Assessment of Adverse Effects of Lead, Nickel and Cadmium on Biochemical Parameters, Antioxidants Status and Metallothionein Expression in Buffaloes Slaughtered at Local Abattoir

2021 ◽  
Author(s):  
Himalaya Bhardwaj ◽  
Chanchal Singh ◽  
Shashi Nayyar
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Adverse Effects ◽  
Biochemical Parameters ◽  
Maximum Level ◽  
Lactic Dehydrogenase ◽  
Atomic Absorption Spectrophotometry ◽  
Health Concern ◽  
Control Group ◽  
Lead Nickel

Background: With the pace of industrialization, heavy metal level has been increasing in animals thereby causing deleterious effects, which emanate as public health concern, associated with their accumulation in food chain. The present study was undertaken to assess the systemic damage caused by heavy metals in buffaloes. Methods: The assessment of adverse effects of the lead (Pb), nickel (Ni) and cadmium (Cd), in blood was determined by monitoring the levels of biochemical parameters, antioxidants and expression of metallothionein in buffaloes. Blood and tissue (liver, kidney, pancreas and ovary) samples (n=50) were collected from local abattoir to estimate the levels of lead, nickel and cadmium using atomic absorption spectrophotometry. Antioxidants and biochemical parameters were estimated using standard procedures and while the expression of metallothionein-2 was analyzed using real-time PCR.Result: After determining the concentration of heavy metals in samples the buffaloes were classified as heavy metal exposed and non-exposed groups. The plasma level of heavy metals were found to be significantly (p less than 0.05) higher than the permissible limit in exposed buffaloes. In tissues, heavy metals levels were within the permissible limits. Malondialdehyde level in each of lead, nickel and cadmium exposed groups was significantly (p less than 0.05) higher than the non-exposed, control group. The antioxidant activity of superoxide dismutase, catalase and glutathione was found to be increased significantly (p less than 0.05) in exposed groups. Also, plasma glucose, cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH), creatine kinase (CK), urea and creatinine levels were found to be significantly higher (p less than 0.05) in the blood of the animal model used for the study. The fold change expression of MT-2 in blood was found to be at maximum level in cadmium and minimum in nickel exposed buffaloes. It can be concluded from the results of the experiment that heavy metals affect major organs (liver, kidney and muscle etc.) as evident by altered intracellular expression of MT-2, antioxidants and biochemical parameters.

Effect of Vitamin E and Selenium (Se) Supplementation on Biochemical Parameters and Expression of Metallothionein (MT-2) in Heavy Metals Exposed Buffaloes

2021 ◽  
Author(s):  
Himalaya Bhardwaj ◽  
Chanchal Singh ◽  
Shashi Nayyar ◽  
Sandeep Sodhi ◽  
Rajesh Jindal
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Vitamin E ◽  
Metal Binding ◽  
Biochemical Parameters ◽  
Lactic Dehydrogenase ◽  
Oral Feeding ◽  
Farm Animals ◽  
Blood Levels ◽  
Plasma Vitamin

Background: Farm animals may serve as bio-indicators of environmental pollution. Environmental heavy metals may disrupt the normal physiological and biochemical profile of the animals. The present study was planned to reduce the stress caused by heavy metal pollution by oral feeding of vitamin E and Selenium in heavy metals exposed buffaloes.Methods: Twenty buffaloes were selected on the basis of blood levels of heavy metals and divided into exposed and non-exposed groups. Exposed animals (n=10) were orally supplemented with 20 ml/day of Cargill E care Se® containing vitamin E, 100mg/ml and Se 0.5mg/ml for 30 days. Antioxidants, biochemical parameters and the expression of metallothionein-2 were analyzed after supplementation on 0, 15 and 30 days.Result: The levels of heavy metal were found to be elevated even after 30 days of supplementation. No significant alterations were observed in activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) after 30 days of supplementation. There was significantly (p less than 0.05) higher activity of blood glutathione and plasma Vitamin E. In supplemented group, plasma glucose, total cholesterol, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactic dehydrogenase (LDH), creatine kinase (CK) and total Immunoglobulin were found to be significantly reduced in supplemented group. Expression of metal binding protein, metallothionein-2 was found to be elevated in exposed animals despite supplementation with Vitamin E and Se for 30 days.

scholarly journals A New Ex Vivo Model to Evaluate the Hair Protective Effect of a Biomimetic Exopolysaccharide against Water Pollution

Cosmetics ◽  
2020 ◽  
Vol 7 (4) ◽  
pp. 78
Author(s):  
Claire Tubia ◽  
Alfonso Fernández-Botello ◽  
Jan Dupont ◽  
Eni Gómez ◽  
Jérôme Desroches ◽  
...  
Keyword(s):  
Water Pollution ◽  
Heavy Metals ◽  
Heavy Metal ◽  
Adverse Effects ◽  
Inductively Coupled Plasma ◽  
Ex Vivo ◽  
Polluted Water ◽  
Ex Vivo Model ◽  
Fiber Damage ◽  
The Impact

As an external appendage, hair is exposed to multiple stresses of different origins such as particles and gases in air, or heavy metals and chemicals in water. So far, little research has addressed the impact of water pollution on hair. The present study describes a new ex vivo model that allowed us to document the adverse effects of water pollutants on the structure of hair proteins, as well as the protective potential of active cosmetic ingredients derived from a biomimetic exopolysaccharide (EPS). The impact of water pollution was evaluated on hair from a Caucasian donor repeatedly immersed in heavy metal-containing water. Heavy metal retention in and on hair was then quantified using Inductively Coupled Plasma Spectrometry (ICP/MS). The adverse effects of heavy metals on the internal structure of hair and its prevention by the EPS were assessed through measurement of keratin birefringence. Notably, the method allows the monitoring of the organization of keratin fibers and therefore the initial change on it in order to modulate the global damage in the hair. Results revealed an increasing amount of lead, cadmium and copper, following multiple exposures to polluted water. In parallel, the structure of keratin was also altered with exposures. However, heavy metal-induced keratin fiber damage could be prevented in the presence of the tested EPS, avoiding more drastic hair problems, such as lack of shine, or decrease in strength, due to damage accumulation.

scholarly journals Heavy metal composition of maize and tomato grown on contaminated soils

2018 ◽  
Vol 3 (1) ◽  
pp. 414-426
Author(s):  
A.O. Adekiya ◽  
A.P. Oloruntoba ◽  
S.O. Ojeniyi ◽  
B.S. Ewulo
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Contaminated Soils ◽  
Heavy Metal Contamination ◽  
Mining Area ◽  
Atomic Absorption Spectrophotometry ◽  
Toxic Heavy Metals ◽  
Mining Site ◽  
Solanum Lycopersicum L ◽  
Sources Of Pollution

Abstract The study investigated the level of heavy metal contamination in plants {maize (Zea mays) and tomato (Solanum lycopersicum L.)} from thirty soil samples of three locations (Epe, Igun and Ijana) in the Ilesha gold mining area, Osun State, Nigeria. Total concentrations of As, Cd, Co, Cr, Cu, Ni, Pb and Zn were determined using atomic absorption spectrophotometry. Spatial variations were observed for all metals across the locations which was adduced to pH and the clay contents of the soils of each location. The results showed that heavy metals are more concentrated in the areas that are closer to the mining site and the concentrations in soil and plants (maize and tomato) decreased with increasing perpendicular distance from the mining site, indicating that the gold mine was the main sources of pollution. The mean concentrations of heavy metals in plants (tomato and maize) samples were considered to be contaminated as As, Cd and Pb respectively ranged from 0.6 - 2.04 mg kg-1, 0.8 - 5.2 mg kg-1, 0.8 - 3.04 mg kg-1 for tomato and respectively 0.60 - 2.00 mg kg-1, 1.50 - 4.60 mg kg-1 and 0.90 - 2.50 mg kg-1 for maize. These levels exceeded the maximum permissible limits set by FAO/WHO for vegetables. In conclusion, monitoring of crops for toxic heavy metals is essential for food safety in Nigeria.

scholarly journals Kandungan Logam Berat Besi (Fe) Pada Air, Sedimen, Dan Kerang Hijau (Perna viridis) Di Perairan Tanjung Emas Semarang

2015 ◽  
Vol 18 (1) ◽  
Author(s):  
Endang Supriyantini ◽  
Hadi Endrawati
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Flow Velocity ◽  
Atomic Absorption ◽  
Key Words ◽  
Atomic Absorption Spectrophotometry ◽  
Pollution Level ◽  
Perna Viridis ◽  
Absorption Spectrophotometry ◽  
Living Organisms

Logam berat Fe merupakan logam berat essensial yang keberadaannya dalam jumlah tertentu sangat dibutuhkan oleh organisme hidup, namun dalam jumlah yang berlebih dapat menimbulkan efek racun.Penelitian ini bertujuan untuk menganalisis kandungan dan tingkat pencemaran logam berat Fe pada air, sedimen, dan kerang hijau (Perna viridis) di perairan Tanjung Emas Semarang. Penelitian ini dilaksanakan pada tanggal 7 November dan 7 Desember 2013 dengan metode penelitian deskriptif. Logam berat Fe dalam sampel air, sedimen dan kerang hijau dianalisis di Laboratorium Balai Besar Teknologi Pencegahan Pencemaran Industri (BTPPI) Semarang dengan menggunakan metode AAS (AtomicAbsorption Spectrophotometry). Hasil penelitian menunjukkan bahwa perairan Tanjung Emas masih dalam taraf terkontaminasi logam Fe. Sedangkan pada sedimen dan pada kerang hijau (Perna viridis) sudah terindikasi tercemar logam Fe.Meskipun demikian variasi faktor lingkungan seperti suhu, salinitas, pH, kecepatan arus dan jenis sedimen juga memberikan kontribusi yang cukup penting terhadap kandungan logam Fe.Kata Kunci: logam Fe, Air, Sedimen, Perna viridis, metode AAS Heavy metalsiron(Fe) is anessentialheavy metalswhose presencein a certain amountis neededby living organisms, but inexcessiveamountscan causetoxic effects.The aims of the research is to analyze the heavy metals coccentration and the pollution level of Fe in water, sediment, and green mussels (Perna viridis) at Tanjung Emas Semarang. This research was conducted from 7 November and 7 December 2013 using the Atomic Absorption Spectrophotometry (AAS) and research methodswithdescriptive. The results showed that the waters of the Tanjung Emas is still in the stage of heavy metals contaminated iron (Fe). Sediment and green mussels (Perna viridis)already indicated heavy metal contaminatediron. However, variations inenvironmental factorssuch astemperature, salinity, pH, flow velocity an dsediment types also providean important contributionto heavy metal contentof iron(Fe).Key Words: Fe, water, sediment, Perna viridis, metode AAS

scholarly journals KANDUNGAN LOGAM BERAT (Cu, Pb, Zn, Cd, dan Cr) DALAM AIR DAN SEDIMEN DI PERAIRAN TELUK JAKARTA

2016 ◽  
Vol 11 (1) ◽  
pp. 9 ◽  
Author(s):  
Yani Permanawati ◽  
Rina Zuraida ◽  
Andrian Ibrahim
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Metal Content ◽  
Sea Water ◽  
Sediment Quality ◽  
Atomic Absorption Spectrophotometry ◽  
Quality Score ◽  
Subsurface Sediment ◽  
Absorption Spectrophotometry ◽  
Seawater Quality

Penelitian Lingkungan dan Kebencanaan Geologi Kelautan Perairan Teluk Jakarta (Tanjung Kait – Muara Gembong) dilakukan pada bulan Oktober - November 2010. Uji logam berat (Cu, Pb, Zn, Cd, dan Cr) dilakukan terhadap 28 contoh air laut dan 28 contoh sedimen dasar laut menggunakan metoda Atomic Absorption Spectrophotometry (AAS). Tujuan penelitian ini adalah untuk mendapatkan data dan informasi kondisi lingkungan terkini dari kandungan logam berat dalam air laut dan sedimen dasar laut. Kandungan logam berat (air laut dalam mg/l : sedimen dasar laut dalam ppm) terukur sebagai berikut : Cu ( <0.005 : 15.000-169.500 ); Pb (0.005-0.011 : 14.000-58.100); Zn (0.005-0.007 : 95.800-333.000); Cd (0.006-0.015 : 0.012-0.750); Cr (<0.001 : 45.320-139.180). Berdasarkan Metode Storet dapat dilihat nilai status mutu air laut perairan ini adalah -12, berarti bahwa kualitas air laut di perairan termasuk kelas C (tercemar sedang). Sedangkan status mutu sedimen di perairan menunjukkan skor nilai status mutu sedimen adalah 0, yang berarti bahwa kualitas sedimen termasuk kelas A (tidak tercemar/memenuhi baku mutu). Jelas terlihat bahwa nilai ambang batas (NAB) logam berat dalam sedimen jauh lebih tinggi dari NAB logam berat dalam air. Kata kunci: logam berat, sedimen dasar laut, air laut, nilai status mutu Marine Environmental and Geological Hazard Survey In Jakarta Bay Waters (Tanjung Kait - Muara Gembong) conducted in October-November 2010. Testing of heavy metals (Cu, Pb, Zn, Cd, and Cr) performed on 28 samples of sea water and 28 subsurface sediment samples using the method of Atomic Absorption Spectrophotometry (AAS). The purpose of this study was to obtain data and information on current environmental conditions of heavy metal content in seawater and subsurface sediment. Heavy metal content (sea water in mg/l: subsurface sediment in ppm) measured as follows: Cu ( <0.005 : 15.000-169.500 ); Pb (0.005-0.011 : 14.000-58.100); Zn (0.005-0.007 : 95.800-333.000); Cd (0.006-0.015 : 0.012-0.750); Cr (<0.001 : 45.320-139.180). Based on the Storet method shows the value of water quality status of sea waters is -12, which means that the seawater quality in these waters belong to a class C (medium contaminated). While the status of sediment quality in these waters indicate subsurface sediment quality score status value is 0, which means that subsurface sediment quality belongs to the class A (not polluted/correspond to the quality standards). It is clearly seen that the threshold value (NAV) of heavy metals in sediments is much higher than the NAV of heavy metals in water. Keywords: heavy metal, subsurface sediment, seawater, quality score status

scholarly journals Comparison of heavy metal accumulation capacity of some indigenous mosses in Southwest China cities: a case study in Chengdu city

2010 ◽  
Vol 56 (No. 2) ◽  
pp. 60-66 ◽  
Author(s):  
Y.E. Chen ◽  
S. Yuan ◽  
Y.Q. Su ◽  
L. Wang
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Inductively Coupled Plasma ◽  
Atomic Emission ◽  
Atomic Absorption Spectrophotometry ◽  
Heavy Metal Accumulation ◽  
Emission Spectrometry ◽  
Plasma Atomic Emission Spectrometry ◽  
Accumulation Capacity ◽  
The Difference

In order to evaluate the accumulation capacity of heavy metals in mosses, the total contents of eight elements (Cu, Zn, Fe, Mn, Ni, Pb, Cd, Cr) determined by Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) and Atomic Absorption Spectrophotometry (AAS) methods were compared in four types of indigenous mosses (<i>Brachythecium plumosum, Eurhynchium laxirete, Taxiphyllum taxirameum, Haplocladium strictulum</i>), which were collected from different sampling sites in the Chengdu city, China. The study found that heavy metal concentrations showed significant differences in interspecies and intraspecies comparison, while the accumulation capacity of <i>T. taxirameum</i> was higher than others. ANOVA analysis indicated that the atmospheric pollution of heavy metals in Wangjiang Park was relatively more serious than that of Ta Zishan Park and Cultural Park. The data also showed that the concentrations of heavy metals in the Chengdu city were higher compared to some foreign cities. The results are coincident with the previous conclusions that the difference of heavy metal depositions in mosses was not only related to environment, but also to their biological features.

scholarly journals Assessment of heavy metal’s in Atlantic sea fish sold in Benin

2020 ◽  
Vol 14 (5) ◽  
pp. 1853-1861
Author(s):  
Elisabeth Yehouenou Azehoun Pazou ◽  
Judicaël Azehoun Pazou ◽  
Mahoudjro Roméo Adamou
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Human Health ◽  
Fish Species ◽  
Health Impact ◽  
Atomic Absorption Spectrophotometry ◽  
Negative Effects ◽  
Fresh Fish ◽  
Sources Of Pollution ◽  
Metal Levels

Marine space is constantly threatened by various sources of pollution that may reduce its economic potential and have negative repercussions on human health. This study aims to assess heavy metals contamination of eight fish species and their impact on human health. Heavy metals such as cadmium, lead, copper and zinc were determined by atomic absorption spectrophotometry in fresh, cooked and fried fish. The results found in fresh fish (cadmium: 0.002 to 0.005 mg / kg, lead: 0 to 0.031 mg / kg, copper: 0.0045 to 0.305 mg / kg, zinc: 0.1245 to 0.6195 mg / kg), in cooked fish (cadmium: 0.0035 to 0.005 mg / kg, lead: 0 to 0.01 mg / kg, copper: 0 to 0.021 mg / kg, zinc: 0.171 to 0.327 mg / kg) and in fried fish (cadmium: 0.004 mg / kg, lead: 0 to 0.007 mg / kg, copper: 0 to 0.016 mg / kg, zinc: 0.0875 to 0.5065 mg / kg) showed that heavy metal levels are all below the standard values accepted by FAO / WHO. The consumption of these fish could therefore have no negative effect on the population’s health. Both types of cooking have both positive and negative effects on the heavy metal reduction in fish species. Although the concentrations of heavy metals are not worrying, the vigilance of the populations and a variation of the diet are important to guarantee a better health.Keywords: Heavy metals, health impact, Atlantic see fish, Cotonou.

scholarly journals THE IMPACT OF LEAD SALTS ON BIOCHEMICAL PARAMETERS IN THE LYMPH OF ANIMALS

REPORTS ◽  
2021 ◽  
Vol 335 (1) ◽  
pp. 83-89
Author(s):  
S.N. Abdreshov ◽  
B.М. Sеrik ◽  
А.Т. Mamatаeva ◽  
Р.S. Utegalieva ◽  
N.I. Zhаparkulоva
Keyword(s):  
Heavy Metal ◽  
Biochemical Parameters ◽  
Circulatory System ◽  
Scientific Basis ◽  
Acute Poisoning ◽  
Metal Salts ◽  
Control Group ◽  
Lead Salts ◽  
Heavy Metal Salts ◽  
The Impact

The article establishes the effect on the biochemical parameters of animal lymphocytes in case of poisoning with lead salts. The impact of lead salts, their spread, the effect of the harmful factors and the strengthening of the ecological situation in the environment determine the urgency of this problem. It is known that heavy metal ions gradually accumulate in the elements of the environment and then pass from the atmosphere to water and the human body. It was found that the effect of heavy metal salts in rats, including lead salts, has a negative effect on the lymphatic and circulatory system compared to the control group of biochemical parameters, which leads to metabolic disorders in the blood and lymph circulation. As a result of the study, the activity of rat enzymes was detected in normal and after poisoning with a toxicant. In acute poisoning, there was an increase in the amount of alkaline phosphatase in the lymphatic system by 40%, as well as an increase in the level of amino-transferase enzymes by 71% and 83%, respectively, and a decrease in the content of total bilirubin and associated bilirubin, all this indicates a violation of liver function, increased cytological processes in the liver and pancreas, decreased protein synthesis and impaired metabolism. When exposed to lead salts on the biochemical parameters of the lymph, the activity of α-amylase was reduced by 22.7%. based on the experiments, it was found that heavy metal salts significantly reduce the level of glucose in the blood, which negatively affects the parenchyma of the liver, kidney and kidney, increasing its consumption in tissues. The results obtained in the course of the study allowed us to form comprehensive data on the effect of lead on lymphatic and circulatory activity, biochemical parameters of the lymph of animal rats and can serve as a scientific basis for finding solutions to this problem.

scholarly journals Determination of Heavy Metals in Soil and Plants along Major Road in Hong Local Government Area, Adamawa State, Nigeria

2019 ◽  
pp. 1-10
Author(s):  
Nachana’a Timothy
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Metal Concentration ◽  
Heavy Metal Concentration ◽  
Soil Samples ◽  
Health Concern ◽  
Major Road ◽  
Roadside Soils ◽  
The Road

Heavy metal concentration in roadside soil and plants are increasingly becoming of health concern. This work determined the concentration of selected heavy metals (Cd, Pb, Zn, Cr, Fe, Mg, Mn, Co, Ni and Cu) in roadside soils and plants samples from selected sites (Plaifu, Shiwa, Fadama-rake and Damdrai) along major road in Hong. Soil samples were taken 10 m, 20 m and 30 m away from the edge of the road at the  depth of 0-10 cm, 10-20 cm and 20-30 cm. Plant samples were randomly collected within the vicinity where the soil samples were taken and were analysed using Atomic Absorption Spectrophotometer. The result revealed the trend in soil heavy metal concentration was Fe > Mn > Mg > Pb > Zn > Ni > Co > Cu > Cr > Cd and for plant the trend was Fe > Mn > Mg > Zn > Pb > Ni > Cu > Cd > Co > Cr. The concentrations decreased with increasing distance away from the edge of the road as well as with depth at which the soil sample were taken. The transfer factor showed that the concentration of Zn, Mn, Cu and Mg were greater than 1, which shows that plant were enriched by Zn, Mn, Cu and Mg from the soil. Mg and Cd equal to 1 at Plaifu and Damdrai. Most of the values of TF at the study area super pass 0.5, which implies that generally, the ability of bioaccumulation of these heavy metals in examined plants were relatively high.

scholarly journals Metallothionein expression on oysters (Crassostrea cuculata and Crassostrea glomerata) from the southern coastal region of East Java

F1000Research ◽  
2019 ◽  
Vol 8 ◽  
pp. 56 ◽  
Author(s):  
Asus Maizar Suryanto Hertika ◽  
Kusriani Kusriani ◽  
Erlinda Indrayani ◽  
Defri Yona ◽  
Renanda Baghaz Dzulhamdhani Surya Putra
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Laser Scanning ◽  
Coastal Region ◽  
Gill Tissue ◽  
Strong Relationship ◽  
Atomic Absorption Spectrophotometry ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Metal Levels

Background: This study aimed to analyse levels of heavy metals (Pb, Hg and Cd) in the aquatic body, gills and stomach of the oysters Crassostrea cuculata and Crassostrea glomerata, the metallothionein (MT) level in the gills and stomach of both oysters, and relationships between heavy metals level (Pb, Hg and Cd) in the gills and stomach to MT level in both types of oysters. Methods: The research method utilized was a descriptive method. The oyster samples were taken from three stations: Sendang Biru, Popoh and Prigi beaches. MT values were assessed using confocal laser scanning microscopy. The heavy metal levels were assessed using atomic absorption spectrophotometry method. Results: Both oyster heavy metal content obtained in the southern coastal waters exceeded the safe limit set by the State Minister of Environment No. 51 of 2004. In general, the expression of MT was found to be higher in gastric tissue compared to gill tissue. Conclusions: The relationship between levels of the heavy metals Pb, Hg, and Cd in oyster gills and stomach has a strong relationship with MT levels in the gills and stomach in both types of oysters.

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