scholarly journals The Effectiveness of Vitamin D Addition to Decreasing Blood Glucose Levels of Hyperglycemic Mice Model with Glimepirid Therapy

2020 ◽  
Vol 6 (2) ◽  
pp. 71
Author(s):  
Tsintani Nur Aristiana ◽  
Cholis Abrori ◽  
Muhammad Ali Shodikin
Keyword(s):  
Diabetes Mellitus ◽  
Vitamin D ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Negative Control ◽  
Control Group ◽  
Mice Model ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract Low 25-hydrocyvitamin D (25(OH)D3) levels are usually found in patients with type 1 and type 2 diabetes mellitus. This study aims to compare the effectiveness of fasting blood glucose levels in hyperglycemic mice model with glimepirid single therapy, hyperglycemic mice model with vitamin D, and mice model of hyperglycemic with glimepirid therapy plus vitamin D. This study used the true experimental research design with the randomized posttest only control group design. There were 25 male mice (Mus musculus) strain Balb/C with 20-30 g body weight and 2-3 months old which were grouped into 5 groups. The negative control group (K1) was injected with placebo and the positive control group (K2) was injected with STZ 150 mg/kgBW i.p. Groups P1, P2, and P3 were given STZ injection of 150 mg/kgBW i.p and given glimepirid 0.26 mg/kgBW; vitamin D 6,5 ml/kgBW; glimepirid 0,26 mg/kgBW with vitamin D supplementation 6,5 ml/kgBW. The results of the Tukey post hoc tests obtained differences in the mean of delta KGD between groups P1 and K2 = 122,55; in group P2 with K2 = 81,60; and in group P3 with K2 = 74,40. From these data, it can be concluded that in the group given glimepirid plus vitamin D there was no additional effectiveness in decreasing fasting blood glucose levels of hyperglycemic mice model compared to the group given only glimepirid therapy and the group given vitamin D alone. Keywords: diabetes mellitus, vitamin D, glimepiride, streptozotocin.

scholarly journals UJI AKTIVITAS ANTIDIABETES EKSTRAK ETANOL BUAH CABAI MERAH ( Capsicum annuum L. ) TERHADAP MENCIT PUTIH JANTAN

2019 ◽  
Vol 9 (1) ◽  
pp. 86 ◽  
Author(s):  
Zulkarni Zulkarni
Keyword(s):  
Blood Glucose ◽  
Capsicum Annuum ◽  
Fasting Blood Glucose ◽  
Ethanol Extract ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Capsicum Annuum L ◽  
Blood Glucose Levels ◽  
Significant Difference

This Research was conducted to determine the effect of ethanol extract from red chilli (Capsicum annuum L)in lowering blood glucose levels of hyperglycemic male white mices. This study used 30 malewhitemices and divided into 6 groups: negative control group, the positive control group, the treatmentgroup withdosage of 200 mg / kgweight, 400 mg / kgwieght, 600 mg / kg weight and a comparison group with glibenclamide with dosage of 5 mg / kgweightadministered orally for 21 days. The level of fasting blood glucose was checked 6 days after dexamethasone induced, and after the ethanol extract of red chilies on day 7th, 14th, and 21st. The data was analyzed statistically with one –way and two-way Anova by usingSPSS16 program and proceed with the test Duncan to look the significant difference between treatments. The results showed that the ethanol extract from red chilies with a dosage of 200mg / kgweight, 400mg / kgweight, 600 mg / kgweight showed the effect in lowering blood sugar levels in male white mices hyperglycemia significantly (p <0.05). The duration ethanol extract of red chili affected blood glucose levels. The most effective duration in lowering blood glucose levels is the administration of a preparation within 21 days.

scholarly journals PENGARUH EKSTRAK BAWANG HITAM TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA MENCIT

2021 ◽  
Vol 4 (1) ◽  
pp. 49
Author(s):  
Pipit Festi W
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Mus Musculus ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Significance Level ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Reduce Blood Glucose

 Administration of oral hypoglycemic drugs and insulin is one of the most common pharmacological treatments given to people with diabetes mellitus. This treatment is carried out in the long term so that it causes side effects and is carried out continuously so that it will affect the financial condition of the diabetes mellitus patient. The purpose of this study was to determine the effect of black garlic extract on decreasing blood glucose levels in mice (mus musculus). This study uses the design of True Experimental design (pretest-posttest with control group design). The population is mice in Pusvetma Surabaya. Sampling method uses Non Probability Sampling Purposive Sampling type. Samples taken were 30 mice consisting of 15 intervention groups and 15 control groups. Data was collected using observation sheets, then analyzed using Independent sample t test and Dependent sample t test with a significance level of 5 0.05. The results showed: Using the test Independent sample T test obtained a significance value ρ = 0,000  It can be concluded that there is an effect of black onion extract to reduce blood glucose levels in mice. In the paired t-test test in the treatment group showed ρ = 0,000  that there were differences in blood glucose levels before being given extracts of garlic and after being given intervention. It is expected that black garlic can be used as an alternative medicine to reduce blood glucose levels.Keyword : Ekstrak of black garlic, Blood sugar levels, mus musculus

scholarly journals The Potential of Strawberry, Rome Beauty Apple, and New Combination on Fasting Blood as Supporting Diet Therapy in Patients with Type II Diabetes Mellitus

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Niken Yuliwati ◽  
Riezky Faisal Nugroho
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Type Ii ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Post Test ◽  
Post Hoc ◽  
Diabetes Melitus

Strawberries (Fragaria x ananassa) and Rome beauty apples (Malus sylvestris) contain high antioxidants to reduce oxidative stress and improve the glycemic response with diabetes mellitus (DM) type II. This study aimed to analyze the effect of strawberries, Rome beauty apples, and their combination to decrease fasting blood glucose levels of type II DM patients. This experimental research used a randomized pre-post-test with a control group design. Subjects were 44 patients divided into four groups: K (control, not given the fruit), P1 (strawberries 200 g/day), P2 (apples 300 g/day), and P3 (the combination strawberries 100 g/day, apples 150 g/day) for 14 days in February–March 2019 in Surakarta. The inclusion criteria were DM patients aged 40–55 years who received oral antidiabetic drug therapy. Exclusion criteria were type II DM patients who, based on the doctor's diagnosis, had complications such as gastritis, hypertension, chronic renal failure, heart disease, liver disease, stroke, cancer, patients with consumption of antioxidant supplements. Mean of blood glucose level on K (149.82±39.125), P1 (141.00±38.079), P2 (128.73±31.841), and P3 (177.73±50.176). Analysis paired t test results showed a significant difference between before and after treatment (p=0.045). To find out which group was the most meaningful, the Bonferroni post hoc test. Decrease of fasting blood glucose levels with group K (−10.55, p>0.05), P1 (−30.18, p<0.05), P2 (40.27, p<0.05), and P3 (−32.91, p<0.05). ANOVA test was used to analyze, and the post hoc Bonferroni test with a control comparison was performed, resulting in p=0.028, in P2 and p>0.05 in P1 and P3. In conclusion, strawberries, Rome beauty apples, and their combination significantly decreased fasting blood glucose levels of type II DM patients, with the highest and most significant decrease in Rome beauty apples. POTENSI STROBERI, APEL ROME BEAUTY, DAN KOMBINASI BARU TERHADAP GULA DARAH PUASA SEBAGAI PENUNJANG TERAPI DIET PADA PASIEN DIABETES MELITUS TIPE IIStroberi (Fragaria x ananassa) dan apel Rome beauty (Malus sylvestris) mengandung antioksidan tinggi untuk mengurangi stres oksidatif dan memperbaiki respons glikemik pada diabetes melitus (DM) tipe II. Tujuan penelitian adalah menganalisis pengaruh buah stroberi, apel Rome beauty, dan kombinasinya terhadap penurunan kadar glukosa darah puasa pasien DM tipe II. Jenis penelitian eksperimen dengan rancangan randomized pre-post-test dengan control group design. Subjek penelitian 44 pasien yang dibagi empat kelompok, yaitu K (kontrol tidak diberi buah), P1 (stroberi 200 g/hari), P2 (apel Rome beauty 300 g/hari) dan P3 (kombinasi stroberi 100 g/hari dan apel 150 g/hari) selama 14 hari pada bulan Februari–Maret 2020 di Surakarta. Kriteria inklusi adalah pasien DM usia 40–55 tahun yang mendapat terapi obat antidiabetik oral. Kriteria eksklusi adalah pasien DM tipe II yang berdasar atas diagnosis dokter mengalami komplikasi seperti mag, hipertensi, gagal ginjal kronik, penyakit jantung, penyakit hati, strok, kanker, dan pasien dengan konsumsi suplemen antioksidan. Kadar glukosa darah rerata pada K (149,82±39,125), P1 (141,00±38,079), P2 (128,73±31,841), dan P3 (177,73±50,176). Hasil analisis paired t test menunjukkan terdapat perbedaan yang signifikan antara sebelum dan sesudah perlakuan (p=0,045). Untuk mengetahui kelompok mana yang paling bermakna, dilakukan Uji Post Hoc Bonferroni. Penurunan kadar glukosa darah puasa pada K (−10,55; p>0,05), P1 (−30,18; p<0,05), P2 (−40,27; p<0,05), dan P3 (−32,91; p<0,05). Analisis dilakukan dengan uji ANOVA dan Uji Post Hoc Bonferroni dengan perbandingan kontrol menghasilkan p=0,028 pada P2 serta p>0,05 pada P1 dan P3. Simpulan, pemberian stroberi, apel Rome beauty, dan kombinasinya signifikan menurunkan kadar glukosa darah puasa pasien DM tipe II dengan penurunan tertinggi dan bermakna pada apel Rome beauty.

scholarly journals Uji Efektivitas Beras Herbal Forte terhadap Penurunan Kadar Glukosa Darah Pasien Diabetes Melitus Tipe 2 (Study of the Herbal Rice Forte Effectiveness on Decreasing Blood Glucose of Diabetes Mellitus Type 2 Patient)

2018 ◽  
Vol 6 (1) ◽  
pp. 85
Author(s):  
Gema Akbar Wakhidana ◽  
Ancah Caesarina Novi Marchianti ◽  
Ali Santosa
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Treatment Group ◽  
Metabolic Diseases ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Type 2 Dm

Diabetes mellitus is metabolic diseases characterized by hyperglycemia caused by disturbances in insulin secretion or insulin activity. Herbal Forte Rice is rice synthetic material composed of main purple sweet potato and corn flour. It contains antioksidan, fiber, resistant starch and having moderate glycemic index. This study aimed to determine the effectivity of Herbal Forte Rice on decreasing blood glucose levels of patients type 2 DM. It used a cross-over design, the role of research subjects was both as control and treatment groups. Total sample of this research consisted of 60 samples. The treatment group was given Herbal Forte Rice for 7 days in 2 consecutive meals, while the control group did not receive any intervention. The results showed the average level of Fasting Blood Glucose (FBG) beginning and end of the treatment group 179.76 mg/dL and 138.7 mg/dL; FBG beginning and end of the control 180.87 mg/dL and 187.63 mg/dL; 2 hours post prandial Blood Glucose (2hppBG) levels of beginning and end of the treatment group 284.37 mg/dL and 183.8 mg/dL; 2hppBG levels beginning and end of the control 280.57 mg/dL and 284.13 mg/dL. This study concludes that Herbal Forte Rice effective on decreasing blood glucose levels of patients type 2 DM.   Keywords: diabetes mellitus, Rice Herbal Forte, GDP and GD2PP  

scholarly journals Pengaruh Pemberian Minuman Fungsional Terhadap Penurunan Kadar Glukosadarah Puasa Pada Penderita Diabetes Melitus

2019 ◽  
Vol 4 (2) ◽  
pp. 114
Author(s):  
Tutut Zikra Anjani ◽  
Suhaema Suhaema ◽  
Fifi Lutfiah ◽  
Ni Ketut Sri Sulendri
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Female Gender ◽  
Simple Random Sampling ◽  
Control Group ◽  
Risk Category ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Post Test

Indonesia is currently the fourth country with the largest number of DM patients in the world. Functional food is beneficial for the health of DM patients, for example bananas and soybeans. Kepok bananas contain dietary fiber inulin and antioxidants (β carotene). Soybeans contain isoflavones, fiber, and proteins in the form of amino acids arginine and glycine. This study aims to look at the effect of giving functional drinks a mixture of soybean and banana milk on decreasing fasting blood glucose levels in type II DM patients. Type of Quasy-experimental research with pre test and post test with control group design. Subjects were taken using the Simple Random Sampling method and the subjects involved until the end of the study were 26 people. The intervention was carried out for 14 days and given as much as 330 ml. The equality of the subject characteristics of the two groups was analyzed using the chy squere test, and the effect of giving intervention materials on decreasing blood glucose levels was tested by independent t test. The results of the study were characteristics (84.6%) aged 50-60 years, (53.8%) female gender, (57.1%) having a family history of disease, 58.4% using glibenclamide anti-hyperglycemic drugs and BMI average in the risk category. . Result Δ It is known that there is an effect of giving functional drinks on decreasing fasting blood glucose levels where the average change is 11.5 mg / dl (p = 0.03). 

scholarly journals The Effectivity of Tomato and Guava Combination Juice and Guava Juice Administration on Blood Glucose Level in Patients with Type II Diabetes Mellitus

2019 ◽  
Author(s):  
Hasneli . ◽  
Safyanti . ◽  
Ainil Mardhiyah
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Health Center ◽  
Type Ii Diabetes ◽  
Fasting Blood Glucose ◽  
Type Ii Diabetes Mellitus ◽  
Control Group ◽  
Type Ii ◽  
Glucose Levels ◽  
Blood Glucose Levels

This study aims to determine the effectivity of tomato and guava juice combination with guava juice on blood glucose levels of patients with type II diabetes mellitus in the work area of Kuranji Padang Health Center in 2019. This quasi-experimental study used a pretest-posttest design with a control group. The population of this study was all patients with type II diabetes mellitus in the working area of Kuranji Health Center, Padang City. Sampling is done by purposive sampling. The number of samples was 24 people, divided into treatment groups and a control group. The data obtained were analyzed by two different dependent tests and an independent t-test. The results of statistical tests showed that there were significant differences between the average decrease in fasting blood glucose levels of respondents who were given tomato and guava combination juice with respondents who were given guava juice (p = 0,026). People with diabetes mellitus are expected to consume tomato and guava combination juice as a form of complementary therapy.

Daya Antihiperglikemia Yoghurt Pada Mencit Putih Jantan

2019 ◽  
Vol 4 (2) ◽  
pp. 72
Author(s):  
Irene Puspa Dewi ◽  
Roza Septriani ◽  
Verawaty Verawaty ◽  
Dwi Mulyani
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Percentage Increase ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference

<p><em>Research has been conducted on the antihyperglycemic of yogurt in male white mice. Yogurt contains probiotics that have a positive effect on both type 1 and types 2 diabetes mellitus. Yogurt is given for 3 weeks at a dose of 200 grams / Kg BW / day and 400 grams / Kg BW / day to mice that have been alloxan-induced. As a comparison, a positive control group was created, the alloxan-induced mice group, but not given yogurt and a negative control group, the mice group without any treatment. After 3 weeks of treatment with yogurt, it was found that there was a significant difference between the average blood glucose levels in the mice of the 200 grams/ Kg BW group and the 400 grams / Kg BW dose with the positive control group. From the data on the percentage increase in blood glucose levels of mice, it is known that by giving yogurt for 3 weeks, reducing the high percentage of increase in blood glucose levels in mice. From these data, it can be concluded that the administration of yogurt with a dose of 200 grams / Kg / day and 400 grams / Kg / day can help reduce blood glucose levels in alloxan-induced mice.</em></p><p> </p><p><em>Telah dilakukan penelitian mengenai daya antihiperglikemia yogurt pada mencit putih jantan. Yogurt mengandung probiotik  yang memberikan pengaruh positif terhadap diabetes mellitus baik tipe 1 maupun tipe 2. Yogurt diberikan selama 3 minggu dengan dosis 200/Kg BB/hari dan 400 gram/Kg BB/hari kepada mencit yang telah diinduksi aloksan. Sebagai pembanding dibuat kelompok kontrol positif yaitu kelompok mencit yang diinduksi aloksan, namun tidak diberi yogurt dan kelompok kontrol negatif, yaitu kelompok mencit tanpa perlakuan apapun. Setelah 3 minggu perlakuan dengan yogurt, didapatkan hasil bahwa terdapat perbedaan bermakna antara rata-rata kadar glukosa darah mencit kelompok dosis 200 gram/Kg BB dan dosis 400 gram/Kg BB dengan kelompok kontrol postif. Dari data persentase kenaikan kadar glukosa darah mencit, diketahui bahwa dengan pemberian yogurt selama 3 minggu, mengurangi tingginya persentase kenaikan kadar glukosa darah mencit.dari data-data tersebut, dapat disimpulkan bahwa pemberian yogurt dengan dosis 200 gram/Kg BB/hari dan 400 gram/Kg BB/hari dapat membantu menurunkan kadar glukosa darah mencit yang diinduksi aloksan.</em></p>

scholarly journals PENGARUH PEMBERIAN TRANS FATTY ACID (TFA) DARI MARGARIN DAN MINYAK KELAPA SAWIT YANG DIPANASKAN BERULANG TERHADAP KADAR GLUKOSA DARAH PUASA PADA TIKUS WISTAR

2017 ◽  
Vol 11 (1) ◽  
pp. 69
Author(s):  
Retno Larasati ◽  
Bambang Wirjatmadi ◽  
Merryna Adriani
Keyword(s):  
Fatty Acid ◽  
Blood Glucose ◽  
Trans Fatty Acid ◽  
Palm Oil ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels

The purpose of this study was to know differences in fasting blood glucose levels among both the control and treatment groups were given Trans Fatty Acid of groceries margarine and oil is heated rapidly. This research is true experimental design to study Post Test Only Control Group Design. The sample size in this study was 25 rats wistar strain males were divided into 5 groups: the first group was the control, the second group by of margarine that is heated by the content of TFA 1%, the third group by the addition palm oil, which is heated repeats to content TFA 1%, the fourth group by margarine are heated with TFA content of 2%, and the fifth group by the addition of palm oil, which is heated repeats to the TFA content of 2%. Treatment was given for 4 weeks, after the laboratory examination of fasting blood glucose at the end of the treatment. Data fasting blood glucose levels in all groups were tested by one-way ANOVA with 95% confidence level. The results showed that TFA may increase the levels of fasting blood glucose treatment groups compared with the control group, and the differences in levels of fasting blood glucose between treatment groups was not influenced by the amount of content TFA but due to different types of TFA given that of margarine and palm oil that is heated Repeat.Keywords: fasting glucose, TFA, margarine, oil heated, rats

scholarly journals Pengaruh pemberian susu almond terhadap glukosa darah puasa pada tikus diabetes

2019 ◽  
Vol 7 (2) ◽  
pp. 86-91
Author(s):  
Ryta Ristantia Ningsih ◽  
Enny Probosari ◽  
Binar Panunggal
Keyword(s):  
Blood Glucose ◽  
Treatment Group ◽  
Metabolic Diseases ◽  
Fasting Blood Glucose ◽  
Glucose Measurement ◽  
Control Group ◽  
Control Group Design ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Significant Difference

Background: Type 2 Diabetes Mellitus is a group of metabolic diseases with characteristic hyperglycemia that occurs due to abnormalities in insulin secretion and insulin action. Diet plus high fiber can help control glucose levels. Almond contains fiber, vitamin E, and magnesium that contributes to the fasting blood glucose levels. This study aimed to analyze the effect of almond milk to the fasting blood glucose diabetic rats.Method: This study used an experimental design with pre-post test control group design. The sample were Rattus norvegicus Wistar which were divided into 4 groups: K (-), K (+), A1 and A2. Almond milk was given in the group treatmeant for 14 days with a dose of 4.32 ml and 8.64 ml. Fasting blood glucose measurement taken before and after treatment used Oxidase Glucose - Peroxidase Aminoantypirin (GOD PAP). Analysis of data used paired t-test, ANOVA and Post Hoc.Results: The fasting blood glucose levels decreased significantly (p = 0.01) in the treatment group which were intervened with almond milk. A decrease in fasting blood glucose levels in a dose treatment group 1 for 116.96 ± 4.45 (p = 0:01), whereas in the group treated at a dose of 2 for 155.53 ± 3.15 (p = 0.01). There were a significant difference in decreased of the fasting blood glucose levels between the two treatment groups (p = 0.01).Conclusion:  Giving a dose almond milk 4.32 ml and 8.64 ml for 14 days can lower the fasting blood glucose levels significantly.

scholarly journals Effect of Celery Extract on Fructose Induced Insulin Resistance Rattus norvegicus

2021 ◽  
Vol 56 (4) ◽  
pp. 269
Author(s):  
Devitya Angielevi Sukarno ◽  
Arifa Mustika ◽  
Purwo Sri Rejeki
Keyword(s):  
Insulin Resistance ◽  
Blood Glucose ◽  
Treatment Group ◽  
Rattus Norvegicus ◽  
Therapy Group ◽  
Fasting Blood Glucose ◽  
Negative Control ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus (DM) is one of the global health problems and in the top 4th ranks as the main cause of death in developing countries (IDF, 2015). The pathogenesis of type II DM involves abnormalities in insulin secretion and activity that leads to insulin resistance. This research aims to study the efficacy of celery (Apiumgraveolens) as a prevention of insulin resistance. In this study, the samples were 45 Wistar rats (Rattus norvegicus), male sex, aged 4-6 weeks, weight 150-175 grams,and had normal fasting blood glucose levels by tested before treatment. The experimental animals were divided into 5 groups, K1 was negative control group (insulin resistance by given 20% fructose 1,86 g/kg BW PO qDay);K2 was positive control group (those given 20% fructose 1,86 g/kg BW PO qDayand standard insulin resistance therapy metformin 500 mg/kg BW PO qDay); the K3 treatment group was given 20% fructose 1,86 g/kg BW PO qDay and celery extract 200 mg/kg BW PO qDay; the K4 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 400 mg/kg BW PO qDay; and the K5 treatment group was given fructose 20% 1,86 mg/kg BW PO qDay and celery extract 600 mg/kg BW PO qDay. The treatment had been given every day for 60 days.Fasting blood glucose levels were measured using a Glucometer. Fasting blood insulin levels were measured using ELISA, HOMA-IR was calculated using a standardized formula, and GLUT4 protein expression was measured using immunohistochemistry. It the end of the intervention, there was a significant decreased in fasting blood glucose (FBG) in K4 group compared with K1 (p <0.05), insulin resistance in K1 was characterized by a higher HOMA-IR value compared to the therapy group, especially K4 and K5 (p <0.05). There was an increase in GLUT-4 expression on K4 and K5 compared with K1 (p <0.05). It can be concluded that celery extract has antihyperglycemia effect and furthermore it can prevent insulin resistance condition.

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