scholarly journals Water uptake dynamics in soybean seeds: influence in seeds performance and DNA integrity

2021 ◽  
Vol 51 (3) ◽  
Author(s):  
Lisiane Sartori Pereira ◽  
Tathiana Elisa Masetto
Keyword(s):  
Water Uptake ◽  
Uptake Rate ◽  
Dna Isolation ◽  
Critical Role ◽  
Soybean Seed ◽  
Dna Integrity ◽  
Soybean Seeds ◽  
Water Control ◽  
Degraded Samples ◽  
High Germination

ABSTRACT: Relationships among water uptake rate and temperatures were investigated in five soybean seeds lots cv. M 6410 IPRO. Germination, field emergence and electrical conductivity tests were applied to determine seed performance after fast (complete immersion in water), control (moist substrate) and slow (moist atmosphere) imbibitions both at 20 °C and 30 °C. The fresh weight increment over time in soybean seeds during imbibition was recorded. In parallel, analysis of the DNA integrity of seedlings obtained from such seeds was performed in gel electrophoresis. Absorption pattern of soybean seeds were similar among temperatures, but absorption characteristics vary with time progress according to the way of water entrance into the seeds. Upon fast imbibition seeds germinated lower than non-fast-imbibed seeds, besides loss of seed performance. In contrast, slow-imbibed seeds showed high germination, low abnormal seedlings and maintenance of seeds performance. DNA isolation from fast imbibed seeds was highly degraded; although, some considerably degraded samples were reported in controlled imbibed seeds and the best preserved DNA was found in slow-imbibed seeds. The pattern of DNA degradation is typically passive or non programmed cell death. Our results showed it is important to consider the water uptake rate during germination test, since DNA integrity plays a critical role during seed imbibition, preserving soybean seed performance.

scholarly journals Effect of Sowing Date on Germination and Vigour of Soybean (Glycine max (L.) Merr) Seeds

2013 ◽  
Vol 11 (1) ◽  
pp. 67-75 ◽  
Author(s):  
MM Rahman ◽  
MM Rahman ◽  
MM Hossain
Keyword(s):  
Soybean Seed ◽  
Sowing Date ◽  
Soybean Seeds ◽  
Rabi Season ◽  
Sowing Dates ◽  
High Germination ◽  
Physiological Quality ◽  
Glycine Max L ◽  
Plot Design

An experiment was carried out at the Agronomy Field Laboratory of Bangladesh Agricultural University, Mymensingh during three consecutive seasons in 2004-2006 to find out the effect of sowing date on physiological quality of soybean seed. The experiments included six sowing dates viz. 4 Nov, 24 Nov, 14 Dec, 3 Jan, 23 Jan and 12 Feb and two cultivars namely G-2 and PB-1 in Rabi 2004-05; four sowing dates viz. 25 Jul, 14 Aug, 3 Sept and 23 Sept and three cultivars namely G-2, PB-1 and BS-5 in Kharif II 2005; four sowing dates viz. 26 Nov, 16 Dec, 5 Jan, and 25 Jan, and two cultivars viz. G-2 and PB-1 in Rabi 2005-06. The experiments were conducted in split-plot design with three replications. Results revealed that cultivar G-2 had higher germination and vigour than cultivar PB-1 or BS-5 in all the three seasons. November and December sowing produced seeds with higher germination and vigour for all the cultivars in Rabi season. On the other hand, September sowing produced seeds with high germination and vigour during Kharif II. The study indicated that soybean seeds with high germination and vigour could be obtained by sowing during November to December in Rabi season and August to September in Kharif-II season. DOI: http://dx.doi.org/10.3329/agric.v11i1.15245 The Agriculturists 2013; 11(1) 67-75

scholarly journals Genetic diversity in soybean seed quality under different storage conditions

2017 ◽  
Vol 38 (1) ◽  
pp. 57
Author(s):  
Daniele Piano Rosa ◽  
Danúbia Aparecida Costa Nobre ◽  
Diego Santos Oliveira ◽  
Francisco Charles dos Santos Silva ◽  
André Ricardo Gomes Bezerra ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Quality ◽  
Soybean Seed ◽  
High Standard ◽  
Storage Conditions ◽  
Seed Vigor ◽  
Soybean Cultivars ◽  
High Germination ◽  
Physiological Quality ◽  
Almost All

This study aimed to assess the effect of genetic diversity on physiological quality of soybean seeds stored in cold chamber and under environmental conditions. Ten cultivars were assessed in a randomized factorial design (2x10). Factor 1 corresponded to two storage conditions and factor 2 to ten soybean cultivars, with four replications. The evaluated variables were total germination (G%), first count of germination (F%), percentage of abnormal seedlings (AS%), germination speed index (GSI), water content (WC), electrical conductivity (EC), dry matter of seedlings (DMS) and length of seedlings (LS). Data underwent ANOVA, followed by Scott Knott test, as well as multivariate analysis of genetic diversity. The results showed a higher physiological quality for seeds under cold storage. Half of the cultivars (FPS Júpiter, FPS Urano, FPS Antares, FPS Netuno and CD 250) presented high germination rates and seed vigor, being thus indicated as high-standard materials for further breeding programs. Besides that, storage environment had influence on the clustering of soybean cultivars. Moreover, cultivars had genetic dissimilarity for almost all assessed traits as G%, GSI, F%, AS%, EC, DMS and LS.

scholarly journals Exogenous Abscisic Acid Application Effects on Stomatal Closure, Water Use, and Shelf Life of Hydrangea (Hydrangea macrophylla)

2009 ◽  
Vol 27 (4) ◽  
pp. 234-238 ◽  
Author(s):  
Marc W. van Iersel ◽  
Kate Seader ◽  
Sue Dove
Keyword(s):  
Abscisic Acid ◽  
Shelf Life ◽  
Water Uptake ◽  
Stomatal Closure ◽  
Ornamental Plants ◽  
Detectable Effect ◽  
Water Control ◽  
Available Water ◽  
Hydrangea Macrophylla ◽  
Plant Available Water

Abstract A lack of adequate watering reduces the shelf life of many ornamental plants during retail. Our goals were to determine whether sprays or drenches with abscisic acid (ABA) can reduce transpiration and extend the shelf life of hydrangea (Hydrangea macrophylla). During the first 5 days after treatment, ABA drenches of 125 to 1000 ppm reduced stomatal conductance (gs) by 50 to 80% as compared to water. ABA-induced stomatal closure reduced plant water uptake from the substrate; control plants took up half of the plant-available water during the first 7 days after treatment, while it took 14 days for plants drenched with 1000 ppm to take up half of the available water. Control plants wilted after 12 days and time to wilting of drenched plants increased with increasing ABA concentrations, up to 23 days in the 1000 ppm treatment. Spray treatments had little effect on gs and no detectable effect on water uptake or time to wilting. Some yellowing of older leaves was seen with ABA drenches of 500 or 1000 ppm. Despite this side effect, ABA drenches have potential to extend the shelf life of hydrangeas in retail environments.

scholarly journals Experimental evaluation of organic substrates for germination testing of soybean seed

2020 ◽  
Vol 26 (2) ◽  
pp. 1-6
Author(s):  
Marija Milivojević ◽  
Dragana Branković-Radojčić ◽  
Radmila Vukadinović ◽  
Jasna Kojić ◽  
Zoran Dumanović ◽  
...  
Keyword(s):  
Soybean Seed ◽  
Laboratory Method ◽  
Alternative Methods ◽  
Routine Practice ◽  
Organic Substrates ◽  
Growing Media ◽  
Positive Effects ◽  
Temperature Regimes ◽  
High Germination ◽  
Crop Density

High germination of seeds and seedling establishment are critical for soybean production since it determines crop density and eventually affects the yield. Accurate evaluation of the germination potential of sowing material is valuable, as well as initial quality assessment of newly harvested seed. ISTA Rules prescribe alternative methods for soybean seed germination: sand, between paper, top of paper covered with sand and since 2020 organic growing media. In the Seed Testing Laboratory at the Maize Research Institute soybean germination is conducted in the sand at 20<=>30°C. The objective of this study was to evaluate several available organic growing media and compare results obtained with the standard laboratory method. Five soybean seed lots of different quality were tested in four germination growing media (3 organic and sand), at two temperature regimes (25 °C and 20<=>30°C). Statistical analyses showed that all types of organic growing media were of the same quality. Slightly higher germination in organic growing media was obtained in the experiment at 20<=>30°C compared to sand, but those differences were not statistically significant. When testing was done in germinator with 25°C positive effects of organic growing media were identified and this impact was significant, especially for low-quality seed lots. The experimental outcome was to continue with routine practice and use organic growing media for retesting of seed lots with lower germination as an alternative substrate.

scholarly journals Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

2021 ◽  
Vol 43 ◽  
Author(s):  
Thiago Barbosa Batista ◽  
Samara Moreira Perissato ◽  
Carlos Henrique Queiroz Rego ◽  
Gustavo Roberto Fonseca de Oliveira ◽  
Fernando Augusto Henning ◽  
...  
Keyword(s):  
Electrical Conductivity ◽  
Linear Regression ◽  
Soybean Seed ◽  
Accelerated Aging ◽  
Storage Period ◽  
High Potential ◽  
Seed Longevity ◽  
Soybean Seeds ◽  
Radicle Protrusion ◽  
Over Time

Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.

scholarly journals Necroptotic Cell Death Signaling and Execution Pathway: Lessons from Knockout Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-15 ◽  
Author(s):  
José Belizário ◽  
Luiz Vieira-Cordeiro ◽  
Sylvia Enns
Keyword(s):  
Cell Death ◽  
Knockout Mice ◽  
Critical Role ◽  
Stress Conditions ◽  
Dna Integrity ◽  
Caspase 8 ◽  
Necrotic Cell Death ◽  
Necrotic Cell ◽  
Injury Death ◽  
Regulated Necrosis

Under stress conditions, cells in living tissue die by apoptosis or necrosis depending on the activation of the key molecules within a dying cell that either transduce cell survival or death signals that actively destroy the sentenced cell. Multiple extracellular (pH, heat, oxidants, and detergents) or intracellular (DNA damage and Ca2+overload) stress conditions trigger various types of the nuclear, endoplasmic reticulum (ER), cytoplasmatic, and mitochondrion-centered signaling events that allow cells to preserve the DNA integrity, protein folding, energetic, ionic and redox homeostasis, thus escaping from injury. Along the transition from reversible to irreversible injury, death signaling is highly heterogeneous and damaged cells may engage autophagy, apoptotic, or necrotic cell death programs. Studies on multiple double- and triple- knockout mice identifiedcaspase-8,flip, andfaddgenes as key regulators of embryonic lethality and inflammation. Caspase-8 has a critical role in pro- and antinecrotic signaling pathways leading to the activation of receptor interacting protein kinase 1 (RIPK1), RIPK3, and the mixed kinase domain-like (MLKL) for a convergent execution pathway of necroptosis or regulated necrosis. Here we outline the recent discoveries into how the necrotic cell death execution pathway is engaged in many physiological and pathological outcome based on genetic analysis of knockout mice.

scholarly journals Near-infrared spectroscopy used to predict soybean seed germination and vigour

2018 ◽  
Vol 28 (3) ◽  
pp. 245-252 ◽  
Author(s):  
Maythem Al-Amery ◽  
Robert L. Geneve ◽  
Mauricio F. Sanches ◽  
Paul R. Armstrong ◽  
Elizabeth B. Maghirang ◽  
...  
Keyword(s):  
Infrared Spectroscopy ◽  
Seed Germination ◽  
Near Infrared Spectroscopy ◽  
Near Infrared ◽  
Prediction Models ◽  
Soybean Seed ◽  
Seed Vigour ◽  
Near Infrared Spectra ◽  
High Germination ◽  
Qualitative Models

AbstractRapid, non-destructive methods for measuring seed germination and vigour are valuable. Standard germination and seed vigour were determined using 81 soybean seed lots. From these data, seed lots were separated into high and low germinating seed lots as well as high, medium and low vigour seed lots. Near-infrared spectra (950–1650 nm) were collected for training and validation samples for each seed category and used to create partial least squares (PLS) prediction models. For both germination and vigour, qualitative models provided better discrimination of high and low performing seed lots compared with quantitative models. The qualitative germination prediction models correctly identified low and high germination seed lots with an accuracy between 85.7 and 89.7%. For seed vigour, qualitative predictions for the 3-category (low, medium and high vigour) models could not adequately separate high and medium vigour seeds. However, the 2-category (low, medium plus high vigour) prediction models could correctly identify low vigour seed lots between 80 and 100% and the medium plus high vigour seed lots between 96.3 and 96.6%. To our knowledge, the current study is the first to provide near-infrared spectroscopy (NIRS)-based predictive models using agronomically meaningful cut-offs for standard germination and vigour on a commercial scale using over 80 seed lots.

No Effect of Soybean Lipoxygenase on Aflatoxin Production in Aspergillus flavus–Inoculated Seeds

2002 ◽  
Vol 65 (12) ◽  
pp. 1984-1987 ◽  
Author(s):  
J. E. MELLON ◽  
P. J. COTTY
Keyword(s):  
Aspergillus Flavus ◽  
Fungal Pathogens ◽  
Seed Viability ◽  
Soybean Seed ◽  
Fungal Growth ◽  
Aflatoxin Production ◽  
Aflatoxin Contamination ◽  
Soybean Seeds ◽  
Heat Treated ◽  
Significant Difference

Soybean lines lacking lipoxygenase (LOX) activity were compared with soybean lines having LOX activity for the ability to support growth and aflatoxin B1 production by the fungal seed pathogen Aspergillus flavus. Whole seeds, broken seeds, and heat-treated (autoclaved) whole seeds were compared. Broken seeds, irrespective of LOX presence, supported excellent fungal growth and the highest aflatoxin levels. Autoclaved whole seeds, with or without LOX, produced good fungal growth and aflatoxin levels approaching those of broken seeds. Whole soybean seeds supported sparse fungal growth and relatively low aflatoxin levels. There was no significant difference in aflatoxin production between whole soybean seeds either with or without LOX, although there did seem to be differences among the cultivars tested. The heat treatment eliminated LOX activity (in LOX+ lines), yet aflatoxin levels did not change substantially from the broken seed treatment. Broken soybean seeds possessed LOX activity (in LOX+ lines) and yet yielded the highest aflatoxin levels. The presence of active LOX did not seem to play the determinant role in the susceptibility of soybean seeds to fungal pathogens. Seed coat integrity and seed viability seem to be more important characteristics in soybean seed resistance to aflatoxin contamination. Soybean seeds lacking LOX seem safe from the threat of increased seed pathogen susceptibility.

scholarly journals Adhesion of uredospores of Phakopsora pachyrhizi on soybean seeds and their viability during storage

2012 ◽  
Vol 34 (2) ◽  
pp. 225-230
Author(s):  
Elisandra Batista Zambenedetti Magnani ◽  
Elisabeth Aparecida Furtado de Mendonça ◽  
Maria Cristina de Figueiredo e Albuquerque
Keyword(s):  
Soybean Seed ◽  
Germination Percentage ◽  
Time Span ◽  
Soybean Seeds ◽  
Phakopsora Pachyrhizi ◽  
Mato Grosso ◽  
Soybean Production ◽  
Soybean Plants ◽  
Production Areas

To study adhesion and viability of uredospores of the fungus Phakopsora pachyrhizi on soybean seeds during storage, suspension tests of those uredospores were carried out by washing seeds at each 30 days interval. Furthermore, germination and inoculation tests of uredospores on soybean plants were performed with uredospores collected from seeds of two soybean production areas, located in the municipalities "Chapada dos Guimarães" and "Tangará da Serra", State of Mato Grosso, Brazil. High levels of uredospores infestation were detected before storage [249.31 and 85.18 uredospores/100 seeds (U/100)] on seeds collected in both localities, respectively. After 30 days storage, these figures were 46.12 and 122.5 U/100; at 60 days were 14.62 and 26.62 U/100; and at 90 days were only 2.87 and 3,68 U/100, respectively; dropping to zero after 120 days storage. The percentage of germinated uredospores decreased with increasing storage periods and at 120 days germination percentage was nil. When uredospores were inoculated on soybean plants, rust symptoms were only observed for uredospores collected from freshly harvested seeds. Uredospores associated to soybean seed germinate until 90 days after storage, but are not viable after this time span. Infection of plants only occurs with inoculation of uredospores obtained from freshly harvested seeds.

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