Growth and viability of juvenile humpback grouper (Cromileptes altivelis) supplemented with inorganic and organic selenium

<p>This study was conducted to determine the effects of inorganic selenium (sodium selenite) and organic selenium (selenomethionine) supplementation on growth and viability of juvenile humpback grouper (Cromileptes altivelis). The experiment was designed as a completely randomized design with eight treatments and three replications. The treatment being tested was source and dosages of selenium ie., inorganic selenium (sodium selenite) supplementation with dosages of 0.5, 1, 2, and 4 mg Se/kg diet and organic selenium (selenomethionine) with dosages of 1, 2, and 4 mg Se/kg diet. Another treatment was unsupplemented selenium. Juveniles humpback grouper at an initial average length of 6,39±0,41 cm and body weight of 4,49±0,65 g were reared in 90×40×35 cm3 aquaria and fed artificial diet (pellet) two times daily (08.00 and 16.00) at satiation. Fishes were reared for 40 days at a stocking density of 12 fish per aquarium on sea water with salinity of 30–31 ppt and temperature of 28–29 °C. The results of this study showed that the source of selenium supplementation (inorganic or organic) affected growth performance, glutathione peroxidase (GPx) enzyme activity, and blood profiles of the experimental fish. Generally, it was found that selenomethionine supplementation resulted in better fish performance than sodium selenite. In sodium selenite supplementation, survival declined with the increased dosages of Se in the diet, and the supplementation at dosage of 0,5 mg Se/kg diet showed a toxic effects. Histopatological test showed that there were damage in livers, kidneys, and intestines of fish supplemented with sodium selenite from 0.5 to 4 mg Se/kg diet. On the contrary, supplementation of selenomethionine up to 4 mg Se/kg did not show any signs of toxicity and the survival was on 86.11 to 97.22%. Feed efficiency, protein retention, lipid retention, and Se retention indicated that a dosage of 4 mg Se/kg selenomethionine supplementation was the best dosage.</p><p>Keywords: selenium, viability, growth, Cromileptes altivelis, humpback grouper</p>

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THE INFLUENCE OF STOCKING DENSITY AGAINST SURVIVAL RATE OF CATFISH (Clarias gariepinus) ON RECIRCULATION SYSTEM

Jurnal Mina Sains ◽

10.30997/jms.v4i1.1267 ◽

2018 ◽

Vol 4 (1) ◽

pp. 11

Author(s):

Anjas Adi Santoso ◽

Muarif Muarif ◽

Rosmawati Rosmawati

Keyword(s):

Water Quality ◽

Survival Rate ◽

Feed Efficiency ◽

Average Length ◽

Clarias Gariepinus ◽

Stocking Density ◽

Average Weight ◽

Randomized Design ◽

Recirculation System ◽

Completely Randomized Design

One of the technologies to keep the water quality remains optimal ias a recirculation system. This research is aimed to determine the influence of denisity of catfish (Clarias gariepinus) on the recirculation system. Fish test used is catfish fry the age of 2 weeks with average weight 0.58 g and an average length of 2.7 cm as much as 10,000 fish. The experimental research used completely randomized design with 4 treatments and 3 replications. The treatments were A (20 fish/liter of water), B (30 fish/liter of water), C (40 fish/liter of water) and D (50 fish/liter of water). The survival rate, the feed efficiency, and water quality has been observed. The results of research showed that the survival rate and the feed efficiency were significantly different (P<0.05). The highest survival rate in this research was in the treatment A (20 fish/liter of water) that was 70.5%. The stocking density of catfish fry of 20 fish/liter of water can be used as basis for the maintenance of catfish fry for reach the optimal survival rate. Key words: Catfish, survival rate, feed efficiency, recirculation

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EFFECT OF DIFFERENT STOCKING DENSITY TO GROWTH RATE OF CATFISH (Clarias gariepinus, Burch) CULTURED IN BIOFLOC AND NITROBACTER MEDIA

AQUASAINS ◽

10.23960/aqs.v6i2.p583-588 ◽

2018 ◽

Vol 6 (2) ◽

pp. 583 ◽

Cited By ~ 1

Author(s):

Elfa Verda Puspita ◽

Ratih Purnama Sari

Keyword(s):

Growth Rate ◽

Average Length ◽

Clarias Gariepinus ◽

Stocking Density ◽

Water Volume ◽

Average Weight ◽

Market Demand ◽

Feed Conversion ◽

Randomized Design ◽

Completely Randomized Design

Catﬁsh is one of the freshwater commodities whose demand continues to increase in Indonesia. Intensive cultivation is done to meet market demand. Intensive cultivation is done by optimizing stocking density. The aim of this research was to know the effect of different density on feed productivity, growth, feed conversion ratio (FCR) and survival of catﬁsh dumbo fry (Clarias gariepinus, Burch) in bioﬂoc and nitrobacter media. This study used a completely randomized design (RAL) with 6 treatments and 3 replications. The test animalsused catﬁsh dumbo (Clarias gariepinus, Burch) with an average weight of 0,6 ± 0,1g and an average length of 4 cm. Catﬁsh dombo seeds are kept in tarpaulin ponds 2 m in diameter with water volume ± 1 m3 for 45 days and feeding 3% of biomass weight. The results showed that the different density of stockingin bioﬂoc and nitrobacter media had increase on productivity, speciﬁc growth rate, feed efﬁciency and no signiﬁcant effect on the survival of catﬁsh dumbo fry.

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KINERJA PERTUMBUHAN DAN EFISIENSI PAKAN IKAN Tor tambroides YANG DIBERI PAKAN KOMERSIAL DENGAN KANDUNGAN PROTEIN BERBEDA

Media Akuakultur ◽

10.15578/ma.12.1.2017.27-33 ◽

2017 ◽

Vol 12 (1) ◽

pp. 27

Author(s):

Deni Radona ◽

Jojo Subagja ◽

Irin Iriana Kusmini

Keyword(s):

Feed Efficiency ◽

Average Length ◽

Protein A ◽

Stocking Density ◽

Feed Conversion ◽

Protein Levels ◽

Randomized Design ◽

Completely Randomized Design ◽

Feed Protein ◽

C 50

Protein merupakan nutrien yang sangat berperan dalam pertumbuhan ikan. Penelitian ini bertujuan mengetahui kebutuhan protein optimal untuk pertumbuhan ikan Tor tambroides dan pengaruh kandungan protein pakan terhadap efisiensi pakannya. Benih ikan Tor yang digunakan berukuran panjang (1,5 ± 0,1 cm) dan bobot (0,08 ± 0,01 g). Benih ikan dipelihara dalam akuarium berukuran 40 cm x 30 cm x 30 cm dengan ketinggian air 20 cm sebanyak 50 ekor. Selama 40 hari pemeliharaan benih ikan diberi pakan komersil berupa crumble dengan kandungan protein, (A) 25%, (B) 35%, dan (C) 50% sebanyak 20% per hari dari total biomassa ikan, pemberian pakan dengan frekuensi tiga kali sehari. Rancangan penelitian menggunakan rancangan acak lengkap dengan tiga perlakuan dan tiga ulangan. Hasil penelitian menunjukkan ikan Tor tambroides yang diberi pakan dengan kandungan protein sebesar 35% dan 50% memiliki pertumbuhan panjang, bobot, laju pertumbuhan harian (LPH), biomassa, nisbah konversi pakan (FCR), dan efisiensi pakan yang sama (P>0,05) dan berbeda nyata pada pakan dengan kandungan protein 25% (P<0,05).Protein is a nutrient is which plays a major role in the growth of fish. This study was aimed to determine the optimal protein requirement for growing of Thai mahseer and the influence of different protein levels of feed on feed efficiency. The average length and weight of fish used were 1.5 ± 0.1 cm and 0.08 ± 0.01 g. The fish were reared in the aquarium (dimension= 40 cm x 30 cm x 30 cm filled with 20 cm of water and the stocking density of each aquarium was 50 individuals. During 40-day reared, seedling fish were fed by commercial crumble with different protein levels, (A) 25%, (B) 35%, and (C) 50%, as much as 20% of total weight every day with a feeding frequency of three times per day. This experiment was conducted by using completely randomized design (CRD) with three treatments and three replications for each treatment. The results showed that Tor tambroides fed diets with protein levels of 35% and 50% was not significantly different on the growth value (length and weight), specific growth rate, biomass, feed conversion ratio (FCR), and feed efficiency (P>0.05) and was significantly different on feed protein levels 25% (P<0.05).

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The Effect of Dietary Supplementation with Inorganic or Organic Selenium on the Nutritional Quality and Shelf Life of Goose Meat and Liver

Animals ◽

10.3390/ani11020261 ◽

2021 ◽

Vol 11 (2) ◽

pp. 261

Author(s):

Zabihollah Nemati ◽

Kazem Alirezalu ◽

Maghsoud Besharati ◽

Benjamin W. B. Holman ◽

Mohammadreza Hajipour ◽

...

Keyword(s):

Phenolic Content ◽

Storage Period ◽

Thiobarbituric Acid ◽

Basal Diet ◽

Organic Selenium ◽

Randomized Design ◽

Inorganic Selenium ◽

Completely Randomized Design ◽

Tbars Values ◽

Dietary Treatments

Ninety-six male goslings were allocated and assigned to treatment using a completely randomized design. Dietary treatments included a basal diet consisting of corn, wheat, and soybean meal with either no additional selenium (CON), 0.3 mg/kg of inorganic selenium (I-Se; sodium selenite), or 0.3 mg/kg of organic selenium (O-Se; selenium-enriched yeast). After a 56-day feeding period, geese were slaughtered on a common ending day and two geese per pen (n = 24) were used for the analyses conducted in this study. Meat (equal portions of the breast and thigh meat) and liver were collected and evaluated for proximate composition, fatty acid profile, pH, phenolic content, thiobarbituric acid reactive substances (TBARS), and total volatile basic nitrogen (TVB-N) over a 9-day storage period at 4 °C. The meat and liver samples from geese supplemented I-Se or O-Se had greater (p < 0.01) lipid content compared with geese not supplemented with additional selenium. At the conclusion of the 9-day storage period, meat and liver samples from geese supplemented I-Se or O-Se had lower (p < 0.05) pH values, greater (p < 0.05) phenolic content, lower (p < 0.05) TBARS values, and lower (p < 0.05) TVB-N compared with geese not supplemented with additional selenium (CON).

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Cellulase hydrolyzed Indigofera zolingeriana leaf utilization as a feed ingredient for gourami fingerling

Jurnal Akuakultur Indonesia ◽

10.19027/jai.20.2.139-147 ◽

2021 ◽

Vol 20 (2) ◽

pp. 139-147

Author(s):

Jefry Jefry ◽

Mia Setiawati ◽

Dedi Jusadi ◽

Ichsan Achmad Fauzi

Keyword(s):

Growth Performance ◽

Feed Efficiency ◽

Specific Growth ◽

Second Step ◽

Feed Ingredient ◽

Protein Retention ◽

Cellulase Enzyme ◽

Randomized Design ◽

Best Value ◽

Completely Randomized Design

This study was aimed to evaluate the utilization of hydrolyzed Indigofera zolingeriana by celullase enzyme as the feed ingredient of gourami fish. This study used a completely randomized design which contained three steps, whereas each step contained four treatments and four replications. The first step performed by evaluating the Indigofera leaf meal (ILM) added with cellulase enzyme of 0 g/kg (control), 0.4 g/kg, 0.8 g/kg, and 1.2 g/kg. The second step was the digestibility test of ILM on gourami seeds. The third step was feed evaluation added with ILM as much as 0% (control), 15%, 30%, and 45% against the growth performance on gourami seeds. The gourami seeds used in the second and third steps with a weight of 13.65 ± 0.39 g/seed and 5.95 ± 0.15 g/seed, respectively. The addition of 0.8 g/kg and 1.2 g/kg cellulase enzyme could significantly decrease the crude fiber of ILM with 43.33%, besides having the best value of total, ingredient, protein, lipid, and energy digestibility. The growth performance of gourami seeds given 15% ILM added feed had the best value and insignificantly different from the control feed without ILM addition based on the specific growth rate (SGR), protein retention (PR), and feed efficiency (FE). Keywords: Cellulase, feed, hydrolyze, Indigofera zolingeriana, Osphronemus gouramy. ABSTRAK Penelitian ini dilakukan untuk mengevaluasi pemanfaatan daun Indigofera zolingeriana yang dihidrolisis enzim selulase sebagai bahan baku pada pakan benih ikan gurami. Penelitian ini menggunakan rancangan acak lengkap yang terdiri atas tiga tahap, dimana masing-masing tahap terdiri dari empat perlakuan dan empat ulangan. Pada tahap pertama dilakukan evaluasi tepung daun Indigofera (TDI) yang ditambahkan enzim selulase sebesar 0 g/kg (kontrol), 0.4 g/kg, 0.8 g/kg, dan 1.2 g/kg. Pada tahap kedua dilakukan uji kecernaan bahan TDI pada benih ikan gurami. Pada tahap ketiga dilakukan evaluasi pakan yang ditambahkan TDI sebesar 0% (kontrol), 15%, 30% dan 45% terhadap kinerja pertumbuhan pada benih ikan gurami. Benih ikan gurami yang digunakan pada penilitian tahap kedua dengan bobot 13.65 ± 0.39 g/ekor dan 5.95 ± 0.15 g/ekor. Penambahan enzim selulase pada dosis 0.8 g/kg dan 1.2 g/kg secara signifikan mampu menurunkan serat kasar TDI sebesar 43.33 % dan memberikan nilai terbaik terhadap nilai kecernaan total, kecernaan bahan, kecernaan protein, kecernaan lemak dan kecernaan energi. Kinerja pertumbuhan benih ikan gurami yang diberikan pakan yang ditambahkan TDI sebesar 15% memilki nilai terbaik dan tidak berbeda nyata terhadap pakan kontrol tanpa TDI dari aspek laju pertumbuhan spesifik (LPS), retensi protein (RP) dan efisiensi pakan (EP). Kata kunci: Hidrolisis, Indigofera zolingeriana, Osphronemus gouramy, pakan, selulase.

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Methionine plus cystine requirements for the maintenance and efficiency of utilization with tambaqui of different body weights

Ciência e Agrotecnologia ◽

10.1590/1413-7054202044014920 ◽

2020 ◽

Vol 44 ◽

Author(s):

Neliane Galvão Porto ◽

Felipe Barbosa Ribeiro ◽

Jefferson Costa de Siqueira ◽

Marcos Antonio Delmondes Bomfim ◽

Rafael Silva Marchão ◽

...

Keyword(s):

Amino Acid ◽

Control Treatment ◽

Native Fish ◽

Amino Acid Requirement ◽

Treatment Groups ◽

Protein Retention ◽

Lack Of Information ◽

Randomized Design ◽

Body Weights ◽

Completely Randomized Design

ABSTRACT Methionine is an essential amino acid, and generally, the first limiting one in the diets of tambaqui, the main native fish produced in continental aquaculture in South America. However, there is a lack of information on their amino acid requirement, especially for maintenance and efficiency of utilization. The present study aimed to determine the requirement of [methionine plus cystine] for the maintenance and efficiency of utilization using tambaqui of different body weights. Two experiments were carried out using 250 tambaqui with weights of 100 and 300 g. They were distributed in a completely randomized design with four treatment groups (0.264, 0.396, 0.528, and 0.660% of digestible [Met + Cys]), five replications. The control treatment (CT) was performed with the addition of methionine at the first level (0.264%) until the second level was reached (0.396%). The maintenance requirement was determined by the regression analysis between the consumption of [Met + Cys] and protein retention equal to zero. The efficiency was determined using the value of the slope coefficient of the line obtained by the linear regression. The protein and methionine retention values of tambaqui weighing 100 to 300 g were compared by the parallelism test, and no difference was observed between the parameters of the equations indicating the need for only one equation to describe the responses to body weights. The requirement of digestible [Met + Cys] for the maintenance of tambaqui with weights from 100 to 300 g was 60.47 mg kg-0.7 day-1 and the efficiency of utilization of 42%.

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PENGARUH SUBSTITUSI TEPUNG IKAN DENGAN TEPUNG KEPALA IKAN PATIN (Pangasius sp) TERHADAP PERTUMBUHAN IKAN LELE SANGKURIANG (Clarias sp)

Jurnal Akuakultur Rawa Indonesia ◽

10.36706/jari.v6i2.7157 ◽

2018 ◽

Vol 6 (2) ◽

pp. 129-140

Author(s):

Yesica Manullang ◽

Limin Santoso ◽

Tarsim .

Keyword(s):

Growth Rate ◽

Fish Meal ◽

Daily Growth Rate ◽

Daily Growth ◽

Protein Retention ◽

Weight Growth ◽

Randomized Design ◽

Absolute Growth ◽

Completely Randomized Design ◽

Difference Test

ABSTRACTThe research was conducted at the Aquaculture Laboratory, University of Lampung in May to July, 2018. The aim of this study is to determine the effect of substitution of fish meal with catfish head flour on the growth of catfish, and head flour dosage the best for catfish growth. The study used a Completely Randomized Design with 3 treatments and 3 replications A (100% fish meal), B (70% fish meal and 30% catfish flour), and C (40% fish meal and 60% catfish flour). Parameters observed included absolute growth, daily growth rate, protein retention, feed convertion ratio (FCR), survival rate (SR), and supporting parameters that is feed cost and water quality. The data obtained were analyzed by variance and continued with smallest real difference test with a 95% confidence level. The results showed that the proportion of catfish head flour in artificial feed had an effect on absolute weight growth and daily growth rate (P, 0.001). The proportion of the use of 30% starch in the feed of catfish has a real influence on absolute growth (40.25 ± 5.67 g), and daily growth rate (0.67 ± 0.09 g/day).Keywords : Catfish, catfish head waste, substitution, feed, growth

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Effect of stocking density on performance, carcass yield, productivity, and bone development in broiler chickens Cobb 500®

Semina Ciências Agrárias ◽

10.5433/1679-0359.2017v38n4supl1p2705 ◽

2017 ◽

Vol 38 (4Supl1) ◽

pp. 2705

Author(s):

Cleoneide Dos Santos Henrique ◽

Andréia Fróes Galuci Oliveira ◽

Thales Silva Ferreira ◽

Eduardo Santos Silva ◽

Bruna Finotti Fonseca Reis de Mello ◽

...

Keyword(s):

Weight Gain ◽

Feed Intake ◽

Broiler Chickens ◽

Bone Development ◽

Stocking Density ◽

Live Weight ◽

Feed Conversion ◽

Randomized Design ◽

Total Phase ◽

Completely Randomized Design

This study aimed to assess the effect of stocking density of 10, 12, 14, and 16 birds m?2 on performance, carcass and cut yields, productivity, and bone development of broiler chickens Cobb 500®. The experimental design was completely randomized design with four treatments (stocking densities) and four replications. The assessments were performed weekly at 1, 7, 14, 21, 28, 35, and 42 days. The best live weight result was found at a density of 10 birds m?2 in the period from 1 to 7 days. No effect of density was observed on feed intake and weight gain. From 1 to 14 days, the best live weights were found at densities of 10, 12, and 14 birds m?2, in addition to a higher feed intake at densities of 10 and 12 birds m?2. Weight gain was better at a density of 10 birds m?2, showing no differences from densities of 12 and 14 birds m?2. In the period from 1 to 21 days, live weight and weight gain were higher at densities of 10, 12, and 14 birds m?2. The highest feed intake was observed at a density of 10 birds m?2, which showed no difference from the density of 14 birds m?2. A difference (P < 0.05) was observed for live weight, weight gain, and feed intake of animals reared at densities of 10, 12, and 14 birds m?2 for the phases from 1 to 35 days and the total phase from 1 to 42 days of bird age when compared to 16 birds m?2, which presented lower performance. However, feed conversion of 1-7, 1-14, 1-21, 1-35, and 1-42 days and carcass and cut characteristics showed no significant differences for all treatments. On the other hand, productivity was higher in the treatments with 14 and 16 birds m?2, but without difference between each other. No interaction (P > 0.05) was observed between stocking density and age for the assessed bone parameters. Similarly, no difference (P > 0.05) was observed for bone development of broiler chickens reared at different stocking densities for the studied parameters. Thus, the increased density of 10 or 12 birds m?2 to 14 birds m?2 has no influence on zootechnical performance, carcass and cut yields, and bone development, but increases broiler chicken productivity reared in a conventional shed without air conditioning.

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Stocking densities and feeding strategies in shrimp and tilapia polyculture in tanks

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2013000800039 ◽

2013 ◽

Vol 48 (8) ◽

pp. 1088-1095 ◽

Cited By ~ 7

Author(s):

Bruno Rodrigo Simão ◽

Luis Otavio Brito ◽

Alex Sandro Campos Maia ◽

Laizy Cabral Miranda ◽

Celicina Maria da Silveira Borges Azevedo

Keyword(s):

Oreochromis Niloticus ◽

Feeding Strategies ◽

Feed Conversion ◽

Shrimp Culture ◽

Main Crop ◽

Randomized Design ◽

Marine Shrimp ◽

Completely Randomized Design ◽

Feed Conversion Rate ◽

Fish Performance

The objective of this work was to evaluate the performance of Pacific marine shrimp (Litopenaeus vannamei) and tilapia (Oreochromis niloticus), in a polyculture in tanks subjected to different stocking densities and feeding strategies, in comparison with monoculture. Two experiments were performed, at the same time, in a completely randomized design with three treatments and four replicates each. Treatments for experiment I were: monoculture with 10 shrimp per m² (10S:0T); polyculture with 10 shrimp and 0.5 tilapia per m² (10S:0.5T); and polyculture with 10 shrimp and 1 tilapia per m² (10S:1T). Shrimp was the main crop, and feed was provided based on shrimp biomass. Treatments for experiment II were: monoculture with 2 tilapia per m² (2T:0S); polyculture with 2 tilapia and 2.5 shrimp per m² (2T:2.5S); and polyculture with 2 tilapia and 5 shrimp per m² (2T:5S). Tilapia was the main crop, and feed was provided based on fish requirements. In the experiment I, tilapia introduction to shrimp culture resulted in lower shrimp growth and poor feed conversion rate. In experiment II, shrimp introduction to tilapia culture did not interfere with fish performance. Polyculture is more efficient with the combination of 2 tilapia and 2.5 or 5 shrimp per m² and feed based on fish requirements.

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Comparative evaluation of influence of dietary organic and inorganic selenium supplement on growth performance of indigenous Aseel chickens

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.7608 ◽

2017 ◽

Author(s):

Waseem Muhammad Zia ◽

Anjum Khalique ◽

Saima Naveed ◽

Jibran Hussain ◽

...

Keyword(s):

Growth Performance ◽

Sodium Selenite ◽

Block Design ◽

Control Diet ◽

Nutrient Utilization ◽

Major Influence ◽

Feed Conversion ◽

Final Body Weight ◽

Organic Selenium ◽

Inorganic Selenium

Two hundred-forty 3-weeks-old Aseel birds from Lakha, Mushki, Peshaweri and Mianwali varieties of indigenous Aseel were chosen (60 birds/variety, 30 males and 30 females) to evaluate the influence of selenium supplements on growth performance. The birds of either sex were divided according to randomized complete block design into A, B and C treatment groups (10 birds/treatment); A and B were experimental, while C was control. Se-enriched yeast (organic selenium) and sodium selenite (inorganic selenium) were supplemented @ 0.3 mg/kg in the basal diets of group A and B, respectively, while, group C was fed without additional selenium. Birds were maintained individually in battery cages from 4-21 weeks. Statistically, the results showed the reduced (P>0.05) feed intake, while enhanced (P£0.05) nutrient utilization for dry matter, crude protein, crude fiber, crude fat and ash; superior feed conversion ratio; higher live final body weight; lower (P£0.05) mortality and rearing cost in Se-enriched yeast fed birds, chiefly in the males of Lakha variety than those received sodium selenite supplemented or control diet. The study concluded that the organic selenium supplement (Se-enriched yeast) had a major influence in improving the overall growth performance of Aseel.

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