scholarly journals Histomorphological effects of sodium arsenite on uterus of rats

2020 ◽  
Vol 9 (11) ◽  
pp. 1641
Author(s):  
Amaidah Mir ◽  
Hammad Ahmed Butt ◽  
Maria Yasmeen ◽  
Anber Saleem ◽  
Ruqqia Shafi Minhas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Sodium Arsenite ◽  
Developed Countries ◽  
Control Group ◽  
Toxic Agent ◽  
Luminal Diameter ◽  
Epithelial Component ◽  
Group B ◽  
Uterine Glands ◽  
Experimental Group

Background: Arsenic is highly toxic agent and a risk factor for disease and disability. Arsenic is present in drinking water of many developing and developed countries including Pakistan and due to rapid industrialization its quantity in soil and water is increasing day by day.Methods: In an 18 month study in which we took two principal groups, labelled as control group A and experimental group B. The animals of experimental group B were administered 4 µg of sodium arsenite dissolved in 10 ml of distilled water by oral gavage daily for 14 days. The uterus was removed and processed for paraffin embedding and stained with hematoxylin and eosin (H and E). The histological parameters; uterine luminal diameter, height of uterine luminal epithelium, area occupied by epithelial component of uterine glands and the thickness of myometrium were measured and evaluated by civil AutoCAD 2013 software. The data was analyzed statistically with the statistical package for social sciences (SPSS).Results: Histological results showed the degenerative effects. The luminal diameter of uterine horns was reduced in experimental animals. The height of uterine epithelium was reduced. Area occupied by epithelial component of uterine glands was reduced along the reduction in the thickness of myometrium.Conclusions: The histological abnormalities observed in uterus showed that the degenerative effects may be due to oxidative stress produced by the exposure to sodium arsenite. As sodium arsenite produces the oxidative stress by the formation of free radicals and by the denaturation of proteins.

scholarly journals Nephroprotective Effects of L-Arginine against Chemotherapy Induced Acute Kidney Injury in Wistar Rats

2020 ◽  
Vol 9 (4) ◽  
pp. 249-255
Author(s):  
Dr.Kumayl Abbas Meghji ◽  
Dr.Tariq Feroz Memon ◽  
Dr. Imtiaz Ahmed ◽  
Dr. Sehar Gul Memon ◽  
Dr. Naila Noor ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Acute Kidney Injury ◽  
Wistar Rats ◽  
Kidney Injury ◽  
Control Group ◽  
P Value ◽  
Renal Histology ◽  
Renal Markers ◽  
Group B ◽  
Experimental Group

Objective To evaluate the protective role of L-Arginine in cisplatin induced acute renal injury through assessment of renal, oxidative stress and inflammatory markers in albino wistar rats. Methods: Quasi-experimental study was conducted at the department of physiology and postgraduate research laboratory at Isra University, Hyderabad, Sindh from April 2019 to September 2019. Thirty male Albino wistar rats were selected through non-random purposive sampling and divided equally into three different groups: Group-A (Control group), Group-B (experimental group) received Cisplatin alone and Group-C (experimental group) received Cisplatin along with arginine. After sacrificing the animals, blood samples were collected through cardiac puncture while renal histopathological analysis was under the light microscope. The changes in severity were observed using a graded scale. Data was analysed using SPSS v23.0. Results: There was a statistically significant (p-value <0.05) decline in the bodyweight and rise in absolute kidney weight of group B in comparison with other two group. Moreover, significant rise (p-value <0.05) in serum renal markers was observed in group B while significant decline (p-value <0.05)  in these serum renal markers in group C compared with group B.  Furthermore, prominent demage in normal renal histology in group B rats while restoration of renal histology was demonstrated in group C rats. Conclusion: The present study concludes that L-Arginine exerts an anti-oxidative, anti-inflammatory and nephro-protective effect for renal tissue damage caused by Cisplatin. Keywords: Acute Kidney Injury, Antioxidant, Cisplatin, L-Arginine, Oxidative stress

scholarly journals LAGENARIA SICERARIA;

2019 ◽  
Vol 26 (05) ◽  
Author(s):  
Saima Qureshi ◽  
Khadija Qamar ◽  
Tassaduq Hussain
Keyword(s):  
Body Weight ◽  
Sodium Arsenite ◽  
Control Group ◽  
P Value ◽  
Lagenaria Siceraria ◽  
Sprague Dawley ◽  
Group A ◽  
Group B ◽  
Group D ◽  
Experimental Group

Objectives: To observe the effect of lagenaria siceraria on inflammation and fibrosis brought about by arsenic in liver of Sprague Dawley rat. Study Design: Laboratory based randomized control trial. Place and Duration of Study: This experiment was performed at Department of Anatomy, Army Medical College Rawalpindi in co-operation with National Institute of Health (NIH) Islamabad for eight weeks (1st March 2017 to 25th April 2017). Material and Methods: Fifty Sprague Dawley rats (both male and females housed separately) were carefully chosen and distributed randomly into five groups, each consisting of 10 animals. A and B were the control groups whereas C, D and E served as experimental groups. During the first four weeks, experiment groups C, D and E were given a dosage of 5milligram/kilogram body weight of sodium arsenite. At the end of four weeks, animals from control group A and experimental group C were dissected and liver samples were processed for microscopic studies. In the next 4 weeks, group D animals were set aside without any further intervention. At that time, sodium arsenite at a dose of 5 milligram/kilogram body weight and lagenaria siceraria at a dose of 100 milligram/kilogram bodyweight were administered to group E animals. Group B animals served as control for experimental groups D and E. At the end of these 4 weeks animals of groups B, D and E were dissected. Liver was processed, fixed and stained for microscopic study. Area of portal triad as well as liver lobules were studied for inflammation and fibrosis and results were analysed. Statistical tool used to analyse the data was SPSS v 22. Results were considered to be significant when p-value is ≤ 0.05. Results: Experimental group C developed moderate grade fibrosis and inflammation (grade 2 to 3) as compared to group A. Degree of inflammation and fibrosis was mild to moderate (grade 1 to 2) in group D. There was no inflammation and fibrosis (Grade 0 to 1) in group E. Group B served as a control for group D and E. Conclusion: Inflammation and fibrosis developed in the liver of adult rats when they were subjected to sodium arsenite even for a brief calculated period. Simultaneous administration of lagenaria siceraria can shield and diminish the toxic effects of arsenic. Oxidative potential and immunomodulatory properties and presence of flavonoid like substances renders lagenaria siceraria to act as ameliorative against this fibrosis and inflammation in liver lobules and surrounding area of portal triads.

Glycine and L-Arginine supplementation ameliorates gastro-duodenal toxicity in a rat model of NSAID (Diclofenac)-gastroenteropathy via inhibition of oxidative stress

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Akinleye Stephen Akinrinde ◽  
Halimot Olawalarami Hameed
Keyword(s):  
Oxidative Stress ◽  
Total Protein ◽  
Therapeutic Index ◽  
Control Treatment ◽  
Control Group ◽  
Protective Effects ◽  
Serum Total Protein ◽  
Significant Amelioration ◽  
Group A ◽  
Group B

Abstract Objectives This study examined the possible protective roles of exogenous glycine (Gly) and L-Arginine (l-Arg) against Diclofenac (DIC)-induced gastro-duodenal damage in rats. Methods Rats were divided into Group A (control), Group B (DIC group) and Groups C–F which were pre-treated for five days with Gly1 (250 mg/kg), Gly2 (500 mg/kg), l-Arg1 (200 mg/kg) and l-Arg2 (400 mg/kg), respectively, before co-treatment with DIC for another three days. Hematological, biochemical and histopathological analyses were then carried out. Results DIC produced significant (p<0.05) reduction in PCV (13.82%), Hb (46.58%), RBC (30.53%), serum total protein (32.72%), albumin (28.44%) and globulin (38.01%) along with significant (p<0.05) elevation of serum MPO activity (83.30%), when compared with control. In addition, DIC increased gastric H2O2 and MDA levels by 33.93 and 48.59%, respectively, while the duodenal levels of the same parameters increased by 19.43 and 85.56%, respectively. Moreover, SOD, GPx and GST activities in the DIC group were significantly (p<0.05) reduced in the stomach (21.12, 24.35 and 51.28%, respectively) and duodenum (30.59, 16.35 and 37.90%, respectively), compared to control. Treatment with Gly and l-Arg resulted in significant amelioration of the DIC-induced alterations although l-Arg produced better amelioration of RBC (29.78%), total protein (10.12%), albumin (9.93%) and MPO (65.01%), compared to the DIC group. The protective effects of both amino acids against oxidative stress parameters and histological lesions were largely similar. Conclusions The data from this study suggest that Gly or l-Arg prevented DIC-induced gastro-duodenal toxicity and might, therefore be useful in improving the therapeutic index of DIC.

EFFECT OF YOGA ON CARDIOVASCULAR EFFICIENCY

2019 ◽  
pp. 12-16
Author(s):  
Brij Bhushan Singh ◽  
Shubi Mirja ◽  
Samiya Husain
Keyword(s):  
Statistical Technique ◽  
Step Test ◽  
Control Group ◽  
Significant Difference ◽  
Post Test ◽  
Group A ◽  
Cardio Vascular ◽  
Level Of Significance ◽  
Group B ◽  
Experimental Group

The purpose of this study was to investigate the effects of yogic practices on cardio-vascular efficiency. Material and methods: Total Forty (40) subject’s boys/girls were selected as the sample of the study through the random sampling and their age ranged between 20 to 25 years from the Department of Physical Education, Aligarh Muslim University, Aligarh. The subjects were divided into two groups comprising 20 subjects in each group, namely group “A” (the experimental group) performs yogic practices (Asanas, Pranayamas and Kriyas) and group “B” (the control group) served as control. Statistical technique: for the assessment of the cardiovascular efficiency through Harvard step test pre and post-test was conducted and t-test was applied for the analysis. Result: revealed that there exist a significant difference between group A and group B, at .05 level of significance. On the basis of the pre and post-test among experimental and control group, experimental group found better than the control group on cardiovascular efficiency and significant result were found in this study.

scholarly journals Ameliorative effects of Spinacia oleracea on sperm morphology, count, and motility by normalizing the obesity-induced oxidative stress in Sprague Dawley rats

2020 ◽  
Vol 14 (03) ◽  
pp. 124-127
Author(s):  
Somia Iqbal ◽  
Noman Sadiq ◽  
Saad Siddiqui ◽  
Hira Iqbal
Keyword(s):  
Sperm Concentration ◽  
Treated Group ◽  
Sperm Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Normal Morphology ◽  
Sprague Dawley Rats ◽  
Group A ◽  
Group B ◽  
Experimental Group

Background: Obesity is a prevailing metabolic disorder that affects the functioning of the male reproductive system. Excessive adipose tissue enhances reactive oxygen species generation and is linked with male infertility. Spinach has demonstrated antioxidant effects. The present study was conducted to determine the antioxidant effects of spinach on sperm parameters in obese Sprague Dawley rats. Subjects and methods: This randomized control study was conducted at the animal house of the National Institute of Health Islamabad, Islamic International Medical College, Cosmesurge International Hospital, Rawalpindi, and Apollo lab, Islamabad, Pakistan from April 2016 to March 2017. Forty male Sprague Dawley rats having an age of 8 weeks and weight 160-200g were tagged from number 1 to 40. Every third rat was randomly allocated to control Group A (n=13) and remaining into the Experimental group (n=27). Rats of control Group A was given a standard diet while a high-fat diet was given to Experimental group rats to induce obesity for the duration of six weeks. Weight (g) was measured weekly and obesity was confirmed when rats attain more than 20% weight when compared with that of rats of control Group A. Then, after obesity induction, the experimental group was alienated into the obesity control group (Group B) and spinach treated group (Group C). For sample, rats of Group A and Group B were sacrificed, and the cauda epididymis of each rat was placed in a Petri dish containing normal saline and cut into pieces to allow the release of sperm and then sperm parameters (sperms concentration, motility, and morphology) were recorded under the microscope. Then, spinach (5% hot water extract) along with the persistence of fat diet was administered to Group C for 4 weeks and finally, sperm parameters were measured in this group. Results: Sperm concentration/ml, motility (%), and normal morphology (%) of Group B rats were significantly decreased as compared to Group A rats. However, sperm concentration/ml, motility (%), and normal morphology (%) of Group C (spinach treated group) rats was significantly increased (p<0.001) as compared to Group B (obesity control group) rats after administering spinach. Conclusion: The addition of Spinach in a normal diet regimen restores normal sperm morphology, improves sperm motility and concentration.

scholarly journals A Subpressor Dose of Angiotensin II Elevates Blood Pressure in a Normotensive Rat Model by Oxidative Stress

2015 ◽  
pp. 153-159 ◽  
Author(s):  
M. M. GOVENDER ◽  
A. NADAR
Keyword(s):  
Oxidative Stress ◽  
Blood Pressure ◽  
Angiotensin Ii ◽  
Mrna Expression ◽  
Rat Model ◽  
Wistar Rat ◽  
Control Group ◽  
Sod Activity ◽  
Male Wistar Rats ◽  
Experimental Group

Oxidative stress is an imbalance between free radicals and antioxidants, and is an important etiological factor in the development of hypertension. Recent experimental evidence suggests that subpressor doses of angiotensin II elevate oxidative stress and blood pressure. We aimed to investigate the oxidative stress related mechanism by which a subpressor dose of angiotensin II induces hypertension in a normotensive rat model. Normotensive male Wistar rats were infused with a subpressor dose of angiotensin II for 28 days. The control group was sham operated and infused with saline only. Plasma angiotensin II and H2O2 levels, whole-blood glutathione peroxidase, and AT-1a, Cu/Zn SOD, and p22phox mRNA expression in the aorta was assessed. Systolic and diastolic blood pressures were elevated in the experimental group. There was no change in angiotensin II levels, but a significant increase in AT-1a mRNA expression was found in the experimental group. mRNA expression of p22phox was increased significantly and Cu/Zn SOD decreased significantly in the experimental group. There was no significant change to the H2O2 and GPx levels. Angiotensin II manipulates the free radical-antioxidant balance in the vasculature by selectively increasing O2− production and decreasing SOD activity and causes an oxidative stress induced elevation in blood pressure in the Wistar rat.

scholarly journals PENGARUH PENDEKATAN FONIK TERHADAP KEMAMPUAN MEMBACA AWAL PADA ANAK KELOMPOK B DI TK GUGUS III KECAMATAN BULELENG TAHUN PELAJARAN 2017/2018

2018 ◽  
Vol 6 (2) ◽  
Author(s):  
Rina Ayu Widiasari . ◽  
Prof. Dr. Ni Ketut Suarni, M.S., Kons. . ◽  
Dr. Putu Aditya Antara, S.Pd., M.Pd. .
Keyword(s):  
Reading Ability ◽  
Control Design ◽  
Early Reading ◽  
Average Score ◽  
Control Group ◽  
Significance Level ◽  
Post Test ◽  
Group B ◽  
Academic Year ◽  
Experimental Group

Penelitian ini bertujuan untuk mengetahui pengaruh fonik terhadap kemampuan membaca awal pada kelompok B TK Gugus III Kecamatan Buleleng tahun ajaran 2017/2018. Penelitian ini merupakan quasi eksperimen dengan desain non-equaivalent post-test only control design. Metode yang digunakan dalam pengumpulan data adalah lembar observasi. Populasi dalam penelitian ini berjumlah 227 anak dan sampelnya 26 anak. TK Dana Punia sebagai kelompok Eksperimen dan TK Tunas Harapan sebagai kelompok Kontrol. Hasil penelitian menunjukkan bahwa rerata skor hasil setelah diberikan perlakuan didapatkan post-test kelompok eksperimen adalah 62,23 dan kelompok kontrol menggunakan random adalah 42,00, data dianalisis menggunakan uji-t, maka diperoleh thitung yaitu (7,60) dengan taraf signifikansi 5% diperoleh ttabel yaitu (1,17). Karena ttabel > thitung berarti H0 ditolak dan H1 diterima. Dinyatakan bahwa anak yang mendapat perlakuan pendekatan fonik ada perbedaan signifikan dengan anak yang tidak mendapat perlakuan terhadap kemampuan membaca awal anak. Disarankan bahwa pendekatan fonik dapat digunakan sebagai alternatif dalam pembelajaran. Dengan demikian dapat disimpulkan bahwa ada pengaruh pendekatan fonik terhadap kemampuan mebaca awal anak kelompok B.Kata Kunci : pendekatan fonik, membaca awal, pembelajaran aktif This study aims to determine the effect of phonic on early reading ability in group B TK Gugus III Buleleng District academic year 2017/2018. This research is a quasi experiment with non-equaivalent design post-test only control design. The method used in data collection is the observation sheet. The population in this study amounted to 227 children and the sample was 26 children. TK Dana Punia as experiment group and Tunas Harapan kindergarten as a control group. The results showed that the average score of the results after the treatment was obtained post-test experimental group is 62.23 and the control group using the random is 42.00, the data is analyzed using the t-test, then obtained tcount ie (7,60) with significance level 5 % obtained ttable ie (1.17). Because ttable> titung means H0 is rejected and H1 accepted. It was stated that children who received phonic approach were significantly different from children who were not treated for early reading ability. It is suggested that the phonic approach can be used as an alternative in learning. Thus it can be concluded that there is influence of phonic approach to ability mebaca early child group B.keyword : Keywords: phonic approach, early reading, active learning

Effects of Lead Nitrate Induced Histological Changes in Liver of Male Sprague Dawley Rats

2021 ◽  
Vol 15 (11) ◽  
pp. 2948-2950
Author(s):  
Sumaira Abbasi ◽  
Mushtaq Ahmad ◽  
Kaukab Anjum ◽  
Amaidah Mir ◽  
Ayesha Irfan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radicals ◽  
Lead Nitrate ◽  
Normal Diet ◽  
The Body ◽  
Central Vein ◽  
Sprague Dawley ◽  
Toxic Agent ◽  
Sprague Dawley Rats ◽  
Group B

Lead is a highly toxic agent and a potent risk factor for various diseases as its quantity in an environment is increasing day by day. Aim: To observe and analyze the lead nitrate induced histomorphological changes in the liver of Sprague Dawley rats. Study Design: Experimental Study. Methodology: Animals of group A (control) were fed on normal diet but the animals of group B were given 50mg/kg of lead nitrate dissolved in 10ml of distilled water through oral gavage for 14 days daily. SPSS version 22 was used for data analysis. All the quantitative data was expressed as means ± SD. One Way ANOVA followed by Post Hoc Tukey test was applied. Results: Degenerative effects were noted. The number of Inflammatory and Kupfffer cells is increased with decreased in the body weight. Steatosis and central vein congestion were also present. Conclusion: It was concluded that degenerative effects histologically may be due to oxidative stress produced by formation of free radicals and denaturation of proteins by lead nitrate. Keywords: Lead, Liver, Central Vein Congestion, Oxidative Stress and Free Radicals.

The Effectiveness of Scaffolding in a Web-Based, Adaptive Learning System

2011 ◽  
pp. 379-393
Author(s):  
Mei-Yu Chang ◽  
Wernhuar Tarng ◽  
Fu-Yu Shin
Keyword(s):  
Adaptive Learning ◽  
Learning System ◽  
Control Group ◽  
School Students ◽  
Web Based ◽  
Face To Face ◽  
Adaptive Learning System ◽  
Group B ◽  
Experimental Group ◽  
The Web

This study combined ideas from learning hierarchy and scaffolding theory to design a webbased, adaptive learning system to investigate the effectiveness of scaffolding for elementary school students having different levels of learning achievement. The topic chosen for learning was the Three States of Water. A quasi-experiment was conducted. In this experiment, students were divided into three groups: control group (without scaffolds), experimental group A (scaffolds providing by on-line conversation) and experimental group B (scaffolds providing by face-to-face conversation). The experimental results showed significant improvement for students after they had studied using the web-based, adaptive learning system. Specifically, scaffolds in the form of face-to-face conversations greatly enhanced the learning of high-achievement students. However, there were no significant differences between the low-achievement students with or without the provision of scaffolds. It was also discovered that the web-based, adaptive learning system could help students develop their learning responsibility.

Effectiveness of GSP-Aided Instruction

2015 ◽  
Vol 5 (2) ◽  
pp. 43-57
Author(s):  
Chin-Hsiu Tai ◽  
Shian Leou ◽  
Jeng-Fung Hung
Keyword(s):  
Formative Assessments ◽  
Superior Performance ◽  
Control Group ◽  
Traditional Lecture ◽  
Fifth Grade Students ◽  
Teaching Aids ◽  
Lecture Instruction ◽  
Group B ◽  
Difficulty Index ◽  
Experimental Group

The objective of this study was to determine the effectiveness of the Geometer's Sketchpad (GSP) in teaching the area of triangles to elementary students. The authors adopted a pretest-posttest research design with 2 equivalent groups. The subjects of the experiment comprised fifth-grade students from an elementary school in Pingtung County, divided into an experimental group of 26 students and a control group of 25 students. Both groups attended six 40-min lessons over a month, with the experimental group receiving GSP-aided instruction and the control group receiving traditional lecture instruction. The authors revised the test instrument based on , and the instrument was further reviewed and revised by experts. They conducted a third revision of the contents after performing a pilot test. The difficulty index of the test ranged between 0.18 and 1, and the discrimination index ranged between 0.13 and 0.88. The split-half reliability of the test was 0.8723. The results revealed the following: (a) in learning the area of triangles, the experimental group performed significantly better than the control group; (b) the results of the formative assessments indicated a superior performance in the experimental group compared with the control group in all of the lessons, with the exception of the second lesson; and (c) the male and female students in the experimental group and the control group exhibited no significant differences. Based on these results, the authors suggest that students be offered the opportunity to use concrete teaching aids or computer simulations when learning the area of triangles.

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