scholarly journals Identification on Histamine Content and Histamin-Forming Bacteria of Boiled Badeng Slender Tuna

Author(s):  
Yuliana Syalviana Fatuni ◽  
Ruddy - Suwandi ◽  
Agoes Mardiono Jacoeb

Pindang is a semi-dried product which relatively short lasting products. Due to the rapid formation of histamine and bacterial enzyme activities contained in the fish, it is necessary to study and identify the histamine levels either on fresh tuna or in its pindang. This study aims to analyze histamine and histamine-forming bacteria in processed pindang tuna (A. rochei). Fresh tuna (A. rochei) was obtained from TPI Cisolok Palabuhanratu. The fish was processed according to pindang badeng method (20% salt added, boiled for 8 hours). The product then stored at room temperature and observed for 0,8,16,24 and 32 hours. Analysis were conducted through chemical (histamine and TVB) and microbiological test (TPC, identification of bacteria, histamine levels in bacteria). The results showed that the stored pindang for 32 hours can reactivate the production of histamine and increase their levels significantly. This is consistent with the increase on the value of TVB and TPC in pindang products. Six types of histamine-forming bacteria found on pindang tuna that are P. vulgaris, H. alvei, M. Morganii, E. aerogenes, K. oxytoca, K. pneumoniae. Activity test results showed that K. pneumoniae and H. alvei form the highest and the lowest levels of histamine formation respectively.<br />Keywords: Histamine, bacterial, boiled tuna (Auxis rochei)

Author(s):  
Naomi Katayama ◽  
Akemi Ito ◽  
Mayumi Hirabayashi ◽  
Natuki Sasaki ◽  
Moe Inuzuka

Sanitary control of cutting boards in the kitchen is important to prevent food poisoning. To preventing secondary and tertiary contamination of food poisoning bacteria, it is necessary to know the hygiene status of cooking utensils. Therefore, in this study, we compared the values after cooking, washing, and spraying 70% alcohol on cutting boards for vegetables using the ATP test and microbiological test. As a result, the ATP value after spraying with alcohol was 100 or less, which was better than that after washing. Microbial test results showed that microorganisms were present on the vegetable cutting board even after spraying with 70% alcohol. Since microorganisms are present even after spraying with alcohol, it is possible that the growth of microorganisms will occur again if the vegetable cutting board left in a moist state at room temperature. When using a vegetable cutting board left unattended, it is necessary to wash repeatedly and spray it with alcohol.


Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 330
Author(s):  
Pan Ma ◽  
Pengcheng Ji ◽  
Yandong Jia ◽  
Xuerong Shi ◽  
Zhishui Yu ◽  
...  

The Al-20Si-5Fe-3Cu-1Mg alloy was fabricated using selective laser melting (SLM). The microstructure and properties of the as-prepared SLM, post-treated SLM, and SLM with substrate plate heating are studied. The as-prepared SLM sample shows a non-uniform microstructure with four different phases: fcc-αAl, eutectic Al-Si, Al2MgSi, and δ-Al4FeSi2. With thermal treatment, the phases become coarser and the δ-Al4FeSi2 phase transforms partially to β-Al5FeSi. The sample produced with SLM substrate plate heating shows a relatively uniform microstructure without a distinct difference between hatch overlaps and track cores. Room temperature compression test results show that an as-prepared SLM sample reaches a maximum strength (862 MPa) compared to the heat-treated (524 MPa) and substrate plate heated samples (474 MPa) due to the presence of fine microstructure and the internal stresses. The reduction in strength of the sample produced with substrate plate heating is due to the coarsening of the microstructure, but the plastic deformation shows an improvement (20%). The present observations suggest that substrate plate heating can be effectively employed not only to minimize the internal stresses (by impacting the cooling rate of the process) but can also be used to modulate the mechanical properties in a controlled fashion.


2011 ◽  
Vol 391-392 ◽  
pp. 1445-1449
Author(s):  
Chun Hua Zhang ◽  
Shi Lin Luan ◽  
Xiu Song Qian ◽  
Bao Hua Sun ◽  
Wen Sheng Zhang

The influences of low temperature on the interlaminar properties for PBO fiber/epoxy composites have been studied at liquid nitrogen temperature (77 K) in terms of three point bending test. Results showed that the interlaminar shear strength at 77 K were significantly higher than those at room temperature (RT). For the analysis of the test results, the tensile behaviors of epoxy resin at both room temperature and liquid nitrogen temperature were investigated. The interface between fiber and matrix was observed using SEM images.


2021 ◽  
Vol 324 ◽  
pp. 01011
Author(s):  
Eko Prayetno ◽  
Tonny Suhendra ◽  
Jeremya Lukmanto Saputra

Fish is one of the high-protein foods that are very helpful for the development of the human brain. Then, it is necessary to maintain the freshness of the fish for consumption. At this time, fishers and fishmongers preserve the freshness of fish by using Ice in the fish storage. However, it is considered ineffective due to improper ice change time. Therefore, monitoring temperature is very important and helpful to find the right time when replacing the Ice used to ensure the quality of fish. The development of this device uses Arduino ESP32, DHT21 Sensor, Micro SD Module, Internet of Things system, monitoring using Blynk Application and notifications using Telegram App. DHT21 sensor test results obtained a data conformity level (Error Level) of 2%. At the fish storage room temperature, there is the lowest temperature of 10.50 oC and ice temperature conditions in the storage of 0 oC. Therefore, the best state to keep fish fresh that researchers want is 0 oC to 2 oC at ice temperatures or 11.50 oC obtained in testing the time it takes to replace Ice by about 10 hours.


2020 ◽  
Vol 25 (1) ◽  
pp. 52
Author(s):  
Alston Millan

Nutmeg oleoresin microcapsule is the solid, liquid, and gas coating technology of the nutmeg fruit.  The purpose of this study was to know how mace nutmeg oleoresin microcapsules could preserve the broiler chicken meat at room temperature during 4 days of observation. The experimental design used was a Completely Randomized Design (CRD) consisting of 5 treatments with mace concentration of nutmeg microcapsules oleoresin (0, 50, 100, 150, and 200) ppm. The variables observed were water content, pH value, total microbes, and organoleptic properties (color, aroma, and texture). The results showed that mace nutmeg oleoresin microcapsules did not affect (p> 0.05)  water content and sensory properties of broiler chicken meat, but had a significant effect (p <0.05) on the pH value and total microbe at the same day of room temperature storage. Panel organoleptic test results on the level of preference for meat color, aroma, and texture of chicken meat were in the range score of 3 (somewhat like) to 4 (somewhat dislike). The treatments of nutmeg oleoresin did not affect (p> 0.05) on color, the aroma, and the texture of broiler chicken meat. This research shows that marinating using oleoresin microcapsules has not been effective as a preservative to broiler chicken meat at room temperature. Keywords: chicken meat, mace nutmeg oleoresin microcapsules


Author(s):  
Kang Zhao ◽  
Jucun Huang ◽  
Dan Dai ◽  
Yuwei Feng ◽  
Liming Liu ◽  
...  

AbstractWe report a case of acute myelitis in a patient infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A 66-year-old man with coronavirus disease 2019 was admitted with acute flaccid paralysis of the bilateral lower limbs and urinary and bowel incontinence. All serum microbiological test results were negative, except for SARS-CoV-2 nucleic acid testing. Clinical findings indicated post-infectious acute myelitis. He received treatment containing ganciclovir, lopinavir/ritonavir, moxifloxacin, dexamethasone, human immunoglobulin, and mecobalamin. With a diagnosis of post-infectious acute myelitis and comprehensive treatment, paralysis of the bilateral lower extremities ameliorated. After two negative novel coronavirus RNA nasopharyngeal swab tests, he was discharged and transferred to a designated hospital for isolation and rehabilitation therapy.


2018 ◽  
Vol 12 (1) ◽  
pp. 1-8
Author(s):  
J. Bright Brabin Winsley ◽  
M. Muthukannan

Background and Objective: The demand for course aggregate is increasing every day. Natural aggregate used for ordinary concrete is obtained by quarrying, which cause serious environmental issues. An alternate course aggregate is needed for sustainable development. The objective of this research is to produce an alternative course aggregate in combination with soil available locally near site along with fly ash, to test its properties to make it fit for concrete. Method: An alternative coarse aggregate is produced from red soil and fly ash mixed at various ratios, 100:0, 90:10, 80:20, 70:30, 60:40, 50:50, fresh aggregate granules of different sizes less than 10mm is prepared using hand press, the aggregates were sundried in shade for 24hours, oven dried at 110°C, burned in Muffle furnace at temperature of 950°C and cooled gradually to reach room temperature. After the production, the specific gravity, bulk density, water absorption, Impact and aggregate crushing of the aggregates were tested. Result: Test results showed that aggregates produced are of lesser specific gravity, density with relatively appreciable impact value and crushing value. Conclusion: The test results show that the aggregates produced can be used in construction as replacement for natural aggregates.


2019 ◽  
Vol 26 (1) ◽  
pp. 388-393
Author(s):  
M.J. Shen ◽  
M.F. Zhang ◽  
T. Ying

AbstractThe present work aims to research the treatment processing of magnesium reinforced with 1 μmsilicon carbide particle (SiCp) using stir casting combined by ultrasonic vibration. Present studies have been done on six different materials: (a) AZ31B alloy without particles, (b) 5 vol.% SiCp/AZ31B composites fabricated with different semi-solid stirring time (5 min, 10 min, 15 min and 20 min), (c) composite with 20 vol.% SiCp. The effects of 1 μm/SiCp pretreatment and stirring time on microstructure and interfacial wettability as well as mechanical properties of the materials were confirmed. Both short and long stirring time for the particle dispersion brought particle agglomeration. Results of SEM microstructure and tensile properties exhibited that the optimal stirring parameters of 625 °C/1500 rpm/15 min are exploited, and 20 vol.% SiCp/AZ31B composite was fabricated by the optimal stirring parameters. The application of optimal stirring parameters for the treatment resulted in homogeneous particle distribution. The addition of SiCp leads to a reduced matrix grain, and 20 vol.% SiCp/AZ31B composite showed smaller grain size than. 5 vol.% SiCp/AZ31B composite. The interface between SiCp and matrix is clear and interfacial wettability well. Tensile test results show that with increasing SiCp content, strengths increase while ductility decreases.


2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Yanhui Zhong ◽  
Yilong Wang ◽  
Bei Zhang ◽  
Xiaolong Li ◽  
Songtao Li ◽  
...  

The rapid detection of asphalt content in asphalt mixture is of great significance to the quality evaluation of asphalt pavement. Based on the dielectric properties of an asphalt mixture, the prediction model of asphalt content is deduced theoretically using three types of dielectric models: Lichtenecker-Rother (L-R) model, Rayleigh model, and Bottcher equation. Under the condition of laboratory mixing at room temperature (about 20–25°C), a dielectric test of asphalt mixture is conducted to verify the applicability of the model. The test results indicate that the dielectric constant of the asphalt mixture is inversely proportional to the asphalt content and directly proportional to the aggregate size of the mixture. Among the models, the Rayleigh model has a wide range of applications and exhibits a high accuracy, with an average relative error of only 1.86%. The results provide a theoretical basis for the nondestructive testing of asphalt pavements using ground-penetrating radar.


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