scholarly journals Development of periradicular lesions in normal and diabetic rats

2006 ◽  
Vol 14 (5) ◽  
pp. 371-375 ◽  
Author(s):  
Luci Armada-Dias ◽  
Jorge Breda ◽  
José Claudio Provenzano ◽  
Marisa Breitenbach ◽  
Isabela das Neves Rôças ◽  
...  
Keyword(s):  
Histopathological Examination ◽  
Experimental Period ◽  
Diabetic Rats ◽  
Microbial Pathogens ◽  
Control Group ◽  
Diabetic Patients ◽  
Histopathological Analysis ◽  
Radiographic Analysis ◽  
Radiographic Images ◽  
Group Data

Evidence suggests that diabetic patients are more significantly affected by problems of endodontic origin. This study sought to radiographically and histologically examine the development of periradicular inflammation in control and in diabetic rats after induction of pulpal infection. The pulps of the mandibular first molars of normal and streptozotocin-induced diabetic rats were exposed and left in contact with their oral cavities for 21 and 40 days. Afterwards, the animals were sacrificed, the mandibles were surgically removed, fixed in formalin and then radiographed in a standardized position. The radiographic images of the periradicular lesions were scanned and computerized images were evaluated for the total area of the lesions using a specific software. Representative specimens were also prepared for histopathological analysis. Radiographic analysis revealed that diabetic rats presented significantly larger periradicular lesions when compared with control rats, regardless of the experimental period (p<0.05). Histopathological examination of representative specimens revealed larger periradicular lesions and more severe inflammatory exudate in the group of diabetic rats when compared with the control group. Data from the present study indicated that diabetic rats can be more prone to develop large periradicular lesions, possibly due to reduction in the defense ability against microbial pathogens.

scholarly journals Effect of Terebinthus atlanticus on Glucose Metabolism in Diabetic Rats

2020 ◽  
Vol 20 (1) ◽  
pp. 31-40
Author(s):  
Fadwa El-Ouady ◽  
Lhoussaine Hajji ◽  
Mohamed Eddouks
Keyword(s):  
Blood Glucose ◽  
Oral Administration ◽  
Aqueous Extract ◽  
Antioxidant Activities ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Control Group ◽  
Histopathological Analysis ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background:: Terebinthus atlanticus (Anacardiaceae) is an important source of essential oil and phenolic compounds justifying its use in traditional medicine. Objective:: The present work aimed to evaluate the antidiabetic and the antioxidant activities of the aqueous extract of the leaves of Terebinthus atlanticus (T. atlanticus). Methods:: The current study evaluated the effect of a single and repeated (15 days of treatment) oral administration of the aqueous extract of the leaves of T. atlanticus (PALAE) on blood glucose levels in normal and streptozotocin(STZ)-induced diabetic rats. Furthermore, the effect of PALAE on glucose tolerance and histopathological examination of the liver was carried out. Results:: A single oral administration of PALAE reduced blood glucose levels in normal (p<0.05), and STZ diabetic rats (p<0.0001), 6 and 4 hours after administration, respectively. Furthermore, this extract had an optimal effect (p<0.0001) in both normal and STZ diabetic rats at the 15th and 7th day of treatment. This extract was also shown to prevent significantly the increase on blood glucose levels 120 min after glucose administration, in both normal (p<0.05), and diabetic (p<0.01) treated rats when compared to the control group. In addition, the histopathological analysis highlighted the positive effect of T. atlanticus on pancreas and liver. Conclusion:: The study demonstrates the antihyperglycemic effect of the aqueous T. atlanticus extracts in diabetic rats which should be mediated through the amelioration of the oxidative stress as well as an improvement in liver histology.

scholarly journals RUTIN RESTORE BIOCHEMICAL CHANGES, OXIDATIVE STRESS AND BETATROPHIN LEVEL IN STZ-INDUCED DIABETIC RATS

2019 ◽  
pp. 62-70
Author(s):  
AL-SHIMAA M. ABAS ◽  
AHMED F. EL-FARAFG ◽  
NEHAL W. ABDALLA
Keyword(s):  
Oxidative Stress ◽  
Histopathological Examination ◽  
Experimental Period ◽  
Positive Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Albino Rats ◽  
Standard Group ◽  
Group V ◽  
Group I

Objective: Diabetes mellitus (DM) is associated with long-term damage, dysfunction, of various organs. Study aims to assessrole of rutin on experimentally induced diabetes. Methods: 50 adult male albino rats divided into 5 groups. Group I (control group, rats were orally administered with 1 ml saline daily). Group II (DMSO group, rats were orally administered with 0.2 % DMSO for 60 d orally). Group III (positive control, animals were injected intraperitoneally with 60 mg/kg b. wtstreptozotocin followed by intraperitoneal injection with 120 mg/kg b. wt of Nicotinamide after 15 min). Group IV (therapeutic group, diabetic rats treated with 100 mg/kg b. wt of rutin for 60 d orally). Group V (standard group, diabetic animals treated with 100 mg/kg b. wt of metformin for 60 d orally). At the end of the experimental period blood serum and plasma, liver, kidney and pancreatic tissues were collected. Results: Diabetic rats showed a significant increase in plasma glucose, serum urea, creatinine, cholesterol and triglyceride. Also, induced oxidative stress as pointed out an increase in MDA level, decrease in GSH level, GST and CAT activities in compared to control group. Also, showed an increase in plasma and tissues levels of betatrophin. Oral administration of rutin cause decrease in elevated biochemical and oxidative stress parameters. Also, decrease betatrophin level when compared with diabetic rats. Our results were confirmed by histopathological examination of different tissues. Conclusion: This study suggests thatrutinexihibitsantihyperglycemic and antioxidant activity in streptozotocin-induced diabetic rats.

scholarly journals Effect of alpha-lipoic acid on antioxidant gene expression and kidney injury in alloxan-induced diabetic rats

2018 ◽  
Vol 8 (1) ◽  
pp. 6-6 ◽  
Author(s):  
Parisa Jamor ◽  
Hassan Ahmadvand ◽  
Hesam Ashoory ◽  
Esmaeel Babaeenezhad
Keyword(s):  
Gene Expression ◽  
Lipoic Acid ◽  
Kidney Injury ◽  
Diabetic Rats ◽  
Pcr Analysis ◽  
Control Group ◽  
Diabetic Patients ◽  
Alpha Lipoic Acid ◽  
The Impact ◽  
Gpx Activity

Background: Myeloperoxidase (MPO) is involved in the initiation, progression, and complications of atherosclerosis in diabetic patients. Objectives: In the current study, the impact of alpha-lipoic acid (LA), a natural antioxidant and a cofactor in the enzyme complexes on MPO, catalase (CAT) and glutathione peroxidase (GPx) activity, glutathione (GSH) and malondialdehyde (MDA) level, histopathology of kidney and expression of antioxidant enzymes, superoxide dismutase (SOD), GPx and CAT which are involved in the detoxification of reactive oxygen species (ROS), was evaluated in alloxan-induced diabetic rats. Materials and Methods: In this study, 30 male Rattus norvegicus rats randomly divided into three groups; control (C), non-treated diabetic (NTD), and LA-treated diabetics (LATD) was induced by alloxan monohydrate (100mg/kg; subcutaneous [SC]). Then treatment was performed with alphaLA (100 mg/kg intraperitoneal (i.p) daily to 6 weeks). Blood sample of animals collected to measure levels of MPO, CAT and GPx activity GSH and MDA. Kidney paraffin sections were prepared to estimate histological studies and to measure quantitative gene expression SOD, GPX and CAT in kidney. Results: Induction of diabetes led to a significant increase in MPO and MDA, reduced GSH level and GPx and CAT activities (P < 0.05). However, treatment with alpha-LA led to a significant elevation in GPx, CAT and GSH levels with a reduction in MPO activities and MDA levels (P < 0.05). Furthermore, the real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis results showed increased expressions of GPx, CAT and SOD enzyme in the treatment group compared with the diabetic control group. Histopathological lesions such as increased glomerular volume and lymphocyte infiltration were attenuated in the alpha-LA treated group. Conclusions: Our findings indicated that alpha-LA supplementation is effective in preventing complications induced by oxidative stress and atherosclerosis in diabetic rats.

scholarly journals Hypoglicemic and Hypolipedimic Effects of Ganoderma lucidum in Streptozotocin-Induced Diabetic Rats

Medicines ◽  
2018 ◽  
Vol 5 (3) ◽  
pp. 78 ◽  
Author(s):  
Erna Bach ◽  
Edgar Hi ◽  
Ana Martins ◽  
Paloma Nascimento ◽  
Nilsa Wadt
Keyword(s):  
Ganoderma Lucidum ◽  
Biochemical Analysis ◽  
Food Supplement ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Control Group ◽  
Histopathological Analysis ◽  
Beta Glucan ◽  
Male Wistar Rats ◽  
Normoglycemic Control

Background:Ganoderma lucidum (Leyss. Ex. Fr) Karst is a basidiomycete mushroom that has been used for many years as a food supplement and medicine. In Brazil, National Health Surveillance Agency (ANVISA) classified Ganoderma lucidum as a nutraceutical product. The objective of the present work was to observe the effects of an extract from Ganoderma lucidum in rats treated with streptozotocin, and an agent that induces diabetes. Method: Male Wistar rats were obtained from the animal lodging facilities of both University Nove de Julho (UNINOVE) and Lusiada Universitary Center (UNILUS) with approval from the Ethics Committee for Animal Research. Animals were separated into groups: (1) C: Normoglycemic control water; (2) CE: Normoglycemic control group that received hydroethanolic extract (GWA); (3) DM1 + GWA: Diabetic group that received extract GWA; and (4) DM1: Diabetic group that received water. The treatment was evaluated over a 30-day period. Food and water were weighted, and blood plasma biochemical analysis performed. Results: G. lucidum extract contained beta-glucan, proteins and phenols. Biochemical analysis indicated a decrease of plasma glycemic and lipid levels in DM rats induced with streptozotocin and treated with GWA extract. Histopathological analysis from pancreas of GWA-treated DM animals showed preservation of up to 50% of pancreatic islet total area when compared to the DM control group. In plasma, Kyn was present in diabetic rats, while in treated diabetic rats more Trp was detected. Conclusion: Evaluation from G. lucidum extract in STZ-hyperglycemic rats indicated that the extract possesses hypoglycemic and hypolipidemic activities. Support: Proj. CNPq 474681/201.

The potential protective effect of α-lipoic acid against nanocopper particle–induced hepatotoxicity in male rats

2016 ◽  
Vol 36 (9) ◽  
pp. 881-891 ◽  
Author(s):  
AA Khalaf ◽  
AR Zaki ◽  
MK Galal ◽  
HA Ogaly ◽  
MA Ibrahim ◽  
...  
Keyword(s):  
Lipoic Acid ◽  
Copper Content ◽  
Experimental Period ◽  
Liver Function Tests ◽  
Male Rats ◽  
Control Group ◽  
Histopathological Analysis ◽  
Tissue Samples ◽  
Group I ◽  
Apoptotic Genes

The present research task is aimed to evaluate the role of exogenous α-lipoic acid (ALA) (100 mg/kg body weight) as hepatoprotective and potent antioxidant in amelioration of copper nanoparticle (CNP)-induced hepatotoxicity. Forty male rats were randomly assigned into four equal groups: group I (control), group II received CNPs, group III received CNPs + ALA, and finally group IV received ALA for 2 months. At the end of the experimental period, the rats were decapitated, and blood and liver tissue samples were collected for measurement of liver function tests, antioxidant status, lipid peroxidation (LPO), copper content, expression of some apoptotic genes, and histopathological analysis. CNPs induced marked hepatic damages as evident by severe alteration in hepatic biomarkers. This was accompanied by a significant elevation in hepatic LPO and induced nitric oxide, copper content, and expression level of apoptotic genes (C-myc and C-jun). In contrast, marked depletion for antioxidant parameters was detected. These findings were confirmed with severe pathological alterations. Coadministration of ALA as a powerful antioxidant attenuates the hepatotoxic effects of CNPs through improvement of liver parameters, oxidative status, genetic changes, and preservation of liver integrity through histopathological analysis. These results suggest that consumed ALA could be used as an applicable hepatoprotective agent against oxidative damage mediated by nanoparticles intoxication.

scholarly journals Effect of Vitex Agnus-Castus Ethanolic Extract on Sex Hormones in Streptozotocin-Induced Diabetic Rats

2020 ◽  
Author(s):  
Fatemeh Soleymanzadeh ◽  
Minoo Mahmoodi ◽  
Siamak Shahidi
Keyword(s):  
Sex Hormones ◽  
Experimental Studies ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Female Rats ◽  
Control Group ◽  
Diabetic Patients ◽  
High Dose ◽  
Fruit Extract ◽  
Agnus Castus

Objective: Diabetes mellitus is recognized as one of the serious global health problems. There are evidences regarding the high prevalence of sexual dysfunction in diabetic patients. Experimental studies revealed a positive effect of Vitex agnus-castus (Vitex), on sexual function and behaviors. In this research, the effect of Vitex on sexual hormones in streptozotocin-(STZ) induced diabetic rats was investigated. Materials and methods: A Thirty adult female Wistar rats were divided into five groups. 1-control group (non-diabetic), 2- diabetic group (received normal saline) and three induced diabetic groups treated with different doses (400, 200 and 100 mg/kg) of Vitex. Treatment groups received Vitex fruit extract by gavage for 7 days. The levels of luteinizing hormone (LH), follicle-stimulating hormone (FSH), progesterone and estrogen in serum were measured. Results: Levels of LH, FSH, estrogen and progesterone and average body weight was lower in diabetic group compared to control group (p <0.010). Animals received high dose of Vitex fruit extract (400mg/kg) had significantly higher levels of serum LH, FSH, estrogen and progesterone compared to diabetic group (p < 0.010). In animals receiving minimum dose (100mg/kg) of Vitex, no difference was observed compared to diabetic group (p > 0.010). Conclusion: It can be concluded that Vitex fruit extract probably has regulatory effect on diabetes-induced change in the levels of sex hormones in female rats. Vitex fruit extract can improve serum levels of sex hormones in an animal model of STZ-induced diabetes.

Metformin protects against diabetes–induced heart injury and dunning prostate cancer model

2020 ◽  
pp. 096032712094745
Author(s):  
BB Bayrak ◽  
P Koroglu ◽  
O Karabulut Bulan ◽  
R Yanardag
Keyword(s):  
Prostate Cancer ◽  
Tissue Damage ◽  
Total Antioxidant Status ◽  
Experimental Period ◽  
Heart Tissue ◽  
Diabetic Rats ◽  
Control Group ◽  
Cancer Group ◽  
Total Antioxidant

In this study, both diabetes and Dunning prostate cancer were induced for the first time in Copenhagen rats in vivo. Thus, the effects of metformin against heart tissue damage of these rats were investigated by biochemical methods. Dunning prostate cancer was induced in Copenhagen rats using high metastatic MAT-LyLu cells. The rats were divided as follows: Control group: only injected with 0.9% NaCl for 14 days; Diabetic group: only injected single dose of streptozotocin (STZ) (65 mg/kg); Cancer group: subcutaneously (s.c) inoculated with 2 x 104 MAT-LyLu cells only; Diabetic + cancer (DC) group: inoculated with 2 x 104 MAT-LyLu cells and STZ injection, Cancer + metformin (CM) group: injected with metformin for 14 days after Mat-LyLu cells application; Diabetic + cancer + metformin (DCM) group: metformin administered for 14 days together with STZ and Mat-LyLu cells. At the end of the experimental period, heart tissues were taken. Reduced glutathione and total antioxidant status levels in heart tissues were decreased, whereas lipid peroxidation, advanced oxidized protein products, nitric oxide, homocysteine, and reactive oxygen species levels, total oxidant status and catalase, superoxide dismutase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase and xanthine oxidase activities increased in the diabetic, cancer and DC groups. Treatment with metformin reversed these effects. In conclusion, the present study shows that metformin has a protective effect against heart tissue damage in STZ-induced diabetic rats with Dunning prostate cancer.

Decreased RhoA expression in myocardium of diabetic rats

2005 ◽  
Vol 83 (8-9) ◽  
pp. 775-783 ◽  
Author(s):  
Jiping Tang ◽  
Sharyn M Fitzgerald ◽  
Brandi N Boughtman ◽  
Samuel W Cole ◽  
Michael W Brands ◽  
...  
Keyword(s):  
Diabetic Rats ◽  
Small Gtpase ◽  
Control Group ◽  
Diabetic Patients ◽  
Sprague Dawley ◽  
Total Rna ◽  
Sprague Dawley Rats ◽  
Diabetic Myocardium ◽  
Rhoa Protein ◽  
Membrane Bound

Diabetic cardiomyopathy is 1 of the major causes of death in diabetic patients, but the pathogenesis is unclear. There is evidence that RhoA, a small GTPase, might be involved in cardiac function. This study, therefore, analyzed RhoA expression and activation in hearts of diabetic rats. Male Sprague–Dawley rats were divided into control and diabetic groups of 18 each. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). Rats were studied 3 weeks after induction of diabetes. Heart rate, which was measured 24 h/day, decreased by 93 ± 7 beats/min in diabetic rats. There was a 62% decrease (p < 0.01) in RhoA mRNA expression in heart tissues (left ventricle) of diabetic rats (38.5 ± 6.7 × 106 molecules/µg total RNA) compared with controls (101 ± 10.3 × 106 molecules/µg total RNA). Western blot showed a 33% decrease in total RhoA protein expression in heart tissues of diabetic rats compared with controls (p < 0.05). A reduced RhoA translocation in heart tissues of diabetic rats was determined by a 64% decrease in membrane-bound RhoA (p < 0.01 vs. control group), indicating that the activation of RhoA is markedly reduced in diabetic myocardium. Our data suggest that down-regulated RhoA may be involved in cardiomyopathy in diabetic rats.Key words: RhoA, diabetes, heart.

scholarly journals Thymoquinone Lowers Blood Glucose and Reduces Oxidative Stress in a Rat Model of Diabetes

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2348
Author(s):  
Mohamed Faisal Lutfi ◽  
Abdel-Moneim Hafez Abdel-Moneim ◽  
Ashwag Saleh Alsharidah ◽  
Mugahid A. Mobark ◽  
Ahmed A. H. Abdellatif ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Renal Function ◽  
Glycaemic Control ◽  
Histopathological Examination ◽  
Glycated Haemoglobin ◽  
Diabetic Rats ◽  
Control Group ◽  
Group Iii ◽  
Group I ◽  
Lipid Peroxidase

The aim of the present study was to assess the short-term effects of Thymoquinone (TQ) on oxidative stress, glycaemic control, and renal functions in diabetic rats. DM was induced in groups II and III with a single dose of streptozotocin (STZ), while group I received no medication (control). The rats in groups I and II were then given distilled water, while the rats in group III were given TQ at a dose of 50 mg/kg body weight/day for 4 weeks. Lipid peroxidase, nitric oxide (NO), total antioxidant capacity (TAC), glycated haemoglobin (HbA1c), lipid profiles, and renal function were assessed. Moreover, the renal tissues were used for histopathological examination. STZ increased the levels of HbA1c, lipid peroxidase, NO, and creatinine in STZ-induced diabetic rats in comparison to control rats. TAC was lower in STZ-induced diabetic rats than in the control group. Furthermore, rats treated with TQ exhibited significantly lower levels of HbA1c, lipid peroxidase, and NO than did untreated diabetic rats. TAC was higher in diabetic rats treated with TQ than in untreated diabetic rats. The histopathological results showed that treatment with TQ greatly attenuated the effect of STZ-induced diabetic nephropathy. TQ effectively adjusts glycaemic control and reduces oxidative stress in STZ-induced diabetic rats without significant damaging effects on the renal function.

scholarly journals Evaluation of Sclerorhachis leptoclada hydroalcoholic extract on glucose and cholesterol levels, triglyceride, Serum HDL and LDL in male diabetic rats

2020 ◽  
pp. 344-354
Keyword(s):  
Group Treatment ◽  
Density Lipoprotein ◽  
Serum Levels ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
The Body ◽  
Control Group ◽  
Antioxidant Compounds ◽  
Diabetic Patients ◽  
Hydroalcoholic Extract

Background and Aim: Diabetes is a metabolic disorder in the body. Sclerorhachis leptoclada belongs to the chicory family, is used to treat aids and cancer due to the antioxidant compounds in the root. Therefore, in this study, the effect of hydroalcoholic extract of Sclerorhachis leptoclada on glucose and cholesterol, triglyceride, high density lipoprotein (HDL) and low density lipoprotein (LDL) levels in diabetic rats was investigated. Materials and Methods: In this experimental study, 60 male Wistar rats were randomly divided into 10 groups of 6: Control group, treatment groups 1, 2, and 3 (healthy, treated with 150 mg/kg and 300 and 600 extracts), Diabetic control group, experimental groups 1, 2 and 3 (diabetic, treated with doses of 150 mg/kg and 300 and 600 extracts), Positive control group (diabetic+glibenclamide), The healthy group (healthy+glibenclamide) was divided. After one month of gavage, blood samples were taken from the rats, and glucose, urea, creatinine, and albumin levels were measured. A comparison was made between the effect of hydroalcoholic extract of Sclerorhachis leptoclada and the common drug glibenclamide and the results of the groups were compared by using t-test and ANOVA. Results: The results showed that the administration of hydroalcoholic extract of Sclerorhachis leptoclada can significantly reduce glucose levels in experimental groups (diabetic+extract at doses of 150, 300, 600) compared with the diabetic group (p<0.001) Also, the consumption of hydroalcoholic extract of Sclerorhachis leptoclada in diabetic patients causes a significant reduction in triglyceride, HDL and LDL levels in diabetic rats and has hypoglycemic effects. Conclusion: The results of this study showed that the consumption of hydroalcoholic extract of Sclerorhachis leptoclada in diabetic rats significantly reduces serum levels of glucose, triglyceride and creatinine in diabetic rats and has hypoglycemic effects

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