Immunological parameters for assessing the sports abilities of football players

The creation of diagnostic tests to determine and monitor an athlete’s adaptation resources is a relevant task.Aim. To determine the levels of natural anti-glutamate receptor and anti-GABA antibodies in the dynamics of physical activity during training of football players. To establish the predictive role of these indicators in the objectification of adaptation.Material and methods. The experimental group involved 33 football players aged 18 to 24 years. The control group (n=33) consisted of volunteers of the same age engaged in fitness. Determination of immunological parameters was carried out by solid-phase ELISA.Results. It was found that each subject has an individual immunological profile, the parameters of which change depending on load received. The measured levels of anti-glutamate receptor and anti-GABA antibodies are consistent with the physical activity of a football player. According to the analysis results, the athletes were divided into three groups. The subjects of the first one had reliably high immunological indices and was at the peak of physical activity. Low values of the third group indicated a fatigue. The second group levels were within normal range and demonstrated resource capabilities for the load.Conclusion. The revealed immunological markers are suitable for the diagnostic and prognostic assessment of the athlete’s body functional state.

Download Full-text

New perspectives in immunological diagnostics of coronary heart disease

CARDIOVASCULAR THERAPY AND PREVENTION ◽

10.15829/1728-8800-2012-1-49-53 ◽

2012 ◽

Vol 11 (1) ◽

pp. 49-53

Author(s):

S. I. Davydov ◽

A. A. Tarasov ◽

A. L. Emelyanova ◽

M. A. Kiseleva ◽

A. R. Babaeva

Keyword(s):

Coronary Heart Disease ◽

Heart Disease ◽

Troponin I ◽

Solid Phase ◽

Diagnostic Methods ◽

Control Group ◽

Activity Measurement ◽

Immunological Parameters ◽

Coronary Syndrome ◽

Clinical Variants

Aim. To assess the diagnostic value of immunological markers of endothelial dysfunction (ED) in various clinical variants of coronary heart disease (CHD). Material and methods. The study included 455 patients with various clinical variants of CHD. The control group (CG) included 70 individuals without clinical CHD symptoms. Solid-phase immunoenzymatic method was used for measuring the serum titres of Chlamydia pneumoniae (Cp), sulphated glycosamineglycanes (s-GAG), collagen (C), and hyaluronic acid (HA) antibodies. Results. In patients with acute coronary syndrome (ACS), mean titres of Cp, s-GAG, C, and HA antibodies were significantly higher than in patients with chronic CHD or CG participants. Among patients with myocardial infarction (MI), the levels of troponin I (TrI) and MB-creatine phosphokinase (MB-CK) significantly correlated with the titres of s-GAG and C antibodies, while the levels of C-reactive protein (CRP) were linked to the titres of C and HA antibodies. In ACS with ST segment elevation, the measurement of Cp, s-GAG, C, and HA antibodies was comparable to the measurement of CRP, TrI, or MB-CK activity, being significantly more sensitive than echocardiography (EchoCG). In non-ST ACS, immunological parameters were as sensitive as electrocardiography or CRP measurement, more reliable than MB-CK activity measurement or EchoCG, and slightly less reliable than TrI measurement. In unstable angina pectoris (UAP), immunological analysis was significantly more sensitive than the majority of the standard diagnostic methods. Conclusion. Pathogenetic mechanisms of ACS are closely related to the development of specific autoimmune reactions. The assessed immunological parameters could be used as objective markers of acute CHD variants.

Download Full-text

Effects of 6 Weeks Direct Instruction and Teaching Games for Understanding Programs on Physical Activity and Tactical Behaviour in U-12 Soccer Players

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17145008 ◽

2020 ◽

Vol 17 (14) ◽

pp. 5008

Author(s):

Juan Vicente Sierra-Ríos ◽

Filipe Manuel Clemente ◽

Ezequiel Rey ◽

Sixto González-Víllora

Keyword(s):

Physical Activity ◽

Decision Making ◽

Direct Instruction ◽

Vigorous Physical Activity ◽

Soccer Players ◽

Control Group ◽

Football Players ◽

Evaluation Tool ◽

Teaching Games For Understanding ◽

Game Performance

The purpose of this study was to compare the effects of 6 weeks direct instruction and teaching games for understanding (TGfU) programs on the decision-making and execution (post-interventions), as well, as on the physical activity (PA) levels during sessions. Thirty under-12 football players participated in this study (age: 10.3 ± 0.45 years) and were randomly assigned to TGfU (n = 15) or direct instruction (n = 15) group. Two sessions/week were implemented. Results revealed that TGfU promoted higher levels (p = 0.043; d = 2.99) of light PA (28.96%) compared with direct instruction (27.55%). Non-significant higher sedentary PA levels (p = 0.073; d = 2.62) were found in the control group (35.48%). In terms of tactical principles, conservation of the ball increased the percentage of moderate to vigorous physical activity in TGfU (43.60%) compared with direct instruction (38.05%). According to the Game Performance Evaluation Tool (GPET), significant improvements (p = 0.018, d = 3.78) of the attacking player with the ball in the percentage of change between groups in the unsuccessful execution in TGfU (% = −62.2) were observed compared with direct instruction (% = 14.2). TGfU seems to be more appropriate than direct instruction to increase the light PA levels during sessions while no significant differences were found between programs in moderate and vigorous intensities. Regarding the effects of programs in decisions, greater improvements in decisions with the ball were found in TGFU compared to DI.

Download Full-text

EXAMINATION OF ISOKINETIC STRENGTH RATES OF KNEE JOINT (H/Q) IN FOOTBALL PLAYERS

JOURNAL OF SOCIAL SCIENCE RESEARCH ◽

10.24297/jssr.v10i4.4711 ◽

2016 ◽

Vol 10 (4) ◽

pp. 2248-2253

Author(s):

Ali Kerim YILMAZ ◽

Menderes KABADAYI ◽

M. Hakan MAYDA ◽

M. Ceyhun BÄ°RÄ°NCÄ°

Keyword(s):

Knee Joint ◽

Repeated Measures ◽

Personal Information ◽

Football Player ◽

Control Group ◽

Football Players ◽

Isokinetic Strength ◽

Significant Difference ◽

Angular Velocities ◽

And Control

The purpose of this study is to examine the isokinetic strength rates of knee joint (H/Q) in football players.30 males (15 football players, 15 controls) were included in the research who were studying in YaÅŸar DoÄŸu Sports Sciencies faculty of Ondokuz MayÄ±s University. The position, age and dominant leg information of the subjects were determined by filling in personal information forms given to the subjects. Body mass index measurements were recorded with a Gaia 359 plus body analyzer and H/Q force rates were measured and recorded with a Humac Norm Cybex Brand computer controlled isokinetic dynamometer at angles of 60osn-1, 180osn-1 and 240osn-1. For the statistical analysis of the data used SPSS 22.0 software was used. Independent t-test was applied for the analysis of football player-control group and dominant leg results. In addition, one-way variance analysis and LSD test were used for repeated measures to compare football players according to their positions. Statistical results were evaluated at 95% confidence interval and p <0.05 significance level.When we look at the descriptive information between the two groups in our study; There was a significant difference between age, body weight and sport ages, but no significant difference was observed in other parameters. When the H/Q power ratios at angular velocities of 60osn-1, 180osn-1 and 240osn-1 were examined, no significant difference was found between the dominant-nondominant force ratios and footballer positions between the two groups.As a result, when H/Q power ratios of footballer and control group are examined; There was no significant difference between the two groups when dominant-nondominant strength ratios and soccer player positions were compared.

Download Full-text

Soy milk and avocado juice: what is the best to reduce cholesterol among obesity children?

International Journal of Health Science and Technology ◽

10.31101/ijhst.v1i2.1098 ◽

2019 ◽

Vol 1 (2) ◽

Author(s):

Rizki Agustin Purwaningtyas ◽

Kustiningsih Kustiningsih

Keyword(s):

Physical Activity ◽

Cholesterol Level ◽

Male Student ◽

Control Group ◽

P Value ◽

Soy Milk ◽

High Cholesterol ◽

Central Java ◽

Equivalent Control ◽

Central Java Province

Children with obesity have high risk to have abnormal cholesterol rate. Obesity and high cholesterol rate can cause cardiovascular disease at a later time. Children have normal rate of cholesterol if the cholesterol rate in the blood is <170 mg/dL, the threshold category between 170-199 mg/dL, and high category is >200 mg/dL. Soy Milk and avocado juice are the ways of non pharmacological care that can be applied to reduce cholesterol rate. This study aims to compare Soy Milk and avocado juice giving toward cholesterol rate in children with obesity in State Elementary School 1 and 2 of Katerban, Central Java Province, Indonesia. The study used quasi experiment design with non-equivalent control group framework. Samples of the study were 30 children taken by use purposive sampling. Soy Milk and avocado juice effective to reduce cholesterol level in obesity children (p value=0,000, p<0,05), but neither soy milk and avocado juice there’re no one that more effective to decrease cholesterol level (p value=0,902, p>0,05). 60% of respondent were male student age 11 years (36,7%). Father education were high. Soya milk and avocado juice are able to reduce cholesterol rate. Parents must give attention to children’s dietary intake to reduce cholesterol and obesity, also motivate them to do physical activity.

Download Full-text

Impact of Physical Activity on the Incidence of Vascular Diseases in Adults with Type 2 Diabetes Mellitus

10.31661/gmj.v0i0.1549 ◽

2019 ◽

Vol 8 ◽

pp. 1549

Author(s):

Babak Pezeshki ◽

Ehsan Bahramali ◽

Amir Ansari ◽

Aliasghar Karimi ◽

Mojtaba Frajam ◽

...

Keyword(s):

Physical Activity ◽

Diabetes Mellitus ◽

Vascular Function ◽

Vascular Diseases ◽

Major Complication ◽

Control Group ◽

Case Group ◽

Vascular Events ◽

The Relationship

Background: Diabetes mellitus (DM) is a common metabolic disease worldwide and has many complications. The vascular events are the major complication of DM that have an important effect on mortality and disability. The physical activity (PA) enhances the vascular function by several pathways. The aim of this study was to evaluation of the relationship between PA and vascular diseases in patients with DM.Materials and Methods: This research was performed as the case-control study that was extracted from a prospective epidemiological research study in Iran (PERSIAN). The patients with type 2 DM more than six months defined as case group and the non-DM subjects in control group with ratio 1:2, and both groups were matched in the term of age and sex. The MET score was used to evaluate the level of PA and blood glucose, lipid profile, body mass index, overweight, dyslipidemia, glomerular filtration rate, myocardial infarction (MI), unstable angina, and stroke.Results: Overall, 1242 patients with DM were extracted, and 2484 non-diabetic subjects were investigated. In the case group, 355(28.6 %) and 887(71.4%) were men and women, respectively, the and 710 (28.6%) men and 1774(71.4%) women in control group. The mean MET score was 30 and 40.97 in the DM and non-DM groups, respectively (P˂0.001). The frequency of MI, stroke, and cardiac ischemia were 44 (3.5%), 37 (3%), and 267 (21.5%), respectively in DM group, and 54 (2.2%), 43 (1.7%), and 389 (15.7%), respectively in non-DM group.Conclusion: The incidence of vascular events associated with PA level in patients with DM and adherence to regular PA reduce the vascular events and DM complications. [GMJ.2019;inpress:e1549]

Download Full-text

ОЦЕНКА ЦИТОКИНОВОГО ПРОФИЛЯ СЫВОРОТКИ КРОВИ И СУБПОПУЛЯЦИЙ Т-ЛИМФОЦИТОВ У ДЕТЕЙ С АЛЛЕРГИЧЕСКИМИ ЗАБОЛЕВАНИЯМИ ОРГАНОВ ДЫХАНИЯ

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/raj.2019.3.40502 ◽

2019 ◽

pp. 52-60

Author(s):

E.V. Prosekova ◽

A.I. Turyanskaya ◽

N.G. Plekhova ◽

M.S. Dolgopolov ◽

V.A. Sabynych

Keyword(s):

Allergic Rhinitis ◽

Bronchial Asthma ◽

Allergic Inflammation ◽

Allergic Diseases ◽

Control Group ◽

Healthy Children ◽

Serum Cytokine ◽

Immunological Parameters ◽

Low Level ◽

Level Of Significance

Расширение спектра изучаемых клонов Тхелперов определило более сложные иммунные механизмы реализации аллергического воспаления. Цель. Характеристика показателей и взаимосвязей цитокинового профиля сыворотки и субпопуляционного состава Тлимфоцитов периферической крови у детей с бронхиальной астмой и аллергическим ринитом. Материалы и методы. Проведено комплексное обследование 150 детей в возрасте 311 лет с верифицированным диагнозом бронхиальной астмы, аллергического ринита и 30 здоровых сверстников. Иммунологические параметры крови оценивали методом проточной цитометрии, концентрации интерлейкинов и IgE в сыворотке крови определяли методом твердофазного иммуноферментного анализа. При статистической обработке использовали программы Statistica 10 с критическим уровнем значимости р0,05. Результаты. У детей с аллергическими заболеваниями в сыворотке крови определены высокие уровни содержания интерлейкинов4, 8, 13, 17А, сопоставимый с показателями группы контроля уровень IL17F и низкое содержание IFNy. При бронхиальной астме и аллергическом рините у детей выявлено увеличение количества CD3CD8CD45RO, CD3CD8CD45RACD45RO Тлимфоцитов и CD3CD4 Тхелперов и повышение количество Th17 при снижении CD3CD4CD45RO клеток памяти. В группе здоровых детей популяция Th17 составляла 9,491,6, у детей с аллергическими заболеваниями количество данных клеток было значимо выше 14,50,77 (р0,001). Анализ сывороточного содержания цитокинов у детей с изолированным течением БА и в сочетании с аллергическим ринитом выявил разнонаправленные корреляции, отличающиеся по силе и направленности от таковых в группе здоровых детей. Заключение. У детей при изолированном течении бронхиальной астмы и в сочетании с аллергическим ринитом выявлены: сопоставимое с показателями здоровых детей количество CD3CD4 Тклеток, дисбаланс в субпопуляционном составе Тхелперов за счет преобладания Th2 и Th17, активация синтеза IL17A, IL4, IL8, IL13, низкий уровень сывороточного IFNy, изменения силы и направленности взаимосвязей цитокинового профиля и спектра субпопуляций Тлимфоцитов.Expansion of the range of examined Thelper clones has determined more complex immune mechanisms for the implementation of allergic inflammation. Objective. To characterize the parameters and relationships between the serum cytokine profile and Tlymphocyte subpopulation in peripheral blood of children with bronchial asthma and allergic rhinitis. Materials and methods. 150 children aged between 311 years old with bronchial asthma, and allergic rhinitis and 30 healthy volunteers were examined. Immunological parameters were assessed by flow cytometry, the concentration of serum interleukins and IgE were determined by means of enzymelinked immunosorbent assay. Statistical analysis was performed with Statistica 10 program with a critical level of significance p0.05. Results. High levels of interleukins 4, 8, 13, 17A were determined, IL7F level was not significantly different from that in control group and low level of IFNy was found in the serum of children with allergic diseases. The number of CD3CD8CD45RO, CD3CD8CD45RACD45RO Tlymphocytes, CD3CD4 Thelper cells and Th17 were increased and at the same time CD3CD4CD45RO memory cells were decreased In bronchial asthma and allergic rhinitis children. Number of Th17 cells in healthy children was 9.491.6, in allergic children it was significantly higher 14.50.77 (p0.001). Analyses of serum cytokine count in children with isolated BA and in association with allergic rhinitis revealed multidirectional correlations differing in strength and direction from those in the group of healthy children. Conclusion. In children with isolated bronchial asthma and associated with allergic rhinitis the following parameters were found: CD3CD4 Tcells count was comparable to that in healthy children, the imbalance of Thelper subpopulation: prevalence of Th2 and Th17, activation of IL17A, IL4, IL8, IL13 synthesis and low level of serum IFNy.

Download Full-text

Effectiveness of Medication Review Services on Diabetes Self-Care in Primary Care Patients (Preprint)

10.2196/preprints.23269 ◽

2020 ◽

Author(s):

Tay Siew Cheng Sarah ◽

Lim Jit Fan Christina ◽

Tan Soo Chieng Daphne ◽

Tan Seok Yee Maureen ◽

Chen Jieying Cordelia ◽

...

Keyword(s):

Physical Activity ◽

Primary Healthcare ◽

Medication Review ◽

Intervention Group ◽

Self Care ◽

Effective Intervention ◽

Controlled Study ◽

Control Group ◽

Dietary Control ◽

Glucose Management

BACKGROUND Diabetes is one of the most common medical conditions referred to medication review service run by pharmacists, OBJECTIVE This study aims to determine whether pharmacist-run MRS can be an effective intervention tool to improve patients’ participation in self-care of diabetes. METHODS This randomised controlled study was conducted in five public primary healthcare centres from December 2014 to October 2016. Participants were 40 to 80 years of age and had been diagnosed with type 2 diabetes. These participants were prescribed with five or more chronic medications, of which at least one was an antidiabetic medication, by the primary healthcare centres’ doctors. The participants were randomly recruited into the intervention or control arm. A self-developed questionnaire which incorporated the validated Diabetes Self-Management Questionnaire (DSMQ) was administered face-to-face by the study team to the participants prior to and after MRS. MRS was not administered to participants in the control group. RESULTS A total of 221 participants completed the follow up. There were 105 participants in the control arm and 116 in the intervention arm. The DSMQ Sum Scale score of the control group improved by 0.16 ± 1.11 (p= 0.136) while the intervention group improved by 0.40 ± 0.99 (p=0.000). Participants in the intervention group reported a better improvement in their self-care of diabetes, specifically in glucose management (0.38± 1.35, p=0.003), dietary control (0.26±1.66, p=0.096) and physical activity (0.67±2.36, p=0.003). CONCLUSIONS Pharmacist-run MRS is an effective intervention tool to improve participants’ self-care of diabetes, particularly in glucose management, dietary control and physical activity.

Download Full-text

The clinical relevance of isolated lupus anticoagulant positivity in patients with thrombotic antiphospholipid syndrome

Thrombosis and Haemostasis ◽

10.1055/a-1344-4271 ◽

2020 ◽

Author(s):

Dong-mei Yin ◽

Philip de Groot ◽

Marisa Ninivaggi ◽

Katrien M.J. Devreese ◽

Bas de Laat

Keyword(s):

Antiphospholipid Syndrome ◽

Clinical Relevance ◽

Antiphospholipid Antibodies ◽

Odds Ratio ◽

Lupus Anticoagulant ◽

Solid Phase ◽

Control Group ◽

Domain I ◽

Normal Controls ◽

Better Than

Background: Patients positive for three types of antiphospholipid antibodies (aPLs) (triple positivity) have been identified at a high risk for thrombotic events. However, the clinical significance of isolated lupus anticoagulant (LAC) positivity is debated. Objectives: To investigate the clinical relevance of isolated LAC. Patients/Methods 456 patients were enrolled in this study; 66 antiphospholipid syndrome patients and 390 control patients. The control group existed of autoimmune patients (n=91), patients with thrombosis but without aPLs (n=127) and normal controls (n=172). The criteria LAC, anti-cardiolipin (anti-CL) and anti-beta2glycoprotein I (anti-β2GPI) IgG and IgM and the non-criteria IgA anti-CL and anti-β2GPI, anti-domain I (anti-DI) of β2GPI IgG and anti-phosphatidylserine/prothrombin (anti-PS/PT) IgG and IgM were detected according to the ISTH guidelines for solid phase assays. Results: 70 patients were positive for LAC, of which 44 were negative for both anti-β2GPI and anti-CL. We found that isolated LAC proved to be strongly associated with vascular thrombosis (Odds ratio (OR) (95% CI) 7.3 (3.3-16.1)), even better than triple positive samples (OR 4.3 (1.6-12.2)). The titers of the anti-PS/PT IgG and IgM were significantly higher in triple positivity samples compared to samples with isolated LAC positivity. The majority of single LAC positives were anti-PS/PT negative. We observed that LAC positivity was weaker in isolated LAC positive patients compared to LAC activity in triple positive patients. Conclusions: Isolated LAC was highly associated with thrombosis. The presence of anti-PS/PT could not explain LAC positivity in isolated LAC. Isolated LAC showed a weaker LAC activity compared to triple positive patients.

Download Full-text

Metabolic Evaluation of Urine from Patients Diagnosed with High Grade (HG) Bladder Cancer by SPME-LC-MS Method

Molecules ◽

10.3390/molecules26082194 ◽

2021 ◽

Vol 26 (8) ◽

pp. 2194

Author(s):

Kamil Łuczykowski ◽

Natalia Warmuzińska ◽

Sylwia Operacz ◽

Iga Stryjak ◽

Joanna Bogusiewicz ◽

...

Keyword(s):

Thin Film ◽

Bladder Cancer ◽

High Performance ◽

Biomarker Discovery ◽

Solid Phase ◽

Biological Fluids ◽

Reversed Phase ◽

Control Group ◽

Metabolic Evaluation ◽

Metabolomic Profiling

Bladder cancer (BC) is a common malignancy of the urinary system and a leading cause of death worldwide. In this work, untargeted metabolomic profiling of biological fluids is presented as a non-invasive tool for bladder cancer biomarker discovery as a first step towards developing superior methods for detection, treatment, and prevention well as to further our current understanding of this disease. In this study, urine samples from 24 healthy volunteers and 24 BC patients were subjected to metabolomic profiling using high throughput solid-phase microextraction (SPME) in thin-film format and reversed-phase high-performance liquid chromatography coupled with a Q Exactive Focus Orbitrap mass spectrometer. The chemometric analysis enabled the selection of metabolites contributing to the observed separation of BC patients from the control group. Relevant differences were demonstrated for phenylalanine metabolism compounds, i.e., benzoic acid, hippuric acid, and 4-hydroxycinnamic acid. Furthermore, compounds involved in the metabolism of histidine, beta-alanine, and glycerophospholipids were also identified. Thin-film SPME can be efficiently used as an alternative approach to other traditional urine sample preparation methods, demonstrating the SPME technique as a simple and efficient tool for urinary metabolomics research. Moreover, this study’s results may support a better understanding of bladder cancer development and progression mechanisms.

Download Full-text

Effectiveness of Text Messaging as an Incentive to Maintain Physical Activity after Cardiac Rehabilitation: A Randomized Controlled Pilot Study

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18126645 ◽

2021 ◽

Vol 18 (12) ◽

pp. 6645

Author(s):

Giulia Foccardi ◽

Marco Vecchiato ◽

Daniel Neunhaeuserer ◽

Michele Mezzaro ◽

Giulia Quinto ◽

...

Keyword(s):

Physical Activity ◽

Cardiac Rehabilitation ◽

Exercise Testing ◽

Text Messages ◽

Control Group ◽

Stand Test ◽

Randomized Controlled ◽

Sit To Stand ◽

Significant Difference

Although the efficacy of cardiac rehabilitation (CR) is proven, the need to improve patients’ adherence has emerged. There are only a few studies that have investigated the effect of sending text messages after a CR period to stimulate subjects’ ongoing engagement in regular physical activity (PA). A randomized controlled pilot trial was conducted after CR, sending a daily PA text message reminder to an intervention group (IG), which was compared with a usual care control group (CG) during three months of follow-up. Thirty-two subjects were assessed pre- and post-study intervention with GPAQ, submaximal iso-watt exercise testing, a 30 s sit-to-stand test, a bilateral arm curl test, and a final survey on a seven-point Likert scale. A statistically significant difference in the increase of moderate PA time (Δ 244.7 (95% CI 189.1, 300.4) minutes, p < 0.001) and in the reduction of sedentary behavior time (Δ −77.5 (95% CI 104.9, −50.1) minutes, p = 0.004) was shown when the IG was compared with the CG. This was associated with an improvement in heart rate, blood pressure, and patients’ Borg rating on the category ratio scale 10 (CR10) in iso-watt exercise testing (all p < 0.05). Furthermore, only the IG did not show a worsening of the strength parameters in the follow-up leading to a change of the 30 s sit-to-stand test with a difference of +2.2 (95% CI 1.23, 3.17) repetitions compared to CG (p = 0.03). The telemedical intervention has been appreciated by the IG, whose willingness to continue with regular PA emerged to be superior compared to the CG. Text messages are an effective and inexpensive adjuvant after phase 2 CR that improves adherence to regular PA. Further studies are needed to confirm these results in a larger patient population and in the long term.

Download Full-text