scholarly journals Degradation of Petroleum Sludge in Soil by Bacterial-Fungal Co-Culture in Presence of Organic and Inorganic Stimulants

2019 ◽  
Vol 5 (03) ◽  
pp. 155-164
Author(s):  
Babita Kumari ◽  
Manvi Singh ◽  
Pankaj Kumar Srivastava ◽  
S. N. Singh
Keyword(s):  
Microbial Consortium ◽  
Abiotic Factors ◽  
Organic Fertilizer ◽  
Inorganic Fertilizer ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Catabolic Enzymes ◽  
Petroleum Sludge ◽  
Induction Of Enzymes ◽  
Curvularia Verruculosa

A microcosmic study was carried out for degradation of petroleum sludge [4% (w/w) in soil] in presence of constructed microbial consortium of three bacterial strains i.e., Pseudomonas sp. BP10, Acinetobacter sp. PSM11 and Rhodococcus sp. NJ2 and two fungal strains Panicillium oxalicum PS10 and Curvularia verruculosa PS8, isolated from different petroleum hydrocarbon contaminated sites) supplemented with vermicompost and inorganic fertilizer as biostimulants. After six months of incubation, the maximum degradation of TPH from petroleum sludge was recorded as high as 80% in the presence of combination of inorganic and organic fertilizer and microbial consortium while only 33% degradation was attributed by native organisms and abiotic factors. Enhancement (%) in degradation rate of TPH due to addition of vermicompost, inorganic fertilizer and microbial consortium in separation and combination was recorded as 57%, 13%, 35% and 139%, respectively. Besides the enhancement in specific growth rate of soil microbes due to addition of nutrient, bioaugmentation of this constructed microbial consortium also boost the total bacterial and fungal strains present in petroleum sludge contaminated soil. Catabolic enzymes played an important role in degradation and maximum induction of enzymes likes catechol 1, 2 dioxygenase, catechol 2, 3 dioxygenase, catalase, laccase and dehydrogenase activity were recorded 223.89 μ mol g-1, 323.83 μ mol g-1, 0.714 μ mol H2O2 g-1, 0.623 μ mol g-1 and 3.4 μg g-1 h-1, respectively.

PEMANFAATAN LIMBAH JAGUNG MENJADI PUPUK ORGANIK UNTUK PENYUBURAN LAHAN PERTANIAN

2018 ◽  
Vol 6 (1) ◽  
Author(s):  
Daru Mulyono
Keyword(s):  
Agricultural Land ◽  
Organic Fertilizer ◽  
Economic Value ◽  
Soil Microorganism ◽  
Appropriate Technology ◽  
Inorganic Fertilizer ◽  
Plant Materials ◽  
Chemical Soil ◽  
Stem Leaf

The use of maize waste plant materials (stem, leaf, and husk cover) have high economic value to be processed become organic fertilizer for agricultural land fertilizer. Maize have several and quite high contents of macro and micro nutrients. This activity was hoped that the farmers can overcome the increasing price of inorganic fertilizer recently and furthermore farmers can reap higher income. Beside higher income the use of organic fertilizer can improve the nature and behaviourof land through improving of soil chemical, soil physical, and soil microorganism. Therefore, the appropriate technology for processing of maize become organic fertilizer is very important to be diffused or socialized to farmers.Keywords: fertilizer, maize waste

scholarly journals PENGARUH PUPUK ZA DAN KOMPOS TERHADAP KANDUNGAN Pb, Zn, Cu DAN N TANAH SERTA HASIL TANAMAN PADA SISTEM BUDIDAYA BAWANG MERAH DI TEPI DANAU BATUR, KINTAMANI, BANGLI

2020 ◽  
Vol 14 (2) ◽  
pp. 120
Author(s):  
Shinta Lestari Santosa ◽  
I Nyoman Rai ◽  
Wayan Diara
Keyword(s):  
Crop Production ◽  
Crop Yields ◽  
Organic Fertilizer ◽  
Nutrient Content ◽  
Chemical Fertilizer ◽  
Inorganic Fertilizer ◽  
Growth And Yield ◽  
Cu Content ◽  
Soil Zn ◽  
Vegetable Cultivation

Vegetable cultivation is livelihoods for side Lake Batur communities, Kintamani, Bangli. Hilly natural conditions with a soil texture influenced by the eruption of Mount Batur, 900 m above sea level, and 900-3500 mm high rainfall, causing this region is very suitable for the cultivation of various vegetables, including shallot. One effort to meet the high demand for shallots is that efforts are made to improve cultivation techniques, including fertilizing to improve yields. In modern agriculture, the use of fertilizer is absolutely essential to trigger the level of crop production. The aims is to analyze the combination effect of using of inorganic fertilizer (ZA fertilizer) and organic fertilizer (compost fertilizer) on shallot vegetable cultivation systems on the content of pollutants, N nutrients and onion crop yields on the shores of Lake Batur, Kintamani District, Bangli Regency. The study using RBD with two factors where factor I: provision of organic fertilizer is leaf compost made aerobically (O), consisted of 3 levels, namely: O0 = 0*, O1 = 5* and O2 = 10*and factor II: the application of inorganic fertilizer namely ZA (S) fertilizer, consisted of 3 levels, namely: S0 = 0**, S1 = 50** and S2  = 100**, each repeated 3 times. The parameters observed were the growth and yield of shallots as well as the content of Pb, Zn, Cu and N nutrients in the soil. The nutrient content (N) in the soil, when using chemical fertilizer ZA and compost organic is not significantly different, as well as the results of onion plants, while the content of Pb, Zn and Cu on the use of chemical fertilizer ZA and organic compost, very real different. The highest soil Pb content in S2O1 treatment is 30.07***, the highest soil Zn content in the S2O1 treatment was 28.24***, and the highest soil Cu content in the S1O2 treatment is 17.22***. *= tons/ha **= kg/ha ***= mg/kg Keywords: compost; contents Pb; Zn; Cu of soil; shallot; ZA.

scholarly journals Peran Pupuk Organik dalam Peningkatan Efisiensi Pupuk Anorganik pada Padi Sawah (Oryza sativa L.)

2015 ◽  
Vol 43 (1) ◽  
pp. 8
Author(s):  
Totong Siwanto ◽  
Sugiyanta , ◽  
Maya Melati
Keyword(s):  
Oryza Sativa L ◽  
Block Design ◽  
Organic Fertilizer ◽  
Biological Properties ◽  
Paddy Rice ◽  
Inorganic Fertilizer ◽  
Growth And Yield ◽  
Research Station ◽  
Randomized Block Design ◽  
Two Factors

ABSTRACT<br />Application of organic fertilizer into the soil is designated as a source of macro, micro nutrients and organic acids; it also acts as soil ameliorant that improve physical, chemical and biological properties of soil. Application of high rates organic fertilizer has a constraint namely the availability and ease of application. Therefore, it is necessary to learn the use of organic fertilizer with low rates to improve the efficiency of chemical fertilizers. This research was aimed to study the effect of organic and inorganic fertilizers on growth and yield of paddy rice, as well as the efficiency of N, P, K inorganic fertilizer. Field experiment was conducted at Sawah Baru Research Station, University Farm IPB, Bogor, West Java from September to December 2013. The experiment used Randomized Block Design consisted of two factors: rates of organic fertilizer (0, 250, 500, 750, and 1,000 kg ha-1), and rates of inorganic fertilizer (0, 100, 200, 300, and 400 kg ha-1). The results suggested that the application of 1,000 kg organic fertilizer ha-1 + 400 kg inorganic fertilizer ha-1 did not show significant interaction. Application of organic fertilizer alone up to 1,000 kg ha-1 resulted in low growth and yield. Increasing rates of  application up to 400 kg inorganic fertilizer ha-1 increased the growth and yield of paddy rice. The highest N efficiency was 89.19% at a rate of 500 kg organic fertilizer ha-1 + 200 kg inorganic fertilizer ha-1, whereas the highest efficiency of P and K, respectively were 69.55% and 92.52% at a rate of 750 kg organic fertilizer ha-1 + 300 kg inorganic fertilizer ha-1.<br />Keywords: NPK, organic fertilizer, paddy rice

scholarly journals Biosorption potential and molecular characterization of metal resistant autochthonous microbes from tannery solid waste

2021 ◽  
Author(s):  
Hajira Younas ◽  
Aisha Nazir ◽  
Zakia Latif ◽  
Janice E Thies ◽  
Muhammad Shafiq ◽  
...  
Keyword(s):  
Solid Waste ◽  
Its Region ◽  
Metal Resistance ◽  
Molecular Techniques ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Resistant Strains ◽  
Tannery Solid Waste ◽  
Clean Metal

This study encompasses isolation and screening of heavy metal-resistant fungal and bacterial strains from tannery solid waste (TSW). Twelve fungal strains and twenty-five bacterial strains were isolated from TSW. The growth of fungal strains was observed against different heavy metals ranging from 10 mg L -1 to 1050 mg L -1 and the growth of bacteria was observed in metal concentrations ranging from 10 mg L -1 to 1200 mg L -1 . Five multi-metal resistant fungal isolates belonging to the genus Trichoderma and ten bacterial isolates belonging to the genus Bacillus showed good metal resistance and biosorption potential. They were identified through molecular techniques, fungi based on ITS region ribotyping, and bacteria based on 16S rRNA ribotyping. The fungal strains were characterized as T. hamatum (TSWF-06), T. harzianum (TSWF-11), T. lixii (TSWF-02) and T. pseudokoningii (TSWF-03, TSWF-10). The bacterial strains were characterized as Bacillus xiamenensis (TSW-02), B. velezensis (TSW-05), B. piscis (TSW-06), B. safensis (TSW-10), B. subtilis (TSW-14, TSW-15, TSW-17) B. licheniformis (TSW-19), B. cereus (TSW-20) and B. thuringiensis (TSW-22). The fungal strains namely, T. pseudokoningii (TSWF-03) and T. harzianum proved to be two multi-metal resistant strains with good biosorption efficiency. Unlike fungi, bacterial strains showed metal specific resistance. The strains Bacillus xiamenensis , B. subtilis (TSW-14) and B. subtilis (TSW-15) showed good biosorption efficiency against Cr, B. safensis against Cu, B. piscis and B. subtilis (TSW-17) against Pb and B. licheniformis and B. thuringiensis against Zn. The autochthonous fungal and bacterial strains can therefore be employed to clean metal contaminated environments.

scholarly journals Respon Pertumbuhan Tanaman Cengkeh (Syzygium aromaticum (L.) Merr. Perr.) dengan Aplikasi Pupuk Organik-Anorganik dan Triakontanol

2020 ◽  
Vol 47 (3) ◽  
pp. 326-332
Author(s):  
Syahdin Launuru ◽  
Ade Wachjar ◽  
Dan Ani Kurniawati
Keyword(s):  
Leaf Area ◽  
Nutrient Dynamics ◽  
Block Design ◽  
Organic Fertilizer ◽  
Inorganic Fertilizer ◽  
Syzygium Aromaticum ◽  
Chlorophyll B ◽  
Recommended Dosage ◽  
Randomized Complete Block Design ◽  
Two Factors

Application of right dosage of fertilizer and concentration of plant growth regulator are supporting factor of clove growth. This study was aimed to obtain the best combination of organic-inorganic fertilizer, the best triacontanol concentration and both interactions for increasing clove growth. This study used a Randomized Complete Block Design (RCBD) with two factors. The first factor was the organic-inorganic fertilizer dosage package that consisted of 6 levels, i.e no fertilizer, 100% recommended dosage of inorganic fertilizer (r.d.i.f), 25% recommended dosage of organic fertilizer (r.d.o.f) + 75% r.d.i.f, 50% r.d.o.f + 50% r.d.i.f, 75% r.d.o.f + 25% r.d.i.f, 100% r.d.o.f. The second factor was the concentration of triacontanol that consisted of 3 levels, i.e no triacontanol, 2 mg L-1 and 4 mg L-1. The results showed that the application of 25% r.d.o.f + 75% r.d.i.f produced the best clove growth, but it was not significantly different from the combination treatment of the two other types of fertilizer as indicated by the greatest improvement on stem diameter, branches number, leaf area, chlorophyll a, chlorophyll b, total chlorophyll and carotenoids. Clove plants did not respond to the application of triacontanol at a concentration of 2 mg L-1, but gave a negative response to the application of triacontanol 4 mg L-1 on the plant height and leaf area variables. The interaction of the two factors did not have significant effects on all variables. Keywords: chlorophyll, morphology, nutrient dynamics, physiology, vegetative growth  

scholarly journals ANTIFUNGAL AND ANTIBACTERIAL ACTIVITY OF ALOE VERA PLANT EXTRACT

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
P Danish ◽  
Q Ali ◽  
MM Hafeez ◽  
A Malik
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Aeruginosa ◽  
Aspergillus Niger ◽  
Bacillus Megaterium ◽  
Aloe Vera ◽  
Water Soluble ◽  
Root Extract ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
Fungal Strains

Aloe vera is a well-known medicinal plant used in many therapeutic purposes. Naturally it is composed of many useful compounds that have ability to use for treatment of many diseases. The active compounds reported in this plant are saponins, sugar, enzymes, vitamins, aloesin, aloeemodin, aloin, acemannan aloemannan, aloeride, methylchromones, flavonoids, naftoquinones, sterols, minerals, anthraquinones, amino acids, lignin and salicylic acid and other different compounds including fat-soluble and water-soluble vitamins, enzymes, minerals, simple/complex sugars, organic acid and phenolic compounds. In this study aloe vera is used for antibacterial and antifulgal activity against different strains of bacteria and pathogenic fungal strains. Ethanol extract of Aloe vera leaves and roots is applied on these bacterial and fungal strains in different concentrations (15, 20, 25, 30µl). Bacillus cereus, Bacillus subtitis, Bacillus megaterium, Streptococcus pyogenes, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Acinetobacter baumannii, and some other bacterial strains are used for this study. Escherichia coli and Agrobacterium tumefacins shows zone of inhibition around 18mm which consider as good result. Bacillus subtitis and Bacillus megaterium also shows good result around 16mm. Proteus mirabilis and Pseudomonas aeruginosa shows minimum zone of inhibition which is around 11mm. among all used fungal strains (fuserium oxysporum, Candida albicans, Aspergillus fumigatus, Aspergillus niger) fuserium oxysporum and Aspergillus niger shows excellent results around 19mm both against root extract and leaves extract.

scholarly journals Peningkatan Kapasitas Petani Melalui Pelatihan Pembuatan Pupuk Organik Bokashi di Daerah Perbatasan Darat Kalimantan Barat

DEDIKASI ◽  
2019 ◽  
Vol 21 (1) ◽  
Author(s):  
Arief Sirajuddin ◽  
Kartika Ekasari ◽  
Racmat Racmat ◽  
Aminuddin Saade
Keyword(s):  
Rice Straw ◽  
Organic Fertilizer ◽  
Inorganic Fertilizer ◽  
Knowledge And Skills ◽  
The People ◽  
The Village

This activity was motivated by the fact that the people in Mentibar Village, Paloh Sub-district of SambasRegency are farmers who had only relied on their farming activities using of inorganic fertilizer especially Ureafertilizer from Malaysia, which the price is not cheap and sometimes the unavailable. The farmers in Mentibarvillage are new to Bokashi organic fertilizer, which its main ingredients are widely available in the village such aslivestock, fine bran, straw, etc. In this study, we conducted training and counselling to improve farmers' knowledgeand skills in making organic fertilizer. Bokashi as an alternative fertilizer can be used by farmers in their ricecultivation and at the same time to less reliant to inorganic fertilizer. The purpose of this Community Serviceactivities are: First, to increase the knowledge and skills of farmers in making Bokashi organic fertilizer. Second, toprovide an understanding that relying on inorganic fertilizer can damage the soil and produce pollution. Third,farmers will be able to utilize the materials around them to be used as Bokashi fertilizers such as livestock, bran,husks and rice straw.

scholarly journals Isolation and characterization of fungal and bacterial proteolytic strains from chrome shavings

2020 ◽  
Author(s):  
Mariana Ferdes ◽  
Rodica Roxana Constantinescu
Keyword(s):  
Culture Media ◽  
Proteolytic Enzymes ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Isolation And Characterization ◽  
Finishing Process ◽  
The Media ◽  
Specific Culture ◽  
Yeasts And Molds ◽  
Leather Finishing

The chrome shavings waste obtained as a result of the leather finishing process accumulates in a large volume in tanneries and represent a major problem for the environment. This waste are particularly resistant to attack of microorganisms, due to the significant concentration of chromium and are thus difficult to degrade. In this study, chrome shavings were analyzed microbiologically by determining the total number of germs and the number of yeasts and molds on specific culture media. Several bacterial and fungal strains were isolated from the cultures in Petri dishes, after the growth of the colonies. These strains were characterized in terms of the production of proteolytic enzymes, by a method of screening on the media with casein, which allows the determination of proteolytic indices of microorganisms. As a result of the tests performed, five bacterial strains probably belonging to the genus Bacillus and two fungal strains from the genera Penicillium and Cladosporium were selected.

Phytochemical profile, antimicrobial, antioxidant and antiobesity activities of Scolymus angiospermus Gaertn. Four fractions from Jericho/Palestine

2018 ◽  
Vol 15 (3) ◽  
Author(s):  
Nidal Jaradat ◽  
Saad Al-Lahham
Keyword(s):  
Antioxidant Activities ◽  
Past History ◽  
Culture Collection ◽  
Natural Food ◽  
Bacterial Strains ◽  
Food Preservatives ◽  
Fungal Strains ◽  
Antibacterial And Antifungal Activities ◽  
Aerial Parts ◽  
Phytochemical Profile

Abstract Background Many recent studies have shown that medicinal plants, which have been used worldwide through the past history in the folkloric medicine, harbor a significant number of novel metabolic compounds with potent pharmacological properties. In several countries, the aerial parts of the Scolymus angiospermus plant have been used as a food supply and as a folkloric medicinal plant. The current study aimed is to investigate the antimicrobial, antilipase, antioxidant activities and phytochemical profile of methanolic, hexane, aqueous and ethyl acetate fractions obtained from the aerial parts of S. angiospermus. Methods Phytochemical assessments were based on standard analytical methods. The obtained fractions were evaluated for their antioxidant capacity and their antilipase activity using 2,2-diphenyl-1-picrylhydrazyl and porcine pancreatic lipase inhibitory tests, respectively. Antimicrobial activity of the obtained fractions was evaluated using broth microdilution assay against several American Type Culture Collection bacterial and fungal strains and Methicillin-Resistant Staphylococcus aureus clinical isolate. Results Our data showed that of all obtained fractions used in the above-mentioned assays, both of methanolic and aqueous fractions, had the highest content of flavonoids (24.93 ± 2.11 and 12.21 ± 2.11 mg QUE/g, respectively) and phenolic compounds (96.28 ± 2.87 and 91.25 ± 2.63 mg of GAEq/g, respectively) as well as the best levels of both antioxidant (half maximal inhibitory concentration (IC50) 13.67 ± 1.44 and 14.69 ± 1.97 µg/ml, respectively) and antilipase (IC50 134.89 ± 1.65 and 269.15 ± 2.33 µg/ml, respectively) activities. In addition, these fractions exhibited various levels of both antibacterial and antifungal activities. Hydrophilic fractions were more potent against the investigated bacterial strains, while hydrophobic fractions were more potent against the investigated fungal strains. Conclusions The hydrophilic fractions derived from S. angiospermus have shown the best antioxidant and antilipase effects. This is may be due to the high contents of phenols and/or flavonoids. However, further investigations are essential to isolate and identify the antioxidant, antilipase and antimicrobial compounds. Our data provide significant evidence that S. angiospermus can be very useful in the prevention and treatment of various infectious and non-infectious chronic diseases and as natural food preservatives.

In vitro antimicrobial and preliminary phytochemical screening of some Samburu Anti‐diarrhoeal medicinal plants ‐ Kenya

2012 ◽  
Vol 2 (5) ◽  
pp. 217-226
Author(s):  
E. O. Omwenga ◽  
P. O. Okemo ◽  
P. K. Mbugua
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Fungal Pathogens ◽  
Antimicrobial Effect ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Fungal Strains ◽  
Local Isolate ◽  
Agar Disc

The antimicrobial effect of some selected Samburu medicinal plants was evaluated on bacterial strains like Staphylococcus aureus ‐ ATCC 20591, Bacillus subtillis ‐ Local isolate, Salmonella typhi‐ATCC 2202, Escherichia coli‐STD. 25922 and Pseudomonas aeroginosa ‐ ATCC 25852 and fungal strains like Candida albicans ATCC EK138, Aspergillus niger ATCC 16404, Aspergillusflavus‐Local isolate, Fusarium lateritium‐Local isolate, and Penicillium spp.‐ local isolate. Methanol was used as solvent for the extraction from the selected medicinal plants used by the Samburu community. The in vitro antimicrobial activity was performed by agar disc diffusion and micro‐dilution technique. The most susceptible Gram‐positive bacterium was S. aureus, while the most susceptible Gram‐negative bacterium was P. aeroginosa. The extracts of Gomphocarpus fruticosus (L) W.T. Aiton showed less activity against the bacterial strains investigated. The most active antibacterial plants were Euphorbia scarlatica S. Carter, and Euclea divinoram Hiern. Incidentally most of the extracts were inactive against the fungal strains with only a few proving to be slightly active against the C. albicans i.e. Loranthus acaciae Zucc., Kedrostis pseudogijef (Gilg) C. Jeffrey, Euclea divinoram Hiern. and Croton macrostachyus (A. Rich). Benths. The significant antimicrobial activity of active extracts was compared with the standard antimicrobials, cefrodoxima, amoxicillin and fluconazole. The MICs of the most active plants ranged from 18.75mg/ml to 37.50mg/ml. The MBCs ranged between 18.75mg/ml to75mg/ml. These results were significant at P< 0.01. The findings show that most of the medicinal plants used by the Samburu community have some significant activity on the bacterial but not fungal pathogens known to cause diarrhoea.

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