scholarly journals Viabilitas Bacillus sp. Sebagai Agen Antagonis Patogen Tanaman Dalam Formulasi Berbahan Dasar Tepung

2020 ◽  
Vol 3 (1) ◽  
pp. 29
Author(s):  
Dhirham Khusma Fakhruddin ◽  
Suhartiningsih Dwi Nurcahyanti
Keyword(s):  
Rice Flour ◽  
Inhibition Zone ◽  
Bacterial Suspension ◽  
Bacillus Sp ◽  
Corn Flour ◽  
Xanthomonas Axonopodis ◽  
Inhibitory Capacity ◽  
Control Disease ◽  
Tapioca Flour

Bacterial biopesticide formulations needed because the use of bacteria in the suspensions can reduce the ability to control disease in plants. Therefore, a bacterial suspension needs to be mobilized in the formula with a carrier (Carrier) to maintain the viability of bacteria. This research uses the formulation of Bacillus sp. made from rice flour, corn, tapioca and talc with the addition of urea, glucose and CMC. Formulation of Bacillus sp. flour based with the addition of urea, glucose and CMC were tested with Xanthomonas axonopodis pv. glycines and Colletotrichum sp. in vitro. The results showed that Bacillus sp. after formulation it is still able to maintain viabiitas and antagonistic power. Viability of Bacillus sp. the best is in the formulation of rice flour at 14 hsi at 4.94 X 1014 cfu / g while the inhibition zone of Bacillus sp. the best is the corn flour formulation of 13.1 mm and the inhibitory capacity of Bacillus sp. the best is in tapioca flour formulation at 42 hsi at 62.89%

scholarly journals Biological Control of Sheath Blight Disease of Rice by using Antagonistic Bacteria

1970 ◽  
Vol 45 (3) ◽  
pp. 225-232 ◽  
Author(s):  
MA Bashar ◽  
MA Hossain ◽  
MM Rahman ◽  
MN Uddin ◽  
MN Begum
Keyword(s):  
Biological Control ◽  
Rhizoctonia Solani ◽  
Inhibition Zone ◽  
Sheath Blight ◽  
Bacterial Suspension ◽  
Antagonistic Bacteria ◽  
Causal Organism ◽  
Sheath Blight Disease ◽  
Blight Disease

The study was made to detect and identify antagonistic bacteria to control Rhizoctonia solani, a causal organism of sheath blight (ShB) disease of rice. Isolation of antagonistic bacteria was done from ShB infected rice leaf collected from the districts of Gazipur, Rajshahi, Bogra and Comilla. Fifty isolates of bacteria were isolated. These isolates were tested for antagonism against ShB pathogen of in PDA medium. Among the isolates of antagonistic bacteria (both fluorescent and non fluorescent), eleven produced more than 15 mm inhibition zone, were selected for this study. Remarkable inhibition zone producing ten isolates were selected to observe their antagonistic behaviour by soaking the sclerotia of Rhizoctonia solani and rice seedlings in different hours into bacterial suspension of 3.84 x 107 CFU/ml. Both the in vitro and in vivo rom showed that the sclerotial germination and sheath blight symptom expression were delayed 6-9 days. Soaking of both seedlings and sclerotia with the test bacteria was found most effective to control ShB (38% - 100%) than soaking of seedlings alone with bacterial suspension at different hours. However, BanShbFPS5 (2)B, BanShb738(3), BanShb738(2) and BanShb581(1), the four antagonistic bacterial isolates could be applied as biological agent to control sheath blight disease of rice and they could control sheath blight disease development and could delay the epidemics of the disease. Key words: Biological control; Sheath blight disease; Rhizoctonia solani; Antagonistic bacteria DOI: 10.3329/bjsir.v45i3.6529Bangladesh J. Sci. Ind. Res. 45(3), 225-232, 2010

scholarly journals Nanoparticles in inhibiting Pantoea ananatis and to control maize white spot

2022 ◽  
Vol 52 (7) ◽  
Author(s):  
Morgana Coelho Mamede ◽  
Raquel Pinheiro Mota ◽  
Anielle Christine Almeida Silva ◽  
Nilvanira Donizete Tebaldi
Keyword(s):  
Bacterial Growth ◽  
Inhibition Zone ◽  
White Spot ◽  
Bacterial Suspension ◽  
Bacterial Disease ◽  
Zno Nps ◽  
Pantoea Ananatis ◽  
Growth Inhibition Zone ◽  
Progress Curve

ABSTRACT: Maize white spot (MWS) caused by Pantoea ananatis is one main maize leaf diseases, and nanoparticles (NPs) are an innovative approach for bacterial disease control. This research evaluated the toxicity of pure NPs and doped NPs with different elements in inhibiting bacterial growth and to control MWS. Pure NPs and ZnO NPs doped with silver (Ag), gold (Au), copper (Cu), iron (Fe), manganese (Mn), and nickel (Ni) at different concentrations were used to determine the toxicity for P. ananatis in vitro, evaluating the bacterial growth inhibition zone. To assess the control of MWS, in the preventive application, maize plants were sprayed with NPs of ZnO:0.1Cu, ZnO:0.05Fe, ZnO:0.2Mn and ZnO:0.7Ni at 10, 5 or 2.5 mg mL-1, and after 3 days, the plants were inoculated with bacterial suspension. To assess the curative application, plants were inoculated with the bacteria, and 3 days later sprayed with the NPs. The disease severity was assessed and the area under the disease-progress curve (AUDPC) was calculated. The doped ZnO NPs with different elements, and at different concentrations inhibited bacterial growth in vitro. NPs of ZnO:0.1Cu and ZnO:0.2Mn at 5 or 2.5 mg mL-1, in both applications reduced the severity of MWS, showing potential for use in the disease management.

scholarly journals Determination and Optimization of Flour for Producing Crispy Baby Tilapia using Plackett Burman Design and Central Composite Design Method

2017 ◽  
Vol 12 (1) ◽  
pp. 1
Author(s):  
Syamdidi Syamdidi ◽  
Diah Ikasari ◽  
Hasta Octavini
Keyword(s):  
Central Composite Design ◽  
Oreochromis Niloticus ◽  
Design Method ◽  
Rice Flour ◽  
Corn Flour ◽  
Optimum Amount ◽  
Burman Design ◽  
Tapioca Flour ◽  
Plackett Burman Design ◽  
Central Composite

Research on processing of crispy baby tilapia (Oreochromis niloticus) was conducted to obtain type and proportion of flour on this product with central composite design method. This research used 6 types of flour, namely wheat flour, rice flour, potato flour, tapioca flour, corn flour and baking powder. Baby tilapia used for this research were 30-40 day old, 2-3 cm long. Parameters observed were sensory (appearance, odor, taste, texture, overall acceptance) and crispness for the physical parameter. The results showed that only two out of six variables gave big effect on the tested response i.e potato and rice flour. Those two variables were then optimized with central composite design method to obtain the best product. The optimization process demonstrated that the optimum amount of potato and rice flour were 58-60 g (22.16-22.92%) and 40-60 g (15.28-22.92%), respectively.

scholarly journals The Comparison of Metronidazole, Clindamycin, and Amoxicillin Againts Streptococcus sanguinis

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Kevin Lim ◽  
Armelia Sari Widyarman
Keyword(s):  
Brain Heart Infusion ◽  
Inhibition Zone ◽  
Bacterial Suspension ◽  
Bacterial Cells ◽  
Viridans Streptococci ◽  
Streptococcus Sanguinis ◽  
Significant Difference ◽  
Alveolar Osteitis

  Introduction: Viridans streptococci group such as Streptococcus sanguinis (S. sanguinis), an anaerobic Gram-positive bacteria is a well-known for its involvement in dry socket (alveolar osteitis)-associated infection. Systemic amoxicillin, clindamycin and metronidazole have all been shown to be effective to inhibit this bacterium. However, there has been a lack of studies identifying which are the most effective amongst these antibiotics toward Streptococcus sanguinis.   Objectives: The purpose of this study is to evaluate the effectiveness of metronidazole, clindamycin, and amoxicillin in inhibiting the growth of Streptococcus sanguinis in vitro.   Methods: This effectiveness was done by using agar well diffusion methods. S. sanguinis ATCC 10556 were cultured in Brain Heart Infusion (BHI) broth at 37°C under anaerobic condition. After 48h, bacterial cells were harvested and counted using microplate reader (490 nm) to achieve optical density of 0.25-0.30 (107 CFU/mL). Subsequently, 100 μL of bacterial suspension was cultured on BHI agar and each antibiotic suspension was added into each agar well, incubated for 72h at 37°C. The inhibition zone diameters were measured with electronic caliper. All experiments were done in triplicate, and repeated two times in separated occasions. The obtained data were statistically analyzed using one-way ANOVA test. A  p<0.05 was considered as significance.   Result: The results showed that there was a significant difference in the effectiveness, clindamycin and amoxicillin in inhibiting the growth of Streptococcus sanguinis (p<0.05), compared to metronidazole. The inhibition zone diameter with mean±SD (mm) are 13.50±2.0, 34.67±2.3 and 32.67±1.7 for metronidazole, clindamycin and amoxicillin, respectively.   Conclusion: Clindamycin and amoxicillin are more effective in inhibiting the growth of Streptococcus sanguinis compared to metronidazole in this study. However, future studies are needed to confirm this result in vivo.

scholarly journals Kerentanan Jenis Tepung terhadap Infestasi Kumbang Tepung Merah (Tribolium castaneum Herbst) (Coleoptera: Tenebrionidae)

Agrikultura ◽  
2016 ◽  
Vol 27 (3) ◽  
Author(s):  
Hendrival Hendrival ◽  
Latifah Latifah ◽  
Dedi Saputra ◽  
Orina Orina
Keyword(s):  
Population Growth ◽  
Tribolium Castaneum ◽  
Rice Flour ◽  
Potato Flour ◽  
Corn Flour ◽  
Glutinous Rice ◽  
Growth Development ◽  
Sweet Potato Flour ◽  
Tapioca Flour ◽  
Coleoptera Tenebrionidae

ABSTRACT Susceptibility of various flour types to the investation of red flour beetle ( Tribolium castaneum Herbst) (Coleoptera: Tenebrionidae) The research on the level of flour susceptibility against infestation of Tribolium castaneum has been conducted in laboratory under conditions of 28-30oC and relative humidity of 70-75%. Each type of flour was infested by 10 pairs of T. castaneum adult. Variables observation included the population growth, development period, weight losses percentage and susceptibility index. The results showed that degree of flour suitability against population growth namely wheat flour > corn flour > bran > mungbean flour > rice flour > potato flour > purple cultivar sweet potato flour > bread crumbs > glutinous rice flour > tapioca flour = sago flour. The longest median development time of T. castaneum was found in tapioca and sago flours of 86 days while the shortest was in wheat flour of 23.33 days. The highest weight loss percentage occurred on wheat and corn flours. The susceptibility level of flours to T. castaneum with resistant category included tapioca flour, corn flour, glutinous rice flour, purple cultivar sweet potato flour and bread crumbs. Mungbean flour was in moderate gategory while potato flour was in resistant to moderate category. Rice flour and bran were classified in susceptible category while corn and wheat flours were classified as highly susceptible. Keywords: Tribolium castaneum , Flour, Population growth, Development period, Susceptibility ABSTRAK Penelitian tingkat kerentanan jenis tepung terhadap infestasi Tribolium castaneum telah dilakukan di laboratorium pada kondisi suhu 28-30oC dan RH 70-75%. Setiap jenis tepung diinfestasi 10 pasang imago T . c a s t a n e u m . Variabel pengamatan meliputi pertumbuhan populasi, periode perkembangan, persentase susut berat dan indek kerentanan. Hasil penelitian menunjukkan bahwa tingkat kesesuaian jenis tepung terhadap pertumbuhan populasi yaitu tepung gandum > tepung jagung > dedak > tepung kacang hijau > tepung beras > tepung kentang > tepung ubi jalar kultivar ungu > tepung panir > tepung ketan putih > tepung tapioka = tepung sagu. Median waktu perkembangan T. castaneum paling lama dijumpai pada tepung tapioka dan sagu yaitu 86 hari, sedangkan paling singkat pada tepung gandum yaitu 23,33 hari. Susut berat tepung paling banyak terjadi pada tepung gandum dan jagung. Tingkat kerentanan jenis tepung terhadap T. castaneum yaitu katagori resisten meliputi tepung tapioka, tepung sagu, tepung ketan putih, tepung ubi jalar kultivar ungu, dan tepung panir. Katagori moderat yaitu tepung kacang hijau, sedangkan tepung kentang tergolong resisten sampai moderat. Tepung beras dan dedak tergolong katagori rentan, sedangkan tepung jagung dan gandum tergolong katagori sangat rentan. Kata Kunci: Tribolium castaneum , Tepung, Pertumbuhan populasi, Periode perkembangan, Tingkat kerentanan

scholarly journals Corn Wingko Processing Optimization Using Response Surface Methodology

2020 ◽  
Vol 1 (2) ◽  
pp. 28-33
Author(s):  
Jariyah Jariyah ◽  
Wicaksono L A ◽  
Septi N D
Keyword(s):  
Response Surface Methodology ◽  
Moisture Content ◽  
Water Content ◽  
Response Surface ◽  
Starch Content ◽  
Rice Flour ◽  
Crude Fiber ◽  
Corn Flour ◽  
Glutinous Rice ◽  
Tapioca Flour

Wingko is semi-wet food from Indonesia made from grated coconut, glutinous rice flour, sugar and other additives that form a distinctive taste and aroma. Utilization of corn in the form of flour aims to reduce the use of glutinous rice flour as the main composition of wingko because in addition to being an economic commodity, corn contains fiber which is useful for digestion. This study aims to determine the effect of the proportion of corn flour, sticky rice flour and tapioca on the physical and chemical properties of wingko. This study uses the Response Surface Methodology (RSM) method with the experimental design of the Central Composite Design (CCD) using 3 factors of 5 levels, namely the proportion of corn flour (43.18; 50; 60; 70; 76.82) (w / w), flour sticky rice (23,18; 30; 40; 50; 56,82) (w / w), tapioca flour (6,59; 10; 15; 20; 23,41) (w / w) are then processed using Design Expert software 7.1.5. The parameters observed were corn flour including water content, ash, starch, amylose, crude fiber and yield, in glutinous rice flour and tapioca including starch and amylose content, while in wingko products included water content, aw, starch, crude fiber and texture. The results showed that corn flour had a moisture content of 7.12%, ash 0.34%, starch 84.72%, amylose 21.22% of the total ingredients, crude fiber 1.15% and yield of 79.8%. Glutinous rice flour has a starch content of 81.98% and amylose 1.02% of the total ingredients while tapioca flour has a starch content of 78.71% and amylose 20.63% of the total ingredients. The optimum conditions of wingko products were obtained in the proportion of corn flour: sticky rice: tapioca 50:38:20 (w / w) with 23.46% moisture content, aw 0.881, starch content 50.87%, crude fiber 2.78% and texture 0.01194 mm / gs had desirability 0.831.

scholarly journals Effects of chitosan-collagen dressing on wound healing in vitro and in vivo assays

2021 ◽  
Vol 19 ◽  
pp. 228080002198969
Author(s):  
Min-Xia Zhang ◽  
Wan-Yi Zhao ◽  
Qing-Qing Fang ◽  
Xiao-Feng Wang ◽  
Chun-Ye Chen ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Type I Collagen ◽  
Inhibition Zone ◽  
Negative Control ◽  
Type I ◽  
Nih3t3 Cells ◽  
Post Operation

The present study was designed to fabricate a new chitosan-collagen sponge (CCS) for potential wound dressing applications. CCS was fabricated by a 3.0% chitosan mixture with a 1.0% type I collagen (7:3(w/w)) through freeze-drying. Then the dressing was prepared to evaluate its properties through a series of tests. The new-made dressing demonstrated its safety toward NIH3T3 cells. Furthermore, the CCS showed the significant surround inhibition zone than empty controls inoculated by E. coli and S. aureus. Moreover, the moisture rates of CCS were increased more rapidly than the collagen and blank sponge groups. The results revealed that the CCS had the characteristics of nontoxicity, biocompatibility, good antibacterial activity, and water retention. We used a full-thickness excisional wound healing model to evaluate the in vivo efficacy of the new dressing. The results showed remarkable healing at 14th day post-operation compared with injuries treated with collagen only as a negative control in addition to chitosan only. Our results suggest that the chitosan-collagen wound dressing were identified as a new promising candidate for further wound application.

scholarly journals Allelopathic Effects of Three Herb Species on Phytophthora cinnamomi, a Pathogen Causing Severe Oak Decline in Mediterranean Wood Pastures

Forests ◽  
2021 ◽  
Vol 12 (3) ◽  
pp. 285
Author(s):  
Manuela Rodríguez-Romero ◽  
Belén Godoy-Cancho ◽  
Isabel M. Calha ◽  
José António Passarinho ◽  
Ana Cristina Moreira
Keyword(s):  
Phytophthora Cinnamomi ◽  
Raphanus Raphanistrum ◽  
Inhibitory Capacity ◽  
Pathogen Suppression ◽  
Pathogen Populations ◽  
Defensive Chemical ◽  
Chemical Response ◽  
Herb Species

The ability of three herbaceous plants (Diplotaxis tenuifolia (L.) DC., Eruca vesicaria L. and Raphanus raphanistrum L.) from Iberian wood pastures to reduce Phytophthora cinnamomi Rands pathogen populations through allelopathic relationships is studied. The inhibitory capacity of their aqueous root extracts (AREs) on mycelial growth and production of P. cinnamomi reproductive structures is analysed in vitro. In addition, Quercus seedlings were grown in infested by P. cinnamomi-soils and with the presence or absence of allelopathic and susceptible herb species to the pathogen to assess the defensive chemical response of Quercus seedlings through their leaf phenolic compounds. Results show a strong inhibitory capacity of AREs on P. cinnamomi activity in vitro and a protective effect of these herb species on Quercus plants against P. cinnamomi in vivo. D. tenuifolia would be especially suited for biological control in the pathogen suppression.

scholarly journals THE RATE OF BACTERICIDAL ACTION OF PENICILLIN IN VITRO AS A FUNCTION OF ITS CONCENTRATION, AND ITS PARADOXICALLY REDUCED ACTIVITY AT HIGH CONCENTRATIONS AGAINST CERTAIN ORGANISMS

1948 ◽  
Vol 88 (1) ◽  
pp. 99-131 ◽  
Author(s):  
Harry Eagle ◽  
A. D. Musselman
Keyword(s):  
Bacterial Species ◽  
Fold Increase ◽  
Bactericidal Effect ◽  
Penicillin G ◽  
Bacterial Suspension ◽  
Maximal Effect ◽  
Maximal Rate ◽  
Effective Level ◽  
High Concentrations

1. The concentrations of penicillin G which (a) reduced the net rate of multiplication, (b) exerted a net bactericidal effect, and (c) killed the organisms at a maximal rate, have been defined for a total of 41 strains of α- and ß-hemolytic streptococci, Staphylococcus aureus and Staphylococcus albus, Diplococcus pneumoniae, and the Reiter treponoma. 2. The concentration which killed the organisms at a maximal rate was 2 to 20 times the minimal effective level ("sensitivity" as ordinarily defined). With some organisms, even a 32,000-fold increase beyond this maximally effective level did not further increase the rate of its bactericidal effect. However, with approximately half the strains here studied (all 4 strains of group B ß-hemolytic streptococci, 4 of 5 group C strains, 5 of 7 strains of Streptococcus fecalis, 2 of 4 other α-hemolytic streptococci, and 4 of 9 strains of staphylococci), when the concentration of penicillin was increased beyond that optimal level, the rate at which the organisms died was paradoxically reduced rather than increased, so that the maximal effect was obtained only within a relatively narrow optimal zone. 3. There were marked differences between bacterial species, and occasionally between different strains of the same species, not only with respect to the effective concentrations of penicillin, but also with respect to the maximal rate at which they could be killed by the drug in any concentration. Although there was a rough correlation between these two factors, there were many exceptions; individual strains affected only by high concentrations of penicillin might nevertheless be killed rapidly, while strains sensitive to minute concentrations might be killed only slowly. 4. Within the same bacterial suspension, individual organisms varied only to a minor degree with respect to the effective concentrations of penicillin. They varied strikingly, however, in their resistance to penicillin as measured by the times required to kill varying proportions of the cells.

New manufacturing process to develop antibacterial dyed polyethylene terephthalate sutures using plasma functionalization and chitosan immobilization

2021 ◽  
pp. 152808372110505
Author(s):  
Nesrine Bhouri ◽  
Faten Debbabi ◽  
Abderrahmen Merghni ◽  
Esther Rohleder ◽  
Boris Mahltig ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyethylene Terephthalate ◽  
Manufacturing Process ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
The United States ◽  
Mechanical Tests ◽  
Inflammatory Reactions ◽  
Plasma Functionalization

The main purpose of this paper is to develop a new manufacturing process leading to have antibacterial dyed non-absorbable braided polyethylene terephthalate (PET) sutures using biocompatible and non-toxic products. This manufacturing process allows better visibility of sutures in the surgical field and reduces the risk of infections and inflammatory reactions without affecting the mechanical properties while meeting the United States Pharmacopeia (USP) requirements. Plasma functionalization, acrylic acid (AA) grafting, and bioactive chitosan (CH) coating were used before the dyeing process with a biocompatible non-toxic acid dye, approved by the Food and Drug Administration (FDA). The influence of experimental parameters on the suture properties and the K/S values of the dyed sutures are investigated. Infrared spectroscopy confirms the presence of new bonds to immobilize chitosan on the surface of the suture. Mechanical tests confirm that the mechanical properties of sutures have not been affected. The in vitro antibacterial effect of dyed PET sutures showed an inhibition zone of 11 mm against S. aureus, 4 mm against P. aeruginosa, and 1 mm against E. coli. This study reveals that the new finishing process of sutures is a promising method to achieve an antibacterial effect with a uniform shade and smooth surfaces.

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