Mapping and Assessing Commercial Fisheries Services in the Lithuanian Part of the Curonian Lagoon

The spatial distribution of biomass of main commercial fish species was mapped to estimate the supply of a provisioning fishery service in the Curonian lagoon. Catch per unit effort (CPUE) was used as a proxy to estimate the efficiency of commercial fishing and, subsequently, the potential biomass of fishes. The relationship between distinctive characteristics of the fishing areas and corresponding commercial catches and CPUE was analyzed using multivariate analysis. The total catch values and CPUE used in the analyses were derived from the official commercial fishery records. RDE analysis was used to assess the variation of both catch and CPUE of commercial fish species, while the percentages of bottom sediment type coverage, average depth, annual salinity, and water residence time in each of the fishing squares were used as explanatory variables. This distance e-based redundancy analysis allowed for the use of non-Euclidean dissimilarity indices. Fisheries data spatial distribution map indicated the lack of coherence between the spatial patterns of commercial catches and CPUE distribution in the northern part of the lagoon. Highest CPUE values were estimated in the central-eastern part of the lagoon as compared to the western part of the lagoon where CPUE values were substantially lower. Both total catch and CPUE appeared not to be related to the type of bottom habitats statistically while being spatially correlated in-between. However, the impact of salinity and water residence time calculated using the 3D hydraulic circulation model on the distribution of both CPUE and commercial catches was statistically significant.

Biogeochemical Budgets of Nutrients and Metabolism in the Curonian Lagoon (South East Baltic Sea): Spatial and Temporal Variations

Estuaries are biogeochemical reactors able to modulate the transfer of energy and matter from the watershed to the coastal zones and to retain or remove large amounts of terrestrially generated nutrients. However, they may switch from nutrient sink to source depending upon interannual variability of the nutrient supply and internal processes driving whole system metabolism (e.g., net autotrophic or heterotrophic). We tested this hypothesis in the Curonian Lagoon, a hypertrophic estuary located in the south east Baltic Sea, following the budget approach developed in the Land-Ocean Interactions in the Coastal Zone (LOICZ) project. Annual budgets for nitrogen (N), phosphorus (P), and silica (Si) were calculated for the 2013–2015 period. The lagoon was divided in a flushed, nutrient loaded area, and in a confined, less loaded area. The lagoon was always a sink for dissolved inorganic Si and P whereas it was a N sink in the confined area, dominated by denitrification, and a N source in the flushed area, due to dinitrogen (N2) fixation. The net ecosystem metabolism (NEM) indicated that the Curonian Lagoon was mainly autotrophic because of high primary production rates. In this turbid system, low N:P ratio, high summer temperatures, and calm weather conditions support high production of N2-fixing cyanobacteria, suppressing the estuarine N-sink role.

Assessment of Four Major Fish Species Stocks in the Lithuanian and Russian Parts of Curonian Lagoon (SE Baltic Sea) Using CMSY Method

Fisheries in the Curonian Lagoon (1584 km2 coastal lagoon in South-east Baltic) can be classified as artisanal, small-scale, multi-species, and multi-gear. Such types of fisheries are characterised as data-poor and require appropriate investigation methods. We used CMSY (version CMSY_2019_9f. R) to assess maximum sustainable yield (MSY) and related indicators for freshwater bream, roach, pike-perch, and European perch. A decline in pike-perch and roach was identified, while the stocks of freshwater bream and European perch were sustained. As the CMSY model does not consider the impact of environmental factors, the decline in roach stock may be attributed to the increase in salinity rather than to overfishing. In the case of freshwater bream and pike-perch, the method cannot consider the increase of the percentage of small-sized fishes in catches due to the allowance of low-selectivity gears in the fishery. Additionally, in the case of the pike-perch, the model does not take into account the interannual fluctuations in the stock-recruitment system. The assessment of the European perch stock can be considered to be good. However, the accuracy of CMSY is limited, and it should be used for fisheries management only in combination with other methods.

Assessment of Ecosystem Services across the Land–Sea Interface in Baltic Case Studies

Spatial assessments of ecosystem services (ES) are needed to fulfil EU policy requirements and to support practical applications of the ES concept in policy implementation. So far, ES assessments have largely focused on terrestrial systems. A joint approach for land and sea is especially lacking. To overcome this gap, we present a novel spatial habitat typology and ES classification for an assessment across the land–sea interface. We build upon existing approaches and common spatial definitions, like CORINE land cover (CLC) types, water bodies of the Water Framework Directive (WFD), and habitat types according to the Habitats Directive (HD). We show applications of the resulting ES matrix for an expert-based assessment of ES potentials in three Baltic study sites (Schlei, Greifswald Bay and Curonian Lagoon). A complementary indicator-based approach to assess ES flows is introduced and applied. It enables a quantification of ES potentials and flows and ensures comparability among case study sites. Comparisons between the results for ES potentials and flows show that development capacities exist in particular for provisioning ES for marine habitats. Our approaches are spatially expandable and transferrable and could be applied to support environmental policy implementation. Further, we discuss their practical relevance, current limitations, and future research perspectives.

Characterization and Diversity of Microcystins Produced by Cyanobacteria from the Curonian Lagoon (SE Baltic Sea)

Microcystins (MCs) are the most widely distributed and structurally diverse cyanotoxins that can have significant health impacts on living organisms, including humans. The identification of MC variants and their quantification is very important for toxicological assessment. Within this study, we explored the diversity of MCs and their potential producers from the Curonian Lagoon. MC profiles were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) method, while the potential producers were detected based on the presence of genus-specific mcyE gene sequences. Among the numerous MCs detected, one new potential MC variant with m/z 1057 was partially characterized. Moreover, two other MCs with m/z 1075 and m/z 1068 might belong to new variants with serine (Ser), rarely detected in position one of the peptides. They might also represent MC-Y(OMe)R and MC-WR, respectively. However, the application of a low-resolution MS/MS system made the unambiguous identification of the MCs impossible. Based on this example, the problems of peptide structure identification are discussed in the work. Genetic analysis revealed that potential MCs producers include Dolichospermum/Anabaena, Microcystis spp., and Planktothrix agardhii. The diversity and temporal variations in MC profiles may indicate the presence of several chemotypes of cyanobacteria in the Curonian Lagoon.

Expert-Based Assessment and Mapping of Ecosystem Services Potential in the Nemunas Delta and Curonian Lagoon Region, Lithuania

The Curonian Lagoon (SE Baltic Sea) and the coastal wetland system play a vital role in maintaining the overall cultural, economic and ecological health of the region. However, its value is still largely underestimated and there is a lack of integrated ecosystem management based on multiple ecosystem services assessment. In this study, 34 ecosystem services (ES) were identified, assessed and mapped following the Common International Classification of Ecosystem Services (CICES). Using expert opinion, 35 land cover classes were scored for their ES potential, these being 15 CORINE land cover classes (CLC) and 20 Natura 2000 areas of conservation (habitat types, i.e., Sites of Community Importance). The total ES potential was calculated by averaging the opinions of experts, using self-confidence scoring as weights, and the ES relative importance index was derived from a questionnaire. As a result, three maps corresponding to each ES potential category (provisioning, regulation and maintenance and cultural) were calculated and further used for hot- and coldspot analyses. Regulation and maintenance and cultural ES were highly interrelated and had the highest potential in the studied area. Forested areas had the highest total ES potential and contributed significantly to the land cover of the ES hotspots.

Phytoplankton of the Curonian Lagoon as a New Interesting Source for Bioactive Natural Products. Special Impact on Cyanobacterial Metabolites

The bioprospecting of marine and brackish water systems has increased during the last decades. In this respect, microalgae, including cyanobacteria, and their metabolites are one of the most widely explored resources. Most of the bioactive compounds are isolated from ex situ cultures of microorganisms; however, analysis of field samples could also supply valuable information about the metabolic and biotechnological potential of microalgae communities. In this work, the activity of phytoplankton samples from the Curonian Lagoon was studied. The samples were active against antibiotic resistant clinical and environmental bacterial strains as well as against serine proteases and T47D human breast adenocarcinoma cells. No significant effect was found on Daphnia magna. In addition, using LC-MS/MS, we documented the diversity of metabolites present in field samples. A list of 117 detected cyanopeptides was presented. Cyanopeptolins constituted the largest class of cyanopeptides. As complex bloom samples were analyzed, no link between the observed activity and a specific sample component can be established. However, the results of the study showed a biotechnological potential of natural products from the Curonian Lagoon.

Spatial and Temporal Diversity of Cyanometabolites in the Eutrophic Curonian Lagoon (SE Baltic Sea)

This work aims to determine the profiles of cyanopeptides and anatoxin synthetized by cyanobacteria in the Lithuanian part of the Curonian Lagoon (SE Baltic Sea) and to characterize their spatial and temporal patterns in this ecosystem. Cyanometabolites were analysed by a LC-MS/MS system and were coupled to a hybrid triple quadrupole/linear ion trap mass spectrometer. During the investigation period (2013–2017), 10 microcystins, nodularin, anatoxin-a, 16 anabaenopeptins, including 1 oscillamide, 12 aeruginosins, 1 aeruginosamide, 3 cyanopeptolins and 4 microginins were detected. The most frequently detected metabolites were found at all investigated sites. Demethylated microcystin variants and anabaenopeptins had the strongest relationship with Planktothrix agardhii, while non-demethylated microcystin variants and anatoxin had the strongest relationship with Microcystis spp. Low concentrations of some microcystins: [Asp3]MC-RR, MC-RR, MC-LR, as well as a few other cyanopeptides: AP-A and AEG-A were found during the cold period (December–March). Over the study period, Aphanizomenon, Planktothrix and Microcystis were the main dominant cyanobacteria species, while Planktothrix, Microcystis, and Dolichospermum were potentially producers of cyanopeptides and anatoxin detected in samples from the Curonian Lagoon.

The second stage of development of artificial reproduction technology of northern pike in the Kaliningrad lagoon basin

The second stage of development of northern pike artificial reproduction technology for stocking with larvae and fry in the Kaliningrad lagoon basin is considered in this paper. Today northern pike natural stocks in the Kaliningrad lagoon basin are not numerous due to unregulated fishery and limited spawning habitat. Since the 1990s, in many countries of the world, the number of natural stocking northern pike has gradually decreased and at present does not exceed several thousand tons. This problem is also relevant for the Baltic Sea basin, where the modern catches of northern pike in the Curonian and Kaliningrad lagoons make 7,2 and 0,6 tons respectively. At the present time different scientific programs and actions are realized in many countries to restore natural stocks of northern pike. One of the most promising solutions of low northern pike numbers problem is organization of northern pike artificial reproduction in the territory of the Kaliningrad Region. Based on our earlier research in the Curonian lagoon basin, the second stage of development of the technology of northern pike artificial reproduction was carried out on the basis of the educational and experimental farm of KSTU in 2020. Prespawning maintenance of 25 northern pike producers with the use of cages from deli, ponds and reclamation canal was carried out, as well as egg incubation and holding of prelarvae in RAS. As a result of the conducted researches, it was established, that the reproductive products of northern pike producers have high quality and the prelarvae at the stage of incubation have high survival rate (82,2–91,7%). The calculated of biometric parameters of producers and their reproductive products, confirmed the correlation between the weight of females and their fecundity, which was 0,86 and 0,95. The scheme of keeping of producers and incubation of northern pike eggs tested at the second stage has shown the prospect of providing 50% of the need in stocking of young northern pike in the Kaliningrad lagoon with the use of 100% stock of producers.

Social Dimensions of Projected Climate Change Impacts on Ecosystem Services in the Coastal-Rural Area of Nemunas River Reaches and Curonian Lagoon (Lithuania)

The capacity of ecosystems to provide ecosystem services (hereinafter referred to as ES) depends on the state of their structure, processes, and functions, which is determined by interactions with other systems. These interactions are complex and take place in different climatic areas, and have different impacts on ecosystems and the use of ES. As the most sensitive part of the Lithuanian shore zone to climate change is the seaside zone or the coastal zone, which includes the Curonian Lagoon and the sea coast, the said area was therefore chosen for research. The case study examined those ES that were specific to the study area, without attempting to account for absolutely all ES. With an emphasis on the representation of different perspectives (in the process of assessing and preserving the potential of ES), the empirical study involved representatives of different (public and private) sectors. The public sector was represented by elders and eldership employees, the private sector by farmers and businesspeople. Respondents were selected purposefully to reflect the entire existing totality of the existing area. The evaluation of the obtained theoretical and practical results of the research identified the potential of the existing area ecosystem services and perspectives for the implementation of services by assessing their use according to respondents’ opinions, regional climate change, and national scale in EU environmental policy.