Microbiology of plant foods and related aspects

2009 ◽  
Vol 38 (Supplement-1) ◽  
pp. 99-115 ◽  
Author(s):  
J. Beczner ◽  
I. Bata-Vidács

Vegetables and fruits are staple food for the human mankind, and they are also considered as the symbol of healthy nutrition. They are consumed fresh and cooked, in salad mixes, freshly pressed, fermented, minimally processed form, stored under different conditions, etc. Since they are in close contact with the environment, natural or artificial, and have a natural microbiota on their surface highly variable as a function of the surrounding, they are prone to get contaminated with human pathogens, too. More attention is paid to the food-borne outbreaks in the last 10 years related to the consumption of contaminated plant foods, and it is also in the focus of our interest. The main activities of the Unit cover the following areas: microbial contamination of fruits and vegetables, also in relation to the soil, the methods of cell count reduction using also non-thermal methods, the biofilm formation and the response ofBacillus cereusto the technological stresses.

Author(s):  
Kapil Telang ◽  
Rahul Jain ◽  
Ajoy Sodani ◽  
Prachi Shaw ◽  
Susmit Kosta

The current study was aimed to find out whether the COVID-19 virus is detectable upon the fruits and vegetables after coming in close contact with a patient suffering from nSARS-CoV-2. We included ten subjects, who tested positive for nSARS-CoV-2 RNA within seven days of the experiment. After explaining the experiment, a tray filled with seasonal vegetables and fruits were placed in front of them. The tray remained within their reach, for next thirty minutes. The subjects were requested to remove their face masks and remain so throughout the task. They were requested to manipulate the food articles the way they liked. Subjects were instructed to cough into their hands and then to manipulate each item at least 5 times, during the experiment. Thereafter, the trays were moved into an open and shaded area with free flow of natural air but no direct sunlight. After 1-hour, swabs were taken from surfaces of items by thoroughly rubbing over each of them. Samples were sent immediately to our RT-PCR lab. The nSARS-CoV-2 RNA was not detected, from the samples collected from the fruit/vegetable, at the end of one hour of the direct exposure to the COVID-19 patients. Our results suggest, even after direct exposure to and significant handling by the COVID-19 patients the nSARS-CoV-2 RNA remains undetected after one hour of storage in open. The fruits and vegetables, in real-life situations, are unlikely to act as a fomite and play any significant role in the spread of this disease.


Medicina ◽  
2009 ◽  
Vol 45 (4) ◽  
pp. 302
Author(s):  
Lina Škėmienė ◽  
Rūta Ustinavičienė ◽  
Ričardas Radišauskas ◽  
Jolita Kirvaitienė ◽  
Robertas Lažauskas ◽  
...  

Objective. To evaluate nutritional habits of middle-aged schoolchildren living in a town and district of Lithuania. Material and methods. The 8th–9th-grade schoolchildren were questioned by an anonymous questionnaire. A total of 329 students, 169 from Kaunas and 160 from Raseiniai district, participated in the study. Results. Only half of students reported that they had breakfast (61.3% of schoolboys and 47.6% of schoolgirls). More students from the district had dinner in the school canteen as compared to Kaunas students. Only 14.0% of schoolchildren followed nutrition regimen (14.8% of students from Kaunas and 13.1% of students from Raseiniai always had their meal at the same time). Every second schoolboy and schoolgirl consumed vegetables and fruits every day. Whole grain bread was consumed every day similarly frequently by students from Kaunas and Raseiniai, 25.4% and 18.8%, respectively; the students from Kaunas consumed skim milk more frequently, 26.0% and 11.9%, respectively (P<0.05). Confectionery was consumed more frequently by schoolchildren from Kaunas as compared to schoolchildren from Raseiniai district (63.9% vs. 55.0%); however, it was consumed more frequently by boys than girls. Less than one-fourth of Kaunas students (20.7%) consumed potato chips every day, whereas the percentage of the students from Raseiniai district was only 10.0% (P<0.05). Pizzas, kebabs, and hamburgers were consumed more frequently by schoolboys than schoolgirls. Knowledge of healthy nutrition was obtained mainly from media by 48.6% of students; every eighth student got this information from parents, every tenth from physicians, and 7.0% from teachers. Conclusions. Middle-aged students from both Kaunas town and Raseiniai district did not follow nutrition regimen; half of them did not have their breakfast before going to school. Fruits and vegetables as recommended foods were consumed by half of the participants; every fourth student consumed skim milk, and every fifth student consumed whole grain bread. The students more frequently consumed such foods as confectionery and smoked meat, which are not recommended (every second student); every fourth had an intake of carbonized drinks (the students from Kaunas more frequently) and potato chips (the students from Kaunas more frequently), and every tenth consumed pizzas and fried potatoes. More than half of students obtained knowledge of healthy nutrition from media, and every tenth – from the physicians.


Author(s):  
Le Bari Barine Gboeloh ◽  
Itoro Imaobong Sounyo

Parasitic contamination of fresh fruits and vegetables sold in selected markets in Port Harcourt Metropolis, Rivers State, Nigeria. was investigated. Oil mill and Creek Road were the two major markets in Port Harcourt metropolis area selected for this study. A total of 216 samples of different types of fruits and vegetables were randomly sampled for parasitological examination using normal saline and zinc sulphate floatation techniques. The results showed that out of the 216 samples of vegetables and fruits examined, 87 (40.3%) were infected. The results were statistically significant (P< 0.05). The parasites identified included Entamoeba coli; Entamoeba histolytica, Giardia lamblia, Balantidium coli, Ascaris lumbricoides, Ancyclostoma spp and Fasciola spp. Entamoeba coli was the most prevalent parasite (83.15%) while Ascaris lumbricodes (1.12%), Ancyclostoma spp (1.12%) and Fasciola spp (1.12%) had least prevalence. There was statistical no difference (P>0.05) in prevalence of parasites genus in the two markets. Tomatoes had the highest contamination rate of (10.6%) followed by waterleaf, bitter leaf, green leaf, pumpkin leaf, pear, orange, and scent leaf with the prevalence rate of 9.3%, 8.8%, 4.6%, 3.2%, 1.9%, 1.4% and 0.5% respectively. Produce contaminated with intestinal parasites poses a serious health challenge to the consumers if they are not properly washed before consumption. Personal hygiene and proper washing of fresh fruit and vegetable before consumption will reduce the prevalence of food-borne parasitic infections.


2014 ◽  
Vol 4 (2) ◽  
pp. 64-68
Author(s):  
Aleksandra Michalska

Abstract Nowadays, thanks to greater awareness of society and development of restorative medicine, more and more attention is paid to preventive care. That is caused by the fact that there is little progress for both sexes in the frequency of healthy behavior: girls fall much worse than boys in terms of frequency of physical activity, they do not eat breakfast either; boys do not maintain a healthy diet and are reluctant to eat vegetables and fruits, they often drink high-calorie sodas and less frequently brush teeth. Though with age some improvements in oral hygiene and certain eating behaviors can be noticed. It has been determined that overweight and obesity is a serious problem, as they can contribute to developmental disorders. In this respect it should be the responsibility of teachers to provide individual physical education (according to medical qualifications), prevent various forms of discrimination and bullying among peers, provide individual counseling and health education, weight control of students. It has been defined that for modern teachers it is a difficult task as students rarely eat fruits and vegetables, do not care about hygiene and frequency of meals, have passive mode of leisure. The acquisition of health during puberty allows functioning smoothly in society. However, despite the continuous work on improving and introduction of new programs of health education classes into schools of Poland, children still suffer from health-related problems. According to epidemiological research most of children in Poland fall on obesity, overweight and accompanying disorders and allergies. Youth is also exposed to accidents and related injuries. The problem is that students do not receive assistance and necessary information.


2018 ◽  
Vol 16 (1) ◽  
pp. 103-117
Author(s):  
Nurul Istiqomah ◽  
Nunung Sri Mulyani ◽  
Izza Mafruhah ◽  
Dewi Ismoyowati

Indonesia as an agricultural country has the potential to compete in the agricultural market in the international market, in line with the existence of the ASEAN / ASEAN Free Trade Area (AFTA) Free Market. Ngawi Regency is a fertile area and is one of the buffer zones of the agricultural sector in East Java. Horticulture commodities are one of the main sources in the agricultural sector, because they have high potential and can contribute to the economy of a region. Horticultural commodities in the form of fruits and vegetables are an important food source to meet the nutritional needs of the community. Agriculture with a focus on horticultural crops in Ngawi Regency was developed with a cluster system based on the level of progress, harvest area and by considering agro-climate to map the superior horticultural commodities. The purpose of this study was to map the conditions of horticultural agriculture and analyze problems in the cluster of horticulture plants in Ngawi Regency. The research method is a mixed method using descriptive analysis, Geographic Information System (GIS), and using the Analysis Hierarchy Process (AHP). The conclusion of this study is that the potential development of horticultural clusters in Ngawi Regency requires structuring and developing the location of base commodities in accordance with the conditions of the agro-ecosystem. The development of existing commodities at these base points will make the commodity superior and support the creation of horticultural cluster centers and the development of existing agribusiness in an area. Development of horticulture base commodities for seasonal vegetables and fruits can be adjusted to the LQ results for each sub-district in Ngawi Regency. The results of the Indepth interview processed using AHP obtained results that in fact there were three main factors in the development of clusters, namely production consisting of four derivative factors namely research and development, superior seeds, fertilizers and anti-pest drugs and then marketing with derivative factors namely product standardization, packaging , traditional markets and modern markets. Then the third factor of the institution consists of training, networking, government support and assistance. 


Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 60
Author(s):  
Vincenzo Michele Sellitto ◽  
Severino Zara ◽  
Fabio Fracchetti ◽  
Vittorio Capozzi ◽  
Tiziana Nardi

From a ‘farm to fork’ perspective, there are several phases in the production chain of fruits and vegetables in which undesired microbial contaminations can attack foodstuff. In managing these diseases, harvest is a crucial point for shifting the intervention criteria. While in preharvest, pest management consists of tailored agricultural practices, in postharvest, the contaminations are treated using specific (bio)technological approaches (physical, chemical, biological). Some issues connect the ‘pre’ and ‘post’, aligning some problems and possible solution. The colonisation of undesired microorganisms in preharvest can affect the postharvest quality, influencing crop production, yield and storage. Postharvest practices can ‘amplify’ the contamination, favouring microbial spread and provoking injures of the product, which can sustain microbial growth. In this context, microbial biocontrol is a biological strategy receiving increasing interest as sustainable innovation. Microbial-based biotools can find application both to control plant diseases and to reduce contaminations on the product, and therefore, can be considered biocontrol solutions in preharvest or in postharvest. Numerous microbial antagonists (fungi, yeasts and bacteria) can be used in the field and during storage, as reported by laboratory and industrial-scale studies. This review aims to examine the main microbial-based tools potentially representing sustainable bioprotective biotechnologies, focusing on the biotools that overtake the boundaries between pre- and postharvest applications protecting quality against microbial decay.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Monika Rusin ◽  
Joanna Domagalska ◽  
Danuta Rogala ◽  
Mehdi Razzaghi ◽  
Iwona Szymala

AbstractChemical contamination of foods pose a significant risk to consumers. A source of this risk is due to the consumption of products contaminated with heavy metals such as cadmium (Cd) and lead (Pb). The aim of the study was to research the levels of Cd and Pb contamination of selected species of vegetables and fruits in the form of fresh, frozen, dried and processed products. The goal was to verify which of these food groups was more contaminated with heavy metals. The study covered 370 samples of fruits and vegetables including apples, pears, grapes, raspberries, strawberries, cranberries, as well as beetroots, celeries, carrots and tomatoes. The content of Cd and Pb was determined by atomic absorption spectrometry. Quantitative results were analyzed using statistical models: analysis of variance, outlier analysis, post-hoc multiple comparison Tukey test. The tests showed that the levels of Cd and Pb concentration in samples of fresh, processed, frozen and dried fruits and vegetables varied substantially. The highest concentrations were recorded in dried products. Several fruit and vegetable samples exceeded the maximum permissible concentrations of Cd and Pb. The contamination of these products could be a significant source of consumer exposure to heavy metals when these products are a part of the diet.


2017 ◽  
Vol 8 (2) ◽  
pp. 134-145
Author(s):  
Wiena Arynda ◽  
Rosmida M Marbun

In Indonesia, 93.5% of the population aged ≥10 years still consume less fruits and vegetables 5 servings per day for 7 days a week (RISKESDAS, 2013).  The preliminary study conducted at SDN Mekarjaya I shows that the level of knowledge of school children about vegetables and fruits by 60% is still low and 93.3% of vegetable and fruit consumption is still lacking. In addition, SDN Mekarjaya I has never been used as a place of prior research. Therefore, it is necessary to conduct research on the knowledge of fruit vegetables in the fourth grade students of SDN Mekarjaya I. One of them is by doing counseling with the media of puzzle game. This research was conducted to know the increase of knowledge about vegetables and fruits before and after given counseling with puzzle game media in fourth grade students of SDN Mekarjaya I. This research was conducted by Pre-exsperiment method using "One group pretest and posttest" research design. Sampling by purposive sampling is 66 people. The statistical test used is paired sample t-test. Based on the results of the analysis shows that there is a significant difference of knowledge where p-value 0.000 or p <0,05 means there is a meaningful difference between before and after given counseling with puzzle game media.This puzzle game media can be developed by the teachers as a medium of learning in the classroom so that students are more motivated in following the learning process and make students do not feel bored


Author(s):  
Vladislav R. Kuchma ◽  
A. Yu. Makarova

Data of the hygienic assessment of the nutritional structure of students in the age periods that are critical for the formation of proper eating behavior is presened. The purpose of the study was to study the structure, nutrition regimen, dietary habits in the family, the nature of the physical activity of students. The questionnaire “Study of medical and social reasons for the formation of deviations in health and diseases in children” was used. 70-80% of students in Moscow schools have been established to be at risk in terms of lifestyle factors, among which a significant factor is a nutrition. The structure of their nutrition is characterized by insufficient consumption of biologically valuable food in 27-70% of children. The diet of 20-40% of respondents is depleted in the content of fermented milk products, vegetables, and fruits. Children exclude first courses (68-70%), breakfast cereals, fish, vegetables (35-40%), oils and eggs (29%) from their meals at an educational institution. Most schoolchildren outside educational institutions consume foods that are not intended for children food: chips, fast food, confectionery, sweet carbonated drinks.


2018 ◽  
Vol 14 (2) ◽  
pp. 172-177
Author(s):  
Joko Sudarsono ◽  
Setyo Sri Rahardjo ◽  
Kisrini Kisrini

The use of the pesticide which does not follow the procedure may potentially cause residue of pesticide to be left on vegetables and fruits sold in supermarkets and traditional markets. We aimed to find the pattern of pesticide usage and levels of residue found in plants. This study was an analytical observation with a cross-sectional design using detailed sampling procedure. We obtained the data of the pesticide residue concentration by conducting laboratory examinations on eight samples of cabbage and eight samples of tomatoes from traditional markets and supermarkets in the city of Solo. The data were tested using simple linear regression testing. The organophosphate measurement results showed no residues were detected, because it was below the Limit of Detection (LOD). We concluded that pesticides containing active substances were not used in the vegetables we tested or the active substances were no longer contained in the vegetables after harvesting.


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