scholarly journals EKSPRESI Nuclear Factor of Activated T cells c-1 (NFATc-1) DAN OSTEOKALSIN PADA KERUSAKAN TULANG PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis

2019 ◽  
Vol 7 (2) ◽  
pp. 138
Author(s):  
Arindah Hadi ◽  
M. Roelianto ◽  
Ari Subiyanto ◽  
Tamara Yuanita
Keyword(s):  
Treatment Failure ◽  
Bone Destruction ◽  
Lipoteichoic Acid ◽  
Endodontic Treatment ◽  
Male Rats ◽  
Control Group ◽  
Activated T Cells ◽  
Control Group Design ◽  
Group A ◽  
Group B

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation then lead to bone destruction. Bone destruction occurs due to the inflammation process that is mediated by immune system. The important cell in the process of bone destruction is osteoclast. Bone destruction is marked by the form of osteoclast that is called osteoclastogenesis. NFATc-1 and osteocalcin play important things in osteoclastogenesis. Purpose. The aim of this study is to know about the expression of NFATc-1 and osteocalcin during the periapical bone destruction due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of periapical bone, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of NFATc-1 and osteoclast increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of NFATc-1 and osteocalcin are increasing during the periapical bone destruction that induced by E.faecalis.

scholarly journals EKSPRESI MATRIKS METALLOPROTEINASE-8 DAN INTERLEUKIN-8 PADA KERUSAKAN JARINGAN PERIAPIKAL AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS

2019 ◽  
Vol 7 (2) ◽  
pp. 95
Author(s):  
Tamara Yuanita ◽  
Tantri Wismayaning Radito ◽  
Dian Agustin Wahjuningrum ◽  
R. Roulianto
Keyword(s):  
Treatment Failure ◽  
Enterococcus Faecalis ◽  
Tissue Damage ◽  
Endodontic Treatment ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Collagen Formation ◽  
Group A ◽  
Group B

Background. The main etiology of endodontic treatment failure  is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-8 to enhance the inflamation reaction and MMP-8 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of MMP-8 and IL-8 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry  followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-8 and IL-8 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of MMP-8 and IL-8 are increasing during the periapical tissue damage that induced by E.faecalis.

scholarly journals EKSPRESI TGF-B DAN MMP-1 PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS TIKUS WISTAR

2019 ◽  
Vol 7 (2) ◽  
pp. 86
Author(s):  
Tamara Yuanita ◽  
Hadriany Hotmaria ◽  
Ruslan Effendy ◽  
Ketut Suardita
Keyword(s):  
Treatment Failure ◽  
Enterococcus Faecalis ◽  
Tissue Damage ◽  
Negative Control ◽  
Endodontic Treatment ◽  
Male Rats ◽  
Control Group ◽  
Control Group Design ◽  
Collagen Formation ◽  
Group B

Background. The main etiology of endodontic treatment failure  is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of TGF-ß to enhance the proliferation collagen and MMP-1 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of of TGF-ß and MMP-1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 27 male rats were randomly divided into 3 main groups. Group A (negative control) : every tooth was’nt induced by anything. Group B ( positive control): every tooth was induced only by sterile BHIb and closed by GIC Fuji II as the final restoration. Group C (: every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), and closed by GIC Fuji II as the final restoration. The animals were sacrificed after 21 days and prepared for histological examination of tissue damage, then we did the immunohistochemistry  followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-1 increased significantly in group C when E.faecalis was induced. When expression of TGF-ß decreaced significantly in group C rather than group B.  Conclusion. From this study we know that the expression of TGF-ß and MMP-1 are make opposite pathway due to chronic apical periodontitis that induced by E.faecalis.

scholarly journals EKSPRESI Interleukin-1 (IL-1) PADA PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis

2019 ◽  
Vol 7 (2) ◽  
pp. 59
Author(s):  
Yuliana Dwiwahyu Suryandari ◽  
Ketut Suardita ◽  
M. Mudjiono ◽  
Tamara Yuanita
Keyword(s):  
Tissue Damage ◽  
Root Canal ◽  
Interleukin 1 ◽  
Male Rats ◽  
Control Group ◽  
Main Role ◽  
Control Group Design ◽  
Collagen Formation ◽  
Group A ◽  
Group B

Background. Root canal treatment is a main role in decreasing infection from root canal and pulp. The main cause of periapical damage mostly are bacteries. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-1. IL-1 is the proinflammation cytokine that is the key of host response bacteria invation and tissue damage. Also IL-1 could cause some indirectly tissue damage through the activation of MMPS. MMPs to stop the collagen formation. Purpose. The aim of this study is to know about the expression of IL- 1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of IL-1 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of IL-1 is increasing during the periapical tissue damage that induced by E.faecalis.

scholarly journals EFFECT OF CHEWING MARSHMALLOW CONTAINING BETEL CHEW IN REDUCING STREPTOCOCCUS MUTANS AND PLAQUE INDEX ON CHILDREN

2019 ◽  
pp. 83-86
Author(s):  
RINDIT PAMBAYUN ◽  
ADE PUTRI ◽  
MEIDY TRI YUDA ◽  
SITI RUSDIANA PUSPA DEWI ◽  
TRI WARDANI WIDOWATI ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Plaque Index ◽  
Chemistry Laboratory ◽  
Control Group ◽  
Control Group Design ◽  
Islamic School ◽  
Post Test ◽  
Quasi Experimental ◽  
Group A ◽  
Group B

Objective: Marshmallow is a soft candy loved by all levels of society, especially children. Sucrose contained in marshmallow increases the growth of Streptococcus mutans and plaque. Betel chew as Indonesian traditional plants add in marshmallow was believed to decrease S. mutans and plaque. The aim of this study was to evaluate the effect of chewing marshmallow containing betel chew in reducing S. mutans and plaque index in children. Methods: This research was a quasi-experimental with pre-test and post-test control group design. It was conducted in the Laboratory of Agricultural Chemistry, Laboratory of Agricultural Technology Department, and Islamic School of Al-Amalul Khair. Thirty students were divided into two groups. Group A was chewing marshmallow without betel chew and Group B was chewing marshmallow containing betel chew. Results: The results showed that chewing marshmallow without betel chew increased the growth of S. mutans and plaque index significantly. Chewing marshmallow without betel chew inhibited the growth of S. mutans and dental plaque formation. Conclusion: Chewing marshmallow containing betel chew reduces S. mutans and plaque index on children.

scholarly journals Efek Seduhan Kopi Robusta terhadap Jumlah Limfosit pada Daerah Tarikan Ligamen Periodontal Gigi Marmut (Cavia Cobaya) yang Diinduksi Gaya Mekanis Ortodonti

2020 ◽  
Vol 7 (3) ◽  
pp. 182
Author(s):  
Ginanjar Hidayatullah ◽  
Herniyati Herniyati ◽  
Leliana Sandra Deviade Putri
Keyword(s):  
Periodontal Ligament ◽  
Alveolar Bone ◽  
Control Group ◽  
Control Group Design ◽  
Robusta Coffee ◽  
Coffee Brew ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Coffea Robusta

Malocclusion is an occlusion that deviates from the normal state, so it needs to be treated using an orthodontic device. The formation of new alveolar bone after obtaining an orthodontic force will be surrounded by osteoid bones are not stable enough (relapse). Robusta coffee (Coffea robusta) has flavonoids which can increase lymphocyte proliferation. The purpose of this study was to analyze the effect of robusta coffee on the number of lymphocyte cells in the tension area of marmot’s (Cavia cobaya) periodontal ligament which induced by mechanical orthodontic forces. This type of research is laboratory experimental with the Post Test Only Control Group Design. The average number of lymphocyte cells in group A = 0.75, B = 1.45, C = 1.26, D = 1.54. One-way Anova test results showed that the number of lymphocyte cells in all groups showed a significant difference (p <0.05). The results of the post hoc LSD test showed that group A had significant differences with group B and group D (p <0.05). While group C did not have a significant difference with group A (p> 0.05). The conclusion obtained is that the provision of robusta coffee brew for 2 weeks can increase the number of lymphocyte cells in the tension area of marmot’s (Cavia cobaya) periodontal ligament which induced by orthodontic mechanical forces compared to without robusta coffee brew.

scholarly journals PENGARUH DOSIS EKSTRAK JAMBU BIJI (PSIDIUM GUAJAVA. L) DAN TABLET Fe TERHADAP PERUBAHAN KADAR HEMOGLOBIN PADA MENCIT (MUS MUSCULUS)

2018 ◽  
Vol 10 (3) ◽  
pp. 102-107
Author(s):  
Yusnaini Yusnaini
Keyword(s):  
Mus Musculus ◽  
Experimental Studies ◽  
Psidium Guajava ◽  
Control Group ◽  
Control Group Design ◽  
Laboratory Design ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Post Hoc

According to the WHO 40% of maternal deaths in developing countries related to anemia in pregnancy and is most commonly caused by iron deficiency and acute bleeding, even less so the two interact. This study aims to determine the effect of doses of extracts of Guava (Psidium Guajava. L) and Tablet Fe to changes in hemoglobin levels in mice (Mus musculus). Type True Experimental studies in laboratory design nonrandomized design Pretest-Posttest Control Group Design. Samples were mice (Mus musculus) as many as 20 birds were divided into four groups with each group number as many as five tails. Group A was given a tablet Fe as much as 0.126 mg / kg / day, group B was given a tablet Fe as much as 0.126 mg / KgBW / day coupled with extracts of Guava as much as 10 mg / kg / day, group C was given tablets Fe as much as 0.126 mg / kg / day coupled with guava extract as much as 20 mg / kg / day, group D was given tablets as much as 0.126 mg Fe / KgBW / day plus guava extract as much as 30 mg / kg / day. The data collection was conducted from June 5 s / d July 20, 2015. Data were analyzed using Paired T-Test continued with Test and One Way ANOVA Post Hoc Test namely LSD test. Results showed that there was no significant difference in hemoglobin levels between the groups in which the value of P = 0634> 0.05.. Based on the results of this study concluded that there was no effect of dose of extract of guava (Psidium Guajava. L) and Tablet Fe to changes in hemoglobin levels in mice (Mus musculus), but the effect on the number of erythrocytes of mice (Mus musculus)

scholarly journals The Hepatotoxic and Nephrotoxic Effects of Purwoceng (Pimpinella pruatjan Molk.) Roots Ethanol Extract Administration in Subchronic Dose

Molekul ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. 163
Author(s):  
Fitranto Arjadi ◽  
Nur Signa Aini Gumilas ◽  
Ika Murti Harini ◽  
Vitasari Indriani ◽  
Lantip Rujito
Keyword(s):  
Dose Level ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Control Group ◽  
Serum Glutamic Pyruvic Transaminase ◽  
Control Group Design ◽  
Wistar Strain ◽  
Post Test ◽  
Group A ◽  
Group B

Purwoceng (Pimpinella pruatjan Molk) is an original Indonesian herb which is known to have an aphrodisiac effect. The active compounds in Purwoceng potentially have hepatotoxic and nephrotoxic effects.This study was aimed to analyze the effect of subchronic administration of Purwoceng roots ethanol extract to Wistar Strain of Rattus norvegicus rats. The method of this subchronic toxicity study was an experimental post test only with control group design. Forty male Rattus norvegicus were randomly divided into four groups and get 28-days treatment. Group A as control received aquadest and 1% CMC (carboxymethyl cellulose), group B, C, and D were given Purwoceng roots ethanol extract of 42, 84, and 168 mg/KgBW/day and 1% CMC. Parameters tested were the levels of urea, creatinine, SGPT (serum glutamic pyruvic transaminase), SGOT(serum oxaloacetic pyruvic transaminase), kidney and hepar histopatology.The results showed a statistically significant for the liver histopathological in group B, creatinine, urea, and kidney histopathology  in group C. Our study concluded that subchronic administration of Purwoceng (Pimpinella pruatjan Molk) roots ethanol extract could induce hepatotoxicity at the 42 mg/KgBW/day dose level and nephrotoxicity at the 84 mg/KgBW/day dose level.

scholarly journals EFEK MIE PISANG DAN SUKUN TERHADAP GLUKOSA DARAH SEWAKTU PADA RESPONDEN BUKAN PENDERITA DIABETES MELLITUS (The Effect of Banana and Breadfruits Noodles on Glucose Levels of Non Diabetic Mellitus Respondents)

Jurnal NERS ◽  
2016 ◽  
Vol 11 (2) ◽  
pp. 246
Author(s):  
Nursalam Nursalam ◽  
Rista Fauziningtyas ◽  
Candra Panji Asmoro ◽  
Kusnanto Kusnanto ◽  
Merryana Adriani
Keyword(s):  
Public Awareness ◽  
Control Group ◽  
Diabetic Mellitus ◽  
Control Group Design ◽  
Glucose Levels ◽  
Group A ◽  
The One ◽  
Group B ◽  
One Way Anova ◽  
Selection Of

ABSTRAK :Pendahuluan: Kesadaran masyarakat terhadap gaya hidup sehat menyebabkan perubahan pada pemilihan bahan makanan untuk dikonsumsi. Penambahan tepung Pisang dan Sukun meningkatkan kadar serat pada mie. Tujuan penelitian ini adalah untuk menentukan komposisi tepung pengganti pada mie yang berefek pada peningkatan GDS paling rendah. Metode: Desain penelitian ini adalah control group pretest posttest design.  Sample didapatkan sebanyak 58 orang mahasiswa Fakultas Keperawatan Unair yang memenuhi kriteria inklusi dan eksklusi. 58 sampel dibagi menjadi 3 kelompok, yaitu mi A (kontrol), B (subtitusi 30%) dan C (subtitusi 20%). Pemeriksaan GDS dilakukan sebelum pemberian Mi dan 60 menit setelah pemberian mi. Analisa data yang digunakan adalah dengan ANOVA one way. Hasil: Berdasarkan uji ANOVA one way didapatkan p=0,000 (p<0,050) yang berarti ada perbedaan hasil pada ke tiga kelompok tersebut. Kenaikan tertinggi Nilai GDS adalah pada kelompok mi A dan terendah kelompok mi B.Diskusi: Penambahan tepung pisang dan sukun pada mie dapat menurunkan kenaikan nilai GDS responden bukan penderita DM. Penelitian lebih lanjut perlu dilakukan untuk mengetahui kandungan mi dengan tepung pisang dan sukun dan respon GDS pada penderita DM. Kata Kunci: Mi, Tepung, Pisang, Sukun, Gula Darah, Diabetes ABSTRACT :Introduction: Public awareness of healthy lifestyles cause a change in the selection of foodstuffs for consumption. Bananas and breadfruit flour additions increase the fiber content on the noodles. The purpose of this study was to determine the composition of noodles flour substitute on the effect on an increase in the lowest Glucose levels. Methods: The research design was pretest posttest control group design. Sample obtained as many as 58 students of the Faculty of Nursing University of Airlangga who meet the inclusion and exclusion criteria. 58 samples were divided into three groups, namely Noodles A (control), B (substitution 30%) and C (substitution 20%). Glucose levels examination carried out before eating noodles and 60 minutes after eat it. The data analysis of this study was the one-way ANOVA. Results: Based on the one-way ANOVA test was obtained p = 0.000 (p <0.050), which means no differences in outcomes in all three groups. The highest increasing of glucose levels is group A and the lowest in group B. Discussion: The addition of banana and breadfruit flour on the noodles can reduce the increase in the glucose levels after eat it. Further research needs to be done to determine the content of noodles with a banana and breadfruit flour and glucose’s responses in patients with diabetic mellitus. Keywords : Noodles, Flour, Banana, Breadfruit,Glucose, Diabetic Mellitus.

scholarly journals Effect of Neem (Azadirachta Indica) on Fertility in Male Rats

2013 ◽  
Vol 5 (1) ◽  
pp. 39-42 ◽  
Author(s):  
Asma Khan ◽  
Zinnat Ara Begum ◽  
Md Ismail Khan ◽  
Hasanur Rahman ◽  
Layla Afroza Banu
Keyword(s):  
Azadirachta Indica ◽  
Leydig Cells ◽  
Structural Changes ◽  
Histological Study ◽  
Male Rats ◽  
Control Group ◽  
Medical College ◽  
Leaf Powder ◽  
Group A ◽  
Group B

Background: Azadirachta indica locally known as neem has been claimed to possess antifertility properties by many investigators. Objective: The present study was designed to evaluate the antifertility effect of Azadirachta indica leaf powder in male rats. Methodology: The study was carried out in pharmacology and Therapeutic Department of Dhaka Medical College during the period of January 2008 to December 2008. Total number of 24 rats was divided into 4 groups. Group A was served as control, group B, C and D were treated by Azadirachta indica leaf powder orally, inj. testosterone I/M, & Azadirachta indica leaf powder orally, inj. testosterone I/M respectively for 48 days. They were sacrificed on 49 day. Then morphological and histological study was done. Result: The result showed that the all of the Azadirachta indica leaf powder treated rats has significant antispermatognic effect as well as antiandrogenic effect. Conclusion: The Azadirachta indica leaf powder reduced motility and density of sperm and causes structural changes of leydig cells and seminiferous tubule. DOI: http://dx.doi.org/10.3329/jssmc.v5i1.16206 J Shaheed Suhrawardy Med Coll, 2013;5(1):39-42

scholarly journals HYPERPLASTIC THYROID GLAND

2010 ◽  
Vol 17 (01) ◽  
pp. 21-30
Author(s):  
ANWAAR HUSSAIN ◽  
Nabeela Kaukab ◽  
SAIF-U- DIN ◽  
Muhammad Ismail Ismail ◽  
KHALID MEHMOOD AKHTAR
Keyword(s):  
Thyroid Gland ◽  
Thyroid Diseases ◽  
Male Rats ◽  
Medical Institute ◽  
Control Group ◽  
Albino Rats ◽  
Follicular Cells ◽  
Thiourea Group ◽  
Group A ◽  
Group B

Aim and Objective: The study was conducted to see the effects of dexamethasone (synthetic corticosteroid) on involution ofhyperplastic thyroid gland in albino rats, and to evaluate its uses in thyroid diseases. Study Design: Comparative histological study done inPostgraduate Medical Institute Lahore in 1998. Materials and methods: 54 adult male rats were taken and divided at random into control grouphaving 27 rats and experimental groups containing 27 rats. Control group was given normal diet along with 2 microgram of Potassium iodidefor 21 days while experimental groups further subdivided into group A having 3 rats and group B and C containing 12 rats each. All theexperimental groups were treated with Thiourea. Group A was sacrificed on day 22 after withdrawal of thiourea. Group B was given Potassiumiodide after stoppage of thiourea on day 21 and were sacrificed on days 22, 26, 30 and 50 in 4 sub groups (B1- B4). Group C was injectedDexamethasone daily from day 22 to day 50 and sacrificed on same days in 4 subgroups (C1-C2) to study involution process. Results:Histologically experimental group A exhibited significant increase in width and length of follicular cells lining the small sized follicles havingscanty colloid. The results of experimental subgroups B revealed early and complete involution whereas subgroups C showed significantlypersistent hyperplastic changes in the form of tall follicular cells lining small empty follicles. Conclusion: Thus it was concluded thatdexamehasone did retain hyperplastic changes during involution process, so steroid should consciously be used in thyroid diseases.

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