scholarly journals Oxygen Hyperbaric Exposure Induces GLUT4 Expression Reduction and No Folliculogenesis Alterations in Rat PCOS with Insulin Resistance Model

2015 ◽  
Vol 23 (3) ◽  
pp. 112
Author(s):  
Varianida Veterini ◽  
Budi Santoso ◽  
Widjiati Widjiati
Keyword(s):  
Treatment Group ◽  
Hyperbaric Oxygen ◽  
Alternative Therapy ◽  
Placebo Treatment ◽  
Positive Control ◽  
Negative Control ◽  
Placebo Treatment Group ◽  
Glut4 Expression ◽  
Model Animal ◽  
Hyperbaric Exposure

 Objectives: To know effect of hyperbaric oxygen therapy to GLUT4 expression and folliculogenesis in rat PCOS with insulin resistent model.Materials and Methods: this is an analytic experimental study used 30 rats induced by testosterone propionate injection 1 mg/100 g body weight daily for 28 consecutive days as a PCOS with insulin resistent model. Animal models divided into 3 groups. Negative control was not given treatment, positive control was given placebo. Treatment group was given hyperbaric oxygen 2.4 ATA 90 minutes 2x5 days. GLUT4 expression determined by immunohystochemistry to m. Gastrocnemius. Hematoxylin Eosin staining to rat ovaries were performed to know differentiation in folliculogenesis. The results were then compared.Results: There were significant decrease of GLUT4 expression in treatment group (mean0,84+0,47)compared to negative and positive control (mean3,96+3,16and3,36+2,17). There were no different folliculogenesis in these groups.Conclusion: Hyperbaric oxygen 2.4 ATA 90 minutes 2x5 days induce decrease of GLUT4 expression and no alterations in folliculogenesis in rat PCOS with insulin resistent model, therefore can not yet be used as alternative therapy in PCOS.  

The Comparison of Ostecyte in the Pressure area and Tension area on Tooth Movement Because of Hyperbaric Oxygen Therapy

DENTA ◽  
2018 ◽  
Vol 12 (1) ◽  
pp. 34
Author(s):  
Arya Barahmanta ◽  
Muhammad Faizal Winaris ◽  
Pambudi Raharjo
Keyword(s):  
Hyperbaric Oxygen ◽  
Oxygen Therapy ◽  
Tooth Movement ◽  
Bone Remodelling ◽  
Orthodontic Tooth Movement ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Control Group ◽  
Pressure Area

<p><strong><em>Background:</em></strong><em> Orthodontic tooth movement is a </em><em>interaction prosess</em><em> of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO).  <strong>Purpose: </strong>To determine the difference number  of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. <strong>Materials and Methods</strong>: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male)  were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. <strong>Result:</strong> The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than  negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest  than positive control group (K+=3,83) and treatment (P=3,25). <strong>Conclusion: </strong>Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling.</em></p><pre><strong> </strong></pre><p><strong><em>Keywords:</em></strong><em> Hyperbaric Oxygen, Tooth movement, Bone remodeling, </em><em>Osteocyte</em><em></em></p><p><em> </em></p><p><strong><em>Correspondence:</em></strong><em> </em><em>Arya Brahmanta</em><em>, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email:</em><em> </em><a href="mailto:[email protected]"><em>arya.brahmanta</em><em>@</em><em>hangtuah.ac.id</em></a></p>

UJI IMUNOMODULATOR DARI EKSTRAK ETANOL DAUN WARU (HIBISCUS TILIACEUS) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT

2019 ◽  
Vol 1 (2) ◽  
pp. 11-16
Author(s):  
Yanna Rotua Sihombing ◽  
Debi Dinha Sitepu
Keyword(s):  
Treatment Group ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Delayed Type Hypersensitivity ◽  
Hypersensitivity Response ◽  
Delayed Type Hypersensitivity Response

Immunomodulator is a compound that can increaase the imuno system. One of the plants that have immunomodulator’s activity is Waru Leaf (Hibiscus tiliaceus). the purpose of this research was to test the effect of immunomodulator by extract of Waru Leaf ethanol on rat male. The activity of immunomodulator was determined by using digital pletysmometer by measuring the differences between the last leg swelling’s volume and the first leg swelling’s volume. The treatment group were divided into 5 groups. Each group consistof 5 rats CMC-Na 0,5% (negative control), Stimuno®  32,5 mg/kgBW (positive control), dose of EEDW 50, 100 and 200 mg/kgBW, and bacteria E.coli as antigen. The results slowed that distribution of EEDW dose 200 mg/kgBW can give the effect of immunostimulant by swelling enthancement compared by CMC-Na 0,5 %. EEDW 200 mg/kgBW that have activity comparable with Stimuno®  32,5 mg/kgBW. Thus, it is concluded that of Waru Leaf extract has immunomodulator effects on delayed-type hypersensitivity response of rat male.

scholarly journals Efektivitas Ekstrak Artemisia vulgaris L. sebagai Hepatoprotektor pada sel-sel Hati Tikus yang Diinduksi Niasin

2018 ◽  
Vol 6 (2) ◽  
pp. 251
Author(s):  
Laily Rahmawati ◽  
Erma Sulistyaningsih ◽  
Rosita Dewi
Keyword(s):  
Treatment Group ◽  
Liver Damage ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Artemisia Vulgaris ◽  
Group I ◽  
Group Ii

  The niacin in energy drinks has metabolic product that cause oxidative stress and liver damage, while the liver damage can be prevented by hepatoprotective agents. Scoparone in Artemisia vulgaris L. can act as a hepatoprotector by its antioxidant effect. This study aimed to investigate the effectivity of Artemisia vulgaris L. extract as a hepatoprotector in wistar hepatocytes induced by niacin. This study used 25 male rats which were divided into 5 groups: normal, the negative control, the positive control, the treatment group I, and II. Treatment was conducted for 28 days. The samples were terminated and the hepatocyte were prepared for histological examination. Histological appearance was catagorized as mild, moderate, and severe damage with or without inflamatory cells activity. The data analysis by Kruskal Wallis showed significant difference (p<0,001). Further analysis by Mann Whitney revealed significantly difference (p<0,05) between normal group and all groups, negative control group and positive control group, and positive control group and treatment group I, but not significantly difference between negative control group and treatment group I, negative control group and treatment group II, positive control group and treatment group II, and between treatment groups. The study concluded that the effectivity of Artemisia vulgaris L. extract has not been proven as a hepatoprotector but further study is needed to draw a definite conclusion.   Keywords: energy drink, niacin, Artemisia vulgaris L., hepatoprotector  

scholarly journals PENGGUNAAN KRIM EKSTRAK BATANG DAN DAUN SURUHAN (Peperomia pellucida L.H.B.K) DALAM PROSES PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH (Rattus norvegicus)

2015 ◽  
Vol 1 (2) ◽  
pp. 198-208
Author(s):  
Nur Fitri
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Healing Process ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Herbaceous Plant

Background: Peperomia pellucida L'HBK or known as messengers in the Indonesian plant is a herbaceous plant that belongs to the family Piperaceae. This study aimed to determine the effect of the stem and leaf extract cream messengers to the healing process of burns in rats (Rattus norvegicus. Methods: This was an experimental study using a completely randomized design. Test animals were divided into three groups, each - each group consisted of 3 rats. The first group is the negative control group (distilled water), the second group is a positive control group (Bioplacenton®), the third group is the group treated stem and leaf extract cream errand. The diameter of the wound and fibroblasts observed histopathology and is used as an indicator of the healing process of burns. The burns were treated and observed the healing effect for 20 days. Data were analyzed statistically wound diameter using ANOVA followed by LSD test. Results: The results showed the cream extracts of stems and leaves telling effect on the healing process of burns on rats. Conclusion: The results also showed that the treatment group and the leaf stem extract cream messengers and control groups positively influence the healing process of burns significantly when compared to the negative control group. Meanwhile, the treatment group stem and leaf extract cream messengers have no preformance difference influence the healing process of burns a significant positive control group

scholarly journals Potensi Ekstrak Daun Kelor (Moringa Oleifera) sebagai Hepatoprotektor pada Tikus Putih (Rattus novergicus) yang Diinduksi Parasetamol Dosis toksik

2018 ◽  
Vol 5 (1) ◽  
pp. 58
Author(s):  
Noer Kumala Indahsari ◽  
Masfufatun Masfufatun ◽  
Emilia Devi D.R
Keyword(s):  
Treatment Group ◽  
Moringa Oleifera ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Control Group ◽  
High Dose ◽  
Control Group Design ◽  
Post Test

Moringa Oleifera is a plant that contains chemical compounds that are useful, such as flavonoids. The ability of this flavonoid compound that can capture free radicals cause damage and hepatoprotektan hepar. Purpose of study was to determined levels of Moringa leaf extract which can overcome the effects of liver damage caused by toxic doses of paracetamol through MDA, SGOT and SGPT Method used in this laboratory experimental study is a Randomized Post Test Only Control Group Design with the following stages: 1. Moringa Leaf Extraction with Ethanol 96%; Try 2.Preparasi animals, 3. Treatment of Animals Try the extract of leaves of Moringa 3 dose is: 250mg / 200BB rat (dose of A), 500mg / 200BB mice (dose B), 1000mg / 200BB mice (dose C) for 14 days in combination with paracetamol 2 g / 200BB mice, compared to the negative control group (group given just paracetamol 2 g / 200BB rat) and the positive control group (the group who were given regular feed) for 14 days.Results : turned out to be no difference in the reduction in SGOT levels are statistically significant between the negative control group with high-dose treatment group ie the dose C with =0,016 smaller than 0.05, whereas a decrease in ALT levels were significantly decreased in the treatment group high dose is the dose C with =0,009 smaller than 0.05. While MDA group treated with the negative control group experienced an overall decline for the dose A with =0,05, dose B with =0,0011 and dose C with =0,001. Conclusion of this study showed that the extract of Moringa leaves can be potentially as an antioxidant in all doses at once can be as hepatoprotektor at high doses is 1000mg / 200BB Rattus Novergicus.

scholarly journals Phase II, Randomized, Double-Blind, Placebo-Controlled Studies of Ruprintrivir Nasal Spray 2-Percent Suspension for Prevention and Treatment of Experimentally Induced Rhinovirus Colds in Healthy Volunteers

2003 ◽  
Vol 47 (12) ◽  
pp. 3907-3916 ◽  
Author(s):  
Frederick G. Hayden ◽  
Ronald B. Turner ◽  
Jack M. Gwaltney ◽  
Kathy Chi-Burris ◽  
Merril Gersten ◽  
...  
Keyword(s):  
Treatment Group ◽  
Healthy Volunteers ◽  
Group Versus ◽  
Placebo Treatment ◽  
Pharmacokinetic Analysis ◽  
Nasal Discharge ◽  
Double Blind ◽  
Placebo Treatment Group ◽  
Double Blind Placebo ◽  
Viral Titers

ABSTRACT Human rhinovirus (HRV) infections are usually self-limited but may be associated with serious consequences, particularly in those with asthma and chronic respiratory disease. Effective antiviral agents are needed for preventing and treating HRV illnesses. Ruprintrivir (Agouron Pharmaceuticals, Inc., San Diego, Calif.) selectively inhibits HRV 3C protease and shows potent, broad-spectrum anti-HRV activity in vitro. We conducted three double-blind, placebo-controlled clinical trials in 202 healthy volunteers to assess the activity of ruprintrivir in experimental HRV infection. Subjects were randomized to receive intranasal ruprintrivir (8 mg) or placebo sprays as prophylaxis (two or five times daily [2×/day or 5×/day] for 5 days) starting 6 h before infection or as treatment (5×/day for 4 days) starting 24 h after infection. Ruprintrivir prophylaxis reduced the proportion of subjects with positive viral cultures (for 5×/day dosing groups, 44% for ruprintrivir treatment group versus 70% for placebo treatment group [P = 0.03]; for 2×/day dosing groups, 60% for ruprintrivir group versus 92% for placebo group [P = 0.004]) and viral titers but did not decrease the frequency of colds. Ruprintrivir treatment reduced the mean total daily symptom score (2.2 for ruprintrivir treatment group and 3.3 for the placebo treatment group [P = 0.014]) by 33%. Secondary endpoints, including viral titers, individual symptom scores, and nasal discharge weights, were also reduced by ruprintrivir treatment. Overall, ruprintrivir was well tolerated; blood-tinged mucus and nasal passage irritation were the most common adverse effects reported. Pharmacokinetic analysis of plasma and nasal ruprintrivir concentrations revealed intranasal drug residence with minimal systemic absorption. Results from these studies in experimental rhinoviral infection support continued investigation of intranasal ruprintrivir in the setting of natural HRV infection.

scholarly journals Pengaruh Kitosan Terhadap Jumlah Osteoklas dan Osteoblas Pada Tikus Galur Wistar Model Menopause

2017 ◽  
Vol 1 (2) ◽  
pp. 76-87
Author(s):  
Muhammad Nur
Keyword(s):  
Treatment Group ◽  
Total Sample ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference

Chitosan is one of the most abundant and dispersed polymers in nature. It has a rapid healing effect for tissues and has an effect on bone remodeling. The purpose of this study was to prove that giving chitosan influenced the decrease of osteoclast number and increasing number of osteoblasts in female mice of wistar strain of ovariectomy menopause model.This study used a posttest only control group design. The total sample of 30 rats divided into three groups was randomized, osteoporosis was made with the menopausal model which was performed ovariectomy in the positive control group and the treatment group then in the treatment group was given chitosan extract of single dose of 45 mg / day via Nasogastric Tube (NGT). K1 was a negative control group (without ovariectomy and without chitosan), K2 was a positive control group (with no chitosan ovariectomy), K3 was the treatment group (ovariectomy and chitosan 45 mg / day).The results showed significant differences in the number of osteoblasts between K1 and K3, K2 with K3, and no significant differences were found in the K1 group with K2. While the number of osteoclasts showed that there were significant differences in K1 group with K2 and no significant difference was found in K1 group with K3, K2 with K3.Conclusions in this study showed that 45 mg / day of chitosan doses could significantly increase osteoblast counts in menopause female white rats and decrease the number of osteoclasts performed by ovariectomy with chitosan administration.

scholarly journals Effectiveness of Boiled Figs (Ficus carica L), Rosy Periwinkle (Catharanthus roseus) and Cherry (Muntingia Calabura L.) on Decreasing SGOT and SGPT Serum in Male Wistar Strain Rats with Acute Hepatitis Model

2019 ◽  
Vol 7 (1) ◽  
pp. 744-755
Author(s):  
Grace Arni Yunita Hutajulu ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Catharanthus Roseus ◽  
Positive Control ◽  
Negative Control ◽  
Ficus Carica ◽  
Positive Control Group ◽  
Control Group ◽  
Wistar Strain ◽  
Muntingia Calabura

Acute hepatitis is inflammation of the liver. This disorder is usually caused by a virus or exposure to alcohol, drugs, toxic, or other pathogens. The purpose of this study was to determine the effectiveness of boiled figs leaves (Ficus carica L.), Rosy Periwinkle (Catharanthus roseus), and cherry (Muntingia Calabura L.) on decreasing serum levels of SGOT and SGPT. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. Rats were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 0.3 grams Figs (Ficus carica L.), 3.5 grams of rosy Periwinkle (Catharanthus roseus), and 3.5 grams of cherry (Muntingia Calabura L.) was given as much as 3.6 cc orally for 7 days to treatment group. Data were analyzed with SPSS version 24, One Way ANOVA test was performed to compare SGOT and SGPT levels. The results showed a significant difference in SGOT levels between the treatment group, positive control group, and negative control group (p <0.05) and there were significant differences in SGPT levels between the treatment group and positive control group (p <0.05). The conclusions of this study were mixed  boiled of Figs (Ficus carica L.), rosy Periwinkle (Catharanthus roseus), and and Cherry (Muntingia Calabura L.) had an effect in decreasing serum SGOT and SGPT in male rats with acute hepatitis model.

scholarly journals Effectiveness of Boiled Comfrey Leaves (Symphytum Officinale L) on SGOT SGPT Serum in Male Wistar Strain Rats With Acute Hepatitis Models

2019 ◽  
Vol 7 (1) ◽  
pp. 756-767
Author(s):  
Rois Sahidin ◽  
Untung Sudharmono
Keyword(s):  
Treatment Group ◽  
Acute Hepatitis ◽  
Serum Levels ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Positive Control Group ◽  
Control Group ◽  
Symphytum Officinale ◽  
Wistar Strain

Acute hepatitis is an acute disease caused by viruses, alcohol and drugs, which is characterized by an increase in SGOT SGPT serum. The purpose of this study was to determine the effectiveness of boiled comfrey leaves (Symphytum Officinale L) levels of SGOT and SGPT serum in male wistar strain rats with acute hepatitis models. The objects in this study were 30 male Wistar strain rats aged 2-3 months with a weight of 180-200 grams. The objects were randomly divided into 3 groups: the treatment group, positive control and negative control. The treatment group and positive control group were induced paracetamol 120 mg / day orally for 7 days. Boiled water of 2.8 grams of comfrey (symphytum officinale L) leaves was given as much as 2.7 cc orally for 7 days to the treatment group. Data were analyzed with SPSS version 24, one way ANOVA test was performed to compare serum levels of SGOT & SGPT. The results showed there were significant differences in serum levels of SGOT between the treatment group, positive control and negative control (p <0.05) and there were significant differences in SGPT results between the treatment group and positive control group (p <0.05) but there were no significant differences between the SGPT levels of the treatment group and the negative control group (p = 0.173). As Conclusion, boiled Comfrey leaf has an effect in decreasing serum SGOT & SGPT in male wistar strain rats with acute hepatitis model.

scholarly journals Liver Histopathological Change and Malondialdehyde Level of Rattus Norvegicus on Administration of Curcuma Zedoaria and Paracetamol Toxic Dose

2021 ◽  
Vol 3 (1) ◽  
pp. 38-46
Author(s):  
Putu Oky Ari Tania ◽  
Puja Ayu Misuari ◽  
Satya Yudhayana ◽  
Ketut Ayesha Edelwise Prayoga
Keyword(s):  
Body Weight ◽  
Free Radicals ◽  
Treatment Group ◽  
Rattus Norvegicus ◽  
Histopathological Change ◽  
Positive Control ◽  
Negative Control ◽  
Histological Structure ◽  
Curcuma Zedoaria ◽  
Toxic Dose

High doses of paracetamol create necrosis in the liver and produce free radicals. When liver function decreased in a long time, it will lead to severe liver damage and it will be irreversible. Rhizome of Curcuma zedoaria has the potential effect as an antioxidant, it is assumed that  its properties inhibit the formation of free radicals which formed from toxic doses of paracetamol. The aim of this study was to examine the histological structure of the liver and to determine MDA levels in the administration of C. zedoaria toxic dose and paracetamol on the Rattus norvegicus. The study was used twenty-four rats divided into four groups (positive control: CMC 0.5%; negative control: paracetamol 1.35g/kg body weight; treatment group 1 (T1): C. zedoaria 105 mg/200g and paracetamol 1.35g/kg Body weight 2 hours later, and treatment group 2 (T2): paracetamol 1.35g/ kg Body weight and C. zedoaria 105 mg/200g 2 hours later).  The Kruskall-Wallis test results showed MDA level did not significantly different between groups (p = 0.087). Hepatocellular changes were observed descriptively with Hematoxylin-eosin staining. Positive control showed greater hepatocellular changes rather than other groups, hepatocyte cells were enlarged with cytoplasm showing eosinophilic granules infiltrates, enlarged irregular nuclei, nucleolus prominent, there are many necrosis cells.

Sign in / Sign up

Export Citation Format

Share Document