New manufacturing process to develop antibacterial dyed polyethylene terephthalate sutures using plasma functionalization and chitosan immobilization

2021 ◽  
pp. 152808372110505
Author(s):  
Nesrine Bhouri ◽  
Faten Debbabi ◽  
Abderrahmen Merghni ◽  
Esther Rohleder ◽  
Boris Mahltig ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Polyethylene Terephthalate ◽  
Manufacturing Process ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
The United States ◽  
Mechanical Tests ◽  
Inflammatory Reactions ◽  
Plasma Functionalization

The main purpose of this paper is to develop a new manufacturing process leading to have antibacterial dyed non-absorbable braided polyethylene terephthalate (PET) sutures using biocompatible and non-toxic products. This manufacturing process allows better visibility of sutures in the surgical field and reduces the risk of infections and inflammatory reactions without affecting the mechanical properties while meeting the United States Pharmacopeia (USP) requirements. Plasma functionalization, acrylic acid (AA) grafting, and bioactive chitosan (CH) coating were used before the dyeing process with a biocompatible non-toxic acid dye, approved by the Food and Drug Administration (FDA). The influence of experimental parameters on the suture properties and the K/S values of the dyed sutures are investigated. Infrared spectroscopy confirms the presence of new bonds to immobilize chitosan on the surface of the suture. Mechanical tests confirm that the mechanical properties of sutures have not been affected. The in vitro antibacterial effect of dyed PET sutures showed an inhibition zone of 11 mm against S. aureus, 4 mm against P. aeruginosa, and 1 mm against E. coli. This study reveals that the new finishing process of sutures is a promising method to achieve an antibacterial effect with a uniform shade and smooth surfaces.

scholarly journals Pengaruh Antibakteri Ekstrak Kulit Batang Matoa (Pometia pinnata) terhadap Bakteri Staphylococcus aureus secara In vitro

Jurnal MIPA ◽  
2013 ◽  
Vol 2 (2) ◽  
pp. 128 ◽  
Author(s):  
Mercy Ngajow ◽  
Jemmy Abidjulu ◽  
Vanda S. Kamu
Keyword(s):  
Staphylococcus Aureus ◽  
Secondary Metabolites ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Stem Bark ◽  
Positive Control ◽  
Negative Control ◽  
Bark Extract ◽  
Distilled Water

Telah dilakukan penelitian secara kualitatif terhadap pengaruh antibakteri dari ekstrak kulit batang matoa (Pometia pinnata. Spp.) terhadap bakteri Gram positif Staphylococcus aureus . Sebelum dilakukan uji antibakteri, sampel yang telah diekstrak secara maserasi diuji fitokimia terlebih dahulu untuk menentukan kandungan metabolit sekunder yang telah diketahui berperan sebagai agen antibakteri. Setelah dilakukan uji fitokimia, ekstrak diuji aktivitas antibakterinya terhadap bakteri Staphylococcus aureus dengan menggunakan teknik difusi agar dengan cara sumuran. Ekstrak dilarutkan pada aquades steril dengan perbandingan 2 g ekstrak pada 2 mL air. Untuk kontrol positif, digunakan ciprofloxacin dan aquades steril sebagai kontrol negatif. Hasil penelitian ini menunjukkan bahwa ekstrak kulit batang matoa memiliki aktivitas antibakteri setelah diinkubasi selama 24 jam pada 37 OC. Dari 3 kali pengulangan dengan masing – masing 3 sumuran, didapat zona hambat masing – masing 16.84 mm, 12.5 mm dan 14.5 mm dengan kontrol positif 29.67 mm serta kontrol negatif 0 mm. Hasil yang diperoleh didukung oleh keberadaan metabolit sekunder hasil uji fitokimia yaitu tanin, flavonoid, terpenoid dan saponin.A qualitative study has done  of the antibacterial effect of matoa (Pometia pinnata) stem bark extract against Gram-positive bacteria Staphylococcus aureus . Before the antibacterial test , samples were extracted by maceration and then  phytochemical  tested to measuring the content of secondary metabolites  that have been known to act as an antibacterial agent . After being tested of phytochemical , extracts were tested the antibacterial effect  against Staphylococcus aureus using agar diffusion technique. Extract was dissolved in sterile distilled water with a ratio of 2 g of extract in 2 mL of water . For the positive control , use of ciprofloxacin and sterile distilled water as a negative control . Results of this study indicate that matoa bark extract has antibacterial effect after incubation for 24 h at 37OC . Of 3 times with each repetition - each 3 wells, the inhibition zone obtained - each 16.84 mm , 12.5 mm and 14.5 mm with 29.67 mm of positive control and a negative control by 0 mm . The results are supported by the presence of secondary metabolites by phytochemical test such as tannins , flavonoids , terpenoids and saponins.

scholarly journals Antibacterial Effect Of Kaempferia Galanga L Extract On Lactobacillus Acidophilus –In Vitro

2017 ◽  
Vol 1 (01) ◽  
pp. 22
Author(s):  
Josi Saraswati ◽  
Annisa Septalita ◽  
Arini Bovita. N
Keyword(s):  
Antibacterial Activity ◽  
Lactobacillus Acidophilus ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Inhibition Effect ◽  
Kaempferia Galanga ◽  
Significant Difference ◽  
Agar Media ◽  
Negative Controls

Introduction: Lactobacillus acidophilus is one of the bacteria causes dental caries. The previous study has shown that Kaempferia galanga extract has a potential to inhibit the growth of Lactobacillus acidophilus.Objective: To determine the antibacterial effect of Kaempferia galanga extract to Lactobacillus acidophilus.Methods:Kaempferia galanga is extracted in 3 different solvents:dichlormethane, ethanol, and aquades. For each solvent, 0.2 μl Kaempferia galanga extractdroped into 6 mm steril paper dics. 0.1 ml Lactobacillus acidophilus inoculated on MRS agar. Each disc contains extract were impragnated into the agar media, then incubated at 370C for 24 hours, and inhibition zone measured.Results: Mean scores of Kaempferia galanga extract in 3 different solvents are: Kaempferia galanga (dichlormethane) is 1.6400; Kaempferia galanga (ethanol) is 1.7440; Kaempferia galanga extract is 1.6600; boiled Kaempferia galanga is 1.7000. Using Mann-Whitney Test, the results are: negative controls have no inhibition effect on Lactobacillus acidophilus compaired to Kaempferia galanga extract, comparation of those 4 Kaempferia galangal treatments shows no significant difference, those 4 Kaempferia galanga treatments compaired to erythromycin antibacterial effect shows significant difference, otherwise 4 Kaempferia galanga treatments compaired to penicillin shows no significant difference except Kaempferia galanga (ethanol).Conclusions: Kaempferia galanga extract can kill Lactobacillus acidophilus. Inhibition effect of Kaempferia galanga extract has no significant difference to penicillin but lower inhibition effect than erythromycin. The Kaempferia galanga extracts showed better antibacterial activity than penicillin.

scholarly journals Wild tembelek plant (Lantana camara) as a potential bioactive natural product againts Streptococcus pyogenes in Indonesia

2020 ◽  
Vol 4 (1) ◽  
pp. 77
Author(s):  
Ricky Indra Alfaray ◽  
Rafiqy Sa'adiy Faizun ◽  
Lionardy Yodianto ◽  
Saruuljavkhlan Batsaikhan ◽  
Yudith Annisa Ayu Rezkitha
Keyword(s):  
Streptococcus Pyogenes ◽  
Antibacterial Effect ◽  
Infectious Agent ◽  
Inhibition Zone ◽  
Significant Inhibition ◽  
Lantana Camara ◽  
Wild Plant ◽  
Control Group ◽  
Post Test

Abstract  Infectious diseases are common problems in most countries. Streptococcus pyogenes is the infectious agent that causes diseases such as pharyngitis, impetigo, toxic shock syndrome, and necrotizing fasciitis. Tembelek (Lantana camara) is a wild plant that can easily be found in every ecosystem in Indonesia whether in nature or settlements and known as a plant that has an antibacterial effect but the knowledge about its potential against Streptococcus pyogenes in this past five years remain scant. The aim of this study was to determine the potentiality of Lantana camara leaves and flowers extract against Streptococcus pyogenes. In this experimental study, in vitro using Post-test Only Control Group Designed, has been done and confirmed by the Indonesian Institute of Sciences. Lantana camara leaves and flowers extracts were obtained by maceration using ethanol. The extracts were diluted into eight concentrations and their antibacterial activity against Streptococcus pyogenes was tested using the Kirby-Bauer disc then proceeded Minimum Inhibitory Concentration (MIC) test and phytochemical assay. The data processed using SPSS software version 22. The results showed that flowers extract had the most significant inhibition zone (11.85 ± 0.119 mm) compared with the leaves extract (9.54 ± 0.07 mm) at the highest tested concentration was 640 mg/ml. The MIC of both extracts was 250 mg/ml. Flavonoids, phenolic, steroids, and saponins were found in both extracts whereas, alkaloid was found only in flowers extract. In conclusion, the Tembelek plant has an antibacterial effect against Streptococcus pyogenes. Future study is needed related to its mechanism of antibacterial effect. Keywords                : Antibacterial potential, Lantana camara; Streptococcus pyogenes

scholarly journals Evaluation of Antibacterial Activity of Essential Oils and Their Combination against Multidrug-Resistant Bacteria Isolated from Skin Ulcer

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Eshetu Gadisa ◽  
Hydar Usman
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Antibacterial Effect ◽  
Wound Care ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
Multidrug Resistant Bacteria

Background. Emerging of multidrug-resistant bacteria can compromise the effectiveness of antibiotics used to treat skin infections. Those bacteria imposed public health problems and questioning medical care in the 21st century. In this circumstance, essential oils of medicinal plants origin are supreme sources of structural and functionally divergent compounds, which inhibited the growth of common wound colonizing MRSA and ESBL producing P. aeruginosa. The aim of this study was to evaluate the combined antibacterial activity of essential oils extracted from Rumex abyssinicus, Cucumis pustulatus, and Discopodium penninervium against multidrug-resistant (MDR) isolates of skin ulcers. Methods. Essential oils (EOs) were extracted from aerial parts of R. abyssinicus, C. pustulatus, and D. penninervium with steam distillation. A mixture of each oil (1 : 1) was adsorbed to a disc and placed on Mueller Hinton Agar. Then, minimum zone of inhibition and bactericidal concentration of EOs was measured after incubeted for 18–24 hours at 37 °C. Their combined antibacterial effect was determined by the fractional inhibitory concentration index. Results. The antibacterial activity of mixed oil varied in their doses and bacteria species, of which a mixture of essential oil of R. abyssinicus and D. penninervium had inhibition zone (32 mm); its MIC and MBC values range from 1-2 μl/ml against MRSA. It had an inhibition zone (36 mm), MIC value 4 μl/ml, and MBC (8 μl/ml) against ESBL producing P. aeruginosa, whereas combined effects of R. abyssinicus and C. pustulatus had MIC values ranging from 2–8 μl/ml for E. coli and K. pneumoniae and 2 μl/ml for MRSA. There was a strong synergistic effect between R. abyssinicus and D. penninervium and promising antibacterial effect more specifically on MRSA and P. aeruginosa. Conclusion. This in vitro study of the combined effect of EOs has significant antibacterial activity on wound colonizing bacteria and reduces delaying wound healing as that of modern drugs tested in parallel. Hence, further structural elucidation of active compounds helps us to properly design or synthesis of topical antibiotics for wound care.

Mechanical Properties of Experimental Non-Latex Orthodontic Elastic Bands

2020 ◽  
Vol 897 ◽  
pp. 185-189
Author(s):  
Sasatorn Malanon ◽  
Surachai Dechkunakorn ◽  
Niwat Anuwongnukroh ◽  
Pongdhorn Sea-Oui ◽  
Puchong Thaptong ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Mechanical Tests ◽  
Lower Percentage ◽  
Internal Diameter ◽  
Cross Sectional ◽  
Force Decay ◽  
Extension Force

. Elastics, a source of continuous orthodontic force, are divided into two types, latex and non-latex, which are made from natural rubber and synthetic rubber, respectively. The major advantage of natural latex elastics is its resiliency to intraoral tractive forces. However, as the incidence of allergic reactions to natural latex has become more widely recognized, non-latex orthodontic elastics have been developed as an alternative. The aim of this study is to investigate the in vitro mechanical properties of Thai non-latex orthodontic elastics as compared to commercially available products. 30 samples of each two Thai non-latex elastics (MTEC1, MTEC2) and two commercial elastics (AO, GAC) with a specified diameter of ¼ inches were used. Width, cross-sectional thickness (CT), and internal diameter (ID) of all samples were measured. Mechanical tests were then carried out to determine the initial extension force (F0), 24-hour residual force (F24), and percentage of force decay. The data were analyzed with one-way ANOVA and Tukey’s test (p < 0.05). Statistically significant differences in elastic width among all four groups except between the Thai non-latex groups (MTEC1 and MTEC2) were found. AO elastics showed the greatest CT followed by GAC, MTEC2 and MTEC1. ID was significantly highest in GAC elastics and lowest in MTEC1 elastics. Although MTEC1 elastics had the lowest F0, the force still falls within the acceptable range for tooth movement (100-250g or 0.981–1.471N). MTEC2 elastics had the greatest F24 and also the lowest percentage of force decay followed by MTEC1, GAC, and AO elastics, which displayed the highest force decay, though no significant differences were found between the two commercial elastics. Thai non-latex elastics are suitable for orthodontic tooth movement due to its lower percentage of force decay after 24 hours.

scholarly journals Antibacterial and Cytotoxic Effects of Moringa oleifera (Moringa) and Azadirachta indica (Neem) Methanolic Extracts against Strains of Enterococcus faecalis

2018 ◽  
Vol 2018 ◽  
pp. 1-5 ◽  
Author(s):  
Lucía Arévalo-Híjar ◽  
Miguel Ángel Aguilar-Luis ◽  
Stefany Caballero-García ◽  
Néstor Gonzáles-Soto ◽  
Juana Del Valle-Mendoza
Keyword(s):  
Enterococcus Faecalis ◽  
Azadirachta Indica ◽  
Moringa Oleifera ◽  
Antibacterial Effect ◽  
Methanolic Extract ◽  
Inhibition Zone ◽  
Bactericidal Effect ◽  
Growth Inhibition Zone ◽  
Methanolic Extracts

Objective. To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods. The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results. The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions. In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.

Results of experimental studies into high-temperature adhesive compositions on bismaleimide base as applied to rocket and space hardware

2020 ◽  
pp. 24-34
Author(s):  
Andrey I ALYAMOVSKIY ◽  
Dmitry Ya. DAVYDOV ◽  
Elena V. ZEMTSOVA ◽  
Nikolay I. KOPYL
Keyword(s):  
Mechanical Properties ◽  
High Temperature ◽  
Manufacturing Process ◽  
Fracture Behavior ◽  
Experimental Studies ◽  
Mechanical Tests ◽  
Volatile Content ◽  
Degree Of Cure ◽  
Film Adhesive ◽  
Adhesive Compositions

The paper presents results of comprehensive experimental studies into high-temperature adhesive compositions and foaming paste on bismaleimide base, and characterizes their properties from the standpoint of the manufacturing process (thixotropy, foaming coefficient, volatile content, degree of cure, spreadability and fluidity) and mechanical strength (ultimate strength for shear, uniform tear and compression). The effect of accelerated environmental tests and irradiation on mechanical properties was studied and, and the level of offgassing in vacuum was determined for the adhesive compositions and the the foaming paste. Key words: high-temperature adhesive compositions, film adhesive, thixotropy, foaming coefficient, degree of cure, spreadability, fluidity, mechanical tests, fracture behavior, accelerated climatic tests, irradiation.

Influence of the Embedding Media on the Results of Standard In Vitro Fatigue Tests on the Femoral Components

1994 ◽  
Vol 208 (3) ◽  
pp. 131-137 ◽  
Author(s):  
M Viceconti ◽  
A Toni ◽  
A Giunti
Keyword(s):  
Mechanical Properties ◽  
Stress Analysis ◽  
Mechanical Tests ◽  
Fatigue Tests ◽  
Strain Gauges ◽  
Static Properties ◽  
Static Mechanical ◽  
Hip Stems ◽  
Dental Applications

The aim of the present investigation was to determine the effects of the embedding material mechanical properties on the in vitro fatigue endurance of femoral hip stems as determined following ISO standards. Acrylic cements are usually employed as embedding media; due to the high cost of surgical cements, acrylic cements for dental applications are commonly used. Two acrylic cements for dental applications were tested to determine their static mechanical properties and compared with those found for three surgical cements. The same two dental cements were also compared as embedding media in a series of ISO 7206/3 fatigue tests on a mini-size Cr-Co stem; prosthesis stress analysis using strain gauges was also performed. The mechanical tests on the different cements showed that behind the definition of acrylic cement are materials with considerably different mechanical static properties. The fatigue campaign confirmed the influence of embedding media on the results obtained from the ISO 7206/3 fatigue test. Under a load of 4200 N the stem failed after four million cycles when using one cement; it did not fail after ten million cycles when using another cement. Stress analysis with strain gauges substantially confirmed these findings.

scholarly journals Thermoplastic Polymers with Nanosilver Addition—Microstructural, Surface and Mechanical Evaluation during a 36-Month Deionized Water Incubation Period

Materials ◽  
2021 ◽  
Vol 14 (2) ◽  
pp. 361
Author(s):  
Magdalena Ziąbka ◽  
Michał Dziadek
Keyword(s):  
Mechanical Properties ◽  
Contact Angle ◽  
Silver Nanoparticles ◽  
Incubation Period ◽  
Water Environment ◽  
Mechanical Tests ◽  
Deionized Water ◽  
Thermoplastic Polymers

Three types of thermoplastic polymers, acrylonitrile butadiene styrene (ABS), polymethyl methacrylate acrylic (PMMA) and high-density polyethylene (HDPE), were enriched with silver nanoparticles (AgNPs) of 0.5 wt.% and 1.0 wt.%, respectively. The polymers and the composites were manufactured via injection molding. Regarding the potential of these polymers as matrices for long-term use as biomaterials, the aim of this study was to examine their stability in the in vitro conditions during a three-year incubation period in deionized water. In this work, microstructural observations were performed, and mechanical properties were assessed. Surface parameters, such as roughness and contact angle, were comprehensively investigated. The microstructural evaluation showed that the silver additive was homogeneously dispersed in all the examined matrices. The 36-month immersion period indicated no microstructural changes and proved the composites’ stability. The mechanical tests confirmed that the composites retained comparable mechanical properties after the silver incorporation. The Young’s modulus and tensile strength increased during long-term incubation. The addition of silver nanoparticles did not alter the composites’ roughness. The contact angle increased with the rising AgNP content. It was also shown that the materials’ roughness increased with the incubation time, especially for the ABS- and HDPE-based materials. The water environment conditions improved the wettability of the tested materials. However, the silver nanoparticles’ content resulted in the contact angle decreasing during incubation. The conducted studies confirmed that the mechanical properties of all the polymers and composites did not deteriorate; thus, the materials may be considered stable and applicable for long-term working periods in aqueous environments.

scholarly journals In vitro Evaluation of Antibacterial Activity and Protective Effects on Cardiomyocytes with H/R Injury of Garlic/Onion Oil

2020 ◽  
Author(s):  
Yifei Lu ◽  
Hui Lv ◽  
Lihua Li ◽  
Chao Yang ◽  
Da Pan ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Survival Rate ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Protective Effects ◽  
Volatile Oils ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Garlic Oil

Abstract Background: At present, the research concerning in vitro experiments of garlic oil and onion oil is limited. The objective of this study was to carry out the research on the effective components of garlic and onion, and to study the antibacterial activity of the two essential oils and their protective effects on cardiomyocytes with hypoxia/reoxygenation (H/R) injury.Method: The protective effect of volatile oils at different concentrations (200, 100, 50, 25, 12.5, 6.3, 3.1mg/L) on the endothelial cell of microvascular in cardiac muscle against H/R injury was examined by MTT and flow cytometer method. The survival rate and apoptosis situation were record. Inhibition zone test was used to evaluate antibacterial activity of volatile oils. MIC and MBC were calculated as well.Results: To achieve a higher survival rate, the optimum concentrations of garlic oil and onion oil were 12.5mg/L and 6.13mg/L and high concentrations of garlic and onion oil decreased the survival rate. Both the 12.5mg/L onion and garlic oil reduced the apoptosis situation of cardiomyocytes. Both garlic oil and onion oil had antibacterial effect on gram-positive bacteria and gram-negative bacteria, and had the best antibacterial effect on cocci of gram-positive bacteria.Conclusion: Garlic and onion oil do have antibacterial activity and protective effects on cardiomyocytes with H/R injury in a concentration range.

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