Assessment of prevalence of Fasciola hepatica and associated biochemical alterations in the cattle of Siirt province, Turkey

2018 ◽  
Author(s):  
Burçak Aslan Çelik ◽  
Özgür Yaþar Çelik ◽  
Kývanç Ýrak ◽  
Gülþah Akgül
Keyword(s):  
Biochemical Parameters ◽  
Zinc Sulfate ◽  
Fasciola Hepatica ◽  
Jugular Vein ◽  
Control Group ◽  
Elisa Method ◽  
Serum Samples ◽  
Fecal Examination ◽  
Biochemical Alterations ◽  
Flotation Method

The aim of this study is to determine the prevalence of Fasciola hepatica in the cattle raised in the Siirt province of Turkey and to obtain epidemiologic data about the disease in order to reveal its relationship with certain biochemical parameters. The material of the study consists of a total of 380 cattle. The blood sample was obtained from the jugular vein of the animals. Meanwhile, approximately 50-100 gr of feces were also collected from the animal’s rectum for a fecal examination. The zinc sulfate flotation method was used to inspect the fecal samples. 338 (89%) of samples were found to be seronegative, while the remaining 42 (11%) were seropositive. GGT, ALP, ALT, AST, TP and Albumin parameters analyzed on the serum samples. GGT levels of the seropositive animals were found to be statistically higher compared to the control group. As a result, the ELISA method was confirmed to be more accurate in the diagnosis of fasciolosis, and the increased GGT activity in the serum was determined as a significant indicator of the diagnosis.

scholarly journals Effects ofSalvadora persicaExtract on the Hematological and Biochemical Alterations against Immobilization-Induced Rats

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Kholoud S. Ramadan ◽  
Salha A. Alshamrani
Keyword(s):  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Hdl Cholesterol ◽  
Treated Group ◽  
Control Group ◽  
Serum Samples ◽  
Salvadora Persica ◽  
Biochemical Alterations ◽  
Hematological And Biochemical Parameters

A total of 24 rats were divided into 4 groups: control, stress, extract alone, and stress + extract (n=6each), for total 21 days of treatment. The immobilization stress was induced in rats by putting them in 20 cm × 7 cm plastic tubes for 2 h/day for 21 days. Rats were postorally treated withSalvadora persicaat a dose of 900 mg/kg body weight via intragastric intubations. At the end of the test period, hematological and biochemical parameters were determined in blood and serum samples with determination of vital organs weights. The vital organ weights were not significantly affected in stressed rats as compared to control rats. Compared to the control group, the stress treated group showed significances in several hematological parameters, including decreases in WBC, RBC, and PLT counts. Furthermore, in comparison to the control group, the stress group showed significantly increased blood glucose, serum total cholesterol, LDL-cholesterol, and triacylglycerols levels and decreased HDL-cholesterol level. The hematological and biochemical parameters in the stress + extract treated group were approximately similar to control group. The SP extract restored the changes observed following stress treatment.

scholarly journals Levels of certain biochemical and oxidative stress parameters in cattle with Brucellosis

2018 ◽  
Vol 68 (3) ◽  
pp. 285 ◽  
Author(s):  
K. BOZUKLUHAN ◽  
O. MERHAN ◽  
O. CELEBI ◽  
F. BUYUK ◽  
M. OGUN ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Biochemical Parameters ◽  
Jugular Vein ◽  
Reduced Glutathione ◽  
Control Group ◽  
Study Group ◽  
Serum Samples ◽  
Oxidative Stress Parameters ◽  
Healthy Cattle ◽  
And Oxidative Stress

The purpose of the present study was to determine concentrations of some biochemical parameters and oxidative stress levels in cattle with brucellosis. For this purpose, a study group included with 20 cattle with brucellosis and a control group with 10 clinically healthy cattle were used. Blood samples were collected into the tubes (with and without anticoagulant agent) from the Jugular vein of animals in each group. The reduced glutathione (GSH) in whole blood and levels of malondialdehyde (MDA) and nitric oxide (NO) were determined spectrophotometrically. Additionally, aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine and iron (Fe) levels in serum samples were colorimetrically determined. Compared with the animals in the control group, it was determined that cattle with brucellosis had significantly (P<0.01) higher levels of AST, ALT, creatinine and NO and lower level of Fe. The increases of MDA and GSH levels were moderate and significantly important (P<0.05) while serum urea manner was not significantly altered. It was determined that significant alterations occurred in various biochemical parameters and antioxidant activity decreased in cattle with brucellosis.

scholarly journals Adhesion-Regulating Molecule from Haemonchus contortus: Potential Antigen for Diagnosis of Early Infection in Goats

Pathogens ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 34 ◽  
Author(s):  
Kalibixiati Aimulajiang ◽  
Muhammad Ali-ul-Husnain Naqvi ◽  
Wen Chu ◽  
Mingmin Lu ◽  
Xiaowei Tian ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Fasciola Hepatica ◽  
Trichinella Spiralis ◽  
Clinical Signs ◽  
Serological Diagnosis ◽  
Economic Losses ◽  
Serum Samples ◽  
Fecal Examination ◽  
Diagnostic Potential ◽  
Blood Sucking

Haemonchus contortus, a blood-sucking nematode of ruminants, causes large economic losses worldwide. Diagnosis of infection mainly depends on the evaluation of clinical signs and fecal examination. However, this has limitations for the diagnosis of early or light infections, where serological diagnosis seems to be more accurate and reliable. In this study, the recombinant H. contortus adhesion-regulating molecule protein (rHCADRM) was expressed and purified, and its diagnostic potential was evaluated. Serum samples from goats experimentally infected with H. contortus (n = 5) were collected at 0 (before infection, negative control), 7, 14, 21, 35, 49, 63, 85, and 103 days post-infection (DPI). The reactions between rHcADRM and goat serum were tested using Western blot (WB) analysis. The results show that rHcADRM can be recognized in the serum as early as 14 DPI, and the antibody against rHcADRM in infected goat could be maintained for over 89 days. No reaction was found between rHcADRM and antibodies against Trichinella spiralis, Fasciola hepatica, or Toxoplasma gondii. An indirect enzyme-linked immune sorbent assay (ELISA) was developed based on rHcADRM. The optimal coating antigen (279 ng of rHcADRM/well) and serum dilutions (1:50) were determined by checkerboard titration. A total of 64 serum samples, including 32 from H. contortus infection goats and 32 from helminth-free goats, were used to determine the positive (0.362) and negative (0.306) cut-off values for the ELISA. The results show this serological diagnosis method is highly sensitive (90.6%) and specific (93.75%). The coefficient of variation within run and between runs was less than 11%. To apply this indirect ELISA during field examination, 51 serum samples were randomly collected from goat farms and tested using this method. The result showed that 19.6% (10/51) of goats were infected with H. contortus, which was 100% consistent with the necropsy result, higher than that of fecal examination (15.7%, 8/51). These results indicate that rHcADRM could be a potential antigen for diagnosis of H. contortus infection in goats.

​A Study on Alteration in Serum Biochemical Parameters in Colibacillosis Affected Diarrhoeic Cattle Calves

2021 ◽  
Author(s):  
T.C. Nayak ◽  
A.P. Singh ◽  
A. Chahar ◽  
Savita . ◽  
R. Yadav ◽  
...  
Keyword(s):  
Biochemical Parameters ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Control Group ◽  
Serum Biochemical Parameters ◽  
Serum Samples ◽  
Clinically Normal ◽  
Serum Biochemical ◽  
Result Analysis ◽  
G Ratio

Background: The objective of the present study was to evaluate the changes in serum biochemical parameters in colibacillosis affected diarrhoeic cattle calves below one month of age. Methods: The study was undertaken at Veterinary Clinical Complex, College of Veterinary and Animal Science, Rajasthan University of Veterinary and Animal Sciences, Bikaner, Rajasthan, India, during the period from September 2018 to March 2019. A total of 32 calves affected with colibacillosis confirmed on the basis of Multiplex PCR (for presence of K99, Stx1 and eaeA genes of E. coli) and eight clinically normal healthy calves (as control) were taken to investigate the serum biochemical profiles. Result: Analysis of serum samples of calves affected with colibacillosis revealed significant increase in the values of total protein, albumin, globulin, A/G ratio, blood urea nitogen, creatinine, potassium, total oxidant status andoxidative stress index while there is a significant decrease in the values of glucose, sodium, chloride, bicarbonate, IgG, IgM and total antioxidant status. There were no significant changes observed in the values of ALP, AST and ALT in affected calves as compared to healthy control group of calves.

scholarly journals Effect of Resveratrol on Hematological and Biochemical Alterations in Rats Exposed to Fluoride

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Nurgül Atmaca ◽  
Ebru Yıldırım ◽  
Bayram Güner ◽  
Ruhi Kabakçı ◽  
Fatih Sultan Bilmen
Keyword(s):  
Enzyme Activity ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
Treated Group ◽  
Inorganic Phosphorus ◽  
Control Group ◽  
Protective Effects ◽  
Biochemical Alterations ◽  
Fluoride Treatment ◽  
Hematological And Biochemical Parameters

We investigated the protective effects of resveratrol on hematological and biochemical changes induced by fluoride in rats. A total of 28 rats were divided into 4 groups: control, resveratrol, fluoride, and fluoride/resveratrol(n=7each), for a total of 21 days of treatment. Blood samples were taken and hematological and biochemical parameters were measured. Compared to the control group, the fluoride-treated group showed significant differences in several hematological parameters, including decreases in WBC, RBC, and PLT counts and neutrophil ratio. The group that received resveratrol alone showed a decrease in WBC count compared to the control group. Furthermore, in comparison to the control group, the fluoride group showed significantly increased ALT enzyme activity and decreased inorganic phosphorus level. The hematological and biochemical parameters in the fluoride + resveratrol treated group were similar to control group. In the fluoride + resveratrol group, resveratrol restored the changes observed following fluoride treatment, including decreased counts of WBC, RBC, and PLT, decreased neutrophil ratio and inorganic phosphorus levels, and elevated ALT enzyme activity. The present study showed that fluoride caused adverse effects in rats and that resveratrol reduced hematological and biochemical alterations produced by fluoride exposure.

scholarly journals Changes of Some Biochemical Parameters in Rats Supplemented with High Doses of Zinc Lactate

2016 ◽  
Vol 47 (4) ◽  
pp. 148-153
Author(s):  
V. Sloup ◽  
I. Jankovská ◽  
I. Langrová ◽  
M. Štolcová ◽  
S. Sloup ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Blood Serum ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Control Group ◽  
Feed Mixture ◽  
Serum Samples ◽  
Standard Mixture ◽  
Esterified Fatty Acids ◽  
High Doses

Abstract The experiment was conducted on 18 Wistar rats during a six-week period; 12 animals were given zinc lactate (120 mg/rat and week) in feed mixture and 6 control animals were fed a standard mixture for rats (ST-1). Sixteen biochemical parameters were measured from blood (serum) samples: total protein (TP), albumin (ALB), urea (UREA), glucose (GLU), triacylglycerols (TAG), non-esterified fatty acids (NEFA), cholesterol (CHOL), creatinine (CREAT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), uric acid (UA), magnesium (Mg), calcium (Ca), phosphorus (P), and trace elements such as Fe and Zn. When compared to the control group, we found that rats fed zinc lactate had higher concentrations of GLU, UA, UREA, Fe, Mg, Ca, TAG, TP, ALB, and ALP in the blood serum. Contrarily, the concentrations of AST, NEFA, CHOL, CREAT, P, and Zn were higher in the blood serum of control rats. Statistically significant differences between rats fed Zn and the control were found only in the concentrations of GLU, AST, ALP, UA, and P.

scholarly journals Evaluation of serum homocysteine and nitric oxide concentrations compared with other biochemical parameters in sheep naturally infected with Fasciola hepatica

2020 ◽  
Vol 71 (2) ◽  
pp. 2107
Author(s):  
V. DENIZHAN ◽  
S. KOZAT
Keyword(s):  
Nitric Oxide ◽  
Statistical Analysis ◽  
Vitamin B12 ◽  
Biochemical Parameters ◽  
Fasciola Hepatica ◽  
Control Group ◽  
Serum Homocysteine ◽  
Healthy Sheep ◽  
Animal Material ◽  
Folate And Vitamin B12

This study aims to determine the changes in serum homocysteine (Hcy) and nitric oxide (NO) concentrations in sheep naturally infected with F. hepatica. The animal material of the study consisted of a total of 50 sheep: 40 sheep with fascioliasis and 10 healthy sheep.The statistical analysis indicated that serum homocysteine concentrations, folate and vitamin B12 levels of the sheep infected with F. hepatica were higher than those of the control group (P<0.001 P<0.001 and P<0.05, respectively), whereas the nitric oxide levels of the sheep infected with F. hepatica were significantly lower than those of healthy sheep (P<0.001). In conclusion, it is thought that vitamin B12 and folate are not used sufficiently for the conversion of homocysteine to methionine in the remethylation cycle due to the damage in the liver tissue of sheep naturally infected with F. hepatica. This results in the increase of homocysteine which in turn inhibits the formation of nitric oxide.

scholarly journals Plasma Levels of IL-17, VEGF, and Adrenomedullin and S-Cone Dysfunction of the Retina in Children and Adolescents without Signs of Retinopathy and with Varied Duration of Diabetes

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Kornel Semeran ◽  
Przemysław Pawłowski ◽  
Łukasz Lisowski ◽  
Izabela Szczepaniak ◽  
Jerzy Wójtowicz ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Biochemical Parameters ◽  
Pediatric Patients ◽  
Control Group ◽  
Study Group ◽  
Elisa Method ◽  
Chronic Complications ◽  
Study Objective ◽  
Cone Dysfunction ◽  
Varied Duration

The study objective was to assess chosen biochemical parameters of blood and bioelectric function of the retina in patients with T1DM. The study group consisted of 41 patients with T1DM with no signs of diabetic retinopathy. The control group included 21 pediatric patients. We performed (1) S-cone ERG testing with retina response stimulation in both eyes at the luminance of 0.1, 0.2, and 0.5 (cd × s/m2) with the 440 nm blue flash and light application of the amber background (300 ph cd/m2, 495 nm wavelength), (2) anthropometric measurements, (3) biochemical investigations: IL-17, VEGF, and ADM by the ELISA method. A comparison of the ERG results with biochemical investigations indicates a likely correlation between the worsening of retinal bioelectric function and VEGF levels growing with diabetes duration. We showed a negative correlation between ADM and HbA1c and described possible causes of ADM reduction observed in subgroup I. We demonstrated the presence of bioelectric retinal dysfunction already before the diagnosis of diabetic retinopathy, which provides new possibilities in the diagnosis of preclinical chronic complications of diabetes. The changes observed in the levels of IL-17, ADM, and VEGF suggest their involvement in the diabetic pathogenesis of eye diseases.

Effects of Rosemary and husk on hemogram and some biochemical parameters during treatment of ulcerative colitis in rats

2019 ◽  
Vol 20 (1) ◽  
pp. 57-61
Author(s):  
Magdy Amer
Keyword(s):  
Ulcerative Colitis ◽  
Acetic Acid ◽  
Biochemical Parameters ◽  
Blood Platelets ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Albino Rats ◽  
Serum Samples

Objective: The effects of rosemary (10 mg/kg B.W) and husk (5 mg/kg B.W) on hemogram, GSH , CAT , NO, IL-1β and IL-10 of rats (40 albino rats) were investigated Design:Experimental study Animals: 40 albino rats Procedures: The 1st group was served as negative control, while the remaening groups (2nd, 3rd and 4th) were given acetic acid (4%) to induce ulcerative colitis (UC). Then the 2nd group was used as positive control, the 3rd group was orally given rosemary daily for 7 days, while the 4th group was orally administerated husk daily for 7 days. Blood and serum samples were collected from all groups at one week post dosing Results: The obtained results revealed that, both the tested plants induced a significant increase in RBCs, Hb, PCV% and lymphocyte counts and a significant decrease in WBC, neutrophil, monocyte and blood platelets of treated rats compared with the positive control group. Conclusion and clinical relevance: In addition rosemary and husk evoked a significant increase in GSH, CAT and IL-10 and a significant decrease in NO and IL-1β in serum of treated rats in comparions with the positive control group.

scholarly journals The Effect of Adding Cumin Cyminym Seeds to Concentrated Feed on Some Blood Biochemical Parameters of Black Local Goats

2020 ◽  
Vol 13 (1) ◽  
pp. 101-105
Author(s):  
Amal Awad ◽  
Mohamed Shwayel
Keyword(s):  
Total Protein ◽  
Biochemical Parameters ◽  
Jugular Vein ◽  
Control Group ◽  
College Of Agriculture ◽  
Blood Samples ◽  
Blood Biochemical Parameters ◽  
The Third ◽  
Blood Biochemical ◽  
Cumin Seed

The current study was conducted the effect of adding cumin cyminym seeds to concentrated feed on some blood biochemical parameters of black local goats. Fifteen female black goats aged 3 years, third milk production season. The study was in a private animal field of the Department of Animal Production- College of Agriculture/ University of Diyala, during the period from 1/11/2018 to 16/4/2019. The goats were divided randomly into three equal groups, 5 for each group. The first group (T1) left without treatment as a control group. The second group (T2) was given 10 g of cumin seed powder to the concentrated feed/ animal per day. The third group (T3) was given 20 g of cumin seed powder to the concentrated feed/ animal per day. Blood samples were collected before the experiment (Zero day) and at the end of the experiment (3 months), 10 ml from jugular vein by via vacationer tubes without anticoagulant. Blood biochemical parameters were also estimated includes: Total protein, Albumin, Globulin, Glucose, Urea and Triglycerides. The results showed no significant (P>0.05) in Total protein, Albumin, Globulin, Glucose, Urea and Triglycerides between (Zero day) and at the end of the experiment (3 months) and between different treated groups during the study period. It was concluded from this study that addition of cumin seed powder to the black local goats ration have no effects on some blood biochemical parameters.

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