scholarly journals Detection of sulfamethazine in water, milk and pig manure by dipstick immunoassay

2008 ◽  
Vol 52 (No. 10) ◽  
pp. 445-450 ◽  
Author(s):  
V.B. Kandimalla ◽  
N. Kandimalla ◽  
K. Hruska ◽  
M. Franek
Keyword(s):  
Cost Effective ◽  
Food Samples ◽  
Pig Manure ◽  
Screening Methods ◽  
Test Results ◽  
Visual Measurement ◽  
The Past ◽  
Colour Development ◽  
Control Spot ◽  
Simple Screening

During the past few years, there has been an increasing interest in rapid visual tests that could be performed outside the laboratory, for example on farms, in store houses or in food production plants. Hence, cost effective and simple screening methods are required for residual analysis of environmental and food samples on-site. Here, a simple and instrumental independent dipstick immunoassay for sulfamethazine detection is described. The polyclonal antibody was optimised in terms of coating dilution on a nitrocellulose membrane, dilution of peroxidase tracer conjugate, blocking agents and incubation times. Test results assessed by visual measurement can be available within 20 minutes. In buffer, water, skimmed milk and pig manure extract, sulfamethazine fortified at 50 and 100 µg/l has exhibited clear visual differentiation in colour development (lower intensity) in comparison to the control spot intensity (high intensity) of the dipstick.

scholarly journals Comparison of Visual Immunoassay and Chromogenic Culture Medium for the Presence of Listeria spp. in Foods

2002 ◽  
Vol 85 (5) ◽  
pp. 1201-1204 ◽  
Author(s):  
Philip Istafanos ◽  
Lawrence James
Keyword(s):  
Culture Medium ◽  
Food Samples ◽  
Screening Tools ◽  
Rapid Screening ◽  
Screening Methods ◽  
Visual Test ◽  
Test Results ◽  
Early Screening ◽  
Agar Method ◽  
Excellent Tool

Abstract Two rapid screening methods [the TECRA™ Listeria Visual Immunoassay (LIS–VIS) kit, an AOAC-approved 48 h visual test, which detects Listeria through colorimetry, and BCM™ Listeria isolation and differentiation plating agar] were used to screen U.S. Food and Drug Administration-regulated commodities for the presence of Listeria spp. Seventy-four different food samples were screened for the presence of Listeria spp. by using both protocols. Test results for the TECRA LIS–VIA showed 66 negative samples and 1 false positive, with 4 confirmed as L. monocytogenes and 3 as L. innocua. With the BCM agar, 67 samples were negative, 4 were confirmed as L. monocytogenes, and 3 were confirmed as L. innocua. Both methods showed similar results and were effective screening tools for Listeria spp. in foods. The BCM agar method proved to be a rapid, sensitive, and excellent tool for early screening and differentiation of Listeria spp. present in foods.

Efficiency of Two Commercial ELISA Kits Compared with the BAM Culture Method for Detecting Listeria in Naturally Contaminated Foods

1991 ◽  
Vol 74 (5) ◽  
pp. 819-821 ◽  
Author(s):  
Charles W Noah ◽  
Nora C Ramos ◽  
Virginia M Gipson
Keyword(s):  
Culture Method ◽  
Elisa Test ◽  
Food Samples ◽  
Alternative Methods ◽  
Rapid Screening ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Methods ◽  
Test Results ◽  
Enrichment Broth ◽  
University Of Vermont

Abstract The efficiency of 2 commercial enzyme-linked Immunosorbent assay (ELISA) kite (Listeria-Tek™ and Tecra™) for detecting Listeria in naturally contaminated foods was evaluated and compared with that of the culture method described in the Bacteriological Analytical Manual (BAM). Both ELISAs use modified University of Vermont (UVM-1) medium as a primary enrichment; the BAM method uses Listeria enrichment broth. Secondary enrichments for Llsterla-Tek and Tecra, respectively, were Fraser broth and UVM-2, which contains additional acriflavln-HCI. When ELISA test results differed, secondary enrichments were tested against the other ELISA; Fraser broth was used to determine recovery rates because of Its superiority over UVM-2. Of the 178 food samples examined, the presence of Listeria was detected and culturally confirmed in 38, 37, and 40 samples by the BAM, Llsterla- Tek, and Tecra methods, respectively. Differences in results of the EUSAs compared with those of the BAM method were not statistically significant; however, differences between results of the 2 ELISA methods were significant. It was concluded that as rapid screening methods, the Llsteria-Tek and the Tecra kits qualify as alternative methods to the BAM cultural method.

scholarly journals COST-EFFECTIVE MODEL OF SCREENING FOR TYPE 2 DIABETES IN THE REPUBLIC OF UZBEKISTAN

2021 ◽  
Vol 14 (3) ◽  
Author(s):  
A Alimov ◽  
F Khaidarova ◽  
S Ismailov ◽  
G Rakhimova ◽  
D Nazhmutdinova ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Primary Care ◽  
Healthy Lifestyle ◽  
Cost Effective ◽  
Epidemiological Studies ◽  
Economic Problem ◽  
Screening Methods ◽  
Test Results ◽  
The Republic

Epidemiological studies revealed up to 5-6 undiagnosed cases of type 2 diabetes mellitus (DM2) per 1 registered patient. The late diagnosis of DM2 presents a medical and socio-economic problem. Aim. To identify the most cost-effective, easy-to-use method for regular screening for DM2 in primary care settings in the Republic of Uzbekistan. Material and methods. 4 diabetes screening scenarios differing in the inclusion criteria (age 45 vs age 45 plus arterial hypertension plus obesity), and screening methods (testing for glycemia and/or HbA1c) were studied in urban and rural primary care polyclinics among 2430 people. Results. In primary health care real clinical practice, the most cost-effective and easy-to-follow strategy for active screening for DM2 is testing for random glycemia using a certified glucometer, in any resident aged 45 or older visiting his/her GP for any reason. If test results are intermediate, the person is called again the next day for fasting glycemia testing to confirm the diagnosis. If the test results are normal, the person is given recommendations of healthy lifestyle and re-testing in 1 year. If the test result corresponds to DM, the person is referred to a local endocrinologist.

Hemoglobin Profiles and Hematologic Features of Thalassemic Newborns: Application to Screening of α-Thalassemia 1 and Hemoglobin E

2008 ◽  
Vol 132 (11) ◽  
pp. 1739-1745
Author(s):  
Jaruwan Tritipsombut ◽  
Kanokwan Sanchaisuriya ◽  
Supan Fucharoen ◽  
Goonnapa Fucharoen ◽  
Nirut Siriratmanawong ◽  
...  
Keyword(s):  
Mean Corpuscular Volume ◽  
Cost Effective ◽  
Screening Methods ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Volume ◽  
Corpuscular Hemoglobin ◽  
Hemoglobin E ◽  
Polymerase Chain ◽  
Blood Specimens ◽  
Simple Screening

Abstract Context.—Thalassemia and hemoglobinopathies are major public health problems worldwide. To establish a cost-effective screening tool for newborns in regions where the incidence of these disorders is significant, study of the hemoglobin and hematologic features of normal and thalassemic newborns is necessary. Objective.—To study hemoglobin and hematologic characteristics of normal and various thalassemic newborns and to assess the effectiveness of simple screening methods for α-thalassemia 1 and hemoglobin E. Design.—Study was made of 402 cord blood specimens collected from unrelated Thai individuals. Hematologic parameters and hemoglobin profiles were determined. Thalassemia mutations were identified using polymerase chain reaction–related techniques. Results.—As many as 178 subjects (44.3%) were found to carry thalassemia genes with 18 different genotypes. All forms of α-thalassemia including double heterozygote for hemoglobin E and α-thalassemia showed significant reduction in hemoglobin, mean corpuscular volume, and mean corpuscular hemoglobin with increasing trend of red blood cell as compared with a non–α-thalassemic group. Although heterozygous hemoglobin E and β-thalassemia showed no hematologic difference from nonthalassemic group, heterozygous α-thalassemia 1 including those with hemoglobin E showed significant increase in hemoglobin Bart level. Conclusions.—Based on these findings, effective primary screening with 100% accuracy for α-thalassemia 1 and hemoglobin E in newborns in the region could be carried out using mean corpuscular volume less than 95 fL, mean corpuscular hemoglobin less than 30 pg, or hemoglobin Bart greater than 8.0% and hemoglobin E greater than 0.5%, respectively.

scholarly journals Calculations of compressed rods considering experiments over last 100 years

2021 ◽  
Vol 263 ◽  
pp. 02049
Author(s):  
Denis Konin
Keyword(s):  
Bearing Capacity ◽  
Ultimate Load ◽  
Compressive Load ◽  
Cost Effective ◽  
Test Results ◽  
Fe Modelling ◽  
The Past ◽  
Wide Range ◽  
Line Diagram ◽  
Design Cost

The article presents the results of testing and FE-modelling of rods calculated for the central and eccentric compressive load. More than 600 tests have been analysed over the past 100 years, including made by author, in a wide range of slenderness made of steels with a yield strength of up to 1000 MPa. It is established that the existing Russian calculation methods allow us to accurately determine the bearing capacity of the rod for steel of any strength. Verification of calculation method was carried out not only by stresses and ultimate load-bearing capacity, but also by the deformations of the tested rods. It is established that for H-beams and thin- walled pipes, clarification of the design codes is required to be able to design cost-effective designs, since the code formulas give a margin. This is especially true for elements of low flexibility with small eccentricities. To clarify the codes, a method for modelling a three-line diagram of steel operation, verified with the test results, is proposed.

Regulatory and sustainability initiatives lead to improved polyaminopolyamide epichlorohydrin (PAE) wet-strength resins and paper products

TAPPI Journal ◽  
2018 ◽  
Vol 17 (09) ◽  
pp. 519-532 ◽  
Author(s):  
Mark Crisp ◽  
Richard Riehle
Keyword(s):  
Health And Safety ◽  
Cost Effective ◽  
Worker Safety ◽  
Regulatory Requirements ◽  
Wet Strength ◽  
The Past ◽  
Consumer Safety ◽  
Federal Institute ◽  
Sustainability Initiatives ◽  
The Impact

Polyaminopolyamide-epichlorohydrin (PAE) resins are the predominant commercial products used to manufacture wet-strengthened paper products for grades requiring wet-strength permanence. Since their development in the late 1950s, the first generation (G1) resins have proven to be one of the most cost-effective technologies available to provide wet strength to paper. Throughout the past three decades, regulatory directives and sustainability initiatives from various organizations have driven the development of cleaner and safer PAE resins and paper products. Early efforts in this area focused on improving worker safety and reducing the impact of PAE resins on the environment. These efforts led to the development of resins containing significantly reduced levels of 1,3-dichloro-2-propanol (1,3-DCP) and 3-monochloropropane-1,2-diol (3-MCPD), potentially carcinogenic byproducts formed during the manufacturing process of PAE resins. As the levels of these byproducts decreased, the environmental, health, and safety (EH&S) profile of PAE resins and paper products improved. Recent initiatives from major retailers are focusing on product ingredient transparency and quality, thus encouraging the development of safer product formulations while maintaining performance. PAE resin research over the past 20 years has been directed toward regulatory requirements to improve consumer safety and minimize exposure to potentially carcinogenic materials found in various paper products. One of the best known regulatory requirements is the recommendations of the German Federal Institute for Risk Assessment (BfR), which defines the levels of 1,3-DCP and 3-MCPD that can be extracted by water from various food contact grades of paper. These criteria led to the development of third generation (G3) products that contain very low levels of 1,3-DCP (typically <10 parts per million in the as-received/delivered resin). This paper outlines the PAE resin chemical contributors to adsorbable organic halogens and 3-MCPD in paper and provides recommendations for the use of each PAE resin product generation (G1, G1.5, G2, G2.5, and G3).

Urban pollution control strategy: SBR technology to treat agricultural waste from pig farm in Indonesia

2019 ◽  
Vol 2 (2) ◽  
pp. 21
Author(s):  
Lindawati Lindawati
Keyword(s):  
Cost Estimation ◽  
Batch Reactor ◽  
Agricultural Waste ◽  
Cost Effective ◽  
Land Application ◽  
Pig Manure ◽  
Small Scale ◽  
Piggery Wastewater ◽  
Pig Farm ◽  
Number Of Customers

Reduction of food rations and shortages is one of the impacts of the increasing human population. Food sector industries then try to cope with the fast growing number of customers. Agribusiness sector gains its popularity in these recent years, including pig farm. The increase trend of animal farming industry is likely to bring increasing pollution problem unless effective treatment methods are used. The main problems related to the pig farm include odor nuisance and pig manure disposal. The existing land application of piggery wastewater is the traditional way to discharge the wastewater. This may yield in land and water contamination, due to the accumulation of unused nutrients by crop plant. A case study of a large commercial pig farm from Australia is proposed to apply in smaller scale in Indonesia. Operational strategies for the small-scale SBR (Sequencing Batch Reactor) treating piggery effluent were developed based on lab-scale experiments. Due to SBR characteristics, which are money-saving and space-saving, it is very suitable to be applied in urban area. An economic evaluation was made of various process options. The cost estimation showed that SBR is a cost effective process, allowing operational batches to be adjusted to reduce unnecessary aeration cost. A reduction in the aeration cost was achieved by shortening the batch time from 24-h to 8-h. A comparison of three different SBR options showed that smaller size reactors could be more flexible and cost effective when compared with the larger ones.

HPV-associated cervical diseases: screening, methods of examination, principles of treatment

GYNECOLOGY ◽  
2019 ◽  
Vol 21 (3) ◽  
pp. 6-8
Author(s):  
Vera N Prilepskaya
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cancer Prevention ◽  
Mortality Rates ◽  
Diagnostic Methods ◽  
Screening Methods ◽  
The Past ◽  
Associated Diseases ◽  
Principles Of Treatment ◽  
Cervical Diseases

This article presents information about modern principles of diagnosis and treatment of HPV-associated diseases. Behind cervical cancer morbidity and mortality rates over the past 10 years increase significantly. Examination and observation of patients with human papillomavirus persistence of highly oncogenic types is important a link in cancer prevention. The article presents diagnostic methods, treatment of cervical diseases, as well as the possibility of pharmacotherapy in HPV-associated diseases.

Modeling the Tensile Behaviour of a SiC/SiC Composite by Master Curves

2000 ◽  
Author(s):  
J. Shi
Keyword(s):  
Master Curve ◽  
Ceramic Matrix Composites ◽  
Test Material ◽  
Tensile Behaviour ◽  
Matrix Composites ◽  
Test Results ◽  
Master Curves ◽  
The Past ◽  
Test Parameters ◽  
Tensile Data

Scatter in test results is common for relatively brittle materials such as ceramic matrix composites. The scatter may come from differences in material processing conditions, specimen machining/handling and from variations in test parameters for nominally the same test material. Large scatter in test results makes material modeling difficult. In the past, master curve concepts have been proposed to reduce scatter in tensile data and to interpret fatigue/creep results. In this paper, one such concept is examined in detail by applying it to the recent tensile test results of a SiC/SiC composite. It was found that the way to construct master curves did not apply to the CMC studied and thus a new master curve was developed to better represent the tensile data. In addition, the test data were analysed statistically based on the new master curve.

A recent overview on the porphyrin-based π-extended small molecules as donors and acceptors for high-performance organic solar cells

2021 ◽  
Author(s):  
Venkatesh Piradi ◽  
Feng Yan ◽  
Xunjin Zhu ◽  
Wai-Yeung Raymond Wong
Keyword(s):  
Solar Cells ◽  
Solar Cell ◽  
Small Molecules ◽  
Organic Solar Cells ◽  
High Performance ◽  
Cost Effective ◽  
Effective Alternative ◽  
The Past ◽  
Cost Effective Alternative ◽  
Silicon Based

Organic solar cells (OSCs) have been considered as a promising cost-effective alternative to silicon-based solar cell counterparts due to their lightweight, mechanical flexibility, and easy fabrication features. Over the past...

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