Antifungal and antibacterial activities of polyherbal toothpaste against oral pathogens, In vitro

Batool Sadeghi-Nejad; Eskandar Moghimipour; Sedigheh Yusef Naanaie; Shahrzad Nezarat

doi:10.18502/cmm.4.2.65

Antifungal and antibacterial activities of polyherbal toothpaste against oral pathogens, In vitro

Current Medical Mycology ◽

10.18502/cmm.4.2.65 ◽

2018 ◽

Vol 4 (2) ◽

Cited By ~ 1

Author(s):

Batool Sadeghi-Nejad ◽

Eskandar Moghimipour ◽

Sedigheh Yusef Naanaie ◽

Shahrzad Nezarat

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Oral Hygiene ◽

Periodontal Diseases ◽

Inhibition Zone ◽

Diffusion Method ◽

Leaf Extracts ◽

Oral Infections ◽

Oral Pathogens

Original article Article History: Received: 23 April 2018 Revised: 20 July 2018 Accepted: 06 August 2018 * Corresponding author:Batool Sadeghi-NejadAbadan School of Medical Sciences, Abadan, Iran.Email: [email protected] Introduction One of the most common chronic oral infections in the world is dental caries [1]. Oral pathogenic microorganisms have been the cause of dental plaques, dental caries, as well as gingival and periodontal diseases [2]. Streptococcus mutans is one of the main opportunistic pathogens of dental caries, which is responsible for the formation of dental plaque and caries [3]. Other microorganisms associated with this oral condition include Escherichia coli, S. aureus [4], and Candida species. C. albicans is the most frequent yeast isolated from the oral cavities with poor oral hygiene [2]. The formulation ingredients of commercial toothpastes are mostly chemical substances, such as fluoride and whitening agents [2]. The literature contains evidence regarding the adverse effects of fluoride and bleaching agents (e.g., peroxide-based agents) used in the commercial toothpastes [5]. With this background in mind, the present study aimed to formulate a polyherbal toothpaste without any A B S T RA C TBackground and Purpose: Herbal toothpastes are more secure and efficacious and less poisonous due to containing natural chemicals as compared with the synthetic toothpastes. The present study aimed to formulate a polyherbal toothpaste using accessible medicinal plants in Iran and evaluate its efficiency in the protection of oral hygiene and prevention of dental caries. Materials and Methods: The developed toothpaste was made of the leaf extracts of Artemisia dracunculus, Satureja khuzestanica (Jamzad), and Myrtus communis (Linn), combined at four different dilutions, namely 1:4 (25%), 1:1 (50%), 3:4 (75%), and (100%), with sterile distilled water. The product was tested against five microorganisms, including Streptococcus mutans, Lactobaccilus caseie, S. sanguis, S. salivarius, and Candida albicans, using agar well diffusion method. Results: After 24 h of incubation, the maximum mean diameters of inhibition zone against L. caseie and C. albicans were obtained as 17-30 and 10-25 mm, respectively. Furthermore, the minimum mean diameter of inhibition zone against S. salivarious was estimated as 15-20 mm. Conclusion: The formulated toothpaste showed potent inhibitory activities against Gram-positive bacteria and C. albicans. Therefore, more studies are required to accurately investigate the efficacy of the formulated toothpaste. Keywords: Antibacterial, Antifungal, Oral pathogens, Polyherbal toothpaste, Yeast

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The PHYTOCHEMICALS, ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Senna alata AND Senna tora LEAF EXTRACTS AGAINST BACTERIAL STRAINS CAUSING SKIN INFECTIONS

Bacterial Empire ◽

10.36547/be.2019.2.1.19-25 ◽

2019 ◽

Vol 2 (1) ◽

pp. 19

Author(s):

Murni Halim

Keyword(s):

Leaf Extract ◽

Antimicrobial Properties ◽

Inhibition Zone ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Bacterial Strains ◽

Leaf Extracts ◽

Natural Drugs ◽

Senna Alata

A study was carried out to screen for phytochemical constituents and assess the antioxidant and antimicrobial activities of Senna alata and Senna tora leaf extracts. The leaves were first dried at room temperature and 50°C in an oven prior to solvent extraction using ethanol and methanol. The in-vitro qualitative assays showed that both S. alata and S. tora leaf extracts contained bioactive and secondary metabolites components such as tannins, steroids, saponin, terpenoids, glycosides, flavonoids and phenols. The antioxidant activity and capacity test were carried out by conducting free radical of 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity and Ferric reduction antioxidant plasma (FRAP) assays. Both assays showed S. tora leaf extract has higher antioxidant capacity than S. alata leaf extract. The efficacy of these leaf extracts were tested against skin pathogens through agar well diffusion method. S. alata extract showed an inhibition zone (1.15 – 1.59 mm) against Pseudomonas aeruginosa while S. tora extracts exhibited a strong antimicrobial activity against S. epidermidis (inhibition zone of 12 – 16.94 mm) followed by P. aeruginosa (inhibition zone of 1 – 1.59 mm). Nonetheless, no inhibition zone was observed for S. aureus by both leaf extracts. The phytochemicals and antioxidant constituents as well as inhibitory potential on skin pathogens possessed by S. alata and S. tora leave highlighted their potential utilization in the development of natural drugs or cosmetics to treat skin related diseases or infections.

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In Vitro Antimicrobial Activity of Zodia (Evodia suaveolens) Leaf Extract on Pathogenic Agents Dragon Fruit Plant

Jurnal Biota ◽

10.19109/10.19109/biota.v6i2.6236 ◽

2020 ◽

Vol 6 (2) ◽

pp. 78-85

Author(s):

Trio Ageng Prayitno ◽

Nuril Hidayati

Keyword(s):

Antimicrobial Activity ◽

Leaf Extract ◽

Plant Pathogens ◽

Inhibition Zone ◽

Diffusion Method ◽

Leaf Extracts ◽

Dragon Fruit ◽

Fruit Plants ◽

Pathogenic Agents

The use of antimicrobials from plant extracts has not been used optimally to control pathogenic agents in dragon fruit plants. The purpose of this research was to determine the antimicrobial activity of zodia (Evodia suaveolens) leaf extracts on pathogenic agents of dragon fruit plants in vitro. The research method is laboratory research with Completely Randomized Design (CRD). The antimicrobial concentrations of zodia (E. suaveolens) leaf extract used six types including 50%, 60%, 70%, 80%, 90%, and 100% with four replications. The research sample was the leaf of zodia (E. suaveolens), Pseudomonas aeruginosa and Fusarium oxysporum strain Malang. Test the antimicrobial activity of zodia (E. suaveolens) leaf extracts on the growth of P. aeruginosa and F. oxysporum using the disc-diffusion method and wells method. The research instrument was used the observation sheet of the diameter of inhibition zone indicated by the clear zone. The diameter of inhibition zone data were analyzed using the One Way ANOVA test. The results showed that the antimicrobial activity of zodia (E. suaveolens) leaf extract significantly inhibited the growth of P. aeruginosa and F. oxysporum (P <0.05). These results recommend zodia (E. suaveolens) leaf extract as an antimicrobial agent for dragon fruit plant pathogens.

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Antibacterial Activity Of Tangerine Powder (Citrus reticulata Blanco.) Against Caries-Causing Bacteria (Streptococcus mutans)

FITOFARMAKA: Jurnal Ilmiah Farmasi ◽

10.33751/jf.v10i2.2198 ◽

2020 ◽

Vol 10 (2) ◽

pp. 148-157

Author(s):

Framesti Frisma Sriarumtias ◽

Aji Najihudin ◽

Nopi Rantika ◽

Rita Nengsih

Keyword(s):

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Dental Caries ◽

Streptococcus Mutans ◽

Inhibitory Concentration ◽

Inhibition Zone ◽

Diffusion Method ◽

Pharmaceutical Ingredients ◽

Caries Treatment ◽

Inhibitory Power

Dental caries is caused by Streptococcus mutans, which lives in the oral cavity and causes plaque on the teeth. Dental caries treatment involves restoration or filling of tooth tissue that has been perforated due to dental caries. The purpose of this study was analyze the effectivity of tangerine juice powder in preventing the emergence of dental caries caused by Streptococcus mutans. The experimental method was used in this study started by making a powder from tangerine jiuce using freeze drying method, followed by testing the antibacterial activity using the disc diffusion method. In addition, the minimum inhibitory concentration (MIC) was also sought by measuring the diameter of the clear zone. The results showed that tangerine powder has antibacterial activity, with a minimum inhibitory concentration of 10%. The highest activity of tangerine powder to inhibit the growth of Streptococcus mutans bacteria was observed at concentration of 25% with an inhibition zone diameter of 15.8 mm, and is classified as having moderate inhibitory power. According to the result of this study is that tangerine juice powder has a good antibacterial activity against Streptococcus mutans and can be developed into a pharmaceutical ingredients to prevent dental caries.

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ACTIVITY OF PERICARP EXTRACT OF MANGOSTEEN AGAINST ORAL STREPTOCOCCI

Dentika Dental Journal ◽

10.32734/dentika.v20i1.739 ◽

2017 ◽

Vol 20 (1) ◽

pp. 41-46

Author(s):

Dewi Nurul Mustaqimah ◽

Josh Erry HW

Keyword(s):

Antimicrobial Activity ◽

Dental Caries ◽

Streptococcus Mutans ◽

Antimicrobial Agents ◽

Biological Activities ◽

World Health ◽

Direct Result ◽

Oral Streptococci ◽

Oral Pathogens

The increasing prevalence of dental caries is still as a major world health problem. Caries is the direct result of acid production by cariogenic oral pathogens, especially Streptococcus mutans. New and better antimicrobial agents active against cariogenic bacteria with minimal side effects on the oral tissues are much needed, especially natural agents derived directly from plants. Phytochemical studies have shown that the extracts from various parts of mangosteen or Garciniamangostana Linn tree contain varieties of secondary metabolites such as prenylated and oxygenated xanthones, many of which have been found in vitro to have antimicrobial properties against oral pathogens. Several studies which examined the eficacy of herbal for human health have shown that xanthones from mangosteen have remarkable biological activities such as antioxidant, antimicrobial, anticancer etc, and had no cytotoxic effects on human gingival fibroblasts. Their results showed that among these xanthone derivatives obtain from pericarp extract of mangosteen, α-mangostin has the most potent antimicrobial activity against cariogenic Streptococcus mutans. It can be concluded that the strong antimicrobial activity of the pericarp extract of mangosteen is a good drug of choice that might be helpful in preventing the dental caries.

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Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro

Jurnal Kesehatan ◽

10.26630/jk.v9i3.967 ◽

2018 ◽

Vol 9 (3) ◽

pp. 336

Author(s):

Ni Luh Budi Artaningsih ◽

Nur Habibah ◽

Mastra Nyoman

Keyword(s):

Antibacterial Activity ◽

Streptococcus Mutans ◽

Ethanol Extract ◽

Inhibition Zone ◽

Gliricidia Sepium ◽

Disk Diffusion ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Growth Inhibition Zone

Streptococcus mutans is a positive gram bacteria which cause dental caries. From the several previous studies, Gamal leaf has been predicted as the antibacterial agent because of their active substance such as tannins, alkaloids, saponins, and flavonoids. The objective of the study was to know the antibacterial activity of ethanol extract of Gamal leaf (Gliricidia sepium) for Streptococcus mutans in various concentrations. This study was a true experimental with post-test only control design. The determination of antibacterial activity in this study was conducted by using Kirby Bauer disk diffusion method with the various concentrations, there were 40, 50, 60, 70 and 80%. The positive and negative controls in this study were 30 µg disk diffusion of chloramphenicol and the 96% of ethanol. The average inhibition of zone diameter in the concentrations of 40, 50, 60, 70 and 80% were 11.3, 12.3, 13.4, 15.3 and 19.2 mm, respectively. One Way ANOVA statistical analysis showed that the value of p was 0.000, so there was a difference of growth inhibition zone of Streptococcus mutans in various concentrations of ethanol extract of Gamal leaf (Gliricidia sepium). Based on the result, it can be concluded that the ethanol extract of Gamal leaf (Gliricidia sepium) have strong antibacterial activity, especially to Streptococcus mutans bacteria.

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The Difference of Antibacterial Power between Cocoa Peel (Theobroma Cacao L.) Extract 6% compared to Chlorhexidine Digluconate 2% Againts Streptococcus mutans (In vitro)

Conservative Dentistry Journal ◽

10.20473/cdj.v11i1.2021.1-5 ◽

2021 ◽

Vol 11 (1) ◽

pp. 1

Author(s):

Alissa Amanda ◽

Tamara Yuanita ◽

Galih Sampoerno

Keyword(s):

Streptococcus Mutans ◽

Theobroma Cacao ◽

Inhibition Zone ◽

Diffusion Method ◽

Chlorhexidine Digluconate ◽

Significant Difference ◽

The Difference ◽

Theobroma Cacao L ◽

Peel Extract

Background: Before restoration, it is necessary to clean the cavity from the smear layer and residual bacteria such as Sreptococcus mutans using a 'gold standard' cavity cleanser, namely 2% Chlorhexidine digluconate (CHX), however CHX 2% has a disadvantage of having a toxic effect on fibroblasts, osteoblasts, myoblasts, odontoblast-like cells, Chinese hamster ovary cells, and buccal epithelial cells. The shortcomings of the 2% CHX triggered researchers to look for alternative cavity cleansers that are more biocompatible, namely cocoa peel extract because it contains of antibacterial compounds including alkaloids, flavonoids, tannins, saponins, and terponoids with a non-toxic 6% concentration. Purpose: To analyze the difference of antibacterial activity between cocoa peel extract with a concentration of 6% compared to chlorhexidine digluconate 2% against Streptococcus mutans. Methods: This research was an in vitro laboratory experimental study with the posttest only control group design which included two treatment groups, namely 6% cocoa peel extract and 2% CHX. This research was conducted using the inhibition zone diffusion method against S. mutans to see the antibacterial power of each sample. Results: There was a significant difference (p <0.05) in the mean diameter of the inhibition zone between 6% cacao peel extract, namely 11.5406 mm and CHX 2%, namely 13.2156 mm. Conclusion: Chlorhexidine digluconate 2% has a greater antibacterial power than 6% cocoa peel extract (Theobroma cacao L.) against Streptococcus mutans.

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The Effectiveness Of Anchovy Concentration (Stolephorus insularis) as Antimicrobial to Streptococcus mutans (In Vitro)

DENTA ◽

10.30649/denta.v10i1.37 ◽

2016 ◽

Vol 10 (1) ◽

pp. 71

Author(s):

Almira Fa’Izah ◽

Istien Wardani ◽

Diana Soesilo

Keyword(s):

Dental Caries ◽

Streptococcus Mutans ◽

Positive Control ◽

Negative Control ◽

Diffusion Method ◽

Control Group ◽

Oral Diseases ◽

Clear Zone ◽

Control Group Design

Background: Dental and oral diseases which are often found in children is dental caries. Streptococcus mutans is the main cause of caries. Caries can be prevented by using a topical application of fluoride. The Anchovy (Stolephorus insularis) contains protein, vitamins (A, B1, C), and minerals (Fe, Ca, K, F). Calcium fluoride (CaF2) within the anchovy can inhibit the occurrence of dental caries. Purpose: The aim of this study was to determine the antimicrobial ability of anchovy extract (Stolephorus insularis) to Streptococcus mutans. Materials and Methods: This study was a laboratory experimental research with post test only control group design. Diffusion method were applied with 2 controls: negative control used DMSO 1%, positive control used NaF solution, and 3 concentrations of anchovy extract (Stolephorus insularis) 3%, 6%, and 12%, each group were composed of 6 samples. Antimicrobial was assessed by measuring the diameter of the clear zone around the discs contained the anchovy extract (Stolephorus insularis). Data were analyzed by Kruskal-Wallis test followed by Mann-Whitney test. Result: The results from this study showed clear zone around the discs of the anchovy extract (Stolephorus insularis). The more concentration of the extract showed the more antimirobial zone diameter. The average zone of antimicrobial at the concentration of 3% were 7,11 mm, 6% 9,5 mm, 12% 10,78 mm, for the negative control DMSO 1% 6 mm and the positive control NaF solution 8,16 mm. The largest diameter of the clear zone was at concentration of 12% (P < 0,05). Conclusion: The anchovy extract (Stolephorus insularis) had an antimicrobial effect to the growth of Streptococcus mutans.

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Microbiological Assessment of Moringa Oleifera Extracts and Its Incorporation in Novel Dental Remedies against Some Oral Pathogens

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2016.132 ◽

2016 ◽

Vol 4 (4) ◽

pp. 585-590 ◽

Cited By ~ 12

Author(s):

Hanaa Elgamily ◽

Amani Moussa ◽

Asmaa Elboraey ◽

Hoda EL-Sayed ◽

Marwa Al-Moghazy ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Candida Albicans ◽

Streptococcus Mutans ◽

Moringa Oleifera ◽

Inhibition Zone ◽

Extraction Methods ◽

Diffusion Method ◽

Oral Pathogens ◽

Ethanol Extracts ◽

Different Parts

AIM: To assess the antibacterial and antifungal potentials of different parts of Moringa oleifera plant using different extraction methods in attempts to formulate natural dental remedies from this plant.MATERIAL AND METHODS: Three solvents extracts (Ethanol, acetone, and ethyl acetate) of different parts of Egyptian Moringa tree were prepared and tested against oral pathogens: Staphylococcus aureus, Streptococcus mutans, and Candida albicans using disc diffusion method; As well as to incorporate the plant extract to formulate experimental toothpaste and mouthwash. The two dental remedies were assessed against the same microbial strains. Statistical analysis was performed using One-Way ANOVA test to compare the inhibition zone diameter and t-test.RESULTS: Ethanol extracts as well as leaves extracts demonstrated the highest significant mean inhibition zone values (P ≤ 0.05) against Staphylococcus aureus and Streptococcus mutans growth. However, all extracts revealed no inhibition zone against Candida albicans. For dental remedies, experimental toothpaste exhibited higher mean inhibition than the mouthwash against Staphylococcus aureus, Streptococcus mutans and only the toothpaste revealed antifungal effect against Candida albicans.CONCLUSION: The different extracts of different parts of Moringa showed an antibacterial effect against Staphylococcus aureus and Streptococcus mutans growth. The novel toothpaste of ethanolic leaves extract has antimicrobial and antifungal potential effects all selected strains.

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Uji Aktivitas Antibakteri Ekstrak Etanol Daun Kamboja (Plumeria Sp) Terhadap Bakteri Escherichia coli Secara In Vitro

Edu Masda Journal ◽

10.52118/edumasda.v1i1.45 ◽

2017 ◽

Vol 1 (1) ◽

pp. 74

Author(s):

Susanti Erikania ◽

Yetty Hariningsih

Keyword(s):

Escherichia Coli ◽

Treatment Group ◽

Streptococcus Mutans ◽

Ethanol Extract ◽

Inhibition Zone ◽

Positive Control ◽

Nutrient Agar ◽

Diffusion Method ◽

Negative Controls

Tanaman obat menjadi sumber utama ditemukannya senyawa kimia baru yang memiliki khasiat secara farmakologi, salah satunya adalah tanaman kamboja (Plumeria sp). Tanaman kamboja adalah salah satu tanaman tradisional yang dilaporkan mempunyai berbagai khasiat, antara lain daunnya sebagai pencahar, antigatal dan antibakteri, buah dan kulit batangnya dilaporkan berefek antiinflamasi. Tujuan dari penelitian ini adalah mengetahui aktivitas daun kamboja sebagai antibakteri terhadap Escherichia coli secara in vitro. Penelitian ini menggunakan metode difusi cakram dengan 5 kelompok perlakuan meliputi kontrol negatif yaitu DMSO dengan konsentrasi 10 %, kontrol positif yaitu kloramfenikol konsentrasi 30 µg/disk dan ekstrak etanol daun kamboja (Plumeria sp) masing-masing sebesar 25 %, 50 % dan 100 %. Media yang digunakan adalah media nutrient agar (NA). Media steril yang telah diinokulasi dengan Escherichia coli selanjutnya diberi perlakuan dengan kertas cakram yang terdiri dari kontrol negatif, kontrol positif dan sampel, kemudian diinkubasi pada suhu 37°C selama 24 jam. Selanjutnya dilakukan pengukuran zona hambat menggunakan jangka sorong. Penelitian menunjukkan adanya zona hambat pada kelompok perlakuan ekstrak etanol daun kamboja pada konsentrasi 25%, 50%, 100 % dan kontrol positif, dan tidak tedapat zona hambat pada kelompok perlakuan dengan kontrol negatif. Ekstrak etanol daun kamboja mampu menghambat pertumbuhan Escherichia coli dengan daya hambat minimal adalah konsentrasi 25% yaitu menghasilkan zona hambat sebesar 7,13 mm, konsentrasi 50 % menghasilkan zona hambat sebesar 10,17 mm dan konsentrasi 100% memiliki nilai zona hambat paling besar yaitu 14,50 mm. Kata Kunci : Ekstrak etanol daun kamboja, Metode difusi cakram, Streptococcus mutans, Uji aktivitas antibakteri ABSTRACTMedicinal plants are the main source of the discovery of new chemical compounds that have pharmacological properties, one of which is frangipani (Plumeria sp). Frangipani plants are one of the traditional plants that are reported to have various benefits, including laxative, and antibacterial leaves, fruit and bark reported to have anti-inflammatory effects. The purpose of this study was to determine the activity of frangipani leaves as an antibacterial against Escherichia coli in vitro. This study uses a disk diffusion method with 5 treatment groups including negative control, namely DMSO with a concentration of 10%, positive control that is chloramphenicol concentration of 30 µg / disk and ethanol extract of frangipani leaves (Plumeria sp) each by 25%, 50% and 100% . The media used is nutrient agar (NA). Sterile media that have been inoculated with Escherichia coli are then treated with disc paper consisting of negative controls, positive controls and samples, then incubated at 37 ° C for 24 hours. Furthermore, inhibition zone measurements were made using calipers. The study showed that there were inhibitory zones in the treatment group of frangipani leaf ethanol extract at concentrations of 25%, 50%, 100% and positive control, and there were no inhibitory zones in the treatment group with negative controls. Ethanol extract of frangipani leaves can inhibit the growth of Escherichia coli with a minimum inhibitory concentration of 25% which produces a inhibition zone of 7.13 mm, a concentration of 50% produces an inhibitory zone of 10.17 mm and a concentration of 100% has the greatest inhibition zone value of 14 50 mm. Keywords: Frangipani leaf ethanol extract, Disc diffusion method, Streptococcus mutans, activity test antibacterial

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Effectiveness of Chinese Leaf Extract and Leaf Plants (Jatropha Multifida L.) As Antibacterial to Bacterial Growth Staphylococcus Aureus In Vitro

Window of Health Jurnal Kesehatan ◽

10.33368/woh.v0i0.13 ◽

2018 ◽

pp. 29-33

Author(s):

Dwi Anggita ◽

Yusriani Yusriani ◽

Dian Amelia Abdi ◽

Vivin Desiani

Keyword(s):

Staphylococcus Aureus ◽

Leaf Extract ◽

Average Diameter ◽

Inhibition Zone ◽

Positive Control ◽

Diffusion Method ◽

Leaf Extracts ◽

The Difference ◽

Jatropha Multifida

Jatropha multifida L. contains alkaloids, tannins, flavonoids, saponins and phenolic acids that differ from each part of the plant and the content of these substances is what makes L. multifida Jatropha has a function as an antibacterial so that the leaf extract and the gap of chinese distance ( Jatropha multifida L.) allegedly capable of inhibiting the growth of Staphylococcus aureus bacteria. The aim of this research was to know the effectivity of leaf extract and gum of Jatropha multifida L. to the growth of Staphylococcus aureus bacteria in vitro.Penelitian used true experimental post test design. Using disc diffusion method with concentration 25; 50; 75; and 100% v / v leaf and gum china distance. Positive control was used Clindamycin 5μg drip antibiotic paper disc. The data were analyzed descriptively. The result of the research showed the difference of different inhibition zone between treatments. In chinese leaf extracts showed different mean diameters, at concentrations of 25%, 50%, 75% with inhibit zone formed respectively 0 mm, 9.32 mm, and 17.48 mm and the highest inhibition zone at concentration of 100% with an average diameter of 22.24 mm. In the gap of chinese distance showed a different mean diameter, at concentrations of 25%, 50%, 75% with the inhibit zone formed respectively 16.08 mm, 18.15 mm, and 18.63 mm and the highest inhibition zone at a concentration of 100% with an average diameter of 21.91 mm. Clindamycin positive controls show an average inhibit zone of 23.31 mm. It was concluded that there was an effect of leaf extract and resin of chinese distance plant (Jatropha multifida L.) on growth of Staphylococcus aureus bacteria, and the best concentration was 100% concentration and almost closer to the inhibitory zone of Clindamycin antibiotics as positive control.

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