scholarly journals The effect of feeding live yeast cultures on ruminal pH and redox potential in dry cows as continuously measured by a new wireless device

2011 ◽  
Vol 56 (No. 1) ◽  
pp. 37-45 ◽  
Author(s):  
L. Křížová ◽  
M. Richter ◽  
J. Třináctý ◽  
J. Říha ◽  
D. Kumprechtová
Keyword(s):  
Redox Potential ◽  
Volatile Fatty Acids ◽  
Basal Diet ◽  
Experimental Period ◽  
Blood Parameters ◽  
Mean Value ◽  
Acid Base Balance ◽  
Ruminal Ph ◽  
Yeast Cultures ◽  
Live Yeast

An experiment was carried out on four dry Holstein cows fitted with rumen cannulas that were divided into two groups. The crossover design experiment was divided into 4 periods of 3 weeks. Each period consisted of a 17-day preliminary period followed by a 4-day experimental period. Cows were fed twice daily the total mixed ration based on maize silage and concentrate. Control cows (Control) received the basal diets while experimental animals (Yeast) received the basal diet supplemented with 3.0 g of live yeast (BIOSAF Sc 47, Lesaffre, France) at each feeding. During each experimental period ruminal pH and redox potential (Eh) were monitored continuously using a developed wireless probe. Further, in each experimental period five samples of ruminal fluid were taken at 6:30, 8:30, 10:30, 13:30 and 16:30 h to determine the content of volatile fatty acids, lactic acids and ammonia. On the last day of each period, blood samples were taken for determination of blood parameters and acid-base balance. Average daily dry matter intake throughout the experiment was 8.2 kg/day and was not affected by the treatment. The average ruminal pH in Control was 6.16 that was significantly lower than in Yeast, being 6.26 (P &lt; 0.001). The diurnal pattern of ruminal pH showed a similar trend in both groups. Mean Eh in Control (&ndash;210 mV) differed significantly from Yeast (&ndash;223 mV, P &lt; 0.001). The mean value of rH (Clark's Exponent) calculated for Control (5.33) was higher than that calculated for Yeast (5.09, P &lt; 0.001). Total VFA concentrations were on average 40.8mM in Control and 57.2mM in Yeast (P &gt; 0.05). Lactate and ammonia concentrations at individual sampling times and overall mean did not differ significantly between treatments (P &gt; 0.05). Blood pH and CO<sub>2</sub> were not affected by the treatment.

scholarly journals 79 Effect of source and dietary concentration of trace minerals on serum mineral status, ruminal pH, and ruminal volatile fatty acids in lactating Holstein dairy cows

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 71-71
Author(s):  
Nicole T Briggs ◽  
Bayissa Hatew ◽  
Michael A Steele
Keyword(s):  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Experimental Period ◽  
Rumen Fermentation ◽  
Trace Minerals ◽  
Trace Mineral ◽  
Mineral Status ◽  
Ruminal Ph ◽  
Dietary Concentration

Abstract Inorganic sources of trace minerals are commonly supplemented in dairy cow diets; however, there has been an increase in the supplementation of minerals complexed with organic compounds. These organic trace minerals are thought to have greater bioavailability which may enhance rumen fermentation and absorption. The objective of this study was to assess the effects of dietary concentration and source of supplemental trace minerals on serum trace mineral status and rumen fermentation. Six lactating Holstein cows were used in a 6 x 6 Latin square design with a 23-day adaptation and 5-day experimental period. Cows were fed the same basal diet daily except for the difference in source [organic (ORG) versus inorganic (INO)] and concentration (50%, 100%, and 200% based on NRC recommendations) of trace mineral supplemented. During the experimental period feed intake and blood were collected daily. Rumen fluid was collected on the final two days of the experimental period. Data was analyzed with PROC MIXED in SAS 9.4. Dry matter intake (18.1 ± 0.70 kg), serum mineral concentrations (Cu, Mn, Se, and Zn), and rumen pH (6.5 ± 0.64) did not differ among the treatments. However, serum concentration of Co was higher in 200% ORG compared to 50% and 100% INO and 50% ORG. Ruminal concentration of acetate was higher in 50% and 100% ORG compared to 200% ORG. Butyrate ruminal concentration was higher at 50% ORG compared to 200% ORG. Ruminal propionate concentration was higher in 50% INO and 50% ORG compared to 100% INO and 200% ORG. These findings demonstrate serum trace mineral status and ruminal pH are not tightly controlled by the source of trace minerals when supplemented at 50%, 100% and 200% of the NRC recommendations, however rumen fermentation may be affected by the dietary concentration of trace minerals in the diet.

scholarly journals Effect of cattle breed and basal diet on digestibility, rumen bacterial communities, and eating and rumination activity

2020 ◽  
Vol 98 (5) ◽  
Author(s):  
Pedro H V Carvalho ◽  
Ana C J Pinto ◽  
Danilo D Millen ◽  
Tara L Felix
Keyword(s):  
Bacterial Communities ◽  
Repeated Measures ◽  
Volatile Fatty Acids ◽  
Bacterial Species ◽  
Basal Diet ◽  
Neutral Detergent Fiber ◽  
Fecal Excretion ◽  
Feeding Time ◽  
Ruminal Ph ◽  
Post Feeding

Abstract The objectives of this study were to compare ruminal total tract digestibility, bacterial communities, and eating and rumination activity between Holstein and Angus steers fed grain- or forage-based diets. Six Holstein steers (average body weight [BW] = 483 ± 23 kg) and six Angus steers (average BW = 507 ± 29 kg), previously fitted with rumen cannulae, were fed in a crossover design with a 2 × 2 factorial arrangement of four treatments: 1) Holsteins fed a grain-based diet, 2) Holsteins fed a forage-based diet, 3) Angus fed a grain-based diet, and 4) Angus fed a forage-based diet. Each period was 35 d with 26 d of diet adaptation and 9 d of sample collection. On days 1 and 2 of collection, feeding activity was recorded for 48 h. On day 3, rumen contents were sampled to measure ruminal pH at 0, 3, 6, 12, and 18 h after feeding. A portion of the strained ruminal fluid was subsampled at 0, 3, and 6 h for volatile fatty acids (VFA) analysis. Rumen contents were subsampled at 3 h for analysis of bacterial communities. From day 4 to 8, total fecal excretion, feed, and refusals samples were collected and analyzed for dry matter (DM), neutral detergent fiber (NDF), and starch. On days 8 and 9 (0 and 3 h post-feeding, respectively), total reticulorumen evacuation was conducted and contents were weighed. Data were analyzed using the MIXED procedures in SAS (v9.4 SAS Inst. Inc., Cary, NC). Repeated measures were used to analyze changes in ruminal pH and VFA over time. There were no interactions of diet × breed (P ≥ 0.07). While the main effects of diet were expected, unique to these data is the fact that bacterial diversity and richness were reduced (P &lt; 0.01) in cattle fed grain-based diets. There was no main effect (P &gt; 0.34) of breed on total tract DM, organic matter, and starch digestibility, but Angus cattle had greater (P = 0.01) NDF digestibility than Holsteins. The increased NDF digestibility may be associated with a numerical (P = 0.08) increased numbers of bacterial species in Angus steers compared with Holstein steers. Holstein steers also spent more time (P ≤ 0.05) ruminating than Angus steers. There was no effect (P &gt; 0.80) of breed on reticulorumen content at feeding time; however, Holstein steers had greater (P = 0.04) reticulorumen content on a wet basis 3 h post-feeding. Although Holstein steers spent more time ruminating, Angus steers were better able to digest NDF when compared with Holsteins, regardless of basal diet, and this improvement may be related to changes in bacterial communities in the rumen or to rumination activity.

scholarly journals Effects of xylanase supplementation on feed intake, digestibility and ruminal fermentation in Rambouillet sheep

2016 ◽  
Vol 154 (6) ◽  
pp. 1110-1117 ◽  
Author(s):  
L. H. VALLEJO ◽  
A. Z. M. SALEM ◽  
L. M. CAMACHO ◽  
A. M. KHOLIF ◽  
M. D. MARIEZCURRENA ◽  
...  
Keyword(s):  
Feed Intake ◽  
Latin Square ◽  
Basal Diet ◽  
Experimental Period ◽  
Blood Parameters ◽  
Ruminal Fermentation ◽  
Maize Stover ◽  
Post Feeding ◽  
Feed Digestibility ◽  
Xylanase Enzyme

SUMMARYThe present study aimed to investigate the effects of adding xylanase enzyme (XY) to a basal diet containing 300 g maize stover and 700 g concentrate/kg dry matter (DM) on feed intake, ruminal fermentation, total tract and ruminal digestibility, as well as some blood parameters. Four male Rambouillet sheep (39 ± 1·8 kg body weight), with permanent rumen and duodenum cannulae were used in a 4 × 4 Latin square design. Sheep were fed a basal diet without xylanase addition (control, XY0), or with the addition of xylanase at 1 (XY1), 3 (XY3) or 6 (XY6) μl/g of diet DM for 84 days, with four 21-day experimental periods. Feed intake, digestibility and rumen fermentation parameters were determined on days 16–21 in each experimental period, and the apparent ruminal neutral detergent fibre (NDF) digestibility was determined on days 16 and 17. Treatments XY1 and XY3 increased feed intake, whereas digestibility was increased with XY6. Ruminal NDF digestibility increased when sheep were fed diets treated with xylanase. Ruminal pH, ammonia-N and acetic acid increased with xylanase treated diets. Propionic acid concentration increased with diet XY1 at 3 h post-feeding, but after 9 h post-feeding its concentration decreased in the rumen of sheep fed xylanase treated diets. Xylanase had no effect on blood urea, phosphorus and triglycerides. Addition of xylanase at 6 µl/g DM in a diet containing 300 g maize stover and 700 g concentrate/kg DM and fed to Rambouillet sheep improved feed digestibility and ruminal fermentation without affecting blood parameters.

scholarly journals Performance of Lactating Dairy Cows Fed Dried Sardines

1999 ◽  
Vol 4 (1) ◽  
pp. 35 ◽  
Author(s):  
A.S. AI-Abri ◽  
C.D. Lu ◽  
R.J. Early ◽  
A. Srikandakumar ◽  
O. Mahgoub ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Crude Protein ◽  
Volatile Fatty Acids ◽  
Control Diet ◽  
Experimental Period ◽  
Milk Composition ◽  
Ruminal Ph ◽  
Lactating Dairy Cows ◽  
Continuous Feeding

To utilize locally available feed resources with livestock production in hot climates, dried sardines were incorporated into diets for lactating dairy cows. Fourteen Holstein and 13 Australian Milk Zebu multiparous cows were used in a 70-day continuous feeding experiment. lsonitrogenous and isoenergetic diets containing either soybean meal or dried sardines (supplied 40% of dietary crude protein) were fed ad - libitum. Comparisons between diets were made during the 7-week experimental period. The experiment was conducted as a 2 x 2 factorial arrangement of treatment, with diet and breed as main factors. Ruminal pH, ammonia N, total and individual volatile fatty acids concentrations were not altered by the feeding of dried sardines. Intakes of dry matter, energy, crude protein, and acid detergent fiber were lower (P<0.05) in both breeds of cows fed dried sardines. Intakes of ether extract and ash were higher (P<0.05) in cows fed dried sardines. Although it was not statistically significant, milk production was higher (P>0.05) in Holstein cows fed dried sardines than those fed the control diet (16.2 vs 15.1 kg/d). Feeding of dried sardines did not affect milk composition and compositional yields. Milk production was higher (P<0.01) in Holstein than Australian Milk Zebu cows. Effect of diet and breed interaction on milk production was significant (P<0.01 ). Potential of feeding marine proteins may be higher for higher milk producers (Holstein) than lower producers (Australian Milk Zebu). Reproduction parameters, body condition scores, and heat-stress associated parameters were not affected by the feeding of dried sardines. This study suggests that dried sardines could be incorporated into diets of lactating dairy cows without affecting milk production.

scholarly journals Effect of aflatoxin B1 on digestibility and blood parameters in horses

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Auro Braga ◽  
Fernando Queiroz de Almeida ◽  
Vinicius Pimentel Silva ◽  
Kelly Moura Keller ◽  
Marina Prado Maciel ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Basal Diet ◽  
Experimental Period ◽  
Blood Parameters ◽  
Adaptation Period ◽  
Blood Cell Count ◽  
Collection Method ◽  
Blood Samples ◽  
Randomized Design ◽  
Completely Randomized Design

The aim of this research was to evaluate the effect of mycotoxins on the digestion of nutrients and on the hematological profile of horses. Twelve horses were used in a completely randomized design, with three treatments and four replicates. Aliquots of 50 or 100 µg kg-1 of aflatoxin B1 (AFB1) were added to a basal diet naturally containing mycotoxins. The basal diet did not contain AFB1. The 40-day evaluation included a 12-day adaptation period and a 28-day experimental period. A digestion test was carried out at the end of the experimental period, using the partial stool collection method, using LIPE® as an indicator. Blood samples were collected once a week during 4 weeks of the essay for hematological and biochemical evaluations. The amounts of mycotoxins added did not influence the intake and digestibility of the nutrients (P>0.05). The aflatoxins in the diet influenced the white blood cell count, especially mature neutrophils and the creatine kinase and alkaline phosphatase (P <0.05), which had higher activity in horses fed diets with greater toxicity.  

286 Effects of Supplementation of Quebracho Extract Supplementation on Ruminal Ph of Growing Beef Steers

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 158-159
Author(s):  
Luiz Fernando Dias Batista ◽  
Aaron B Norris ◽  
Jordan Adams ◽  
Luis O Tedeschi
Keyword(s):  
Redox Potential ◽  
Repeated Measures ◽  
Random Effect ◽  
Basal Diet ◽  
Random Coefficients ◽  
Beef Steers ◽  
Organic Acid Production ◽  
Ruminal Ph ◽  
Ad Libitum Intake ◽  
Dorsal Sac

Abstract Rumen acidosis is a common metabolic disorder occurring when organic acid production exceeds clearance capacity, reducing ruminal pH. Acidosis occurrence has been directly correlated to the ratio of concentrate to forage in the diet. However, the rates of substrate fermentation and acid absorption vary at different locations in the rumen. The objective of this study was to determine the pH in different locations of the rumen using 16 rumenally- cannulated steers (309 ± 43 kg) receiving quebracho extract (QT; Schinopsis balansae) within a grower-type diet [25:75 forage-to-concentrate, dry matter (DM) % basis]. Animals were randomly assigned to one of four dietary treatments (n = 4): QT at 0, 1, 2, and 3% of DM (QT0, QT1, QT2, and QT3). Animals were adapted to the basal diet (QT0) for 12-d before being introduced to predetermined treatments for four weeks, with feed provided twice daily to allow ad libitum intake. Weekly measurements of ruminal fluid pH and redox potential (Eh) were taken four h post-feeding using a portable pH and redox meter probe in four locations of the rumen (cranial sac, ventral sac, dorsal sac, and reticulum). Data were analyzed using a random coefficients model with the pen as a random effect and week as repeated measures. The DM intake was included as a covariate. There was no interaction among diet, location, and week (P ≥ 0.925) on pH. Overall, ruminal pH was lower for QT0 and QT1 compared to QT3 (P &lt; 0.001). Ruminal pH in the cranial sac and reticulum was greater than in the dorsal sac (5.98, 6.03, and 5.87, respectively; P = 0.001). Redox potential was lower for QT0 in week 1 than all other treatments (P = 0.042). This study indicated that pH differs among locations of the rumen regardless of QT supplementation level and days on feed.

Technical note: The Comparison of pH and Redox Potential in Different Locations in the Reticulo-Rumen of Growing Beef Steers Supplemented with Different Levels of Quebracho Extract

2021 ◽  
Author(s):  
Luiz F Batista Dias ◽  
Aaron B Norris ◽  
Jordan M Adams ◽  
Thomas B Hairgrove ◽  
Luis O Tedeschi
Keyword(s):  
Redox Potential ◽  
Repeated Measures ◽  
Random Effect ◽  
Basal Diet ◽  
Technical Note ◽  
Random Coefficients ◽  
Organic Acid Production ◽  
Ruminal Ph ◽  
Ad Libitum Intake ◽  
Dorsal Sac

Abstract Rumen acidosis is a common metabolic disorder occurring when organic acid production exceeds clearance capacity, reducing ruminal pH. The occurrence of acidosis has been directly correlated to the ratio of concentrate to forage in the diet. However, rates of substrate fermentation and acid absorption vary at different locations in the reticulo-rumen. The objective of this study was to determine the pH and redox potential (Eh) in different locations of the reticulo-rumen using 16 ruminally cannulated steers (309 ± 43 kg) receiving different supplementation levels of quebracho extract (QT; Schinopsis balansae) within a grower-type diet (CP : 13.4 %; TDN: 70.4 %, and ME : 2.55 Mcal/kg, basis dry matter [DM] basis). Animals were randomly assigned to one of four dietary treatments: QT at 0, 1, 2, and 3% of DM (QT0, QT1, QT2, and QT3; respectively), containing about 0, 0.7, 1.4, and 2.1% of condensed tannins (CT), DM basis, respectively. Animals were adapted to the basal diet for 12-d before being introduced to predetermined treatments for 4 weeks (wk), with diets provided twice daily to allow ad libitum intake. Weekly measurements of ruminal fluid pH and Eh were taken 4 h post-feeding using a portable pH meter with 2 probes (pH and redox) in four locations of the reticulo-rumen (reticulum, cranial sac, dorsal sac, and ventral sac). Data were analyzed using a random coefficients model with the pen as a random effect and wk as repeated measures, with DM intake included as a covariate. There was no interaction among treatments, location, and wk (P ≥ 0.882) on reticulo-ruminal pH. Overall, ruminal pH was lower for QT0 and QT1 compared to QT3 (P &lt; 0.001). The pH in the reticulum was greater than those of the ventral and dorsal sacs (6.05 vs. 5.94, 5.89, respectively; P ≤ 0.001) but similar to cranial sac (6.00). Reticular pH was positively correlated with the ruminal locations ( ≥ 0.78; P &lt; 0.001). The linear equation to estimate ruminal mean pH using reticulum pH had an intercept and slope different from zero (P ≤ 0.04), but CT (% DM) was not different from zero (P = 0.15), root mean square error of 0.15, and R 2 of 0.778: 0.723 (±0.36) + 0.857 (±0.059) × Reticulum pH + 0.033 (±0.023) × CT. The redox potential was lower for QT0 in wk 1 than all other treatments (P &lt; 0.001). We concluded that reticulo-ruminal pH differs among locations in the rumen regardless of QT supplementation level and days on feed, with reticular pH being the highest.

scholarly journals Using different levels of energy and protein and their effects on bodyweight and blood chemistry of ostriches

2021 ◽  
Author(s):  
Tahereh Nikravesh-Masouleh ◽  
Alireza Seidavi ◽  
Magdalena Solka ◽  
Mohammad Dadashbeiki
Keyword(s):  
Weight Gain ◽  
Total Cholesterol ◽  
Feed Intake ◽  
Experimental Period ◽  
Blood Chemistry ◽  
Blood Parameters ◽  
Bodyweight Gain ◽  
Transferase Activity ◽  
Dietary Energy ◽  
Protein Levels

AbstractTo determine the effect of different dietary energy and protein levels on bodyweight and blood chemistry, 36 ostriches at 2 to 9 weeks of age for feeding conditions and 18 for blood chemistry parameters was used. The birds were divided into six treatment groups. Energy and protein levels of diet were 2400 and 2600 kcal/kg and 20%, 22%, and 24%, respectively. The feed intake and bodyweight gain were determined a weekly. Blood chemical parameters including glucose, HDL, LDL, total cholesterol, triglycerides, total protein, albumin, globulin, aspartate amino-transferase and alanine amino-transferase activity were determined. The highest weight gain during the whole experiment was observed in ostriches offered 2400 kcal · kg−1 dietary energy and 20% protein. The lowest level of total cholesterol and protein was observed in treatment V (2600 kcal · kg−1 dietary energy and 22% protein). The lowest level of glucose and triglycerides was noted after treatment I. The highest albumin and globulin concentrations were in treatment III (2400 kcal · kg−1 dietary energy and 24% protein) and treatment II (2400 kcal · kg−1 dietary energy and 22% protein), respectively. The energy level had no effect (P < 0.05) on feed intake and weight gain in all experimental period. The results of this study showed that with increasing energy and protein levels, most blood parameters increased in ostriches but total cholesterol did not.

scholarly journals Live Yeast or Live Yeast Combined with Zinc Oxide Enhanced Growth Performance, Antioxidative Capacity, Immunoglobulins and Gut Health in Nursery Pigs

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1626
Author(s):  
Shenfei Long ◽  
Tengfei He ◽  
Sung Woo Kim ◽  
Qinghui Shang ◽  
Tadele Kiros ◽  
...  
Keyword(s):  
Average Daily Gain ◽  
Basal Diet ◽  
Conversion Ratio ◽  
Gut Health ◽  
Total Volatile ◽  
Feed Conversion ◽  
Nursery Pigs ◽  
Serum Iga ◽  
Daily Gain ◽  
Live Yeast

This study aimed to investigate the effects of dietary LY or LY combined with ZnO supplementation on performance and gut health in nursery pigs. 192 Duroc × Landrace × Yorkshire piglets (weaned on d 32 of the age with 9.2 ± 1.7 kg BW) were allocated into four treatments with eight replicate pens, six piglets per pen. The treatments included a basal diet as control (CTR), an antibiotic plus ZnO diet (CTC-ZnO, basal diet + 75 mg/kg of chlortetracycline + ZnO (2000 mg/kg from d 1 to 14, 160 mg/kg from d 15 to 28)), a LY diet (LY, basal diet + 2 g/kg LY), and a LY plus ZnO diet (LY-ZnO, basal diet + 1 g/kg LY + ZnO). The results showed that pigs fed LY or LY-ZnO had increased (p < 0.05) average daily gain, serum IgA, IgG, superoxide dismutase, fecal butyric acid, and total volatile fatty acid concentrations, as well as decreased (p < 0.05) feed conversion ratio and diarrhea rate compared with CTR. In conclusion, pigs fed diets with LY or LY combined with ZnO had similar improvement to the use of antibiotics and ZnO in performance, antioxidant status, immunoglobulins, and gut health in nursery pigs.

PSVII-23 Experimental evaluation of the effectiveness of the Quercus cortex extract in the carp feeding system

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 339-339
Author(s):  
Mariya Miroshnikova ◽  
Elena Miroshnikova ◽  
Alexey Sizentsov ◽  
Azamat Arinzhanov ◽  
Yuliya Kilyakova
Keyword(s):  
Calcium Content ◽  
Fish Farming ◽  
Basal Diet ◽  
Experimental Period ◽  
Atomic Emission ◽  
Essential Elements ◽  
Control Group ◽  
Metagenomic Sequencing ◽  
Microbial Profile ◽  
Combined Feed

Abstract One of the most promising ways to improve the effectiveness of fish farming is the use of phytobiotics in the diet of fish. On this basis, we set the aim to evaluate the effectiveness of the Quercus cortex extract in the concentration of 1 mg/kg on biological features and the productivity of carp. The object of the research was yearling carp grown in the conditions of Orenburgskiy Osetr LLC. Two groups (n = 20) were formed by the method of analogs to conduct the research. After the adaption period (7 days), the groups came to the experimental period (35 days). The feed KRK-110–1 produced by PJSC Orenburg Combined-Feed Plant was used as the basal diet. The live fish were monitored weekly by individual weighing during the accounting period. The elemental composition was determined by the method of atomic emission and mass spectrometry (ICP-AES and ICP-MS). The microflora analysis was carried out by the method of metagenomic sequencing. The use of the Quercus cortex extract in the diet in the studied concentration increased body weight by 15.1% (P ≤ 0.05) compared to the control group. There was experimentally revealed a stimulating effect on the population growth of individual representatives of the microbiome (Luteolibacter, Lactococcus) (P ≤ 0.05) without significantly changing the overall picture of the microbial profile, which, in our view, affects the metabolic processes, in particular, by stimulating the formation of biologically available forms of essential elements and their subsequent accumulation in the tissues of the studied fish. Thus, the experimental group found: (against the background of an increase in the total mineralization (ash residue) by 17.95 % (P ≤ 0.05)) the calcium content increased by 133.9% (P ≤ 0.05), phosphorus by 83% (P ≤ 0.05), iron by 337.7% (P ≤ 0.05), respectively, in comparison with the control group.

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