultura pura ou em associação com outras bactérias e/ou fungos. Verificou-se predominância de bactérias Gram negativas em relação às Gram positivas, nas microbiotas da cloaca e orofaringe. Verificou-se freqüência de ocorrência semelhante entre bactérias Gram negativas nas microbiotas da cloaca e orofaringe, bem como de bactérias Gram positivas nestes mesmos sítios. Observou-se maior ocorrência de leveduras em amostras de orofaringe quando comparadas com as de cloaca.

scholarly journals Antibacterial Activity and Mechanism of Ginger Essential Oil against Escherichia coli and Staphylococcus aureus

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3955 ◽  
Author(s):  
Xin Wang ◽  
Yi Shen ◽  
Kiran Thakur ◽  
Jinzhi Han ◽  
Jian-Guo Zhang ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Cell Membrane ◽  
Bacterial Cell ◽  
Tricarboxylic Acid Cycle ◽  
Inhibition Zone ◽  
Underlying Mechanism ◽  
Bacterial Suspension

Though essential oils exhibit antibacterial activity against food pathogens, their underlying mechanism is understudied. We extracted ginger essential oil (GEO) using supercritical CO2 and steam distillation. A chemical composition comparison by GC-MS showed that the main components of the extracted GEOs were zingiberene and α-curcumene. Their antibacterial activity and associated mechanism against Staphylococcus aureus and Escherichia coli were investigated. The diameter of inhibition zone (DIZ) of GEO against S. aureus was 17.1 mm, with a minimum inhibition concentration (MIC) of 1.0 mg/mL, and minimum bactericide concentration (MBC) of 2.0 mg/mL. For E. coli, the DIZ was 12.3 mm with MIC and MBC values of 2.0 mg/mL and 4.0 mg/mL, respectively. The SDS-PAGE analysis revealed that some of the electrophoretic bacterial cell proteins bands disappeared with the increase in GEO concentration. Consequently, the nucleic acids content of bacterial suspension was raised significantly and the metabolic activity of bacteria was markedly decreased. GEO could thus inhibit the expression of some genes linked to bacterial energy metabolism, tricarboxylic acid cycle, cell membrane-related proteins, and DNA metabolism. Our findings speculate the bactericidal effects of GEO primarily through disruption of the bacterial cell membrane indicating its suitability in food perseveration.

scholarly journals ANÁLISE DA COR E DA ATIVIDADE ANTIMICROBIANA DE MICROCÁPSULAS CONTENDO LACTOFERRINA BOVINA

2014 ◽  
Vol 32 (2) ◽  
Author(s):  
LUCIANA HELENA MAIA PORTE ◽  
MARIA HELENA MIGUEZ ROCHA LEÃO ◽  
ALEXANDRE PORTE
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Spray Drying ◽  
Enterococcus Faecium ◽  
Micrococcus Luteus ◽  
Rhodococcus Equi ◽  
Streptococcus Faecium

Lactoferrina bovina (bLF), proveniente do soro de leite, foimicroencapsulada pela técnica de spray drying. Microcápsulascontendo 20 % de bLF foram produzidas, utilizando-se comomaterial de parede dextrina: amido octenilsuccinato (OSA) emdiferentes proporções: 100:00, 75:25, 50:50, 25:75 e 0:100 %.Foram avaliadas a cor e a estabilidade de cor das microcápsulassob armazenamento em ambientes com diferentes umidadesrelativas e a atividade antimicrobiana da lactoferrina liberadadas microcápsulas. As microcápsulas apresentaram cor clara etenderam a escurecer sob armazenamento em ambiente com altaumidade relativa. Verifi cou-se atividade inibitória das microcápsulasde bLF produzidas para diferentes bactérias Gram positivas(Bacillus subtilis CCT 2576, Staphylococcus aureus CCT 2740,Micrococcus luteus CCT 2692, Enterococcus faecium CCT 5079,Streptococcus faecium ATCC 10541, Rhodococcus equi CCT0541), Gram negativas (Pseudomonas aeruginosa ATCC 13388,Salmonella choleraesius CCT 4296, Escherichia coli CCT 0547) elevedura (Candida albicans ATCC 10231). A concentração inibitóriamínima (MIC) das microcápsulas variou de acordo com o microorganismotestado (MIC entre 2,5-100 mg.mL-1). Com exceção deB. subtilis (MIC entre 50-100 mg.mL-1 para as microcápsulas), aconcentração de bLF contida nas microcápsulas necessária parainibir o crescimento dos micro-organismos foi menor do que a bLFnativa. Esses resultados sugerem efeito de potencialização daatividade antimicrobiana da bLF após o processamento por spraydrying.

Biogenic Synthesis of AgNPs with Saussurea lappa C.B. Clarke and Studies on Their Biochemical Properties

2018 ◽  
Vol 18 (12) ◽  
pp. 8392-8398 ◽  
Author(s):  
Muhammad Riaz ◽  
Muhammad Altaf ◽  
Amir Faisal ◽  
Muhammad Azhar Shekheli ◽  
Ghulam Abbass Miana ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Inhibition Zone ◽  
Xrd Analysis ◽  
Biochemical Properties ◽  
Root Extract ◽  
Biogenic Synthesis ◽  
Synthesis Of Nanoparticles ◽  
Saussurea Lappa ◽  
Drug Candidates

Nanotechnology has become an irreplaceable need and green synthesis of nanoparticles offers several advantages over physical and chemical methods. Medicinal plants are the main reservoirs of drugs and drug candidates. We report the biogenic synthesis of silver nanoparticles (AgNPs) using aqueous root extract of Saussurea lappa. Verification and characterization of these nanoparticles were done by UV-visible spectroscopy, XRD-analysis and Scanning Electron Microscopy and FT-IR. Extract-loaded-AgNPs showed the highest inhibition zone against Escherichia coli (11.0 mm) and intermediate against Pseudomonas aeruginosa (9.0 mm). The methanolic root extract of S. lappa alone, also moderately inhibited Pseudomonas aeruginosa (9.0 mm) and showed lower activity (6.0 mm) against Escherichia coli. Its aqueous roots extract inhibited (6.0 mm) the growth of tested organisms. Methanolic extract showed antioxidant potency (IC50 = 0.814 μg/mL). Experiments revealed the presence of phenols and flavonoids in the roots of Saussurea lappa. These findings provide promising interest to exploit Saussurea lappa for the biogenic synthesis of AgNPs and their biological applications.

scholarly journals Componentes químicos y actividad antimicrobiana del aceite esencial de Lasiocephalus ovatus (Asteraceae) que crece en Ecuador

2020 ◽  
Vol 25 (1) ◽  
pp. 22-28
Author(s):  
Liliana Margarita Araujo Baptista ◽  
Katerine Vimos-Sisa ◽  
Rosa Cruz-Tenempaguay ◽  
Félix Falconí-Ontaneda ◽  
Luis Rojas-Fermín ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis

Ecuador es uno de los países más ricos en biodiversidad y endemismo del mundo, y cerca de 3200 especies de plantas tienen usos medicinales. El objetivo de esta investigación fue evaluar la composición química y el efecto antimicrobiano del aceite esencial de Lasiocephalus ovatus Schltdl. (Asteraceae) colectada en la provincia de Chimborazo, Ecuador. Las partes aéreas de L. ovatus fueron sometidas a hidrodestilación para obtener el aceite esencial, el cual fue analizado mediante cromatografía de gases acoplada a espectrometría de masas. La actividad fue evaluada frente a cinco bacterias y una levadura usando la técnica de microdilución en caldo en microplacas de 96 pozos. El rendimiento del aceite fue de 0,05 % y 27 compuestos fueron identificados, representando 95,45 % de la composición total con un elevado contenido de monoterpenos oxigenados (52,17 %). Los compuestos mayoritarios fueron alcanfor (40,48 %) y 1,2,5,5-tetrametil-1,3-ciclopentadieno (11,90 %), seguido por p-menta-1,5-dien-8-ol (5,23 %) y 1,6-dimetilhepta-1,3,5-trieno (4,69 %). Las bacterias más sensibles a la acción del aceite fueron Staphylococcus aureus ATCC 25923 y Escherichia coli ATCC 25922 con concentraciones mínimas inhibitorias de 200-400 μg/mL y bactericidas de 800 μg/mL. La inhibición antimicrobiana frente a las bacterias Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 700603 y la levadura Candida albicans ATCC 10231 fue baja, con un rango de concentración mínima inhibitoria de 800 a 6400 μg/mL. Este reporte representa un primer análisis de la actividad antimicrobiana del aceite de L. ovatus, por lo tanto, una contribución importante al estudio del género Lasiocephalus.

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