Epidemiology, Haemato-biochemical and Pathological Changes Related to Field Outbreaks of PPR in Small Ruminants in Odisha

2021 ◽  
Author(s):  
P.K. Rath ◽  
S.K. Panda ◽  
B.P. Mishra ◽  
R. Mishra ◽  
D.K. Karna
Keyword(s):  
Small Ruminants ◽  
Pathological Changes ◽  
Chi Square ◽  
Tissue Samples ◽  
Helminthic Infection ◽  
Epidemiological Risk ◽  
Intracytoplasmic Inclusion Bodies ◽  
Polymerase Chain ◽  
Chi Square Analysis ◽  
Poor Management

Background: Odisha experiencing sporadic outbreaks of Peste des petits ruminants (PPR) throughout the year. There is a scarcity of available literature on PPR in Odisha till today. This is the first ever detail investigative approach in the state undertaken with an objective to corelate the epidemiological risk factors, haemato-biochemical and pathological changes in natural field outbreaks occurring in eight different districts. Methods: Fourteen field outbreaks of PPR were evaluated clinically as well as epidemiologically and confirmed through polymerase chain reaction (PCR). Blood, serum, faecal and tissue samples were collected to observe haemato-biochemical and pathomorphological changes to asses disease severity. Result: Present study concluded an overall mortality rate of 46.81%. Chi-square analysis revealed significant highest prevalence among 7-12 months (46.13%) age, Ganjam breed (45.51%) and females (80.49%). Frequent migration among the border areas along with poor management and helminthic infection was major precipitating factor. There was polycythemia along with neutrophilia and lymphopenia. Significant increase in alanine transaminase (ALT), aspartate aminotransferase (AST), K+ and Ca+2 along with creatinine, urea and blood urea nitrogen (BUN) BUN was observed in affected flocks. Antero-ventral consolidation of lungs, syncytia and presence of both eosinophilic intranuclear and intracytoplasmic inclusion bodies were major pathological changes.

scholarly journals Isolation ofMycobacterium aviumsubspeciesparatuberculosisfrom non-ruminant wildlife living in the sheds and on the pastures of Greek sheep and goats

2007 ◽  
Vol 136 (5) ◽  
pp. 644-652 ◽  
Author(s):  
M. FLOROU ◽  
L. LEONTIDES ◽  
P. KOSTOULAS ◽  
C. BILLINIS ◽  
M. SOFIA ◽  
...  
Keyword(s):  
Type Strain ◽  
Insertion Sequence ◽  
Small Ruminants ◽  
Dairy Sheep ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Sheep And Goats ◽  
Faecal Samples ◽  
Polymerase Chain ◽  
Type Strains

SUMMARYThis study aimed to: (1) investigate whether non-ruminant wildlife interfacing with dairy sheep and goats of four Greek flocks endemically infected withMycobacterium aviumsubspeciesparatuberculosis(MAP) harboured MAP and (2) genetically compare the strains isolated from the wildlife to those isolated from the small ruminants of these flocks. We cultured and screened, by polymerase chain reaction (PCR), pooled-tissue samples from 327 wild animals of 11 species for the MAP-specific IS900insertion sequence. We also cultured faecal samples from 100 sheep or goats from each of the four flocks. MAP was detected in samples from 11 sheep, 12 goats, two mice, two rats, a hare and a fox. Only one rat had histopathological findings. Genetic typing categorized 21 isolates as cattle-type strains and two, from a house mouse and a goat respectively, as sheep-type strains; this is the first report of a rodent harbouring a sheep-type strain. The MAP types that were most frequently isolated amongst the sheep and goats of each flock were also the ones isolated from sympatric rodents; those isolated from the fox and hare also belonged to the predominant ruminant strains.

Differences in Genetic Diversity for Mitochondrial DNA Between Hatchery and Wild Populations of Oncorhynchus

1994 ◽  
Vol 51 (S1) ◽  
pp. 290-297 ◽  
Author(s):  
Jennifer L. Nielsen ◽  
Christina Gan ◽  
W. Kelley Thomas
Keyword(s):  
Mitochondrial Dna ◽  
Chinook Salmon ◽  
Coho Salmon ◽  
Steelhead Trout ◽  
Wild Populations ◽  
Base Pairs ◽  
Chi Square ◽  
Variable Segment ◽  
Polymerase Chain ◽  
Chi Square Analysis

Amplification by the polymerase chain reaction and sequencing of a highly variable segment (199 base pairs) of mitochondrial DNA (mtDNA) in 491 fish from 22 streams and 9 hatcheries revealed 9 mtDNA types in steelhead trout (Oncorhynchus mykiss), 5 types in coho salmon (O. kisutch), and 6 types in chinook salmon (O. tshawytscha). Likelihood chi-square analysis of frequencies of mtDNA types in eight paired comparisons of hatchery and geographically proximate wild populations of Oncorhynchus showed significant differences in mtDNA genotypes. More mtDNA types were found, on average, in contemporary hatchery populations for each species than in geographically proximate wild stocks. Factors potentially leading to genetic differences in hatchery and wild stocks sampled in California were historic introductions of geographically divergent populations into hatchery brood stocks and lack of introgression of geographically divergent genotypes from the hatchery into wild populations.

scholarly journals Gambaran Epidemiologi Peningkatan Kasus Chikungunya di Desa Kajongan Kecamatan Bojongsari Kabupaten Purbalingga

2021 ◽  
pp. 127-136
Author(s):  
Nova Pramestuti ◽  
Ihda Zuyina Ratna Sari ◽  
Endang Setiyani ◽  
Ulfah Farida Trisnawati ◽  
Eva Lestari ◽  
...  
Keyword(s):  
Chikungunya Virus ◽  
P Value ◽  
Suspected Case ◽  
Chi Square ◽  
Specimen Collection ◽  
Polymerase Chain ◽  
Survey Interviews ◽  
Case Transmission ◽  
Chi Square Analysis ◽  
Case Data

Purbalingga is one of the chikungunya endemic districts which have cases increased until April in 2021 as many as 512 cases from the previous 116 cases in 2020. One of the areas of the increasing case occurred in Kajongan Village, Bojongsari Subdistrict. This study aims were to describe chikungunya cases, identify sources of transmission, and risk factors for increased chikungunya cases. A case-control design was performed in Kajongan Village in March 2021. The sample consisted of 36 cases and 36 controls. Cases are patients with suspected chikungunya, controls are people who are not chikungunya suspects who live close to the suspected case. Data was collected by blood specimen collection, larvae survey, interviews and examination using Reverse Transcriptase-Polymerase Chain Reaction. The results showed that the peak of case transmission occurred in the fourth week of February 2021. The main symptoms are fever and joint pain. Of the 21 blood samples examined, 19 (90.5%) tested positive for Chikungunya virus. Of the 9 pools Aedes sp. analyzed, 1 pool tested also positive Chikungunya virus. Chi-square analysis showed that the presence of larvae in container (p-value=0.03; OR=3.5; 95% CI=0.953-24.746) and mosquito nest eradication has not carried out routinely (p-value=0.04; OR=4.8; 95% CI=0.953-24.746) have been identified as a potential risk factor. The increasing of chikungunya cases in Kajongan Village was a local transmission supported by Chikungunya virus was detected in mosquitoes.

scholarly journals A Porcine Congenital Single-Sided Deafness Model, Its Population Statistics and Degenerative Changes

2021 ◽  
Vol 9 ◽  
Author(s):  
Wei Ren ◽  
Cong Xu ◽  
Fan-jun Zheng ◽  
Ting-ting Lin ◽  
Peng Jin ◽  
...  
Keyword(s):  
Hearing Loss ◽  
Hair Cell ◽  
Middle Ear ◽  
Pathological Changes ◽  
Chi Square ◽  
Population Statistics ◽  
Fifth Generation ◽  
Brainstem Response ◽  
Single Sided Deafness ◽  
Chi Square Analysis

ObjectiveTo describe and study the population statistics, hearing phenotype, and pathological changes of a porcine congenital single-sided deafness (CSSD) pedigree.MethodsClick auditory brainstem response (ABR), full-frequency ABR, and distortion product otoacoustic emission (DPOAE) were used to assess the hearing phenotype of the strain. Tympanogram was used to assess the middle ear function since birth. Celloidin embedding–hematoxylin–eosin (CE-HE) stain and scanning electron microscopy (SEM) were used to study the pathological changes of cochlear microstructures. Chi-square analysis was used to analyze the relation between hearing loss and other phenotypes.ResultsThe mating mood of CSSD with CSSD was most efficient in breeding-targeted CSSD phenotype (47.62%), and the prevalence of CSSD reached 46.67% till the fifth generation, where 42.22% were bilateral hearing loss (BHL) and 9.00% were normal hearing (NH) individuals. Hearing loss was proved to have no relation with coat color (P = 0.0841 > 0.05) and gender (P = 0.4621 > 0.05) by chi-square analysis. The deaf side of CSSD offspring in the fifth generation had no relation with that of their maternal parent (P = 0.2387 > 0.05). All individuals in this strain exhibited congenital severe to profound sensorineural hearing loss with no malformation and dysfunction of the middle ear. The good hearing ear of CSSD stayed stable over age. The deaf side of CSSD and BHL presented cochlear and saccular degeneration, and the hair cell exhibited malformation since birth and degenerated from the apex to base turn through time. The pathology in BHL cochlea progressed more rapidly than CSSD and till P30, the hair cell had been totally gone. The stria vascularis (SV) was normal since birth and degenerated through time and finally exhibited disorganization of three layers of cells.ConclusionThis inbred porcine strain exhibited high and stable prevalence of CSSD, which highly resembled human non-syndromic CSSD disease. This porcine model could be used to further explore the etiology of CSSD and serve as an ideal tool for the studies of the effects of single-sided hearing deprivation on neural, cognitive, and behavioral developments and the benefits brought by CI in CSSD individuals.

scholarly journals Fibrinonecrotic enteritis of piglets in a commercial farm: a postmortem study of the prevalence and the role of lesion associated agents Isospora suis and Clostridium perfringens

2007 ◽  
Vol 27 (7) ◽  
pp. 297-300 ◽  
Author(s):  
Macarena G. Sanz ◽  
Lucila Venturini ◽  
Ronnie A. Assis ◽  
Francisco Uzal ◽  
Miguel A. Risso ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Statistical Significance ◽  
Toxin Gene ◽  
Pathological Changes ◽  
Chi Square ◽  
The Third ◽  
Isospora Suis ◽  
Chi Square Analysis ◽  
Neutral Formalin

The objectives were to determine the prevalence of fibrinonecrotic enteritis (FNE) on a farrow-to-finish farm of 1,000 sows, to categorize the pathological changes, and to to investigate the lesion associated agents Isospora suis and Clostridium perfringens. Causes of preweaning mortality (PWM) were classified into 8 categories including FNE. Obtained data were evaluated for statistical significance by adjusted Chi-square analysis. Samples of FNE were taken for complementary studies including a PCR technique for genotyping toxin genes of Clostridium perfringens from gut samples fixed in 10% neutral formalin. From 3,153 piglets examined, less than 1% was classified as FNE. FNE prevalence increased progressively from the first to the third week, the last differing statistically from the others. Eighty percent of gut samples with FNE lesions were positive to Isospora suis, when examined by PCR from 9 severe FNE lesions detected 7 positive samples only for a toxin gene, characteristic of C. perfringens type-A.

PERSEPSI PETERNAK PADA KEPEMIMPINAN PENDAMPING DAN HUBUNGANNYA DENGAN PRODUKTIVITAS USAHA TERNAK DI PAGUYUBAN AL-AWWALIYAH, PASIRWANGI, GARUT, JAWA BARAT

2019 ◽  
Vol 12 (1) ◽  
pp. 42-51
Author(s):  
Dwi Indah Sulistiani ◽  
Ujang Maman ◽  
Junaidi J
Keyword(s):  
Data Analysis ◽  
Secondary Data ◽  
Primary Data ◽  
Leadership Qualities ◽  
Chi Square ◽  
Education Experience ◽  
Spss Software ◽  
Chi Square Analysis ◽  
And Behavior ◽  
The Relationship

Objective of this research; 1) determine the perception of ranchers against the properties and behavior of the leadership of the companion in the Society of Al-Awwaliyah 2) analyze the relationship between productivity breeder with productivity of livestock in the Society of Al-Awwaliyah 3) identify the relationship perceptions of ranchers against the leadership companion with productivity of livestock in the Society of Al-Awwaliyah , The data used in this study are primary and secondary data. Primary data were obtained from questionnaires which stem from ranchers while secondary data sourced from literature in the form of books and articles. Data processing was performed using Chi-square analysis using SPSS software version 21. One of the factors relating to the productivity of ranchers is the perception of ranchers against the leadership of their companion. Leadership companion views of the nature and behavior of which is owned by a companion. Productivity ranchers indirectly related to the productivity of the cattle business. Characteristics breeder visits of age, years of education, experience ranchers, and businesses in addition to ranchers. The results of data analysis showed that there is a significant relationship between business other than ranchers with ranchers productivity. The relationship between the perception of the nature of the companion breeder with productivity ranchers produce Pearson Chi-Square value is 9.751 and Asymp. Sig. (2-sided) of 0.002. This is due to interest ranchers against leadership qualities possessed by a companion who produce prolific ranchers. Ranchers consider that a companion of his leadership qualities are ideal as a companion.

The Effect of Sanctions on Cooperative Behavior: A Study on Medium Trust Individuals in the Context of Corruption

2018 ◽  
Vol 34 (1) ◽  
Author(s):  
Dewi Rosiana ◽  
Achmad Djunaidi ◽  
Indun Lestari Setyono ◽  
Wilis Srisayekti
Keyword(s):  
Logistic Regression ◽  
Public Interest ◽  
Logistic Regression Model ◽  
Cooperative Behavior ◽  
Research Subjects ◽  
Chi Square ◽  
Public Interests ◽  
The Public ◽  
Chi Square Analysis ◽  
Personal Interests

This study aims to describe the effect of sanctions (individual sanctions, collective sanctions, and absence of sanctions) on cooperative behavior of individuals with medium trust in the context of corruption. Both collective sanctions and individual sanctions, are systemic, which means sanctioning behavior is exercised not by each individual but by the system. Cooperative behavior in this context means choosing to obey rules, to reject acts of corruption and to prioritize public interests rather than the personal interests. Conversely, corruption is an uncooperative behavior to the rules, and ignores the public interest and prioritizes personal interests. Research subjects were 62 students. The Chi-Square Analysis was used to see the association between the variables and the logistic regression model was applied to describe the structure of this association. Individual sanction is recommended as punishment to medium trust individuals to promote cooperative behavior in the context of corruption. The results showed that individuals with medium trust had more cooperative behavior.

Evaluation of a New Clinical Test of Fusion Status: A Pilot Study

2019 ◽  
pp. 113-118
Keyword(s):  
Pilot Study ◽  
Ohio State University ◽  
Background Suppression ◽  
Clinical Test ◽  
State University ◽  
Chi Square ◽  
Central Fusion ◽  
Eye Examination ◽  
The Ohio State University ◽  
Chi Square Analysis

Background Suppression is associated with binocular vision conditions such as amblyopia and strabismus. Commercial methods of testing fusion often only measure central fusion or suppression at near. The purpose of this pilot study was to assess a new iPad picture fusion test that assesses foveal and central fusion at near. Methods Participants aged 5 years and older presenting for eye examination at The Ohio State University College of Optometry were enrolled. Results from visual acuity, dry and wet refraction/retinoscopy, stereopsis and cover testing were recorded from the patient chart. The iPad picture fusion test, Worth four-dot, Worth type test with foveal letter targets, and Polarized four-dot were performed by one examiner in a randomized order at 40 cm. Testing was repeated with the anaglyphic filters reversed. Crosstabulation and McNemar chi-square analysis were used to compare the results between fusion testing devices. Results Of the fifty participants (mean age = 17.5), twelve reported suppression and one reported diplopia. Testability was excellent for all tests (98% to 100%). There were no significant differences between tests in reported results (P ≥ 0.22 for all comparisons). No difference in reported fusion or suppression status was observed with change in orientation of the anaglyphic filters. Six participants reported foveal suppression alone at near which was not identified with Worth four-dot at near. Conclusion The iPad picture fusion test provided excellent testability and agreement with commonly used tests of fusion and allowed testing of both central and foveal fusion at near. Nearly half (46%) of participants with suppression reported foveal suppression, supporting the importance of testing for foveal suppression.

PENGARUH PEMBERIAN PELAYANAN INFORMASI OBAT DAN KONSELING TERHADAP TINGKAT KEPATUHAN MINUM OBAT PADA PASIEN DIABETES MELITUS DENGAN HIPERTENSI DI RUMAH SAKIT DAERAH IDAMAN BANJARBARU

2020 ◽  
Vol 5 (2) ◽  
pp. 240-249
Author(s):  
Satrio Wibowo Rahmatullah ◽  
◽  
Ika Maulida Nurrahma ◽  
Adnan Syahrizal
Keyword(s):  
Drug Information ◽  
Treatment Success ◽  
Information Service ◽  
Regional Hospital ◽  
P Value ◽  
Chi Square ◽  
Cross Sectional ◽  
Quasi Experimental ◽  
High Level ◽  
Chi Square Analysis

Compliance to achieve treatment success can be improved by providing drug information services (PIO) and counseling to improve understanding of treatment instructions. The general objective of this study was to determine the effect of drug information service and counseling on the level of medication adherence in patients with diabetes mellitus (DM) with hypertension in the Banjarbaru Regional Hospital. This study uses a quasi experimental method with a cross sectional approach and takes patient data prospectively. The results of this study indicate that the group given PIO and counseling showed a high level of adherence as many as 20 respondents (100%). Whereas in the group that was not given PIO and the counseling level of adherence was low, there were 4 respondents (20%). Based on the mann-whitney analysis p-value = 0,000 (<α = 0.05) so that there are differences in the group with PIO and counseling with groups without PIO and counseling, while the chi square analysis p-value = 0.004 (<α = 0 , 05) so that PIO and counseling have a significant effect on the level of adherence to taking medication in patients with DM with hypertension at the Banjarbaru Regional Hospital.

Trends in the incidence and mortality of oral cancer in Nevada (Preprint)

2020 ◽  
Author(s):  
Kevin Foote ◽  
Karl Kingsley
Keyword(s):  
Public Health ◽  
Oral Cancer ◽  
Cancer Incidence ◽  
Cancer Control ◽  
Cancer Registries ◽  
Public Health Research ◽  
Chi Square ◽  
Incidence And Mortality ◽  
The Us ◽  
Chi Square Analysis

BACKGROUND Reviews of national and state-specific cancer registries have revealed differences in rates of oral cancer incidence and mortality that have implications for public health research and policy. Many significant associations between head and neck (oral) cancers and major risk factors, such as cigarette usage, may be influenced by public health policy such as smoking restrictions and bans – including the Nevada Clean Indoor Act of 2006 (and subsequent modification in 2011). OBJECTIVE Although evaluation of general and regional advances in public policy have been previously evaluated, no recent studies have focused specifically on the changes to the epidemiology of oral cancer incidence and mortality in Nevada. METHODS Cancer incidence and mortality rate data were obtained from the National Cancer Institute (NCI) Division of Cancer Control and Population Sciences (DCCPS) Surveillance, Epidemiology and End Results (SEER) program. Most recently available rate changes in cancer incidence and mortality for Nevada included the years 2012 – 2016 and are age-adjusted to the year 2000 standard US population. Comparisons of any differences between Nevada and the overall US population were evaluated using Chi square analysis. RESULTS This analysis revealed that the overall rates of incidence and mortality from oral cancer in Nevada differs from that observed in the overall US population. For example, although the incidence of oral cancer among Caucasians is increasing in Nevada and the US overall, it is increasing at nearly twice that rate in Nevada, P=0.0002. In addition, although oral cancer incidence among Minorities in the US is declining, it is increasing in Nevada , P=0.0001. Analysis of reported mortality causes revealed that mortality from oral cancer increased in the US overall but declined in Nevada during the same period (2012-2016). More specifically, mortality among both Males and Females in the US is increasing, but is declining in Nevada, P=0.0027. CONCLUSIONS Analysis of the epidemiologic data from Nevada compared with the overall US revealed significant differences in rates of oral cancer incidence and mortality. More specifically, oral cancer incidence increased in Nevada between 2012-2016 among all groups analyzed (Males, Females, White, Minority), while decreases were observed nationally among Females and Minorities. Although mortality in Nevada decreased over this same time period (in contrast to the national trends), the lag time between diagnosis (incidence) and mortality suggests that these trends will change in the near future. CLINICALTRIAL Not applicable

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