Comparative Assessment of the Anti-Microbial Properties of Artocarpus altilis, and Syzygium cumini Leaves against Staphylococcus aureus and Escherichia coli

Penelitian ini merupakan lanjutan dari penelitian sebelumnya tentang produksi asam galat dari limbah kulit buah lokal di Lombok. Limbah kulit buah lokal yang digunakan yaitu kulit buah kepundung (Baccaurea racemosa Muell.Arg), kulit buah juwet (Syzygium cumini), dan kulit buah manggis (Garcinia mangostana). Pada penelitian ini dilakukan pengujian asam galat yang dihasilkan, untuk mengetahui aktivitas antioksidan dan antibakteri. Pengujian sifat antioksidan dilakukan dengan menggunakan metode DPPH, sedangkan aktivitas antibakteri diuji dengan menggunakan metode difusi agar pada bakteri Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa asam galat dari ketiga kulit buah memiliki aktivitas antioksidan. Asam galat dari kulit buah kepundung, kulit buah juwet dan kulit buah manggis memiliki IC50 masing-masing sebesar 5.95; 5,96; dan 5,92 ppm. Berbeda dengan kemampuan sebagai antioksidan, asam galat resistan terhadap bakteri Escherichia coli dan Staphylococcus aureus yang menunjukkan bahwa asam galat tidak memiliki aktivitas sebagai antibakteri.Antibacterial and antioxidant activity of gallic acid from peel local fruit which produced by tannase. This research is a continuation of the previous research about the gallic acid production from peel fruit local in Lombok. The local fruit waste used are kepundung rind (Baccaurea racemosa Muell.Arg), juwet rind (Syzygium cumini) and mangosteen rind (Garcinia mangostana). In this research, the produced Gallic acid was tested to determine its antioxidant and antibacterial activity. The antioxidant test conducted by DPPH method, meanwhile, the antibacterial activity was performed by agar diffusion method to bacteria Escherichia coli and Staphylococcus aureus. The results show that the produce gallic acid from those three rind provide antioxidant activity. Gallic acid from kepundung rind, juwet rind, and mangosteen rind have IC50 values of 5,95; 5,96; and 5,92, respectively. Gallic acid not showed inhibitory actions against the growth of Escherichia coli and Staphylococcus aureus. This result show that gallic acid from local fruit waste did not provide an antibacterial activity.

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Comparative assessment of antibacterial activities of Syzygium aromaticum and Cyperus articulatus against Staphylococcus aureus and Escherichia coli

Journal of Pharmacy & Bioresources ◽

10.4314/jpb.v16i2.7 ◽

2019 ◽

Vol 16 (2) ◽

pp. 139

Author(s):

Muhammad Ali Dikwa ◽

Umar Aliyu Abdullahi ◽

Shina I. Sadiq ◽

Sunusi Ado Yahya ◽

Sunday Eghobor ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antibacterial Activities ◽

Comparative Assessment ◽

Syzygium Aromaticum

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UJI FITOKIMIA DAN DAYA HAMBAT EKSTRAK DAUN JUWET (Syzygium cumini) TERHADAP PERTUMBUHAN Escherichia coli DAN Staphylococcus aureus ATCC

SIMBIOSIS Journal of Biological Sciences ◽

10.24843/jsimbiosis.2017.v05.i02.p03 ◽

2017 ◽

Vol 5 (2) ◽

pp. 47 ◽

Cited By ~ 1

Author(s):

Kadek Sudarmi ◽

Ida Bagus Gede Darmayasa ◽

I Ketut Muksin

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Leaf Extract ◽

Syzygium Cumini ◽

E Coli ◽

Randomized Design ◽

Test Concentration ◽

Completely Randomized Design ◽

Difference Test ◽

Test Level

Bacterial Escherichia coli dan Staphylococcus aureus ATCCis a normal flora bacteria that naturally exist human body. This bacteria may by pathogenic if it exceeds acertain limits. To prevent the disease caused, need to be overcome by using herbal ingredients one of them is juwet leaves (Syzygiumcumini).The purpose of the study was to determine wheter the extract of juwet leaf (S. cumini) can inhibited the growth of E. coli and S. aureus ATCC and to know compounds contained in juwet leaf extract (S. cumini). This research used diffusion wells methodwith 6 treatments and 4 replications. Date analysis using completely randomized design (CRD) with using ANOVA variance analysis. If the date obtained has a real difference test level at the 5% (P? 0,5) that continue with Duncan test. Concentration of the extract tested was 0%, 5%, 10%, 15%, 25% and 50%. Juwet leaf extract is able to inhibited the growth of E. coli and S. aureus ATCC which is shown by the formation of clear zone. Concentration extract leaf which is effective to inhibit E. coli and S. aureus ATCC is the concentration 50% (18,9 mm) for E. coli and (16,5 mm) for S. aureus ATCC. Screening of phytochemical test of juwet leaf extract positive containing alkaloids, phenolic, steroids and saponins. Keywords : juwet leaf extract (Syzygium cumini), Escherichia coli, Staphylococcus aureus ATCC

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UJI DAYA HAMBAT PEWARNA ALAMI KULIT BATANG JAMBLANG (Syzygium cumini) TERHADAP BAKTERI Escherichia coli, Staphylococcus aureus DAN JAMUR Aspergilus niger

Indigenous Biologi : Jurnal Pendidikan dan Sains Biologi ◽

10.33323/indigenous.v3i3.91 ◽

2021 ◽

Vol 3 (3) ◽

pp. 128-136

Author(s):

Febriana Bani Utang ◽

Anggreini D.N Rupidara ◽

Alan Ch Sabuna

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Aspergillus Niger ◽

Inhibitory Activity ◽

Herbal Medicines ◽

Effective Concentration ◽

Natural Dyes ◽

Syzygium Cumini ◽

Food Ingredient ◽

E Coli

ABSTRACTJamblang (Syzygium cumini) belongs to the family of the guava tribe which has benefits as a food ingredient as well as herbal medicines. Jamblang bark produces tanners (tannins) to color the nets. The purpose of this study was to determine the inhibition of natural dyes of jamblang (Syzygium cumini) bark against Escherichia coli, Staphylococcus aureus, and Aspergillus niger fungi. The method used in this study wis an experimental method, which consists of 3 treatments of natual dyes of the bark cuminis where 40%, 60%, and 80% consentrations and 3 replicates. Data of the inhibitory activity where tested using paper disk method and was analyzed statistically using ANOVA test with SPSS 16.0. statistic aplication. The natural dyes from each concentration showed a inhibitoryactivity against E. coli, S. aureus, and A. niger. The effective concentration of inhibitory activity in E. Coli was 60%, natural dyes category zonas 4.0 mm or weak inhibitory activity The effective concentration of inhibitory activity in S. aureus was 40%, natural dyes category zonas was 7.0 mm or moderateinhibitory activity. The effective concentration of inhibitory activity in A. Niger was 80% natural dyes category zonas was 4.0 mm or weak inhibitory activity.Kaywords :Antibacterial test,Jamblang (Syzygiumcumini) bark, natural coloring Escherichia coli, Staphylococcus aureus, Aspergillus niger.

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CRIBADO FITOQUÍMICO BÁSICO Y PROPIEDADES ANTIBACTERIANAS, ANTIFÚNGICAS Y ANTIOXIDANTES DE SYZYGIUM CUMINI, UN ÁRBOL DE GUYANA

Revista Boliviana de Química - Vol. 36, No. 1 2019, Abril 30 ◽

10.34098/2078-3949.37.3.1 ◽

2020 ◽

Vol 37 (3) ◽

Author(s):

Brij Bhushan Tewar ◽

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Candida Albicans ◽

Syzygium Cumini

Syzygium cumini o Jamun es un árbol tropical de hoja perenne de la familia de plantas ornamentales Myrtaceae. El material vegetal (hojas) de Syzygium cumini (Jamun) se colectó en el Instituto de Ciencia y Tecnología Aplicadas (IAST) de la Universidad de Guyana, campus de Turkeyen. Las hojas se secaron en estufa a 50-60 ºC durante 72 h. Se calculó el contenido de humedad. Las hojas secas se trituraron y se extrajeron en etanol, metanol, acetato de etilo y disolventes de cloroformo, sucesivamente. Se recogieron los extractos y se evaporó el disolvente en rotavapor. Se añadió el disolvente respectivo al extracto líquido semisólido viscoso para completar el volumen deseado de solución de extracto. Los microorganismos (Escherichia coli, Staphylococcus aureus y Candida albicans) se obtuvieron de GPHC, Georgetown, Guyana. La actividad antioxidante, antimicrobiana y antifúngica de los diferentes extractos se evaluó mediante métodos descritos en la literatura. El poder antioxidante máximo y mínimo fue exhibido por los extractos de metanol y cloroformo, respectivamente. Se encontró que los extractos de cloroformo y acetato de etilo tenían la actividad antibacteriana máxima y mínima contra Escherichia coli, Staphylococcus aureus, así como la actividad antifúngica contra Candida albicans, respectivamente, por el método de difusión por disco. El análisis fitoquímico de los extractos de hojas de Syzygium cumini reveló la presencia de carbohidratos, terpenoides, proteínas, aminoácidos y flavonoides.

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Aktivitas Antibakteri dan Antioksidan Asam Galat dari Kulit Buah Lokal yang Diproduksi dengan Tanase

ALCHEMY Jurnal Penelitian Kimia ◽

10.20961/alchemy.14.1.11300.131-142 ◽

2018 ◽

Vol 14 (1) ◽

pp. 131

Author(s):

Eka Junaidi ◽

Yunita Arian Sani Anwar

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antioxidant Activity ◽

Antibacterial Activity ◽

Gallic Acid ◽

Diffusion Method ◽

Syzygium Cumini ◽

Garcinia Mangostana ◽

Dpph Method ◽

Fruit Waste

Penelitian ini mempelajari tentang produksi asam galat menggunakan enzim tanase dari limbah kulit buah lokal di Lombok. Limbah kulit buah lokal yang digunakan yaitu kulit buah kepundung (Baccaurea racemosa Muell.Arg), kulit buah juwet (Syzygium cumini), dan kulit buah manggis (Garcinia mangostana). Pada penelitian ini dilakukan pengujian asam galat yang dihasilkan, untuk mengetahui aktivitas antioksidan dan antibakteri. Pengujian sifat antioksidan dilakukan dengan menggunakan metode DPPH, sedangkan aktivitas antibakteri diuji dengan menggunakan metode difusi agar pada bakteri Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa asam galat dari ketiga kulit buah memiliki aktivitas antioksidan. Asam galat dari kulit buah kepundung, kulit buah juwet dan kulit buah manggis memiliki IC50 masing-masing sebesar 5.95; 5,96; dan 5,92 ppm. Berbeda dengan kemampuan sebagai antioksidan, asam galat resistan terhadap bakteri Escherichia coli dan Staphylococcus aureus yang menunjukkan bahwa asam galat tidak memiliki aktivitas sebagai antibakteri.Antibacterial and Antioxidant Activity of Gallic Acid Extracted from Local Fruit Peels Produced by Tannase. This research studied the gallic acid production using tannase enzym from fruit local peels in Lombok. The local fruit waste used are kepundung rind (Baccaurea racemosa Muell.Arg), juwet rind (Syzygium cumini) and mangosteen rind (Garcinia mangostana). The produced Gallic acid was tested to determine its antioxidant and antibacterial activity. The antioxidant test conducted by DPPH method, meanwhile, the antibacterial activity was performed by agar diffusion method against bacteria Escherichia coli and Staphylococcus aureus. The results showed that the produce gallic acid from those three rind provide antioxidant activity. Gallic acid from kepundung rind, juwet rind, and mangosteen rind have IC50 values of 5,95; 5,96; and 5,92, respectively. Gallic acid does not showed inhibitory actions against the growth of Escherichia coli and Staphylococcus aureus. This result showed that gallic acid from local fruit waste provided no an antibacterial activity.

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The Effectiveness of Ethyl Acetate Extract From Breadfrui (Artocarpus Altilis) Leaves to Inhibit Diarrhea-Causing Bacteria

Biomedika ◽

10.31001/biomedika.v14i1.869 ◽

2021 ◽

Vol 14 (1) ◽

pp. 29-35

Author(s):

Yusianti Silviani ◽

Ardy Prian Nirwana

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Artocarpus Altilis ◽

Inhibition Zones ◽

And Staphylococcus Aureus

Diarrhea is a health problem that commonly occurs in developing countries. Bacteria that cause diarrhea are among others Escherichia coli, Salmonella typhosa and Staphylococcus aureus. This research aimed to investigate the diameters of inhibition zones of breadfruit leaf (A. altilis) ethyl acetate extract in different concentrations against the growth of Escherichia coli, Salmonella typhosa and Staphylococcus aureus. The research applied an experimental laboratory by using a post-test control group design. This research was performed at the Bacteriology Laboratory of STIKES Nasional by using the diffusion disk method. The research showed the radical zone diameters against Escherichia coli with the concentrations of 20%, 40%, 60%, 80%, and 100%, were 6.16 mm, 6.41 mm, 6.74 mm, 7.49 mm, and 7.79 mm, respectively. The inhibition zones against Staphylococcus aureus were 8.15, mm 9.43 mm, 10.29, 10.38 mm and 11.42 mm, while against Salmonella typhosa were 7.94 mm, 8.87 mm, 10.15 mm, 10.26 mm, and 11.23 mm, respectively. The results of the ANOVA test showed the p-value=0.00 and the results of the LSD test revealed the differences in the inhibition effects of A. altilis leaf extract against the growth of E. coli, S. typhosa and S. aureus. This study concludes that concentration variations of Artocarpus altilis leaf ethyl acetate extract can inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhosa.

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ESTUDO FITOQUÍMICO, BOTÂNICO E DAS PROPRIEDADES ANTIMICROBIANAS DE Pterocaulon interruptum DC. (ASTERACEAE)

Visão Acadêmica ◽

10.5380/acd.v3i2.514 ◽

2002 ◽

Vol 3 (2) ◽

Author(s):

A. C. W. HEEMANN ◽

O. G. MIGUEL

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Essential Oil ◽

Methyl Ether ◽

Alcoholic Extract ◽

Microbial Properties ◽

Aerial Parts ◽

Phytochemical Studies ◽

Asteraceae Family

No presente trabalho foram realizados estudos sobre a espécie Pterocaulon interruptum DC., Asteraceae, cujas partes aéreas foram submetidas a estudo fitoquímico, sendo o processo extrativo por Soxhlet, o qual apresenta melhor rendimento e rapidez, isolando-se e caracterizando-se cinco compostos: sabandinol (cumarina), quercetina (flavonol), taxifolina 7-O-prenilada (di-hidroflavonol), estigmasterol (esteróide) e 3-O-acetil taraxasterol (esteróide). Do óleo essencial foram caracterizados oito compostos: a-tujeno; 1,4,6-trimetil-5,6-dihidronaftaleno; 3-heptanona; acetaldeído benzênico; alil cilohexano; carvacrol (metil éter); orto-cimeno e safrol. Estudos botânicos foram realizados para determinação das características anatômicas e possível comparação com estudos anteriores. A fração do extrato alcoólico avaliada não apresentou atividade inibitória frente a cepas de Escherichia coli, Pseudomonas aeruginosa e Staphylococcus aureus. PHYTOCHEMICAL, BOTANICAL STUDIES AND ANTI MICROBIAL PROPERTIES FROM Pterocaulon interruptum DC. (ASTERACEAE) Abstract In the present paper was realized studies about Pterocaulon interruptum DC., Asteraceae family, which aerial parts were submitted to botanical study to obtain anatomic characteristics and possibility to compare with others studies. It was conducted the phytochemical studies and the extractive process was developed by Soxhlet, which presented a better performance and speed. It was isolated five compounds: sabandinol (coumarin), quercetin (flavonol), 7-O-prenyl taxifolin (dihydroxyflavonol), stigmasterol (steroid) and 3-O-acetyl taraxasterol (steroid). From essential oil was identified eight compounds: a-thujene; 1,4,6-trimethyl-5,6-dihydronaphtalene; 3-heptanone; benzenic acetaldeid; alil ciclohexane; carvacrol (methyl ether); ortho-cimene and safrol. The fraction available from alcoholic extract wasn´t active like anti microbial in the microorganisms species Escherichia coli, Pseudomonas aeruginosa e Staphylococcus aureus.

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Aktivitas Antibakteri Ekstrak Etanol Daun Sukun (Artocarpus altilis) Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli

Pharmacon Jurnal Farmasi Indonesia ◽

10.23917/pharmacon.v0i0.10108 ◽

2020 ◽

pp. 10-20

Author(s):

Fuan Maharani Fiana ◽

Naelaz Zukhruf Wakhidatul Kiromah ◽

Ery Purwanti

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Leaf Extract ◽

Ethanol Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Positive Control ◽

Negative Control ◽

Artocarpus Altilis ◽

Escherichia Coli Bacteria

Diarrhea is caused by bacterial infections, especially Staphylococcus aureus and Escherichia coli. The breadfruit leaves (Artocarpus altilis) are known contain flavonoid compounds, alkaloids, saponins, and tannins which can be used as antibacterial. This research was conducted to determine the ethanol extract of the breadfruit leaves against Staphylococcus aureus and Escherichia coli bacteria. The manufacture of the breadfruit leaf extract was using a maceration method with 96% ethanol solvent. The result of the tube test and the TLC test was positive, the breadfruit leaf extract containing flavonoids and tannins which have potential as antibacterial. The antibacterial test was carried out using the disc diffusion method (Kirby-Bauer test) against Staphylococcus aureus and Escherichia coli bacteria incubated for 1 x 24 hours. The extract concentrations tested were 10%, 15% and 20%. The positive control was using erythromycin antibiotics and the negative control was using aquades. The identification of the chemical content of plants is done by tube test and the TLC test. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Staphylococcus aureus bacteria, the average diameter of inhibitory zones respectively were 3.67, 3.50 and 2.67 mm with the positive control diameter of inhibition zone was 18.5 mm, the negative control diameter of inhibition zone was 0 mm. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Escherichia coli bacteria, the average diameter of inhibitory zones respectively were 5.33, 3.17 and 3.33 mm with the positive control inhibition zone diameter of 28.5 mm and the negative control of inhibition zone diameter of 0 mm. The activity of ethanol extract of the breadfruit leaves against the Staphylococcus aureus and Escherichia coli bacteria is included in the weak category.

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Antimicrobial and cytotoxic activity of Macrophomina phaseolina isolated from gummosis infected citrus reticulata

Chittagong University Journal of Biological Sciences ◽

10.3329/cujbs.v5i1.13378 ◽

2013 ◽

pp. 125-133

Author(s):

Rubal C Das ◽

Rajib Banik ◽

Robiul Hasan Bhuiyan ◽

Md Golam Kabir

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Brine Shrimp ◽

Inhibitory Concentration ◽

Lethal Dose ◽

Macrophomina Phaseolina ◽

Chloroform Extract ◽

Citrus Reticulata ◽

Gram Negative Bacteria ◽

Gram Negative

Macrophomina phaseolina is one of the pathogenic organisms of gummosis disease of orange tree (Citrus reticulata). The pathogen was identified from the observation of their colony size, shape, colour, mycelium, conidiophore, conidia, hyaline, spore, and appressoria in the PDA culture. The crude chloroform extracts from the organism showed antibacterial activity against a number of Gram positive and Gram-negative bacteria. The crude chloroform extract also showed promising antifungal activity against three species of the genus Aspergillus. The minimum inhibitory concentration (MIC) of the crude chloroform extract from M. phaseolina against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Shigella sonnie were 128 ?gm, 256 ?gm, 128 ?gm and 64 ?gm/ml respectively. The LD50 (lethal dose) values of the cytotoxicity assay over brine shrimp of the crude chloroform extract from M. phaseolina was found to be 51.79 ?gm/ml. DOI: http://dx.doi.org/10.3329/cujbs.v5i1.13378 The Chittagong Univ. J. B. Sci.,Vol. 5(1 &2):125-133, 2010

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