scholarly journals Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications

2019 ◽  
Author(s):  
Arianna De Mori ◽  
Meena Hafidh ◽  
Natalia Mele ◽  
Rahmi Yusuf ◽  
Guido Cerri ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Composite Scaffold ◽  
Silver Nanowires ◽  
Gelation Time ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Bone Tissue Regeneration ◽  
Composite Gels ◽  
Prevention Of Infections

One-dimensional nanostructures such as silver nanowires (AgNWs) have attracted considerable attention owing to their outstanding electrical, thermal and antimicrobial properties; however, their application in the prevention of infections linked to bone tissue regeneration interventions has not yet been explored. Here we report on the development of an innovative scaffold prepared from chitosan, composite hydroxyapatite and AgNWs (CS-HACS-AgNWs) having both bioactive and antibacterial properties. In vitro results highlighted the antibacterial potential of AgNWs against both gram-positive and gram-negative bacteria. The CS-HACS-AgNWs composite scaffold demonstrated suitable Ca/P deposition, improved gel strength, reduced gelation time, and sustained Ag+ release within therapeutic concentrations. Antibacterial studies showed that the composite formulation was capable of inhibiting bacterial growth in suspension and of completely preventing biofilm formation on the scaffold in the presence of resistant strains. The hydrogels were also shown to be biocompatible, allowing cell proliferation. In summary, the developed CS-HACS-AgNWs composite hydrogels demonstrated significant potential as a scaffold material to be employed in bone regenerative medicine, as it presents enhanced mechanical strength combined with the ability to allow calcium salts deposition, while efficiently decreasing the risk of infections. The results presented justify further investigations into potential clinical applications of these materials.

scholarly journals Sustained Release from Injectable Composite Gels Loaded with Silver Nanowires Designed to Combat Bacterial Resistance in Bone Regeneration Applications

Pharmaceutics ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 116 ◽  
Author(s):  
Arianna De Mori ◽  
Meena Hafidh ◽  
Natalia Mele ◽  
Rahmi Yusuf ◽  
Guido Cerri ◽  
...  
Keyword(s):  
Bacterial Resistance ◽  
Composite Scaffold ◽  
Silver Nanowires ◽  
Gelation Time ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Bone Tissue Regeneration ◽  
Composite Gels ◽  
Prevention Of Infections

One-dimensional nanostructures, such as silver nanowires (AgNWs), have attracted considerable attention owing to their outstanding electrical, thermal and antimicrobial properties. However, their application in the prevention of infections linked to bone tissue regeneration intervention has not yet been explored. Here we report on the development of an innovative scaffold prepared from chitosan, composite hydroxyapatite and AgNWs (CS-HACS-AgNWs) having both bioactive and antibacterial properties. In vitro results highlighted the antibacterial potential of AgNWs against both gram-positive and gram-negative bacteria. The CS-HACS-AgNWs composite scaffold demonstrated suitable Ca/P deposition, improved gel strength, reduced gelation time, and sustained Ag+ release within therapeutic concentrations. Antibacterial studies showed that the composite formulation was capable of inhibiting bacterial growth in suspension, and able to completely prevent biofilm formation on the scaffold in the presence of resistant strains. The hydrogels were also shown to be biocompatible, allowing cell proliferation. In summary, the developed CS-HACS-AgNWs composite hydrogels demonstrated significant potential as a scaffold material to be employed in bone regenerative medicine, as they present enhanced mechanical strength combined with the ability to allow calcium salts deposition, while efficiently decreasing the risk of infections. The results presented justify further investigations into the potential clinical applications of these materials.

scholarly journals An In Vitro Coumarin-Antibiotic Combination Treatment of Pseudomonas aeruginosa Biofilms

2021 ◽  
Vol 16 (1) ◽  
pp. 1934578X2098774
Author(s):  
Jinpeng Zou ◽  
Yang Liu ◽  
Ruiwei Guo ◽  
Yu Tang ◽  
Zhengrong Shi ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drug Resistance ◽  
Combination Treatment ◽  
Crude Extract ◽  
Biofilm Formation ◽  
Bacterial Resistance ◽  
Antibacterial Properties ◽  
Biofilm Growth ◽  
Antibiotic Combination

The drug resistance of Pseudomonas aeruginosa is a worldwide problem due to its great threat to human health. A crude extract of Angelica dahurica has been proved to have antibacterial properties, which suggested that it may be able to inhibit the biofilm formation of P. aeruginosa; initial exploration had shown that the crude extract could inhibit the growth of P. aeruginosa effectively. After the adaptive dose of coumarin was confirmed to be a potential treatment for the bacteria’s drug resistance, “coumarin-antibiotic combination treatments” (3 coumarins—simple coumarin, imperatorin, and isoimperatorin—combined with 2 antibiotics—ampicillin and ceftazidime) were examined to determine their capability to inhibit P. aeruginosa. The final results showed that (1) coumarin with either ampicillin or ceftazidime significantly inhibited the biofilm formation of P. aeruginosa; (2) coumarin could directly destroy mature biofilms; and (3) the combination treatment can synergistically enhance the inhibition of biofilm formation, which could significantly reduce the usage of antibiotics and bacterial resistance. To sum up, a coumarin-antibiotic combination treatment may be a potential way to inhibit the biofilm growth of P. aeruginosa and provides a reference for antibiotic resistance treatment.

scholarly journals Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus

2013 ◽  
Vol 14 (5) ◽  
pp. 924-929 ◽  
Author(s):  
Reena Kulshrestha ◽  
J Kranthi ◽  
P Krishna Rao ◽  
Feroz Jenner ◽  
V Abdul Jaleel ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Antimicrobial Activity ◽  
Dental Health ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Test Organism ◽  
Diffusion Method ◽  
Zone Of Inhibition ◽  
Disk Diffusion Method

ABSTRACT Aim The present study was conducted to evaluate the efficacy of commercially available herbal toothpastes against the different periodontopathogens. Materials and methods Six herbal toothpastes that were commonly commercially available were included in the study. Colgate herbal, Babool, Meswak, Neem active, Dabur red toothpastes were tested for the study whereas sterile normal saline was used as control. Antimicrobial efficacies of dentifrices were evaluated against Streptococcus mutans and Actinobacillus actinomycetemcomitans. The antimicrobial properties of dentifrices were tested by measuring the maximum zone of inhibition at 24 hours on the Mueller Hinton Agar media inoculated with microbial strain using disk diffusion method. Each dentifrice was tested at 100% concentration (full strength). Results The study showed that all dentifrices selected for the study were effective against the entire test organism but to varying degree. Neem active tooth paste gave a reading of 25.4 mm as the zone of inhibition which was highest amongst all of the test dentifrices. Colgate Herbal and Meswak dentifrices recorded a larger maximum zone of inhibition, measuring 23 and 22.6 mm respectively, compared to other toothpastes. All other dentifrices showed the zone of inhibition to be between 17 and 19 mm respectively. Conclusion The antibacterial properties of six dentifrices were studied in vitro and concluded that almost all of the dentifrices available commercially had antibacterial properties to some extent to benefit dental health or antiplaque action. How to cite this article Jenner F, Jaleel VA, Kulshrestha R, Maheswar G, Rao PK, Kranthi J. Evaluating the Antimicrobial Activity of Commercially Available Herbal Toothpastes on Microorganisms Associated with Diabetes Mellitus. J Contemp Dent Pract 2013;14(5):924-929.

scholarly journals Injectable Alginate-Peptide Composite Hydrogel as a Scaffold for Bone Tissue Regeneration

Nanomaterials ◽  
2019 ◽  
Vol 9 (4) ◽  
pp. 497 ◽  
Author(s):  
Moumita Ghosh ◽  
Michal Halperin-Sternfeld ◽  
Itzhak Grinberg ◽  
Lihi Adler-Abramovich
Keyword(s):  
Extracellular Matrix ◽  
Bone Regeneration ◽  
Composite Scaffold ◽  
Three Dimensional ◽  
Composite Hydrogel ◽  
Bone Tissue Regeneration ◽  
Pcr Analysis ◽  
Alizarin Red ◽  
In Vitro Biocompatibility

The high demand for tissue engineering scaffolds capable of inducing bone regeneration using minimally invasive techniques prompts the need for the development of new biomaterials. Herein, we investigate the ability of Alginate incorporated with the fluorenylmethoxycarbonyl-diphenylalanine (FmocFF) peptide composite hydrogel to serve as a potential biomaterial for bone regeneration. We demonstrate that the incorporation of the self-assembling peptide, FmocFF, in sodium alginate leads to the production of a rigid, yet injectable, hydrogel without the addition of cross-linking agents. Scanning electron microscopy reveals a nanofibrous structure which mimics the natural bone extracellular matrix. The formed composite hydrogel exhibits thixotropic behavior and a high storage modulus of approximately 10 kPA, as observed in rheological measurements. The in vitro biocompatibility tests carried out with MC3T3-E1 preosteoblast cells demonstrate good cell viability and adhesion to the hydrogel fibers. This composite scaffold can induce osteogenic differentiation and facilitate calcium mineralization, as shown by Alizarin red staining, alkaline phosphatase activity and RT-PCR analysis. The high biocompatibility, excellent mechanical properties and similarity to the native extracellular matrix suggest the utilization of this hydrogel as a temporary three-dimensional cellular microenvironment promoting bone regeneration.

scholarly journals Sterilization Procedure for Temperature-Sensitive Hydrogels Loaded with Silver Nanoparticles for Clinical Applications

Nanomaterials ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 380 ◽  
Author(s):  
Diana Rafael ◽  
Fernanda Andrade ◽  
Francesc Martinez-Trucharte ◽  
Jana Basas ◽  
Joaquín Seras-Franzoso ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Biomedical Applications ◽  
Gelation Time ◽  
Antimicrobial Properties ◽  
High Sensitivity ◽  
Cost Effective ◽  
Raw Material ◽  
Temperature Sensitive ◽  
Dry Heat

Hydrogels (HG) have recognized benefits as drug delivery platforms for biomedical applications. Their high sensitivity to sterilization processes is however one of the greatest challenges regarding their clinical translation. Concerning infection diseases, prevention of post-operatory related infections is crucial to ensure appropriate patient recovery and good clinical outcomes. Silver nanoparticles (AgNPs) have shown good antimicrobial properties but sustained release at the right place is required. Thus, we produced and characterized thermo-sensitive HG based on Pluronic® F127 loaded with AgNPs (HG-AgNPs) and their integrity and functionality after sterilization by dry-heat and autoclave methods were carefully assessed. The quality attributes of HG-AgNPs were seriously affected by dry-heat methods but not by autoclaving methods, which allowed to ensure the required sterility. Also, direct sterilization of the final HG-AgNPs product proved more effective than of the raw material, allowing simpler production procedures in non-sterile conditions. The mechanical properties were assessed in post mortem rat models and the HG-AgNPs were tested for its antimicrobial properties in vitro using extremely drug-resistant (XDR) clinical strains. The produced HG-AgNPs prove to be versatile, easy produced and cost-effective products, with activity against XDR strains and an adequate gelation time and spreadability features and optimal for in situ biomedical applications.

Cu–Co Co-Doped Microporous Coating on Titanium with Osteogenic and Antibacterial Properties

2021 ◽  
Vol 17 (7) ◽  
pp. 1435-1447
Author(s):  
Quan-Ming Zhao ◽  
Bo Li ◽  
Fu-Xun Yu ◽  
Yan-Kun Li ◽  
Jie-Shi Wu ◽  
...  
Keyword(s):  
Clinical Performance ◽  
Antibacterial Properties ◽  
Microarc Oxidation ◽  
Antimicrobial Properties ◽  
Antibacterial Activities ◽  
Bone Surgery ◽  
Co Doping ◽  
Proliferation And Differentiation ◽  
Co Doped

Titanium (Ti) and its alloys are widely used in bone surgery by virtue of their excellent mechanical properties and good biocompatibility; however, complications such as loosening and sinking have been reported post-implantation. Herein we deposited a copper–cobalt (Cu–Co) co-doped titanium dioxide (TUO) coating on the surface of Ti implants by microarc oxidation. The osteogenic and antimicrobial properties of the coating were evaluated by in vitro experiments, and we also assessed β-catenin expression levels on different sample surfaces. Our results revealed that the coating promoted the adhesion, proliferation, and differentiation of MG63 osteoblasts, and TUO coating promoted β-catenin expression; moreover, the proliferation of Staphylococcus aureus was inhibited. To summarize, we report that Cu–Co co-doping can enhance the osteogenic and antibacterial activities of orthopedic Ti implants, leading to potentially improved clinical performance.

scholarly journals Osteoconductive 3D porous composite scaffold from regenerated cellulose and cuttlebone-derived hydroxyapatite

2018 ◽  
Vol 33 (6) ◽  
pp. 876-890 ◽  
Author(s):  
Alisa Palaveniene ◽  
Sedef Tamburaci ◽  
Ceren Kimna ◽  
Kristina Glambaite ◽  
Odeta Baniukaitiene ◽  
...  
Keyword(s):  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Regenerated Cellulose ◽  
Similar Amount ◽  
Coastal Zones ◽  
Micro Computed Tomography ◽  
Major Step ◽  
Culture Studies ◽  
Trace Elemental

Recently, usage of marine-derived materials in biomedical field has come into prominence due to their promising characteristics such as biocompatibility, low immunogenicity and wide accessibility. Among these marine sources, cuttlebone has been used as a valuable component with its trace elemental composition in traditional medicine. Recent studies have focused on the use of cuttlebone as a bioactive agent for tissue engineering applications. In this study, hydroxyapatite particles were obtained by hydrothermal synthesis of cuttlebone and incorporated to cellulose scaffolds to fabricate an osteoconductive composite scaffold for bone regeneration. Elemental analysis of raw cuttlebone material from different coastal zones and cuttlebone-derived HAp showed that various macro-, micro- and trace elements – Ca, P, Na, Mg, Cu, Sr, Cl, K, S, Br, Fe and Zn were found in a very similar amount. Moreover, biologically unfavorable heavy metals, such as Ag, Cd, Pb or V, were not detected in any cuttlebone specimen. Carbonated hydroxyapatite particle was further synthesized from cuttlebone microparticles via hydrothermal treatment and used as a mineral filler for the preparation of cellulose-based composite scaffolds. Interconnected highly porous structure of the scaffolds was confirmed by micro-computed tomography. The mean pore size of the scaffolds was 510 µm with a porosity of 85%. The scaffolds were mechanically characterized with a compression test and cuttlebone-derived HAp incorporation enhanced the mechanical properties of cellulose scaffolds. In vitro cell culture studies indicated that MG-63 cells proliferated well on scaffolds. In addition, cuttlebone-derived hydroxyapatite significantly induced the ALP activity and osteocalcin secretion. Besides, HAp incorporation increased the surface mineralization which is the major step for bone tissue regeneration.

scholarly journals SYNTHESIS, CHARACTERIZATION AND IN VITRO ANTIMICROBIAL EVALUATION OF SOME NOVEL BENZIMIDAZOLE DERIVATIVES BEARING HYDRAZONE MOIETY

2017 ◽  
Vol 10 (16) ◽  
pp. 1
Author(s):  
Jayanta Sarma ◽  
Gurvinder Singh ◽  
Mukta Gupta ◽  
Reena Gupta ◽  
Bhupinder Kapoor
Keyword(s):  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Aromatic Aldehydes ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Benzimidazole Derivatives ◽  
E Coli ◽  
Antimicrobial Evaluation ◽  
Antibacterial Potential

Objective: The synthesis of novel benzimidazole-hydrazone derivatives has been carried out based on the previous findings that both these pharmacophores possess potent antimicrobial activities. The antibacterial properties of synthesized derivatives were screened against both Gram-positive and Gram-negative bacteria.Methods: O-phenylenediamine on condensation with substituted aromatic acids in polyphosphoric acid gave benzimidazole nucleus which on reaction with ethyl chloroacetate and hydrazine hydrate in two different steps resulted in the formation of substituted acetohydrazides. The targeted compounds 6a-l were synthesized by reaction of substituted acetohydrazides with aromatic aldehydes and screened for their antibacterial potential by cup-plate method.Results: The synthesized benzimidazole-hydrazones exhibited moderate to strong antibacterial activities against Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeruginosa. The compounds 6a-6f were found to be most effective against S. aureus, E. coli, and P. aeruginosa. Among all the synthesized compounds, the zone of inhibition of 6f in highest concentration, i.e., 100 μg/ml were found to be >31 mm against all the stains of bacteria.Conclusion: The antibacterial results revealed that the synthetized derivatives have significant antimicrobial properties and further structure activity relationship studies may develop more potent and less toxic molecules.

scholarly journals Synthesis of Copper Nanoparticles by Thermal Decomposition and Their Antimicrobial Properties

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
R. Betancourt-Galindo ◽  
P. Y. Reyes-Rodriguez ◽  
B. A. Puente-Urbina ◽  
C. A. Avila-Orta ◽  
O. S. Rodríguez-Fernández ◽  
...  
Keyword(s):  
Thermal Decomposition ◽  
Copper Nanoparticles ◽  
Copper Chloride ◽  
Metallic Copper ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Face Centered Cubic ◽  
Bacterial Strains ◽  
Tem Analysis

Copper nanoparticles were synthesized by thermal decomposition using copper chloride, sodium oleate, and phenyl ether as solvent agents. The formation of nanoparticles was evidenced by the X-ray diffraction and transmission electron microscopy. The peaks in the XRD pattern correspond to the standard values of the face centered cubic (fcc) structure of metallic copper and no peaks of other impurity crystalline phases were detected. TEM analysis showed spherical nanoparticles with sizes in the range of 4 to 18 nm. The antibacterial properties of copper nanoparticles were evaluatedin vitroagainst strains ofStaphylococcus aureusandPseudomonas aeruginosa. The antibacterial activity of copper nanoparticles synthesized by thermal decomposition showed significant inhibitory effect against these highly multidrug-resistant bacterial strains.

scholarly journals Antibacterial Properties of Triethoxysilylpropyl Succinic Anhydride Silane (TESPSA) on Titanium Dental Implants

Polymers ◽  
2020 ◽  
Vol 12 (4) ◽  
pp. 773
Author(s):  
Judit Buxadera-Palomero ◽  
Maria Godoy-Gallardo ◽  
Meritxell Molmeneu ◽  
Miquel Punset ◽  
Francisco Javier Gil
Keyword(s):  
Dental Implants ◽  
Photoelectron Spectroscopy ◽  
Succinic Anhydride ◽  
Human Fibroblasts ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Sodium Titanate ◽  
Adhesion Assay ◽  
Vitro Effect

Infections related to dental implants are a common complication that can ultimately lead to implant failure, and thereby carries significant health and economic costs. In order to ward off these infections, this paper explores the immobilization of triethoxysilylpropyl succinic anhydride (TESPSA, TSP) silane onto dental implants, and the interaction of two distinct monospecies biofilms and an oral plaque with the coated titanium samples. To this end, titanium disks from prior machining were first activated by a NaOH treatment and further functionalized with TESPSA silane. A porous sodium titanate surface was observed by scanning electron microscopy and X-ray photoelectron spectroscopy analyses confirmed the presence of TESPSA on the titanium samples (8.4% for Ti–N-TSP). Furthermore, a lactate dehydrogenase assay concluded that TESPSA did not have a negative effect on the viability of human fibroblasts. Importantly, the in vitro effect of modified surfaces against Streptococcus sanguinis, Lactobacillus salivarius and oral plaque were studied using a viable bacterial adhesion assay. A significant reduction was achieved in all cases but, as expected, with different effectiveness against simple mono-species biofilm (ratio dead/live of 0.4) and complete oral biofilm (ratio dead/live of 0.6). Nevertheless, this approach holds a great potential to provide dental implants with antimicrobial properties.

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